Hair care composition

Abstract

The present invention provides a hair care composition comprising: (i) taxifolin glucoside; (ii) N-acetyl tyrosine; (iii) at least one yeast peptide hydrolysate of the species Saccharomyces cerevisiae; (iv) at least one zinc salt. The present invention also provides uses and methods comprising the hair care composition of the present invention.

Description

Technical Field

[0001] This invention relates to hair care compositions, particularly anti-gray hair care compositions. Background Technology

[0002] As people age, the appearance of their hair may become less desirable. Graying and whitening of hair are clear signs of aging. Hair also loses its elasticity, becoming thinner and more fragile. In addition, the diameter of hair follicles and the number of hairs decrease, resulting in a sparse appearance of the scalp.

[0003] For many consumers, maintaining hair color and good thickness, fullness, and quantity is a way to mitigate signs of aging. Many people choose to dye their hair to cover up age-related graying or whitening. However, hair dyeing does not help conceal other age-related changes in hair quality. On the contrary, chemical hair dyes are known to cause increased hair damage over time.

[0004] Therefore, EP3873483A1 describes a hair care surfactant containing taxifolin glucoside and N-acetyltyrosine, which can provide some reduction in the appearance of gray and white hair in an individual. In EP3873483A1, the hair care surfactant was found to reduce the number of white hairs by an average of 17% after 4 months.

[0005] However, despite the existence of such existing technologies, there has long been a need to provide consumers with compositions that effectively reduce age-related functional changes in hair pigmentation. There is also a need for a composition that can also reduce or prevent other harmful changes in age-related hair.

[0006] Surprisingly, it was found that when taurine glucoside and N-acetyrosine were combined with specific hydrolyzed yeast proteins and zinc salts from the Saccharomyces cerevisiae species, they could effectively reduce age-related functional changes in hair pigmentation, while also providing beneficial improvements in more age-related hair characteristics.

[0007] In particular, it was surprisingly found that yeast peptide hydrolysates containing *Saccharomyces cerevisiae* species can improve the antioxidant activity of hair care compositions. Specifically, when taurine glucoside and N-acetyltyrosine are combined with yeast peptide hydrolysates of *Saccharomyces cerevisiae* species according to the present invention, the antioxidant activity of taurine glucoside and N-acetyltyrosine in the hair care composition can be enhanced, and oxidative stress in melanocytes in the scalp / hair follicles can be reduced.

[0008] Increased antioxidant activity in melanocytes is associated with reduced gray hair formation and / or delayed regrowth. Oxidative stress is known to damage melanocytes, leading to graying of hair. Enhanced antioxidant activity helps neutralize reactive oxygen species (ROS), thereby reducing oxidative damage to melanocytes and gray hair formation. Furthermore, enhanced antioxidant defense better protects melanocytes and their precursor cells in the hair follicles of the scalp from oxidative stress. This protection helps maintain melanocyte function, lifespan, and melanin production for longer periods. Increased antioxidant activity is also known to be associated with the restoration of pigmentation in gray hair caused by reduced ROS levels, protection of the melanocyte pool, and reactivation of melanin production.

[0009] In addition, reducing oxidative stress in the scalp / hair follicles is also associated with improving other age-related hair characteristics, such as reducing hair loss and / or enhancing the growth of stronger hair. Summary of the Invention

[0010] In a first aspect, the present invention relates to a hair care composition comprising: (i) Taxonitrile glucoside; (ii) N-acetyltyrosine; (iii) at least one yeast peptide hydrolysate of a *Saccharomyces cerevisiae* species; and (iv) At least one zinc salt.

[0011] In a further aspect, the present invention relates to the non-therapeutic use of the hair care compositions of the present invention for reducing the appearance of gray or white hair in an individual, for reducing age-related functional changes in hair pigmentation, for increasing melanin production in gray hair, for increasing cell proliferation, for increasing melanocyte proliferation, for increasing tyrosinase levels and / or activity, for increasing hair volume, for increasing hair thickness, for increasing hair count, for increasing hair strength, for reducing hair loss and / or for hair fiber repair, for improving scalp health, for reducing scalp inflammation, for reducing oxidative stress on the scalp, for reducing oxidized lipid substances on the scalp, for improving sebum control and / or for improving the scalp barrier.

[0012] In a further aspect, the present invention relates to a method for reducing the occurrence of gray or white hair in an individual, reducing age-related functional changes in hair pigmentation, increasing melanin production in gray hair, increasing cell proliferation, increasing melanocyte proliferation, increasing tyrosinase levels and / or activity, increasing hair volume, increasing hair thickness, increasing hair count, increasing hair strength, reducing hair loss, providing hair fiber repair, improving scalp health, reducing scalp inflammation, reducing oxidative stress on the scalp, reducing oxidized lipid substances on the scalp, improving sebum control, and / or improving the scalp barrier, said method comprising applying the hair care composition of the present invention to the scalp or hair.

[0013] Preferably, the present invention provides a non-therapeutic use of the hair care composition of the present invention for reducing oxidative stress in melanocytes, preferably in hair follicles and / or melanocytes in the scalp. Preferably, the present invention provides a non-therapeutic use of the hair care composition of the present invention for reducing oxidized lipid substances on the scalp.

[0014] Preferably, the present invention provides a method for reducing oxidative stress in melanocytes, preferably in hair follicles and / or scalp, the method comprising applying the hair care composition of the present invention to the scalp or hair. Preferably, the present invention provides a method for reducing oxidized lipid substances on the scalp, the method comprising applying the hair care composition of the present invention to the scalp or hair.

[0015] Surprisingly, the composition of the hair care composition of the present invention provides a reduction in the appearance of gray or white hair in an individual. In some embodiments, the composition of the hair care composition provides a synergistic reduction in gray or white hair in an individual. In some embodiments, the composition of the hair care composition of the present invention provides a reduction in age-related functional changes in hair pigmentation. In some embodiments, the composition of the hair care composition provides a synergistic reduction in age-related functional changes in hair pigmentation. For example, advantageously, the hair care composition of the present invention can provide increased melanin production, such as a synergistic increase in melanin production. The hair care composition of the present invention advantageously provides an increase in cell proliferation, such as an increase in melanocyte proliferation, such as a synergistic increase in melanocyte proliferation. The hair care composition of the present invention advantageously provides increased tyrosinase levels and / or activity, such as a synergistic increase in tyrosinase levels and / or activity.

[0016] The beneficial effects of the hair care compositions of the present invention can surprisingly lead to a noticeable reduction in high levels of age-related functional changes in individual hair pigmentation and / or the appearance of gray or white hair. For example, the compositions according to the invention can show a reduction of more than 22% in gray hair after only 2 months of application and a reduction of more than 24% in gray hair after only 3 months of application.

[0017] In addition to reducing age-related functional changes in hair pigmentation, the composition of the hair care compositions of the present invention provides beneficial improvements in other age-related hair properties. For example, the hair care compositions of the present invention can provide a beneficial increase in hair volume, such as a synergistic increase in hair volume. For example, after one month of application of the compositions according to the present invention, 90% of subjects showed an increase in hair volume. The hair care compositions of the present invention can provide a beneficial increase in hair thickness, such as a synergistic increase in hair thickness. For example, after only one month of application of the compositions according to the present invention, 93% of subjects showed an increase in hair volume. The hair care compositions of the present invention can provide a beneficial increase in hair count, such as a synergistic increase in hair count. For example, after only one month of application of the compositions according to the present invention, 93% of subjects showed an improvement in hair count. The hair care compositions of the present invention can provide a beneficial increase in hair strength / reduction in hair loss, such as a synergistic increase in hair strength / reduction in hair loss. For example, after only three months of application of the compositions according to the present invention, a reduction of more than 70% in the count of intact / broken hairs in the brush was observed. The hair care composition of the present invention can also provide observable fiber repair, such as synergistic fiber repair.

