A mild whitening and freckle-removing emulsion and a preparation method thereof

Abstract

This invention belongs to the field of cosmetic technology. Specifically, it discloses a mild whitening and spot-fading lotion and its preparation method. This application incorporates a whitening and spot-fading composition into the mild whitening and spot-fading lotion. Through the synergistic effect of Bifida Ferment Lysate, Albizia Bark Extract, and Cherry Blossom Extract, a multi-pathway whitening and spot-fading system is constructed, which inhibits melanin production at its source, combats glycation and yellowing, provides antioxidant protection, promotes metabolism and fades spots, and repairs and stabilizes the skin barrier. Specifically, Bifida Ferment Lysate mainly inhibits melanin synthesis, accelerates surface pigment metabolism, and fades spots; Cherry Blossom Extract precisely targets tyrosinase, resists UV-induced pigmentation, prevents recurrence of spots, and repairs skin cells; Albizia Bark Extract specifically improves skin glycation-induced yellowing and oxidative dullness, soothes inflammation, and stabilizes skin condition. These three components mutually enhance and complement each other, effectively improving the whitening and spot-fading effect.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a mild whitening and spot-fading lotion and its preparation method. Background Technology

[0002] Age spots, dull skin tone, and yellowing skin are the most common skin problems at present. Their causes are complex and mainly result from the combined effects of multiple factors, including ultraviolet radiation, oxidative stress, glycation, slow skin metabolism, and barrier damage. Among them, ultraviolet radiation can activate melanocytes in the basal layer of the skin, increase the activity of tyrosinase, and promote the conversion of tyrosine into melanin, thus forming sun spots and pigmentation. At the same time, long-term skin oxidation will cause free radical accumulation, exacerbating abnormal pigment deposition, while skin glycation will generate a large number of advanced glycation end products (AGEs), which will damage the collagen structure of the skin, causing dullness and decreased skin transparency. In addition, the epidermal metabolic rate is reduced and melanin is not excreted smoothly, ultimately forming stubborn age spots, uneven skin tone, dullness, roughness, and other problems, which seriously affect the appearance of the skin.

[0003] Currently, there are numerous whitening and spot-fading cosmetics on the market, but most of them use single whitening active ingredients such as melanin-inhibiting, antioxidant, or yellowing-reducing ingredients. Their functions are relatively limited, and they generally suffer from many technical deficiencies. Most products only intervene in a single pathway of melanin synthesis, achieving only simple melanin inhibition and spot fading. They cannot simultaneously address multiple needs such as anti-oxidation, anti-glycation, promoting pigment metabolism, and skin repair. This results in one-sided and slow-acting whitening and spot-fading effects, and poor improvement of complex skin problems such as oxidative dullness, glycation yellowing, UV-induced pigmentation, and the accumulation of old pigments.

[0004] Therefore, this application is submitted. Summary of the Invention

[0005] This invention provides a mild whitening and spot-fading emulsion and its preparation method, wherein the emulsion has excellent whitening and spot-fading effects.

[0006] The present invention solves its technical problem by adopting the following technical solution: A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.01-0.05% glycyrrhizin, 0.02-0.1% chelating agent, 0.1-0.2% thickener, 0.2-0.6% glyceryl glucoside, 0.2-1% Centella asiatica extract, 1-2.5% isopropyl myristate, 1-3% jojoba seed oil, 2-3% whitening and spot-fading composition, 1-4% squalane, 3-5% emulsifier, 6-10% moisturizer, and the balance being water; The whitening and spot-fading composition comprises Bifida ferment filtrate, Albizia julibrissin bark extract, and Cherry blossom extract in a mass ratio of (25~50):(25~40):(25~35).

[0007] As an embodiment of this application, the preparation method of the Albizia julibrissin bark extract is as follows: (1) Add Albizia bark powder, cellulase, β-glucosidase and xylanase to water, and sonicate to hydrolyze to obtain hydrolysate; (2) The enzyme hydrolysate is first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5KD, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 0.5KD to obtain the filtrate; (3) The filtrate was separated by passing it through a macroporous adsorption resin and dried to obtain an extract of Albizia julibrissin bark.

[0008] As an embodiment of this application, the mass ratio of the Albizia bark powder, cellulase, β-glucosidase, xylanase, and water is 1:(0.006~0.012):(0.004~0.008):(0.004~0.008):(8~20).

[0009] As an implementation scheme of this application, the separation of the filtrate by passing it through a macroporous adsorption resin is specifically as follows: the filtrate is passed through the macroporous adsorption resin at a flow rate of 0.8~1.5 BV / h, the elution rate is 0.8~1.5 BV / h, it is first washed with water for 1~2 BV, and then washed with 50~75wt% ethanol solution for 0.8~2 BV, and the ethanol solution eluent is collected.

[0010] As an embodiment of this application, the ultrasonic enzymatic hydrolysis temperature is 42~50℃, the time is 2~10h, and the ultrasonic power is 200~600W.

[0011] As an embodiment of this application, the macroporous adsorption resin is at least one of HDP-600 macroporous resin and HDP-500 macroporous resin.

