A method for rapidly detecting streptococcus suis or / and salmonella based on RAA-EXO technology and application thereof

Abstract

This invention discloses a method for rapid detection of Streptococcus suis and / or Salmonella based on RAA-EXO technology and its application. This method targets Streptococcus suis. recN Genes and Salmonella invA Genes were used to design and screen specific RAA primers and EXO probes, with sequences shown in SEQ ID NO. 3, 5, 10 and SEQ ID NO. 14, 15, 20, respectively. Single or double RAA-EXO detection systems for Streptococcus suis and Salmonella were established. Detection can be completed within 20 minutes under constant temperature conditions of 39-41℃, and no cross-reactivity is observed with common bacteria such as Escherichia coli and Staphylococcus aureus. Results can be visually determined by fluorescence curves or blue light analysis. This provides an effective tool for rapid, sensitive, and on-site detection of Streptococcus suis and Salmonella, and has significant application value in food safety and disease control.

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