A skin care composition having moisturizing and soothing efficacy and a method of preparing the same

Abstract

The present application belongs to the technical field of cosmetics, and relates to a skin care composition with moisturizing and soothing effects and a preparation method thereof. The skin care composition contains the following raw materials in parts by weight: trehalose 22-28 parts, tremella extract 18-22 parts, Saccharomyces bitype fermentation product filtrate 10-15 parts, gynura leaf extract 12-18 parts, oat peptide 3-5 parts, Astragalus membranaceus extract 6-8 parts, and spilanthes acmella extract 4-6 parts. The present application uses the synergistic combination of tremella extract, gynura leaf extract, Astragalus membranaceus extract and spilanthes acmella extract as the active core, and is supplemented with trehalose, oat peptide and Saccharomyces bitype fermentation product filtrate as the efficacy enhancing component, so that the skin moisturizing and soothing dual effects are realized through the combined action of various active ingredients.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to a skin care composition with moisturizing and soothing effects and its preparation method. Background Technology

[0002] The outermost layer of human skin, the stratum corneum, acts as a protective barrier against external changes and harmful substances. The stratum corneum constantly absorbs moisture from the body and also from the external environment; therefore, changes in the relative humidity of the external environment have a significant impact on the moisture content of the stratum corneum.

[0003] The most basic function that skincare cosmetics should possess is moisturizing. Their basic components are water, humectants, and oils. Water directly softens the skin's stratum corneum, humectants more effectively retain moisture, and they defend against and inhibit hardening after dryness. Oils prolong the retention and softening effects of water and humectants.

[0004] Moisturizers can be broadly classified into two categories based on their moisturizing mechanism. One category consists of moisturizers that work by absorbing and retaining water, such as glycerin, sorbitol, and hyaluronic acid. The other category consists of moisturizers that have a structure similar to the components of the stratum corneum, making them easily penetrate the skin and form a network structure of secondary bound water or associated water with the free water in the stratum corneum, thus preventing water loss and achieving the purpose of moisturizing. Examples of moisturizers include ceramides and collagen.

[0005] Sensitive skin is a common skin problem, characterized by subjective discomfort such as burning, stinging, itching, and tightness in response to external stimuli, such as temperature changes, cosmetics, ultraviolet rays, and pollutants. It may or may not be accompanied by visible skin damage. In recent years, due to factors such as increased environmental pollution, increased life stress, increased frequency of cosmetic use, and over-cleansing, the incidence of sensitive skin has been rising year by year. Therefore, developing skincare products with soothing effects has become a research hotspot in the cosmetics field.

[0006] Currently, the most common soothing ingredients in commercially available soothing skincare products are mainly chemically synthesized active ingredients, such as neuropeptides, bisabolol, dipotassium glycyrrhizate, and 4-tert-butylcyclohexanol. Although chemically synthesized soothing ingredients have clear targets and direct effects, long-term use may lead to risks such as decreased skin tolerance and increased irritation. Some ingredients may even cause contact dermatitis.

[0007] The soothing effects of single plant extracts are often insufficient, and natural plant ingredients generally suffer from complex active ingredients and unclear mechanisms of action. This poses challenges in terms of formulation compatibility and efficacy sustainability, limiting their widespread application in skincare products. Therefore, developing a skincare composition that retains the natural advantages of plant extracts while possessing clear moisturizing and soothing effects and high safety has become a pressing technical problem to be solved in this field. Summary of the Invention

[0008] In view of the shortcomings of the prior art described above, the purpose of this invention is to provide a skin care composition with moisturizing and soothing effects and a method for preparing the same, in order to solve the problems in the prior art.

[0009] To achieve the above objectives, the present invention adopts the following technical solution: This invention provides a skin care composition with moisturizing and soothing effects, the skin care composition containing the following raw materials in parts by weight: trehalose 22-28 parts, tremella extract 18-22 parts, bifida ferment lysate filtrate 10-15 parts, centella asiatica leaf extract 12-18 parts, oat peptide 3-5 parts, astragalus membranaceus extract 6-8 parts, and purslane extract 4-6 parts.

