Non-trpm8-activating cooling e-liquid and preparation method thereof

Abstract

The application provides a non-TRPM8 activation type cool taste electronic atomization liquid and a preparation method. The non-TRPM8 activation type cool taste electronic atomization liquid comprises the following components in parts by weight: a base solvent: 40-120 parts, a core functional compound 1-15 parts, an antioxidant 0.05-0.5 parts, a nicotine salt 0-5 parts and an acidity regulator. The base solvent comprises propylene glycol and plant glycerol. The acidity regulator is used to adjust the pH value of the electronic atomization liquid to 5.5-7.0. The core functional compound is compounded by plant extracts and functional esters at a weight ratio of 1:0.2-1:5. The application has the beneficial effect that the cool feeling is realized through the non-TRPM8 activation mechanism, the cool feeling is more stable and persistent, and the smoothness of the taste is more outstanding.

Description

Technical Field

[0001] This invention relates to the field of e-cigarette liquid technology, specifically to a non-TRPM8 activated cool-flavor e-cigarette liquid suitable for e-cigarettes and its preparation method. Background Technology

[0002] The cooling sensation in e-cigarette liquids has long relied on cooling agents such as the WS series (e.g., WS-3, WS-5, WS-23) and menthol. These substances produce a cooling sensation by specifically activating the TRPM8 cold receptor in the human body, and have become the mainstream technology in the industry. However, with the continued tightening of global e-cigarette regulations, markets such as Germany and the European Union have explicitly restricted or banned the use of such TRPM8 receptor agonists, causing significant compliance uncertainty for traditional technologies based on this mechanism.

[0003] In existing technologies, some solutions improve the taste by compounding multiple cooling agents, but they still do not deviate from the technical path of TRPM8 activation and cannot meet the requirements of the new regulations. Other solutions attempt to provide a cooling sensation by using plant extracts, but there are problems such as insufficient cooling intensity, poor persistence, and poor compatibility with PG / VG base liquid. In addition, they are prone to bitterness or irritation, making it difficult to balance the cooling experience with the smoothness of the taste.

[0004] Therefore, the industry urgently needs to develop a new type of cooling e-liquid that does not rely on TRPM8 receptor activation for its cooling sensation, completely avoids the current and anticipated list of banned cooling agents, and can achieve a stable and reproducible cooling experience while also providing a smooth and comfortable inhalation sensation. Summary of the Invention

[0005] To address the compliance risks associated with adding cooling agents in existing technologies, as well as the insufficient cooling sensation and strong taste irritation of plant extract solutions, this invention provides a non-TRPM8 activated cooling electronic atomizing liquid and its preparation method, achieving a compliant, refreshing, smooth, and stable atomization experience.

[0006] The non-TRPM8 activated cooling e-cigarette liquid of this invention comprises the following components in the following weight ratio: Base solvent: 40-120 parts, core functional compound: 1-15 parts, antioxidant: 0.05-0.5 parts, nicotine salt: 0-5 parts, and acidity regulator. The base solvent includes propylene glycol and vegetable glycerin. The acidity regulator is used to adjust the pH value of the electronic atomizing liquid to 5.5-7.0. The core functional compound is composed of a combination of plant extracts and functional esters in a weight ratio of 1:0.2-1:5.

[0007] Furthermore, the combination of plant extracts includes multiple or all of eucalyptus leaf extract, spearmint extract, chamomile extract, and green tea extract.

[0008] Furthermore, the plant extract combination comprises the following by weight percentage: 43%-53% eucalyptus extract, 23%-33% spearmint extract, 8%-14% chamomile extract, and 6%-12% green tea extract.

[0009] Furthermore, the eucalyptus extract contains ≥85% 1,8-cineole, and the spearmint extract contains ≥90% carvacrol.

[0010] Furthermore, the functional esters include one or more of propylene glycol diacetate, triacetylglycerol, and isoamyl acetate.

