Use of diphenyl isophthalate in the preparation of an attractant for caterpillars of the black cutworm

Abstract

The application discloses application of diphenyl isophthalate in preparation of an attractant for scirpophaga incertulas larvae. The application finds that diphenyl isophthalate has an attraction effect on scirpophaga incertulas larvae. Therefore, the scirpophaga incertulas larvae can be trapped by using the simple compound to overcome problems such as high cost and difficult operation of other technologies, and diversified attractant options are provided for monitoring and prevention and control of the scirpophaga incertulas larvae. The application has simple and flexible use modes. The single-component attractant for the scirpophaga incertulas larvae, i.e. the diphenyl isophthalate, can be used for monitoring or combined with pesticides for prevention and control of the scirpophaga incertulas larvae, and is convenient to use.

Description

Technical Field

[0001] This invention belongs to the field of pest monitoring and trapping technology, specifically relating to the application of diphenyl isophthalate in the preparation of attractants for cutworm larvae. Background Technology

[0002] Little ground tiger ( Agrotis ipsilon Noctuidae (Lepidoptera) is a species of underground moth with a wide host range, particularly damaging vegetables and tobacco, making it a significant pest. Current control methods for underground pests still rely on chemical pesticides, which pose risks of food and environmental contamination. Green pest control can reduce the use of chemical pesticides, protecting human health and environmental safety.

[0003] Using attractants to trap and kill the small cutworm larvae is a key measure in green pest control. Currently, common ecological trapping techniques for small cutworms mainly involve sex pheromones and food attractants for adults, and sugar-vinegar-alcohol baits for larvae. These techniques all suffer from high costs and poor stability. Therefore, selecting new attractants is necessary for the control of small cutworm larvae.

[0004] Diphenyl isophthalate is used in organic synthesis and also as a plasticizer for nylon resins. Summary of the Invention

[0005] The purpose of this invention is to provide the application of diphenyl isophthalate or its analogues in attracting cutworms, overcoming the shortcomings of existing attractants such as high cost and poor stability, and thus facilitating field application.

[0006] Through screening experiments, this patent discovered that diphenyl isophthalate has an attractive effect on small cutworm larvae. Therefore, this simple compound can be used to trap small cutworm larvae, overcoming the problems of high cost and difficult operation of other technologies, and providing a diversified selection of attractants for the monitoring and control of small cutworm larvae.

[0007] The first object of the present invention is to provide the use of diphenyl isophthalate or its analogues in the preparation of attractants for small cutworms.

[0008] Preferably, the cutworm is a cutworm larva, and more preferably a fifth-instar cutworm larva.

[0009] Preferably, the concentration of diphenyl isophthalate is 0.01-10 mg / mL.

[0010] A second object of the present invention is to provide the use of diphenyl isophthalate or its analogues in the attraction, control and / or monitoring of cutworms.

[0011] Preferably, the application involves applying diphenyl isophthalate or its analogues to a blank lure to attract cutworm larvae; or applying diphenyl isophthalate or its analogues to a blank lure and then using the lure in combination with a chemical pesticide / biological pesticide.

[0012] A third objective of this invention is to provide a method for preventing and / or monitoring cutworm larvae, which involves applying diphenyl isophthalate or its analogues to the habitat of cutworm larvae to attract them.

[0013] Preferably, the concentration of diphenyl isophthalate is 0.01-10 mg / mL.

[0014] Preferably, diphenyl isophthalate or its analogues are applied to a blank lure to attract cutworm larvae; or diphenyl isophthalate or its analogues are applied to a blank lure, and then the lure is used in combination with a chemical pesticide / biological pesticide.

[0015] Compared with the prior art, the advantages of the present invention are:

[0016] The method of use is simple and flexible. The single-component attractant for cutworm larvae provided by this invention—diphenyl isophthalate—can be used for monitoring or in combination with pesticides for the control of cutworm larvae, and is easy to use. Attached Figure Description

[0017] Figure 1 The values ​​represent the response of small cutworm larvae to different concentrations (0.01, 0.1, 1, 5, 10 mg / mL) of diphenyl isophthalate, with liquid paraffin serving as a control (CK).

[0018] Figure 2 This study investigated the selective effect of cutworm larvae on diphenyl isophthalate (DIP). Acetic acid was used as a positive control. Data are expressed as mean ± standard error. Bars marked with an asterisk indicate significant differences between the treatment and control groups (*p<0.01; NSP>0.05) (chi-square test). Different lowercase letters above the bars indicate significant differences (p<0.05). Detailed Implementation

[0019] The present invention will be further described in detail below with reference to specific embodiments. The embodiments given are only for illustrating the present invention and are not intended to limit the scope of the present invention.

