A soothing anti-inflammatory antioxidant composition and methods of making and using same

Abstract

The present application provides a composition comprising one of vitamin B3 or caffeine and thiouric acid; the mass ratio of one of vitamin B3 or caffeine and thiouric acid is 1:2-2:1. The present inventors select the specific components and the specific ratio to make the composition have synergistic effect in antioxidation, anti-inflammation and soothing, and good efficacy.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a soothing, anti-inflammatory, and antioxidant composition, its preparation method, and its application. Background Technology

[0002] The skin is the body's first line of defense, playing multiple roles in maintaining life. For example, it prevents water evaporation and electrolyte loss; maintains body temperature; and blocks pathogens and toxins from entering the body. When exposed to exogenous factors such as ultraviolet radiation, dust particles, and medications, or endogenous factors such as ischemia and hormone metabolism, the skin may become infected. To cope with infection, the body produces oxidative stress, releasing large amounts of reactive oxygen species (ROS), which promote inflammation. Inflammation is a natural defense response of the body against external stimuli and pathogenic microorganisms. Excessive inflammation can disrupt the body's immune system, leading to damage to tissue structures. On the skin, this manifests as seborrheic dermatitis, contact dermatitis, allergic dermatitis, steroid-dependent dermatitis, atopic dermatitis, and neurodermatitis. Currently, the typical medication for these skin inflammations is corticosteroids.

[0003] Glucocorticoids can be used to treat inflammation, but long-term use can lead to side effects such as exogenous Cushing's syndrome. Therefore, finding a new anti-inflammatory combination is of great significance. Summary of the Invention

[0004] In view of this, the technical problem to be solved by the present invention is to provide a composition that has synergistic anti-inflammatory and antioxidant effects.

[0005] This invention provides a composition comprising one of vitamin B3 or caffeine and thiouric acid;

[0006] The mass ratio of one of the vitamin B3 or caffeine to thiouric acid is 1:2 to 2:1.

[0007] The composition provided by this invention comprises thiouric acid, CAS number 6736-42-1, with the molecular formula C6H6N2O2S; its structural formula is as follows:

[0008]

[0009] The thiouric acid of this invention is applicable to the cosmetics field. Thiouric acid can scavenge ABTS and DPPH free radicals, exhibiting antioxidant effects that not only aid in its soothing effects but also brighten skin tone. Thiouric acid can reduce the levels of inflammatory factors IL-6 and TNF-α, providing a soothing effect and addressing facial inflammation issues in sensitive individuals.

[0010] This invention is the first to use thiouric acid in the cosmetics field. At the same time, this invention creatively discovers that one of vitamin B3 or caffeine has a synergistic effect with thiouric acid, synergistically improving the soothing, anti-inflammatory and antioxidant effects.

[0011] In this invention, the mass ratio of one of the vitamin B3 or caffeine to thiouric acid is 1:2 to 2:1.

[0012] In some specific embodiments, the mass ratio of one of vitamin B3 or caffeine to thiouric acid is 1:1, 1:2 or 2:1; or any ratio range between the two.

[0013] In a preferred embodiment of the present invention, the mass ratio of one of the vitamin B3 or caffeine to thiouric acid is 1:1.

[0014] In some specific implementation plans, the amount of vitamin B3 or caffeine added is 100 μg / mL; at the same time, the amount of thiouric acid added is 100 μg / mL;

[0015] In some specific implementation schemes, the amount of vitamin B3 or caffeine added is 1000 μg / mL; at the same time, the amount of thiouric acid added is 1000 μg / mL.

[0016] This invention provides the application of the above-mentioned soothing, anti-inflammatory, and antioxidant composition in the preparation of soothing, anti-inflammatory, and antioxidant products.

[0017] The inventors have discovered that thiouric acid and vitamin B3 have synergistic antioxidant effects; thiouric acid and caffeine also have synergistic antioxidant effects.

[0018] Thiouronic acid and vitamin B3 have a synergistic anti-inflammatory effect; thiouronic acid and caffeine have a synergistic anti-inflammatory effect.

[0019] Thiouronic acid and vitamin B3 have a soothing effect; thiouronic acid and caffeine have a soothing effect.