[0010] In one embodiment of this disclosure, the skin care composition contains the following ingredients in parts by weight: 25 parts trehalose, 20 parts tremella extract, 12 parts bifida ferment lysate filtrate, 15 parts centella asiatica leaf extract, 4 parts oat peptide, 7 parts astragalus membranaceus extract, and 5 parts purslane extract.

[0011] In one embodiment of this disclosure, the skin care composition contains the following ingredients in parts by weight: 22 parts trehalose, 22 parts tremella extract, 10 parts bifida ferment lysate filtrate, 18 parts centella asiatica leaf extract, 5 parts oat peptide, 6 parts astragalus membranaceus extract, and 4 parts purslane extract.

[0012] In one embodiment of this disclosure, the skin care composition contains the following ingredients in parts by weight: 25 parts trehalose, 18 parts tremella extract, 15 parts bifida ferment lysate filtrate, 15 parts centella asiatica leaf extract, 4 parts oat peptide, 7 parts astragalus membranaceus extract, and 5 parts purslane extract.

[0013] This invention provides a skin care product with moisturizing and soothing effects, the active ingredients of which include the skin care composition described in this invention.

[0014] This invention provides a cosmetic with moisturizing and soothing effects, the active ingredients of which include the skin care composition described in this invention.

[0015] This invention provides the application of the above-mentioned skin care composition with moisturizing and soothing effects in the preparation of products with moisturizing effects.

[0016] This invention provides the application of the above-mentioned skin care composition with moisturizing and soothing effects in the preparation of products with soothing effects.

[0017] Furthermore, the product is a skincare product, which includes any one of lotion, face cream, eye cream, face mask, moisturizing spray, body lotion, and hand cream.

[0018] The present invention also provides a method for preparing the above-mentioned skin care composition with moisturizing and soothing effects. The preparation method is as follows: weigh trehalose, tremella extract, oat peptide, bifida ferment filtrate, astragalus extract, purslane extract, and centella asiatica leaf extract in proportion, mix them well, and the skin care composition is obtained.

[0019] This invention uses a synergistic blend of four active ingredients—Tremella fuciformis extract, Centella asiatica leaf extract, Astragalus membranaceus extract, and Portulaca oleracea extract—as its core active ingredient, supplemented with trehalose, oat peptides, and Bifida ferment lysate filtrate as efficacy-enhancing components. Through the synergistic effect of multiple active ingredients, it achieves both skin moisturizing and soothing effects.

[0020] Centella asiatica extract contains asiaticoside and hydroxyasiaticoside, which promote collagen synthesis and inhibit inflammatory factors. Purslane extract is rich in flavonoids, alkaloids, and polysaccharides, offering multiple benefits including anti-allergy, anti-inflammation, and soothing. Astragalus membranaceus extract contains astragalus polysaccharides and flavonoids, which have antioxidant properties and enhance skin immunity. Tremella fuciformis extract contains tremella polysaccharides, which have excellent moisturizing and film-forming properties, helping to repair the skin barrier. Trehalose is a natural sugar with excellent cell-maintaining and biomolecular-weight-active properties. Its moisturizing mechanism involves forming a special protective film that can replace the role of water in cells when the skin loses moisture, protecting skin cells from damage. Oat active peptides have moisturizing and cell metabolism-promoting effects. Furthermore, oats are rich in amino acids, which effectively lock in skin moisture and keep the skin hydrated. Oat peptides, composed of these amino acids, not only retain the natural moisturizing properties of amino acids but also have a smaller molecular structure that allows for faster absorption by the skin, while also providing more comprehensive skincare benefits such as antioxidant effects and promotion of collagen synthesis. Bifida ferment filtrate and lysate have significant moisturizing effects on the skin.

[0021] Compared with the prior art, the present invention has the following advantages: The skin care composition with moisturizing and soothing effects provided by the present invention has components that work together to enhance the effects of hyaluronidase inhibition, resulting in excellent skin soothing effects, and at the same time, excellent skin moisturizing effects.

[0022] The method for preparing a skincare composition with moisturizing and soothing effects provided by this invention is simple, easy to industrialize, gentle and non-irritating, and highly safe. It can be added as a raw material to skincare and cosmetic products. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in this invention or the prior art, the accompanying drawings used in the specific embodiments will be briefly described below.