[0020] Example 1

[0021] To determine the response of cutworm larvae to diphenyl isophthalate, an electrode potential amplification (EAG) experiment was performed. Antennae were harvested from fifth-instar cutworm larvae 24 hours after molting under a microscope. The intact antennae were mounted on bifurcated electrodes pre-coated with conductive gel. The EAG amplification response of the larval antennae to different concentrations (0.01, 0.1, 1, 5, 10 mg / mL) of diphenyl isophthalate was recorded using EAGPro software (version V2.0; Synstech). The parameters were set as follows: Y amplitude 0.5 mV, time base 3 sec, continuous flow 500 mL / min, stimulation flow 300 mL / min, stimulation duration 0.2 sec, and stimulation interval 180 sec. The test compound was sequentially diluted to the desired concentration in liquid paraffin. A 1 × 1 cm strip of filter paper was inserted into the tip of a 1000 μL pipette. Ten microliters of diphenyl isophthalate were evenly dropped onto a filter paper strip in a "Z" shape and then transferred to a pipette. The loaded strip was then transferred to a pipette. Stimulations were performed in order from lowest to highest concentration. Controls (using liquid paraffin as a reference) were recorded before and after each test sample. For each compound concentration tested, antennae from 30 individual larvae were used as biological replicates.

[0022] Experimental results showed that the antennae of the small cutworm larvae responded to different concentrations (0.01, 0.1, 1, 5, 10 mg / mL) of diphenyl isophthalate, especially to 0.1 mg / mL diphenyl isophthalate, with a relative response value of approximately 0.0002 for the antennal potential during EAG assay. Figure 1 ).

[0023] Example 2

[0024] To confirm the response of cutworm larvae to diphenyl isophthalate, we conducted experiments using a Y-type olfactometer. A 10 μL solution of diluted diphenyl isophthalate (0.01–10 mg / mL) or a solvent control (liquid paraffin) was evenly applied to a filter paper strip (1 cm × 1 cm), which was then placed in an odor source bottle. A fifth-instar cutworm larva, 24 hours post-molting, was introduced into the main arm of the Y-type olfactometer (main arm: 20 cm long × 6 cm inner diameter; two branch arms: 8 cm long × 3 cm inner diameter), and its behavior was observed within the apparatus for 3 minutes. Selective behavior was recorded if the larva crossed the midpoint of a branch arm and remained there for at least 30 seconds. To control for positional bias, the left and right arms of the olfactometer were replaced after every 5 larvae were tested, and the entire olfactometer was replaced after every 10 larvae were tested. Furthermore, the positions of the sample and control odor sources were exchanged after every 20 larvae were tested. To eliminate residual odor contamination, the Y-type olfactometer and odor source bottle were rinsed three times with 100% ethanol after each use and baked at 200°C for 2 hours. The connected silicone tubing was also baked at 70°C for 2 hours. All experiments were conducted in a constant-temperature room under natural sunlight conditions at a controlled temperature of 25 ± 1°C. The selection rate was calculated as follows: Selection rate (%) = (Number of larvae selected from a particular arm / Total number of larvae tested) × 100. Liquid paraffin and acetic acid were used as control stimuli. Each larva was used only once, therefore each treatment had 30 biological replicates.

[0025] The test results are shown in Figure 2 Attraction rate (%) = (Number of attracted heads / Total number of tested heads) x 100. Results showed that cutworm larvae were strongly attracted to diphenyl isophthalate (0.1 mg / mL). Acetic acid was used as a positive control. Data are presented as mean ± standard error. An asterisked bar indicates a significant difference between the treatment and control groups (*p<0.01; NSP>0.05) (chi-square test). Different lowercase letters above the bars indicate significant differences (p<0.05).

Claims

1. Application of diphenyl isophthalate or its analogues in the preparation of attractants for small cutworms.

2. The application according to claim 1, characterized in that, The cutworm in question is a cutworm larva, preferably a fifth-instar cutworm larva.

3. The application according to claim 1 or 2, characterized in that, The concentration of diphenyl isophthalate is 0.01-10 mg / mL.

4. Application of diphenyl isophthalate or its analogues in the attraction, control and / or monitoring of cutworms.

5. The application according to claim 4, characterized in that, The application involves applying diphenyl isophthalate or its analogues to a blank lure to attract cutworm larvae; or applying diphenyl isophthalate or its analogues to a blank lure and then using the lure in combination with a chemical pesticide / biological pesticide.

6. The application according to claim 4, characterized in that, The cutworm in question is a cutworm larva, preferably a fifth-instar cutworm larva.

7. The application according to claim 4, characterized in that, The concentration of diphenyl isophthalate is 0.01-10 mg / mL.

8. A method for controlling and / or monitoring cutworm larvae, characterized in that, This involves applying diphenyl isophthalate or its analogues to the habitat of small cutworm larvae to attract them.

9. The method according to claim 8, characterized in that, The concentration of diphenyl isophthalate used is 0.01-10 mg / mL.

10. The application according to claim 8 or 9, characterized in that, This involves applying diphenyl isophthalate or its analogues to a blank lure to attract cutworm larvae; or applying diphenyl isophthalate or its analogues to a blank lure and then using the lure in combination with a chemical pesticide / biological pesticide.

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