A perfusion cannula device for a rodent laboratory animal

Abstract

The present application relates to the technical field of experimental animal perfusion experiment apparatus, and particularly relates to a rodent experimental animal perfusion cannula device. The mounting seat, the mounting arm and the adjusting arm of the device constitute a rigid support device, which can provide sufficient and stable support when the sliding plate, the first sliding piece and the second sliding piece move. Meanwhile, the relative position relationship between the clamping part and the experimental rat can be adjusted in multiple dimensions through the sliding cooperation between the sliding plate, the first sliding piece and the second sliding piece. Meanwhile, the puncture needle is smoothly inserted into the target blood vessel through the relative stable sliding between the sliding plate, the first sliding piece and the second sliding piece, which eliminates the inevitable shaking during manual operation, effectively prevents the needle tip from contacting or scratching the inner wall of the blood vessel, and is beneficial to the repeated performance of experimental operation and reduces the requirement for the operation skill of the operator.

Description

Technical Field

[0001] This invention relates to the field of instruments for laboratory animal perfusion experiments, and more particularly to a cannulation device for rodent laboratory animals undergoing perfusion. Background Technology

[0002] In vitro perfusion technology in rodent experiments is a pharmacological and physiological experimental model and an indispensable technique in biomedical research. For example, the rat liver perfusion (IPRL) model is widely used in pharmacokinetics, hepatotoxicity assessment, exploration of ischemia-reperfusion injury mechanisms, and the development of organ transplant preservation solutions due to the high homology between rat liver anatomy and human liver, controllable cost, and pure genetic background. In vitro perfusion technology requires cannulation of the arteries and veins in the experimental animals. For example, portal vein cannulation is necessary in rat liver ex vivo perfusion. The stability of the portal vein cannulation directly determines whether the perfusion fluid can enter the hepatic sinusoids uniformly and continuously, thus affecting the efficiency of oxygen and nutrient exchange. Simultaneously, the inferior vena cava, as the outflow channel, requires real-time management of its outflow fluid for maintaining the cleanliness of the surgical area, preventing cross-contamination, and accurately measuring metabolites.

[0003] However, despite the widespread application of IPRL technology, the engineering level of its experimental setup lags behind. For a long time, researchers have relied on manual operation and simple tools such as sutures and adhesive tape for ligation and fixation when performing arterial and venous cannulation for perfusion. This "workshop" style of operation reveals many limitations when facing the demands of modern high-throughput, long-duration experiments (such as 24-hour ambient temperature machine perfusion): purely manual cannulation methods are highly susceptible to needle puncture of the fragile vascular endothelium, leading to thrombosis or vascular dissection. Summary of the Invention

[0004] To address the aforementioned issues, this application discloses a rodent experimental animal perfusion cannulation device. The device's mounting base, mounting arm, and adjusting arm constitute a rigid support structure, providing sufficient and stable support when the sliding plate, first sliding member, and second sliding member move. Simultaneously, through the sliding cooperation between the sliding plate, first sliding member, and second sliding member, the relative positional relationship between the clamping part and the experimental rat can be adjusted in multiple dimensions. Furthermore, the relatively stable sliding between the sliding plate, first sliding member, and second sliding member ensures the smooth insertion of the puncture needle into the target blood vessel, eliminating the unavoidable shaking during manual operation, effectively preventing the needle tip from contacting or scratching the blood vessel wall, facilitating the repetition of experimental procedures, and reducing the skill requirements for the operator.

[0005] To achieve the above objectives, this application adopts the following technical solution, which includes: Mounting base; The mounting arm is located on the top end face of the mounting base and has a mounting portion facing away from the mounting base; The adjustment unit includes: An adjusting arm is connected to the mounting portion. A sliding plate is provided on one side of the adjusting arm, and the sliding plate is movable along the extending direction of the adjusting arm to move closer to or further away from the mounting arm. A first sliding member is disposed on the side of the sliding plate opposite to the adjusting arm, and is capable of sliding relative to the sliding plate along a first direction; and The second slider is located on the side of the first slider opposite to the sliding plate, and is capable of sliding relative to the first slider in a second direction. Wherein, both the first direction and the second direction are perpendicular to the extension direction of the adjusting arm, and the second direction is perpendicular to the first direction; The intubation unit includes a rotating part and a clamping part. The rotating part is rotatably connected to the second sliding member on the side opposite to the first sliding member. The rotating part has a rotating shaft parallel to the second direction. The clamping part is connected to the rotating part and is used to clamp the irrigation device.

[0006] In one illustrative embodiment of a rodent laboratory animal perfusion cannulation device, the adjustment unit further includes a first drive rod; A limiting groove is provided on one side of the adjusting arm, and a sliding protrusion is provided on the side of the sliding plate facing the adjusting arm. The sliding protrusion is slidably engaged in the limiting groove, and a threaded hole is provided on the sliding protrusion. The first drive rod is rotatably mounted on the adjusting arm, and the first drive rod passes through the limiting slide groove from the end of the adjusting arm away from the mounting arm, and is threadedly engaged with the threaded hole, so that when the first drive rod is rotated, the sliding plate is driven to move along the extension direction of the adjusting arm.