[0012] As an embodiment of this application, the moisturizer includes at least one selected from 1,4-butanediol, 1,3-propanediol, 1,2-hexanediol, dipropylene glycol, and glycerin; The thickener includes at least one of carbomer, xanthan gum, argan gum, hydroxyethyl cellulose, ammonium acryloyl dimethyl taurate / VP copolymer, and polyacrylate crosspolymer-6.

[0013] As an embodiment of this application, the chelating agent is disodium EDTA.

[0014] As an embodiment of this application, the emulsifier includes polyglycerol-10 oleate and cetearyl alcohol in a mass ratio of 1:(0.4~0.8).

[0015] This application also provides a method for preparing a mild whitening and spot-fading lotion, comprising the following steps: (1) Add thickener, chelating agent, glyceryl glucoside and humectant to water and stir evenly at 70~90℃ to obtain aqueous phase; (2) Isopropyl myristate, jojoba seed oil, squalane, and emulsifier are stirred evenly at 60~90℃ to obtain the oil phase; (3) Add the oil phase to the aqueous phase, homogenize, stir evenly to obtain an emulsion, cool to 25~45℃, add glycyrrhizin, centella asiatica extract and whitening and spot-fading composition, stir evenly to obtain a mild whitening and spot-fading emulsion.

[0016] The beneficial effects of this invention are as follows: This application incorporates a whitening and spot-fading composition into a mild whitening and spot-fading lotion. Through the synergistic effect of Bifida Ferment Lysate, Albizia Bark Extract, and Cherry Blossom Extract, a multi-pathway whitening and spot-fading system is constructed, which inhibits melanin production at its source, combats glycation and yellowing, provides antioxidant protection, promotes metabolism and fades spots, and repairs and stabilizes the skin barrier. Specifically, Bifida Ferment Lysate mainly inhibits melanin synthesis and accelerates surface pigment metabolism, fading spots; Cherry Blossom Extract precisely targets tyrosinase, resisting UV-induced pigmentation and preventing recurrence of spots, while also repairing skin cells; Albizia Bark Extract specifically improves skin glycation-induced yellowing and oxidative dullness, soothes inflammation, and stabilizes skin condition. These three components mutually enhance and complement each other, effectively improving the whitening and spot-fading effect. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] In this invention, the technical features described in an open-ended manner include both closed-ended technical solutions composed of the listed features and open-ended technical solutions that include the listed features.

[0019] In this invention, numerical ranges are involved. Unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0020] In this invention, there are no particular limitations on the specific dispersion and stirring methods.

[0021] Unless otherwise specified, all reagents or instruments used in this invention are commercially available conventional products. Unless otherwise specified, the raw materials used in each comparative example and the parallel experiments of each embodiment are the same commercially available products.

[0022] The raw materials used in the examples and comparative examples are sourced as follows: Glycyrrhizin: Zhongshan Ruisi Biotechnology Co., Ltd.

[0023] Centella asiatica extract: Hunan Xujing Biotechnology.

[0024] Isopropyl myristate: Guangzhou Wanlu Chemical Co., Ltd.

[0025] Jojoba seed oil: Guangzhou Meiyi Biotechnology.

[0026] Bifida ferment filtrate: Guangzhou Yiwan Biotechnology.

[0027] Fine-toothed cherry blossom extract: Guangzhou Xiangtu Import & Export Trade.

[0028] Squalane: Guangzhou Meiyi Biotechnology.

[0029] Nicotinamide: Guangzhou Wanlu Chemical Co., Ltd.

[0030] Licorice extract: Hunan Langlin Biotechnology.

[0031] Rhodiola rosea root extract: Huayang Biotechnology.

[0032] Polyglycerol-10 oleate: Guangzhou Curie Chemical.

[0033] Cetearyl alcohol: Guangzhou Hefu Chemical Co., Ltd.

[0034] This application provides a mild whitening and spot-fading emulsion, comprising the following components by weight percentage: 0.01-0.05% glycyrrhizin, 0.02-0.1% chelating agent, 0.1-0.2% thickener, 0.2-0.6% glyceryl glucoside, 0.2-1% Centella asiatica extract, 1-2.5% isopropyl myristate, 1-3% jojoba seed oil, 2-3% whitening and spot-fading composition, 1-4% squalane, 3-5% emulsifier, 6-10% moisturizer, and the balance being water; The whitening and spot-fading composition comprises Bifida ferment filtrate, Albizia julibrissin bark extract, and Cherry blossom extract in a mass ratio of (25~50):(25~40):(25~35).

[0035] Bifida Ferment Lysate Filtrate (from the "Catalogue of Used Cosmetic Ingredients (2021 Edition)," No. 02161): Rich in amino acids, vitamins, organic acids, and polysaccharides, it exhibits significant inhibitory activity against tyrosinase and strong antioxidant capacity. It blocks melanin production by targeting the upstream tyrosinase catalysis stage of melanin synthesis. Furthermore, this ingredient enhances skin metabolism, promotes cell proliferation, and accelerates the metabolism of keratinocytes that have already produced melanin, achieving a whitening and spot-fading effect from both the basal and superficial layers.

