桐花树无菌芽生根方法

By disinfecting the hypocotyl of the tung tree and using an optimized rooting medium, the problem of difficult rooting of aseptic buds in tung trees was solved, achieving efficient rooting and the cultivation of superior seedlings, thus breaking through the bottleneck of tung tree tissue culture technology.

CN122397626APending Publication Date: 2026-07-17ZHEJIANG SUB TROPICS CROP INST

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG SUB TROPICS CROP INST
Filing Date
2026-06-17
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

There are currently no reports of aseptic bud rooting and seedling production from tung tree. Existing technologies for inducing bud rooting from tung tree hypocotyls are difficult and have low survival rates, presenting a technical bottleneck.

Method used

A method for aseptic bud rooting of tung trees is provided, which includes sterilizing the tung tree hypocotyl and culturing it in a bud induction medium to obtain apical buds. Then, the apical buds that germinate from the 12-month-old tung tree hypocotyls are inoculated into a rooting medium and cultured using an optimized rooting medium formula such as MS + KT 2.0 mg/L + NAA 0.1 mg/L + zinc oxide 0.05 mg/L.

Benefits of technology

The breakthrough in the tissue culture technology of tung tree has achieved efficient rooting, high rooting rate, and excellent seedling growth. It fills the gap in the technology of regulating the bud age of tung tree hypocotyl-induced rooting and has broad application prospects.

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Abstract

本申请公开了桐花树无菌芽生根方法,属于植物组织培养技术领域,包括如下步骤:提供桐花树胚轴,消毒后置于芽诱导培养基中培养,获得桐花树胚轴萌发的顶芽;将培养获得的12个月的桐花树胚轴萌发的顶芽离体接种到生根培养基中培养,获得生根的组培苗。本发明通过适宜生理龄期筛选结合专属生根培养基配方双重技术创新,有效解决现有桐花树组培中胚轴诱导芽生根难、成活率低的技术瓶颈,生根效率高、苗木长势优,填补国内外桐花树胚轴诱导芽龄期调控生根技术空白,应用前景广阔。
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