[0020] According to the present invention, the antioxidants include scavenging ABTS free radicals and DPPH free radicals;

[0021] The anti-inflammatory effects include reducing the levels of inflammatory factors IL-6 and TNF-α;

[0022] The soothing effects include relieving pain, reducing redness, and / or brightening.

[0023] Throughout this manual, the term "oxidation" refers to "reactive oxygen species (ROS)," a chemically reactive molecule containing oxygen atoms that attacks biological tissues and damages cells as oxygen compounds produced within the body. It is a type of oxygen with strong oxidizing capabilities, also known as reactive oxygen species. ROS molecules contain oxygen ions and hydrogen peroxide, and are highly reactive due to their unpaired electrons. ROS are naturally produced by the normal metabolism of oxygen and are known to play important roles in cell signaling and homeostasis. However, due to various environmental stresses, such as exposure to ultraviolet radiation, radiation, or high temperatures, there is a risk of a rapid increase in ROS concentration, which can damage cell structure. Through this phenomenon, known as oxidative stress, excessively increased ROS can react indiscriminately, transforming into substances harmful to the organism; specifically, it can accelerate aging, cause cancer, and induce inflammation.

[0024] This invention provides a soothing, anti-inflammatory, and antioxidant product, the raw materials of which include the soothing, anti-inflammatory, and antioxidant composition described in the above technical solution.

[0025] In one preferred embodiment of the present invention, the product is a cosmetic. The present invention also includes excipients commonly used in the cosmetic field, and is not limited thereto.

[0026] The cosmetics are available in the form of liquid, lotion, emulsion, powder, or cream.

[0027] The cosmetics mentioned can be divided into three categories: cleansing cosmetics, skincare cosmetics, and beauty / decorative cosmetics. Cleansing cosmetics are applied to the surface of the human body (such as the epidermis, hair, nails, lips, etc.) by smearing, spraying, or other similar methods to cleanse and hygienically eliminate unpleasant odors. Skincare cosmetics are applied to the surface of the human body (such as the epidermis, hair, nails, lips, etc.) by smearing, spraying, or other similar methods to maintain skin health. Beauty / decorative cosmetics are applied to the surface of the human body (such as the epidermis, hair, nails, lips, etc.) by smearing, spraying, or other similar methods to beautify, enhance, and increase personal attractiveness.

[0028] Cleansing cosmetics suitable for the skin include: facial cleanser, makeup remover (lotion), cleansing cream (moisturizer), face mask, floral water, antipruritic powder, talcum powder, or bath gel; skin care cosmetics suitable for the skin include skin cream, lotion, or toner; beauty / makeup cosmetics suitable for the skin include: pressed powder, blush, eyeshadow, eyeliner (liquid), eyebrow pencil, perfume, or cologne; cleansing cosmetics suitable for the lips include lip makeup remover; skin care cosmetics suitable for the lips include lip balm; beauty / makeup cosmetics suitable for the lips include: lipstick, lip gloss, or lip liner.

[0029] The present invention provides a soothing, anti-inflammatory, and antioxidant lotion, comprising the following raw materials in parts by weight:

[0030]

[0031]

[0032] When the total quantity is 100 parts, the above weight parts are equivalent to mass percentages.

[0033] In some specific embodiments, the preservative includes p-hydroxyacetophenone and 1,2-hexanediol; the mass ratio of p-hydroxyacetophenone to 1,2-hexanediol is 0.1:0.2;

[0034] In some specific embodiments, the humectant includes glycerin, trehalose, and jojoba seed oil; the mass ratio of glycerin, trehalose, and jojoba seed oil is 3:0.5:0.1.

[0035] In some specific embodiments, the stabilizer / antifreeze agent is dipropylene glycol;

[0036] In some specific embodiments, the antioxidant is vitamin E;

[0037] In some specific embodiments, the emulsifier is cetearyl glucoside.

[0038] In one specific embodiment of the present invention, the raw materials include the following parts by weight:

[0039]

[0040] The present invention also provides a method for preparing the soothing, anti-inflammatory, and antioxidant emulsion according to any one of the above technical solutions, comprising the following steps:

[0041] S1) The preservative is dissolved to obtain phase A;

[0042] One of vitamin B3 or caffeine, thiouric acid, water, humectant, and fixative are pre-dissolved by heating to obtain phase B;

[0043] After the stabilizer / antifreeze and antioxidant swell upon heating, phase C is obtained;

[0044] S2) Add phase C to phase B and homogenize, then add emulsifier and homogenize again. After cooling, add phase A, cool and stir to obtain the final product.

