High-conversion-rate and high-polymer liquid grifola frondosa strain and cultivation method thereof
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHANDONG FUHE FUNGUS TECH CO LTD
- Filing Date
- 2026-06-09
- Publication Date
- 2026-07-14
Smart Images
Figure CN122381931A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of edible fungi cultivation technology, and in particular to a liquid strain of Grifola frondosa with high conversion rate and high polysaccharide content, and its cultivation method. Background Technology
[0002] Grifola frondosa, scientific name Grifola frondosa Maitake mushroom, also known as Grifola frondosa, is a rare edible fungus with high nutritional and medicinal value. Its fruiting bodies are crisp and tender with a unique flavor, and contain nutrients such as polysaccharides, proteins, amino acids, and minerals, making it highly sought after in the market. Currently, the cultivation of Grifola frondosa typically uses solid spawn, including slant culture mother cultures, primary cultures, and cultivation cultures. While solid spawn production technology is mature, the following problems still exist in actual production: 1. Solid spawn has a long propagation cycle, usually requiring multiple stages of propagation from mother culture to cultivation culture, resulting in low production efficiency; 2. Uneven mycelial distribution during inoculation of solid spawn leads to fewer germination points and slower mycelial growth; 3. Precise control of the inoculation amount is difficult, resulting in poor batch-to-batch consistency; 4. Solid spawn is prone to contamination or inconsistent mycelial age during propagation, transfer, transportation, and storage; 5. Solid spawn is not conducive to mechanized and automated inoculation, making it difficult to adapt to the needs of large-scale, factory-style production.
[0003] Liquid spawn involves culturing edible mushroom mycelium in a liquid medium with shaking or aeration, causing the mycelium to proliferate in large quantities in the form of mycelial balls, mycelial clusters, or flocculent mycelium. Liquid spawn has advantages such as strong mycelial vigor, rapid germination, uniform inoculation, and ease of quantitative and mechanized inoculation. However, *Grifola frondosa* mycelium grows relatively slowly and is quite sensitive to the nutrient composition of the culture medium, pH, dissolved oxygen, temperature, and agitation shear force. If the liquid medium formula or culture conditions are unsuitable, problems such as excessively large mycelial balls, mycelial aging, viscous spawn, decreased spawn vigor, or increased contamination rate can easily occur, thus affecting subsequent mycelial growth and fruiting results.
[0004] Therefore, it is necessary to provide a liquid spawn formula and cultivation method suitable for the growth of Grifola frondosa mycelium in order to improve the conversion rate and cultivation stability of Grifola frondosa spawn. Summary of the Invention
[0005] The purpose of this invention is to provide a liquid spawn of Grifola frondosa with high conversion rate and high polysaccharide content, and its cultivation method. By optimizing the formula of the liquid culture medium, the obtained liquid spawn has robust mycelia, good dispersibility, strong vitality, and low contamination rate. It can also shorten the mycelium growth cycle of the cultivation substrate, improve the cultivation stability of Grifola frondosa, and solve the problems of long production cycle, uneven inoculation, slow mycelium growth rate, poor mycelium age consistency, and inconvenience for mechanized inoculation of existing Grifola frondosa solid spawn.
[0006] Meanwhile, this invention improves the conversion efficiency of nutrients in the cultivation substrate to the fruiting bodies of Grifola frondosa by rapidly germinating and uniformly colonizing liquid inoculum; at the same time, by using appropriate nutrient composition of the culture medium and management conditions for mycelium growth, post-ripening, bud induction and fruiting, it promotes the accumulation of polysaccharide active ingredients in the fruiting bodies of Grifola frondosa, and obtains Grifola frondosa products with high polysaccharide content and stable quality.
[0007] To achieve the above objectives, in a first aspect, the present invention provides a high-conversion-rate, high-polysaccharide liquid strain of Grifola frondosa, wherein the liquid strain comprises Grifola frondosa mycelium and a liquid culture medium, wherein the liquid culture medium comprises 10-30 g / L glucose; 2-4 g / L yeast extract; 2-4 g / L peptone; 5-10 mL / L cabbage juice; 5-10 mL / L chestnut root extract; 0.8-1.2 g / L potassium dihydrogen phosphate; 0.2-1.0 g / L magnesium sulfate; 0.001-0.005 g / L vitamin B1; and a pH of 5.5-6.5.