[0024] Figure 1 Comparison of humidification rate at RH=43% for 12 hours, compared with Example 2 group. P < 0.05 P < 0.01.

[0025] Figure 2 Comparison of humidification rate at RH=85% for 12 hours, compared with Example 2 group. P < 0.05 P < 0.01.

[0026] Figure 3 The effect on hyaluronidase inhibition ability, compared with Example 2 group, P < 0.05 P < 0.01. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below.

[0028] For simplicity, this paper only explicitly discloses some numerical ranges. However, any lower limit can be combined with any upper limit to form an unspecified range; and any lower limit can be combined with other lower limits to form an unspecified range, just as any upper limit can be combined with any other upper limit to form an unspecified range. Furthermore, although not explicitly stated, every point or individual value between the endpoints of a range is included within that range. Therefore, each point or individual value can serve as its own lower or upper limit and be combined with any other point or individual value, or with other lower or upper limits, to form an unspecified range.

[0029] In the description of this article, it should be noted that, unless otherwise stated, "above" and "below" include the number itself, and "several" in "one or more" means two or more.

[0030] The above description of the invention is not intended to describe every disclosed embodiment or implementation of the invention. Instead, the following description provides more specific examples of exemplary embodiments. Throughout this application, guidance is provided through a series of embodiments that can be used in various combinations. In each example, the examples are listed only as representative groups and should not be construed as exhaustive.

[0031] Unless otherwise specified in the examples, the procedures shall be performed under standard conditions or conditions recommended by the manufacturer. All raw materials and instruments used, unless otherwise specified by the manufacturer, are commercially available standard products.

[0032] The trehalose described in this invention is a commercially available cosmetic-grade raw material with CAS number 99-20-7.

[0033] The Bifida ferment filtrate of the present invention, also known as Bifidobacterium longum ferment filtrate, can be prepared according to the method in Chinese Patent CN202410984482.2.

[0034] The purslane extract of the present invention can be prepared according to the method in Chinese Patent CN201610100815.6.

[0035] The oat peptides described in this invention are commercially available cosmetic-grade raw materials, with CAS number 151661-87-9. The oat peptides described in this invention were purchased from Shaanxi Baichuan Kangze Biotechnology Co., Ltd.

[0036] The tremella polysaccharide described in this invention is a commercially available cosmetic-grade raw material with CAS number 9075-53-0.

[0037] The Tremella fuciformis extract, Centella asiatica leaf extract, and Astragalus membranaceus extract of this invention are corresponding water extracts. The preparation method is as follows: Tremella fuciformis, Centella asiatica leaf, and Astragalus membranaceus are taken separately, and 10 times (v / w) of purified water is added. The mixture is heated and refluxed twice, each time for 0.5 h. The extracts are combined, filtered, and the filtrate is concentrated under reduced pressure to a clear extract with a relative density of 1.05 at 60°C to obtain Tremella fuciformis extract, Centella asiatica leaf extract, and Astragalus membranaceus extract, respectively.

[0038] The preparation method of the aloe vera extract of the present invention is as follows: take aloe vera and add 10 times (v / w) of purified water, heat and reflux to extract twice, each time for 0.5 h, combine the extracts, filter, concentrate the filtrate under reduced pressure to a clear extract with a relative density of 1.05 at 60℃, and obtain aloe vera extract.

[0039] The preparation method of the golden chamomile extract of the present invention is as follows: take golden chamomile and add 10 times (v / w) of purified water, heat and reflux to extract twice, each time for 0.5h, combine the extracts, filter, and concentrate the filtrate under reduced pressure to a clear extract with a relative density of 1.05 at 60℃ to obtain the golden chamomile extract.

[0040] The preparation method of the ginseng extract of the present invention is as follows: take ginseng and add 10 times (v / w) of purified water, heat and reflux to extract twice, each time for 0.5h, combine the extracts, filter, and concentrate the filtrate under reduced pressure to a clear extract with a relative density of 1.05 at 60℃ to obtain the ginseng extract.

[0041] The preparation method of the skin care composition in Examples 1-7 is as follows: weigh trehalose, tremella extract, oat peptide, bifida ferment lysate filtrate, astragalus membranaceus extract, purslane extract, and centella asiatica leaf extract according to the proportions, mix them well, and the skin care composition is obtained.