[0018] In addition to reducing age-related functional changes in hair pigmentation, the composition of the hair care composition of the present invention also provides beneficial improvements in scalp health. For example, the hair care composition of the present invention can provide a beneficial reduction in scalp inflammation, such as a synergistic reduction in scalp inflammation. The hair care composition of the present invention can also provide a beneficial scavenging of reactive oxygen species (ROS), thereby leading to a reduction in oxidative stress on the scalp, such as a synergistic reduction in oxidative stress on the scalp. The hair care composition of the present invention can also provide a beneficial reduction in oxidized lipids on the scalp, such as a synergistic reduction in oxidized lipids on the scalp. The hair care composition of the present invention can also provide a beneficial improvement in sebum control, such as a synergistic improvement in sebum control. The hair care composition of the present invention can also provide beneficial improvements in scalp health and barrier function, such as a synergistic improvement in scalp health and barrier function. Detailed Implementation

[0019] The aspects and embodiments of the invention will now be discussed. Other aspects and embodiments will be apparent to those skilled in the art. All references herein are incorporated by way of citation.

[0020] In this invention, the term "extract" refers to any isolated substance obtained by extraction from a raw material. For example, a yeast extract may contain peptides or oligopeptides isolated from a yeast raw material. Extracts are obtained, for example, by dissolving the raw material in a solvent (e.g., water or alcohol) and then concentrating and / or purifying the substance isolated from the raw material, for example, by evaporating the solvent.

[0021] "Peptide hydrolysis products" should be understood as a mixture of compounds consisting mostly of peptides or oligopeptides obtained by hydrolyzing peptide bonds in proteins, peptides, or oligopeptides.

[0022] (i) Taxonitrogenin glucoside The compositions of the present invention contain taurine glucoside.

[0023] Preferably, at least a portion of the taxonomyl glucoside is taxonomyl α-D-glucoside. A suitable method for preparing taxonomyl α-D-glucoside is described in WO 2007 / 144368.

[0024] In a preferred embodiment, the hair care composition of the present invention further comprises taurine.

[0025] For example, a mixture of taxine and taxine glucoside can be prepared by stopping the reaction according to WO 2007 / 144368 before complete conversion, for example, after about half of the taxine has been converted to obtain a mixture of approximately 1:1.

[0026] Taxifenin glucoside and taxifenin may be present in a weight ratio of 100:0 to about 40:60. Preferably, the weight ratio of taxifenin glucoside to taxifenin is 90:10 to 40:60, more preferably 70:30 to 50:50, and most preferably about 60:40.

[0027] The amount of taurine glucoside in the hair care composition of the present invention may be, for example, 0.0001% to 0.50% of the total weight of the composition, more preferably 0.0005% to 0.025% of the total weight of the composition, more preferably 0.0007% to 0.009% of the total weight of the composition, such as 0.0009% to 0.007% of the total weight of the composition, such as 0.001% to 0.004% of the total weight of the composition, such as 0.001% to 0.002% of the total weight of the composition.

[0028] In the hair care composition of the present invention, a portion of the taurine glucoside may be replaced by taurine. In particular, up to 60%, preferably up to 50%, and even more preferably up to 40% of the taurine glucoside may be replaced by taurine.

[0029] In some embodiments, the taxonomic glucoside and / or taxonomic glycoside of the present invention can be derived from European larch (Larix Europaea Wood). For example, taxonomic glucoside and / or taxonomic glycoside can be contained in European larch extract.

[0030] The amount of European larch extract in the composition is, for example, 0.0001% to 0.1% of the total weight of the composition, more preferably 0.001% to 0.01% of the total weight of the composition, and most preferably 0.002% to 0.008% of the total weight of the composition.

[0031] (ii) N-acetyltyrosine The compositions of the present invention contain N-acetyltyrosine.

[0032] The amount of N-acetyltyrosine in the hair care composition of the present invention can be, for example, 0.01% to 30% of the total weight of the composition, more preferably 0.05% to 20% of the total weight of the composition, more preferably 0.1% to 5% of the total weight of the composition, and most preferably 0.15% to 0.9% of the total weight of the composition, for example 0.15% to 0.4% of the total weight of the composition.

[0033] In the hair care composition of the present invention, the weight ratio of taurine glucoside to N-acetyltyrosine is preferably 1:500 to 1:1, more preferably 1:300 to 1:50, and most preferably 1:200 to 1:100, for example about 1:150.

[0034] Compositions comprising taxine glucoside and / or N-acetyltyrosine can be as described, for example, in EP3873483A1 (incorporated herein by reference). Taxine glucoside and / or N-acetyltyrosine can also be supplied to the compositions of the present invention by means of the commercially available material Darkenyl™ from Givaudan.

[0035] (iii) Yeast extract The composition contains at least one yeast peptide hydrolysate of a Saccharomyces cerevisiae species.

[0036] Preferably, the peptide hydrolysate is a low molecular weight peptide hydrolysate. More preferably, the peptide hydrolysate has a molecular weight of 6 kDa or less, more preferably 5 kDa or less, even more preferably 3 kDa or less, such as 1 kDa or less, and most preferably has a molecular weight of less than 600 Da. The molecular weight of the peptide hydrolysate according to the invention can be identified using high-performance liquid chromatography (HPLC) and suitable solvent systems / gradients known in the art.

[0037] Preferably, yeast peptide hydrolysates from the *Saccharomyces cerevisiae* species are prepared by hydrolyzing yeast proteins derived from the *Saccharomyces cerevisiae* species. For example, yeast extracts can be prepared by extracting yeast proteins and then performing controlled hydrolysis (releasing peptides and protein fragments).

[0038] Any extraction or purification method known to those skilled in the art can be used to prepare the extract according to the present invention.

[0039] Preferably, the yeast peptide hydrolysate of the Saccharomyces cerevisiae species is an aqueous extract. The extract disclosed herein is preferably a purified aqueous extract.

[0040] In some embodiments of the invention, the yeast peptide hydrolysate contains 30% to 70% of the total weight of the dry peptide hydrolysate, and more particularly, the amount is 40% to 50% of the total weight of the dry extract.

[0041] The amount of yeast peptide hydrolysate in the hair care composition of the present invention can be, for example, 0.0001% to 20% of the total weight of the composition, and preferably 0.003% to 5% of the total weight of the composition, more preferably 0.005% to 0.1% of the total weight of the composition, and even more preferably 0.005% to 0.01% of the total weight of the composition, for example, about 0.006 to 0.009% by weight.

[0042] Therefore, for yeast peptide hydrolysates containing 30% to 70% peptide / protein fragment compounds, the content of protein and peptide compounds in the composition of the present invention is 0.00003% to 14% of the total weight of the composition, and preferably 0.0009% to 3.5% of the total weight of the composition, more preferably 0.0015% to 0.07% of the total weight of the composition, even more preferably 0.0015% to 0.007% of the total weight of the composition, for example 0.0018% to 0.0063% of the total weight of the composition.

[0043] Therefore, for yeast peptide hydrolysates containing 40% to 50% peptide / protein fragment compounds, the content of protein and peptide compounds in the composition of the present invention is 0.00004% to 10% of the total weight of the composition, and preferably a concentration of 0.0012% to 2.5% of the total weight of the composition, more preferably a concentration of 0.002% to 0.05% of the total weight of the composition, even more preferably a concentration of 0.002% to 0.005% of the total weight of the composition, for example 0.0024% to 0.0045% by weight.

[0044] Yeast peptide hydrolysates of the *Saccharomyces cerevisiae* species can be as described, for example, in US2013296251A1 (incorporated herein by reference). Yeast peptide hydrolysates of the *Saccharomyces cerevisiae* species can also be provided to the compositions of the present invention using Dynagenl™, a commercially available material from Ashland.

[0045] Preferably, the ratio of yeast peptide hydrolysate to N-acetyltyrosine is 1:0.5 to 1:100, more preferably 1:1 to 1:50, and even more preferably 1:1:5 to 1:30. In some embodiments, the ratio of yeast peptide hydrolysate to N-acetyltyrosine is 1:10 to 1:25.

[0046] Preferably, the ratio of yeast peptide hydrolysate to taxine glucoside is 1:0.001 to 1:5, more preferably 1:0.01 to 1:3, and even more preferably 1:0.05 to 1:1. In some embodiments, the ratio of yeast peptide hydrolysate to taxine glucoside is 1:0.1 to 1:0.5.

[0047] Preferably, the ratio of yeast peptide hydrolysate to European larch extract is 1:0.001 to 1:5, more preferably 1:0.01 to 1:3, and even more preferably 1:0.05 to 1:1. In some embodiments, the ratio of yeast peptide hydrolysate to European larch extract is 1:0.1 to 1:0.8.