[0011] Furthermore, the antioxidant includes at least one of rosmarinic acid, vitamin E, and tea polyphenols.

[0012] Furthermore, the acidity regulator includes at least one of trisodium citrate, malic acid, and tartaric acid.

[0013] Furthermore, the weight ratio of propylene glycol to vegetable glycerin in the base solvent is 1:0.5-1:3.

[0014] This invention also provides a method for preparing an electronic atomizing fluid, used to prepare the aforementioned non-TRPM8 activated cooling electronic atomizing fluid, comprising the following steps: S1: Extract pretreatment: Mix the components of the plant extract combination in proportion, add 5%-10% propylene glycol, and ultrasonically disperse for a first set time to obtain a plant extract premix. S2: Ester compound: Mix the functional ester components in proportion, add them to the plant extract premix, stir for a second set time to obtain the core functional compound; S3: Base solution preparation: Mix the remaining propylene glycol and vegetable glycerin in a certain proportion, add antioxidants and acidity regulators, stir until dissolved, and obtain the base solution; S4. Mixing and Curing: Add the core functional compound to the base liquid, then add nicotine salt, stir evenly, and then seal and cure under the set curing temperature and light-proof conditions to obtain the final electronic atomizing liquid.

[0015] Furthermore, in step S4, the maturation temperature is 25-30℃, the maturation time is 48-72 hours, and the mixture is stirred once every 12 hours during the maturation period.

[0016] Compared with the prior art, the beneficial effects of the present invention are: 1. The product of the present invention can achieve compliance: The composition of the present invention is completely free of prohibited cooling agents such as WS-23 and menthol, and achieves a cooling sensation through a non-TRPM8 activation mechanism, which fully complies with the proposed German regulations and relevant EU compliance requirements. 2. The present invention can achieve a more stable and lasting cooling sensation: The present invention achieves a cooling sensation intensity similar to that of the traditional menthol solution by combining the refreshing taste of plant extracts with the volatile carrying effect of functional esters, and the duration is extended by more than 30%. The overall cooling sensation is natural and without irritation or abruptness. 3. The invention has an outstanding smooth taste: The polyphenols in chamomile and green tea extracts can significantly reduce the irritation of aerosols to the respiratory tract. At the same time, the functional esters can optimize the surface tension of the PG / VG base solvent and reduce the formation of condensate. Combined with the pH adjustment effect of the acidity regulator, the bitterness and irritation of traditional plant-based solutions are completely solved, resulting in a dense and smooth atomized taste. 4. Good compatibility and stability: The basic solvent of this invention includes propylene glycol (PG) and vegetable glycerin (VG) to form a PG / VG base liquid. The core functional compound has excellent synergistic compatibility with the PG / VG base liquid. After high-temperature accelerated aging treatment and low-temperature freezing treatment, the composition does not separate or precipitate. The retention rate of cooling and smoothness is ≥95%, and the stability is better, which can meet the requirements of long-term storage and transportation of products. Detailed Implementation

[0017] Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains; the terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to limit the invention; the terms "comprising" and "having," and any variations thereof, in the specification and claims are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the specification and claims are used to distinguish different objects, not to describe a particular order.

[0018] In this invention, the reference to "embodiment" means that a specific feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of the invention. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor is it a mutually exclusive, independent, or alternative embodiment to other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described in this invention can be combined with other embodiments.

[0019] To enable those skilled in the art to better understand the present invention, the technical solution of the present invention will be clearly and completely described below in conjunction with embodiments.

[0020] This invention relates to a non-TRPM8 activated cooling e-liquid, comprising the following components by weight: a base solvent of 40-120 parts, a core functional compound of 1-15 parts, an antioxidant of 0.05-0.5 parts, nicotine salt of 0-5 parts, and an acidity regulator. The base solvent comprises propylene glycol and vegetable glycerin. The acidity regulator is used to adjust the pH of the e-liquid to 5.5-7.0. The core functional compound is composed of a combination of plant extracts and functional esters in a weight ratio of 1:0.2-1:5. In this example, nicotine salt is optional and can be added or omitted.

