A homologous composition for improving sleep and invigorating spleen and blood and a preparation method thereof
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- 罗占光
- Filing Date
- 2026-05-12
- Publication Date
- 2026-07-14
AI Technical Summary
Existing compositions for improving sleep have low bioavailability of active ingredients, which limits their practical effectiveness.
It uses a combination of medicinal and edible ingredients such as Codonopsis pilosula, Poria cocos, Cistanche deserticola, Angelica sinensis, lotus seeds, and Ziziphus jujuba var. spinosa. Through honey-processing and fermentation with compound probiotics, the tissue structure of Ziziphus jujuba var. spinosa is improved. The enzyme system complementarity of Lactobacillus plantarum and Saccharomyces cerevisiae is utilized to decompose large molecular active ingredients into small molecules, thereby improving the dissolution and absorption of active ingredients.
It improves the bioavailability of active ingredients, achieves efficient dissolution and absorption of active ingredients, and enhances the effects of the composition on improving sleep and nourishing the spleen and blood.
Smart Images
Figure CN122376648A_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of health-preserving composition technology, and more specifically, it relates to a homologous composition for improving sleep and strengthening the spleen and nourishing blood, and a method for preparing the same. Background Technology
[0002] Food-medicine homology refers to traditional Chinese medicinal materials that possess both edible and medicinal value. These materials are highly safe, have few side effects, and are suitable for long-term conditioning. With increasing health awareness, food-medicine homology products have received widespread attention for improving sub-health conditions and regulating physiological functions. Sleep disorders are a common manifestation of sub-health, which traditional Chinese medicine believes is often related to deficiency of both the heart and spleen, and insufficient qi and blood. Conditioning with food-medicine homology formulas that strengthen the spleen, nourish blood, and calm the mind is a safe and effective intervention method.
[0003] Currently, compositions used to improve sleep typically use raw materials such as Coptis chinensis, Scutellaria baicalensis, Paeonia lactiflora, donkey-hide gelatin, and egg yolk, which are decocted in water and then concentrated into capsules. However, the large molecular active ingredients in these medicinal materials, such as polysaccharides and glycosides, are difficult to fully dissolve, which can lead to low bioavailability of the active ingredients. Summary of the Invention
[0004] To address the problem that existing compositions for improving sleep often result in low bioavailability of active ingredients, this application provides a composition that improves sleep and nourishes the spleen and blood, and its preparation method.
[0005] This application provides a homologous composition for improving sleep and strengthening the spleen and nourishing blood, and its preparation method, which adopts the following technical solution:
[0006] In a first aspect, this application provides a composition that improves sleep and strengthens the spleen and nourishes blood, employing the following technical solution:
[0007] A composition for improving sleep and strengthening the spleen and nourishing blood, comprising the following medicinal and edible ingredients in parts by weight: Codonopsis pilosula 8-16 parts, Poria cocos 12-24 parts, Cistanche deserticola 4-8 parts, Angelica sinensis 1-3 parts, Nelumbo nucifera 5-10 parts, and Ziziphus jujuba var. spinosa 4-8 parts.
[0008] By adopting the above technical solution, and using six medicinal and edible raw materials—Codonopsis pilosula, Poria cocos, Cistanche deserticola, Angelica sinensis, Nelumbo nucifera, and Ziziphus jujuba var. spinosa—Codonopsis pilosula, Poria cocos, and Nelumbo nucifera synergistically strengthen the spleen and replenish qi to nourish the source of qi and blood; Angelica sinensis nourishes and harmonizes blood; Cistanche deserticola warms and tonifies the kidneys to assist yang and generate yin; and Ziziphus jujuba var. spinosa nourishes the heart and calms the mind. The combined effects of these herbs strengthen the spleen, nourish blood, and calm the mind. At the same time, the combination of raw materials has good water solubility and compatibility, and the active ingredients are not prone to antagonistic reactions, which is conducive to the dissolution and absorption of effective ingredients in the body. Therefore, the bioavailability of active ingredients is improved, solving the problem that existing compositions for improving sleep often result in low bioavailability of active ingredients.
[0009] Preferably, the jujube seed is honey-processed jujube seed.
[0010] By adopting the above technical solution, the jujube seed is processed by honey roasting. The honey roasting process can change the physicochemical properties of the jujube seed, making its surface have a microporous structure, which is conducive to the penetration of water and the attachment of microorganisms during the subsequent fermentation process. At the same time, the caramelization products produced by honey roasting can provide additional carbon source for probiotics.