[0036] Albizia julibrissin bark extract (Catalogue of Used Cosmetic Ingredients (2021 Edition), No. 02775): This extract inhibits non-enzymatic glycation reactions, reduces the formation of advanced glycation end products (AGEs), and repairs protein structural damage caused by glycation. It improves skin yellowing and dullness caused by glycation, and also has good antioxidant, anti-inflammatory, and soothing effects, neutralizing free radicals and delaying the skin aging process.

[0037] Cherry blossom extract (Catalogue of Used Cosmetic Ingredients (2021 Edition), No. 06912): It can effectively inhibit tyrosinase activity in a competitive or non-competitive manner, effectively prevent pigment penetration, slow down or prevent the formation of age spots caused by ultraviolet radiation, and at the same time make the skin rosy and fair. It also has excellent antioxidant effects, can scavenge free radicals, prevent DNA oxidative damage, and protect skin cells.

[0038] This application incorporates a whitening and spot-fading composition into a gentle whitening and spot-fading lotion. Through the synergistic effects of Bifida Ferment Lysate, Albizia Bark Extract, and Cherry Blossom Extract, a multi-pathway whitening and spot-fading system is constructed, which inhibits melanin production at its source, combats glycation and yellowing, provides antioxidant protection, promotes metabolism to fade spots, and repairs and stabilizes the skin barrier. Specifically, Bifida Ferment Lysate primarily inhibits melanin synthesis and accelerates surface pigment metabolism, fading spots; Cherry Blossom Extract precisely targets tyrosinase, resisting UV-induced pigmentation and preventing recurrence of spots while repairing skin cells; Albizia Bark Extract specifically improves glycation-induced yellowing and oxidative dullness, soothes inflammation, and stabilizes skin condition. These three components synergistically enhance and complement each other, effectively improving the whitening and spot-fading effect.

[0039] In some embodiments, the method for preparing the Albizia julibrissin bark extract is as follows: (1) Add Albizia bark powder, cellulase, β-glucosidase and xylanase to water, and sonicate to hydrolyze to obtain hydrolysate; (2) The enzyme hydrolysate is first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5KD, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 0.5KD to obtain the filtrate; (3) The filtrate was separated by passing it through a macroporous adsorption resin and dried to obtain an extract of Albizia julibrissin bark.

[0040] The inventors of this application discovered that the main functions of commercially available Albizia julibrissin bark extract are anti-oxidation and repair, while its whitening and spot-fading effects need improvement (i.e., the whitening and spot-fading effects of commercially available Albizia julibrissin bark extract are insufficient). This application creatively uses cellulase, β-glucosidase, and xylanase to ultrasonically enzymatically hydrolyze Albizia julibrissin bark powder, promoting the dissolution of active ingredients with whitening and spot-fading effects. Following ultrafiltration and macroporous resin separation, an Albizia julibrissin bark extract with excellent whitening and spot-fading effects is obtained. This extract can inhibit non-enzymatic glycation reactions, reduce the formation of advanced glycation end products (AGEs), and repair protein structural damage caused by glycation. It improves skin yellowing and dullness caused by glycation, while also possessing good antioxidant, anti-inflammatory, and soothing effects, neutralizing free radicals, and delaying the skin aging process.

[0041] In some embodiments, the mass ratio of the Albizia bark powder, cellulase, β-glucosidase, xylanase, and water is 1:(0.006~0.012):(0.004~0.008):(0.004~0.008):(8~20).

[0042] In some embodiments, separating the filtrate by passing it through a macroporous adsorption resin specifically involves passing the filtrate through the macroporous adsorption resin at a flow rate of 0.8~1.5 BV / h, with an elution rate of 0.8~1.5 BV / h, first washing with water for 1~2 BV, then washing with 50~75wt% ethanol solution for 0.8~2 BV, and collecting the ethanol eluent.

[0043] In some embodiments, the ultrasonic enzymatic hydrolysis is performed at a temperature of 42-50°C for 2-10 hours, with an ultrasonic power of 200-600W.

[0044] In some embodiments, the particle size of the Albizia bark powder is 50-500 mesh.

[0045] In some embodiments, the macroporous adsorption resin is at least one of HDP-600 macroporous resin and HDP-500 macroporous resin.

[0046] In some embodiments, the humectant includes at least one of 1,4-butanediol, 1,3-propanediol, 1,2-hexanediol, dipropylene glycol, and glycerin.

[0047] In some embodiments, the thickener includes at least one of carbomer, xanthan gum, argan gum, hydroxyethyl cellulose, ammonium acryloyl dimethyl taurate / VP copolymer, and polyacrylate crosspolymer-6.

[0048] In some embodiments, the chelating agent is disodium EDTA.

[0049] In some embodiments, the emulsifier comprises polyglycerol-10 oleate and cetearyl alcohol in a mass ratio of 1:(0.4~0.8).

[0050] In some embodiments, the mild whitening and spot-fading lotion may also contain cosmetic-acceptable excipients.

[0051] In one embodiment, the cosmetic excipients include, but are not limited to, at least one of emollients, preservatives, antioxidants, pH adjusters, penetration enhancers, surfactants, fragrances, and pigments.