[0007] In one illustrative embodiment of a rodent laboratory animal perfusion cannulation device, one of the sliding plate and the first sliding member is provided with a first slide rail, and the other is provided with a first slide groove. The first sliding member is connected to the sliding plate through the first slide groove and the first slide rail. The sliding plate has a first threaded groove on the side away from the adjusting support arm, and the first sliding member has a first clearance groove on the side facing the sliding plate. The adjustment unit further includes: a second drive rod, which is disposed between the first threaded groove and the first clearance groove, and the second drive rod is rotatably connected to the first sliding member; The second drive rod is also threadedly engaged with the first threaded groove, and when the second drive rod rotates relative to the first sliding member, it can drive the first sliding member to move in the first direction.

[0008] In one illustrative embodiment of a rodent laboratory animal perfusion cannulation device, the first sliding member has a connecting ring at one end in the first direction, the connecting ring being concentric with the first clearance groove, and the second driving rod passing through the connecting ring and being rotatably connected to the connecting ring to restrict the axial movement of the second driving rod relative to the first sliding member.

[0009] In one illustrative embodiment of a rodent laboratory animal perfusion cannulation device, one of the first sliding member and the second sliding member is provided with a second slide rail, and the other is provided with a second slide groove. The second sliding member is connected to the first sliding member through the second slide groove and the second slide rail. The first sliding member has a second threaded groove on the side facing the second sliding member, and the second sliding member has a second clearance groove on the side facing the first sliding member. The adjustment unit further includes: a third drive rod, which is disposed between the second threaded groove and the second threaded groove, and is rotatably connected to the second sliding member; The third drive rod is also threadedly engaged with the second threaded groove, and when the third drive rod rotates relative to the second sliding member, it can drive the second sliding member to move in the second direction.

[0010] In one illustrative embodiment of a rodent laboratory animal perfusion cannulation device, the rotating part includes: A rotating base is fixed to the side of the second sliding member away from the first sliding member. The rotating base has a spherical mounting body. A spherical mounting groove is formed inside the spherical mounting body, and an opening groove communicating with the spherical mounting groove is opened on its outer surface. The opening groove extends circumferentially around the rotating axis. A rotating component includes a ball joint and a connecting shaft. The ball joint is housed in the spherical mounting groove, and the connecting shaft extends from the opening groove and is tangent to the two side walls of the opening groove, thereby restricting the rotating component to swing only around the rotating shaft. The clamping part is connected to the end of the connecting shaft opposite to the ball joint.

[0011] In one illustrative embodiment of a rodent laboratory animal perfusion cannulation device, the rotating part further includes a bolt, wherein the outer surface of the spherical mounting body is provided with a threaded hole, the bolt is screwed into the spherical mounting groove through the threaded hole, and the end of the bolt abuts against the outer surface of the ball joint to lock the rotating part.

[0012] In one illustrative embodiment of a rodent laboratory animal perfusion cannulation device, the clamping part includes: a pair of clamping arms and an elastic member, the pair of clamping arms being rotatably connected, each clamping arm having a clamping end and a force-receiving end, the force-receiving end of one of the clamping arms being detachably connected to the connecting shaft, the elastic member being disposed between the pair of clamping arms and abutting against the pair of clamping arms respectively, providing elastic force to the clamping arms so that the clamping ends of the pair of clamping arms are always abutting against each other.

[0013] In one illustrative embodiment of a rodent laboratory animal perfusion cannulation device, the rodent laboratory animal perfusion cannulation device further includes: An irrigation treatment platform includes a support frame and a drainage grid, wherein the support frame has an installation groove and the drainage grid is disposed in the installation groove.

[0014] In one illustrative embodiment of a rodent laboratory animal perfusion cannulation device, the perfusion treatment platform further includes: A waste liquid discharge unit includes a liquid collection component and a liquid discharge pipe. In the thickness direction of the support frame, the liquid collection component is located below the support frame. The liquid collection component has a guide channel, and the liquid discharge pipe is connected to the guide channel to discharge the liquid in the guide channel to the outside.

[0015] The following description, in a clear and easy-to-understand manner and with reference to the accompanying drawings, will further explain the above-mentioned characteristics, technical features, advantages, and implementation methods of a rodent laboratory animal perfusion cannulation device. Attached Figure Description

[0016] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the scope of this application. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings: Figure 1 This is a schematic diagram illustrating one embodiment of a cannulation device for rodent laboratory animals.

[0017] Figure 2 A schematic diagram illustrating one possible implementation of the adjustment unit.

[0018] Figure 3 A schematic diagram illustrating one possible implementation of the sliding plate and adjusting arm.

[0019] Figure 4 This is a schematic diagram illustrating one embodiment of the sliding plate and the first sliding member.

[0020] Figure 5 This diagram illustrates one possible implementation of the first drive rod.

[0021] Figure 6 This diagram illustrates the connection between the first and second sliders.

[0022] Figure 7 This diagram illustrates the connection between the second drive rod and the second slider.

[0023] Figure 8 A schematic diagram illustrating one embodiment of the rotating part.

[0024] Figure 9 A schematic diagram illustrating the direction of extension of the rotating shaft.

[0025] Figure 10 A schematic diagram illustrating one embodiment of the clamping part.

[0026] Figure 11 A schematic diagram illustrating one embodiment of the irrigation treatment platform.

[0027] Figure 12 This is a schematic diagram illustrating the structure of the liquid collecting device.

[0028] Figure 13 A cross-sectional schematic diagram used to illustrate the liquid collection component.