[0011] Secondly, this application provides a method for preparing a homologous composition for improving sleep and strengthening the spleen and nourishing blood, using the following technical solution:
[0012] A method for preparing a sleep-improving and spleen-strengthening and blood-nourishing homologous composition, applied to the above-mentioned sleep-improving and spleen-strengthening and blood-nourishing homologous composition, includes the following steps:
[0013] Step 1: According to the weight proportions, honey-process the jujube seeds, and dry and grind the codonopsis, poria, cistanche, angelica and lotus seeds separately to obtain the processed raw materials;
[0014] Step 2: Mix the raw materials thoroughly to obtain a mixed powder;
[0015] Step 3: The mixed powder is fermented with compound probiotics to obtain fermentation products;
[0016] Step 4: Formulate the fermentation product into an oral dosage form to complete the preparation.
[0017] By adopting the above technical solution, the jujube seeds are pretreated by honey-processing, and then mixed with other raw materials for compound probiotic fermentation. Honey-processing improves the tissue structure of the jujube seeds, creating favorable conditions for subsequent fermentation. Compound probiotic fermentation can decompose the large-molecule polysaccharides, glycosides and other substances in the raw materials into small-molecule active ingredients, while the probiotics metabolize to produce neuroactive substances such as GABA.
[0018] Preferably, in step one, the jujube seed undergoes honey-processing, which includes the following steps:
[0019] Take jujube seeds, add refined honey water and mix well. Let it soak until the refined honey water is absorbed. Place it in a hot pan and stir-fry over low heat at 140-160℃ for 3-6 minutes, until the surface is slightly yellow and not sticky. The volume ratio of refined honey to water in the refined honey water is 1:0.3-1:0.5, and the weight ratio of jujube seeds to refined honey is 100:5-10.
[0020] By adopting the above technical solution, the proportion of refined honey, the frying temperature and time in the honey-roasting process can ensure that the jujube seeds are heated evenly and fully absorb the refined honey, achieving the processing standard of a slightly yellow surface and non-sticky texture. This avoids insufficient honey-roasting effect due to under-roasting and also prevents damage to active ingredients due to over-roasting.
[0021] Preferably, in step one, the drying temperature is 40-60°C, and the drying is carried out until the moisture content is less than 10%, and the particle size of the pulverized material is sieved through a 40-80 mesh sieve.
[0022] By adopting the above technical solution, the drying temperature, moisture content, and particle size can be fully dried to a suitable degree of pulverization, and the particle size after pulverization can ensure the uniformity of subsequent mixing and the effective contact of microorganisms during fermentation.
[0023] Preferably, in step two, the mixing is carried out using a three-dimensional motion mixer with a mixer speed of 20-40 rpm and a mixing time of 30-60 minutes.
[0024] By adopting the above technical solution and using a three-dimensional motion mixer for mixing, the powder of the six ingredients can move fully and be evenly distributed in three-dimensional space, avoiding batch-to-batch differences caused by uneven mixing.
[0025] Preferably, in step three, the fermentation and transformation of the compound probiotics includes the following steps:
[0026] The mixed powder is placed in a fermenter, and sterile water is added at a ratio of 3 to 5 times the total mass of the mixed powder. A compound probiotic agent is inoculated, and the fermentation temperature is controlled at 30 to 37°C. The mixture is allowed to ferment under anaerobic conditions for 24 to 72 hours. The compound probiotic agent is composed of Lactobacillus plantarum and Saccharomyces cerevisiae at a mass ratio of 1.5:1 to 2.5:1, and the inoculation amount is 1% to 3% of the total mass of the mixed powder.
[0027] After fermentation, the fermentation broth is heated to 80-85℃ and kept at that temperature for 25-35 minutes for inactivation treatment to obtain the fermentation product.
[0028] By adopting the above technical solutions, the fermentation water volume, strain composition and ratio, inoculum amount, temperature, time and inactivation conditions enable Lactobacillus plantarum and Saccharomyces cerevisiae to produce synergy. Saccharomyces cerevisiae uses oxygen to consume and produce ethanol and carbon dioxide, creating an anaerobic environment for Lactobacillus plantarum. At the same time, the enzyme systems produced by the metabolism of the two complement each other, promoting polysaccharide degradation and GABA enrichment.
[0029] Preferably, after obtaining the fermentation product, it is spray-dried to produce fermentation powder or centrifuged and filtered to produce fermentation liquid. The inlet air temperature of the spray drying is 160-200℃ and the outlet air temperature is 80-100℃. The centrifugal speed of the centrifugation filtration is 3000-5000 rpm and the filter pore size is 0.45-1.0 micrometers.