[0042] Example 1 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: 25 parts trehalose, 20 parts tremella extract, 12 parts bifida ferment lysate filtrate, 15 parts centella asiatica leaf extract, 4 parts oat peptide, 7 parts astragalus membranaceus extract, and 5 parts purslane extract.

[0043] Example 2 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: 22 parts trehalose, 22 parts tremella extract, 10 parts bifida ferment lysate filtrate, 18 parts centella asiatica leaf extract, 5 parts oat peptide, 6 parts astragalus membranaceus extract, and 4 parts purslane extract.

[0044] Example 3 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: 25 parts trehalose, 18 parts tremella extract, 15 parts bifida ferment lysate filtrate, 15 parts centella asiatica leaf extract, 4 parts oat peptide, 7 parts astragalus membranaceus extract, and 5 parts purslane extract.

[0045] Example 4 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: 22 parts trehalose, 18 parts tremella extract, 10 parts bifida ferment lysate filtrate, 12 parts centella asiatica leaf extract, 3 parts oat peptide, 8 parts astragalus membranaceus extract, and 6 parts purslane extract.

[0046] Example 5 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: 28 parts trehalose, 22 parts tremella extract, 12 parts bifida ferment lysate filtrate, 15 parts centella asiatica leaf extract, 5 parts oat peptide, 8 parts astragalus membranaceus extract, and 5 parts purslane extract.

[0047] Example 6 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: trehalose 25 parts, tremella extract 22 parts, bifida ferment lysate filtrate 15 parts, centella asiatica leaf extract 12 parts, oat peptide 3 parts, astragalus membranaceus extract 6 parts, and purslane extract 6 parts.

[0048] Example 7 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: 28 parts trehalose, 20 parts tremella extract, 12 parts bifida ferment lysate filtrate, 15 parts centella asiatica leaf extract, 4 parts oat peptide, 7 parts astragalus membranaceus extract, and 5 parts purslane extract.

[0049] Comparative Example 1 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: 25 parts trehalose, 20 parts tremella polysaccharide, 12 parts bifida ferment lysate filtrate, 15 parts centella asiatica leaf extract, 4 parts oat peptide, 7 parts astragalus membranaceus extract, and 5 parts purslane extract.

[0050] Compared with Example 1, the difference is that Tremella polysaccharide is used instead of Tremella extract, while the other components and amounts remain unchanged.

[0051] The preparation method is the same as in Example 1.

[0052] Comparative Example 2 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: 25 parts trehalose, 20 parts tremella extract, 12 parts bifida ferment lysate filtrate, 15 parts centella asiatica leaf extract, 4 parts oat peptide, 7 parts astragalus membranaceus extract, and 5 parts aloe vera extract.

[0053] Compared with Example 1, the difference is that aloe vera extract is used instead of purslane extract, while the other components and amounts remain unchanged.

[0054] The preparation method is the same as in Example 1.

[0055] Comparative Example 3 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: 25 parts trehalose, 20 parts tremella extract, 12 parts bifida ferment lysate filtrate, 15 parts golden chamomile extract, 4 parts oat peptides, 7 parts Astragalus membranaceus extract, and 5 parts purslane extract.

[0056] Compared with Example 1, the difference is that golden chamomile extract is used instead of centella asiatica leaf extract, while the other components and amounts remain unchanged.

[0057] The preparation method is the same as in Example 1.

[0058] Comparative Example 4 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: 25 parts trehalose, 20 parts tremella extract, 12 parts bifida ferment lysate filtrate, 15 parts centella asiatica leaf extract, 4 parts oat peptide, 7 parts ginseng extract, and 5 parts purslane extract.

[0059] Compared with Example 1, the difference is that ginseng extract is used instead of Astragalus membranaceus extract, while the other components and their amounts remain unchanged.

[0060] The preparation method is the same as in Example 1.

[0061] Comparative Example 5 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: trehalose 31 parts, tremella extract 26 parts, centella asiatica leaf extract 15 parts, oat peptide 4 parts, astragalus membranaceus extract 7 parts, and purslane extract 5 parts.