[0048] (iv) Zinc salts The hair care composition of the present invention also contains one or more zinc salts. Zinc can act as an inhibitor of enzymes associated with hair loss. Therefore, enzyme inhibition can advantageously lead to a reduction in hair loss or thinning. The inclusion of zinc salts in the compositions of the present invention can help provide a beneficial reduction in age-related functional changes in hair pigmentation and a beneficial improvement in other age-related hair properties.

[0049] The zinc salts applicable to this invention include zinc sulfate, zinc gluconate, zinc chloride, zinc pyrithione, or combinations thereof.

[0050] Most preferably, the composition comprises zinc sulfate.

[0051] The amount of zinc salt in the hair care composition of the present invention can be, for example, 0.00001% to 5% of the total weight of the composition, and preferably 0.0001% to 3% of the total weight of the composition, more preferably 0.001% to 1% of the total weight of the composition, even more preferably 0.01% to 0.1% of the total weight of the composition, and most preferably 0.01% to 0.05% of the total weight of the composition, for example, up to 0.03%.

[0052] Preferably, the amount of zinc sulfate in the hair care composition of the present invention can be, for example, 0.00001% to 5% of the total weight of the composition, and more preferably 0.0001% to 3% of the total weight of the composition, more preferably 0.001% to 1% of the total weight of the composition, more preferably 0.01% to 0.1% of the total weight of the composition, and most preferably 0.01% to 0.05% of the total weight of the composition, for example, up to 0.03%.

[0053] Preferably, the amount of zinc gluconate in the hair care composition of the present invention may be, for example, 0.00001% to 5% of the total weight of the composition, and more preferably 0.00001% to 3% of the total weight of the composition, more preferably 0.00005% to 1% of the total weight of the composition, more preferably 0.00005% to 0.1% of the total weight of the composition, and most preferably 0.00005% to 0.05% of the total weight of the composition, for example, about 0.00008% to 0.0005% of the total weight of the composition.

[0054] Preferably, the amount of zinc chloride in the hair care composition of the present invention can be, for example, 0.00001% to 5% of the total weight of the composition, and more preferably 0.0001% to 3% of the total weight of the composition, more preferably 0.0005% to 1% of the total weight of the composition, more preferably 0.0005% to 0.1% of the total weight of the composition, and most preferably 0.0005% to 0.04% of the total weight of the composition, for example, about 0.0008% to 0.03% of the total weight of the composition.

[0055] Preferably, the weight ratio of yeast peptide hydrolysate to zinc salt is 1:0.01 to 1:10; more preferably, the weight ratio is 1:0.1 to 1:5; and even more preferably, 1:0.15 to 1:3. In some embodiments, the weight ratio is 1:1 to 1:2.5.

[0056] Preferably, the weight ratio of yeast peptide hydrolysate to zinc sulfate is 1:0.01 to 1:10; more preferably, the weight ratio is 1:0.1 to 1:5; and even more preferably, 1:0.15 to 1:3. In some embodiments, the weight ratio of yeast extract to zinc sulfate is 1:1 to 1:2.5.

[0057] In a preferred embodiment, the hair care composition comprises: (i) 0.0005% to 0.25% of piperidine glucoside; (ii) 0.05% to 20% N-acetyltyrosine; (iii) 0.001% to 5% of yeast peptide hydrolysates of the *Saccharomyces cerevisiae* species; and (iv) 0.001% to 0.1% zinc salts.

[0058] In a preferred embodiment, the hair care composition comprises: (i) 0.0005% to 0.25% of piperidine glucoside; (ii) 0.05% to 20% N-acetyltyrosine; (iii) 0.001% to 1% of yeast peptide hydrolysates of the *Saccharomyces cerevisiae* species; and (iv) 0.001% to 0.1% zinc salts.

[0059] Most preferably, the hair care composition comprises: (i) 0.0007% to 0.009% of taxine glucoside; (ii) 0.1% to 5% N-acetyltyrosine; (iii) 0.003% to 0.1% of yeast peptide hydrolysates of the *Saccharomyces cerevisiae* species; and (iv) 0.01% to 0.1% zinc sulfate.

[0060] Product form and other ingredients The compositions used in this invention (as described above) may include other active substances for improving the physical and / or aesthetic properties of the scalp and / or hair. Examples include amino acids, vitamins, minerals and / or antioxidants, emollients, moisturizers, sunscreens, anti-irritants, exfoliants, plant extracts (such as pomegranate, birch, green tea, chamomile and licorice extracts) and mixtures thereof, preservatives (such as sodium benzoate, potassium sorbate, phenoxyethanol, pentylene glycol and sodium metabisulfite), pH adjusters and fragrances (such as essential oils, flower oils, natural extracts from resins, gums, balsams, legumes, mosses and other plants, as well as synthetic aromatic materials).

[0061] In some embodiments, the compositions of the present invention preferably further comprise a thickener. Suitable thickeners are selected from polyacrylic acid, crosslinked polymers of acrylic acid, copolymers of acrylic acid with hydrophobic monomers, copolymers of carboxylic acid-containing monomers with acrylates, crosslinked copolymers of acrylic acid with acrylates, heteropolysaccharide gums, and crystalline long-chain acrylic derivatives. Long-chain acrylic derivatives are ideally selected from ethylene glycol stearate, alkanolamides of fatty acids having 16 to 22 carbon atoms, and mixtures thereof. Ethylene glycol distearate and polyethylene glycol 3-distearate are preferred long-chain acrylic derivatives because they impart pearlescent properties to the composition. Polyacrylic acid is commercially available as Carbopol 420, Carbopol 488, or Carbopol 493. Polymers of acrylic acid crosslinked with multifunctional agents can also be used; these are commercially available as Carbopol 910, Carbopol 934, Carbopol 941, and Carbopol 980. An example of a suitable copolymer of carboxylic acid-containing monomers with acrylates is Carbopol 1342. All Carbopol (trademark) materials are available from Goodrich. Suitable crosspolymers of acrylic acid and acrylate are Pemulen TR1 or Pemulen TR2. Suitable heteropolysaccharide gums are xanthan gum, such as xanthan gum available as Kelzan mu.

[0062] The hair care composition of the present invention may also contain a penetration enhancer.

[0063] According to the present invention, a "penetration enhancer" can be understood as a compound that can penetrate hair follicles and / or scalp to reversibly reduce barrier resistance and increase the bioavailability of active substances to sites of action in hair follicles and / or scalp, such as compounds that increase the bioavailability of active substances to hair bulbs.

[0064] In some embodiments, the penetration enhancer is selected from glycols, such as polyethylene glycol; phospholipids, for example in the form of one or more liposomes; nonionic emulsifiers, such as polysorbate, lauryl ketone, isohexadecane, dodecane, DMSO, ethanol, decanol, isosorbide dimethyl ether and / or combinations thereof.

[0065] In some embodiments, the penetration enhancer is present in an amount of 0.01% to 20% of the total weight of the composition, more preferably at least 0.1% of the weight of the composition, even more preferably at least 1%, even more preferably at least 2%, and most preferably at least 6%. Preferably, the penetration enhancer is present in an amount not exceeding 15% of the weight of the composition, even more preferably not exceeding 10%, even more preferably not exceeding 8%, and most preferably not exceeding 3%.

[0066] In some embodiments, the penetration enhancer is selected from ethanol, isosorbide dimethyl ether, or combinations thereof. Preferably, the penetration enhancer is a combination of isosorbide dimethyl ether and ethanol.

[0067] In some preferred embodiments, the penetration enhancer comprises isosorbide dimethyl ether.

[0068] In one embodiment, isosorbide dimethyl ether is included in an amount of 0.01% to 10% of the total weight of the composition, or in an alternative, in an amount of 0.1% to 5% of the total weight of the composition, or 1% to 3% of the total weight of the composition, such as 2.5% of the total weight of the composition.

[0069] In some implementations, the penetration enhancer comprises ethanol.

[0070] Preferably, ethanol is present in an amount of 0.01% to 20% of the total weight of the composition, more preferably at least 0.1%, even more preferably at least 1%, even more preferably at least 2%, and most preferably at least 3%. Preferably, ethanol is present in an amount not exceeding 15% of the weight of the composition, even more preferably not exceeding 10%, even more preferably not exceeding 8%, and most preferably not exceeding 7%.