[0052] In one embodiment, suitable emollients include, but are not limited to, at least one of isododecane, isohexadecane, castor oil, caprylic / capric triglyceride, dimethicone, isononyl isononanoate, diisostearyl malate, hexyl laurate, sweet almond oil, hydrogenated polyisobutylene, octyl dodecyl alcohol, shea butter, dioctyl carbonate, lanolin, dioctyl adipate, and cocoyl caprylate / capric ester.

[0053] In one embodiment, suitable antioxidants include, but are not limited to, at least one of resveratrol, ascorbic acid and its derivatives, pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester, arbutin, tocopherol (vitamin E), carotenoids, and sodium metabisulfite.

[0054] In one embodiment, a suitable pH adjuster includes, but is not limited to, at least one of tromethamine, aminomethylpropanol, arginine, triethanolamine, citric acid, sodium citrate, sodium hydroxide, and potassium hydroxide.

[0055] In one embodiment, suitable colorants include, but are not limited to, white, black, yellow, blue, green, pink, red, orange, purple, indigo, brown, and other colors.

[0056] In one embodiment, suitable preservatives include, but are not limited to, at least one of p-hydroxyacetophenone, caprylyl glycol, chlorphenesin, methylparaben, potassium sorbate, sodium benzoate, phenoxyethanol, capryloyl hydroxamic acid, glyceryl caprylate, cetylpyridinium chloride, and cetylpyridinium chloride.

[0057] An embodiment of this application also provides a method for preparing a mild whitening and spot-fading lotion, comprising the following steps: (1) Add thickener, chelating agent, glyceryl glucoside and humectant to water and stir evenly at 70~90℃ to obtain aqueous phase; (2) Isopropyl myristate, jojoba seed oil, squalane, and emulsifier are stirred evenly at 60~90℃ to obtain the oil phase; (3) Add the oil phase to the aqueous phase, homogenize, stir evenly to obtain an emulsion, cool to 25~45℃, add glycyrrhizin, centella asiatica extract and whitening and spot-fading composition, stir evenly to obtain a mild whitening and spot-fading emulsion.

[0058] The present application is further illustrated below with specific embodiments:

[0059] Example 1 A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.04% glycyrrhizin, 0.05% disodium EDTA, 0.16% xanthan gum, 0.5% glyceryl glucoside, 0.6% Centella asiatica extract, 1.8% isopropyl myristate, 2% jojoba seed oil, 2.4% whitening and spot-fading composition, 3% squalane, 4.2% emulsifier, 8% 1,4-butanediol, and the balance being water.

[0060] The whitening and spot-fading composition comprises Bifida ferment filtrate, Albizia julibrissin bark extract, and Cherry blossom extract in a mass ratio of 40:30:30.

[0061] The emulsifier comprises polyglycerol-10 oleate and cetearyl alcohol in a mass ratio of 1:0.5.

[0062] The preparation method of the Albizia julibrissin bark extract is as follows: (1) Wash the bark of the Albizia julibrissin tree, dry it to constant weight, and grind it to 200 mesh to obtain Albizia julibrissin bark powder; Albizia bark powder, cellulase, β-glucosidase, and xylanase were added to water and enzymatically hydrolyzed at 48°C for 6 hours using ultrasound at 400W to obtain the enzymatic hydrolysate. The mass ratio of Albizia bark powder, cellulase, β-glucosidase, xylanase, and water was 1:0.01:0.005:0.005:10.

[0063] (2) The enzyme hydrolysate is first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5KD, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 0.5KD to obtain the filtrate; (3) Pass the filtrate through HDP-600 macroporous adsorption resin at a flow rate of 1 BV / h. The elution rate is 1 BV / h. First wash with water for 1.5 BV, then wash with 70 wt% ethanol solution for 1.5 BV. Collect the ethanol eluent, dry it, and obtain Albizia bark extract.

[0064] The preparation method of the mild whitening and spot-fading lotion includes the following steps: (1) Add xanthan gum, disodium EDTA, glyceryl glucoside and 1,4-butanediol to water and stir at 100 rpm for 0.5 h at 80 °C to obtain an aqueous phase; (2) Isopropyl myristate, jojoba seed oil, squalane and emulsifier were stirred at 85°C and 100 rpm for 15 min to obtain the oil phase; (3) Add the oil phase to the aqueous phase, homogenize at 1500 rpm for 6 min, and then stir at 100 rpm for 0.5 h to obtain an emulsion. Cool down to 40℃, add glycyrrhizin, Centella asiatica extract and whitening and spot-fading composition, and stir at 100 rpm for 0.5 h to obtain a mild whitening and spot-fading emulsion.

[0065] Example 2 A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.01% glycyrrhizin, 0.02% disodium EDTA, 0.1% xanthan gum, 0.6% glyceryl glucoside, 1% Centella asiatica extract, 1% isopropyl myristate, 3% jojoba seed oil, 2% whitening and spot-fading composition, 4% squalane, 5% emulsifier, 6% 1,4-butanediol, and the balance being water.