[0062] Compared with Example 1, the difference is that it does not contain Bifida ferment filtrate, but its amount is increased to trehalose and tremella extract.

[0063] The preparation method is the same as in Example 1.

[0064] Comparative Example 6 A skincare composition with moisturizing and soothing effects contains the following components in parts by weight: 30 parts of Tremella fuciformis extract, 27 parts of Bifida ferment lysate filtrate, 15 parts of Centella asiatica leaf extract, 4 parts of oat peptides, 7 parts of Astragalus membranaceus extract, and 5 parts of Portulaca oleracea extract.

[0065] Compared with Example 1, the difference is that it does not contain trehalose, and its amount is increased in the Tremella fuciformis extract and Bifida ferment filtrate.

[0066] The preparation method is the same as in Example 1.

[0067] Comparative Example 7 Chinese Patent CN 202110200131.4 Example 1: Cosmetic composition, wherein the cosmetic composition comprises 30 parts of Bifida Ferment Lysate filtrate, 3 parts of silk protein extract, 4 parts of purslane extract, 4 parts of biosaccharide gum-1, 0.7 parts of sodium hyaluronate, 1.4 parts of ceramide 3, and 3 parts of Lactobacillus / soy milk ferment filtrate.

[0068] Moisturizing efficacy test of the skin care composition of this invention The moisturizing properties of the skincare composition of the present invention were evaluated using the gravimetric method in in vitro physicochemical analysis.

[0069] The relative humidity in the desiccator was controlled at approximately 43% and 85% (RH, 43% and 85%). To better simulate the actual condition of skin, a glass plate measuring 25mm × 75mm was selected. 3M tape was attached to the plate, and 200mg of the skincare composition solutions from Examples 1-3 and Comparative Examples 1-7 were evenly applied to the tape surface. After being placed in the desiccator for a certain period of time (at time intervals of 6 and 12 hours), the plates were weighed, and the moisturizing rate (R) was calculated. R=M1 / M2 × 100% In the formula, M2 represents the moisture weight (g) after placement; M1 represents the moisture weight (g) before placement.

[0070] At least three parallel experiments were conducted. Experimental data are expressed as mean ± standard deviation. Statistical analysis was performed using the ANOVA test in GraphPad Prism 8 software. p < 0.05 was considered statistically significant.

[0071] The results are shown in Table 1. Figure 1 , Figure 2 .

[0072] At RH=43%, the moisturizing rates of the skin care compositions of Examples 1-3 with a mass fraction of 10% were 94.87%±1.19%, 93.70%±2.66%, and 92.83%±2.43% at 12h, respectively.

[0073] The moisturizing effects of the skin care compositions in Examples 1-3 of this invention are significantly better than those in Comparative Examples 1-7.

[0074] At RH=85%, after 12 hours, the moisturizing rates of the skin care compositions of Examples 1-3 with a mass fraction of 10% were 97.70%±2.27%, 96.17%±1.76%, and 96.23%±1.93%, respectively.

[0075] The moisturizing effects of the skin care compositions in Examples 1-3 of this invention are significantly better than those in Comparative Examples 1-7.

[0076] The skincare composition of this invention has good moisturizing effects.

[0077] Table 1 Comparison of moisturizing activities at 12h Compared with Example 2 group, P < 0.05 P < 0.01.

[0078] The results of Comparative Examples 1-6 show that, under the premise of the same total amount of skin care composition, removing any ingredient or replacing it with another ingredient will lead to a significant decrease in moisturizing rate. This indicates that there is a clear synergistic effect among the ingredients in the skin care composition of the present invention, and its overall inhibitory effect is significantly better than the simple sum of the effects of individual components.

[0079] The results of Examples 1-3 and Comparative Example 7 show that, under the premise of the same total amount of composition, the moisturizing effect of the skin care composition of Examples 1-3 of the present invention is significantly better than that of the composition of Comparative Example 7.

