A miticidal composition containing a substituted phenyl sulfide compound and use thereof

Abstract

The application belongs to the technical field of pesticide mite-killing, and discloses a mite-killing composition containing a substituted phenyl sulfide compound and purposes thereof. The mite-killing composition contains active ingredient A and active ingredient B. The active ingredient A is a compound shown in formula I: (I). The active ingredient B is a mite-killing agent. The mite-killing composition has a remarkable mite-killing effect, can effectively control the harm of mites to crops, and slow down the generation and development of mite resistance.

Description

[0001] This invention application is a divisional application of application number 2024110903253, filed on August 9, 2024, entitled "An acaricide composition containing substituted phenyl sulfide compounds and its use therein". Technical Field

[0002] This invention belongs to the field of pesticide acaricide technology, and discloses an acaricide composition containing substituted phenyl sulfide compounds and its uses. Background Technology

[0003] Two-spotted spider mite, belonging to the genus *Tetranychus* of the family Tetranychidae, is an important global pest mite with a wide distribution and numerous host species. It has a stronger reproductive capacity and greater resistance to pesticides than pests such as the apple spider mite and the hawthorn spider mite. Adults and nymphs congregate on the underside of leaves to suck sap, while spinning webs on the leaf surface. Initially, small grayish-white spots appear on the leaves, later developing into red spots. In severe cases, the leaves turn rusty and dry, resembling burnt areas, and plant growth is inhibited. Due to its rapid reproduction, strong pesticide resistance, and excellent concealment, the two-spotted spider mite has developed varying degrees of resistance and cross-resistance to multiple acaricides.

[0004] Compound I is a substituted phenyl sulfide compound that exhibits excellent acaricidal effects against various plant mites. Its structural formula is as follows: (I).

[0005] The applicant conducted research on the compound of formula I and surprisingly discovered that when it was combined with certain acaricides in appropriate proportions, it had a good effect on delaying the development of resistance in two-spotted spider mites and also had excellent acaricidal effect on two-spotted spider mites. Summary of the Invention