[0008] Furthermore, the liquid culture medium comprises 20 g / L glucose; 3 g / L yeast extract; 2 g / L peptone; 6 mL / L cabbage juice; 6 mL / L chestnut root extract; 1.0 g / L potassium dihydrogen phosphate; 0.5 g / L magnesium sulfate; 0.002 g / L vitamin B1; and a pH of 5.8-6.0.
[0009] Furthermore, the method for preparing the cabbage juice is as follows: take fresh cabbage leaves, wash and cut them into 0.5-1 cm pieces, add distilled water in proportion, heat to 90-100℃ and keep for 20-30 minutes, cool to room temperature, first filter with 4 layers of gauze; then filter with filter paper to obtain filtrate, and then add distilled water to the filtrate to make up to the volume before heating.
[0010] Furthermore, before heating, the solid-liquid ratio of the cabbage leaves to distilled water was 1:3.
[0011] Furthermore, the method for preparing the chestnut root extract is as follows: Take fine roots from healthy chestnut trees, wash away the mud and sand, cut them into 0.5-1 cm sections, add distilled water, heat at 95-100℃ for 30 minutes, cool, filter with 4 layers of gauze, then filter with filter paper, and add distilled water to the original volume to obtain chestnut root extract.
[0012] Furthermore, before heating, the solid-liquid ratio of chestnut tree fine roots to distilled water was 1:20.
[0013] Secondly, the present invention provides a method for preparing the above-mentioned Grifola frondosa liquid culture, comprising the following steps: S1. The preserved Grifola frondosa strain is inoculated onto a solid culture medium for activation to obtain an activated strain; S2. Inoculate the activated bacterial strain into the sterilized liquid culture medium and culture with shaking to obtain the primary liquid seed; S3. Inoculate the primary liquid seed into the sterilized liquid culture medium and culture it with aeration and stirring to obtain the liquid spawn of Grifola frondosa.
[0014] Furthermore, in S2, the shaking culture conditions are: culture temperature 23-26℃, shaker speed 120-180 r / min, and culture time 5-8 days; in S3, the inoculation amount of primary liquid seed is 3%-10%, the culture temperature is 23-25℃, the aeration rate is 0.3-0.8 vvm, the stirring speed is 80-160 r / min, and the culture time is 4-7 days.
[0015] Thirdly, the present invention provides a method for cultivating Grifola frondosa, comprising the following steps: Step 1, prepare the cultivation substrate; The cultivation substrate comprises the following components by weight percentage: The substrate contains 75%-85% broadleaf tree sawdust, 10%-18% wheat bran, 3%-6% corn flour, 0.5%-1.5% gypsum, and 0.3%-1.0% lime. The moisture content of the substrate is 60%-65%, and the pH is 5.8-6.8. Step 2: Pack the cultivation substrate into bags; each bag of wet substrate weighs 1.2 kg. Step 3: Sterilize the bagged cultivation substrate; sterilization conditions are: autoclave at 121℃ for 2-3 hours, and after sterilization, cool to room temperature; Step 4: Inoculate with the Grifola frondosa liquid culture as described in any one of claims 1-6; Step 5: Perform mycelium incubation; The conditions for mycelial growth are: temperature 22-25℃, relative humidity 60%-70%, culture in the dark or with weak light, and mycelial growth time 25-40 days; Step 6: After the mycelium has grown, carry out post-ripening culture; The post-ripening culture conditions are: temperature 20-23℃, culture time 10-20 days; Step 7: Perform bud induction and mushroom production management; The conditions for inducing bud formation are: temperature 16-20℃, relative humidity 85%-95%, diffused light intensity 300-800 lx, and 8-12 hours of light per day; the conditions for managing fruiting are: temperature 16-22℃, relative humidity 85%-95%, diffused light intensity 500-1000 lx, and maintaining ventilation. Step 8: Harvest the fruiting bodies of the maitake mushroom.
[0016] Furthermore, in step 4, the inoculation amount of the Grifola frondosa liquid culture is 1%-5% of the wet weight of the cultivation substrate.
