一种紫贻贝四倍体的制种方法
By employing a direct induction method in mussels, combined with techniques such as gonadal maturation induction, parent shellfish spawning induction, artificial insemination, and electrical stimulation, a highly efficient conversion from diploid to tetraploid was achieved, solving the problem of tetraploid preparation in shellfish and improving survival rate and induction rate.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- LUDONG UNIVERSITY
- Filing Date
- 2026-06-18
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies are difficult to efficiently prepare tetraploids in shellfish, especially in mussels, where no successful reports have been made. Furthermore, traditional methods suffer from low induction rates and long processing times.
The direct induction method was adopted, which combined gonadal maturation, parent shellfish spawning induction, artificial insemination, electrical stimulation and calcium ion carrier incubation water to achieve the direct transformation of the purple mussel from diploid to tetraploid. This included calcium-free electroporation buffer and electrical pulse stimulation to improve the efficiency of polysperm entry into eggs, and the use of calcium ion carrier to accelerate the hatching of fertilized eggs.
For the first time, tetraploid adults were successfully obtained in mollusks, improving the efficiency and survival rate of polyploid formation and solving the problems of low induction rate and long time consumption of traditional methods, laying the foundation for triploid production of mussels.
Smart Images
Figure CN122397653A_ABST