水培条件下丛枝菌根真菌快速有效定殖茶树根系的方法

By using host plants such as white clover to co-plant tea trees under hydroponic conditions, the complex and unstable colonization of arbuscular mycorrhizal fungi in tea tree roots was solved, achieving rapid and effective colonization and improving the nutrient absorption efficiency of tea trees.

CN122397607APending Publication Date: 2026-07-17HENAN AGRICULTURAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN AGRICULTURAL UNIVERSITY
Filing Date
2026-06-18
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing methods for colonizing arbuscular mycorrhizal fungi under hydroponic conditions are complex to operate, difficult to observe, and have unstable colonization results, making it difficult to meet actual production needs.

Method used

Under hydroponic conditions, host plants such as white clover are co-planted with tea trees. Through root cross-fertilization and the hydroponic environment, arbuscular mycorrhizal fungi (AMF) are colonized into the tea tree roots. White clover is used as a bridge to achieve stable supply and propagation of AMF. This is combined with intercropping and co-cultivation using phosphorus-deficient nutrient solution and tea tree nutrient solution.

Benefits of technology

It enables rapid and effective colonization of arbuscular mycorrhizal fungi in tea tree roots, improves nitrogen absorption and utilization efficiency, provides stable colonization results, simplifies the operation process, and reduces costs.

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Abstract

本发明提供了一种水培条件下丛枝菌根真菌快速有效定殖茶树根系的方法,该方法以白三叶草、苜蓿或紫云英为寄主植物,采用河砂、蛭石和草炭复合基质盆栽扩繁丛枝菌根真菌(AMF),经种子消毒、定点接种菌剂、缺磷营养液胁迫培养,获得根系富含AMF泡囊与孢子的寄主植物;再将根系带AMF的寄主植物与半年生茶苗在1 / 4茶树营养液体系中水培间作共培养2‑3个月,依托寄主植物介导作用实现AMF在茶树根系的高效定殖。本发明方法解决了土培操作复杂,观测困难的问题,同时解决了单纯水培定殖效果差,孢子繁殖不稳定等问题,且操作方便,具有良好的应用前景。
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