[0029] Label Explanation 1. Mounting base; 2. Mounting arm; 21. Fixing sleeve; 22. Mounting rod; 23. Mounting part; 3. Adjusting arm; 31. Limiting groove; 32. First drive rod; 321. First threaded section; 322. First force-applying section; 4. Sliding plate; 41. Sliding protrusion; 411. Threaded channel; 42. First slide rail; 43. First threaded groove; 5. First sliding element; 51. First slide groove; 52. First clearance groove; 53. Second slide rail; 54. Second drive rod; 541. Second threaded section; 542. Second force-applying section; 55. First connecting rod 56. Connecting ring; 6. Second threaded groove; 7. Second sliding member; 8. Second sliding groove; 9. Second clearance groove; 10. Third drive rod; 11. Third threaded section; 12. Third force-applying section; 13. Rotating part; 14. Rotating base; 15. Spherical mounting groove; 16. Opening groove; 17. Rotating member; 18. Ball joint; 19. Connecting shaft; 10. Clamping part; 11. Clamping arm; 12. Clamping end; 13. Force-bearing end; 14. Support frame; 15. Drainage grid; 16. Liquid collection part; 17. Guide groove; 18. Drain pipe. Detailed Implementation

[0030] To provide a clearer understanding of the technical features, objectives, and effects of this application, specific embodiments of this application are now described with reference to the accompanying drawings. In the drawings, the same reference numerals indicate components with the same or similar structures but the same function.

[0031] In this document, “illustrative” means “serving as an example, illustration or description”, and any illustration or implementation described herein as “illustrative” should not be construed as a more preferred or advantageous technical solution.

[0032] In experimental animal perfusion experiments, such as the isolated liver perfusion experiment of rats, the experiment first involves making a surgical incision in the abdomen of the experimental rat to expose the liver. Then, using instruments such as cotton swabs, the small intestine of the experimental rat is moved to one side to expose the portal vein and the inferior vena cava. The portal vein is formed by the confluence of the superior mesenteric vein and the splenic vein, and it passes through the hepatoduodenal ligament to enter the porta hepatis. The main portal vein of adult Wistar rats (250-300g) is approximately 15mm-20mm long and 1.6mm-2.5mm in diameter. When performing cannulation (usually using a puncture needle), to ensure a secure ligation and without affecting the blood supply to the liver lobe, the puncture point is typically 5mm-8mm above the confluence. The needle must penetrate deep into the abdominal cavity and have a linear travel of at least 30mm in the insertion direction to cover the entire process from puncture point location to complete cannulation. In current experiments, the above cannulation procedures are usually performed manually, requiring a high level of skill from the experimenter. Furthermore, because the puncture is done manually, vibration is unavoidable during needle insertion, which can cause the needle tip to contact or even scratch the inner wall of the rat's blood vessel, potentially leading to thrombosis or vascular dissection.

[0033] Figure 1 This is a schematic diagram illustrating one embodiment of a cannulation device for rodent laboratory animals. Figure 2 A schematic diagram illustrating one possible implementation of the adjustment unit.

[0034] Combination Figure 1-2 This rodent laboratory animal perfusion cannulation device includes: a mounting base 1, a mounting support arm 2, an adjustment unit, and a cannulation unit, such as... Figure 1 As shown, the mounting base 1 can be a plate-like structure, which is placed or fixed on the experimental platform (desktop) during use. Of course, if it is placed on the laboratory platform, the mounting base 1 needs to have sufficient weight to maintain a stable state during the experiment. Therefore, under this usage requirement, the mounting base 1 can be a plate-like structure made of metal material with a certain thickness, such as stainless steel.

[0035] Combination Figure 1 In the thickness direction of the mounting base 1, the mounting arm 2 is fixedly disposed on the top end face of the mounting base 1, such as... Figure 1-2 As shown, the mounting arm 2 has a mounting part 23 at the end opposite to the mounting base 1.

[0036] Combination Figure 1-2 The adjustment unit includes an adjustment arm 3, a first sliding member 5, and a second sliding member 6, such as... Figure 1 As shown, the adjusting arm 3 is connected to the mounting arm 2 via the mounting part 23, and the length extension direction of the adjusting arm 3 (i.e., Figure 1-2The Y direction in the figure is perpendicular to the extension direction of the mounting arm 2. With this setting, one end of the adjusting arm 3 can be as far away from the mounting base 1 as possible. This can prevent the mounting base 1 and the mounting arm 2 from interfering with the experimental operation during the experiment, thus providing the experimenter with a larger operating space.

[0037] like Figure 1-2 As shown, a sliding plate 4 is provided on one side of the adjusting arm 3. The sliding plate 4 can move relative to the adjusting arm 3 along... Figure 1-2 The sliding plate 4 moves in the Y direction to move closer to or further away from the mounting arm 2, while the first sliding member 5 is located on the side of the sliding plate 4 away from the adjusting arm 3, in combination with... Figure 1 , Figure 1-2 The Z direction is defined as the first direction, which is perpendicular to the length extension direction of the adjusting arm 3. The first sliding member 5 can be translated relative to the sliding plate 4 along the first direction, thereby changing the position of the first sliding member 5 in space. Of course, when the sliding plate 4 moves relative to the adjusting arm 3, the first sliding member 5 will move with the sliding plate 4, thus allowing the first sliding member 5 to have a larger adjustment range.