[0021] The composition of this invention is completely free of prohibited cooling agents such as WS-23 and menthol, and achieves a cooling sensation through a non-TRPM8 activation mechanism, fully complying with proposed German regulations and relevant EU compliance requirements. Furthermore, this invention achieves a technical effect comparable to the cooling intensity of traditional menthol solutions through the refreshing taste of the combination of plant extracts and the volatile carrying effect of functional esters.

[0022] This example of a combination of plant extracts includes one or more of eucalyptus leaf extract, spearmint extract, chamomile extract, and green tea extract.

[0023] Preferably, the plant extract combination in this example comprises the following by weight percentage: 43%-53% eucalyptus extract, 23%-33% spearmint extract, 8%-14% chamomile extract, and 6%-12% green tea extract.

[0024] This invention, through the formulation of the above four components, can achieve a cooling sensation similar to that of existing technologies and a better taste. Specifically, by using 1,8-cineole from eucalyptus extract to increase the aerosol evaporation rate and reduce the oral surface temperature, a physical cooling effect is achieved. This effect is synergistic with the refreshing taste of spearmint extract and the volatile carrying effect of functional esters. The resulting cooling sensation intensity can reach 80%-90% of that of traditional menthol solutions, and the duration is extended by more than 30%. The overall cooling sensation transitions naturally without irritation or abrupt changes.

[0025] This invention utilizes polyphenols from chamomile extract and green tea extract to significantly reduce the irritation of aerosols to the respiratory tract. Functional esters can optimize the surface tension of PG / VG base liquid and reduce condensation formation. Combined with the pH adjustment effect of acidity regulators, it completely solves the bitterness and irritation problems of traditional plant-based solutions, resulting in a smooth and creamy atomized taste.

[0026] Preferably, the eucalyptus extract contains ≥85% 1,8-cineole and the spearmint extract contains ≥90% carvacrol, resulting in a better cooling effect and a better taste.

[0027] Preferably, the functional ester can be one or more of propylene glycol diacetate, triacetin, and isoamyl acetate. In this example, the antioxidant can be one or more of rosmarinic acid, vitamin E, and tea polyphenols, and the acidity regulator can be one or more of trisodium citrate, malic acid, and tartaric acid.

[0028] Preferably, in this example, the weight ratio of propylene glycol to vegetable glycerin in the base solvent is 1:0.5-1:3. The base solvent of this invention is formed by propylene glycol PG (hereinafter referred to as PG) and vegetable glycerin VG (hereinafter referred to as VG) to form a PG / VG base liquid, which makes the synergistic compatibility between the core functional compound of this invention and the PG / VG base liquid better. After high temperature accelerated aging treatment and low temperature freezing treatment, the composition has no layering or precipitation, and the retention rate of cooling and smoothness is ≥95%, with better stability, which can meet the requirements of long-term storage and transportation of products.

[0029] This invention also provides a method for preparing an electronic atomizing fluid, used to prepare the aforementioned non-TRPM8 activated cooling electronic atomizing fluid, comprising the following steps: S1: Extract pretreatment: Mix the components of the plant extract combination in proportion, add 5%-10% propylene glycol, and ultrasonically disperse for a first set time to obtain a plant extract premix. S2: Ester compound: Mix the functional ester components in proportion, add them to the plant extract premix, stir for a second set time to obtain the core functional compound; S3: Base solution preparation: Mix the remaining propylene glycol and vegetable glycerin in a certain proportion, add antioxidants and acidity regulators, stir until dissolved, and obtain the base solution; S4. Mixing and Curing: Add the core functional compound to the base liquid, then add nicotine salt, stir evenly, and then seal and cure under the set curing temperature and light-proof conditions to obtain the final electronic atomizing liquid.