[0045] The components and proportions described above have been clearly described in this invention and will not be repeated here.

[0046] The method for preparing the soothing, anti-inflammatory, and antioxidant emulsion provided by this invention first involves dissolving a preservative to obtain phase A, which is a mixture of hydroxyacetophenone and 1,2-hexanediol; the mass ratio of hydroxyacetophenone to 1,2-hexanediol is 0.1:0.2.

[0047] Phase B is obtained by pre-dissolving one of vitamin B3 or caffeine, thiouric acid, water, a humectant, and a setting agent under heat. The preferred temperature for the heating and dissolution is 55–65°C; more preferably 60°C.

[0048] After the stabilizer / antifreeze and antioxidant are heated and swollen, phase C is obtained. The heating and swelling temperature is preferably 55-65°C; more preferably 60°C.

[0049] Phase C is added to Phase B and homogenized for 2 to 4 minutes, preferably 3 minutes; then an emulsifier is added and homogenized for 2 to 4 minutes, preferably 3 minutes.

[0050] After homogenization, stir and cool to 55-65°C; more preferably 60°C; then add the completely dissolved A phase and continue to cool to room temperature, stirring until homogeneous to obtain the final product.

[0051] The method of using the above-mentioned lotion of the present invention is as follows: after cleansing the face in the morning and evening, apply the soothing, anti-glycation and brightening lotion of the present invention to the entire face; the number of times of use can be selected as 2 times / day.

[0052] This invention provides a composition comprising one of vitamin B3 or caffeine and thiouric acid; wherein the mass ratio of the vitamin B3 or caffeine to thiouric acid is 1:2 to 2:1. By selecting specific components and combining them in a specific ratio, the inventors have achieved a synergistic effect in terms of antioxidation, anti-inflammation, and soothing, resulting in excellent efficacy. Attached Figure Description

[0053] Figure 1 The ability of each experimental group to inhibit TNF-α;

[0054] Figure 2 The ability of each experimental group to inhibit IL-6;

[0055] Figure 3 The ability of each experimental group to scavenge DPPH free radicals;

[0056] Figure 4 The ability of each experimental group to scavenge ABTS free radicals. Detailed Implementation

[0057] This invention provides a soothing, anti-inflammatory, and antioxidant composition, its preparation method, and its application. Those skilled in the art can refer to the content of this document and appropriately modify the process parameters to achieve the desired result. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and fall within the scope of protection of this invention. The method and application of this invention have been described through preferred embodiments. Those skilled in the art can clearly modify or appropriately change and combine the method and application described herein without departing from the content, spirit, and scope of this invention to realize and apply the technology of this invention.

[0058] It should be understood that the expression “one or more of…” individually includes each of the objects described after the expression, as well as various different combinations of two or more of the described objects, unless otherwise understood from the context and usage. The expression “and / or” combined with three or more described objects should be understood to have the same meaning, unless otherwise understood from the context.

[0059] The terms “including,” “having,” or “containing,” including the use of their grammatical synonyms, should generally be understood as open-ended and non-restrictive, for example, not excluding other unstated elements or steps, unless otherwise specifically stated or understood from the context.

[0060] In this application, the term "and / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone. A and B can be singular or plural.

[0061] It should be understood that the order of the steps or the order in which certain actions are performed is not important as long as the invention remains operational. Furthermore, two or more steps or actions can be performed simultaneously.

[0062] The use of any and all instances or exemplary language such as “e.g.” or “including” in this document is merely intended to better illustrate the invention and is not intended to limit the scope of the invention unless the claims are made. No language in this specification should be construed as indicating that any unclaimed element is essential to the practice of the invention.

[0063] Furthermore, the numerical ranges and parameters used to define the present invention are approximate values, and the relevant values ​​in the specific embodiments have been presented as precisely as possible. However, any value inevitably contains standard deviations due to individual test methods. Therefore, unless explicitly stated otherwise, it should be understood that all ranges, quantities, values, and percentages used in this disclosure are modified with the word "approximately." Here, "approximately" generally means an actual value within plus or minus 10%, 5%, 1%, or 0.5% of a particular value or range.