[0030] By adopting the above technical solutions, the fermentation products can be spray-dried into fermentation powder or centrifuged and filtered into fermentation broth as needed. Spray drying can quickly remove moisture and maintain the stability of active ingredients, while centrifugation and filtration can obtain a clear fermentation broth that is easy to use directly.
[0031] Preferably, in step four, the oral dosage form includes powder, pill, capsule, tablet, oral liquid or granule.
[0032] By adopting the above technical solutions, fermentation products can be made into various oral dosage forms such as powders, pills, capsules, tablets, oral liquids or granules. Different dosage forms can adapt to the different people's taking habits and absorption characteristics, thus expanding the applicable scenarios of the products.
[0033] Preferably, when the oral dosage form is a capsule, the baking powder and magnesium stearate are mixed evenly, filled into gelatin capsules, each capsule containing 0.3-0.5g, and the amount of magnesium stearate mixed is 0.5%-1.0% of the mass of the baking powder;
[0034] When the oral dosage form is an oral liquid, the fermentation broth is diluted with purified water at a volume ratio of 1:1 to 1:3, and potassium sorbate at a final concentration of 0.05% to 0.1% is added as a preservative. The mixture is then filled into glass bottles, with each bottle containing 10 to 20 mL.
[0035] By adopting the above technical solutions, adding magnesium stearate as a lubricant when preparing capsules can ensure smooth capsule filling and uniform filling volume; diluting and adding potassium sorbate as a preservative when preparing oral liquids can maintain the microbial stability of the product during storage.
[0036] In summary, this application has the following beneficial effects:
[0037] 1. This application uses six medicinal and edible ingredients—Codonopsis pilosula, Poria cocos, Cistanche deserticola, Angelica sinensis, Nelumbo nucifera, and Ziziphus jujuba var. spinosa—to create a formula. Codonopsis pilosula, Poria cocos, and Nelumbo nucifera work together to strengthen the spleen and replenish qi, thus nourishing the source of qi and blood. Angelica sinensis nourishes and harmonizes the blood. Cistanche deserticola warms and tonifies the kidneys to assist yang and generate yin. Ziziphus jujuba var. spinosa nourishes the heart and calms the mind. The combined effects of these herbs strengthen the spleen, nourish the blood, and calm the mind. At the same time, the combination of ingredients has good water solubility and compatibility, and the active ingredients are less likely to antagonize each other, which is conducive to the dissolution and absorption of the active ingredients in the body. Therefore, it achieves the effect of improving the bioavailability of active ingredients and solves the problem that existing compositions for improving sleep tend to have low bioavailability of active ingredients.
[0038] 2. In this application, the jujube seed is processed by honey-roasting and combined with fermentation and transformation by a compound probiotic of Lactobacillus plantarum and Saccharomyces cerevisiae. The honey-roasting process improves the tissue structure of the jujube seed and introduces micropores on its surface, which is conducive to the attachment and metabolism of microorganisms during the subsequent fermentation process. The compound probiotic fermentation and transformation decomposes the macromolecular polysaccharides into small molecule active ingredients and enriches GABA through enzyme complementarity between strains.
[0039] 3. This application ensures the stability and reproducibility of the preparation method by controlling the proportion of refined honey, the range of roasting temperature and time during the honey-roasting process, and the amount of water, inoculation amount, temperature, and time during the fermentation process. At the same time, the fermentation product can be made into various dosage forms such as capsules or oral liquids as needed to suit the consumption habits of different groups of people. Attached Figure Description
[0040] Figure 1 This is a flowchart of a method for preparing a homologous composition for improving sleep and strengthening the spleen and nourishing blood, as provided in this application. Detailed Implementation
[0041] The present application will be further described in detail below with reference to the accompanying drawings and embodiments.
[0042] Technical concept: The macromolecular active ingredients such as polysaccharides and glycosides in existing sleep-improving compositions are difficult to fully dissolve under conventional water decoction or direct pulverization processes, resulting in low bioavailability of active ingredients and limiting the practical application effect of sleep-improving compositions.
