Hydrogel adjuvant based on chitooligosaccharide and mn3o4 nanoparticles and its use in the preparation of a foot-and-mouth disease virus-like particle vaccine

Abstract

The application discloses a kind of hydrogel adjuvant based on chitosan oligosaccharide and Mn3O4 nanoparticles and its application in preparing foot-and-mouth disease virus-like particle vaccine.The stable non-flowing gel formed by adding Mn3O4 nanoparticles in the mixed solution of chitosan oligosaccharide and oxidized hyaluronic acid.The hydrogel adjuvant based on chitosan oligosaccharide and Mn3O4 nanoparticles is loaded with calcium phosphate mineralized O-type foot-and-mouth disease virus-like particles to prepare a hydrogel vaccine.After single immunization of animals,evaluation is carried out through humoral immunity and cellular immunity,immune cell memory,antibody production library lymph node germinal center,etc.The results show that the hydrogel vaccine can activate Sting pathway,enhance antigen-presenting cell uptake,lymph node germinal center formation and sustained secretion of immune memory cells,so as to realize co-delivery of adjuvant and antigen and activate innate and adaptive immune response.The application provides a technical means with long-term immune regulation ability and long-term protection potential for preventing foot-and-mouth disease infection.

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