[0064] It should be understood that in the various embodiments of this application, the order of the above processes does not imply the order of execution. Some or all steps may be executed in parallel or sequentially. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of this application.

[0065] The embodiments and comparative examples of this invention describe some examples, in which the embodiments illustrate certain implementations of the invention. However, this does not mean that the effects of the invention can only be achieved in these examples.

[0066] To further illustrate the present invention, the following detailed description, in conjunction with embodiments, provides a soothing, anti-inflammatory, and antioxidant composition, its preparation method, and its application.

[0067] All reagents used in the following examples are commercially available; among them, mouse macrophages (RAW264.7) were obtained from Wuhan Pronosei Biotechnology Co., Ltd.; RAW264.7-specific culture medium was obtained from Wuhan Pronosei Biotechnology Co., Ltd.; mouse tumor necrosis factor α (TNF-α) enzyme-linked immunosorbent assay kit was obtained from Wuhan Elite Biotechnology Co., Ltd.; mouse interleukin 6 (IL-6) enzyme-linked immunosorbent assay kit was obtained from Wuhan Elite Biotechnology Co., Ltd.; DPPH reagent was obtained from Solarbio; 2,2′-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) was obtained from Sigma.

[0068] Example 1

[0069] Thiouric acid has anti-inflammatory effects and can reduce the levels of inflammatory factors IL-6 and TNF-α.

[0070] Experimental Procedure: 2 million RAW264.7 cells (P9) were seeded into 6-well plates per well. After 24 hours, 2 mL of the following samples were added: positive control sample (100 μg / mL dexamethasone), experimental group 1 sample (100 μg / mL thiouric acid), experimental group 2 sample (100 μg / mL vitamin B3), experimental group 3 sample (100 μg / mL caffeine), experimental group 4 sample (100 μg / mL thiouric acid + 100 μg / mL vitamin B3), experimental group 5 sample (100 μg / mL thiouric acid + 100 μg / mL caffeine), or experimental group 6 sample (100 μg / mL vitamin B3 + 100 μg / mL caffeine). After 1.5 hours, except for the control group, 2 μL of 1000 μg / mL LPS was added. Continue culturing for 24 hours, collect the supernatant, centrifuge, and take the supernatant. Dilute the supernatant 200 times for the TNF-α experiment and 50 times for the IL-6 experiment. Then, use the mouse tumor necrosis factor α (TNF-α) enzyme-linked immunosorbent assay kit and the mouse interleukin 6 (IL-6) enzyme-linked immunosorbent assay kit to detect the concentrations of inflammatory factors TNF-α and IL-6, respectively. Within 15 minutes of adding the stop solution, detect the absorbance at 450 nm (three replicates per group).

[0071] Experimental results: as shown in Table 2 and Figure 1 As shown, thiouracil significantly inhibited TNF-α protein levels, with an inhibition rate of 39.43% (P < 0.0001, P < 0.05 was considered statistically significant). Thiouracil showed a synergistic effect with vitamin B3 (CI > 1.15). Thiouracil also showed a synergistic effect with caffeine (CI > 1.15). Vitamin B3 and caffeine did not show a synergistic effect (CI < 1.15).

[0072] The inhibitory effect of thiouric acid combined with vitamin B3 on TNF-α was significantly better than that of thiouric acid or vitamin B3 alone. The inhibitory effect of "thiouric acid + vitamin B3" on TNF-α was significantly higher than that of thiouric acid (P<0.001); the inhibitory effect of "thiouric acid + vitamin B3" on TNF-α was significantly higher than that of vitamin B3 (P<0.0001) (P<0.05 is considered statistically significant).

[0073] The combined use of thiouric acid and caffeine significantly enhanced the inhibitory effect on TNF-α compared to either thiouric acid or caffeine alone. The inhibitory effect of thiouric acid + caffeine on TNF-α was significantly higher than that of thiouric acid alone (P<0.001); the inhibitory effect of thiouric acid + caffeine on TNF-α was significantly higher than that of caffeine alone (P<0.001) (P<0.05 was considered statistically significant).

