Anti-falling essence, preparation method and application thereof

Abstract

This invention discloses an anti-hair loss essence, its preparation method, and its application, relating to the field of cosmetic technology. The anti-hair loss essence comprises the following components by weight percentage: 5.0%~10.0% anti-hair loss composition; wherein the anti-hair loss composition comprises the following raw materials: plant extracts, enzyme activators, and a reconstitution system; the enzyme activator is selected from at least one of the following: β-glucosidase from Aspergillus niger, β-glucosidase from almonds, cellulase complex enzyme, malt extract, and citrus fruit fermentation broth; the plant extract includes Platycladus orientalis leaf extract; the reconstitution system includes a moisturizing stabilizer, a chelating agent, a buffer system, and deionized water. The anti-hair loss essence, its preparation method, and its application of this invention release the active ingredients of Platycladus orientalis leaf through targeted enzymatic hydrolysis technology, constructing a plant-based compound system to achieve highly effective anti-hair loss, oil control and repair, and improved product stability.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to an anti-hair loss essence, its preparation method, and its application. Background Technology

[0002] Hair loss has become a prevalent skin appendage problem worldwide and is increasingly affecting younger people, significantly impacting consumers' physical and mental health as well as their appearance. Its core pathogenesis revolves around three main pathways: activation of 5α-reductase leads to the production of dihydrotestosterone (DHT), inducing hair follicle miniaturization; decreased proliferation and differentiation capacity of hair follicle stem cells causes hair follicle cycle disorders; and increased oxidative stress on the scalp leads to reactive oxygen species (ROS) damaging hair follicle structure and accelerating hair loss.

[0003] Plant-derived ingredients, with their gentle and multi-functional advantages, have become the core of anti-hair loss product development. Platycladus orientalis leaf extract, due to its natural anti-hair loss potential, is widely used in various hair care products. However, current technology for its application remains at the rudimentary stage of "direct addition of raw materials." Problems such as misunderstandings of the mechanism, outdated technical solutions, and a disconnect between industry, academia, and research have prevented the full release of Platycladus orientalis leaf's anti-hair loss potential, resulting in weak and unstable product efficacy. The core technological gaps and the targeted solutions of this invention are as follows: First, the conversion efficiency of precursors is low, and the utilization rate of active ingredients is insufficient, failing to fully tap the value of raw materials. Existing technologies mistakenly add over 90% of the inactive glycoside precursors (quercetin, isoquercetin, etc.) from Platycladus orientalis leaf extract directly as active ingredients, ignoring the core logic that "precursors must be hydrolyzed into free flavonoids (quercetin, kaempferol, etc.) to possess targeted anti-hair loss activity." Furthermore, they rely solely on passive conversion by endogenous β-glucosidase in the human body, which has low activity and uneven distribution, resulting in an in vitro conversion efficiency of ≤25%, scalp penetration of ≤15%, and a core active ingredient utilization rate of ≤6.7%, wasting a large amount of potential anti-hair loss ingredients. Therefore, this invention aims to construct a targeted enzymatic hydrolysis system to achieve efficient conversion of precursors and maximize the release of active ingredients, significantly improving the utilization value of raw materials.

[0004] Secondly, free flavonoids are easily oxidized and deactivated after enzymatic hydrolysis, limiting product stability and shelf life. The phenolic hydroxyl groups of free flavonoids are easily oxidized by oxygen and metal ions, leading to decreased efficacy and yellowing of the product. Existing technologies only maintain the stability of small amounts of natural free active substances by adding a single antioxidant, which cannot cope with the oxidation requirements of high concentrations of active ingredients after enzymatic hydrolysis, making it difficult to guarantee the efficacy stability of the product after long-term storage. This invention aims to construct a complex stabilization system to specifically address the oxidation of enzymatic hydrolysis products and the yellowing problem, thereby extending the half-life of active ingredients and the product's shelf life.

[0005] Third, the enzyme and raw material premixing has poor compatibility, posing a dual risk of enzyme inactivation and sensitization. Existing technologies lack enzyme-raw material compatibility design, and the addition of exogenous enzymes can easily lead to premature premixing and enzyme degradation and inactivation. This invention will solve the problem of enzyme premixing and inactivation through process optimization, achieving a balance between efficacy and safety.

[0006] Fourth, the mismatch between product form and enzymatic hydrolysis requirements hinders the industrialization of the technology. Most existing anti-hair loss products are single-chamber pre-mixed forms, lacking an isolation structure between enzymes and raw materials, making them unsuitable for the "separate placement-once mixing" logic required for enzymatic hydrolysis. A few dual-chamber or cyclodextrin-encapsulated products are only used for ingredient preservation or simple separation, lacking precise ratios, efficient mixing, and triggering conversion designs for enzymes and precursors, thus failing to support the implementation of enzymatic hydrolysis technology. This invention aims to develop innovative product forms suitable for industrialization, enabling on-the-spot preparation and precise conversion of enzymes and raw materials, thus paving the way for the industrialization of the technology.

[0007] Fifth, the enzyme activation conditions and ratios are unclear, resulting in insufficient stability of the conversion effect. Existing technologies have not clearly defined the role of β-glucosidase as an anti-desorption activator, nor have they systematically explored the optimal parameters for its compatibility with Platycladus orientalis leaf precursors—there is a lack of optimized data on the pH range, temperature range, and ionic environment required for enzyme activation, and the precise ratios of enzyme, precursor, buffer system, and stabilizer are also unclear, leading to low enzyme activation efficiency and large fluctuations in conversion effects. This invention will clarify the core parameters of enzymatic hydrolysis, establish a scientific ratio system, and achieve targeted and efficient conversion of precursors.

[0008] In summary, existing technologies have not formed an integrated solution encompassing "mechanism understanding, component transformation, stable retention, and morphological adaptation," thus failing to address the core pain points in the application of Platycladus orientalis leaf extract. This invention addresses these gaps by constructing a targeted enzymatic hydrolysis system, a complex stabilization system, and innovative product forms, achieving a breakthrough from "passively using raw materials" to "actively creating active ingredients," filling the industry gap in the in-depth development and efficient utilization of Platycladus orientalis leaf extract.

[0009] Furthermore, the core bottleneck in the implementation of the enzymatic hydrolysis anti-hair loss technology of this invention lies in the contact control between the enzyme and the Platycladus orientalis leaf extract. This enzymatic hydrolysis method is an industry first; previously, no anti-hair loss products had this design, so there was no need to consider the contact issue between the enzyme and the raw materials; they simply added the Platycladus orientalis leaf extract directly to the formula. However, this invention needs to solve the entirely new problem of "safe storage of the enzyme and precise initiation of the enzymatic hydrolysis reaction": the enzyme must avoid being mixed with the liquid raw materials in advance, otherwise it will cause enzyme protein degradation and inactivation, premature oxidation of active ingredients, leading to a sharp drop in enzymatic hydrolysis efficiency and failing to realize the technological advantages. Existing anti-hair loss products are all designed for "direct addition of plant extracts," which completely fails to meet the core requirements of enzymatic hydrolysis technology and generally suffers from four major compatibility defects: First, the single-chamber pre-mixed form is the main type, which can only accommodate the direct addition of plant extracts, without the design to isolate enzymes from raw materials, and cannot adapt to the "separate placement-fresh mixing" logic required for enzymatic hydrolysis; Second, a few dual-chamber products only achieve simple physical separation to distinguish different plant extract components or efficacy systems, without the precise ratio design of enzymes and precursors (the ratio error often exceeds ±5%) and efficient mixing structure, and are completely lacking the ability to adapt to enzymatic hydrolysis reactions; Third, cyclodextrin-encapsulated products are only for the preservation of active ingredients and are used to delay the oxidation of trace amounts of free active substances in plant extracts, without the design to meet the reaction requirements of enzymes and precursors, and cannot match the reaction rhythm of enzymatic hydrolysis technology; Fourth, industrial compatibility is only for traditional plant extract formulas, and cannot support the new technical route of "enzymatic hydrolysis + separate placement," making it difficult to achieve large-scale implementation.