[0017] The advantages and positive effects of the high-conversion-rate, high-polysaccharide Grifola frondosa liquid strain and its cultivation method described in this invention are as follows: The liquid culture medium for Grifola frondosa provided by this invention, supplemented with cabbage juice and chestnut root extract, and formulated with appropriate proportions of glucose, yeast extract, peptone, inorganic salts, and vitamin B1, provides comprehensive nutrition and growth-promoting factors for Grifola frondosa mycelial growth, promoting the formation of uniform and vigorous mycelial balls from the liquid spawn. Using this liquid spawn for bag cultivation results in good spawn dispersion, numerous germination points, and rapid colonization and spread within the cultivation substrate, leading to uniform mycelial growth, a shorter mycelial growth cycle, and improved fruiting uniformity. Experimental results show that using the liquid spawn of this invention for bag cultivation of Grifola frondosa achieves a biological conversion rate of approximately 71.0% and a crude polysaccharide content of approximately 12.1% in the fruiting bodies, both higher than conventional solid spawn inoculation treatment and conventional liquid culture medium liquid spawn treatment. This demonstrates that the technical solution of this invention can improve the conversion efficiency of Grifola frondosa cultivation substrate, promote polysaccharide accumulation in fruiting bodies, and improve the quality of Grifola frondosa products. Furthermore, the liquid culture preparation process of this invention is simple, the mycelial balls are of suitable size, the inoculation amount is small, which facilitates quantitative inoculation and large-scale production, and is suitable for the industrialized and standardized bag cultivation of Grifola frondosa.
[0018] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description
[0019] Figure 1 The bioconversion rate of Grifola frondosa in the experimental and control groups in this embodiment of the invention; Figure 2 The crude polysaccharide content of the fruiting bodies of *Grifola frondosa* in the experimental and control groups in this embodiment of the invention. Detailed Implementation
[0020] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.
[0021] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.
[0022] Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention. Experimental methods in the following embodiments that do not specify specific conditions are generally determined according to national standards. Experimental instruments, equipment, and reagents in the following embodiments that do not specify their sources are all commercially available materials.
[0023] Unless otherwise defined or stated, all technical and scientific terms used in this invention have the same meaning as those skilled in the art. Furthermore, any methods and materials similar to or equivalent to those described herein may be applied to the methods of this invention.
[0024] The liquid culture medium in this invention includes 10-30 g / L glucose; 2-4 g / L yeast extract; 2-4 g / L peptone; 5-10 mL / L cabbage juice; 5-10 mL / L chestnut root extract; 0.8-1.2 g / L potassium dihydrogen phosphate; 0.2-1.0 g / L magnesium sulfate; 0.001-0.005 g / L vitamin B1; and a pH of 5.5-6.5.
[0025] Preferably, the liquid culture medium comprises 20 g / L glucose; 3 g / L yeast extract; 2 g / L peptone; 6 mL / L cabbage juice; 6 mL / L chestnut root extract; 1.0 g / L potassium dihydrogen phosphate; 0.5 g / L magnesium sulfate; 0.002 g / L vitamin B1; and a pH of 5.8-6.0.
[0026] The method for preparing the cabbage juice is as follows: Take fresh cabbage leaves, wash and cut them into 0.5-1 cm pieces, add distilled water in a certain proportion, heat to 90-100℃ and maintain for 20-30 minutes, cool to room temperature, first filter using 4 layers of gauze; then filter with filter paper to obtain the filtrate, and then add distilled water to the filtrate to make up to the volume before heating. Before heating, the solid-liquid ratio of cabbage leaves to distilled water is 1:3.
[0027] The method for preparing the chestnut root extract is as follows: Take fine roots from healthy chestnut trees, wash away the mud and sand, cut them into 0.5-1 cm sections, add distilled water, heat at 95-100℃ for 30 min, cool, filter through 4 layers of gauze, and then filter through filter paper. The filtrate is then replenished with distilled water to the volume before heating to obtain the chestnut root extract. Before heating, the solid-liquid ratio of chestnut root to distilled water is 1:20.
[0028] Example 1: Preparation of Grifola frondosa liquid culture A *Grifola frondosa* strain with stable fruiting characteristics and vigorous mycelial growth was selected. The preserved strain was inoculated onto PDA agar plates and cultured at 25°C in the dark for 8 days. When the mycelium covered the plate and the edges of the mycelium grew vigorously, it was used as activated strain for later use.
[0029] PDA plate culture medium, per liter of water, includes: 200g potato, 20g glucose, 18g agar, water to a final volume of 1000mL, pH adjusted to 6.0, and sterilized at 121℃ for 30 minutes.