[0071] Preferably, the weight ratio of the penetration enhancer to N-acetyltyrosine is 1:0.001 to 1:10, more preferably 1:0.01 to 1:5, and even more preferably 1:0.03 to 1:1. In some embodiments, the weight ratio of the penetration enhancer to N-acetyltyrosine is 1:0.1 to 1:0.5.

[0072] Preferably, the weight ratio of the penetration enhancer to taxonomycin glucoside is from 1:0.00001 to 1:1; more preferably, the weight ratio is from 1:0.00005 to 1:0.1; and even more preferably, from 1:0.0001 to 1:0.01. In some embodiments, the weight ratio of the penetration enhancer to taxonomycin glucoside is from 1:0.001 to 1:0.005.

[0073] Preferably, the weight ratio of isosorbide dimethyl ether to N-acetyltyrosine is from 1:0.001 to 1:5, more preferably from 1:0.01 to 1:1, and even more preferably from 1:0.03 to 1:0.1. In some embodiments, the weight ratio of isosorbide dimethyl ether to N-acetyltyrosine is from 1:0.05 to 1:0.09.

[0074] Preferably, the weight ratio of isosorbide dimethyl ether to taxonomycin glucoside is from 1:0.00001 to 1:1; more preferably, the weight ratio is from 1:0.00005 to 1:0.1; and even more preferably, from 1:0.0001 to 1:0.01. In some embodiments, the weight ratio of isosorbide dimethyl ether to taxonomycin glucoside is from 1:0.002 to 1:0.006.

[0075] Preferably, the weight ratio of ethanol to N-acetyltyrosine is from 1:0.0001 to 1:5, more preferably from 1:0.001 to 1:1, and even more preferably from 1:0.01 to 1:0.5. In some embodiments, the weight ratio of ethanol to N-acetyltyrosine is from 1:0.02 to 1:0.05.

[0076] Preferably, the weight ratio of ethanol to taxonomycin glucoside is from 1:0.00001 to 1:1; more preferably, the weight ratio is from 1:0.00005 to 1:0.1; and even more preferably, from 1:0.0001 to 1:0.01. In some embodiments, the weight ratio of ethanol to taxonomycin glucoside is from 1:0.001 to 1:0.005.

[0077] In some embodiments, the hair care composition also contains additional antioxidants. For example, the compositions of the present invention may also contain epigallocatechin gallate and / or epigallocatechin gallyl glucoside.

[0078] Epigallocatechin gallate (EGCG, also known as epigallocatechin-3-gallate) is an ester of epigallocatechin and gallic acid, and is a type of catechin. Epigallocatechin gallate can help with hair growth. A suitable method for preparing epigallocatechin gallate α-D-glucoside is described in WO 2007 / 144368.

[0079] The amount of epigallocatechin gallate glucoside can be from 0.00001 to 0.60% by weight, more preferably from 0.00010 to 0.060% by weight, and most preferably from 0.00015 to 0.0045% by weight.

[0080] The sources of epigallocatechin gallate and / or epigallocatechin galloglycoside that may be included in the compositions of the present invention include tea leaf extract. Preferably, the amount of tea leaf extract in the hair care compositions of the present invention may be, for example, 0.0001% to 1% of the total weight of the composition, and more preferably 0.0005% to 0.1% of the total weight of the composition, more preferably 0.0009% to 0.01% of the total weight of the composition, and even more preferably 0.0009% to 0.005% of the total weight of the composition.

[0081] In some embodiments of the invention, the composition comprises peas ( Pisum Sativum Extracts prepared by ).

[0082] The pea liquid extracts that can be used in this invention include extracts obtained from the plant pea. The botanical name of pea is... Pisum Sativum Pea peptides deliver abundant and antioxidant benefits. Additionally, pea liquid extract can have film-forming properties, providing protective benefits and a silky feel to hair and skin. Pea peptides can reduce damage caused by free radicals, promoting scalp and hair follicle health.

[0083] The amount of pea liquid extract in the composition of the present invention may be, for example, 0.001% to 10% of the total weight of the composition, more preferably 0.01% to 5% of the total weight of the composition, more preferably 0.01% to 1% of the total weight of the composition, and most preferably 0.005% to 0.03% of the total weight of the composition.

[0084] In a preferred embodiment, the hair care composition of the present invention comprises glycine. Glycine is an amino acid. Glycine may be used at a concentration of 0.001% to 0.50% of the total weight of the composition, more preferably 0.001% to 0.30% of the total weight of the composition, and most preferably 0.001% to 0.008% of the total weight of the composition.

[0085] In some embodiments of the invention, the composition is contained in one or more cosmetically or pharmaceutically acceptable solvents, such as glycerin, propylene glycol, butylene glycol, ethoxylated or propoxylated diethylene glycol, propanol, or isopropanol, to help dissolve the active ingredient of the composition, such as yeast extract.

[0086] According to another advantageous embodiment of the invention, the yeast extract of the invention is first dissolved in a cosmetic or pharmaceutical carrier (such as liposomes) or adsorbed onto a powdered organic polymer, mineral carrier (such as talc and bentonite) before being included in the hair care composition.

[0087] In some embodiments, the compositions of the present invention further comprise yeast lysates. Preferably, the yeast lysates are fermented yeast lysates. Preferably, the composition comprises lysates produced by yeasts of the genus *Saccharomyces* (Saccharomyces cerevisiae). Saccharomyces Materials obtained from yeast lysate fermentation, such as yeast fermentation lysate filtrate.

[0088] In some embodiments, the amount of yeast fermentation lysate filtrate in the hair care composition of the present invention may be, for example, 0.001% to 10% of the total weight of the composition, more preferably 0.001% to 0.5% of the total weight of the composition, more preferably 0.01% to 0.1% of the total weight of the composition, and most preferably 0.01% to 0.1% of the total weight of the composition, for example, about 0.03% to 0.12% of the total weight of the composition.

[0089] In some embodiments, the compositions of the present invention further comprise additional anti-shedding active substances, such as nettle leaf extract (Urtica dioica). Nettle leaf extract can help promote blood flow in the skin or scalp.

[0090] The amount of nettle leaf extract in the hair care composition of the present invention can be, for example, 0.001% to 10% of the total weight of the composition, more preferably 0.01% to 5% of the total weight of the composition, more preferably 0.1% to 1% of the total weight of the composition, and most preferably 0.1% to 0.5% of the total weight of the composition.

[0091] In some embodiments, the compositions of the present invention further comprise a root extract of *Glycyrrhiza glabra*. *Glycyrrhiza glabra* root extract can beneficially provide some anti-inflammatory and / or scalp-soothing effects. *Glycyrrhiza glabra* L. is the root of licorice (a perennial plant belonging to the legume family), and its main pharmacological components are glycyrrhizin (a saponin component) and various flavonoids, organic acids, amino acids, choline, gums, fructose, and glucose.

[0092] The amount of licorice root extract in the hair care composition of the present invention can be, for example, 0.001% to 10% of the total weight of the composition, more preferably 0.01% to 5% of the total weight of the composition, more preferably 0.1% to 1% of the total weight of the composition, and most preferably 0.1% to 0.5% of the total weight of the composition, for example, about 0.1% to 0.3% of the total weight of the composition.

[0093] In some embodiments, the compositions of the present invention further comprise monocarboxylic acids, dicarboxylic acids, or tricarboxylic acids. Preferably, the compositions of the present invention comprise tricarboxylic acids. Suitable tricarboxylic acids include citric acid, aconitic acid, or mixtures thereof. The most preferred tricarboxylic acid is citric acid.

[0094] Preferably, the tricarboxylic acid is present in the composition in an amount of 0.001% to 20% of the total weight of the composition, for example, in an amount of 0.005% to 5% of the total weight of the composition, more preferably in an amount of 0.01% to 1% of the total weight of the composition, even more preferably in an amount of 0.01% to 0.1% of the total weight of the composition, and most preferably in an amount of 0.02% to 0.1% of the total weight of the composition, for example, in an amount of about 0.02% to 0.05% of the total weight of the composition.