[0010] Current single-use anti-hair loss serums are all pre-formulated single-chamber plant extracts without enzymatic hydrolysis design. They rely solely on adding high doses of plant extracts to compensate for insufficient active ingredients, resulting in high costs and limited efficacy. Furthermore, after 3 months of storage, the retention rate of trace free active substances is ≤32%, and they are prone to yellowing and odor. A few double-chamber single-use serums are only used to separate different plant extracts, and the packaging barrier properties only meet the storage requirements of conventional ingredients, failing to meet the stringent requirements of enzyme powder for moisture and oxygen protection. The foldable connection design only considers sealing performance and does not take into account the mixing requirements of enzyme powder and base, resulting in a poor user experience and completely lacking the adaptability for enzymatic hydrolysis reactions.

[0011] Existing cyclodextrin-encapsulated anti-hair loss products are all designed with "whole plant extract encapsulation," with the core purpose of delaying the oxidation of trace amounts of free active substances in plant extracts. They lack the concept of enzymatic hydrolysis technology and have never considered the reaction requirements between enzymes and precursors. The encapsulation materials are only selected for the preservation of plant extracts, resulting in low encapsulation rates (≤80%), and their solubility characteristics are only suitable for the release of conventional components, completely failing to meet the isolation-trigger release requirements of enzyme powders. The encapsulation process is complex and often requires the assistance of organic solvents, posing a risk of solvent residue. Improper microcapsule particle size control results in a grainy feel after application and cannot achieve precise contact reaction between enzymes and precursors, making them completely incompatible with enzymatic hydrolysis technology.

[0012] To overcome the challenges of storage and reaction adaptation in enzymatic hydrolysis technology, this invention adopts the core design concept of "isolated storage of enzymes and liquid raw materials, and precise contact initiation of enzymatic hydrolysis during use," and develops a novel packaging form specifically adapted to particular scenarios. Through a full-chain design of "physical isolation (separate placement of enzymes and liquid raw materials) - precise triggering (controllable mixing during use) - efficient enzymatic hydrolysis (matching enzymatic hydrolysis reaction parameters) - stable retention (ensuring the efficacy of active ingredients)," it achieves safe storage of enzymes and precise initiation of enzymatic hydrolysis reactions. This solves the core problems of enzyme inactivation due to premixing with raw materials and premature oxidation of active substances, while ensuring that enzymatic hydrolysis technology fully performs its functions. At the same time, it takes into account both skin feel and the needs of industrial mass production, forming an adapted enzymatic hydrolysis technology. Summary of the Invention

[0013] To address the aforementioned technical problems, this invention provides an anti-hair loss essence, its preparation method, and its application, aiming to solve the following technical issues: 1. Solving the problem of low conversion efficiency and insufficient utilization of active ingredients in glycoside precursors from Platycladus orientalis leaves, maximizing the release of potential anti-hair loss components, and enhancing the utilization value of raw materials; 2. Solving the problem of easy oxidation and inactivation of free flavonoid active substances after enzymatic hydrolysis, leading to product yellowing, and extending the half-life of active ingredients and product shelf life; 3. Solving the problem of premature enzyme inactivation and degradation due to pre-mixing of enzymes and raw materials, and the risk of scalp sensitization caused by enzyme protein residue, balancing conversion efficiency and product safety; 4. Solving the problem of existing product forms lacking... This invention addresses the issues of enzyme stability and efficient release of active substances, developing innovative product forms suitable for industrial applications to achieve on-the-spot preparation and precise conversion; 5. It solves the problem that existing technologies lack clear activation conditions and precise ratios for enzyme activators (β-glucosidase), breaking through the bottleneck of activation parameters and ratios—existing technologies have not explored the optimal pH range, temperature adaptation range, and ionic environment required for enzyme activation, and have not clearly defined the precise ratios of enzyme with precursors, buffer systems, and stabilizers, resulting in low enzyme activation efficiency and unstable conversion effects. This invention needs to clarify the enzyme activation conditions and scientific ratios suitable for Platycladus orientalis leaf precursors to achieve targeted and efficient conversion. 6. This invention addresses the issue of insufficient activity stability of enzymatic activators (β-glucosidase), overcoming the bottleneck of activity maintenance during storage and use. Existing technologies cannot effectively maintain enzyme activity in complex cosmetic systems (including surfactants, preservatives, and polyols) and during long-term storage. Enzyme structure is easily damaged and activity decays due to environmental changes. This invention requires the construction of a dedicated stable system to ensure the activity stability of enzymes during storage and use, ensuring that enzymatic hydrolysis efficiency consistently meets standards. 7. This invention addresses the issue of existing technologies lacking a targeted combination system of anti-hair loss active plant extracts, overcoming the bottleneck of synergistic adaptation of multiple extracts. Existing technologies mostly involve adding single plant extracts, without systematically exploring the optimal compounding ratio, specification compatibility, and efficacy synergistic mechanism of extracts such as Platycladus orientalis, Ligustrum lucidum, and Polygonum multiflorum. This fails to form a closed loop of "precursor supply - efficacy enhancement - activity protection." This invention requires exploring the optimal combination system of anti-hair loss active plant extracts, clarifying the compounding ratio and specification limitations of the three components, and achieving a balance between efficacy synergy and cost and safety.

[0014] Specifically, the following technical solutions are included: In a first aspect, an anti-hair loss essence is provided, comprising the following components by weight percentage: 5.0%~10.0% anti-hair loss composition; wherein, the anti-hair loss composition comprises the following raw materials: plant extracts, enzyme activators, and a reconstitution system; the enzyme activator is selected from at least one of the following: β-glucosidase derived from Aspergillus niger, β-glucosidase derived from almonds, cellulase complex enzyme, malt extract, and citrus fruit fermentation broth; the plant extract includes Platycladus orientalis leaf extract; the reconstitution system comprises a moisturizing stabilizer, a chelating agent, a buffer system, and deionized water.

[0015] The arborvitae leaf extract contains glycoside precursors, including at least one of quercetin, isoquercetin, afostigmoside, and myricetin. The anti-hair loss composition contains free flavonoid active ingredients, including at least one of quercetin, kaempferol, and myricetin.

[0016] Preferably, the enzyme activator is selected from at least one of the following: β-glucosidase derived from Aspergillus niger, malt extract, and citrus fruit fermentation broth.

[0017] Furthermore, the hair loss prevention essence also includes the following components by weight percentage: oil control agent 1.0%~2.0%, penetration enhancer 3.0%~5.0%, moisturizing and soothing agent 0.5%~1.5%, film-forming agent 0.1%~0.3%, pH adjuster 0.1%~0.2%, preservative 0.5%~0.8%, stabilizer 0.05%~0.1%, and other auxiliary agents 0.05%~0.15%.

[0018] Furthermore, the oil-controlling agent is betaine salicylate, the penetration enhancer is 1,3-propanediol, the moisturizing and soothing agent is panthenol (vitamin B5), the film-forming agent is sodium hyaluronate (molecular weight 100kDa), the pH adjuster is citric acid or triethanolamine, the preservative is composed of phenoxyethanol and ethylhexylglycerin in a mass ratio of 1:1, the stabilizer is disodium EDTA, and the other auxiliary agents are fragrance (no essential oils / low allergens).

[0019] Preferably, the anti-hair loss essence is composed of the following components by weight percentage: 5.0%~10.0% anti-hair loss composition, 1.0%~2.0% oil control agent, 3.0%~5.0% penetration enhancer, 0.5%~1.5% moisturizing and soothing agent, 0.1%~0.3% film-forming agent, 0.1%~0.2% pH adjuster, 0.5%~0.8% preservative, 0.05%~0.1% stabilizer, 0.05%~0.15% other auxiliary agents, and the balance being deionized water.

[0020] Furthermore, the plant extracts also include privet fruit extract and / or fleeceflower root extract.

[0021] Furthermore, in the anti-hair loss composition, the plant extracts also include privet fruit extract and fleeceflower root extract, and the mass ratio of the arborvitae leaf extract, privet fruit extract and fleeceflower root extract is 10:2:1.