[0030] Prepare a primary liquid culture medium. The primary liquid culture medium comprises: 20 g / L glucose; 3 g / L yeast extract; 2 g / L peptone; 6 mL / L cabbage juice; 6 mL / L chestnut root extract; 1.0 g / L potassium dihydrogen phosphate; 0.5 g / L magnesium sulfate; 0.002 g / L vitamin B1; pH 5.8-6.0. Dispense the culture medium into 250 mL Erlenmeyer flasks, 100 mL per flask, sterilize at 121°C for 30 minutes, and cool before use.
[0031] Under aseptic conditions, five mycelial blocks, each approximately 0.5-1.0 cm in size, were cut from the edge of the activated plate colonies. 2 The culture medium was inoculated into Erlenmeyer flasks. After inoculation, the flasks were placed in a shaker and cultured at 25°C and 150 rpm for 6 days to obtain primary liquid seed culture.
[0032] A secondary liquid culture medium was prepared, with the same formulation as the primary liquid culture medium. The primary liquid seed culture was inoculated into the secondary liquid culture medium at a rate of 6%, and the medium was cultured in a fermenter. The culture conditions were: temperature 24℃, aeration rate 0.5 vvm, stirring speed 120 r / min, and culture time 5 days.
[0033] After cultivation, a liquid culture of Grifola frondosa was obtained. The resulting culture was milky white, with evenly distributed mycelial balls approximately 1-2 mm in diameter. It had a pleasant fragrance, without any sour or musty odor. When the liquid culture was inoculated onto PDA plates, the mycelium resumed growth within 36 hours, indicating good viability of the liquid culture.
[0034] Example 2: Cultivation of Grifola frondosa using liquid inoculum bags Prepare the cultivation substrate. Weigh out 78% broadleaf tree wood chips, 15% wheat bran, 5% corn flour, 1% gypsum, and 1% lime by weight percentage, mix them evenly, and then add water to make the moisture content of the cultivation substrate 63%.
[0035] The cultivation substrate was packed into polypropylene cultivation bags, each bag weighing 1.2 kg of wet substrate. After packing, the bags were autoclaved at 121°C for 2.5 hours. After sterilization, the cultivation bags were cooled to below 25°C.
[0036] Under aseptic inoculation conditions, 6 mL of the Grifola frondosa liquid culture prepared in Example 1 was inoculated into each bag, and the bags were sealed after inoculation. The inoculated cultivation bags were placed in an incubation room for mycelial growth, with the incubation temperature controlled at 23-24℃ and the relative humidity controlled at 60%-70%, and cultured in the dark.
[0037] After 30-35 days of cultivation, the mycelium will have fully colonized the cultivation bag. Continue post-ripening cultivation at 20-23℃ for 12 days. Then, open the bag to induce bud formation. The conditions for bud formation are: temperature 18-20℃, relative humidity 88%-93%, diffused light intensity of about 500 lx, 10 hours of light per day, and proper ventilation.
[0038] After 7-12 days of bud induction, the primordia of Grifola frondosa will form. Continue to manage the fruiting process under conditions of 18-20℃, 85%-95% relative humidity, and diffused light. Harvest when the fruiting bodies are fully expanded but not yet mature.
[0039] Using the method of this embodiment, the liquid mycelium of Grifola frondosa germinates quickly in the cultivation medium, the mycelium grows uniformly, and the inoculation cycle is shortened compared with that of conventional solid mycelium inoculation.
[0040] Example 3: Detection of bioconversion rate and polysaccharide content 1. The method of cultivating Grifola frondosa using liquid spawn in bags as described in Example 2 was used as the experimental group. The conventional solid spawn inoculation cultivation method was used as control group 1. Liquid spawn prepared using conventional liquid culture medium was used as control group 2 and control group 3 (the liquid culture medium formula for control group 2 was: corn flour 25g / L, sucrose 25g / L, wheat bran 20g / L, yeast extract 2g / L, magnesium sulfate 2.5g / L, potassium dihydrogen phosphate 5g / L; the liquid culture medium formula for control group 3 was: potato extract, potato 200g / L; glucose 20g / L; yeast extract 3g / L; peptone 3g / L; potassium dihydrogen phosphate 1.0g / L; magnesium sulfate 0.5g / L; vitamin B1 0.005-0.01g / L). Both the experimental and control groups used the same Grifola frondosa strain, the same cultivation substrate formula, the same bagging amount, and the same management conditions for mycelial growth, post-ripening, bud induction, and fruiting. Each group was set up with several replicates. The fresh weight of the fruiting bodies and the dry weight of the cultivation substrate were counted separately, and the bioconversion rate was calculated according to the following formula: Bioconversion rate = Fresh weight of Grifola frondosa fruiting bodies / Dry weight of cultivation substrate × 100%.