[0066] The whitening and spot-fading composition comprises Bifida ferment filtrate, Albizia julibrissin bark extract, and Cherry blossom extract in a mass ratio of 50:25:25.

[0067] The emulsifier comprises polyglycerol-10 oleate and cetearyl alcohol in a mass ratio of 1:0.5.

[0068] The preparation method of the Albizia julibrissin bark extract is as follows: (1) Wash the bark of the Albizia julibrissin tree, dry it to constant weight, and grind it to 200 mesh to obtain Albizia julibrissin bark powder; Albizia bark powder, cellulase, β-glucosidase, and xylanase were added to water and enzymatically hydrolyzed at 48°C for 6 hours using 400W ultrasound to obtain the enzymatic hydrolysate. The mass ratio of Albizia bark powder, cellulase, β-glucosidase, xylanase, and water was 1:0.012:0.004:0.004:10.

[0069] (2) The enzyme hydrolysate is first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5KD, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 0.5KD to obtain the filtrate; (3) Pass the filtrate through HDP-600 macroporous adsorption resin at a flow rate of 1 BV / h. The elution rate is 1 BV / h. First wash with water for 1.5 BV, then wash with 70 wt% ethanol solution for 1.5 BV. Collect the ethanol eluent, dry it, and obtain Albizia bark extract.

[0070] The preparation method of the mild whitening and spot-fading lotion includes the following steps: (1) Add xanthan gum, disodium EDTA, glyceryl glucoside and 1,4-butanediol to water and stir at 100 rpm for 0.5 h at 80 °C to obtain an aqueous phase; (2) Isopropyl myristate, jojoba seed oil, squalane and emulsifier were stirred at 85°C and 100 rpm for 15 min to obtain the oil phase; (3) Add the oil phase to the aqueous phase, homogenize at 1500 rpm for 6 min, and then stir at 100 rpm for 0.5 h to obtain an emulsion. Cool down to 40℃, add glycyrrhizin, Centella asiatica extract and whitening and spot-fading composition, and stir at 100 rpm for 0.5 h to obtain a mild whitening and spot-fading emulsion.

[0071] Example 3 A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.05% glycyrrhizin, 0.1% disodium EDTA, 0.2% xanthan gum, 0.2% glyceryl glucoside, 0.2% Centella asiatica extract, 2.5% isopropyl myristate, 1% jojoba seed oil, 3% whitening and spot-fading composition, 1% squalane, 3% emulsifier, 10% 1,4-butanediol, and the balance being water.

[0072] The whitening and spot-fading composition comprises Bifida ferment filtrate, Albizia julibrissin bark extract, and Cherry blossom extract in a mass ratio of 25:40:35.

[0073] The emulsifier comprises polyglycerol-10 oleate and cetearyl alcohol in a mass ratio of 1:0.5.

[0074] The preparation method of the Albizia julibrissin bark extract is as follows: (1) Wash the bark of the Albizia julibrissin tree, dry it to constant weight, and grind it to 200 mesh to obtain Albizia julibrissin bark powder; Albizia bark powder, cellulase, β-glucosidase, and xylanase were added to water and enzymatically hydrolyzed at 400W ultrasound and 48℃ for 6 hours to obtain an enzymatic hydrolysate. The mass ratio of Albizia bark powder, cellulase, β-glucosidase, xylanase, and water was 1:0.006:0.008:0.008:10.

[0075] (2) The enzyme hydrolysate is first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5KD, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 0.5KD to obtain the filtrate; (3) Pass the filtrate through HDP-600 macroporous adsorption resin at a flow rate of 1 BV / h. The elution rate is 1 BV / h. First wash with water for 1.5 BV, then wash with 70 wt% ethanol solution for 1.5 BV. Collect the ethanol eluent, dry it, and obtain Albizia bark extract.

[0076] The preparation method of the mild whitening and spot-fading lotion includes the following steps: (1) Add xanthan gum, disodium EDTA, glyceryl glucoside and 1,4-butanediol to water and stir at 100 rpm for 0.5 h at 80 °C to obtain an aqueous phase; (2) Isopropyl myristate, jojoba seed oil, squalane and emulsifier were stirred at 85°C and 100 rpm for 15 min to obtain the oil phase; (3) Add the oil phase to the aqueous phase, homogenize at 1500 rpm for 6 min, and then stir at 100 rpm for 0.5 h to obtain an emulsion. Cool down to 40℃, add glycyrrhizin, Centella asiatica extract and whitening and spot-fading composition, and stir at 100 rpm for 0.5 h to obtain a mild whitening and spot-fading emulsion.

[0077] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that the mass ratios of Bifida ferment filtrate, Albizia julibrissin bark extract, and Cherry blossom extract are different, while all other ratios are the same.

[0078] A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.04% glycyrrhizin, 0.05% disodium EDTA, 0.16% xanthan gum, 0.5% glyceryl glucoside, 0.6% Centella asiatica extract, 1.8% isopropyl myristate, 2% jojoba seed oil, 2.4% whitening and spot-fading composition, 3% squalane, 4.2% emulsifier, 8% 1,4-butanediol, and the balance being water.