[0043] Based on this, this application describes a method where honey-processing of jujube seeds creates a microporous structure on their surface and alters their physicochemical properties, facilitating water penetration and microbial adhesion during subsequent fermentation. Simultaneously, a compound probiotic agent composed of *Lactobacillus plantarum* and *Saccharomyces cerevisiae* is used to ferment and transform the mixed powder. The two strains produce complementary enzyme systems during metabolism. *Saccharomyces cerevisiae* consumes oxygen to create an anaerobic environment for *Lactobacillus plantarum*, while *Lactobacillus plantarum* metabolizes to produce neuroactive substances such as γ-aminobutyric acid (GABA). This synergistic effect breaks down large polysaccharides and glycosides in the raw materials into smaller active ingredients, increasing the release rate of these active ingredients. Furthermore, the formulation ratio of the raw materials has been optimized, determining the weight proportions of *Codonopsis pilosula*, *Poria cocos*, *Cistanche deserticola*, *Angelica sinensis*, lotus seeds, and jujube seeds. This allows the six medicinal and edible ingredients to synergistically enhance their spleen-strengthening, blood-nourishing, heart-calming, and mind-soothing effects, achieving efficient release and transformation of active ingredients and improving their bioavailability. This solves the problem of existing sleep-improving compositions often resulting in low bioavailability of active ingredients.
[0044] Unless otherwise specified, all experimental methods used below are conventional methods. All materials, reagents, methods, and instruments used, unless otherwise specified, are conventional materials, reagents, methods, and instruments in this field, which can be obtained commercially or prepared according to literature methods by those skilled in the art.
[0045] To better understand the above technical solutions, the technical solutions of the present invention will be clearly and completely described below in conjunction with embodiments.
[0046] Please see the appendix Figure 1 The following is a further description with reference to the embodiments:
[0047] Example 1: A composition for improving sleep and strengthening the spleen and nourishing blood, comprising the following medicinal and edible ingredients in parts by weight: 12 parts Codonopsis pilosula, 18 parts Poria cocos, 5 parts Cistanche deserticola, 2 parts Angelica sinensis, 7.5 parts Nelumbo nucifera, and 6 parts Ziziphus jujuba var. spinosa.
[0048] Sour jujube seed is honey-fried sour jujube seed.
[0049] A method for preparing a sleep-improving and spleen-strengthening and blood-nourishing homologous composition, applied to the above-mentioned sleep-improving and spleen-strengthening and blood-nourishing homologous composition, includes the following steps:
[0050] Step 1: According to the weight proportions, honey-process the jujube seeds, and dry and grind the codonopsis, poria, cistanche, angelica and lotus seeds separately to obtain the processed raw materials;
[0051] The honey-processing of jujube seeds includes the following steps:
[0052] Take the jujube seeds, add them to the honey water and mix well. Let them soak until the honey water is absorbed. Place them in a hot pan and stir-fry over low heat at 150℃ for 4.5 minutes, until the surface is slightly yellow and not sticky. The volume ratio of honey to water in the honey water is 1:0.4, and the weight ratio of jujube seeds to honey is 100:7.5.
[0053] The drying temperature is 50℃, and the drying is carried out until the moisture content is less than 10%. The particle size of the pulverized material is sieved through a 60-mesh sieve.
[0054] Step 2: Mix the raw materials thoroughly to obtain a mixed powder;
[0055] The mixing process utilizes a three-dimensional motion mixer with a speed of 30 revolutions per minute and a mixing time of 45 minutes.
[0056] Step 3: The mixed powder is fermented with compound probiotics to obtain the fermentation product;
[0057] The fermentation and transformation of compound probiotics includes the following steps:
[0058] The mixed powder was put into a fermenter, and sterile water was added at a ratio of 4 times the total mass of the mixed powder. A compound probiotic agent was inoculated, and the fermentation temperature was controlled at 33.5℃. The mixture was allowed to ferment under anaerobic conditions for 48 hours. The compound probiotic agent was composed of Lactobacillus plantarum and Saccharomyces cerevisiae in a mass ratio of 2:1, and the inoculation amount was 2% of the total mass of the mixed powder.
[0059] After fermentation, the fermentation broth was heated to 82.5℃ and kept at that temperature for 30 minutes for inactivation treatment to obtain the fermentation product.
[0060] After obtaining the fermentation product, it is either spray-dried to make fermentation powder or centrifuged and filtered to make fermentation liquid. The inlet air temperature of spray drying is 180℃ and the outlet air temperature is 90℃. The centrifugal speed of centrifugation is 4000 rpm and the filter pore size is 0.73 micrometers.
[0061] Step 4: Formulate the fermentation product into an oral dosage form to complete the preparation.
[0062] Oral dosage forms include powders, pills, capsules, tablets, oral liquids, or granules.
[0063] When the oral dosage form is capsules, the baking powder and magnesium stearate are mixed evenly, filled into gelatin capsules, with each capsule containing 0.4g. The amount of magnesium stearate mixed is 0.75% of the mass of the baking powder.