[0038] Combination Figure 1-2 Correspondingly, the second sliding member 6 is also located on the side of the sliding plate 4 opposite to the adjusting arm 3, and the second sliding member 6 is connected to the first sliding member 5, as shown below. Figure 1-2 As shown, Figure 1-2 The X direction in the figure is the second direction, which is also perpendicular to the extension direction of the adjusting arm 3. Figure 1-2 The second sliding member 6 can translate relative to the first sliding member 5 along the second direction (Y direction), and this second direction is also perpendicular to the first direction. Of course, when the sliding plate 4 moves relative to the adjusting arm 3, or when the first sliding member 5 moves relative to the sliding plate 4, the second sliding member 6 moves with the sliding plate 4 or the first sliding member 5.

[0039] Combination Figure 2 The cannulation unit includes a rotating part 7 and a clamping part 8. The rotating part 7 is located on the side of the second sliding member 6 opposite to the first sliding member 5, and the rotating part 7 has a rotating shaft (see reference). Figure 9 , Figure 9 In section A), the rotating shaft is parallel to the second direction and perpendicular to the extension direction of the adjusting arm 3; the clamping part 8 is connected to the rotating part 7 and is used to externally connect the puncture needle. When the rotating part 7 rotates around the rotating axis, the clamping part 8 swings accordingly, thereby adjusting the insertion pitch angle of the puncture needle.

[0040] Based on the above, in practical use, the anesthetized experimental rat is fixed supine on the operating table, positioned below the clamping part 8, with the rat's head and tail facing parallel to the second direction. Figure 1-2(In the X-direction), expose the rat liver and porta hepatis using standard surgical procedures, separate the portal vein, and use a cotton swab to move the small intestine to one side to clearly expose the main trunk of the portal vein and its confluence. Clamp the puncture needle to clamp part 8, and then perform pre-puncture positioning using the adjustment unit. The specific adjustment steps are as follows (the adjustment steps described below are not in any particular order): First, adjust the sliding plate 4 to move along the Y direction (the direction of the extension of the length of the adjusting arm 3) in the figure so that the tip of the puncture needle approaches the portal vein area from above the incision in the rat's abdomen; The second step is to adjust the first sliding member 5 to move along the first direction, thereby changing the depth position of the needle tip relative to the tissue. Third step, adjust the second slider 6 to move along the second direction so that the needle tip is aligned with the predetermined puncture point about 5mm-8mm above the confluence of the portal vein in the horizontal plane; Then, rotate the rotating part 7 to adjust the tilt angle of the puncture needle so that it is consistent with the direction of the portal vein, ensuring that the needle insertion path is parallel to the long axis of the blood vessel.

[0041] After completing the above position and attitude adjustments, combined with Figure 1-2 The second sliding member 6 is driven to move along the second direction. At this time, the clamping part 8 moves synchronously with the second sliding member 6 until it is inserted into the predetermined puncture point. Since the second sliding member 6 has a long stroke along the second direction on the first sliding member 5, the entire needle insertion process can provide a linear stroke of not less than 30 mm, which is sufficient to cover the entire process from puncture point positioning to the complete insertion of the puncture needle into the portal vein trunk.

[0042] As will be understood by those skilled in the art, when the head and tail of the rat are parallel to the length extension direction of the adjusting arm 3, the needle insertion operation is achieved by driving the sliding plate 4 to move relative to the adjusting arm 3.

[0043] In the above configuration, the mounting base 1, the mounting arm 2, and the adjusting arm 3 constitute a rigid support device, which can provide sufficient and stable support when the sliding plate 4, the first sliding member 5, and the second sliding member 6 move. At the same time, through the sliding cooperation between the sliding plate 4, the first sliding member 5, and the second sliding member 6, the relative positional relationship between the clamping part 8 and the experimental rat can be adjusted in multiple dimensions. This not only adapts to rats of different sizes, but also, through the relatively stable sliding between the sliding plate 4, the first sliding member 5, and the second sliding member 6, the puncture needle is smoothly inserted into the target blood vessel, eliminating the shaking that is unavoidable during manual operation, preventing the needle tip from contacting or scratching the inner wall of the blood vessel, and effectively avoiding the risk of complications such as thrombosis and vascular dissection.

[0044] Furthermore, under the above configuration, the sliding plate 4, the first sliding member 5, and the second sliding member 6 provide translation on the X (second direction), Y (length extension direction of the adjusting arm 3), and Z (first direction) axes, respectively. At the same time, combined with the axial rotation of the rotating part 7, the puncture needle tip can be accurately aligned with the target puncture point in space, and the needle tip angle can be consistent with the course of the blood vessel, so that the puncture position and depth remain consistent. This is beneficial for the repetition of experimental operations and reduces the requirements for the operator's skills.

[0045] Furthermore, by moving the sliding plate 4, the first sliding member 5, and the second sliding member 6 relative to each other, the path of the puncture needle can be corrected during the needle insertion process, thereby minimizing damage to blood vessels.

[0046] like Figure 3 As shown, the mounting arm 2 includes a fixing sleeve 21 and a mounting rod 22, combined with... Figure 1-3 The mounting rod 22 is inserted into the fixed sleeve 21 and can move axially relative to the fixed sleeve 21. In this configuration, for example, when the mounting rod 22 moves away from the mounting base 1 relative to the fixed sleeve 21, the distance between the adjusting arm 3 and the mounting base 1 can be increased, thereby further adjusting the position between the clamping part 8 and the rat to be tested.