[0095] In some embodiments, the composition comprises an amaranth seed extract. Preferably, the composition comprises an amaranth (Amaranthus audatus) seed extract. The amaranth (Amaranthus caudatus) seed extract contains various small peptides and polysaccharides, including phenylalanine, histidine, and other unique peptides, which can penetrate into the hair fiber and attach to the internal structure, thereby improving hair properties.

[0096] Preferably, the amaranth seed extract is present in the composition in an amount of 0.001% to 10% of the total weight of the composition, for example, 0.005% to 5% of the total weight of the composition, more preferably 0.01% to 1% of the total weight of the composition, more preferably 0.05% to 0.5% of the total weight of the composition, and most preferably 0.08% to 0.2% of the total weight of the composition.

[0097] In some embodiments, the composition comprises grape extract. Preferably, the grape extract is present in the composition in an amount of 0.000001% to 0.001% by weight of the total composition, for example, in an amount of 0.000005% to 0.00005% by weight of the total composition.

[0098] In some embodiments, the hair care composition comprises a first active component and a second active component. The first and second active components may optionally be blended with further ingredients and components to form the hair care composition of the present invention.

[0099] In some embodiments, the first active component comprises: (i) Taxonitrile glucoside; and (ii) N-acetyltyrosine.

[0100] The amount of taurine glucoside in the first active component may be, for example, 0.01% to 0.50% of the total weight of the first active component, more preferably 0.05% to 0.25% of the total weight of the first active component, and most preferably 0.07% to 0.23% of the total weight of the first active component.

[0101] The ratio of taxanein glucoside to taxanein in the first active component can be as described above for the total hair care composition.

[0102] In some embodiments, the first active component, taxonomyl glucoside and / or taxonomyl, may be derived from European larch. For example, taxonomyl glucoside and / or taxonomyl may be included in European larch extract.

[0103] The amount of European larch extract in the first active component may be, for example, 0.01% to 0.1% of the total weight of the first active component, more preferably 0.05% to 0.8% of the total weight of the first active component, and most preferably 0.1% to 0.7% of the total weight of the first active component.

[0104] The amount of N-acetyltyrosine in the hair care active agent of the present invention can be, for example, 1.0% to 30% of the total weight of the first active component, more preferably 10% to 20% of the total weight of the first active component, and most preferably 15% to 20% of the total weight of the first active component.

[0105] The first active ingredient may include glycine. For example, glycine may be used in an amount of 0.01% to 1% of the total weight of the first active ingredient, more preferably 0.10% to 0.80% of the total weight of the first active ingredient, and most preferably 0.20% to 0.60% of the total weight of the first active ingredient.

[0106] The first active ingredient may include tea leaf extract. For example, the tea leaf extract may be included in an amount of 0.01% to 1.5% of the total weight of the first active ingredient, more preferably 0.05% to 1% of the total weight of the first active ingredient, and most preferably 0.08% to 0.5% of the total weight of the first active ingredient.

[0107] The first active ingredient may include epigallocatechin gallate glucoside. For example, epigallocatechin gallate glucoside may be included in an amount of 0.001% to 0.60% of the total weight of the first active ingredient, more preferably 0.010% to 0.060% of the total weight of the first active ingredient, and most preferably 0.015% to 0.045% of the total weight of the first active ingredient.

[0108] The first active component may include a preservative, such as sodium metabisulfite. For example, the preservative may be included in an amount of 0.01% to 1.00% of the total weight of the first active component, more preferably 0.10% to 0.750% of the total weight of the first active component, and most preferably 0.45% to 0.55% of the total weight of the first active component.

[0109] In some embodiments, the first active component may comprise a zinc salt, preferably zinc chloride. For example, the zinc salt may be included in an amount of 0.01% to 1% of the total weight of the first active component, more preferably 0.05% to 0.5% of the total weight of the first active component, and most preferably 0.08% to 0.2% of the total weight of the first active component.

[0110] The first active ingredient may contain one or more cosmetically or pharmaceutically acceptable solvents, such as glycerin, propylene glycol, butylene glycol, ethoxylated or propoxylated diethylene glycol, ethanol, propanol, or isopropanol, to help dissolve the active ingredient of the first active ingredient.

[0111] For example, glycerin may be included in an amount of 1% to 95% of the total weight of the first active component, more preferably 10% to 80% of the total weight of the first active component, and most preferably 20% to 60% of the total weight of the first active component.

[0112] The first active ingredient may be present in the hair care composition in an amount of 0.001% to 20% of the total weight of the composition, for example, in an amount of 0.005% to 10% of the total weight of the composition, more preferably in an amount of 0.01% to 5% of the total weight of the composition, more preferably in an amount of 0.1% to 3% of the total weight of the composition, and most preferably in an amount of 0.5% to 2% of the total weight of the composition.

[0113] In some embodiments, the first active component may comprise a composition as described in WO2020089216.

[0114] The first active component can be provided, for example, as Darkenyl™, a commercially available material from Givaudan.

[0115] In some embodiments, the second active component comprises: (iii) At least one yeast peptide hydrolysate of a Saccharomyces cerevisiae species.

[0116] Preferably, the peptide hydrolysate is a low molecular weight peptide hydrolysate. Preferably, the peptide hydrolysate has a molecular weight of 6 kDa or less, more preferably 5 kDa or less, even more preferably 3 kDa or less, such as 1 kDa or less, and most preferably has a molecular weight of less than 600 Da.

[0117] The peptide hydrolysis products obtained according to the present invention can be analyzed using high-performance liquid chromatography (HPLC) with appropriate solvent systems / gradients known in the art.

[0118] Preferably, the yeast peptide hydrolysate of the second active component is prepared by hydrolyzing yeast proteins from the Saccharomyces cerevisiae species. Preferably, the yeast peptide hydrolysate of the second active component is an aqueous extract. The extract disclosed herein is preferably a purified aqueous extract.

[0119] The yeast peptide hydrolysate may be contained in an amount of 0.01% to 1.00% of the total weight of the second active component, more preferably 0.10% to 0.80% of the total weight of the second active component, and most preferably 0.30% to 0.60% of the total weight of the second active component.

[0120] The second active component may include a preservative, such as sodium benzoate, potassium sorbate, or combinations thereof. For example, the preservative may be included in an amount of 0.01% to 1.00% of the total weight of the second active component, more preferably 0.10% to 0.80% of the total weight of the second active component, and most preferably 0.30% to 0.60% of the total weight of the second active component, such as about 0.45% of the total weight of the second active component.

[0121] The second active ingredient may contain one or more cosmetically or pharmaceutically acceptable solvents, such as glycerin, propylene glycol, butylene glycol, ethoxylated or propoxylated diethylene glycol, ethanol, propanol, or isopropanol, to help dissolve the active ingredient of the first active ingredient.

[0122] For example, glycerol may be included in an amount of 1% to 95% of the total weight of the second active component, more preferably 10% to 80% of the total weight of the second active component, and most preferably 20% to 60% of the total weight of the second active component.

[0123] The second active ingredient may be present in the hair care composition in an amount of 0.001% to 20% of the total weight of the composition, for example, in an amount of 0.005% to 10% of the total weight of the composition, more preferably in an amount of 0.01% to 5% of the total weight of the composition, more preferably in an amount of 0.1% to 3% of the total weight of the composition, and most preferably in an amount of 0.5% to 2% of the total weight of the composition.

[0124] The second active component can be provided, for example, as Dynagen™, a commercially available material from Ashland.

[0125] In some embodiments, the hair care composition comprises a third active ingredient, which comprises zinc sulfate. In some embodiments, the amount of zinc sulfate in the third active ingredient may be, for example, 0.00001% to 5% of the total weight of the third active ingredient, preferably 0.0001% to 3% of the total weight of the third active ingredient, more preferably 0.001% to 1% of the total weight of the third active ingredient, more preferably 0.01% to 0.1% of the total weight of the third active ingredient, and most preferably at a concentration of 0.01% to 0.05% of the total weight of the third active ingredient, for example, at most 0.03%.

[0126] In some embodiments, the hair care composition comprises a fourth active ingredient. In some embodiments, the fourth active ingredient may comprise one or more of nettle leaf extract, licorice root extract, Querus robur bark extract, tricarboxylic acid, and yeast fermentation lysate filtrate.

