一种日本刀角瓢虫成虫无损伤性别鉴定方法及其应用

By observing the outline of the internal reproductive organs of the Japanese ladybug under a stereomicroscope, the problem of non-destructive, rapid, and accurate sex identification in existing technologies has been solved. This method achieves efficient and low-cost sex identification, which is suitable for large-scale propagation and scientific research.

CN122397689APending Publication Date: 2026-07-17INST OF PLANT PROTECTION FAAS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF PLANT PROTECTION FAAS
Filing Date
2026-06-01
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Current technologies cannot achieve rapid and accurate sex identification without damaging the Japanese ladybug. Traditional methods such as dissection and molecular markers are destructive and complex to operate, making it difficult to meet the needs of large-scale propagation and scientific research.

Method used

The sex of adult insects was determined by observing the abdominal fluoroscopic view under a stereomicroscope and identifying the shape and orientation of the fluoroscopic outline of the internal reproductive organs. The specific steps included obtaining the individuals to be identified, observing the abdominal fluoroscopic view, and determining the sex, using semi-oval and linear/triangular outline features for discrimination.

Benefits of technology

It achieves non-invasive, rapid, and accurate sex identification with a 100% accuracy rate. The operation is simple and low-cost, suitable for rapid identification of large batches. Individuals in good condition can be used for subsequent breeding and research.

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Abstract

本发明属于农业昆虫学和生物防治技术领域,涉及一种日本刀角瓢虫成虫无损伤性别鉴定方法及其应用,将日本刀角瓢虫成虫腹面朝上置于体视显微镜下,对腹部末端区域进行透视观察,获取内生殖器官的透视轮廓;当所述透视轮廓呈半卵形,轮廓主轴自腹部末端指向虫体前方中胸方向时,判定为雌性个体;所述半卵形透视轮廓为卵巢及输卵管在腹面体壁上的投影;当所述透视轮廓呈线形或三角形,轮廓自腹部末端偏向虫体右侧后足方向时,判定为雄性个体;本发明利用日本刀角瓢虫成虫羽化早期腹部体壁半透明特性、通过体视显微镜透视内生殖器官轮廓进行性别鉴定的无损伤方法及其在规模化扩繁中的应用。
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