[0047] Of course, combined Figure 2-3 After the mounting rod 22 moves relative to the fixed sleeve 21, it is necessary to fix the position of the mounting rod 22 after the movement. Therefore, in combination with Figure 2 A first threaded hole is provided on the outer surface of the fixed sleeve 21, and a bolt is screwed into the first threaded hole so that the bolt abuts against the outer wall of the mounting rod 22, thereby fixing the mounting rod 22 inside the fixed sleeve 21, so that the mounting rod 22 will not move during the experiment.

[0048] The mounting part 23 is located at the end of the mounting rod 22 that is away from the mounting base 1, such as Figure 1-2 As shown, the mounting part 23 has a mounting channel, and the adjusting arm 3 is installed into the mounting channel. Specifically, a second threaded hole is provided on the outer wall of the mounting part 23, and a bolt is screwed into the second threaded hole to fix the adjusting arm 3 into the mounting channel. In this arrangement, not only can a stable connection be formed between the adjusting arm 3 and the mounting rod 22, but the bolt can also be loosened according to the usage requirements or the requirements for operating space to adjust the relative position between the adjusting arm 3 and the mounting rod 22. This allows the sliding plate 4, the first sliding member 5, and the second sliding member 6 to be closer to or further away from the mounting rod 22, thereby obtaining a suitable or larger operating space.

[0049] Specifically, such as Figure 2-3 As shown, the adjusting arm 3 is roughly rectangular. Correspondingly, the shape of the mounting hole is the same as the shape of the outer contour of the adjusting arm 3. With this setting, the rotation of the adjusting arm 3 relative to the mounting rod 22 can be effectively avoided. Thus, during the experiment, the clamping part 8 can be guaranteed not to rotate undesirably.

[0050] Figure 3 This is a schematic structural diagram illustrating one possible embodiment of the sliding plate and adjusting arm. To achieve movement of the sliding plate 4 along the length extension direction of the adjusting arm 3, as follows... Figure 3 As shown, in this embodiment, a first drive rod 32 is also included, wherein the first drive rod 32 includes a first threaded section 321 and a first force-applying section 322, as shown. Figure 3 As shown, a limiting groove 31 is provided on one side of the adjusting arm 3, and a sliding protrusion 41 is provided on the side of the sliding plate 4 facing the adjusting arm 3. The sliding protrusion 41 is slidably installed in the limiting groove 31. The sliding protrusion 41 has a threaded channel 411. The first threaded section 321 of the first drive rod 32 passes through the limiting groove 31 from the end of the adjusting arm 3 away from the mounting rod 22 and is screwed into the threaded channel 411 of the sliding protrusion 41. The first drive rod 32 can rotate axially relative to the adjusting arm 3. When the first drive rod 32 rotates axially, the inner wall of the limiting groove 31 restricts the sliding protrusion 41, so that the sliding plate 4 cannot rotate with the first drive rod 32. It only slides along the length extension direction of the adjusting arm 3 with the cooperation of the first threaded section 321 and the threaded channel 411. Under the constraint of the limiting groove 31, the rotational motion of the first drive rod 32 can be converted into linear motion through the cooperation of the first threaded section 321 and the threaded channel 411. This allows the sliding plate 4 to move smoothly along the length extension direction of the adjusting arm 3. At the same time, this threaded cooperation method has a self-locking characteristic, which allows the sliding plate 4 to stop stably at any position on the adjusting arm 3. Therefore, when inserting a needle in the length extension direction of the adjusting arm 3, not only can vibration be avoided, but the needle insertion depth can also be precisely adjusted. Correspondingly, when inserting a needle in the second direction, this setting method can precisely adjust the position of the sliding plate 4 on the adjusting arm 3 according to the usage requirements. In addition, the sliding plate 4 will not slide unexpectedly due to gravity or external force during the experimental operation.

[0051] Figure 4 This is a schematic diagram illustrating one embodiment of the sliding plate and the first sliding member. Figure 5 This diagram illustrates one possible implementation of the first drive rod. (Combined with...) Figure 4-5The sliding plate 4 has two first slide rails 42 on the side opposite to the adjusting arm 3. Correspondingly, the first sliding member 5 has two first slide grooves on the corresponding side, and the first sliding member 5 is connected to the sliding plate 4 through the first slide grooves and the first slide rails 42. Of course, the positions of the first slide rails 42 and the first slide grooves can be interchanged. For example, the first slide rails 42 can be located on the first sliding member 5, and the first slide grooves can be located on the sliding plate 4.

[0052] Meanwhile, to drive the first sliding member 5 to move in the first direction, a second driving rod 54 is provided between the first sliding member 5 and the sliding plate 4. The second driving rod 54 includes a first threaded section 321 and a second force-applying section 542 connected in sequence, such as... Figure 4-5 As shown, a first threaded groove 43 is provided between the two first slide rails 42, and a first clearance groove 52 is provided on the corresponding side of the first sliding member 5. The first threaded groove 43 and the first clearance groove 52 are both semi-circular in cross-section perpendicular to the first direction. After the first sliding member 5 is connected to the sliding plate 4 through the cooperation of the second slide groove 61 and the first slide rail 42, the first threaded groove 43 and the first clearance groove 52 are combined to form a circular channel. The second drive rod 54 is rotatably connected to the first sliding member 5 through the second force application section 542. The second threaded section 541 extends into the first threaded groove 43 and the first clearance groove 52, and the second threaded section 541 is engaged with the thread on the inner wall of the first threaded groove 43.