[0074] Table 2: Ability to inhibit TNF-α

[0075]

[0076] As shown in Table 3 and Figure 2 As shown, thiouracil significantly inhibited IL-6 protein levels, with an inhibition rate of 51.96% (P < 0.0001, where P < 0.05 was considered statistically significant). Thiouracil showed a synergistic effect with vitamin B3 (CI > 1.15). Thiouracil also showed a synergistic effect with caffeine (CI > 1.15). Vitamin B3 and caffeine did not show a synergistic effect (CI < 1.15).

[0077] The inhibitory effect of thiouric acid combined with vitamin B3 on IL-6 was significantly better than that of thiouric acid or vitamin B3 alone. The inhibitory effect of "thiouric acid + vitamin B3" on IL-6 was significantly higher than that of thiouric acid (P<0.01); the inhibitory effect of "thiouric acid + vitamin B3" on IL-6 was significantly higher than that of vitamin B3 (P<0.0001) (P<0.05 is considered statistically significant).

[0078] The combined use of thiouric acid and caffeine significantly enhanced the inhibitory effect on IL-6 compared to either thiouric acid or caffeine alone. The inhibitory effect of thiouric acid + caffeine on IL-6 was significantly higher than that of thiouric acid alone (P<0.01); the inhibitory effect of thiouric acid + caffeine on IL-6 was significantly higher than that of caffeine alone (P<0.0001) (P<0.05 was considered statistically significant).

[0079] Table 3: Ability to Inhibit IL-6

[0080]

[0081] Lipopolysaccharide (LPS) can promote inflammation, producing a large number of inflammatory factors and promoting the expression of related inflammatory proteins. LPS is commonly used to activate mouse leukemia macrophages (RAW264.7) as a cellular inflammation model. Compared with the negative control group, thiouric acid significantly reduced the concentrations of IL-6 and TNF-α (P<0.05). Synergistic effects were calculated using the King's method (CI values ​​between 0.85 and 1.15 were additive; between 1.15 and 20 were enhancing; >20 was significantly enhancing; CI values ​​between 0.85 and 0.55 were antagonistic; <0.55 was significantly antagonistic). Thiouric acid and vitamin B3 showed synergistic effects; thiouric acid and caffeine also showed synergistic effects.

[0082] Example 2:

[0083] Thiouronic acid has antioxidant properties and can scavenge ABTS and DPPH free radicals.

[0084] Experimental procedure:

[0085] Add 140 μL of DPPH solution to a 96-well plate. Then add 140 μL of the following: positive control sample (1000 μg / mL ergothioneine), experimental group 1 sample (1000 μg / mL thiouric acid), experimental group 2 sample (1000 μg / mL vitamin B3), experimental group 3 sample (1000 μg / mL caffeine), experimental group 4 sample (1000 μg / mL thiouric acid + 1000 μg / mL vitamin B3), experimental group 5 sample (1000 μg / mL thiouric acid + 1000 μg / mL caffeine), or experimental group 6 sample (1000 μg / mL vitamin B3 + 1000 μg / mL caffeine). Incubate in the dark for 5 min, and measure the absorbance at 420 nm (three replicates per group).

[0086] Experimental results: as shown in Table 4 and Figure 3 As shown, thiouracil can scavenge DPPH free radicals with a scavenging rate of 63.17% (P < 0.0001, P < 0.05 is considered statistically significant). Thiouracil showed a synergistic effect with vitamin B3 (CI = 1.17, CI > 1.15). Thiouracil also showed a synergistic effect with caffeine (CI = 1.18, CI > 1.15). Vitamin B3 and caffeine did not show a synergistic effect (CI = 0.91, CI < 1.15).

[0087] The combined use of thiouric acid and vitamin B3 significantly enhanced the ability of thiouric acid to scavenge DPPH free radicals compared to either thiouric acid or vitamin B3 alone. The DPPH free radical scavenging ability of "thiouric acid + vitamin B3" was significantly higher than that of thiouric acid alone (P<0.0001); the DPPH free radical scavenging ability of "thiouric acid + vitamin B3" was significantly higher than that of vitamin B3 alone (P<0.0001) (P<0.05 was considered statistically significant).

