Gfp-tagged infectious bursal disease virus with vp1 sumoylation and ubiquitination double site mutation, construction method and application thereof
By constructing an I404T/K290R dual-site mutant virus of the infectious bursal disease virus VP1 protein, the problem of lacking a viral model to study the synergistic regulation of SUMOylation and ubiquitination in existing technologies has been solved, and the viral replication capacity has been partially restored, providing a foundation for novel attenuated live vaccines.
CN122382015APending Publication Date: 2026-07-14JIANGXI AGRICULTURAL UNIVERSITY
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- JIANGXI AGRICULTURAL UNIVERSITY
- Filing Date
- 2026-04-07
- Publication Date
- 2026-07-14
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Figure CN122382015A_ABST
Abstract
The application discloses a kind of infectious bursal disease virus VP1 SUMO and ubiquitination double-site mutant virus, construction method and application thereof, belong to molecular virology technical field.The amino acid sequence of the VP1 protein of the virus includes I404T and K290R double-site mutation.Experiments prove that the double mutant virus significantly reduces the excessive ubiquitination level caused by SUMO deletion, its replication ability is between wild type virus and SUMO deletion single mutant virus, and has good genetic stability.The recombinant virus provided in the application provides an important tool for studying the synergistic regulation mechanism of IBDV protein post-translational modification, and can be used in the prevention of avian infectious bursal disease as attenuated live vaccine candidate strain.
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