[0006] Based on the above, the purpose of this invention is to provide an acaricide composition containing a substituted phenyl sulfide compound and its use. The acaricide composition contains a compound of formula I and another acaricide, which can be applied to control mites on crops, fruit trees, vegetables, flowers, forest trees and other plants. The acaricide has a significant synergistic effect on mites, especially effective against two-spotted spider mites. It is safe for crops, environmentally friendly, reduces the dosage of the agent, and lowers production costs.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: an acaricide composition containing a substituted phenyl sulfide compound, wherein the acaricide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound represented by formula I: (I) The active ingredient B is selected from any of the following acaricides: mitochondrial respiration inhibitors, growth inhibitors, neurotoxins, and other acaricides; Furthermore, the active ingredient A is a compound represented by Formula I: (I) The active ingredient B is selected from mitochondrial respiration inhibitors: acetaminophen, vaniliprole, fipronil, quinacrine, chlorfenapyr, bromfenac, difenoconazole, pyridaben, pyrimethanil, pyridaben, pyrimethanil, pyribumide, nicofluprole, fometoquin, quinacrine, pyrimethanil, flufenoxuron, pyrimethanil, chlorfenapyr, bromfenac, pyrimethanil, diflubenzuron, fluazinam, pyridaben; Growth inhibitor acaricides: Tetramethrin, Flufenoxam, Spirotetramethrin, Spirodiclofen, Spirodiclofen, Spirodiclofen diester, Tifenoxam, Tifenoxam, Etoxazole, Fluxametamide, Isocycloseram, Cyclopyralid, Lufenuron, Flufenoxuron, Flufenoxuron, Thiamethoxam; Neurotoxic acaricides: abamectin, methyl abamectin benzoate, liuyangmycin, huaguangmycin, mibamectin, bifenazate, high-efficiency cypermethrin, deltamethrin, cypermethrin, cypermethrin, bifenthrin, flufenoxuron, bromofenoxuron, triazole tin, fenbutatin, acynonapyr; Other acaricides: any one of the following: propargite, flupentiofenox, amidoflumet, matrine, veratrine, azadirachtin, pyrethroids, rotenone, pine miticide, olive miticide, mivorilaner, modoflaner, tigolaner, umifoxolaner, and trifluralin; Furthermore, the active ingredient B is selected from any one of the following: abamectin, spirodiclofen, bifenazate, etoxazole, cyprodinil, abamectin, emamectin benzoate, difenoconazole, pyridaben, fluazinam, spirodiclofen, etoxazole, spirotetramat, propargite, tetradifon, diflubenzuron, chlorfenapyr, lufenuron, bifenthrin, flufenoxuron, thiamethoxam, and spirodiclofen diester. Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:45 to 40:1; Furthermore, the active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:40~20:1, preferably 1:30~20:1. Alternatively, the active ingredient B is bifenthrin, and the mass ratio of active ingredient A to active ingredient B is 1:40~35:1, preferably 1:25~17:1; Alternatively, the active ingredient B is fluopyram, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 28:1, preferably 1:20 to 20:1. Alternatively, the active ingredient B is cypermethrin, and the mass ratio of active ingredient A to active ingredient B is 1:35~25:1, preferably 1:20~25:1; Alternatively, the active ingredient B is avermectin, and the mass ratio of active ingredient A to active ingredient B is 1:32~45:1, preferably 1:20~45:1. Alternatively, the active ingredient B is butyl ether urea, and the mass ratio of the active ingredient A to the active ingredient B is 1:30~42:1, preferably 1:15~42:1; Alternatively, the active ingredient B is cypermethrin, and the mass ratio of active ingredient A to active ingredient B is 1:35~45:1, preferably 1:15~45:1. Alternatively, the active ingredient B is biphenylhydrazine ester, and the mass ratio of active ingredient A to active ingredient B is 1:30~40:1, preferably 1:15~40:1; Alternatively, the active ingredient B is pyridaben, and the mass ratio of active ingredient A to active ingredient B is 1:40~35:1, preferably 1:40~22:1; Alternatively, the active ingredient B is etoxazole, and the mass ratio of active ingredient A to active ingredient B is 1:32~48:1, preferably 1:16~24:1; Alternatively, the active ingredient B is azoxystrobin, and the mass ratio of active ingredient A to active ingredient B is 1:45~50:1, preferably 1:45~30:1. Alternatively, the active ingredient B is triazole tin, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 33:1, preferably 1:20 to 10:1. Alternatively, the active ingredient B is propargite, and the mass ratio of active ingredient A to active ingredient B is 1:50~50:1, preferably 1:50~30:1; Alternatively, the active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:30~45:1, preferably 1:15~45:1; Alternatively, the active ingredient B is lufenuron, and the mass ratio of the active ingredient A to the active ingredient B is 1:40~45:1, preferably 1:20~30:1; Alternatively, the active ingredient B is fluazinam, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 30:1, preferably 1:24 to 20:1. Alternatively, the active ingredient B is spirotetramat, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 30:1, preferably 1:20 to 20:1. Alternatively, the active ingredient B is dicofol, and the mass ratio of active ingredient A to active ingredient B is 1:32~44:1, preferably 1:16~34:1. Alternatively, the active ingredient B is high-efficiency cyhalothrin, and the mass ratio of active ingredient A to active ingredient B is 1:42~32:1, preferably 1:36~24:1; Alternatively, the active ingredient B is emamectin benzoate, and the mass ratio of active ingredient A to active ingredient B is 1:40~42:1, preferably 1:20~32:1. Alternatively, the active ingredient B is thiamethoxam, and the mass ratio of the active ingredient A to the active ingredient B is 1:30~35:1, preferably 1:20~25:1; Alternatively, the active ingredient B is etoxazole, and the mass ratio of active ingredient A to active ingredient B is 1:30~32:1, preferably 1:20~24:1; Alternatively, the active ingredient B is tetradifon, and the mass ratio of active ingredient A to active ingredient B is 1:36~35:1, preferably 1:36~25:1; Alternatively, the active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:33~30:1, preferably 1:20~20:1; Furthermore, based on a total weight of 100 wt%, the total weight of active ingredient A and active ingredient B accounts for 2% to 90% of the total weight of the acaricidal composition, preferably 2% to 60%. Furthermore, in addition to the active ingredient, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers. The wetting agent is selected from one or more of the following: alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or The dispersant is selected from one or more of the following: lignin sulfonates, alkyl naphthalene sulfonates formaldehyde condensates, naphthalene sulfonates, tristyrylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensates sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or The emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or Antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or Defoamer selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or The solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil, vegetable oil derivatives, and water; and / or The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or The stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or Synergists are selected from synergistic phosphorus, synergistic ether; and / or The carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives. The carrier can be a solid carrier or a liquid carrier; The solid carrier includes: minerals, plant materials, synthetic fillers, and inorganic salts; The minerals mentioned include silicates, carbonates, sulfates, and oxides.

