A chemically defined serum-free medium suitable for three-dimensional culture of mesenchymal stem cells
By adding specific components to MSC culture medium to construct a chemically defined serum-free culture medium, the animal-derived risks of existing culture media and the need for large-scale three-dimensional culture were addressed, achieving efficient amplification of MSCs and stability of their immunophenotype.
CN122382001APending Publication Date: 2026-07-14GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD +1
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
- Filing Date
- 2026-06-04
- Publication Date
- 2026-07-14
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Figure CN122382001A_ABST
Abstract
The application belongs to the technical field of cell culture medium, and particularly relates to a chemical composition limited type serum-free culture medium suitable for three-dimensional culture of mesenchymal stem cells. The culture medium takes DMEM / F12 as a basic culture medium, adds recombinant human insulin, recombinant human EGF, recombinant human b-FGF, recombinant human PDGF-BB and MEM NEAA as growth promoting components; selects L-glutathione and 2-mercaptoethanol as antioxidants; adopts recombinant human blood albumin and polyvinyl alcohol as anti-shear protective agents; takes recombinant human blood coagulation factor II and recombinant human type I collagen as adhesion promoting factors, and adds recombinant human furin and recombinant human Wnt-3a as activating factors. The culture medium can realize efficient expansion of MSC in a three-dimensional culture system of a bioreactor microcarrier, solve the problem of insufficient cell quantity, and maintain the biological characteristics and immunophenotype stability of mesenchymal stem cells.
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