Use of cytochrome p450 enzymes in the preparation of organogermanium compounds and methods
CN122357646BActive Publication Date: 2026-10-27SHANDONG UNIV
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Patent Information
- Application Number
- CN202610829407.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2026-06-10
- Publication Date
- 2026-10-27
- Estimated Expiration
- 2046-06-10
AI Technical Summary
Benefits of technology
[0013]本发明提供了一种利用 P450 BM3-F87A突变体蛋白制备有机锗化合物（外消旋体）的方法，具有显著的技术优势。该方法利用细胞色素 P450 BM3-F87A突变体蛋白宽大的活性口袋催化生成有机锗化合物外消旋体，使其在催化卡宾插入 Ge-H 键构建手性中心的过程中不具备显著的立体选择性，从而引导产物以对映异构体 1:1 的比例生成外消旋体。本发明利用生物工程技术构建的重组微生物实现有机锗化合物外消旋体的大规模合成，降低了成本；此外，该方法全过程在常温水相中进行，反应条件温和、安全环保，完全符合绿色化学的发展要求。
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Abstract
The application belongs to the technical field of enzyme catalysis, and relates to application and a method of cytochrome P450 in preparation of an organic germanium compound. The method utilizes a wide active pocket of a cytochrome P450 BM3-F87A mutant protein to catalyze generation of a racemic body of the organic germanium compound, so that the racemic body is not provided with significant stereoselectivity in a process of catalyzing a carbene to insert a Ge-H bond to construct a chiral center, so as to guide the product to generate the racemic body in a 1:1 ratio of enantiomers. The application realizes large-scale synthesis of the racemic body of the organic germanium compound by using a recombinant microorganism constructed by a biological engineering technology, and cost is reduced. In addition, the whole process is carried out in an aqueous solution at room temperature, and the reaction condition is mild, safe and environment-friendly, and completely meets the development requirement of green chemistry.
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