Genetically engineered coryneform bacteria for producing porphyrin-334 and use thereof

By genetically engineering Corynebacterium glutamicum, knocking out competing pathways and heterologously expressing key enzyme gene clusters, the problem of the difficulty in obtaining Porphyra-334 naturally was solved, and efficient production was achieved, reaching an accumulation of 0.77 g/L in shake flasks and 4.8 g/L in fermenters.

CN122381985APending Publication Date: 2026-07-14
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Filing Date
2026-06-05
Publication Date
2026-07-14

AI Technical Summary

Technical Problem

In the existing technology, the natural acquisition of Porphyra-334 is difficult and the content is low, making it difficult to achieve efficient production.

Method used

By genetically engineering Corynebacterium glutamicum, knocking out the tal gene, which encodes the transaldase, through the competitive pathway of sedulose-7-phosphate, and heterologously expressing four key enzyme gene clusters from Nostoc linyi NIES-25, a genetically engineered strain was constructed. This strain enhanced the expression of phosphoglycerate kinase and pyruvate kinase, improved ATP regeneration capacity, and achieved efficient synthesis of Porphyra-334.

Benefits of technology

The accumulation of Porphyra-334 reached 0.77 g/L in shake flasks and 4.8 g/L in a 50 L fermenter, providing an efficient and green production method.

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Abstract

The application discloses a genetically engineered corynebacterium glutamicum for producing phycobilisome amino acid Porphyra-334 and application thereof, and belongs to the field of genetic engineering and fermentation technology. The genetically engineered corynebacterium glutamicum takes corynebacterium glutamicum ATCC 13032 as a host strain, competes with a path gene by knocking out, introduces four key synthesis pathway enzyme genes for synthesizing Porphyra-334 from Nostoc linckia NIES-25, and synthesizes the key enzyme genes of the synthesis pathway in multiple copies on the genome, so that the synthesis yield of Porphyra-334 in the microbial cell is improved. The strain produces Porphyra-334 in a shaking flask, and 0.77 g / L of Porphyra-334 can be accumulated in 72 h. In fed-batch fermentation in a 50 L fermenter, the strain can be cultured to 4.8 g / L.
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