[0030] This invention pre-treats the plant extract combination to fully dissolve it in propylene glycol, forming a well-dispersed solution, and then combines it with functional lipids. This allows the plant extract combination and functional lipids to fully integrate, greatly enhancing the synergistic effect of the two in terms of taste.

[0031] Preferably, in step S4, the curing temperature is 25-30℃, the curing time is 48-72 hours, and the mixture is stirred once every 12 hours.

[0032] The non-TRPM8 activated cooling e-liquid in this embodiment has the following components by weight: 1. Base solvents: 60 parts PG, 60 parts VG; 2. Core functional compound: 1 part, wherein the plant extract combination includes 50% eucalyptus leaf extract, 30% spearmint extract, 8% chamomile extract, and 12% green tea extract, compounded with functional esters at a mass ratio of 1:2. The functional esters include propylene glycol diacetate and triacetylglycerol, with a propylene glycol diacetate:triacetylglycerol ratio of 1:2. In this example, the plant extract combination is 7.2g, propylene glycol diacetate is 4.8g, and triacetylglycerol is 9.6g.

[0033] 3. 0.1 parts of rosmarinic acid; 4. 0.05 parts of trisodium citrate; 5. Nicotine salt 2.2 parts.

[0034] The preparation method of the electronic atomizing fluid in this embodiment is as follows: S1. Take eucalyptus leaf extract, spearmint extract, chamomile extract and green tea extract in their respective proportions, add 3 parts PG, and sonicate at 500W for 15 minutes to obtain a plant extract premix. S2. Take 4.8g of propylene glycol diacetate and 9.6g of triacetylglycerol, stir evenly, add the above plant extract premix, stir for 40 minutes to obtain the core functional compound; S3. Take the remaining PG and VG, add rosmarinic acid and trisodium citrate, stir until dissolved, and obtain the base solution; S4. Add the core functional compound and nicotine salt to the base liquid, stir evenly, and then cure in a sealed container at 28°C in the dark for 60 hours, stirring once every 12 hours during the process, to obtain electronic atomizing liquid 1.

[0035] The non-TRPM8 activated cooling e-liquid in this embodiment has the following components by weight: 1. Base solvent: 20 parts PG, 60 parts VG; 2. Core functional compound: 8 parts, of which the plant extract combination includes 53% eucalyptus leaf extract, 25% spearmint extract, 14% chamomile extract, and 8% green tea extract, compounded with triacetyl ester at a mass ratio of 1:3; 3. Vitamin E 0.1 part; 4. Malic acid 0.08 parts; 4. Nicotine salt 0 parts.

[0036] The preparation method of the electronic atomizing fluid in this embodiment is as follows: S1. Take eucalyptus leaf extract, spearmint extract, chamomile extract and green tea extract in their respective proportions, add 2 parts PG, and sonicate at 500W for 15 minutes to obtain a plant extract premix. S2. Take the weighed triacetylglycerol, stir it evenly, add it to the above premixed solution, stir for 40 minutes to obtain the core functional compound; S3. Take the remaining PG and VG, add 0.1 parts of vitamin E and 0.08 parts of malic acid, stir until dissolved, and obtain the base solution; S4. Add the core functional compound to the base liquid, stir evenly, and then cure in a sealed container at 28°C in the dark for 48 hours, stirring once every 12 hours during the process, to obtain electronic atomizing liquid 2.

[0037] The non-TRPM8 activated cooling e-liquid in this embodiment has the following components by weight: 1. Base solvent: PG 26.5 parts, VG 13.5 parts; 2. Core functional compound: 15 parts, wherein the plant extract combination includes 43% eucalyptus leaf extract, 33% spearmint extract, 14% chamomile extract, and 10% green tea extract, compounded with propylene glycol diacetate at a mass ratio of 1:1; 3. 0.1 parts of tea polyphenols; 4. Tartaric acid 0.1 parts; 4. Nicotine salt, 4 parts.