[0053] In the above configuration, although the first sliding member 5 is connected to the sliding plate 4 through the first sliding groove and the first sliding rail 42, the self-locking characteristic brought about by the threaded engagement of the second threaded section 541 and the first threaded groove 43 can prevent the first sliding member 5 from sliding relative to the sliding plate 4 due to gravity or slight external force. Thus, no additional structural design is required to maintain the connection stability between the first sliding member 5 and the sliding plate 4.

[0054] Meanwhile, since the second drive rod 54 is rotatably connected to the first sliding member 5, when the second drive rod 54 rotates, the rotational motion of the second drive rod 54 can be converted into linear motion by utilizing the sliding engagement between the second slide groove 61 and the first slide rail 42 and the threaded engagement between the second threaded section 541 and the first threaded groove 43, thereby precisely adjusting the relative position between the first sliding member 5 and the sliding plate 4.

[0055] In addition, the second drive rod 54 is located between the first sliding member 5 and the sliding plate 4, so that most of the length of the second drive rod 54 is hidden. This not only avoids taking up extra external space of the device, but also makes the overall structure of the device more compact and lighter, thereby reducing the weight requirements of the mounting base 1.

[0056] Specifically, in the first direction, the top end face of the first sliding member 5 is provided with a first connecting ring 55, which is concentric with the clearance groove. The second threaded section 541 of the second drive rod 54 passes through the first connecting ring 55, and the second force-applying section 542 is rotatably connected to the first connecting ring 55, so that the second drive rod 54 can only rotate axially relative to the first sliding member 5, but cannot move axially.

[0057] Figure 6 This diagram illustrates the connection between the first and second sliders. Figure 7 This diagram illustrates the connection between the second drive rod and the second sliding member. To achieve a sliding connection between the second sliding member 6 and the first sliding member 5, as shown... Figure 6-7 As shown, in this embodiment, a second slide rail 53 is provided on one side of the first sliding member 5, and a second slide groove 61 is provided on the side of the second sliding member 6 facing the first sliding member 5. The first sliding member 5 and the second sliding member 6 are slidably connected by the cooperation of the second slide rail 53 and the second slide groove 61. Of course, the positions of the second slide rail 53 and the second slide groove 61 can be interchanged. For example, the second slide rail 53 can be provided on the second sliding member 6, and the second slide groove 61 can be provided on the first sliding member 5.

[0058] Combination Figure 6-7 To drive the second sliding member 6 to move relative to the first sliding member 5 along the second direction, a third driving rod 63 is provided between the first sliding member 5 and the second sliding member 6. The third driving rod 63 includes a third threaded section 631 and a third force-applying section, such as... Figure 6-7 As shown, a second threaded groove 56 is formed on the side of the first sliding member 5 facing the second sliding member 6, and a second clearance groove 62 is formed on the side of the second sliding member 6 facing the first sliding member 5. Similarly, the cross sections of the second threaded groove 56 and the second clearance groove 62 perpendicular to the second direction are both semi-circular. After the first sliding member 5 and the second sliding member 6 are connected, the second threaded groove 56 and the second clearance groove 62 combine to form a circular channel. The third drive rod 63 is rotatably connected to the second sliding member 6 through the third force application section. The third threaded section 631 extends into the second threaded groove 56 and the second clearance groove 62, and the third threaded section 631 engages with the thread on the inner wall of the second threaded groove 56.

[0059] With the above settings, the self-locking characteristic brought about by the threaded engagement between the third threaded section 631 and the second threaded groove 56 ensures that the second sliding member 6 will not slip relative to the first sliding member 5 due to external force. Thus, no additional structural design is required to maintain the connection stability between the first sliding member 5 and the sliding plate 4. At the same time, the relative position between the second sliding member and the first sliding member 5 can be precisely adjusted.

[0060] In addition, the second drive rod 54 is located between the first sliding member 5 and the sliding plate 4, and the third drive rod 63 is located between the second sliding member 6 and the first sliding member 5. This allows most of the length of the second drive rod 54 and the third drive rod 63 to be concealed inside the structure, without occupying additional external space of the device. This makes the overall structure of the device more compact and lighter, and minimizes the requirements for the counterweight of the mounting base 1.

[0061] Figure 8 A schematic diagram illustrating one embodiment of the rotating part. Figure 9 This is a schematic diagram illustrating the extension direction of the rotating shaft. The rotating part 7 includes a rotating base 71 and a rotating member 73. The rotating base 71 is fixedly disposed on the side of the second sliding member 6 opposite to the first sliding member 5, as shown below. Figure 8-9 As shown, the rotating base 71 has a spherical mounting body, the interior of which is formed with a spherical mounting groove 72, and the outer surface of the spherical mounting body has an opening groove 721 communicating with the spherical mounting groove 72; the rotating component 73 includes a ball joint 731 and a connecting shaft 732 connected in sequence, combined with Figure 8-9 The ball joint 731 is disposed within the spherical mounting groove 72, and the connecting shaft 732 extends out from the opening groove 721. Specifically, for example... Figure 9 As shown, the opening groove 721 surrounds Figure 9 The rotating shaft A is circumferentially located on the outer surface of the spherical mount, and the connecting shaft 732 and the two sides of the opening groove 721 are tangent to each other. In this configuration, the ball joint 731 and the spherical mounting groove 72 cooperate to form a tangential constraint on the connecting shaft 732 through the two side walls of the opening groove 721, restricting the ball joint 731 to a single degree of freedom of swinging only around the rotating shaft. In this way, when adjusting the angle of the puncture needle, the needle tip will only change the pitch angle in the sagittal plane of the blood vessel, without any lateral sway, ensuring that the needle insertion direction is always parallel to the long axis of the blood vessel.