[0064] When the oral dosage form is an oral liquid, the fermentation broth is diluted with purified water at a volume ratio of 1:2, and potassium sorbate at a final concentration of 0.075% is added as a preservative. The mixture is then filled into glass bottles, 15 mL per bottle.
[0065] Example 2: This example differs from Example 1 above in that:
[0066] A composition for improving sleep and strengthening the spleen and nourishing blood, comprising the following medicinal and edible ingredients in parts by weight: 8 parts Codonopsis pilosula, 12 parts Poria cocos, 4 parts Cistanche deserticola, 1 part Angelica sinensis, 5 parts Nelumbo nucifera, and 4 parts Ziziphus jujuba var. spinosa.
[0067] Sour jujube seed is honey-fried sour jujube seed.
[0068] A method for preparing a sleep-improving and spleen-strengthening and blood-nourishing homologous composition, applied to the above-mentioned sleep-improving and spleen-strengthening and blood-nourishing homologous composition, includes the following steps:
[0069] Step 1: According to the weight proportions, honey-process the jujube seeds, and dry and grind the codonopsis, poria, cistanche, angelica and lotus seeds separately to obtain the processed raw materials;
[0070] The honey-processing of jujube seeds includes the following steps:
[0071] Take the jujube seeds, add them to the honey water and mix well. Let them soak until the honey water is absorbed. Place them in a hot pan and stir-fry over a low heat of 140℃ for 3 minutes, until the surface is slightly yellow and no longer sticky. The volume ratio of honey to water in the honey water is 1:0.3, and the weight ratio of jujube seeds to honey is 100:5.
[0072] The drying temperature is 40℃, and the drying is carried out until the moisture content is less than 10%. The particle size of the pulverized material is sieved through a 40-mesh sieve.
[0073] Step 2: Mix the raw materials thoroughly to obtain a mixed powder;
[0074] The mixing process utilizes a three-dimensional motion mixer with a speed of 20 revolutions per minute and a mixing time of 30 minutes.
[0075] Step 3: The mixed powder is fermented with compound probiotics to obtain the fermentation product;
[0076] The fermentation and transformation of compound probiotics includes the following steps:
[0077] The mixed powder was put into a fermenter, and sterile water was added in a volume three times the total mass of the mixed powder. A compound probiotic agent was inoculated, and the fermentation temperature was controlled at 30°C. The mixture was allowed to ferment under anaerobic conditions for 24 hours. The compound probiotic agent consisted of Lactobacillus plantarum and Saccharomyces cerevisiae in a mass ratio of 1.5:1, and the inoculation amount was 1% of the total mass of the mixed powder.
[0078] After fermentation, the fermentation broth was heated to 80°C and kept at that temperature for 25 minutes to inactivate the fermentation product.
[0079] After obtaining the fermentation product, it is either spray-dried to make fermentation powder or centrifuged and filtered to make fermentation liquid. The inlet air temperature of spray drying is 160℃ and the outlet air temperature is 80℃. The centrifugal speed of centrifugation filtration is 3000 rpm and the filter pore size is 0.45 micrometers.
[0080] Step 4: Formulate the fermentation product into an oral dosage form to complete the preparation.
[0081] Oral dosage forms include powders, pills, capsules, tablets, oral liquids, or granules.
[0082] When the oral dosage form is capsules, the baking powder and magnesium stearate are mixed evenly, filled into gelatin capsules, with each capsule containing 0.3g of the mixture. The amount of magnesium stearate used is 0.5% of the mass of the baking powder.
[0083] When the oral dosage form is an oral liquid, the fermentation broth is diluted with purified water at a volume ratio of 1:1, and potassium sorbate at a final concentration of 0.05% is added as a preservative. The mixture is then filled into glass bottles, 10 mL per bottle.
[0084] Example 3: This example differs from Example 1 above in that:
[0085] A composition for improving sleep and strengthening the spleen and nourishing blood, comprising the following medicinal and edible ingredients in parts by weight: 16 parts Codonopsis pilosula, 24 parts Poria cocos, 8 parts Cistanche deserticola, 3 parts Angelica sinensis, 10 parts Nelumbo nucifera, and 8 parts Ziziphus jujuba var. spinosa.
[0086] Sour jujube seed is honey-fried sour jujube seed.
