一种木地肤简易无糖组织培养方法

By optimizing the disinfection process and sugar-free culture technology for Kochia scoparia seeds, and combining it with a special substrate and CO2 to replace sucrose, the problems of low propagation efficiency and substrate incompatibility of Kochia scoparia were solved. This enabled efficient and sterile tissue culture seedling production, improved seedling quality and survival rate, and met the needs of large-scale production.

CN122397621APending Publication Date: 2026-07-17CHIFENG UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHIFENG UNIV
Filing Date
2026-04-10
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Kochia scoparia suffers from low propagation efficiency, high contamination rate in traditional sugar tissue culture, and stem rot and poor rooting due to unsuitable substrates, making it difficult to meet the needs of large-scale production.

Method used

Using Kochia scoparia seeds as explants, and by optimizing the disinfection process and sugar-free culture technology, combined with a special substrate of vermiculite, perlite and peat moss, CO2 was used to replace sucrose as a carbon source, and the culture environment parameters were optimized to achieve efficient proliferation and rooting of sterile seedlings.

Benefits of technology

It has enabled the efficient and pollution-free production of high-quality tissue culture seedlings, improved seedling quality and transplant survival rate, and met the needs of large-scale production.

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Abstract

本发明公开了一种木地肤简易无糖组织培养方法,属于植物组织培养技术领域。该方法以木地肤种子为外植体,依次经消毒、诱导培养、无糖增殖培养和炼苗移栽步骤;其中无糖增殖培养采用由蛭石、珍珠岩和草炭土按体积比3:1:2混合的基质,以及不含蔗糖的液体培养基,并在通入CO2的培养环境下进行光照培养。本发明通过优化消毒流程、适配藜科植物特性的基质和无糖培养技术,显著降低了污染率,提高了种子萌发率、生根率和移栽成活率,培养周期短,种苗健壮,实现了木地肤优质种苗的高效、规模化生产,适用于木地肤种苗繁育及生态修复。
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