[0080] Hyaluronic acid, a major component of the extracellular matrix and intercellular matrix, plays a crucial role in skin elasticity, cell adhesion, wound healing, and angiogenesis. Studies show that hyaluronic acid is also closely related to allergic reactions and is strongly associated with skin sensitivity. Hyaluronidase, a proteolytic enzyme that specifically breaks down hyaluronic acid, is frequently used in allergy-related research. Common allergy symptoms such as redness, swelling, itching, and pain are primarily caused by histamine, and antihistamines are currently the most commonly used medications in clinical practice. Research shows a strong correlation between hyaluronidase activity inhibition and mast cell histamine release inhibition; active ingredients that inhibit histamine often also exhibit significant hyaluronidase inhibition activity. Therefore, hyaluronidase, as a participant in type 2 hypersensitivity reactions, is often used to explore in vitro anti-allergic active ingredients.

[0081] The anti-allergic activity is indicated by the hyaluronidase inhibition rate; the higher the hyaluronidase inhibition rate, the stronger the anti-allergic activity.

[0082] This study used a hyaluronidase inhibition experiment to explore the soothing effects of the skincare composition of the present invention.

[0083] Anti-allergic efficacy test of the skin care composition of this invention Take 0.1 mL of 0.25 mmol / L CaCl2 solution and 0.5 mL of hyaluronidase solution, and incubate at 37°C for 20 min. Add 0.5 mL of 5% (w / w) skin care composition solution from Examples 1-3 and Comparative Examples 1-7, and continue incubation at 37°C for 20 min. Add 0.5 mL of sodium hyaluronate solution and incubate at 37°C for 30 min, then let stand at room temperature for 5 min. Add 0.1 mL of 0.4 mol / L NaOH solution and 0.5 mL of acetylacetone solution, heat in a boiling water bath for 15 min, and immediately cool with ice water for 5 min. Add 1.0 mL of Ehrlich reagent and dilute with 3.0 mL of anhydrous ethanol, let stand for 20 min for color development, and measure the absorbance value using a spectrophotometer. Dipotassium glycyrrhizate at a concentration of 2 mg / mL was used as a positive control group.

[0084] Calculation of hyaluronidase inhibition rate Hyaluronidase inhibition rate (%) = [(AB) - (CD)] / (AB) × 100%.

[0085] In the formula: A - ABS value of the control solution (using acetate buffer solution instead of the sample solution). B - ABS value of the control blank solution (using acetate buffer solution instead of sample solution and enzyme solution). C-Sample solution ABS value D - ABS value of blank sample solution (using acetate buffer solution instead of enzyme solution) During the experiment, the wavelength of the A group samples was first scanned in the range of 450~700nm to determine the maximum absorption wavelength. Then, using deionized water as a reference, the ABS values ​​of the relevant groups were measured at the maximum absorption wavelength.

[0086] At least three parallel experiments were conducted. Experimental data are expressed as mean ± standard deviation. Statistical analysis was performed using the ANOVA test in GraphPad Prism 8 software. p < 0.05 was considered statistically significant.

[0087] The results are shown in Table 2. Figure 3 The skincare composition of this invention has a significant inhibitory effect on hyaluronidase, suggesting that the skincare composition of this invention has good soothing and anti-allergic effects.

[0088] The skin care compositions of Examples 1-3 of this invention have significantly better soothing and anti-allergic effects than those of Comparative Examples 1-7.

[0089] Table 2 Effects on hyaluronidase inhibition Compared with Example 2 group, P < 0.05 P < 0.01.

[0090] The results of Comparative Examples 1-6 show that, under the premise of the same total amount of skin care composition, removing any ingredient or replacing it with another ingredient leads to a significant decrease in the inhibition rate of hyaluronidase. This indicates that there is a clear synergistic effect among the ingredients in the skin care composition of the present invention, and its overall inhibitory effect is significantly better than the simple sum of the effects of the individual components.

[0091] The results of Examples 1-3 and Comparative Example 7 show that, under the premise of the same total amount of composition, the soothing and anti-allergic effects of the skin care compositions of Examples 1-3 of the present invention are significantly better than those of Comparative Example 7.

[0092] Safety testing of the skin care composition of the present invention The safety of the skin care composition of Example 1 of the present invention was determined by conducting multiple skin irritation tests.