[0008] The silicates are selected from at least one of kaolin, sepiolite, pearl clay, montmorillonite, mica, vermiculite, pyrophyllite, and talc; the carbonates are selected from calcium carbonate or dolomite; the sulfates are selected from at least one of ammonium sulfate, sodium sulfate, and calcium sulfate; the oxides are selected from at least one of quicklime, magnesium lime, and diatomaceous earth; the plant materials are selected from at least one of citrus pomace, corn cob, rice husk powder, rice husk, soybean straw powder, tobacco powder, walnut shell, and sawdust; the synthetic fillers are selected from at least one of precipitated calcium carbonate hydrate, precipitated calcium carbonate, and silica; and the inorganic salts are selected from potassium chloride or sodium chloride.

[0009] The liquid carrier includes water and an organic solvent; The water is preferably deionized water; The organic solvent is selected from at least one of aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, aliphatic hydrocarbons, alcohols, ethers, ketones, special solvents, vegetable oils, and methylated vegetable oils; The aromatic hydrocarbon is selected from at least one of benzene, xylene, trimethylbenzene, and toluene; the chlorinated aliphatic hydrocarbon is selected from at least one of chloroform, dichloromethane, chloroform, carbon tetrachloride, and polychlorinated ethanes; the aliphatic hydrocarbon is selected from at least one of petroleum fractions, cyclohexane, light mineral oil, and paraffin; the alcohol is selected from at least one of methanol, ethanol, isopropanol, butanol, isobutanol, ethylene glycol, propylene glycol, glycerol, and fatty alcohols; the ether is selected from at least one of propylene glycol ethyl ether, propylene glycol methyl ether, and petroleum ether; the ketone is selected from acetone, At least one of cyclohexanone, isoflurone, N-methylpyrrolidone, and N-octylpyrrolidone; the special solvent is selected from at least one of dimethylformamide, dimethyl sulfoxide, polyethylene glycol, and acetonitrile; the vegetable oil is selected from at least one of peanut oil, soybean oil, linseed oil, castor oil, and rapeseed oil; the methylated vegetable oil is selected from at least one of methyl oleate, methyl oleate, methyl laurate, methyl stearate, methyl myristate, methyl hexadecanoate, methyl octanoate, methyl caprylate, methyl cocoate, and mixed fatty acid methyl esters; Furthermore, the acaricide composition can be prepared into an agriculturally acceptable formulation, wherein the formulation is a liquid or solid formulation; Furthermore, the solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules; The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsions, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspension agents, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions. Furthermore, the liquid formulation is selected from soluble concentrates, emulsifiable concentrates, water emulsions, microemulsions, dispersible oil suspensions, or suspensions, and the solid formulation is selected from powders, granules, wettable powders, or water-dispersible granules. The present invention also discloses the use of the acaricidal composition described above for the control of plant mites; Furthermore, the plants mentioned are crops, fruit trees, vegetables, flowers, and forest trees, and the mites mentioned are carmine spider mites, citrus rust mites, two-spotted spider mites, truncate spider mites, citrus parsnipus mites, cotton spider mites, or apple parsnipus mites. Furthermore, the harmful mite is the two-spotted spider mite.

[0010] The beneficial effects of this invention are: The acaricide composition of the present invention has a significant synergistic effect on various plant mites. Under the same dosage, it has a better control effect on mites, which can greatly reduce the amount of pesticides used and reduce the production cost of crops. At the same time, it is safe for plants, reduces the risk of increased pesticide resistance in mites, and is safe for the environment. It can be widely used for the integrated control of agricultural mites. Detailed Implementation

[0011] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0012] Formulation preparation examples and methods: Preparation method of water-dispersible granules: According to the formulation ratio of the preparation example, the active ingredient is added to the carrier, and surfactants and other functional additives are added thereto. After mixing, the mixture is pulverized by air jet milling, and 10-25% water is added. Then, the mixture is kneaded, granulated, dried, and sieved to obtain the water-dispersible granule product; or the pulverized powder is sprayed with water, granulated, dried in a fluidized bed granulator, and then sieved to obtain the water-dispersible granules of the present invention.