[0038] The preparation method of the electronic atomizing fluid in this embodiment is as follows: S1. Take eucalyptus leaf extract, spearmint extract, chamomile extract and green tea extract in their respective proportions, add 2 parts of PG, and sonicate at 500W for 15 minutes to obtain a plant extract premix. S2. Take the weighed propylene glycol diacetate, stir it evenly, add it to the above premixed solution, stir for 40 minutes to obtain the core functional compound; S3. Take the remaining PG and VG, add tea polyphenols and tartaric acid, stir until dissolved, and obtain the base solution; S4. Add the core functional compound and 4.0g of free nicotine salt to the base liquid, stir evenly, and then cure in a sealed container at 28°C in the dark for 72 hours, stirring once every 12 hours during the process, to obtain electronic atomizing liquid 3.

[0039] The traditional e-liquid containing 0.8% menthol is a typical TRPM8 agonist in this case. It produces a strong cooling sensation and refreshing stimulation by directly activating cold receptors in the mouth and throat.

[0040] This test used a traditional e-cigarette liquid containing 0.8% menthol as a comparative example to systematically compare and verify the three non-TRPM8 activated cooling e-cigarette liquids from Examples 1-3 across four dimensions: bioactivity, sensory experience, physicochemical stability, and compliance. The results fully demonstrate that this invention has significant advantages in terms of compliance, taste experience, and long-term stability.

[0041] 1. Testing method: (1) TRPM8 activation rate test.

[0042] Using an in vitro cell receptor activity assay, the nebulized liquids of Examples 1-3 and the comparative example were applied to cell models expressing human TRPM8 cold receptors. The receptor activation level was detected by fluorescence intensity to quantitatively evaluate the degree of stimulation of the cold receptors by the product.

[0043] (2) Sensory evaluation.

[0044] Ten professional sensory evaluators were organized to conduct a blind evaluation and score the intensity of the cooling sensation, the duration of the cooling sensation, and the smoothness of the taste of the atomized liquid upon entry into the mouth in a standardized oral evaluation environment, and the average value was taken.

[0045] (3) Storage stability test.

[0046] The examples and comparative examples were placed in a high-temperature environment of 60°C for 7 days and a low-temperature environment of -20°C for 7 days, respectively, and the appearance changes were observed to evaluate the stability of the system.

[0047] (4) Compliance testing.

[0048] High-performance liquid chromatography (HPLC) and component qualitative detection methods were used to comprehensively screen the cooling agent components in the product to confirm whether it contains prohibited substances such as WS-23 and menthol.

[0049] The performance of the products in the above embodiments and the conventional menthol comparative (containing 0.8% menthol) were tested. The sensory evaluation table is shown in Table 1, and the performance test results are shown in Table 2.

[0050] Table 1 Sensory Evaluation Table for Examples 1-3 and Comparative Examples Table 2 Performance test results of Examples 1-3 and comparative examples 2. Analysis of experimental results: Based on the above experimental results, we can conclude that: (1) According to the sensory tasting results of Examples 1-3 and the comparative examples, the physical cooling of eucalyptus extract, the synergistic taste of spearmint extract, and the evaporation assistance of functional esters achieve a mild cooling sensation close to that of traditional menthol, without any irritation or abruptness; the esters delay the evaporation of the active ingredients, making the cooling sensation smooth and lasting; at the same time, the synergistic optimization of polyphenols and esters reduces aerosol irritation and condensation, completely solving the bitterness and smoothness problems of traditional plant-based atomized liquids; (2) All three embodiments of the present invention achieve a TRPM8 activation rate of <5%, which is far below the industry's conventional threshold, completely breaking away from the technical logic of banning cooling agents, meeting the stringent regulatory requirements of the EU, Germany and other countries, and solving the product compliance pain point from the root. (3) All three embodiments of the present invention showed no change in quality after high and low temperature extreme tests. The core functional compound and PG / VG base liquid have excellent synergy. After accelerated aging at 60℃ for 7 days and freezing at -20℃ for 7 days, the quality retention rate is ≥95%, which fully meets the stability requirements for industrial production, storage and transportation. (4) All three products tested were found to be free of WS-23, menthol, and other prohibited cooling agents. The component testing fully complied with the German legislation to prohibit the use of synthetic and natural cooling agents, and also met the compliance standards of mainstream markets such as the European TPD.

