A high-resistance compound bacterial agent based on substrate colonization and immune stimulation, its preparation method and application method

By combining Bacillus belyceae and Bacillus amyloliquefaciens with nano-protein selenium, a highly stress-resistant compound bacterial agent has been developed, solving the problems of weak colonization and unstable efficacy in the control of soil-borne diseases. This results in highly efficient and stable disease control, is suitable for various application methods, and meets the environmental protection requirements of green agriculture.

CN122397756APending Publication Date: 2026-07-17YUNNAN BOSIO BIOTECHNOLOGY CO LTD
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YUNNAN BOSIO BIOTECHNOLOGY CO LTD
Filing Date
2026-03-18
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing microbial agents have problems such as weak colonization ability, poor efficacy stability and poor stress resistance in the control of soil-borne diseases, making it difficult to adapt to different soil conditions and the needs of large-scale agriculture.

Method used

Using Bacillus belyssus and Bacillus amyloliquefaciens as core materials, combined with nano-protein selenium, a highly stress-resistant compound bacterial agent is prepared through a dual mechanism of matrix colonization and immune stimulation. The agent is then formed into a microcapsule formulation using a spray drying process, making it suitable for application methods such as drone aerial spraying and drip irrigation.

Benefits of technology

It significantly improved the colonization rate and control efficacy of rhizosphere strains, achieving highly efficient control of bacterial wilt in tomatoes and wilt in peppers. The control efficacy is highly stable, meets the environmental protection requirements of green agriculture, and is suitable for various application methods.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122397756A_ABST
    Figure CN122397756A_ABST
Patent Text Reader

Abstract

This invention relates to the field of microbial agents, aiming to solve the problems of weak colonization ability, poor imitation stability, and poor stress resistance of existing microbial agents. It provides a highly stress-resistant composite microbial agent based on substrate colonization and immune stimulation, comprising: a core material and a wall material; the core material comprises: *Bacillus bellis* and *Bacillus amyloliquefaciens*; the *Bacillus bellis* is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.36321; the *Bacillus amyloliquefaciens* has accession number CGMCC NO.36322. This invention utilizes a two-stage technology of seedling transplanting and transplanting, increasing the amount of crop rhizosphere strains transplanted by more than 10 times compared to conventional single-drenching application. Even 15-20 days after transplanting, the effective bacterial concentration can still be maintained at ≥1×10⁶ CFU / g, significantly improving transplanting efficiency and providing a solid foundation for stable control efficacy. The synergistic effect of interstrain metabolic mutualism and NPSe immune stimulation results in ≥80% control efficacy against bacterial wilt of tomatoes, reducing the incidence rate from 60% to below 12%; and ≥75% control efficacy against Fusarium wilt of peppers.
Need to check novelty before this filing date? Find Prior Art