[0013] Preparation method of water-in-oil emulsion: According to the formulation ratio of the preparation example, the active ingredient is dissolved in the solvent and the emulsifier is added to dissolve it into a homogeneous oil phase. Deionized water and antifreeze are mixed together to form a homogeneous aqueous phase. Under high-speed shearing, the aqueous phase is added to the oil phase to form a well-dispersed water-in-oil emulsion product.

[0014] Emulsifiable concentrate preparation method: According to the formulation ratio of the preparation example, the measured active ingredients, solvents and co-solvents are added to the mixing tank and stirred to dissolve them. Then, the emulsifier is added, and the remaining solvent is used to make up the balance. The mixture is stirred evenly in the mixing tank and filtered to obtain the emulsifiable concentrate of the present invention.

[0015] Microemulsion preparation method: According to the formulation ratio of the preparation example, the active ingredients, solvents, emulsifiers, etc. are mixed evenly to obtain an oil phase. The antifreeze and water are mixed evenly to obtain an aqueous phase. The oil phase is added to the aqueous phase under stirring and stirred evenly. Shearing is continued for 10 minutes. Then, silicone oil defoamer is added and stirred evenly to obtain small droplets with oil phase particles of 0.01~0.1 micrometers, which is the microemulsion of the present invention.

[0016] Preparation method of wettable powder: According to the formulation ratio of the preparation example, the active ingredients, dispersant, wetting agent and filler are mixed and stirred evenly in a stirring tank. The mixture is then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0017] Preparation method of dispersible oil suspension: According to the formulation ratio of the preparation example, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, mixed evenly with oil, subjected to high-speed shearing, wet sand milling, and finally homogenized filtration to obtain the dispersible oil suspension product.

[0018] Suspension preparation method: According to the formulation ratio of the preparation example, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0019]

[0020]

[0021]

[0022] Indoor activity test.

[0023] Example 1: Indoor toxicity determination and screening of compound ratios.

[0024] The activity of compound I against two-spotted spider mites was determined by rationally combining it with other acaricides. Test target: Two-spotted spider mite ( Spider mites Koch) Adult and nymphal mites.

[0025] Test method: Prepare a stock solution of the test drug using an organic solvent, and then prepare five series of concentrations using a 0.1% Tween 80 aqueous solution in equal proportions.

[0026] Mites with consistent physiological states were selected and inoculated onto peanut seedlings with good and relatively uniform growth. 30 mites were inoculated onto each compound leaf. After the mites stabilized, the leaves were cut off, and the leaves were immersed in the test solution for 5 seconds. Excess solution was then absorbed with filter paper, and the leaves were inverted and inserted into a conical flask. The flask was sealed with gauze, with the peanut stem end extending beyond the gauze. The petioles were wrapped with a damp sponge. A control group without the pesticide (containing all organic solvents and emulsifiers) was set up. The treated insects were reared at (25±1)℃ with a photoperiod of L:D = (12:12) h. After 48 hours of pesticide treatment, the mortality of the insects was checked, and the total number of insects and the number of dead insects were recorded.

[0027] Data statistics and analysis: Calculate the mortality rate for each treatment based on the survey data. Use the following formula:

[0028] In the formula: P — Mortality rate, expressed as a percentage (%); K —This indicates the number of dead insects, expressed in heads; N — This indicates the total number of insects treated, in units of heads.

[0029]

[0030] In the formula: P 1 —Adjusted mortality rate, in percentages (%); P t —The mortality rate is expressed as a percentage (%). P 0 — Mortality rate in blank control group, expressed as a percentage (%).

[0031] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0032] The experiment was analyzed using the DPS statistical analysis system to determine the toxicity regression equation, correlation coefficient, and LC. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0033] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0034] In the formula: AT —Measured toxicity index of the mixture; S LC of standard acaricides 50 The unit is milligrams per liter (mg / L); M LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0035]

[0036] In the formula: THIRTY —Theoretical toxicity index of the mixture; YOU A —A. Toxicity index of drug A; P A —The percentage content of drug A in the mixture, expressed as a percentage (%). YOUB —Toxicity index of drug B; P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0037]

[0038] In the formula: CTC —Cotoxicity coefficient; AT —Measured toxicity index of the mixture; THIRTY —Theoretical toxicity index of mixed preparations.