[0079] The whitening and spot-fading composition comprises Bifida ferment filtrate, Albizia julibrissin bark extract, and Cherry blossom extract in a mass ratio of 70:15:15.

[0080] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that the mass ratios of Bifida ferment filtrate, Albizia julibrissin bark extract, and Cherry blossom extract are different, while all other ratios are the same.

[0081] A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.04% glycyrrhizin, 0.05% disodium EDTA, 0.16% xanthan gum, 0.5% glyceryl glucoside, 0.6% Centella asiatica extract, 1.8% isopropyl myristate, 2% jojoba seed oil, 2.4% whitening and spot-fading composition, 3% squalane, 4.2% emulsifier, 8% 1,4-butanediol, and the balance being water.

[0082] The whitening and spot-fading composition comprises Bifida ferment filtrate, Albizia julibrissin bark extract, and Cherry blossom extract in a mass ratio of 10:45:45.

[0083] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain Bifida Ferment Lysate Ferment Filtrate, the total amount of the whitening and spot-fading composition remains unchanged, and everything else is the same.

[0084] A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.04% glycyrrhizin, 0.05% disodium EDTA, 0.16% xanthan gum, 0.5% glyceryl glucoside, 0.6% Centella asiatica extract, 1.8% isopropyl myristate, 2% jojoba seed oil, 2.4% whitening and spot-fading composition, 3% squalane, 4.2% emulsifier, 8% 1,4-butanediol, and the balance being water.

[0085] The whitening and spot-fading composition comprises Albizia julibrissin bark extract and Cherry blossom extract in a mass ratio of 30:30.

[0086] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not contain Albizia bark extract, the total amount of the whitening and spot-fading composition remains the same, and everything else is the same.

[0087] A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.04% glycyrrhizin, 0.05% disodium EDTA, 0.16% xanthan gum, 0.5% glyceryl glucoside, 0.6% Centella asiatica extract, 1.8% isopropyl myristate, 2% jojoba seed oil, 2.4% whitening and spot-fading composition, 3% squalane, 4.2% emulsifier, 8% 1,4-butanediol, and the balance being water.

[0088] The whitening and spot-fading composition comprises Bifida ferment filtrate and Cherry blossom extract in a mass ratio of 40:30.

[0089] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not contain fine-toothed cherry blossom extract, the total amount of the whitening and spot-fading composition remains the same, and everything else is the same.

[0090] A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.04% glycyrrhizin, 0.05% disodium EDTA, 0.16% xanthan gum, 0.5% glyceryl glucoside, 0.6% Centella asiatica extract, 1.8% isopropyl myristate, 2% jojoba seed oil, 2.4% whitening and spot-fading composition, 3% squalane, 4.2% emulsifier, 8% 1,4-butanediol, and the balance being water.

[0091] The whitening and spot-fading composition includes Bifida ferment filtrate and Albizia julibrissin bark extract in a mass ratio of 40:30.

[0092] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that the whitening and spot-fading composition of Comparative Example 6 is different from that of Example 1, but everything else is the same.

[0093] A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.04% glycyrrhizin, 0.05% disodium EDTA, 0.16% xanthan gum, 0.5% glyceryl glucoside, 0.6% Centella asiatica extract, 1.8% isopropyl myristate, 2% jojoba seed oil, 2.4% whitening and spot-fading composition, 3% squalane, 4.2% emulsifier, 8% 1,4-butanediol, and the balance being water.

[0094] The whitening and spot-fading composition comprises Bifida ferment filtrate, nicotinamide, and licorice extract in a mass ratio of 40:30:30.

[0095] Comparative Example 7 The difference between Comparative Example 7 and Example 1 is that the whitening and spot-fading composition of Comparative Example 7 is different from that of Example 1, but everything else is the same.

[0096] A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.04% glycyrrhizin, 0.05% disodium EDTA, 0.16% xanthan gum, 0.5% glyceryl glucoside, 0.6% Centella asiatica extract, 1.8% isopropyl myristate, 2% jojoba seed oil, 2.4% whitening and spot-fading composition, 3% squalane, 4.2% emulsifier, 8% 1,4-butanediol, and the balance being water.

[0097] The whitening and spot-fading composition comprises niacinamide, Albizia julibrissin bark extract, and Rhodiola rosea root extract in a mass ratio of 40:30:30.

[0098] Comparative Example 8 The difference between Comparative Example 8 and Example 1 is that the whitening and spot-fading composition of Comparative Example 8 is different from that of Example 1, but everything else is the same.

[0099] A mild whitening and spot-fading lotion comprising the following components by weight percentage: 0.04% glycyrrhizin, 0.05% disodium EDTA, 0.16% xanthan gum, 0.5% glyceryl glucoside, 0.6% Centella asiatica extract, 1.8% isopropyl myristate, 2% jojoba seed oil, 2.4% whitening and spot-fading composition, 3% squalane, 4.2% emulsifier, 8% 1,4-butanediol, and the balance being water.

[0100] The whitening and spot-fading composition comprises licorice extract, rhodiola rosea root extract, and cherry blossom extract in a mass ratio of 40:30:30.

