A traditional Chinese medicine composition for improving heart failure and a preparation method thereof

Abstract

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for improving heart failure and a preparation method thereof. The traditional Chinese medicine composition for improving heart failure comprises the following raw materials in parts by weight: astragaloside A 4-7 parts, arctiin 8-12 parts, rosmarinecin 3-6 parts, and sclareolide 2-4 parts. Test results show that by taking the traditional Chinese medicine composition for improving heart failure prepared by the application, the efficacy of tonifying heart qi, warming yang and kidney, promoting blood circulation and resolving toxin, and eliminating swelling and promoting diuresis can be achieved, so that the heart function can be effectively improved, the over-activation of the RAAS system can be inhibited, the myocardial injury and myocardial fibrosis can be reduced, and the symptoms of heart failure can be significantly relieved. The traditional Chinese medicine composition provided by the application is compatible and precise, has small toxic and side effects, is safe, and has a wide application prospect.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a traditional Chinese medicine composition for improving heart failure and its preparation method. Background Technology

[0002] Heart failure is a complex clinical syndrome characterized by a range of symptoms and signs resulting from structural or functional abnormalities of the heart that lead to impaired ventricular contraction or filling. As a severe stage and end-stage of various heart diseases, heart failure is characterized by high morbidity, high disability rates, and high mortality rates. It not only seriously damages patients' physical health and quality of life but also places a heavy burden on patients' families and the social healthcare system. Therefore, heart failure has become a pressing clinical challenge that needs to be addressed.

[0003] Currently, Western medicine's treatment philosophy for heart failure has undergone a significant shift. Traditionally, Western medicine employed short-term symptomatic treatment, rapidly improving myocardial contractility through methods such as diuretics, cardiotonics, and vasodilators. However, at present, the treatment strategy is gradually transitioning to long-term restorative therapy, primarily using neuroendocrine inhibitors. The core objective is to regulate neuroendocrine disorders, delay cardiac remodeling, and thereby improve the patient's long-term prognosis.

[0004] Traditional Chinese medicine (TCM) has unique advantages in treating heart failure. Its core treatment philosophy lies in syndrome differentiation and treatment based on a holistic approach, emphasizing a holistic approach that addresses the patient's overall health. By regulating the flow of Qi, blood, and body fluids, it can improve clinical symptoms such as chest tightness, shortness of breath, and edema in heart failure patients, while also enhancing the body's resistance and reducing the risk of relapse caused by factors such as infection and fatigue.

[0005] There is an urgent need for a traditional Chinese medicine composition that is effective, safe, and easy to prepare to improve heart failure, providing better treatment options for heart failure patients. Based on this, the present invention provides a traditional Chinese medicine composition for improving heart failure and its preparation method. Summary of the Invention

[0006] The primary objective of this invention is to provide a traditional Chinese medicine composition for improving heart failure.

[0007] The second objective of this invention is to provide a method for preparing a traditional Chinese medicine composition for improving heart failure.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows: A traditional Chinese medicine composition for improving heart failure, the composition comprising the following raw materials in parts by weight: 4-7 parts astragaloside A, 8-12 parts arctiin, 3-6 parts citrinin, and 2-4 parts perillaldehyde.

[0009] Furthermore, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5 parts astragaloside A, 10 parts arctiin, 4 parts citrinin, and 3 parts perilla lactone.

[0010] The preparation method of the traditional Chinese medicine composition for improving heart failure as described above specifically includes the following steps: (1) Weigh each raw material according to the weight proportions: astragaloside A, arctiin, citric acid, and perilla lactone, sieve, and prepare Chinese medicine powder for later use; (2) Mix the Chinese herbal powder obtained in step (1) evenly to obtain mixed herbal powder; (3) Add filler, disintegrant and lubricant to the mixed powder prepared in step (2), dry granulate and compress into tablets to obtain the traditional Chinese medicine composition.

[0011] Furthermore, the mesh size of the sieve in step (1) is 80-100.

[0012] Furthermore, in step (2), the mixing speed is 20-40 r / min; the mixing time is 10-20 min.

[0013] Furthermore, in step (3), the filler is microcrystalline cellulose and lactose; the disintegrant is sodium carboxymethyl starch; and the lubricant is magnesium stearate.

[0014] Further, the weight ratio of the mixed powder: filler: disintegrant: lubricant is 1:(2-4):(0.05-0.1):(0.01-0.02).

[0015] Furthermore, the weight ratio of microcrystalline cellulose to lactose in the filler is (1-3):1.