[0039] Co-toxicity coefficient of compound CTC ≥120 exhibits a synergistic effect; CTC≤80 It exhibits antagonistic effects; 80 < CTC <120 exhibits an additive effect.

[0040] Table 1. Results of indoor activity assays of compound I mixed with spirodimethyl diester on adult Tetranychus biloba.

[0041] Table 2. Results of indoor activity assay of compound I mixed with bifenthrin on adult Tetranychus simonii.

[0042] Table 3. Results of indoor activity assays of compound I mixed with fluopyram and adult Tetranychus edulis.

[0043]

[0044] Table 4. Results of indoor activity assay of compound I mixed with cypermethrin on adult Tetranychus simonii.

[0045] Table 5. Results of indoor activity assay of compound I mixed with avermectin on adult Tetranychus simulans.

[0046] Table 6. Results of indoor activity assay of compound I mixed with butyl urea on adult Tetranychus dichotoma.

[0047] Table 7. Results of indoor activity assays of compound I mixed with cypermethrin on adult Tetranychus bicolor.

[0048] Table 8. Results of indoor activity assays of compound I mixed with biphenylhydrazine on adult Tetranychus bisporus.

[0049] Table 9. Results of indoor activity assay of compound I mixed with pyridaben on adult Tetranychus taeniae.

[0050] Table 10 Results of indoor activity assay of compound I mixed with etoxazole on adult Tetranychus simulans.

[0051] Table 11 Results of indoor activity assays of compound I mixed with azoxystrobin on adult Tetranychus simulans.

[0052] Table 12 Results of indoor activity assay of compound I mixed with triazole tin on adult Tetranychus simulans.

[0053] Table 13 Results of indoor activity assay of compound I with specific mixtures of propargite and adult Tetranychus spp.

[0054] Table 14 Results of indoor activity assay of compound I mixed with spirodiclofen against adult Tetranychus spp.

[0055] Table 15 Results of indoor activity assay of compound I mixed with lufenuron on adult Tetranychus bicolor.

[0056] Table 16 Results of indoor activity assay of compound I mixed with fluazinam as an adult two-spotted spider mite

[0057] Table 17 Results of indoor activity assay of compound I mixed with spirotetramat to adult Tetranychus simulans.

[0058] Table 18 Results of indoor activity assay of compound I mixed with dicofol on adult Tetranychus simulans.

[0059] Table 19 Results of indoor activity assay of compound I mixed with lambda-cyhalothrin on adult Tetranychus simonii.

[0060] Table 20 Results of indoor activity assay of compound I mixed with emamectin benzoate on adult Tetranychus simulans.

[0061] Table 21 Results of indoor activity assay of compound I mixed with thiamethoxam on nymphs of Tetranychus biloba.

[0062] Table 22 Results of indoor activity assay of compound I mixed with etoxazole on Tetranychus bicolor nymphs

[0063] Table 23 Results of indoor activity assay of compound I mixed with tetradifon against two-spotted spider mite nymphs

[0064] Table 24 Results of indoor activity assay of compound I mixed with spirodiclofen against Tetranychus bifidum nymphs

[0065] The results of the indoor experiments (Tables 1-24) show that compound I, when rationally mixed with any one of the following, exhibits a synergistic effect on Tetranychus tinctoria, within a certain proportion range: abamectin, spirodiclofen, bifenthrin, cypermethrin, flufenoxuron, etoxazole, bifenazate, azithromycin, buprofen, pyridaben, etoxazole, cyprodinil, emamectin benzoate, propargite, spirodiclofen, diflubenzuron, tetradifon, thiamethoxam, lufenuron, fluazinam, spirotetramat, spirodiclofen, and lambda-cyhalothrin.

[0066] Based on the above experimental methods, the indoor activity of compound I mixed with the listed acaricides on *Tetranychus erythrorhizoma* was also determined. The results showed that compound I exhibited a certain synergistic effect with the listed acaricides within a mass range of 1:40 to 50:1.

[0067] Field efficacy trials.

[0068] Example 2: Field test of different acaricide compositions for the control of apple spider mites.

[0069] Experimental location: The experiment was conducted in Aiqiankuang Village, Songshan Street, Qixia City, Yantai City, Shandong Province. The experimental area was a hilly area with no weeds or other coverings on the ground. The soil was sandy loam. The cultivation conditions of all experimental plots were consistent and in line with local agricultural practices.

