Modified bioluminescent enzymes

By developing novel bioluminescent enzymes and corresponding nucleic acid expression systems, the problems of insufficient photon yield and toxicity in existing bioluminescent pathways in plants, fungi, and mammalian hosts have been solved, enabling the application of autonomous bioluminescence and labeling tools.

CN122396765APending Publication Date: 2026-07-14NENGGUANG BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NENGGUANG BIOTECHNOLOGY CO LTD
Filing Date
2024-08-28
Publication Date
2026-07-14

AI Technical Summary

Technical Problem

Existing bioluminescence pathways have limited applications in plant, fungal, and mammalian hosts due to insufficient photon yield and toxicity issues, failing to meet the technical requirements of certain applications.

Method used

Develop novel bioluminescent enzymes, particularly trichoscin hydroxylase and luciferase and their functional fragments, to catalyze the conversion of trichoscin to 3-hydroxytrichoscin and its further oxidation to emit light, and combine them with nucleic acids encoding these enzymes and expression systems to achieve autonomous bioluminescence.

Benefits of technology

Autonomous bioluminescence in transgenic organisms was achieved without relying on exogenous luciferin, improving photon yield and overcoming toxicity issues, making it suitable as a labeling and reporting tool for multicellular organisms.

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Abstract

Provided herein are one or more novel variants of luciferase and fungal luciferin biosynthetic enzymes, nucleic acids capable of encoding these enzymes, and proteins capable of catalyzing certain stages of fungal luciferin biosynthesis. More specifically, one or more bioluminescent enzymes, in particular one or more phleomycin hydroxylases and luciferases, and functional fragments thereof, and nucleic acids encoding the same are disclosed.
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