Fermentation preparation process of cultivating bovine bezoar in vitro

By using a probiotic engineered bacteria fermentation process, the problem of excessive free bilirubin in the preparation of in vitro cultured bezoar has been solved, resulting in a highly efficient and stable in vitro cultured bezoar suitable for industrial production and possessing excellent medicinal efficacy.

CN122382103APending Publication Date: 2026-07-14CHINA PHARM UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-13
Publication Date
2026-07-14

AI Technical Summary

Technical Problem

In existing technologies, the free bilirubin content in the preparation of in vitro cultured bezoar exceeds the standard, making it difficult to meet the requirements of the 2020 edition of the Chinese Pharmacopoeia. Furthermore, the preparation process is complex and not suitable for industrial production.

Method used

Using a probiotic engineered bacteria fermentation process, probiotics producing β-G-enzymes are constructed. Fermentation is carried out using specific temperatures and environments to simulate the internal environment of the bovine gallbladder. Specific drug components are added to prepare bilirubin calcium that does not contain free bilirubin. Combined with a one-step fermentation culture, spherical solids are produced and freeze-dried to produce in vitro cultured bezoar.

Benefits of technology

The method has achieved efficient and stable in vitro cultured bezoar preparation with a bilirubin content of 80%, which meets the pharmacopoeia requirements. It has antipyretic and anticonvulsant effects and is suitable for industrial production.

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Abstract

The application discloses a fermentation preparation process of in-vitro cultivated bovine bezoar, and mainly uses natural bovine bile, combined bilirubin salt and fixed additive ingredients as main raw materials to prepare the in-vitro cultivated bovine bezoar by one-step fermentation. The application solves the technical problem of excessive free bilirubin in the in-vitro cultivated bovine bezoar in the prior art, and the process can cultivate the safe and effective in-vitro cultivated bovine bezoar with up-to-standard quality by one-step fermentation, and is simple and convenient, stable in process and capable of batch production.
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Citation Information

Patent Citations

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  • Method for preparing composite calcium bilirubinate from ox gall

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