[0041] 2. After harvesting mature Grifola frondosa fruiting bodies, samples were taken, dried, and pulverized. The content of Grifola frondosa polysaccharide (crude polysaccharide of fruiting body) was determined by phenol-sulfuric acid method (calculated as glucose).
[0042] Table 1. Conversion rate and crude polysaccharide content of fruiting bodies
[0043] The results are shown in Table 1 and Figure 1-2 As shown, by Figure 1It can be seen that the biological transformation rate of *Grifola frondosa* in the experimental group was 71.0%, which was higher than that of control group 1 (60.5%), control group 2 (63.5%), and control group 3 (66.4%). Compared with conventional solid inoculum treatment, the biological transformation rate of the liquid inoculum treatment of this invention increased by approximately 17.4%; compared with control groups 2 and 3, it increased by approximately 11.8% and 6.9%, respectively. This indicates that the liquid inoculum of this invention can improve the colonization ability of *Grifola frondosa* mycelium in the cultivation substrate and the substrate transformation efficiency. Figure 2 It was found that the crude polysaccharide content of the fruiting bodies of *Grifola frondosa* in the experimental group was 12.1%, which was higher than that of control group 1 (9.6%), control group 2 (10.3%), and control group 3 (10.8%). Compared with conventional solid inoculum treatment, the crude polysaccharide content of the experimental group increased by approximately 26.0%; compared with control groups 2 and 3, it increased by approximately 17.5% and 12.0%, respectively. This indicates that cultivation using the liquid inoculum of *Grifola frondosa* prepared using the liquid culture medium of this invention is beneficial for increasing the accumulation of polysaccharide active ingredients in the fruiting bodies of *Grifola frondosa*.
[0044] The test results show that, compared with conventional solid inoculation and conventional liquid culture medium preparation of liquid inoculum, the liquid inoculum of the present invention germinates faster in the cultivation substrate and grows mycelium more uniformly, which is beneficial to improving the conversion efficiency of Grifola frondosa cultivation substrate, and at the same time has a certain promoting effect on the accumulation of polysaccharides in fruiting bodies.
[0045] Therefore, this invention optimizes the liquid culture medium formula, resulting in liquid spawn with robust mycelia, good dispersibility, high vitality, and low contamination rate. It also shortens the mycelial growth cycle of the cultivation substrate, improves the stability of Grifola frondosa cultivation, and solves problems such as long production cycles, uneven inoculation, slow mycelial growth, poor mycelial age consistency, and inconvenience for mechanized inoculation associated with existing solid Grifola frondosa spawn. Simultaneously, this invention improves the conversion efficiency of nutrients in the cultivation substrate to Grifola frondosa fruiting bodies through rapid germination and uniform colonization of the liquid spawn. Furthermore, by using suitable nutrient composition of the culture medium and appropriate management conditions for mycelial growth, post-ripening, bud induction, and fruiting, it promotes the accumulation of polysaccharide active ingredients in Grifola frondosa fruiting bodies, resulting in Grifola frondosa products with high polysaccharide content and stable quality.
[0046] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.
Claims
1. A liquid strain of Grifola frondosa with high conversion rate and high polysaccharide content, characterized in that: The Grifola frondosa liquid culture medium comprises Grifola frondosa mycelium and liquid culture medium, wherein the liquid culture medium comprises 10-30 g / L glucose; 2-4 g / L yeast extract; 2-4 g / L peptone; 5-10 mL / L cabbage juice; 5-10 mL / L chestnut root extract; 0.8-1.2 g / L potassium dihydrogen phosphate; 0.2-1.0 g / L magnesium sulfate; 0.001-0.005 g / L vitamin B1; and a pH of 5.5-6.
5.