[0070] Experimental crop: The experimental crop was an 8-year-old Red Fuji apple, with a plant spacing of 3m×3m. During the experiment, the apples were in the fruit enlargement stage.

[0071] Test target: Apple spider mite.

[0072] Test reagents: The test reagents and control reagents are shown in Table 25. Water was used as a blank control.

[0073] Experimental plot setup: Two fruit trees per plot, randomly assigned to each treatment, with four replicates. No other pesticides were applied during the experiment. Experimental Methods: The experiment involved applying pesticide once at the initial stage of spider mite infestation. A Gongnong 36 high-pressure motorized sprayer was used, with a nozzle diameter of 1.0 mm, operating pressure of 3.5 MPa, and a single-nozzle flow rate of 2.5 L / min. Spraying was required to be even and thorough, ensuring uniform coverage of the entire plant, with all leaves, both inside and outside the canopy, evenly coated and dripping wet.

[0074] Survey time and method: One tree was surveyed in each plot. One lateral branch of each tree was randomly marked in five directions (east, south, west, north, and center). The number of active mites on five leaves of each lateral branch was recorded from top to bottom using a handheld magnifying glass. The number of remaining active mites was surveyed on the 3rd, 7th, and 14th days after the application of the pesticide.

[0075] The prevention and control effect is calculated using the following formula:

[0076] The test results are shown in the table below: Table 25 Results of field trials of different acaricide compositions against apple spider mites

[0077] Example 3: Field test of different acaricide compositions for controlling cotton red spider mites.

[0078] Experimental location: The experiment was conducted at a cotton planting base in Chiping County, Liaocheng City, Shandong Province. The experimental area was alkali land with few weeds. The cultivation and management level was relatively high.

[0079] Experimental crop: cotton (Lu Mian Yan 37).

[0080] Experimental target: cotton red spider mite.

[0081] Experimental design: The experimental and control reagent plots were arranged in a randomized block, with a plot area of ​​20m². 2 Each treatment was repeated four times. The experiment was conducted once during the peak period of cotton spider mite infestation, which coincided with the cotton flowering and boll-forming stage. A Gongnong-16 backpack sprayer was used to apply the pesticide evenly to the entire plant.

[0082] Experimental survey: Each plant in each plot was labeled with 30 leaves for investigation. The efficacy of the control was investigated at 3, 7 and 10 days after the application of the pesticide, and the number of live mites was recorded.

[0083] Direct impact on crops: During the trial period, the effects of the test and control pesticides on crops were observed periodically to determine whether any phytotoxicity occurred.

[0084] The prevention and control effect is calculated using the following formula:

[0085] The test results are shown in the table below: Table 26 Results of field trials on red spider mites on cotton plants using different acaricide compositions.

[0086] Example 4: Field test of different acaricide compositions for the control of red spider mites in citrus.

[0087] Experimental site: The experiment was conducted in a citrus orchard in Caijiayang Village, Nancheng Street, Huangyan District, Taizhou City, Zhejiang Province. The soil fertility of the experimental site was moderate to high, and the drainage and irrigation were good.

[0088] Experimental crop: Citrus (Red Beauty).

[0089] Experimental Design: The experiment consisted of 22 treatments, with water as a blank control. Each treatment was randomly assigned and replicated 4 times, with two citrus trees in each plot.

[0090] Experimental method: On June 12, 2021, the experiment was conducted by applying the pesticide once using the Hongtudi Electric Backpack-16L Backpack Sprayer.

[0091] Survey Methods: The experiment was conducted at 3, 7, and 14 days after pesticide application, and the number of active mites was recorded. During the survey, a representative citrus tree was selected from each plot, and the tender shoots were marked in five directions: east, south, west, north, and center. The number of active mites on a total of 25 leaves was investigated. The leaf surface was directly observed with a handheld magnifying glass, and the number of mites was counted.

[0092] Safety survey: After application of pesticides, the growth of citrus trees and leaves in each area will be investigated periodically to check for any pesticide damage.

[0093] The prevention and control effect is calculated using the following formula:

[0094] The test results are shown in the table below: Table 27 Field control trials of different acaricide compositions against citrus red spider mites

[0095] As shown in Examples 2-4 above, when compound I is combined with various acaricides, it can reduce the dosage of both, improve the control efficacy against mites, and improve the speed of action and prolong the duration of action to varying degrees.