[0022] Furthermore, in the hair loss prevention composition, the enzyme activator is β-glucosidase derived from Aspergillus niger, and the mass ratio of the β-glucosidase derived from Aspergillus niger to the Platycladus orientalis leaf extract is 1:(10-20).

[0023] Preferably, the mass ratio of the β-glucosidase derived from Aspergillus niger to the extract of Platycladus orientalis leaves is 1:15.

[0024] Furthermore, in the hair loss prevention composition, the enzyme activator is composed of malt extract and citrus fruit fermentation broth; the weight ratio of the malt extract to the citrus fruit fermentation broth is (4-8):(6-12).

[0025] Furthermore, the enzyme activator is composed of β-glucosidase from Aspergillus niger, malt extract, and citrus fruit fermentation broth, wherein the mass ratio of β-glucosidase from Aspergillus niger, malt extract, and citrus fruit fermentation broth is 2:6:9.

[0026] Preferably, the enzyme activator is malt extract; based on the total mass of the enzyme reconstitution system composed of the enzyme activator and the reconstitution system, the content of the malt extract is 4%-8%.

[0027] Preferably, the enzyme activator is citrus fruit fermentation broth; based on the total mass of the enzyme reconstitution system composed of the enzyme activator and the reconstitution system, the content of the citrus fruit fermentation broth is 6%-12%.

[0028] Preferably, the enzyme activator is composed of malt extract and citrus fruit fermentation broth; based on the total mass of the enzyme reconstitution system composed of the enzyme activator and the reconstitution system, the content of the malt extract is 4%-8%, and the content of the citrus fruit fermentation broth is 6%-12%.

[0029] Preferably, the enzyme activator is composed of malt extract and citrus fruit fermentation broth; based on the total mass of the enzyme reconstitution system composed of the enzyme activator and the reconstitution system, the content of the malt extract is 6% and the content of the citrus fruit fermentation broth is 9%.

[0030] Preferably, based on the total mass of the enzyme reconstitution system consisting of the enzyme activator and the reconstitution system, the enzyme activator accounts for 2.0% of the mass percentage of the enzyme reconstitution system.

[0031] Furthermore, the enzyme activator is composed of β-glucosidase from Aspergillus niger, malt extract, and citrus fruit fermentation broth, wherein the mass ratio of β-glucosidase from Aspergillus niger, malt extract, and citrus fruit fermentation broth is 2:6:9.

[0032] Preferably, the enzyme activator is composed of β-glucosidase from Aspergillus niger, malt extract and citrus fruit fermentation broth. Based on the total mass of the enzyme reconstitution system composed of the enzyme activator and the reconstitution system, the content of β-glucosidase from Aspergillus niger is 2%, the content of malt extract is 6%, and the content of citrus fruit fermentation broth is 9%.

[0033] Furthermore, in the hair loss prevention composition, the enzyme activator is composed of almond-derived β-glucosidase, malt extract and citrus fruit fermentation broth, and the mass ratio of almond-derived β-glucosidase, malt extract and citrus fruit fermentation broth is (0.10-0.20):(4-8):(6-12).

[0034] Furthermore, in the hair loss prevention composition, the enzyme activator is composed of cellulase complex enzyme, malt extract and citrus fruit fermentation broth, and the mass ratio of cellulase complex enzyme, malt extract and citrus fruit fermentation broth is (0.20-0.40):(4-8):(6-12).

[0035] Furthermore, in the hair loss prevention composition, the enzyme activator is composed of almond-derived β-glucosidase, cellulase complex enzyme, malt extract and citrus fruit fermentation broth, and the mass ratio of almond-derived β-glucosidase, cellulase complex enzyme, malt extract and citrus fruit fermentation broth is (0.10-0.20):(0.20-0.40):(4-8):(6-12).

[0036] Furthermore, in the anti-hair loss composition, the moisturizing stabilizer is propylene glycol, the chelating agent is disodium EDTA, and the buffer system is citrate-sodium citrate buffer solution.

[0037] Furthermore, in the enzyme reconstitution system, the enzyme activator accounts for 2.0%-17% of the mass percentage of the enzyme reconstitution system, the moisturizing stabilizer accounts for 3%-8% of the mass percentage of the enzyme reconstitution system, the chelating agent accounts for 0.01%-0.1% of the mass percentage of the enzyme reconstitution system, and the remainder is deionized water; the buffer system is used to adjust the pH value of the enzyme reconstitution system to 5.0-5.2.

[0038] Preferably, in the enzyme reconstitution system, the enzyme activator accounts for 2.0% of the mass percentage of the enzyme reconstitution system, the moisturizing stabilizer accounts for 5% of the mass percentage of the enzyme reconstitution system, the chelating agent accounts for 0.05% of the mass percentage of the enzyme reconstitution system, and the remainder is deionized water; the buffer system is used to adjust the pH value of the enzyme reconstitution system to 5.2.

[0039] Secondly, a method for preparing the anti-hair loss essence according to the first aspect is provided, comprising the following steps: (1) Preparation of anti-hair loss composition: The enzyme activator is mixed with the reconstitution system to form an enzyme reconstitution system, and then mixed with the plant extract. The enzymatic reaction is carried out at a temperature of 25-42℃ and an enzymatic hydrolysis time of 4-7 hours to obtain the anti-hair loss composition. (2) Aqueous phase preparation: Add deionized water to the mixing tank, heat to 45~50℃, add the penetration promoter, stabilizer and oil control agent in sequence, stir until completely dissolved, and keep warm for later use; (3) Addition of functional components: Cool down to 35~40℃, add the anti-hair loss composition, stir evenly at a speed of 800~1000r / min, and keep warm for 10min; (4) Addition of moisturizing and film-forming system: Add moisturizing and soothing agent and film-forming agent, and stir at a low speed of 500r / min for 15min; (5) Fragrance and shaping: Adjust the pH to 5.0~5.5 with pH adjuster, add preservatives and other additives, stir evenly; filter through a 0.22μm filter membrane to obtain the anti-hair loss essence.

[0040] Furthermore, the preparation method of the aforementioned anti-hair loss essence includes the following steps: (1) Preparation of anti-hair loss composition: The enzyme activator is mixed with the reconstitution system to form an enzyme reconstitution system, and then mixed with the plant extract. The enzymatic reaction is carried out at a temperature of 25-40℃ and an enzymatic hydrolysis time of 2-6 hours to obtain the anti-hair loss composition. (2) Aqueous phase preparation: Add deionized water to the mixing tank, heat to 45~50℃, add the penetration promoter, stabilizer and oil control agent in sequence, stir until completely dissolved, and keep warm for later use; (3) Addition of active ingredients: Cool down to 35~40℃, add the anti-hair loss composition, stir evenly at a speed of 800~1000r / min, keep warm for 10min to ensure that the active ingredients are evenly dispersed and there is no local aggregation; (4) Adding moisturizing and film-forming system: Add moisturizing and soothing agent and film-forming agent, stir at a low speed of 500r / min for 15min to avoid the generation of bubbles and ensure that the film-forming agent is fully dissolved; (5) Fragrance and shaping: Adjust the pH to 5.0~5.5 with pH adjuster, add preservatives and other additives, stir evenly; filter through 0.22μm filter membrane (to remove impurities and air bubbles), aseptically fill, and quality inspection to obtain anti-hair loss essence.

[0041] Thirdly, a single-use, pre-mixed anti-hair loss product is provided, comprising the anti-hair loss essence raw material described in the first aspect, including a dual-compartment packaging container, wherein the dual-compartment packaging container physically isolates the enzyme activator in the raw material from the remaining raw materials.

[0042] Preferably, in the single-use, pre-mixed anti-hair loss product, the dual-compartment packaging container is a single-use, double-compartment, easily foldable single-use vial. The vial is internally divided into a first compartment and a second compartment. The first compartment contains the remaining raw materials, and the second compartment contains the enzyme activator. An easily foldable connecting part is provided between the first compartment and the second compartment.

[0043] Fourthly, a cyclodextrin-encapsulated trigger-type anti-hair loss product is provided, comprising the raw materials of the anti-hair loss essence described in the first aspect, wherein the enzyme activator in the raw materials is encapsulated by cyclodextrin to form microcapsule particles, and the microcapsule particles are dispersed in an essence base composed of the remaining raw materials.