[0087] A method for preparing a sleep-improving and spleen-strengthening and blood-nourishing homologous composition, applied to the above-mentioned sleep-improving and spleen-strengthening and blood-nourishing homologous composition, includes the following steps:
[0088] Step 1: According to the weight proportions, honey-process the jujube seeds, and dry and grind the codonopsis, poria, cistanche, angelica and lotus seeds separately to obtain the processed raw materials;
[0089] The honey-processing of jujube seeds includes the following steps:
[0090] Take the jujube seeds, add them to the honey water and mix well. Let them soak until the honey water is absorbed. Place them in a hot pan and stir-fry over low heat at 160℃ for 6 minutes, until the surface is slightly yellow and no longer sticky. The volume ratio of honey to water in the honey water is 1:0.5, and the weight ratio of jujube seeds to honey is 100:10.
[0091] The drying temperature is 60℃, and the drying is carried out until the moisture content is less than 10%. The particle size of the pulverized material is sieved through an 80-mesh sieve.
[0092] Step 2: Mix the raw materials thoroughly to obtain a mixed powder;
[0093] The mixing process utilizes a three-dimensional motion mixer with a speed of 40 revolutions per minute and a mixing time of 60 minutes.
[0094] Step 3: The mixed powder is fermented with compound probiotics to obtain the fermentation product;
[0095] The fermentation and transformation of compound probiotics includes the following steps:
[0096] The mixed powder was put into a fermenter, and sterile water was added at a ratio of 5 times the total mass of the mixed powder. The compound probiotic agent was inoculated, and the fermentation temperature was controlled at 37°C. The mixture was allowed to ferment under anaerobic conditions for 72 hours. The compound probiotic agent was composed of Lactobacillus plantarum and Saccharomyces cerevisiae at a mass ratio of 2.5:1, and the inoculation amount was 3% of the total mass of the mixed powder.
[0097] After fermentation, the fermentation broth was heated to 85°C and kept at that temperature for 35 minutes to inactivate the fermentation product.
[0098] After obtaining the fermentation product, it is either spray-dried to make fermentation powder or centrifuged and filtered to make fermentation liquid. The inlet air temperature of spray drying is 200℃ and the outlet air temperature is 100℃. The centrifugal speed of centrifugation is 5000 rpm and the filter pore size is 1.0 micrometer.
[0099] Step 4: Formulate the fermentation product into an oral dosage form to complete the preparation.
[0100] Oral dosage forms include powders, pills, capsules, tablets, oral liquids, or granules.
[0101] When the oral dosage form is capsules, the baking powder and magnesium stearate are mixed evenly, filled into gelatin capsules, with each capsule containing 0.5g. The amount of magnesium stearate mixed is 1.0% of the mass of the baking powder.
[0102] When the oral dosage form is an oral liquid, the fermentation broth is diluted with purified water at a volume ratio of 1:3, and potassium sorbate at a final concentration of 0.1% is added as a preservative. The mixture is then filled into glass bottles, each containing 20 mL.
[0103] Comparative Example 1: A traditional Chinese medicine composition for improving sleep, comprising the following raw materials in parts by weight: 12 parts Coptis chinensis, 6 parts Scutellaria baicalensis, 6 parts Paeonia lactiflora, 9 parts donkey-hide gelatin, and 2 egg yolks.
[0104] The preparation method is as follows: remove impurities from Coptis chinensis, Scutellaria baicalensis and Paeonia lactiflora, wash them, and place them in a decoction pot. Add water equal to 8 times the total mass of the raw materials, soak for 30 minutes, heat to boiling, and then decoct for 30 minutes while maintaining a gentle boil. Filter and collect the decoction.
[0105] Add the dregs back to water, which is 6 times the total weight of the raw materials. Heat to a boil and then simmer for 20 minutes. Filter and collect the decoction.
[0106] Combine the two decoctions, add donkey-hide gelatin and egg yolk, continue to simmer over low heat for 2 minutes, and concentrate under reduced pressure at 60℃ to a thick paste with a relative density of 1.125 at 60℃;
[0107] The thick paste was vacuum dried at 60°C until the moisture content was less than 5%, then pulverized through a 60-mesh sieve to obtain the composition powder. The powder was then filled into gelatin capsules, with each capsule containing 0.4g, to obtain a traditional Chinese medicine composition capsule for improving sleep.
[0108] Comparative Example 2: This comparative example differs from Example 1 above in that:
[0109] Codonopsis pilosula 20 parts, Poria cocos 30 parts, Cistanche deserticola 2 parts, Angelica sinensis 5 parts, Nelumbo nucifera 3 parts, Ziziphus jujuba var. spinosa 12 parts.