[0016] Compared with the prior art, the main advantages of the present invention are as follows: This invention provides a traditional Chinese medicine composition for improving heart failure and its preparation method. It scientifically combines four active ingredients—astragaloside A, arctiin, lanolin, and perilla lactone—to achieve the effects of tonifying heart qi, warming and tonifying the kidneys, promoting blood circulation and detoxification, and reducing swelling and promoting diuresis, producing a significant synergistic effect. Experimental results show that taking the traditional Chinese medicine composition prepared according to this invention effectively improves cardiac function, inhibits excessive activation of the RAAS system, reduces myocardial damage and myocardial fibrosis, and significantly alleviates heart failure symptoms. Furthermore, the traditional Chinese medicine composition provided by this invention has precise formulation, few toxic side effects, and high safety, showing broad application prospects. Attached Figure Description

[0017] Figure 1 The effect of different groups of traditional Chinese medicine compositions on the NT-proBNP content in rat serum; Figure 2 The effect of different groups of traditional Chinese medicine compositions on the AngII content in rat serum; Figure 3 The expression level of TGF-β1 protein in rat myocardial tissue was detected by Western blotting. Detailed Implementation

[0018] The technical solution of the present invention will be further described below with reference to specific embodiments. However, those skilled in the art should understand that the following embodiments are only for illustrating the present invention and should not be regarded as limiting the present invention. Specific conditions not specified in the embodiments are performed according to conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the reagents or instruments used are all conventional products obtained through commercial channels.

[0019] The astragaloside A, arctiin, citric acid, and perilla lactone used in the embodiments of this invention were all purchased from Shanghai Maclean Biochemical Technology Co., Ltd.

[0020] Example 1 A traditional Chinese medicine composition for improving heart failure, the composition comprising the following raw materials in parts by weight: 5 parts astragaloside A, 10 parts arctiin, 4 parts citrinin, and 3 parts perilla lactone.

[0021] A method for preparing a traditional Chinese medicine composition for improving heart failure specifically includes the following steps: (1) Weigh each raw material according to the following weight proportions: 5 parts of astragaloside A, 10 parts of arctiin, 4 parts of citric acid, and 3 parts of styracil lactone. Pass them through a 90-mesh sieve to obtain Chinese medicine powder for later use. (2) Place the Chinese medicine powder obtained in step (1) in a stirrer and mix it evenly for 15 minutes at a speed of 30 r / min to obtain mixed medicine powder; (3) Add filler, disintegrant and lubricant to the mixed powder prepared in step (2), granulate by dry method and compress into tablets to obtain the traditional Chinese medicine composition; wherein the filler is microcrystalline cellulose and lactose, and the weight ratio of microcrystalline cellulose to lactose is 2:1; the disintegrant is sodium carboxymethyl starch; the lubricant is magnesium stearate; the weight ratio of the mixed powder: filler: disintegrant: lubricant is 1:3:0.07:0.01.

[0022] Example 2 A traditional Chinese medicine composition for improving heart failure, the composition comprising the following raw materials in parts by weight: 4 parts astragaloside A, 8 parts arctiin, 3 parts citrinin, and 2 parts perilla lactone.

[0023] A method for preparing a traditional Chinese medicine composition for improving heart failure specifically includes the following steps: (1) Weigh each raw material according to the following weight proportions: 4 parts of astragaloside A, 8 parts of arctiin, 3 parts of citric acid, and 2 parts of styracil lactone. Pass them through an 80-mesh sieve to obtain Chinese medicine powder for later use. (2) Place the Chinese medicine powder obtained in step (1) in a stirrer and mix it evenly for 10 minutes at a speed of 20 r / min to obtain mixed medicine powder; (3) Add filler, disintegrant and lubricant to the mixed powder prepared in step (2), dry granulate and compress into tablets to obtain the traditional Chinese medicine composition; wherein the filler is microcrystalline cellulose and lactose, and the weight ratio of microcrystalline cellulose to lactose is 1:1; the disintegrant is sodium carboxymethyl starch; the lubricant is magnesium stearate; the weight ratio of the mixed powder: filler: disintegrant: lubricant is 1:2:0.05:0.01.

[0024] Example 3 A traditional Chinese medicine composition for improving heart failure, the composition comprising the following raw materials in parts by weight: 7 parts astragaloside A, 12 parts arctiin, 6 parts citrinin, and 4 parts perilla lactone.

[0025] A method for preparing a traditional Chinese medicine composition for improving heart failure specifically includes the following steps: (1) Weigh each raw material according to the following weight proportions: 7 parts of astragaloside A, 12 parts of arctiin, 6 parts of citric acid, and 4 parts of styrax lactone. Pass them through a 100-mesh sieve to obtain Chinese medicine powder for later use. (2) Place the Chinese medicine powder obtained in step (1) in a stirrer and mix it evenly for 20 minutes at a speed of 40 r / min to obtain mixed medicine powder; (3) Add filler, disintegrant and lubricant to the mixed powder prepared in step (2), dry granulate and compress into tablets to obtain the traditional Chinese medicine composition; wherein the filler is microcrystalline cellulose and lactose, and the weight ratio of microcrystalline cellulose to lactose is 3:1; the disintegrant is sodium carboxymethyl starch; the lubricant is magnesium stearate; the weight ratio of the mixed powder: filler: disintegrant: lubricant is 1:4:0.1:0.02.