[0044] Compared with the prior art, the present invention has the following beneficial effects: 1. The utilization rate of raw materials has been significantly improved, solving the common industry problem of low conversion efficiency of precursors.

[0045] By constructing a targeted enzymatic hydrolysis system, using β-glucosidase from Aspergillus niger as the core enzyme activator, combined with a citrate-sodium citrate buffer system and a reconstitution system composed of propylene glycol and disodium EDTA, inactive glycoside precursors in Platycladus orientalis leaf extract are efficiently converted into free flavonoids with targeted anti-hair loss activity. The utilization rate and yield of active ingredients are increased by tens of times. While maintaining the same anti-hair loss effect, the amount of Platycladus orientalis leaf extract used can be significantly reduced, significantly lowering raw material costs and resource consumption, and solving the industry-wide common problem of "difficult release of potential active ingredients" in Platycladus orientalis leaf raw materials.

[0046] 2. The anti-hair loss effect has been greatly upgraded, with fast onset and long-lasting effect.

[0047] The free flavonoids generated after enzymatic hydrolysis have significantly increased scalp penetration and a greatly shortened onset time. In vitro experiments show that the anti-hair loss essence containing the anti-hair loss composition of this invention has a significantly higher total inhibition rate against type I and type II 5α-reductase than un-enzymatically hydrolyzed products and similar commercially available products. Human experimental results show that subjects using the anti-hair loss essence of this invention have significantly higher rates of hair loss reduction, hair density improvement, and hair root tensile strength improvement than the control group, achieving a dual effect of inhibiting hair follicle miniaturization from the root and prolonging the hair follicle growth phase.

[0048] 3. Construct a compound system of plant extracts with synergistic effects of "Platycladus orientalis leaves, Ligustrum lucidum and Polygonum multiflorum".

[0049] This invention optimized the optimal mass ratio of Platycladus orientalis leaf extract, Ligustrum lucidum extract, and Polygonum multiflorum extract through orthogonal experiments. This compound system forms a synergistic closed loop of "precursor supply - efficacy enhancement - activity protection": oleanolic acid in Ligustrum lucidum extract synergistically enhances the inhibition rate of 5α-reductase and activates the hair follicle stem cell pathway; stilbene glycosides in Polygonum multiflorum extract provide potent antioxidant protection, forming a dual antioxidant protective network with myricetin, effectively prolonging the retention time of active ingredients. This system possesses multiple effects, including anti-inflammatory, antioxidant, and scalp barrier repair.

[0050] 4. The combined stability system and the separate product form work together to ensure product stability and safety, significantly improving product stability and safety.

[0051] By employing a composite stabilizing system composed of propylene glycol and disodium EDTA, combined with a single-use dual-compartment packaging design that physically isolates the enzyme activator from the essence base, the problem of easy oxidation and inactivation of free flavonoids after enzymatic hydrolysis and enzyme activity attenuation caused by premixing the enzyme with raw materials is effectively solved. The retention rate of active ingredients and the storage stability of enzyme activity are significantly improved. Simultaneously, the inactivation process after enzymatic hydrolysis and the separate storage design of the enzyme activator eliminate the risk of sensitization that may be caused by enzyme protein residue. The formula is gentle and suitable for sensitive scalps, and the product does not show obvious yellowing.

[0052] 5. Innovative product forms enable "on-demand matching and precise conversion," adapting to diverse scenarios and facilitating industrial mass production.

[0053] The single-use, pre-mixed anti-hair loss product provided by this invention adopts a single-use, double-compartment, easily foldable bottle design, physically isolating the enzyme activator from the essence base. Upon use, breaking the bottle and mixing triggers the enzymatic reaction, achieving a complete "separate placement-pre-mixing-precise enzymatic hydrolysis" design. A cyclodextrin-encapsulated trigger-type product is also provided, using microencapsulation technology to disperse the enzyme activator within the essence base, with release triggered by rubbing. This series of products requires no modification to the core production line; only adjustments to the filling or microencapsulation preparation station are needed for large-scale mass production, making it highly valuable for industrial applications.

[0054] 6. It improves scalp health in multiple ways, expanding its effects to include "anti-hair loss + oil control + repair".

[0055] This invention's anti-hair loss essence utilizes an "oil-free formula + high penetration + targeted nourishment" system. The high concentration of active ingredients after enzymatic hydrolysis not only enhances its anti-hair loss efficacy but also simultaneously improves multiple scalp issues: regulating oil balance, significantly reducing scalp sebum secretion; promoting scalp microcirculation, replenishing nutrients to hair follicles; and repairing the scalp barrier, significantly improving transepidermal moisture loss and noticeably reducing scalp redness. It achieves an integrated effect of "anti-hair loss + oil control + scalp barrier repair," solving the pain points of traditional anti-hair loss essences that are limited in function and leave the scalp feeling sticky. Detailed Implementation

[0056] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0057] It should be understood that, when used in this specification and the appended claims, the terms "comprising" and "including" indicate the presence of the described features, integrals, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integrals, steps, operations, elements, components and / or collections thereof.

[0058] It should also be understood that the terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to limit the invention. As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” are intended to include the plural forms unless the context clearly indicates otherwise.

[0059] It should also be further understood that the term "and / or" as used in this specification and the appended claims refers to any combination of one or more of the associated listed items and all possible combinations, and includes such combinations.

[0060] To better understand the technical content of the present invention, the technical solution of the present invention will be further introduced and explained below with reference to specific embodiments.

[0061] Unless otherwise stated, the concentration (%) referred to in this invention refers to mass concentration, the content (%) refers to mass content, and the amount added (%) refers to mass percentage.

[0062] I. The enzyme activator is β-glucosidase derived from Aspergillus niger, and the plant extract is Platycladus orientalis leaf extract. (1) Enzyme screening experiment Enzyme activity was determined using the DNS (3,5-dinitrosalicylic acid) method. Using glucose as a standard, one enzyme activity unit (U) was defined as the amount of enzyme required to catalyze the production of 1 μmol of glucose from the substrate (Thuja orientalis leaf extract) within 1 minute under conditions of pH 5.5 and a temperature of 25-40℃. The Thuja orientalis leaf extract contained 100% solids, more than 0.1% quercetin, and more than 1% total flavonoids.

[0063] Five potential glycoside hydrolases were initially screened: β-glucosidase from Aspergillus niger, β-glucosidase from almond, cellulase complex, pectinase, and xylanase. The core evaluation indicators were "precursor conversion efficiency, active ingredient yield, and compatibility with cosmetic systems." Each experiment had three replicates, and the experimental conditions were standardized: substrate concentration 45%, enzyme addition 0.2%, pH 5.5, temperature 25-40℃, and hydrolysis time 4 hours. The results of the enzyme screening experiments are shown in Table 1 below. Table 1 Results of enzyme screening experiments As shown in Table 1, pectinase and xylanase have no specific catalytic effect on the precursors of arborvitae leaf glycosides, and the total conversion efficiency is ≤10%, so they do not have the application value of enzymatic hydrolysis of arborvitae leaves and are directly excluded. Almond-derived β-glucosidase and cellulase complex enzymes can be used independently or alone as effective enzymes for the enzymatic hydrolysis of Platycladus orientalis leaf extract; when combined, they can form a synergistic combination and can also be used as an independent enzymatic hydrolysis system without relying on other enzyme components.

[0064] Among them, almond-derived β-glucosidase has high substrate specificity and can be efficiently hydrolyzed alone; cellulase complex enzyme has both cell wall disruption and glycoside hydrolysis capabilities and can complete enzymatic hydrolysis independently; when the two are combined, the total precursor conversion efficiency is ≥80%, achieving complementary advantages and the conversion efficiency is significantly higher than that of single cellulase complex enzyme, showing good application potential.

[0065] β-glucosidase derived from Aspergillus niger does not require metal ion activation, has the strongest substrate specificity, the highest conversion efficiency, the best cosmetic compatibility and safety, and the best overall performance, making it the preferred core enzyme.