[0110] Comparative Example 3: This comparative example differs from Example 1 above in that:
[0111] Instead of undergoing compound probiotic fermentation and conversion, the mixed powder obtained in step two is directly used as a substitute for the fermentation product in step four for formulation formation.
[0112] Comparative Example 4: This comparative example differs from Example 1 above in that:
[0113] In step three, the compound probiotic agent is a single Lactobacillus plantarum agent, and the inoculation amount is still 2% of the total mass of the mixed powder.
[0114] Comparative Example 5: This comparative example differs from Example 1 above in that:
[0115] The jujube seeds are not processed by honey roasting, but are taken directly as raw jujube seeds and mixed with the other processed ingredients in the same weight proportions.
[0116] Performance testing: Weigh 1g of the contents of the capsules prepared in Examples 1-3 and Comparative Examples 1-5, make up to 100mL with purified water, sonicate for 30 minutes, centrifuge and take the supernatant for later use.
[0117] GABA content determination: The content of GABA in the supernatant was determined by high performance liquid chromatography after derivatization with o-phthalaldehyde.
[0118] Total polysaccharide content determination: The phenol-sulfuric acid method was used, with glucose as a reference, to determine the total polysaccharide content in the supernatant;
[0119] In vitro simulated digestion release rate determination: The sample was incubated in simulated gastric fluid for 2 hours, then adjusted to simulated intestinal fluid and incubated for 4 hours. The concentration of total flavonoids in the supernatant before and after incubation was measured, and the release rate was calculated.
[0120] The test results are shown in Table 1.
[0121] Table 1: Detection results of the compositions in each example and comparative example
[0122] Group GABA content (mg / g) Total polysaccharide content (mg / g) Total flavonoid release rate (%) Example 1 3.28 45.6 82.4 Example 2 2.91 38.2 75.6 Example 3 3.05 42.3 79.8 Comparative Example 1 0.52 18.7 52.3 Comparative Example 2 1.86 30.5 68.2 Comparative Example 3 0.98 28.3 58.5 Comparative Example 4 1.92 32.1 70.1 Comparative Example 5 2.23 35.8 71.6
[0123] As can be seen from Examples 1 to 3 and Comparative Examples 1 to 5, and Table 1, this application can effectively improve the GABA content, total polysaccharide content and active ingredient release rate in the composition through the synergistic effect of medicinal and edible raw materials, honey processing, and compound probiotic fermentation and transformation steps, thereby achieving the effect of improving sleep.
[0124] As can be seen from Example 1 and Comparative Example 1, and Table 1, the composition prepared by honey-processing and fermentation with compound probiotics has higher GABA content and total polysaccharide content than traditional formulas, and the release rate of active ingredients is higher, indicating that this application can effectively promote the conversion and dissolution of active ingredients.
[0125] As can be seen from Example 1 and Comparative Example 2, and Table 1, when the raw material ratio exceeds the corresponding weight parts, the GABA content and total polysaccharide content in the composition decrease, and the release rate of active ingredients also decreases. This indicates that the raw material ratio range of this application can ensure the synergistic enrichment and release of active ingredients.
[0126] As can be seen from Example 1 and Comparative Example 3, and Table 1, when no compound probiotic fermentation conversion is performed, the GABA content in the composition is extremely low, and the total polysaccharide content and release rate also decrease. This indicates that the compound probiotic fermentation conversion step is a key step in promoting GABA generation and polysaccharide release, and can convert macromolecular substances in the raw materials into small molecule active ingredients.
[0127] Based on Example 1 and Comparative Example 4, and in conjunction with Table 1, it can be seen that when the compound probiotics are replaced with a single Lactobacillus plantarum, the GABA content in the composition is significantly lower than that in Example 1, and the total polysaccharide content and release rate also decrease. This indicates that the combination of Lactobacillus plantarum and Saccharomyces cerevisiae has a synergistic effect. Saccharomyces cerevisiae can assist Lactobacillus plantarum in metabolizing and producing more GABA and promote the release of polysaccharides.
[0128] Based on Example 1 and Comparative Example 5, and in conjunction with Table 1, it can be seen that when the jujube seed is not processed with honey, the GABA content and total polysaccharide content in the composition are lower than those in Example 1, and the release rate of active ingredients also decreases. This indicates that honey processing can change the physicochemical properties of jujube seed and enhance the dissolution of its active ingredients and the efficiency of subsequent fermentation and conversion.
[0129] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.