[0062] When the needle is inserted in the second direction, the resistance received by the puncture needle is transmitted to the connecting shaft 732 through the clamping part 8. At this time, the two sides of the opening groove 721 support the connecting shaft 732 to resist the resistance generated during the puncture process, so that the connecting shaft 732 will not wobble.

[0063] Of course, in actual use, it is required that the ball joint 731 cannot rotate. Therefore, in combination with Figure 8-9 As can be seen, a third threaded hole is also provided on the outer surface of the spherical mounting body, and a bolt is screwed into the third threaded hole so that the end of the bolt abuts against the outer surface of the ball joint 731, thereby restricting the rotation of the ball joint 731.

[0064] In addition, to facilitate the operator's observation of the rotation angle of the ball joint 731, a corresponding angle mark is provided on the outer surface of the ball joint 731. This angle mark is exposed in the opening groove 721, so as to facilitate the operator's judgment of the rotation amount of the ball joint 731.

[0065] Figure 10 This is a schematic structural diagram illustrating an embodiment of the clamping part. The clamping part 8 includes: a pair of clamping arms 81 and an elastic member. The pair of clamping arms 81 are rotatably connected, and each clamping arm 81 has a clamping end 811 and a force-receiving end 812. The force-receiving end 812 of one clamping arm 81 is detachably connected to a connecting part. The elastic member is disposed between the pair of clamping arms 81 and abuts against the pair of clamping arms 81 respectively. The elastic member can provide elastic force to the pair of clamping arms 81 so that the clamping ends 811 of the pair of clamping arms 81 always abut against each other.

[0066] Specifically, each clamping arm 81 has a through hole at its force-bearing end 812, and correspondingly, the connecting shaft 732 has a fourth threaded hole at the end opposite to the ball joint 731. A bolt is then passed through the through hole and screwed into the fourth threaded hole to connect the clamping part 8 to the rotating part 7.

[0067] Under the action of the elastic element, the clamping ends 811 of a pair of clamping arms 81 are always in contact, while the two force-bearing ends 812 are far apart. In actual use, the needle tip of the puncture needle passes through the pair of clamping arms 81 and extends out between the two clamping ends 811, thereby realizing the puncture assembly to the clamping part 8.

[0068] Figure 11 A schematic diagram illustrating one embodiment of the irrigation treatment platform. Figure 12 This is a schematic diagram illustrating the structure of the liquid collecting device. Figure 13 This is a cross-sectional schematic diagram illustrating the liquid collection component. The equipment also includes: an irrigation treatment platform for placing experimental rats and collecting waste liquid generated during experimental procedures, such as... Figure 11 As shown, the irrigation treatment platform includes a support frame 9 and a drainage grid 91. The support frame 9 has an installation groove, and the drainage grid 91 is installed within the installation groove. Figure 11 As shown, the drainage grid 91 has a mesh structure. In actual use, the irrigation treatment platform can be placed on a liquid container of the corresponding shape, and then the experimental rat can be placed on the drainage grid 91 for irrigation. During the operation, the waste liquid generated by irrigation flows into the liquid container below through the mesh of the drainage grid 91, thereby preventing the waste liquid from spreading to the experimental platform.

[0069] To further collect waste liquid, the waste treatment platform also includes: a waste liquid discharge unit, such as... Figure 11-13As shown, the waste liquid discharge unit includes a liquid collection component 92 and a liquid discharge pipe 94. The liquid collection component 92 is located below the support frame 9 in the thickness direction. The liquid collection component 92 has a guide channel 921, and the liquid discharge pipe is connected to the guide channel 921. The cross-section of the guide channel 921 is funnel-shaped. In actual use, the liquid discharge pipe 94 is usually connected to the waste liquid pool in the laboratory. With this arrangement, the waste liquid generated during the experiment first flows into the guide channel 921. The funnel-shaped guide channel 921 can avoid liquid residue, thereby guiding all the waste liquid generated during the experiment to the waste liquid pool.

[0070] To keep the drawings concise, only the parts relevant to this application are shown schematically in each drawing, and they do not represent the actual structure of the product. In addition, to make the drawings concise and easy to understand, in some drawings, only one of the components with the same structure or function is shown schematically, or only one of them is labeled.

[0071] It should be understood that although this specification describes various embodiments, not every embodiment contains only one independent technical solution. This way of describing the specification is only for clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

[0072] The detailed descriptions listed above are merely specific descriptions of feasible embodiments of this application and are not intended to limit the scope of protection of this application. All equivalent implementations or modifications made without departing from the spirit of the art of this application, such as combinations, divisions or repetitions of features, should be included within the scope of protection of this application.