[0066] In addition, malt extract and citrus fruit fermentation broth, two raw materials with enzymatic hydrolysis capabilities and eligible for registration, were selected to verify their release efficiency of active substances from Platycladus orientalis leaf extract. The malt extract was enzyme-active, containing ≥500 U / g β-glucosidase, ≥300 U / g amylase, ≥40% total solids, and pH 4.5-5.5. The citrus fruit fermentation broth was fermented, containing ≥400 U / g pectinase, ≥350 U / g β-glucosidase, ≤5% total acid, pH 3.5-4.5, and no alcohol residue.

[0067] (2) Enzymatic hydrolysis process Through single-factor experiments and orthogonal experiments (L9(3) 4 The systematic exploration of enzymatic hydrolysis methods was completed, clarifying the optimal schemes for enzyme types, reaction parameters, and enzyme powder reconstitution systems. This ensured that the hydrolysis efficiency, active ingredient yield, and enzyme activity stability all met the standards for industrial application. The specific exploration process is as follows: ① The process of exploring enzyme types Five potential glycoside hydrolases were initially screened: β-glucosidase from Aspergillus niger, β-glucosidase from almonds, cellulase complex, pectinase, and xylanase. The core evaluation indicators were "precursor conversion efficiency, active ingredient yield, and compatibility with the cosmetic system." Each experiment had three replicates, with standardized experimental conditions: substrate concentration 45%, enzyme addition 0.2%, pH 5.5, temperature 40℃, and hydrolysis time 4 hours. Results showed that pectinase and xylanase had no specific catalytic effect on thuja glycoside precursors, with a total conversion efficiency ≤10%, and were directly excluded. While the cellulase complex showed weak conversion ability, its low β-glucosidase activity (only 200 U / g) resulted in a total conversion efficiency ≤72%, and it was easily inactivated by binding with polyols in the formulation. Almond-derived β-glucosidase had a conversion efficiency ≥76%, but required Mn... 2+ Activation is required, but it is incompatible with cationic surfactants and has a low risk of allergenicity. β-glucosidase derived from Aspergillus niger does not require metal ion activation, has strong specificity for precursors such as quercetin and isoquercetin, has a total conversion efficiency of ≥88%, and has excellent compatibility with commonly used cosmetic ingredients and no allergenicity. It was ultimately identified as the core enzyme.

[0068] ② The process of exploring reaction parameters Using β-glucosidase derived from Aspergillus niger as the research subject, the parameter range was determined through single-factor experiments, and then the optimal value was optimized through orthogonal experiments (factors: temperature, time, enzyme dosage; levels: temperature 35 / 38 / 41℃, time 3 / 4 / 5h, enzyme dosage 0.15% / 0.2% / 0.25%), as detailed below: Temperature: At 35℃, enzyme activity is insufficient, with a total conversion efficiency of only 75%. Good enzymatic activity is maintained within the range of 25-40℃, with 38-40℃ being the peak in vitro activity range. At this temperature, the enzyme's active center structure is stable, and the conversion efficiency is ≥88%. This data serves as the benchmark parameter for optimizing enzymatic hydrolysis efficiency in the laboratory. Considering everyday room temperature use, this invention optimizes the enzyme stabilization system, ensuring the enzyme maintains high activity at scalp physiological temperatures (25-40℃, close to the actual effective temperature after contact with the scalp at room temperature), achieving a conversion efficiency ≥85%. No additional heating is required, perfectly suited for home use at room temperature. At enzymatic hydrolysis temperatures of 41℃ and above, the enzyme protein's spatial structure is destroyed, activity rapidly declines, and quercetin degradation rate is ≥10%. Therefore, the core effective temperature range for practical applications is clearly defined as the scalp physiological temperature range of 25-40℃, with a laboratory-optimized temperature of 38-40℃. The final product requires no deliberate temperature control, aligning with daily care habits.

[0069] Time: The reaction did not reach saturation within 3 hours of enzymatic hydrolysis, the precursor was not fully converted, and the conversion efficiency was ≤75%; the reaction reached saturation at 4 hours, the conversion efficiency was 88%, and the concentration of active ingredients reached its peak; extending to 5 hours, the conversion efficiency only increased by 1.5%, and the active ingredients were slightly reduced due to oxidation. Considering both efficiency and cost, the enzymatic hydrolysis time was determined to be 4 hours.

[0070] Enzyme addition amount: When the addition amount is 0.15%, the enzyme amount is insufficient, resulting in a conversion efficiency of 78%; when the enzyme and precursor concentration is matched at 0.2%, the conversion efficiency is 88% and the concentration of active ingredients meets the standard; when the addition amount is 0.25%, the conversion efficiency is only increased by 2%, but the residual amount of enzyme protein increases, making subsequent inactivation more difficult. The optimal enzyme addition amount is determined to be 0.2% (mass ratio of 1:15 with Platycladus orientalis leaf extract).

[0071] (3) The exploration process of enzyme powder reconstitution system formulation In this invention, the enzyme activator is stored only in the form of enzyme powder (lyophilized powder) or applied after being encapsulated with cyclodextrin, and is not used in the form of enzyme solution—enzyme solutions have extremely poor stability and are greatly affected by temperature and formulation components, which cannot meet the requirements of industrial storage and application. The core of this exploration is to optimize the enzyme powder reconstitution system to ensure that the enzyme powder is rapidly activated and stably effective after being added to the product base, and to clarify the enzyme powder storage conditions and specifications, as follows: ① Buffer system screening experiment Screening objective: To screen buffer systems that can maintain the optimal pH for enzymatic hydrolysis, promote enzyme powder dissolution, and ensure storage stability. All test concentrations were 0.05 mol / L. Experimental results are shown in Table 2 below. Table 2 Results of buffer system screening experiments ② Screening experiment for moisturizing stabilizers Screening objective: To screen for moisturizing stabilizers that can inhibit enzyme protein aggregation and improve enzyme powder dispersibility. The addition amount was 5% (based on the total mass of the enzyme reconstitution system). The experimental results are shown in Table 3 below. Table 3 Results of the screening experiment for moisturizing stabilizers ③ Chelating agent screening experiment Screening objective: To screen chelating agents that can chelate trace metal ions and protect enzyme structure. The addition amount was 0.05% (based on the total mass of the enzyme reconstitution system). The experimental results are shown in Table 4 below. Table 4 Results of chelating agent screening experiments (4) Determination of enzyme powder specifications and reconstitution formula Enzyme powder (lyophilized powder) specifications: β-glucosidase derived from Aspergillus niger, prepared using a modified freeze-drying process (freezing temperature -55℃, vacuum degree -0.12MPa, segmented drying time 30h, pre-freezing at low temperature for 8h followed by gradient temperature drying), enzyme activity 1200U / g, moisture content ≤2.5%, packaged in multi-layer composite vacuum aluminum foil (with built-in food-grade desiccant and oxygen absorbent, 0.1-1kg / bag, suitable for industrial feeding), packaging barrier properties: oxygen permeability ≤0.5cm. 3 / (m 2 • 24h • 0.1MPa), moisture permeability ≤ 0.3g / (m 2 •24h).

[0072] Storage conditions: Optimized for stable storage at room temperature. Unopened, store in a cool, dry environment below 25℃, protected from light, for a shelf life of 12 months (activity retention ≥75%). If stored in a cool place (15-20℃), the shelf life can be extended to 18 months (activity retention ≥80%). Once opened, use within 15 days at room temperature and in a dry environment. Refrigeration at 4℃ + drying can extend the shelf life to 1 month (expired enzyme activity ≤40%). This specification, through process optimization and packaging upgrades, breaks free from reliance on low-temperature storage, making it more suitable for industrial warehousing, transportation, and end-consumer usage scenarios in the cosmetics industry.

[0073] Example 1 A hair loss prevention composition comprises the following raw materials: a plant extract (Thuja orientalis leaf extract), an enzyme activator (β-glucosidase derived from Aspergillus niger), and a reconstitution system; said reconstitution system includes a moisturizing stabilizer (propylene glycol), a chelating agent (disodium EDTA), a buffer system (citric acid-sodium citrate buffer), and deionized water. The mass ratio of the β-glucosidase derived from Aspergillus niger to the Thuja orientalis leaf extract is 1:15.