Claims
1. A composition for improving sleep and strengthening the spleen and nourishing blood, characterized in that, The ingredients include the following ingredients by weight: Codonopsis pilosula 8-16 parts, Poria cocos 12-24 parts, Cistanche deserticola 4-8 parts, Angelica sinensis 1-3 parts, Nelumbo nucifera 5-10 parts, and Ziziphus jujuba var. spinosa 4-8 parts.
2. The sleep-improving and spleen-strengthening blood-nourishing composition according to claim 1, characterized in that: The jujube kernels mentioned are honey-processed jujube kernels.
3. A method for preparing a homologous composition for improving sleep and strengthening the spleen and nourishing blood, characterized in that: The application of the sleep-improving and spleen-strengthening and blood-nourishing homologous composition according to claim 1 or 2 includes the following steps: Step 1: According to the weight proportions, honey-process the jujube seeds, and dry and grind the codonopsis, poria, cistanche, angelica and lotus seeds separately to obtain the processed raw materials; Step 2: Mix the raw materials thoroughly to obtain a mixed powder; Step 3: The mixed powder is fermented with compound probiotics to obtain fermentation products; Step 4: Formulate the fermentation product into an oral dosage form to complete the preparation.
4. The method for preparing a sleep-improving and spleen-strengthening and blood-nourishing homologous composition according to claim 3, characterized in that: In step one, the jujube seeds undergo honey-processing, which includes the following steps: Take jujube seeds, add refined honey water and mix well. Let it soak until the refined honey water is absorbed. Place it in a hot pan and stir-fry over low heat at 140-160℃ for 3-6 minutes, until the surface is slightly yellow and not sticky. The volume ratio of refined honey to water in the refined honey water is 1:0.3-1:0.5, and the weight ratio of jujube seeds to refined honey is 100:5-10.
5. The method for preparing a sleep-improving and spleen-strengthening and blood-nourishing homologous composition according to claim 3, characterized in that: In step one, the drying temperature is 40-60℃, and the drying is carried out until the moisture content is less than 10%. The particle size of the pulverized material is sieved through a 40-80 mesh sieve.
6. The method for preparing a sleep-improving and spleen-strengthening and blood-nourishing homologous composition according to claim 3, characterized in that: In step two, the mixing is carried out using a three-dimensional motion mixer with a speed of 20-40 revolutions per minute and a mixing time of 30-60 minutes.
7. The method for preparing a sleep-improving and spleen-strengthening and blood-nourishing homologous composition according to claim 3, characterized in that: Step three, the fermentation and transformation of the compound probiotics, includes the following steps: The mixed powder is placed in a fermenter, and sterile water is added at a ratio of 3 to 5 times the total mass of the mixed powder. A compound probiotic agent is inoculated, and the fermentation temperature is controlled at 30 to 37°C. The mixture is allowed to ferment under anaerobic conditions for 24 to 72 hours. The compound probiotic agent is composed of Lactobacillus plantarum and Saccharomyces cerevisiae at a mass ratio of 1.5:1 to 2.5:1, and the inoculation amount is 1% to 3% of the total mass of the mixed powder. After fermentation, the fermentation broth is heated to 80-85℃ and kept at that temperature for 25-35 minutes for inactivation treatment to obtain the fermentation product.
8. The method for preparing a sleep-improving and spleen-strengthening and blood-nourishing homologous composition according to claim 7, characterized in that: After obtaining the fermentation product, it is either spray-dried to produce fermentation powder or centrifuged and filtered to produce fermentation liquid. The inlet air temperature of the spray drying is 160-200℃ and the outlet air temperature is 80-100℃. The centrifugation speed of the centrifugation filtration is 3000-5000 rpm and the filter pore size is 0.45-1.0 micrometers.
9. The method for preparing a sleep-improving and spleen-strengthening and blood-nourishing homologous composition according to claim 3, characterized in that: In step four, the oral dosage form includes powder, pill, capsule, tablet, oral liquid or granule.
10. The method for preparing a sleep-improving and spleen-strengthening and blood-nourishing homologous composition according to claim 9, characterized in that: When the oral dosage form is a capsule, the baking powder and magnesium stearate are mixed evenly, filled into gelatin capsules, each capsule containing 0.3-0.5g, and the amount of magnesium stearate mixed is 0.5%-1.0% of the mass of the baking powder; When the oral dosage form is an oral liquid, the fermentation broth is diluted with purified water at a volume ratio of 1:1 to 1:3, and potassium sorbate at a final concentration of 0.05% to 0.1% is added as a preservative. The mixture is then filled into glass bottles, with each bottle containing 10 to 20 mL.