[0127] The nettle leaf extract may be included in the fourth active component in an amount of 0.1% to 30% of the total weight of the fourth active component, more preferably 1% to 20% of the total weight of the fourth active component, and most preferably 2% to 15% of the total weight of the fourth active component.

[0128] The licorice root extract may be included in the fourth active component in an amount of 0.1% to 20% of the total weight of the fourth active component, more preferably 1% to 10% of the total weight of the fourth active component, and most preferably 2% to 8% of the total weight of the fourth active component.

[0129] The oak bark extract may be included in the fourth active component in an amount of 0.1% to 20% of the total weight of the fourth active component, more preferably 1% to 10% of the total weight of the fourth active component, and most preferably 2% to 8% of the total weight of the fourth active component.

[0130] Tricarboxylic acid may be included in the fourth active component in an amount of 0.01% to 5% of the total weight of the fourth active component, more preferably 0.1% to 3% of the total weight of the fourth active component, and most preferably 1% to 2% of the total weight of the fourth active component.

[0131] The yeast fermentation lysate filtrate may be included in the fourth active component in an amount of 0.1% to 20% of the total weight of the fourth active component, more preferably 1% to 10% of the total weight of the fourth active component, and most preferably 2% to 5% of the total weight of the fourth active component.

[0132] The fourth active component may include a preservative, such as sodium benzoate, potassium sorbate, or combinations thereof. For example, the preservative may be included in an amount of 0.01% to 1% of the total weight of the fourth active component, more preferably 0.1% to 0.8% of the total weight of the fourth active component, and most preferably 0.2% to 0.6% of the total weight of the fourth active component.

[0133] The fourth active ingredient may be present in the hair care composition in an amount of 0.001% to 20% of the total weight of the composition, for example, in an amount of 0.005% to 10% of the total weight of the composition, more preferably in an amount of 0.01% to 8% of the total weight of the composition, more preferably in an amount of 0.1% to 6% of the total weight of the composition, and most preferably in an amount of 0.5% to 5% of the total weight of the composition.

[0134] The hair care composition of the present invention is primarily intended for topical application to the hair and / or scalp of human subjects to improve hair properties.

[0135] Preferably, the hair care composition is selected from shampoo-free hair compositions, hair masks, leave-in hair care compositions, and pre-treatment hair care compositions, and most preferably from shampoo-free hair compositions, hair masks, and leave-in compositions. The pre-treatment composition is preferably a leave-in hair care composition. The hair care composition of the present invention may also be a "shampoo-free" composition, which is applied to the hair and then partially rinsed away.

[0136] The wash-off compositions used in this invention are typically left on wet hair for 1 to 2 minutes before being washed off. Typically, about 1 g to about 50 g of the composition is applied to the hair or scalp.

[0137] The hair mask used in this invention is typically left on the hair for 3 to 10 minutes, preferably 3 to 5 minutes, more preferably 4 to 5 minutes, before rinsing it off.

[0138] The leave-in compositions used in this invention are typically applied to the hair and left on the hair for more than 10 minutes, and preferably applied to the hair after washing and not rinsed off until the next wash.

[0139] Based on total weight, the hair care composition of the present invention typically contains about 20% to about 95%, preferably at least 30%, more preferably at least 40%, even more preferably at least 50%, and even more preferably at least 60% or even at least 70%, but generally not exceeding 94%, preferably not exceeding 93%, more preferably not exceeding 92%, even more preferably not exceeding 91%, and even more preferably not exceeding 90% or even not exceeding 80% by weight of water. Other organic solvents, such as lower alkyl alcohols and polyols, may also be present. Examples of lower alkyl alcohols include C1 to C6 monohydric alcohols, such as ethanol and isopropanol. Examples of polyols include propylene glycol, hexanediol, glycerol, and propylene glycol. Mixtures of any of the above-mentioned organic solvents may also be used.

[0140] Uses and methods In a further aspect, the present invention relates to the non-therapeutic use of the hair care compositions of the present invention.

[0141] In some embodiments, the present invention relates to the non-therapeutic use of the hair care compositions of the present invention for reducing the appearance of gray or white hair in an individual, for reducing age-related functional changes in hair pigmentation, for increasing melanin production in gray hair, for increasing cell proliferation, for increasing melanocyte proliferation, and for increasing the level and / or activity of tyrosinase.

[0142] In some embodiments, the present invention relates to the non-therapeutic use of the hair care compositions of the present invention for reducing age-related functional changes in hair pigmentation, for improving hair volume, for increasing hair thickness, for increasing hair count, for improving hair strength, for reducing hair loss, and / or for hair fiber repair.

[0143] In some embodiments, the present invention relates to the non-therapeutic uses of the hair care compositions of the present invention for reducing age-related functional changes in hair pigmentation and for improving scalp health, reducing scalp inflammation, reducing oxidative stress on the scalp, reducing oxidized lipid substances on the scalp, improving sebum control, and / or improving the scalp barrier.

[0144] In some embodiments, the present invention relates to the non-therapeutic use of the hair care compositions of the present invention for reducing oxidative stress in melanocytes, preferably in hair follicles and / or scalp.

[0145] In some embodiments, the present invention relates to hair care compositions for reducing the appearance of gray or white hair in an individual, for reducing age-related functional changes in hair pigmentation, for increasing melanin production in gray hair, for increasing cell proliferation, for increasing melanocyte proliferation, and for increasing the level and / or activity of tyrosinase.

[0146] In some embodiments, the present invention relates to hair care compositions for reducing age-related functional changes in hair pigmentation and for improving hair volume, increasing hair thickness, increasing hair count, improving hair strength, reducing hair loss, and / or for fiber repair.

[0147] In some embodiments, the present invention relates to hair care compositions for reducing age-related functional changes in hair pigmentation and for improving scalp health, reducing scalp inflammation, reducing oxidative stress on the scalp, reducing oxidized lipid substances on the scalp, improving sebum control, and / or improving the scalp barrier.

[0148] In a further aspect, the present invention relates to a treatment method comprising the hair care composition of the present invention.

[0149] In some embodiments, the present invention relates to a method for reducing the presence of gray or white hair in an individual, reducing age-related functional changes in hair pigmentation, increasing melanin production in gray hair, increasing cell proliferation, increasing melanocyte proliferation, and increasing the level and / or activity of tyrosinase; said method comprising applying the hair care composition of the present invention to hair or scalp.

[0150] In some embodiments, the present invention relates to methods for reducing age-related functional changes in hair pigmentation, and to methods for improving hair volume, increasing hair thickness, increasing hair count, increasing hair strength, reducing hair loss, and / or providing fiber repair; said methods include applying the hair care composition of the present invention to the hair or scalp.

[0151] In some embodiments, the present invention relates to a method for reducing age-related functional changes in hair pigmentation, and to a method for improving scalp health, reducing scalp inflammation, reducing oxidative stress on the scalp, reducing oxidized lipid substances on the scalp, improving sebum control, and / or improving the scalp barrier; said method comprising applying the hair care composition of the present invention to the hair or scalp.

[0152] In some embodiments, the present invention relates to a method for reducing oxidative stress in melanocytes, the method comprising applying the hair care composition of the invention to hair or scalp. Preferably, melanocytes in hair follicles and / or scalp.

[0153] The features disclosed in the foregoing specification or the appended claims may be used alone or in any combination of these features to implement the invention, when appropriate.

[0154] While the present invention has been described in conjunction with the foregoing exemplary embodiments, many equivalent modifications and variations will be apparent to those skilled in the art when this disclosure is given. Therefore, the exemplary embodiments of the invention described above are considered illustrative rather than restrictive. Various changes may be made to the described embodiments without departing from the spirit and scope of the invention.

[0155] To avoid any ambiguity, any theoretical explanations provided herein are intended to enhance the reader's understanding. The inventor does not wish to be bound by any of these theoretical explanations. Any section headings used herein are for organizational purposes only and should not be construed as limiting the subject matter described.

[0156] Throughout this specification, including the following claims, unless the context otherwise requires, the words “comprising” and “including”, as well as variations such as “containing,” “comprising,” and “including”, shall be understood to imply inclusion of the said integer or step or group of integers or steps, but do not exclude any other integer or step or group of integers or steps.