[0074] The method for preparing the anti-hair loss composition includes the following steps: The enzyme activator is mixed with the reconstitution system (the enzyme activator is mixed with deionized water, moisturizing stabilizer, and chelating agent, and the pH value is adjusted with a buffer system) to prepare an enzyme reconstitution system, which is then mixed with the plant extract. The enzymatic reaction is carried out at a temperature of 38°C and an enzymatic hydrolysis time of 4 hours to obtain the hair loss prevention composition.

[0075] The enzyme activator is provided in the form of enzyme powder. In the enzyme reconstitution system, the enzyme powder accounts for 2.0% of the total mass, the propylene glycol accounts for 5.0% of the total mass, and the disodium EDTA accounts for 0.05% of the total mass. The pH of the citrate-sodium citrate buffer is adjusted to 5.2, and the remainder is deionized water.

[0076] Comparative Example 1 An extract of Platycladus orientalis leaves, without enzymatic hydrolysis.

[0077] Performance testing: Using Comparative Example 1 as the control group, the efficacy of Example 1 was verified by HPLC, human trials, and cost accounting. Specific data are shown in Tables 5-7 below: Table 5. Results of the active ingredient utilization rate test in Example 1 (with Comparative Example 1 as the control group). Table 6. Anti-hair loss effects in human trials of Example 1 and Comparative Example 1 Table 7. Raw material savings and industrialization benefits of Example 1 and Comparative Example 1 II. The enzyme activator is at least one of malt extract and citrus fruit fermentation broth, and the plant extract is arborvitae leaf extract. Two raw materials with enzymatic hydrolysis capabilities and registration requirements were selected: malt extract and citrus fruit fermentation liquid. Single-raw material enzymatic hydrolysis and compound raw material enzymatic hydrolysis experiments were designed to optimize the content of raw materials and verify their effects on the release efficiency of active substances in arborvitae leaf extract, anti-hair loss efficacy and scalp care effect.

[0078] (I) Specifications of core raw materials (% refers to mass content) 1. Arborvitae leaf extract: commercial powder, total content of quercetin + isoquercetin + afotiam + myricetin ≥15%, solids ≥30%, moisture ≤8%; 2. Malt extract: Enzymatic type, containing β-glucosidase ≥500U / g, amylase ≥300U / g, total solids ≥40%, pH 4.5-5.5; 3. Citrus fruit fermentation broth: fermented type, containing pectinase ≥400U / g, β-glucosidase ≥350U / g, total acid ≤5%, pH 3.5-4.5, and no alcohol residue; 4. Special excipients for compartmentalization: citrate-sodium citrate buffer, propylene glycol, disodium EDTA (all cosmetic grade, suitable for independent storage and compounding stability of enzyme hydrolysate); 5. Finished product formulation excipients: conventional cosmetic-grade surfactants, moisturizers, preservatives, etc. (consistent with the formulations of commercially available anti-hair loss products, and compatible with the compounded system).

[0079] (II) Core Evaluation Indicators of the Experiment 1. Specific indicators for enzymatic hydrolysate (chamber storage characteristics) • Enzyme activity retention rate (%, core enzyme activity retention of enzyme hydrolysate stored at 4℃ for 3 months); • pH fluctuation of the compounded system (pH difference before and after compounding, ≤0.5 is acceptable). • Separate compartment storage prevents layering / sedimentation / odor (visual and sensory evaluation).

[0080] 2. Core indicators of the finished product after compounding • Enzymatic hydrolysis efficiency: Total conversion efficiency of precursors (%), quercetin / kaempferol / myricetin content (mg / kg raw material), and utilization rate of active ingredients (%); • Anti-hair loss efficacy: Total inhibition rate of 5α-reductase (%), hair loss reduction rate in 28-day human trials (%), and hair density increase rate (%). • Scalp care benefits: TNF-α / IL-6 inhibition rate (%, anti-inflammatory), DPPH free radical scavenging rate (%, antioxidant), scalp transepidermal water loss (TEWL) improvement rate (%, barrier repair); • System compatibility: scalp irritation score (0-4 points, the lower the better), active ingredient retention rate of finished product after 6 months of room temperature storage (%), and no yellowing / stratification of the system (visual evaluation).

[0081] (III) Basic enzymatic hydrolysis process (adapted to compartment storage) The reaction conditions for malt extract / citrus fruit fermentation broth were adjusted to suit the specific requirements, while the storage process for the enzymatic hydrolysate was optimized to meet the needs for independent use in separate chambers. • Buffer system: Citric acid-sodium citrate buffer, pH 5.2 (fits the scalp microenvironment, adapts to the enzyme activity of the two raw materials, and facilitates stable storage in compartments). • Reaction temperature: 35-38℃; • Reaction time: 5 hours; • Add 2% propylene glycol and 0.05% disodium EDTA to stabilize the enzymatic hydrolysate system, then add citrate-sodium citrate buffer to adjust the pH to 5.2, dispense into the A chamber of the product (enzymatic hydrolysate chamber), seal and store in the dark; Compounding process: Before use, rapidly compound the enzymatic hydrolysate in cavity A (containing at least one of the following: arborvitae leaf extract, malt extract, and citrus fruit fermentation broth, propylene glycol, disodium EDTA, and citrate-sodium citrate buffer) with the product in cavity B (finished product formulation cavity) at a volume ratio of 1:4. Use immediately after compounding to extend the stability time of the active ingredients after compounding (addition / use should be completed within 2 hours).

[0082] (iv) Optimization of the content of Platycladus orientalis leaf extract by enzymatic hydrolysis of malt extract alone (with compartmentalized formulation) The content of Platycladus orientalis leaf extract was fixed at 45% (total mass of enzymatic hydrolysate). The addition of malt extract was set in gradients: 2%, 4%, 6%, 8%, 10%, and 12% (based on the total mass of enzymatic hydrolysate). The blank control group consisted of deionized water. Three replicates were set for each group. Separate enzymatic hydrolysates were prepared according to the basic enzymatic hydrolysis process (reaction temperature: 37℃; reaction time: 5h). After storage at 4℃ for 3 months, enzyme activity retention was measured. The core efficacy indicators were then measured after compounding. The results of the optimization experiment on the content of Platycladus orientalis leaf extract by enzymatic hydrolysis of malt extract alone are shown in Tables 8-9. Table 8. Results of the experiment on optimizing the content of Platycladus orientalis leaf extract by enzymatic hydrolysis of malt extract alone. Table 9. Results of the experiment on optimizing the content of Platycladus orientalis leaf extract by enzymatic hydrolysis of malt extract alone. As shown in Tables 8-9, the optimal addition amount of malt extract for enzymatic hydrolysis alone is 6%, with a content range of 4%-8%. Within this range, the enzyme activity retention rate of the hydrolysate after 3 months is ≥90.1%, there are no abnormalities in compartment storage, the pH fluctuation after compounding is ≤0.3, the precursor conversion efficiency is ≥58.3%, the quercetin content is ≥78.5mg / kg of raw material, the 5α-reductase inhibition rate is ≥59.6%, and the scalp irritation score is ≤0.3. This balances compartment storage stability, compound compatibility, and enzymatic hydrolysis efficacy. Adding an amount higher than 8% does not provide additional efficacy improvement and increases production costs and reduces compartment storage stability.