[0096] During the trial, irregular observations were conducted, and no visible phytotoxicity symptoms were found in any of the formulations in the examples on the above-mentioned test crops. The crops grew well after application.

[0097] It should be understood that the above embodiments are merely some embodiments of the present invention, provided only for a better understanding of the embodiments of the present invention, and are not all embodiments of the present invention. In practical applications, by adjusting the content of each component and the composition of the components in the present invention, different and numerous embodiments can be obtained, all of which are within the scope of the present invention.

[0098] The embodiments of the present invention have been described above. However, the present invention is not limited to the above embodiments. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An acaricide composition containing a substituted phenyl sulfide compound, characterized in that, The acaricide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound represented by formula I: (I) The active ingredient B is any one of growth inhibitor acaricides, neurotoxin acaricides, and other acaricides. The growth inhibitor acaricides are: spirodiclofen, cypermethrin, spirodiclofen, lufenuron, spirotetramat, thiamethoxam, etoxazole, tetradifon, and spirodiclofen. The neurotoxin acaricides are bifenthrin, bifenazate, triazophos, lambda-cyhalothrin, and cypermethrin. The other acaricides are propargite. The mass ratio of active ingredient A to active ingredient B is 1:45 to 40:

1.

2. The acaricide composition according to claim 1, characterized in that, The active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:40~20:1, preferably 1:30~20:

1. The active ingredient B is cypermethrin, and the mass ratio of active ingredient A to active ingredient B is 1:35~45:1; The active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:30~45:1; The active ingredient B is lufenuron, and the mass ratio of active ingredient A to active ingredient B is 1:40~45:1; The active ingredient B is spirotetramat, and the mass ratio of active ingredient A to active ingredient B is 1:30 to 30:

1. The active ingredient B is thiamethoxam, and the mass ratio of active ingredient A to active ingredient B is 1:30~35:1; The active ingredient B is etoxazole, and the mass ratio of active ingredient A to active ingredient B is 1:30~32:1; The active ingredient B is tetradifon, and the mass ratio of active ingredient A to active ingredient B is 1:36~35:1; The active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:33~30:1; The active ingredient B is bifenthrin, and the mass ratio of active ingredient A to active ingredient B is 1:40~35:1; The active ingredient B is biphenylhydrazine ester, and the mass ratio of active ingredient A to active ingredient B is 1:30~40:1; The active ingredient B is triazole tin, and the mass ratio of active ingredient A to active ingredient B is 1:30~33:1; The active ingredient B is high-efficiency cyhalothrin, and the mass ratio of active ingredient A to active ingredient B is 1:42~32:1; The active ingredient B is cypermethrin, and the mass ratio of active ingredient A to active ingredient B is 1:35~25:1; The active ingredient B is propargite, and the mass ratio of active ingredient A to active ingredient B is 1:50~50:

1.

3. The acaricide composition according to claim 2, characterized in that, The active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:30~20:1; The active ingredient B is cypermethrin, and the mass ratio of active ingredient A to active ingredient B is 1:15 to 45:

1. The active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:15 to 45:

1. The active ingredient B is lufenuron, and the mass ratio of active ingredient A to active ingredient B is 1:20~30:1; The active ingredient B is spirotetramat, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 20:

1. The active ingredient B is thiamethoxam, and the mass ratio of active ingredient A to active ingredient B is 1:20~25:1; The active ingredient B is etoxazole, and the mass ratio of active ingredient A to active ingredient B is 1:20~24:1; The active ingredient B is tetradifon, and the mass ratio of active ingredient A to active ingredient B is 1:36~25:1; The active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 20:

1. The active ingredient B is bifenthrin, and the mass ratio of active ingredient A to active ingredient B is 1:25~17:1; The active ingredient B is biphenylhydrazine ester, and the mass ratio of active ingredient A to active ingredient B is 1:15 to 40:1; The active ingredient B is triazole tin, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 10:1; The active ingredient B is high-efficiency cyhalothrin, and the mass ratio of active ingredient A to active ingredient B is 1:36~24:1; The active ingredient B is cypermethrin, and the mass ratio of active ingredient A to active ingredient B is 1:20~25:1; The active ingredient B is propargite, and the mass ratio of active ingredient A to active ingredient B is 1:50~30:

1.