[0157] It should be noted that, as used in the specification and appended claims, the singular forms “a,” “an,” and “the” include plural indicators unless the context clearly specifies otherwise. A range may be expressed herein as from “about” a particular value and / or to “about” another particular value. When expressing such a range, another embodiment includes from one particular value and / or to another particular value. Similarly, when a value is expressed as an approximation using the antecedent “about,” it should be understood that the particular value forms another embodiment. The term “about” with respect to numerical values ​​is optional and means, for example, + / - 10%.

[0158] The invention will now be further described with reference to the following embodiments. Unless otherwise stated, all percentages are weight percentages based on total weight.

[0159] Example Example 1: Hair care composition - preparation method Fill the container with deionized water. Separately disperse the powdered rheology modifier and add it to the container to ensure proper hydration. Heat the container to 60°C, then add the preservative until dissolved. Cool the container to room temperature. Once 35°C or lower is reached, add zinc sulfate, Dynagen™, and Darkenyl™ while continuously mixing, ensuring thorough mixing between each addition. Add the remaining ingredients and mix. Then adjust the pH of the solution to 4.5–5.0 using an 18% sodium hydroxide aqueous solution.

[0160] Example 2: Hair care composition Table 1

[0161] Example 3: Effects of hair care composition on hair pigmentation and other hair characteristics In this embodiment, a single-center, single-blind, home-use clinical study was conducted over a period of 3 months in healthy male and female subjects to assess the efficacy of the scalp treatment through photography, expert visual evaluation, and self-perception questionnaires.

[0162] The study included 32 eligible participants. 30 completed the study. Two participants did not complete the study and withdrew for personal reasons.

[0163] Provide the hair care compositions shown in Table 1, along with instructions for use. During the study period (preferred, at night or during the day), instruct subjects to apply 2–3 droppers of the product directly (massaging) to the dry or damp scalp once daily without rinsing.

[0164] Research Procedures Day 1, Visit 1 (baseline) Upon arrival at the research center, participants had clean, unstyled hair, were free of products, and had clean faces without makeup for photographic purposes. On Day 1, expert stylists graded, evaluated, and photographed them. Participants were then given test products for home use, along with instructions and a weekly diary.

[0165] Month 1, 2 visits Subjects arriving at the research center had clean, unstyled hair, were product-free, and had clean faces without makeup for photographic purposes. In the first month, expert stylists graded, evaluated, and photographed the subjects. Subject diary compliance and any adverse reactions were checked.

[0166] In the second month, 3 visits were made. Subjects arriving at the research center had clean, unstyled hair, were product-free, and had clean faces without makeup for photographic purposes. In the second month, expert stylists graded, evaluated, and photographed the subjects. Subject diary compliance and any adverse reactions were checked.

[0167] Month 3, Visit 4 (Study concluded) Subjects arriving at the research center had clean, unstyled hair, were product-free, and had clean faces without makeup for photographic purposes. In the third month, expert stylists graded, evaluated, and photographed the subjects. Subject diary compliance and any adverse reactions were checked.

[0168] result Professional photography images The following angles were used to photograph the subjects at baseline, month 1, month 2, and month 3 (at the end of the study): • Front view - looking straight ahead, with the nose pointing towards the camera. • Left view - An angled left view with the left sideburn facing the camera. • Right view - An angled right view with the right sideburn facing the camera. hair volume Visual inspection and grading assessment Expert visual grading was used to determine hair fullness at baseline and after 1, 2, and 3 months of home use of the test product. Professional graders visually examined the hair at a distance of approximately 30 cm from the area using a 60-watt pearlescent light bulb, using the following grading scale: 0- Dense and abundant 1- Abundant abundance 2 - Greater than average abundance 3-Average Abundance 4- Soft 5- Very soft Table 2

[0169] [1] Percentage change is calculated from the average change relative to the baseline. [2] Percentage change was calculated individually for each subject and averaged (note that if it was 0 at baseline, it was not calculated for that subject).

[0170] Wilcoxon signed-rank test within the treatment showed that the test product was statistically significant in improving hair fullness (p<0.05). The baseline mean of 4.30 was observed to improve to 3.55 after 1 month of home use, to 2.92 after 2 months of home use, and to 2.77 after 3 months of home use.

[0171] Compared to baseline, the mean percentage change after 3 months of test product use showed an improvement from 0.00% (post-baseline application) to -17.44% at 1 month, -32.17% at 2 months, and -35.66% at the end of the study.

[0172] After 2 months of home use, 100% of participants showed a visible improvement in hair volume. After 3 months of home use, 100% of participants continued to show improvement.

[0173] Hair thickness Visual inspection and grading assessment Expert visual grading was used to determine baseline hair thickness and then after 1, 2, and 3 months of home use of the test product. Professional graders visually examined the hair at a distance of approximately 30 cm from the site using a 60-watt pearlescent light bulb, using the following grading scale: 0-Very thick 1-thick 2-average 3-fine 4-thin 5 - Very thin and fragile Table 3

[0174] [1] Percentage change is calculated from the average change relative to the baseline. [2] Percentage change calculated individually for each subject and averaged (note that if it was 0 at baseline, it was not calculated for that subject).

[0175] Wilcoxon signed-rank test within the treatment showed that the test product was statistically significant in increasing hair thickness (p<0.0). An improvement was observed in the baseline mean of 4.03 after 1 month of home use, to 3.33, to 2.77 after 2 months, and to a mean of 2.75 after 3 months of home use. Compared to baseline, the mean percentage change after 3 months of test product use showed an improvement from 0.00% (post-baseline application) to -17.36% at 1 month, to -31.40% at 2 months, and to -31.82% at the end of the study.

[0176] After two months of home use, 100% of the subjects showed a visible improvement in hair thickness, which gradually decreased to 93.33% by the third month.

[0177] Hair count Macro hair count At the baseline visit, the assessor designated a 2cm × 2cm area where sparse hair was clearly visible. This area served as a site for macroscopic grading, from which the assessor evaluated hair count (density) and gray hair count. The designated area was recorded and used to locate the site during subsequent visits. These assessments were performed at baseline, 1 month, 2 months, and 3 months (at the end of the study).

[0178] Table 4

[0179] [1] Percentage change is calculated from the average change relative to the baseline. [2] Percentage change calculated individually for each subject and averaged (note that if it was 0 at baseline, it was not calculated for that subject).

[0180] Wilcoxon signed-rank test within the treatment showed that the test product was statistically significant in increasing hair count (p<0.05). The baseline mean of 31.97 improved to 35.30 after 1 month of home use, 38.87 after 2 months, and 39.70 after 3 months. Compared to baseline, the mean percentage change after 3 months of test product use showed improvement from 0.00% (post-baseline application) to 10.43% at 1 month, 21.58% at 2 months, and 24.19% at the end of the study.

[0181] 100% of the subjects showed a visible increase in hair count after 2 months of home use, and 100% of the subjects continued to show an increase in hair count after 3 months of home use.

[0182] Gray hair count Macroscopic grading was used to monitor gray hair counts at baseline and at 1, 2, and 3 months after home use of the test product. At the baseline visit, gray hair was present in 21 of the 30 participants. The assessor designated a 2cm × 2cm area where sparse and gray hairs were clearly visible. This area was used as the site for macroscopic grading, from which the assessor evaluated the gray hair count. The designated area was recorded and used to locate the site during subsequent visits.

[0183] Table 5

[0184] [1] Percentage change is calculated from the average change relative to the baseline. [2] Percentage change calculated individually for each subject and averaged (note that if it is 0 at baseline, it is not calculated for that subject).

[0185] Wilcoxon signed-rank test within the treatment showed that the test product was statistically significant in reducing gray hair count (p<0.05). The baseline mean of 7.76 improved to 6.67 after 1 month of home use, 6.10 after 2 months, and 5.86 after 3 months. Compared to baseline, the mean percentage change after 3 months of test product use showed improvement from 0.00% (post-baseline application) to -12.88% at 1 month, -21.47% at 2 months, and -24.54% by the end of the study.

[0186] 76.19% of the subjects observed a reduction in gray hair count after 2 and 3 months of home use.