[0083] (v) Optimization of the content of Platycladus orientalis leaf extract by enzymatic hydrolysis of citrus fruit fermentation broth (with chamber adaptation) The content of Platycladus orientalis leaf extract was fixed at 45% (percentage of total mass of enzymatic hydrolysate). The addition of citrus fruit fermentation broth was set in gradients: 3%, 6%, 9%, 12%, 15%, and 18% (based on the total mass of the enzymatic hydrolysate, adapting the liquid fermentation broth to the characteristics of compartment filling). The control group consisted of deionized water. Three parallel samples were set for each group. Compartmentalized enzymatic hydrolysates were prepared according to the basic enzymatic hydrolysis process (reaction temperature: 36℃; reaction time: 5h). The compartment storage and compounding indicators were tested, with a focus on the effect of high fermentation broth addition on compartment pH and stability. The results of the optimization experiment on the content of Platycladus orientalis leaf extract in citrus fruit fermentation broth alone are shown in Tables 10-11. Table 10 Results of the experiment on optimization of the content of Platycladus orientalis leaf extract by enzymatic hydrolysis of citrus fruit fermentation broth alone Table 11 Results of the experiment on optimization of the content of Platycladus orientalis leaf extract by enzymatic hydrolysis of citrus fruit fermentation broth alone The experimental results in Tables 10 and 11 show that the optimal addition amount for the single enzymatic hydrolysis of citrus fruit fermentation broth is 9%, with a content range of 6%-12%. Within this range, the enzyme activity retention rate of the hydrolysate after 3 months is ≥91.0%, there are no abnormalities in compartment storage, the pH fluctuation after compounding is ≤0.3, the precursor conversion efficiency is ≥52.3%, the quercetin content is ≥68.5mg / kg of raw material, the 5α-reductase inhibition rate is ≥55.2%, and the scalp irritation score is ≤0.3. If the addition amount is less than 6%, the enzyme activity will be insufficient, and if it is more than 12%, it will easily lead to pH imbalance and yellowing / precipitation in compartment storage, affecting the user experience of compartment storage.

[0084] (vi) Optimization of enzymatic hydrolysis content in the compound of malt extract and citrus fruit fermentation broth (with compartmentalized formulation) Based on the optimal results for single raw materials, the arborvitae leaf extract was fixed at 45% (total mass percentage of the enzymatic hydrolysate). Using malt extract (A: 4%, 6%, 8%) and citrus fruit fermentation broth (B: 6%, 9%, 12%) as factors, an L9(3) model was designed. 2 Orthogonal experiment. The core evaluation index is the comprehensive score after compounding (weighted by precursor conversion efficiency, quercetin content, and 5α-reductase inhibition rate, with a maximum score of 100 points) + the cavity adaptation score (weighted by compounding pH fluctuation and system stability, with a maximum score of 50 points), for a total score of 150 points, to determine the optimal compounding ratio. The results of the optimization experiment of enzymatic hydrolysis content of malt extract + citrus fruit fermentation broth compound are shown in Table 12: Table 12 Results of the experiment on optimization of enzymatic hydrolysis content of malt extract + citrus fruit fermentation broth As shown in Table 12, the optimal ratio of malt extract to citrus fruit fermentation broth is 6% malt extract and 9% citrus fruit fermentation broth (45% Platycladus orientalis leaf extract, based on the total mass of the enzymatic hydrolysate). The ratio of malt extract to citrus fruit fermentation broth is 4%-8% to 6%-12%. Under this ratio, the enzyme activity retention rate of the hydrolysate after 3 months reaches 93.5%, there are no abnormalities in compartment storage, the pH fluctuation after compounding is only 0.2, and the compounding compatibility score is 49 points (out of 50). At the same time, a synergistic effect of enzyme activity is produced, the precursor conversion efficiency reaches 82.6%, the quercetin content reaches 118.5 mg / kg of raw material, the 5α-reductase inhibition rate reaches 82.5%, the compounding efficacy score is 90.3 points, and the total score is 139.3 points, which is much higher than that of single raw material enzymatic hydrolysis. The enzyme hydrolysis mechanisms are complementary (β-glucosidase from malt extract targets and hydrolyzes flavonoid glycosides, while pectinase from citrus fruit fermentation broth destroys the cell walls of Platycladus orientalis leaves), and the compartmentalized storage and compounding compatibility are excellent, with no risk of using unregistered enzyme preparations.

[0085] (vii) Comprehensive verification of the efficacy and performance of the optimal content compartmentalized enzymatic hydrolysate. Example 2 A hair loss prevention composition includes the following raw materials: plant extract (Thuja orientalis leaf extract), enzyme activator (malt extract), and a reconstitution system; said reconstitution system includes a moisturizing stabilizer, a chelating agent, a buffer system, and deionized water.

[0086] The method for preparing the anti-hair loss composition includes the following steps: The enzyme activator is mixed with the reconstitution system (the enzyme activator is mixed with deionized water, moisturizing stabilizer, and chelating agent, and the pH value is adjusted with a buffer system) to prepare an enzyme reconstitution system, which is then mixed with the plant extract. The enzymatic reaction is carried out at a temperature of 37°C and an enzymatic hydrolysis time of 5 hours to obtain the hair loss prevention composition.

[0087] The enzyme activator is provided in the form of enzyme powder. In the enzyme reconstitution system, the enzyme powder accounts for 6.0% of the total mass, propylene glycol accounts for 2.0%, and disodium EDTA accounts for 0.05%. The pH of the citrate-sodium citrate buffer is adjusted to 5.2, with the remainder being deionized water. The mass of the Platycladus orientalis leaf extract is 45% of the total mass of the enzyme reconstitution system.

[0088] Example 3 A hair loss prevention composition includes the following raw materials: plant extract (Thuja orientalis leaf extract), enzyme activator (citrus fruit fermentation broth), and a reconstitution system; said reconstitution system includes a moisturizing stabilizer, a chelating agent, a buffer system, and deionized water.

[0089] The method for preparing the anti-hair loss composition includes the following steps: The enzyme activator is mixed with the reconstitution system (the enzyme activator is mixed with deionized water, moisturizing stabilizer, and chelating agent, and the pH value is adjusted with a buffer system) to prepare an enzyme reconstitution system, which is then mixed with the plant extract. The enzymatic reaction is carried out at a temperature of 36°C and an enzymatic hydrolysis time of 5 hours to obtain the hair loss prevention composition.

[0090] The enzyme activator is provided in the form of enzyme powder. In the enzyme reconstitution system, the enzyme powder accounts for 9.0% of the total mass, propylene glycol accounts for 2.0%, and disodium EDTA accounts for 0.05%. The pH of the citrate-sodium citrate buffer is adjusted to 5.2, with the remainder being deionized water. The mass of the Platycladus orientalis leaf extract is 45% of the total mass of the enzyme reconstitution system.

[0091] Example 4 A hair loss prevention composition includes the following raw materials: plant extract (Thuja orientalis leaf extract), enzyme activator (composed of malt extract and citrus fruit fermentation broth), and a reconstitution system; said reconstitution system includes a moisturizing stabilizer, a chelating agent, a buffer system, and deionized water.

[0092] The method for preparing the anti-hair loss composition includes the following steps: The enzyme activator is mixed with the reconstitution system (the enzyme activator is mixed with deionized water, moisturizing stabilizer, and chelating agent, and the pH value is adjusted with a buffer system) to prepare an enzyme reconstitution system, which is then mixed with the plant extract. The enzymatic reaction is carried out at a temperature of 38°C and an enzymatic hydrolysis time of 5 hours to obtain the hair loss prevention composition.

[0093] The enzyme activator is provided in the form of enzyme powder. In the enzyme reconstitution system, the enzyme powder accounts for 15.0% of the total mass (6% malt extract and 9% citrus fruit fermentation broth), propylene glycol accounts for 2.0%, and disodium EDTA accounts for 0.05%. The pH of the citrate-sodium citrate buffer is adjusted to 5.2, with the remainder being deionized water. The arborvitae leaf extract accounts for 45% of the total mass of the enzyme reconstitution system.

[0094] Three optimal concentrations of enzymatic hydrolysates (i.e., the enzyme reconstitution systems of Examples 2, 3, and 4) were selected: 6% malt extract, 9% citrus fruit fermentation broth, and a 6%+9% compound solution. Deionized water was used as a blank control (blank group), and the enzyme reconstitution system of Example 1 was used as a positive control. The stability of the compartment storage, in vitro physicochemical experiments after compounding, and human clinical trials were carried out to comprehensively verify the compatibility of the compartments, the enzymatic hydrolysis effect, the anti-hair loss effect, and the scalp care effect.

[0095] Verification of the stability and compatibility of compartmentalized storage: Each group of enzyme hydrolysates (enzyme reconstitution systems of Examples 1-4) were dispensed into cavity A of the product and stored at 4℃, 25℃, and 45℃ for 3 months, respectively. The enzyme activity retention rate, system appearance, and pH value were tested. After reconstitution, the changes in pH and active ingredient content of the system were monitored within 2 hours to verify the compatibility of the reconstitution.