2. The high-conversion-rate, high-polysaccharide Grifola frondosa liquid strain according to claim 1, characterized in that: The liquid culture medium comprises 20 g / L glucose; 3 g / L yeast extract; 2 g / L peptone; 6 mL / L cabbage juice; 6 mL / L chestnut root extract; 1.0 g / L potassium dihydrogen phosphate; 0.5 g / L magnesium sulfate; 0.002 g / L vitamin B1; and a pH of 5.8-6.
0.
3. The high-conversion-rate, high-polysaccharide Grifola frondosa liquid strain according to claim 2, characterized in that, The method for preparing the cabbage juice is as follows: Take fresh cabbage leaves, wash and cut them into 0.5-1 cm pieces, add distilled water in proportion, heat to 90-100℃ and keep for 20-30 minutes, cool to room temperature, first filter with 4 layers of gauze; then filter with filter paper to obtain filtrate, and then add distilled water to the filtrate to make up to the volume before heating.
4. The high-conversion-rate, high-polysaccharide Grifola frondosa liquid strain according to claim 3, characterized in that: Before heating, the solid-liquid ratio of cabbage leaves to distilled water was 1:
3.
5. The high-conversion-rate, high-polysaccharide Grifola frondosa liquid strain according to claim 2, characterized in that, The method for preparing the chestnut root extract is as follows: Take fine roots from healthy chestnut trees, wash away the mud and sand, cut them into 0.5-1 cm sections, add distilled water, heat at 95-100℃ for 30 minutes, cool, filter with 4 layers of gauze, then filter with filter paper, and add distilled water to the original volume to obtain chestnut root extract.
6. The Grifola frondosa liquid strain with high conversion rate and high polysaccharide content according to claim 5, characterized in that: Before heating, the solid-liquid ratio of chestnut tree fine roots to distilled water was 1:
20.
7. The method for preparing the liquid mycelium of Grifola frondosa according to any one of claims 1-6, characterized in that, Includes the following steps: S1. The preserved Grifola frondosa strain is inoculated onto a solid culture medium for activation to obtain an activated strain; S2. Inoculate the activated bacterial strain into the sterilized liquid culture medium and culture with shaking to obtain the primary liquid seed; S3. Inoculate the primary liquid seed into the sterilized liquid culture medium and culture it with aeration and stirring to obtain the liquid spawn of Grifola frondosa.
8. The preparation method according to claim 7, characterized in that, In S2, the shaking culture conditions are: culture temperature 23-26℃, shaking speed 120-180 r / min, and culture time 5-8 days; in S3, the inoculation amount of primary liquid seed is 3%-10%, the culture temperature is 23-25℃, the aeration rate is 0.3-0.8 vvm, the stirring speed is 80-160 r / min, and the culture time is 4-7 days.
9. A method for cultivating Grifola frondosa, characterized in that, Includes the following steps: Step 1, prepare the cultivation substrate; The cultivation substrate comprises the following components by weight percentage: The substrate contains 75%-85% broadleaf tree sawdust, 10%-18% wheat bran, 3%-6% corn flour, 0.5%-1.5% gypsum, and 0.3%-1.0% lime. The moisture content of the substrate is 60%-65%, and the pH is 5.8-6.
8. Step 2: Pack the cultivation substrate into bags; each bag of wet substrate weighs 1.2 kg. Step 3: Sterilize the bagged cultivation substrate; The sterilization conditions are: autoclaving at 121℃ for 2-3 hours, followed by cooling to room temperature after sterilization. Step 4: Inoculate with the Grifola frondosa liquid culture as described in any one of claims 1-6; Step 5: Perform mycelium incubation; The conditions for mycelial growth are: temperature 22-25℃, relative humidity 60%-70%, culture in the dark or with weak light, and mycelial growth time 25-40 days; Step 6: After the mycelium has grown, carry out post-ripening culture; The post-ripening culture conditions are: temperature 20-23℃, culture time 10-20 days; Step 7: Perform bud induction and mushroom production management; The conditions for inducing bud formation are: temperature 16-20℃, relative humidity 85%-95%, diffused light intensity 300-800 lx, and 8-12 hours of light per day; the conditions for managing fruiting are: temperature 16-22℃, relative humidity 85%-95%, diffused light intensity 500-1000 lx, and maintaining ventilation. Step 8: Harvest the fruiting bodies of the maitake mushroom.
10. The cultivation method according to claim 9, characterized in that: In step 4, the inoculation amount of Grifola frondosa liquid spawn is 1%-5% of the wet weight of the cultivation substrate.