[0187] Brush friction count Brush Friction Counting Method (BFCM) BFCM involves brushing the subject's hair for 10 seconds at a rate of 1 stroke / second with moderate force using a large paddle brush (e.g., Kent Airhedz Mega Taming Brush) with a padded head and narrow barbs with rounded tips. The entire head is brushed. The hair caught by the brush is then removed. Broken / damaged hairs are separated from intact hairs and examined under a magnifying glass at 2× magnification. The number of broken / damaged hairs and intact hairs is counted. These assessments are performed at baseline, 1 month, 2 months, and 3 months (at the end of the study).

[0188] Complete brush friction count Table 6

[0189] [1] Percentage change is calculated from the average change relative to the baseline. [2] Percentage change calculated individually for each subject and averaged (note that if it was 0 at baseline, it was not calculated for that subject).

[0190] Brush friction count damaged Table 7

[0191] [1] Percentage change is calculated from the average change relative to the baseline. [2] Percentage change calculated individually for each subject and averaged (note that if it was 0 at baseline, it was not calculated for that subject).

[0192] Statistical analysis using the internal t-test revealed a statistically significant improvement in the count of broken / damaged hairs, intact hairs, and total hair count.

[0193] The total mean of intact hairs counted at baseline was recorded as 7.80, and after 3 months of home use, the total mean was observed to be 2.30, representing a percentage improvement of -70.51%.

[0194] The total mean number of broken hairs counted at baseline was recorded as 6.67, and after 3 months of home use, the total mean number was recorded as 1.03, representing a percentage improvement of -84.50%.

[0195] Example 4: Hair care shampoo Fill the container with deionized water. Separately disperse the carbomer and add it to the container, ensuring proper hydration. Heat the container to 60°C, then add the preservative, including pentylene glycol, until dissolved. Cool the container to room temperature. Once 35°C or lower is reached, add zinc sulfate, Dynagen™, and Darkenyl™ while continuously mixing, mixing thoroughly between additions. Add the remaining ingredients and mix. Then adjust the pH of the solution to 4.5–5.0 as needed using sodium hydroxide and / or citric acid solution.

[0196] Table 8

[0197] Example 5: Determination of dichlorofluorescein diacetate (DCFDA) Adult primary human epidermal melanocytes were cultured in T75 flasks in melanocyte culture medium supplemented with a mixture of melanocyte growth factors and antibiotics, and the T75 flasks were maintained at 37°C in a humidified chamber with 5% CO2. Cells were then transferred in microtiter plates at approximately 5 × 10⁻⁶ cells / mL.4 Cells were further passaged at the desired density in a humidified chamber at 37°C with 5% CO2 for 24 hours, as described above. After 24 hours, cells were treated with the desired concentration of test material or a combination of test materials and 5 μM menadione, and further incubated for 6 hours as described above. Following incubation, ROS levels were assessed by incubating cells in DCFDA dye for 45 minutes. Cells were then washed, and fluorescence was measured at excitation: 485 nm and emission: 535 nm. Experiments were performed in duplicate (n=2) on three different donor cells (n=3). Data are expressed as % ROS levels compared to menadione.

[0198] Concentration of test material in cell culture medium : Darkenyl™ (available from Givaudan) - 0.005% containing taurine glucoside / acetyltyrosine Dynagen™ yeast peptide hydrolysate from the Saccharomyces cerevisiae species (available from Ashland) - 0.75% Statistical analysis For each test, the average of two replicates from three different experiments was used to show the percentage difference compared to the menadione-treated control. A t-test was performed to assess statistical significance for the comparison between the menadione control and the corresponding treatment.

[0199] Table 9

[0200] Menadione induces oxidative stress in melanocytes by generating reactive oxygen species (ROS). The antioxidant effect of the test material on menadione-stressed melanocytes was evaluated by comparing ROS levels in treated and untreated stressed cells. Compared to ROS levels in menadione-stressed cells, yeast peptide hydrolysates from the *Saccharomyces cerevisiae* species reduced ROS levels. This indicates that the inclusion of yeast peptide hydrolysates from *Saccharomyces cerevisiae* species in the formulation improves the antioxidant activity of melanocytes. Furthermore, the inclusion of yeast peptide hydrolysates from *Saccharomyces cerevisiae* species in the composition improved the antioxidant properties of the taurine glucoside / acetyltyrosine mixture in the assay.

Claims

1. A hair care composition comprising: (i) Taxonitrile glucoside; (ii) N-acetyltyrosine; (iii) at least one yeast peptide hydrolysate of a *Saccharomyces cerevisiae* species; and (iv) At least one zinc salt.

2. The hair care composition according to claim 1, wherein the zinc salt is selected from zinc chloride, zinc sulfate, zinc gluconate, zinc pyrithione, or a combination thereof, preferably zinc sulfate.

3. The hair care composition according to any one of the preceding claims, wherein the amount of zinc salt in the hair care composition is 0.00001% to 5% of the total weight of the composition, preferably 0.0001% to 3% of the total weight of the composition, more preferably 0.001% to 1% of the total weight of the composition, more preferably 0.01% to 0.1% of the total weight of the composition, more preferably 0.01% to 0.05% of the total weight of the composition, and most preferably 0.01% to 0.03% of the total weight of the composition.

4. The hair care composition according to any one of the preceding claims, wherein the composition further comprises taurine.

5. The hair care composition according to any one of the preceding claims, wherein the amount of taurine glucoside in the hair care composition is 0.0001% to 0.50% of the total weight of the composition, more preferably 0.0005% to 0.25% of the total weight of the composition, even more preferably 0.0007% to 0.009% of the total weight of the composition, preferably 0.0009% to 0.007% of the total weight of the composition, and most preferably 0.001% to 0.004% of the total weight of the composition.

6. The hair care composition according to any one of the preceding claims, wherein the amount of N-acetyltyrosine in the hair care composition is from 0.001% to 30% of the total weight of the composition, preferably from 0.05% to 20% of the total weight of the composition, more preferably from 0.1% to 5% of the total weight of the composition, and even more preferably from 0.15% to 0.9% of the total weight of the composition, and most preferably from 0.15% to 0.4% of the total weight of the composition.

7. The hair care composition according to any one of the preceding claims, wherein the weight ratio of taurine glucoside to N-acetyltyrosine is preferably 1:500 to 1:1, more preferably 1:300 to 1:50, and most preferably 1:200 to 1:100, for example about 1:

150.

8. The hair care composition according to any one of the preceding claims, wherein the amount of yeast peptide hydrolysate of the Saccharomyces species in the hair composition is from 0.0001% to 20% of the total weight of the composition, preferably from 0.001% to 5% of the total weight of the composition, and more preferably from 0.001% to 0.01% of the total weight of the composition.

9. The hair care composition according to any one of the preceding claims, wherein the weight ratio of the yeast peptide hydrolysate of the Saccharomyces cerevisiae species to the zinc salt is 1:0.01 to 1:10, more preferably 1:0.1 to 1:5, even more preferably 1:0.15 to 1:3, and most preferably 1:1 to 1:2.

5.

10. The hair care composition according to any one of the preceding claims, wherein the weight ratio of the yeast peptide hydrolysate of the Saccharomyces cerevisiae species to N-acetyltyrosine is 1:0.5 to 1:100, more preferably 1:1 to 1:50, even more preferably 1:1:5 to 1:30, and most preferably 1:10 to 1:

25.

11. The hair care composition according to any one of the preceding claims, wherein the weight ratio of the yeast peptide hydrolysate of the Saccharomyces cerevisiae species to taurine glucoside is 1:0.001 to 1:5, more preferably 1:0.01 to 1:3, more preferably 1:0.05 to 1:1, and most preferably 1:0.1 to 1:0.

5.

12. The hair care composition according to any one of the preceding claims for non-therapeutic uses for reducing age-related functional changes in hair pigmentation, increasing melanin production in gray hair, reducing oxidative stress on the scalp, and reducing oxidized lipid substances on the scalp.

13. The hair care composition according to any one of claims 1 to 11 for non-therapeutic use in reducing oxidative stress in melanocytes.

14. A method for reducing age-related functional changes in hair pigmentation, increasing melanin production in gray hair, reducing oxidative stress on the scalp, and reducing oxidized lipid substances on the scalp, the method comprising applying a hair care composition according to any one of claims 1-11 to the hair or scalp.

15. A method for reducing oxidative stress in melanocytes, the method comprising applying a hair care composition according to any one of claims 1-11 to hair or scalp.

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