4. The acaricide composition according to claim 2, characterized in that, The active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:40, 1:30, 1:20, 1:12, 1:5, 5:7, 1:1, 3:2, 7:1, or 20:

1. The active ingredient B is cypermethrin, and the mass ratio of active ingredient A to active ingredient B is 1:35, 1:15, 2:5, 1:1, 5:2, 25:1, 30:1, or 45:

1. The active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:30, 1:15, 1:6, 1:2, 2:1, 8:1, 20:1, 35:1, or 45:

1. The active ingredient B is lufenuron, and the mass ratio of active ingredient A to active ingredient B is 1:40, 1:20, 1:10, 1:5, 1:1, 5:1, 15:1, 30:1, or 45:

1. The active ingredient B is spirotetramat, and the mass ratio of active ingredient A to active ingredient B is 1:30, 1:20, 1:10, 1:5, 1:1, 5:1, 10:1, 20:1, or 30:

1. The active ingredient B is thiamethoxam, and the mass ratio of active ingredient A to active ingredient B is 1:30, 1:20, 1:10, 1:3, 2:1, 5:1, 10:1, 15:1, 25:1, or 35:

1. The active ingredient B is etoxazole, and the mass ratio of active ingredient A to active ingredient B is 1:30, 1:20, 1:10, 1:3, 3:1, 6:1, 12:1, 24:1, or 32:

1. The active ingredient B is tetradifon, and the mass ratio of active ingredient A to active ingredient B is 1:36, 1:24, 1:12, 1:6, 2:1, 5:1, 10:1, 15:1, 25:1, or 35:

1. The active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:33, 1:20, 1:10, 1:5, 1:1, 5:1, 10:1, 15:1, 20:1, or 30:

1. The active ingredient B is bifenthrin, and the mass ratio of active ingredient A to active ingredient B is 1:40, 1:25, 1:15, 1:8, 1:5, 1:1, 3:1, 8:1, 17:1, or 35:

1. The active ingredient B is biphenylhydrazine ester, and the mass ratio of active ingredient A to active ingredient B is 1:30, 1:15, 1:8, 1:1, 5:1, 10:1, 20:1, 30:1, or 40:

1. The active ingredient B is triazole tin, and the mass ratio of active ingredient A to active ingredient B is 1:30, 1:20, 1:10, 1:5, 1:2, 4:1, 10:1, 22:1, or 33:

1. The active ingredient B is high-efficiency cyhalothrin, and the mass ratio of active ingredient A to active ingredient B is 1:40, 1:36, 1:18, 1:6, 2:5, 3:1, 7:1, 15:1, 24:1, or 32:

1. The active ingredient B is cypermethrin, and the mass ratio of active ingredient A to active ingredient B is 1:35, 1:20, 1:15, 1:7, 2:3, 1:1, 3:2, 8:1, 16:1, or 25:

1. The active ingredient B is propargite, and the mass ratio of active ingredient A to active ingredient B is 1:50, 1:45, 1:30, 1:15, 1:2, 5:1, 15:1, 30:1, or 40:

1.

5. The acaricide composition according to claim 1, characterized in that, The total weight of the acaricide composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 2% to 90% of the total weight of the acaricide composition.

6. The acaricide composition according to claim 5, characterized in that, The total weight of the acaricide composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 2% to 60% of the total weight of the acaricide composition.

7. The acaricide composition according to claim 1, characterized in that, The acaricide composition can be prepared into an agriculturally acceptable formulation, wherein the formulation is a liquid or solid formulation.

8. The acaricide composition according to claim 7, characterized in that, The liquid formulation is selected from soluble concentrates, emulsifiable concentrates, water emulsions, microemulsions, dispersible oil suspensions, or suspensions, and the solid formulation is selected from powders, granules, wettable powders, or water-dispersible granules.

9. Use of the acaricidal composition according to any one of claims 1-8 for the control of plant mites.

10. The use according to claim 9, characterized in that, The plants mentioned are crops, fruit trees, vegetables, flowers, and forest trees, and the mites mentioned are carmine spider mites, citrus rust mites, two-spotted spider mites, truncate spider mites, citrus paronychia mites, cotton spider mites, or apple paronychia mites. Preferably, the harmful mite is the two-spotted spider mite.

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