[0026] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that the calyx ethylsin in a traditional Chinese medicine composition for improving heart failure is omitted, while all other components are the same as in Example 1.

[0027] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that the styracil lactone in a traditional Chinese medicine composition for improving heart failure is omitted, while all other aspects are the same as in Example 1.

[0028] Experimental Example 1 (1) By establishing a heart failure model in SD rats, the effects of different groups of traditional Chinese medicine compositions for improving heart failure prepared in this invention on cardiac function and related indicators in SD rats were investigated. The specific experimental steps are as follows: 120 SPF-grade SD rats aged 1.5 months and weighing 150±20g were selected for the experiment. Ten rats were used as blank control group, and the remaining 110 rats were used to construct a heart failure model by coronary artery ligation. After surgery, the rats were fed for 8 weeks and fasted for 12 hours. The skin of the rat chest was shaved and the left ventricular ejection fraction (LVEF) was recorded. Rats with LVEF<55% were established as the heart failure model group. The heart failure model rats that met the standard were randomly divided into positive control group, model group, low-dose group of Example 1, medium-dose group of Example 1, high-dose group of Example 1, low-dose group of Example 2, low-dose group of Example 3, low-dose group of Comparative Example 1, and low-dose group of Comparative Example 2, with 10 rats in each group.

[0029] (2) After the groups were formed, drug intervention was performed at 10:00 a.m. every day: Example 1 High-dose group: 8g / kg of the traditional Chinese medicine composition for improving heart failure in Example 1 was administered by gavage once a day for four consecutive weeks.

[0030] In Example 1, the dosage group was administered 4 g / kg of the traditional Chinese medicine composition for improving heart failure in Example 1 via gavage, once a day, for four consecutive weeks.

[0031] Example 1 Low-dose group: 2g / kg of the traditional Chinese medicine composition for improving heart failure in Example 1 was administered by gavage once a day for four consecutive weeks.

[0032] Example 2 Low-dose group: 2g / kg of the traditional Chinese medicine composition for improving heart failure in Example 2 was administered by gavage once a day for four consecutive weeks.

[0033] Example 3 Low-dose group: 2g / kg of the traditional Chinese medicine composition for improving heart failure in Example 3 was administered by gavage once a day for four consecutive weeks.

[0034] Comparative Example 1 Low-dose group: 2 g / kg of the traditional Chinese medicine composition for improving heart failure in Comparative Example 1 was administered by gavage once a day for four consecutive weeks.

[0035] Comparative Example 2 Low-dose group: 2 g / kg of the traditional Chinese medicine composition for improving heart failure in Comparative Example 2 was administered by gavage once a day for four consecutive weeks.

[0036] Blank control group: The same volume of normal saline was administered by gavage once a day for four consecutive weeks.

[0037] Positive control group: Captopril (12.5 mg / kg body weight) was administered by gavage once a day for four weeks.

[0038] Model group: The same volume of physiological saline was administered by gavage once a day for four consecutive weeks.

[0039] (3) After the last gavage, the left ventricular ejection fraction (LVEF) of the rats was measured using a high-resolution small animal ultrasound detection system. The results are shown in Table 1. The levels of NT-proBNP and AngII in the rat serum were detected by ELISA to assess the degree of RAAS system activation. The results are shown in Table 1. Figure 1 , Figure 2 As shown in the figure. Left ventricular myocardial tissue was collected, and proteins were extracted. Using GAPDH as an internal control, the expression level of TGF-β1 protein in rat myocardial tissue was detected by Western blotting to further explore its anti-myocardial fibrosis mechanism. The results are as follows. Figure 3 As shown.

[0040] Table 1. Effects of different groups of traditional Chinese medicine compositions on left ventricular ejection fraction (LVEF) in rats The results are shown in Table 1, which illustrates the effects of different groups of traditional Chinese medicine (TCM) compositions on left ventricular ejection fraction (LVEF) in rats. Table 1 shows that compared to the blank control group, the LVEF of the model group rats was significantly reduced, indicating that cardiac function was significantly impaired after heart failure. After 4 weeks of administration, compared to the model group, Comparative Example 1, and Comparative Example 2, the TCM compositions prepared in Examples 1-3 of this invention significantly increased the LVEF in rats, demonstrating that the TCM compositions can effectively improve cardiac function in rats with heart failure.