[0101] Comparative Example 9 The difference between Comparative Example 9 and Example 1 is that the preparation method of the Albizia julibrissin bark extract is different, but everything else is the same.

[0102] The preparation method of the Albizia julibrissin bark extract is as follows: (1) Wash the bark of the Albizia julibrissin tree, dry it to constant weight, and grind it to 200 mesh to obtain Albizia julibrissin bark powder; Albizia bark powder, cellulase, pectinase, and amylase were added to water and enzymatically hydrolyzed at 48°C for 6 hours using 400W ultrasound to obtain the enzymatic hydrolysate. The mass ratio of Albizia bark powder, cellulase, pectinase, amylase, and water was 1:0.01:0.005:0.005:10.

[0103] (2) The enzyme hydrolysate is first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5KD, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 0.5KD to obtain the filtrate; (3) Pass the filtrate through HDP-600 macroporous adsorption resin at a flow rate of 1 BV / h. The elution rate is 1 BV / h. First wash with water for 1.5 BV, then wash with 70 wt% ethanol solution for 1.5 BV. Collect the ethanol eluent, dry it, and obtain Albizia bark extract.

[0104] Comparative Example 10 The difference between Comparative Example 10 and Example 1 is that the preparation method of the Albizia julibrissin bark extract is different, but everything else is the same.

[0105] The preparation method of the Albizia julibrissin bark extract is as follows: (1) Wash the bark of the Albizia julibrissin tree, dry it to constant weight, and grind it to 200 mesh to obtain Albizia julibrissin bark powder; Albizia bark powder, cellulase, β-glucosidase, and xylanase were added to water and enzymatically hydrolyzed at 48°C for 6 hours using ultrasound at 400W to obtain the enzymatic hydrolysate. The mass ratio of Albizia bark powder, cellulase, β-glucosidase, xylanase, and water was 1:0.01:0.005:0.005:10.

[0106] (2) The enzymatic hydrolysate was first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 1KD, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5KD. After drying, the extract of Albizia julibrissin bark was obtained.

[0107] Test case 1. Whitening and Spot-Fading Test: 1.1 Volunteer selection: 130 volunteers were selected and randomly divided into 13 groups of 10 each. Volunteers in each group tried the emulsion described in the examples and comparative examples.

[0108] 1.1.1 The inclusion criteria for volunteers are: female aged 18-35 years; no use of hormone drugs or immunosuppressants in the past month; no participation in other clinical trials at the test site in the present or in the past 30 days; able to cooperate well with the trial; able to read and understand all the contents of the informed consent form and voluntarily sign the informed consent form; and agree not to use any cosmetics, drugs and health products that may affect the results during the trial.

[0109] 1.1.2 Exclusion Criteria: The skin at the test site must be in normal condition, without open wounds, erosion, severe acne, eczema, psoriasis, seborrheic dermatitis, or other skin diseases that might affect the evaluation. The candidate must not have participated in a similar human efficacy evaluation trial for cosmetics within the past 3 months, or any previous darkening marks on the test site must have completely faded. The candidate must voluntarily participate in this trial, understand the purpose and procedure of the trial, sign a written informed consent form, and promise to comply with the trial requirements, follow up on time, and truthfully report their experience and adverse reactions.

[0110] 1.1.3 Criteria for termination and withdrawal: failure to apply test samples as required or failure to record follow-up information as required during the test; serious illness or serious adverse reaction during use.

[0111] 1.1.4 Data Processing for Dropout Subjects: For any dropout subject, the investigator must accurately record the reason and time of dropout in the Clinical Trial Record Form (CRF). Reasons for dropout include: adverse event, lack of efficacy, protocol violation (poor compliance), loss to follow-up (voluntary withdrawal), sponsor withdrawal, and other.

[0112] 1.2 Test method: Volunteers used the above products on their entire face after cleansing in the morning and evening for 28 consecutive days.

[0113] 1.2.1 On day 0 and day 28 of the trial samples, the melanin index (MI value) of the same area of ​​the volunteers was measured using a skin red melanin analyzer from Courage+Khazaka (Germany). Three tests were performed for each sample, and the average value was calculated. The melanin change rate was calculated as 100%. [(MI 28- MI0) / (MI0)).

[0114] 1.2.2 On day 0 and day 28 of the trial samples, the skin pigmentation area (PS) of the same area of ​​the volunteers was tested using the VISIA skin testing system. The rate of change in pigmentation area was 100%. PS 28 -PS0) / (PS0)].

[0115] Table 1

[0116] As can be seen from Table 1, the emulsion described in this application has excellent whitening and spot-fading effects.

[0117] Comparing Example 1 with Comparative Examples 1-2, it can be seen that by controlling the mass ratio of Bifida ferment filtrate, Albizia julibrissin bark extract and Cherry blossom extract to (25-50): (25-40): (25-35), this application significantly improves the whitening and spot-fading effect.

[0118] Comparing Example 1 with Comparative Examples 3-8, it can be seen that the Bifida ferment filtrate, Albizia julibrissin bark extract, and Cherry blossom extract have a significant synergistic effect in whitening and fading spots. Under the synergistic effect of the three, the whitening and spot-fading effect is significantly improved.