Claims

1. A perfusion cannulation device for rodent laboratory animals, characterized in that, It includes, Mounting base; The mounting arm is located on the top end face of the mounting base and has a mounting portion facing away from the mounting base; The adjustment unit includes: An adjusting arm is connected to the mounting portion. A sliding plate is provided on one side of the adjusting arm, and the sliding plate is movable along the extending direction of the adjusting arm to move closer to or further away from the mounting arm. A first sliding member is disposed on the side of the sliding plate opposite to the adjusting arm, and is capable of sliding relative to the sliding plate along a first direction; and The second slider is located on the side of the first slider opposite to the sliding plate, and is capable of sliding relative to the first slider in a second direction. Wherein, both the first direction and the second direction are perpendicular to the extension direction of the adjusting arm, and the second direction is perpendicular to the first direction; The intubation unit includes a rotating part and a clamping part. The rotating part is rotatably connected to the second sliding member on the side opposite to the first sliding member. The rotating part has a rotating shaft parallel to the second direction. The clamping part is connected to the rotating part and is used to clamp the irrigation device.

2. The rodent laboratory animal perfusion cannulation device as described in claim 1, characterized in that, The adjustment unit also includes a first drive rod; A limiting groove is provided on one side of the adjusting arm, and a sliding protrusion is provided on the side of the sliding plate facing the adjusting arm. The sliding protrusion is slidably engaged in the limiting groove, and a threaded hole is provided on the sliding protrusion. The first drive rod is rotatably mounted on the adjusting arm, and the first drive rod passes through the limiting slide groove from the end of the adjusting arm away from the mounting arm, and is threadedly engaged with the threaded hole, so that when the first drive rod is rotated, the sliding plate is driven to move along the extension direction of the adjusting arm.

3. The rodent laboratory animal perfusion cannulation device as described in claim 2, characterized in that, One of the sliding plate and the first sliding member is provided with a first slide rail, and the other is provided with a first slide groove. The first sliding member is connected to the sliding plate through the first slide groove and the first slide rail. The sliding plate has a first threaded groove on the side away from the adjusting arm, and the first sliding member has a first clearance groove on the side facing the sliding plate. The adjustment unit further includes: a second drive rod, which is disposed between the first threaded groove and the first clearance groove, and the second drive rod is rotatably connected to the first sliding member; The second drive rod is also threadedly engaged with the first threaded groove, and when the second drive rod rotates relative to the first sliding member, it can drive the first sliding member to move in the first direction.

4. The rodent laboratory animal perfusion cannulation device as described in claim 3, characterized in that, The first sliding member has a first connecting ring at one end in the first direction. The first connecting ring is concentric with the first clearance groove. The second driving rod passes through the first connecting ring and is rotatably connected to the first connecting ring to restrict the axial movement of the second driving rod relative to the first sliding member.

5. The rodent laboratory animal perfusion cannulation device as described in claim 2, characterized in that, One of the first sliding member and the second sliding member is provided with a second slide rail, and the other is provided with a second slide groove. The second sliding member is connected to the first sliding member through the second slide groove and the second slide rail. The first sliding member has a second threaded groove on the side facing the second sliding member, and the second sliding member has a second clearance groove on the side facing the first sliding member. The adjustment unit further includes: a third drive rod, which is disposed between the second threaded groove and the second threaded groove, and is rotatably connected to the second sliding member; The third drive rod is also threadedly engaged with the second threaded groove, and when the third drive rod rotates relative to the second sliding member, it can drive the second sliding member to move in the second direction.

6. The rodent laboratory animal perfusion cannulation device as described in claim 1, characterized in that, The rotating part includes: A rotating base is fixed to the side of the second sliding member away from the first sliding member. The rotating base has a spherical mounting body. A spherical mounting groove is formed inside the spherical mounting body, and an opening groove communicating with the spherical mounting groove is opened on its outer surface. The opening groove extends circumferentially around the rotating axis. A rotating component includes a ball joint and a connecting shaft. The ball joint is housed in the spherical mounting groove, and the connecting shaft extends from the opening groove and is tangent to the two side walls of the opening groove, thereby restricting the rotating component to swing only around the rotating shaft. The clamping part is connected to the end of the connecting shaft opposite to the ball joint.

7. The rodent laboratory animal perfusion cannulation device as described in claim 6, characterized in that, The rotating part also includes a bolt, wherein the outer surface of the spherical mounting body is provided with a threaded hole, the bolt is screwed into the spherical mounting groove through the threaded hole, and the end of the bolt abuts against the outer surface of the ball joint to lock the rotating part.

8. The rodent laboratory animal perfusion cannulation device as described in claim 6, characterized in that, The clamping part includes: a pair of clamping arms and an elastic member, the pair of clamping arms being rotatably connected, each clamping arm having a clamping end and a force-receiving end, the force-receiving end of one of the clamping arms being detachably connected to the connecting shaft, the elastic member being disposed between the pair of clamping arms and abutting against the pair of clamping arms respectively, providing elastic force to the clamping arms so that the clamping ends of the pair of clamping arms are always abutting against each other.

9. The rodent laboratory animal perfusion cannulation device as described in claim 1, characterized in that, The rodent laboratory animal perfusion cannulation device also includes: An irrigation treatment platform includes a support frame and a drainage grid, wherein the support frame has an installation groove and the drainage grid is disposed in the installation groove.

10. The rodent laboratory animal perfusion cannulation device as described in claim 9, characterized in that, The irrigation treatment platform also includes: A waste liquid discharge unit includes a liquid collection component and a liquid discharge pipe. In the thickness direction of the support frame, the liquid collection component is located below the support frame. The liquid collection component has a guide channel, and the liquid discharge pipe is connected to the guide channel to discharge the liquid in the guide channel to the outside.

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