[0041] The results are as follows Figure 1 The figure shows the effect of different groups of traditional Chinese medicine compositions on the NT-proBNP content in rat serum. Figure 1 It was found that, compared with the blank control group, the serum NT-proBNP level in the model group rats was significantly increased. NT-proBNP is a core biomarker for the diagnosis and efficacy evaluation of heart failure, indicating impaired cardiac function and heart failure in rats. Compared with the model group, comparative example 1, and comparative example 2, the traditional Chinese medicine compositions prepared in Examples 1-3 of this invention can significantly reduce the serum NT-proBNP level in rats, indicating that the traditional Chinese medicine compositions can significantly improve cardiac function in rats with heart failure.

[0042] The results are as follows Figure 2 The figure shows the effect of different groups of traditional Chinese medicine compositions on the AngII content in rat serum. Figure 2It was found that, compared with the blank control group, the serum AngII levels of rats in all other groups were significantly increased. AngII is a core key factor in the activation of the RAAS system, and its increased level directly indicates that the RAAS system is in an overactivated state. Compared with the model group, comparative example 1 group, and comparative example 2 group, the traditional Chinese medicine compositions prepared in Examples 1-3 of this invention can significantly reduce the serum AngII levels of rats, indicating that the traditional Chinese medicine compositions can inhibit the overactivation of the RAAS system, reduce AngII-mediated ventricular remodeling, alleviate myocardial damage, and protect cardiac function.

[0043] The results are as follows Figure 3 The image shows the expression level of TGF-β1 protein in myocardial tissue as detected by Western blotting. Figure 3 It can be seen that, compared with the model group, comparative example 1, and comparative example 2, the traditional Chinese medicine compositions prepared in Examples 1-3 of this invention can significantly reduce the expression level of TGF-β1 protein in rat myocardial tissue. This indicates that the traditional Chinese medicine compositions of this invention can effectively reduce the degree of myocardial fibrosis, improve ventricular remodeling, and further protect the cardiac function of rats with heart failure by significantly downregulating the expression of TGF-β1 protein and inhibiting the proliferation of myocardial fibroblasts and collagen deposition.

[0044] In summary, the traditional Chinese medicine compositions for improving heart failure prepared in Examples 1-3 of this invention have the effects of tonifying heart qi, warming yang and tonifying kidney, promoting blood circulation and detoxifying, and reducing swelling and promoting diuresis. These compositions effectively improve cardiac function, inhibit excessive activation of the RAAS system, reduce myocardial damage and myocardial fibrosis, and significantly alleviate symptoms of heart failure. Furthermore, the traditional Chinese medicine compositions provided by this invention have precise formulation, few toxic side effects, and high safety, and have broad application prospects.

[0045] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. Based on the present invention, some modifications or substitutions can be made, but these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of protection claimed by the present invention.

Claims

1. A traditional Chinese medicine composition for improving heart failure, characterized in that, The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 4-7 parts astragaloside A, 8-12 parts arctiin, 3-6 parts citrinin, and 2-4 parts perilla lactone.

2. The traditional Chinese medicine composition for improving heart failure according to claim 1, characterized in that, The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5 parts astragaloside A, 10 parts arctiin, 4 parts citrinin, and 3 parts perilla lactone.

3. A method for preparing a traditional Chinese medicine composition for improving heart failure according to claim 1 or 2, characterized in that, Specifically, the following steps are included: (1) Weigh each raw material according to the weight proportions: astragaloside A, arctiin, citric acid, and perilla lactone, sieve, and prepare Chinese medicine powder for later use; (2) Mix the Chinese herbal powder obtained in step (1) evenly to obtain mixed herbal powder; (3) Add filler, disintegrant and lubricant to the mixed powder prepared in step (2), dry granulate and compress into tablets to obtain the traditional Chinese medicine composition.

4. The method for preparing a traditional Chinese medicine composition for improving heart failure according to claim 3, characterized in that, The mesh size of the sieve in step (1) is 80-100.

5. The method for preparing a traditional Chinese medicine composition for improving heart failure according to claim 3, characterized in that, The mixing speed in step (2) is 20-40 r / min; the mixing time is 10-20 min.

6. The method for preparing a traditional Chinese medicine composition for improving heart failure according to claim 3, characterized in that, In step (3), the filler is microcrystalline cellulose and lactose; the disintegrant is sodium carboxymethyl starch; and the lubricant is magnesium stearate.

7. The method for preparing a traditional Chinese medicine composition for improving heart failure according to claim 3, characterized in that, The weight ratio of the mixed powder, filler, disintegrant, and lubricant is 1:(2-4):(0.05-0.1):(0.01-0.02).

8. A method for preparing a traditional Chinese medicine composition for improving heart failure according to claim 6, characterized in that, The weight ratio of microcrystalline cellulose to lactose in the filler is (1-3):1.

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