[0119] Comparing Example 1 with Comparative Examples 9-10, it can be seen that the Albizia bark extract prepared in this application can significantly improve the whitening and spot-fading effects.

[0120] 2. Stability Test: The samples prepared in the examples and comparative examples were used as test samples. Eight 25g samples were weighed into each transparent PET bottle and placed under constant temperature conditions of -18℃, 25℃ and 50℃ for 60 days. After returning to room temperature, their stability was observed. The criteria for judging stability were that the sample appearance did not show any layering or oil seepage after returning to room temperature, and the transparency did not change. Otherwise, it was considered abnormal.

[0121] Table 2

[0122] As can be seen from Table 2, the emulsion described in this application has good stability.

[0123] 3. Stimulation test: The emulsion was tested according to the method of SNT 2329-2009, and the results were evaluated using the stimulation rating method.

[0124] The specific scores are shown in Table 3, and the evaluation results are shown in Table 4.

[0125] Table 3

[0126] Table 4

[0127] As can be seen from Table 4, the emulsion described in this application has good safety, is mild and non-irritating, and will not cause irritation or allergic reactions.

[0128] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit the scope of protection of this application. Although this application has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the substance and scope of the technical solutions of this application.

Claims

1. A mild whitening and spot-fading lotion, characterized in that, The product comprises the following components by weight percentage: 0.01-0.05% glycyrrhizin, 0.02-0.1% chelating agent, 0.1-0.2% thickener, 0.2-0.6% glyceryl glucoside, 0.2-1% Centella asiatica extract, 1-2.5% isopropyl myristate, 1-3% jojoba seed oil, 2-3% whitening and spot-fading composition, 1-4% squalane, 3-5% emulsifier, 6-10% moisturizer, and the balance being water; The whitening and spot-fading composition comprises Bifida ferment filtrate, Albizia julibrissin bark extract, and Cherry blossom extract in a mass ratio of (25~50):(25~40):(25~35).

2. The mild whitening and spot-fading lotion according to claim 1, characterized in that, The preparation method of the Albizia julibrissin bark extract is as follows: (1) Add Albizia bark powder, cellulase, β-glucosidase and xylanase to water, and sonicate to hydrolyze to obtain hydrolysate; (2) The enzyme hydrolysate is first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5KD, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 0.5KD to obtain the filtrate; (3) The filtrate was separated by passing it through a macroporous adsorption resin and dried to obtain an extract of Albizia julibrissin bark.

3. The mild whitening and spot-fading lotion according to claim 2, characterized in that, The mass ratio of the Albizia julibrissin bark powder, cellulase, β-glucosidase, xylanase, and water is 1:(0.006~0.012):(0.004~0.008):(0.004~0.008):(8~20).

4. The mild whitening and spot-fading lotion according to claim 2, characterized in that, The separation of the filtrate by passing it through a macroporous adsorption resin is specifically as follows: the filtrate is passed through the macroporous adsorption resin at a flow rate of 0.8~1.5 BV / h, the elution rate is 0.8~1.5 BV / h, it is first washed with water for 1~2 BV, and then washed with 50~75wt% ethanol solution for 0.8~2 BV, and the ethanol eluent is collected.

5. The mild whitening and spot-fading lotion according to claim 2, characterized in that, The ultrasonic enzymatic hydrolysis is performed at a temperature of 42-50℃ for 2-10 hours, with an ultrasonic power of 200-600W.

6. The mild whitening and spot-fading lotion according to claim 2, characterized in that, The macroporous adsorption resin is at least one of HDP-600 macroporous resin and HDP-500 macroporous resin.

7. The mild whitening and spot-fading lotion according to claim 1, characterized in that, The moisturizer includes at least one of 1,4-butanediol, 1,3-propanediol, 1,2-hexanediol, dipropylene glycol, and glycerin. The thickener includes at least one of carbomer, xanthan gum, argan gum, hydroxyethyl cellulose, ammonium acryloyl dimethyl taurate / VP copolymer, and polyacrylate crosspolymer-6.

8. The mild whitening and spot-fading lotion according to claim 1, characterized in that, The chelating agent is disodium EDTA.

9. The mild whitening and spot-fading lotion according to claim 1, characterized in that, The emulsifier comprises polyglycerol-10 oleate and cetearyl alcohol in a mass ratio of 1:(0.4~0.8).

10. The method for preparing the mild whitening and spot-fading emulsion according to any one of claims 1 to 9, characterized in that, Includes the following steps: (1) Add thickener, chelating agent, glyceryl glucoside and humectant to water and stir evenly at 70~90℃ to obtain aqueous phase; (2) Isopropyl myristate, jojoba seed oil, squalane, and emulsifier are stirred evenly at 60~90℃ to obtain the oil phase; (3) Add the oil phase to the aqueous phase, homogenize, stir evenly to obtain an emulsion, cool to 25~45℃, add glycyrrhizin, centella asiatica extract and whitening and spot-fading composition, stir evenly to obtain a mild whitening and spot-fading emulsion.

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