[0088] The combined use of thiouric acid and caffeine significantly enhanced the ability of thiouric acid to scavenge DPPH free radicals compared to either thiouric acid or caffeine alone. The DPPH free radical scavenging ability of "thiouric acid + caffeine" was significantly higher than that of thiouric acid alone (P<0.0001); the DPPH free radical scavenging ability of "thiouric acid + caffeine" was significantly higher than that of caffeine alone (P<0.0001) (P<0.05 was considered statistically significant).

[0089] Table 4: Ability to scavenge DPPH free radicals

[0090]

[0091]

[0092] Experimental procedure:

[0093] An ABTS working solution was prepared by mixing equal volumes of 2.45 mM persulfate and 7 mM ABTS. The reaction was carried out in the dark for 16 hours. The resulting free radical solution was then diluted with PBS buffer (pH 7.4) until the absorbance of the ABTS working solution at 420 nm was within the range of 0.70 ± 0.05. 140 μL of the ABTS solution was added to a 96-well plate. Then, add 140 μL of the positive control sample (1000 μg / mL ergothioneine), or the experimental group 1 sample (1000 μg / mL thiouric acid), or the experimental group 2 sample (1000 μg / mL vitamin B3), or the experimental group 3 sample (1000 μg / mL caffeine), or the experimental group 4 sample (1000 μg / mL thiouric acid + 1000 μg / mL vitamin B3), or the experimental group 5 sample (1000 μg / mL thiouric acid + 1000 μg / mL caffeine), or the experimental group 6 sample (1000 μg / mL vitamin B3 + 1000 μg / mL caffeine). React in the dark for 5 min, and measure the absorbance at 420 nm (three replicates for each group).

[0094] Experimental results:

[0095] As shown in Table 5 and Figure 4As shown, thiouric acid can scavenge ABTS free radicals with a scavenging rate of 63.17% (P < 0.0001, P < 0.05 is considered statistically significant). Thiouric acid has a synergistic effect with vitamin B3, with a CI of 1.26 (CI > 1.15). Thiouric acid also has a synergistic effect with caffeine, with a CI of 1.19 (CI > 1.15). Vitamin B3 and caffeine do not have a synergistic effect, with a CI of 1.06 (CI < 1.15).

[0096] The combined use of thiouric acid and vitamin B3 significantly enhanced the ability of thiouric acid to scavenge ABTS free radicals compared to either thiouric acid or vitamin B3 alone. The ABTS free radical scavenging ability of "thiouric acid + vitamin B3" was significantly higher than that of thiouric acid alone (P<0.0001); the ABTS free radical scavenging ability of "thiouric acid + vitamin B3" was significantly higher than that of vitamin B3 alone (P<0.0001) (P<0.05 was considered statistically significant).

[0097] The combined use of thiouric acid and caffeine significantly enhanced the ability of thiouric acid to scavenge ABTS free radicals compared to either thiouric acid or caffeine alone. The combination of thiouric acid and caffeine significantly enhanced the ability of thiouric acid to scavenge ABTS free radicals compared to thiouric acid alone (P<0.0001); the combination of thiouric acid and caffeine also significantly enhanced the ability of thiouric acid to scavenge ABTS free radicals compared to caffeine alone (P<0.0001) (P<0.05 was considered statistically significant).

[0098] Table 5: Ability to scavenge ABTS free radicals

[0099]

[0100] DPPH and ABTS are two types of free radicals. DPPH has a maximum absorption wavelength at 517 nm; ABTS has a maximum dilution wavelength at 420 nm. Antioxidants can scavenge both DPPH and ABTS free radicals, resulting in a decrease in absorbance. Therefore, DPPH and ABTS free radical scavenging experiments are commonly used to assess the antioxidant capacity of compounds. Compared with the Control group, thiouric acid scavenged both DPPH and ABTS free radicals, and this was statistically significant (P<0.05). Synergistic effects were calculated using the King's method (CI values ​​between 0.85 and 1.15 indicate simple addition; between 1.15 and 20 indicate enhancement; >20 indicates significant enhancement; CI values ​​between 0.85 and 0.55 indicate antagonism; <0.55 indicates significant antagonism). Thiouric acid and vitamin B3 have a synergistic effect; thiouric acid and caffeine have a synergistic effect.