[0051] The specific embodiments described above are preferred embodiments of the present invention and are not intended to limit the specific scope of the present invention. The scope of the present invention includes, but is not limited to, these specific embodiments. All equivalent changes made in accordance with the present invention are within the protection scope of the present invention.

Claims

1. A non-TRPM8 activated cooling electronic atomizing fluid, characterized in that, Includes the following components in parts by weight: Base solvent: 40-120 parts, core functional compound: 1-15 parts, antioxidant: 0.05-0.5 parts, nicotine salt: 0-5 parts, and acidity regulator. The base solvent includes propylene glycol and vegetable glycerin. The acidity regulator is used to adjust the pH value of the electronic atomizing liquid to 5.5-7.

0. The core functional compound is composed of a combination of plant extracts and functional esters in a weight ratio of 1:0.2-1:

5.

2. The non-TRPM8 activated cooling electronic atomizing fluid according to claim 1, characterized in that: The combination of plant extracts includes one or more of eucalyptus extract, spearmint extract, chamomile extract, and green tea extract.

3. The non-TRPM8 activated cooling e-cigarette fluid according to claim 2, characterized in that: The plant extract combination comprises the following components by weight percentage: Eucalyptus extract 43%-53%, spearmint extract 23%-33%, chamomile extract 8%-14%, green tea extract 6%-12%.

4. The non-TRPM8 activated cooling e-cigarette fluid according to claim 3, characterized in that: The eucalyptus extract contains ≥85% 1,8-cineole, and the spearmint extract contains ≥90% carvacrol.

5. The non-TRPM8 activated cooling e-cigarette fluid according to claim 1, characterized in that: The functional esters include one or more of propylene glycol diacetate, triacetylglycerol, and isoamyl acetate.

6. The non-TRPM8 activated cooling e-cigarette fluid according to claim 1, characterized in that: The antioxidants include at least one of rosmarinic acid, vitamin E, and tea polyphenols.

7. The non-TRPM8 activated cooling e-cigarette fluid according to claim 1, characterized in that: The acidity regulator includes at least one of trisodium citrate, malic acid, and tartaric acid.

8. The non-TRPM8 activated cooling electronic atomizing fluid according to claim 1, characterized in that: The weight ratio of propylene glycol to vegetable glycerin in the base solvent is 1:0.5-1:

3.

9. A method for preparing an electronic atomizing fluid, used to prepare the non-TRPM8 activated cooling electronic atomizing fluid according to any one of claims 1-7, characterized in that, Includes the following steps: S1: Extract pretreatment: Mix the components of the plant extract combination in proportion, add 5%-10% propylene glycol, and ultrasonically disperse for a first set time to obtain a plant extract premix. S2: Ester compound: Mix the functional ester components in proportion, add them to the plant extract premix, stir for a second set time to obtain the core functional compound; S3: Base solution preparation: Mix the remaining propylene glycol and vegetable glycerin in a certain proportion, add antioxidants and acidity regulators, stir until dissolved, and obtain the base solution; S4. Mixing and Curing: Add the core functional compound to the base liquid, then add nicotine salt, stir evenly, and then seal and cure under the set curing temperature and light-proof conditions to obtain the final electronic atomizing liquid.

10. The method for preparing the electronic atomizing fluid according to claim 9, characterized in that: In step S4, the maturation temperature is 25-30℃ and the maturation time is 48-72 hours, with stirring once every 12 hours during the process.

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