[0093] 1) Animals were acclimatized to the experimental animal facility for 3 days prior to the experiment to ensure they were healthy and had no broken skin. 2) Approximately 24 hours before the experiment, the hair on both sides of the spine on the back of the experimental animals was shaved off, with a shaving area of ​​approximately 3cm × 3cm on each side, taking care not to damage the skin. The application area was 2.5cm × 2.5cm. 3) After weighing the animals the following day, 0.5mL of the skincare composition from Example 1 was evenly applied to the left side of the subject's skin, then covered with two layers of gauze and one layer of cellophane, and secured with non-irritating adhesive tape and bandages. The right side of the skin served as a blank control, without any substance applied. Application was performed once daily for 14 consecutive hours. 4) Observation: Starting from the second day, hair was removed before each application, and any remaining test substance was washed off with warm water. Observation results were recorded 1 hour later. Skin irritation testing was based on multiple skin irritation tests according to the "Cosmetic Safety Technical Specifications". Erythema was graded from 0 to 4 (no erythema to severe erythema), and edema was graded from 0 to 4 (no edema to severe edema). Calculate the average daily score for each group, and determine the level of skin irritation intensity based on the average total score over 14 days.

[0094] Table 3 Results of repeated skin irritation tests on rabbits The results are shown in Table 3. The skin care composition of Example 1 is highly safe and has no skin irritation.

[0095] Content not described in detail in this application specification is common knowledge to those skilled in the art. The foregoing description illustrates and describes several preferred embodiments of this application; however, as mentioned above, it should be understood that this application is not limited to the forms disclosed herein and should not be construed as excluding other embodiments. It can be used in various other combinations, modifications, and environments, and can be altered within the scope of the inventive concept described herein through the foregoing teachings or techniques or knowledge in related fields. Any modifications and variations made by those skilled in the art that do not depart from the spirit and scope of this application should be within the protection scope of the appended claims.

Claims

1. A skincare composition with moisturizing and soothing effects, characterized in that, The skincare composition is made from the following components: 22-28 parts trehalose, 18-22 parts tremella extract, 10-15 parts bifida ferment filtrate, 12-18 parts centella asiatica leaf extract, 3-5 parts oat peptides, 6-8 parts astragalus membranaceus extract, and 4-6 parts purslane extract.

2. The skincare composition with moisturizing and soothing effects according to claim 1, characterized in that, The skincare composition contains the following ingredients in parts by weight: 25 parts trehalose, 20 parts tremella extract, 12 parts bifida ferment lysate filtrate, 15 parts centella asiatica leaf extract, 4 parts oat peptide, 7 parts astragalus membranaceus extract, and 5 parts purslane extract.

3. The skincare composition with moisturizing and soothing effects according to claim 1, characterized in that, The skincare composition contains the following ingredients in parts by weight: 22 parts trehalose, 22 parts tremella extract, 10 parts bifida ferment lysate filtrate, 18 parts centella asiatica leaf extract, 5 parts oat peptide, 6 parts astragalus membranaceus extract, and 4 parts purslane extract.

4. The skincare composition with moisturizing and soothing effects according to claim 1, characterized in that, The skincare composition contains the following ingredients in parts by weight: 25 parts trehalose, 18 parts tremella extract, 15 parts bifida ferment lysate filtrate, 15 parts centella asiatica leaf extract, 4 parts oat peptide, 7 parts astragalus membranaceus extract, and 5 parts purslane extract.

5. A skincare product with moisturizing and soothing effects, characterized in that, Its active ingredients include the skincare composition as described in claim 1.

6. A cosmetic product with moisturizing and soothing effects, characterized in that, Its active ingredients include the skincare composition as described in claim 1.

7. The use of a skin care composition having moisturizing and soothing effects as described in any one of claims 1-4 in the preparation of a product having moisturizing effects.

8. The use of a skin care composition having moisturizing and soothing effects as described in any one of claims 1-4 in the preparation of a product having soothing effects.

9. The application according to claim 7 or 8, characterized in that, The product is a skincare product, which includes any one of lotion, face cream, eye cream, face mask, moisturizing spray, body lotion, and hand cream.

10. A method for preparing a skincare composition with moisturizing and soothing effects, characterized in that, Includes the following steps: Weigh out the following ingredients in the specified proportions: trehalose, tremella extract, oat peptides, bifida ferment filtrate, astragalus extract, purslane extract, and centella asiatica leaf extract. Mix them well to obtain the skincare composition.

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