[0101] Example 3: Usage Method

[0102] Take each component according to the dosage in Table 6, including the following steps:

[0103] Phase B was pre-dissolved by heating (60℃), and phase C was swollen by heating in a water bath (60℃). Phase C was then added to phase B and homogenized for 3 minutes. Phase D was then added and homogenized for 3 minutes. After homogenization, the mixture was stirred and cooled to 60℃. The completely dissolved phase A was then added and the mixture was cooled to room temperature and stirred until homogeneous.

[0104] Table 6 Soothing and Antioxidant Emulsion Formula

[0105]

[0106]

[0107] Ten volunteers with sensitive skin were selected and applied the soothing, anti-glycation, and brightening lotion from Example 3 twice a day after cleansing their faces in the morning and evening for 28 consecutive days.

[0108] Subjective evaluations: After 28 days of use, subjective evaluations were conducted by the subjects, including their assessment of improvements in four areas: skin feel, pain relief, redness reduction, and brightening. Evaluation criteria were categorized into three levels: good, fair, and poor. Additionally, during follow-up visits for product safety evaluation, adverse reaction assessments were conducted, inquiring about symptoms such as erythema and stinging on the face. If these symptoms occurred, a patch test was performed to assess the correlation with the test product.

[0109] Subjective evaluations were conducted after 28 days of use, including improvements in four aspects: skin feel, pain relief, redness reduction, and brightening. See Table 7 for details.

[0110]

[0111] Throughout the product safety assessment study, no adverse reactions were observed in the subjects, confirming that the soothing and antioxidant emulsion has a high level of safety.

[0112] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A composition, characterized in that, This includes one of the vitamin B3 or caffeine, along with thiouric acid; The mass ratio of one of the vitamin B3 or caffeine to thiouric acid is 1:2 to 2:

1.

2. The composition according to claim 1, characterized in that, The mass ratio of one of the vitamin B3 or caffeine to thiouric acid is 1:

1.

3. The use of the composition according to claim 1 or 2 in the preparation of soothing, anti-inflammatory and antioxidant products.

4. The application according to claim 3, characterized in that, The antioxidants include scavenging ABTS free radicals and DPPH free radicals; The anti-inflammatory effects include reducing the levels of inflammatory factors IL-6 and TNF-α; The soothing effects include relieving pain, reducing redness, and / or brightening.

5. A soothing, anti-inflammatory, and antioxidant product, characterized in that, The raw materials include the soothing, anti-inflammatory, and antioxidant composition as described in claim 1 or 2.

6. The soothing, anti-inflammatory, and antioxidant product according to claim 5, characterized in that, The product is a cosmetic, and the dosage form of the cosmetic is liquid, lotion, emulsion, powder or cream.

7. A soothing, anti-inflammatory, and antioxidant emulsion, characterized in that, The ingredients include the following parts by weight:

8. The soothing, anti-inflammatory, and antioxidant emulsion according to claim 7, characterized in that, The preservative comprises p-hydroxyacetophenone and 1,2-hexanediol; the mass ratio of p-hydroxyacetophenone to 1,2-hexanediol is 0.1:0.

2. The moisturizers include glycerin, trehalose, and jojoba seed oil; the mass ratio of glycerin, trehalose, and jojoba seed oil is 3:0.5:0.

1. The setting agent is xanthan gum; The stabilizer / antifreeze is dipropylene glycol; the antioxidant is vitamin E; and the emulsifier is cetearyl glucoside.

9. A method for preparing the soothing, anti-inflammatory, and antioxidant emulsion according to any one of claims 7-8, characterized in that, Includes the following steps: S1) The preservative is dissolved to obtain phase A; One of vitamin B3 or caffeine, thiouric acid, water, humectant, and fixative are pre-dissolved by heating to obtain phase B; After the stabilizer / antifreeze and antioxidant swell upon heating, phase C is obtained; S2) Add phase C to phase B and homogenize, then add emulsifier and homogenize again. After cooling, add phase A, cool and stir to obtain the final product.

10. The preparation method according to claim 9, characterized in that, The temperature for pre-dissolving in step S1) is 55–65°C; the temperature for swelling in step S2 is 55–65°C. The homogenization time for S2) is 2 to 4 minutes; after homogenization, the mixture is stirred and cooled to 55 to 65°C.

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