[0096] The experimental results of cavity storage stability and compatibility verification of Examples 1-4 and the blank group are shown in Table 13 below: Table 13 Results of experiments verifying the stability and compatibility of compartmentalized storage in Examples 1-4 and the blank group As shown in Table 13, the enzyme activity retention rate of all experimental compartment enzymatic hydrolysates was ≥88.6% after 3 months of storage at 4℃ / 25℃ and ≥75.3% after storage at 45℃. There was no stratification / precipitation / odor after compartment storage, which meets the storage requirements for compartmentalized cosmetic products. The 6%+9% compounding group showed the best compatibility, with a pH difference of only 0.2 before and after compounding. The active ingredient retention rate reached 96.5% after 2 hours of compounding, which is comparable to the compatibility of the pure enzyme preparation in Example 1 with compartments. Moreover, it does not have the risk of unregistered enzyme preparations and is fully compatible with compartment-type product forms.

[0097] Validation of active ingredient release efficiency and raw material utilization (after compounding): The samples from Examples 2-4 were tested within 2 hours after compounding. The contents of quercetin, kaempferol, and myricetin in the extracts of Platycladus orientalis leaves after enzymatic hydrolysis were detected by HPLC. The raw material utilization rate (content of active ingredient / total content of precursor) was calculated and compared with the blank group and the positive control group (Example 1).

[0098] The results of the verification of active ingredient release efficiency and raw material utilization rate in Examples 1-4 and the blank group are shown in Table 14: Table 14. Verification results of active ingredient release efficiency and raw material utilization rate in Examples 1-4 and the blank group. As shown in Table 14, the test results indicate that the enzymatic hydrolysis of malt extract and citrus fruit fermentation broth alone can significantly improve the release efficiency of active substances from Platycladus orientalis leaf extract. The raw material utilization rate is more than 10 times higher than that of the blank group, approaching 80%-90% of that of the pure enzyme preparation in Example 1. The compound group showed the best enzymatic hydrolysis effect, with a precursor conversion efficiency of 82.6% and a raw material utilization rate of 78.5%, which was only slightly lower than the positive control group. Moreover, the compartment storage and compounding compatibility were excellent, meeting the needs of compartment product development in the cosmetic industry.

[0099] Core efficacy verification for hair loss prevention (after compounding) – In vitro experiment: 5α-reductase inhibition rate The inhibition rates of type I and II 5α-reductase in each group of samples (Examples 1-4) after compounding were detected by in vitro enzyme activity assay. The results are shown in Table 15 below: Table 15 In vitro experimental results of Examples 1-4 and the blank group As shown in Table 15, the three types of enzymatically digested samples can significantly inhibit 5α-reductase activity after being combined. The total inhibition rate of the combined group reached 82.5%, which is 2.9 times that of the blank group. It can effectively reduce the production of dihydrotestosterone (DHT) and inhibit hair follicle miniaturization.

[0100] Core efficacy verification for anti-hair loss (after compounding) – Human trial: Anti-hair loss effect in 28 days Ninety participants aged 25-45 with androgenetic alopecia (45 males and 45 females) were randomly divided into five groups (malt group, citrus group, compound group, blank group, and positive control group), with 18 participants in each group. The product was formulated as "Cavity A enzymatic hydrolysate (the anti-hair loss composition of Examples 1-4) + Cavity B finished product formula," and added to the base shampoo (6% by mass) after compounding. It was used once daily for 28 consecutive days. The hair loss reduction rate and hair density improvement rate were measured, and the results are shown in Table 16 below. Table 16 Results of human trials in Examples 1-4 and the control group As shown in Table 16, the hair loss reduction rate of the compound group reached 45.2% and the hair density increase rate reached 16.8% after 28 days, which is close to the anti-hair loss effect of the pure enzyme preparation in Example 1 (above 48%), and far superior to the blank group. Moreover, the anti-hair loss effect of male subjects was slightly higher than that of female subjects, which is consistent with the pathogenesis of androgenetic alopecia.

[0101] In addition, the compartmentalized form avoids long-term contact between the enzymatically hydrolyzed active ingredients and preservatives, surfactants and other ingredients in the finished product formula, further ensuring the stability of the active ingredients and that the efficacy does not decrease after compounding.

[0102] Anti-inflammatory and antioxidant (in vitro) efficacy verification: The inhibition rate of anti-inflammatory factors was detected using an LPS-induced RAW264.7 macrophage model, and antioxidant activity was detected using the DPPH / ABTS method. The results are shown in Table 17 below: Table 17. Results of anti-inflammatory and antioxidant efficacy verification in Examples 1-4 and the blank group. As shown in Table 17, the combined enzymatic hydrolysate has significant anti-inflammatory and antioxidant effects. The combined group showed a TNF-α inhibition rate of 78.5% and a DPPH scavenging rate of 85.3%, which are close to the anti-inflammatory and antioxidant effects of the pure enzyme preparation in Example 1.

[0103] Verification of scalp barrier repair efficacy (after compound formulation): Thirty participants with sensitive scalps and redness were randomly divided into three groups (malt group, citrus group, and compound group), with 10 participants in each group. The compounded products were formulated into a 1.0% (w / w) serum solution and used continuously for 14 days. Scalp TEWL values ​​and redness scores were measured, and the results are shown in Table 18 below. Table 18. Verification results of scalp barrier repair efficacy in Examples 2-4 As shown in Table 18, the anti-hair loss compositions of Examples 2-4 can effectively repair the scalp barrier. The TEWL improvement rate of the compound group reached 44.2%, and the scalp redness score was significantly reduced. The compartmentalized form did not affect the scalp care efficacy. Moreover, both raw materials are natural plant / fermentation-derived, mild and non-irritating, and suitable for sensitive scalps.

[0104] Stability test: The finished products from Examples 1-4 were subjected to accelerated stability tests at 4°C, 25°C, and 45°C for 6 months. The retention rate of active ingredients, appearance, pH, and viscosity were measured, and the results are shown in Table 19 below. Table 19 Stability test results of Examples 1-4 As shown in Table 19, the anti-hair loss compositions of Examples 1-4 have excellent compatibility with conventional cosmetic formulations after compounding. After 6 months of accelerated storage, the retention rate of active ingredients is ≥76.8%, and the compound group reaches 80.5%.

[0105] Safety experiment: A skin patch experiment was conducted on 20 healthy subjects. The anti-hair loss compositions of Examples 1-4 (10% mass concentration) were applied to the backs of the subjects. Skin reactions were observed after 48 hours. The results showed that no sensitization reactions such as redness, swelling, stinging, or itching occurred in any of the experimental groups. The scalp irritation score was ≤0.3 points, which met the requirements of the "Cosmetic Safety Technical Specifications (2022 Edition)". There was no risk of enzyme protein residue (natural raw materials do not have the protein sensitization problem of pure enzyme preparations).

[0106] Therefore, the compartmentalized form effectively avoids the long-term interaction between enzymatic active ingredients and the finished product formula, further improving the stability of the finished product. Moreover, the raw materials are natural, mild and non-irritating, with no risk of allergies or residues, meeting the safety and industrial production requirements of compartmentalized cosmetic products.

[0107] III. The enzyme activator consists of β-glucosidase derived from Aspergillus niger, malt extract, and citrus fruit fermentation broth; the plant extract is Platycladus orientalis leaf extract. Example 5 A hair loss prevention composition includes the following raw materials: plant extract (Thuja orientalis leaf extract), enzyme activator (composed of β-glucosidase derived from Aspergillus niger, malt extract and citrus fruit fermentation broth), and a reconstitution system; said reconstitution system includes a moisturizing stabilizer, a chelating agent, a buffer system and deionized water.

[0108] The method for preparing the anti-hair loss composition includes the following steps: The enzyme activator is mixed with the reconstitution system (the enzyme activator is mixed with deionized water, moisturizing stabilizer, and chelating agent, and the pH value is adjusted with a buffer system) to prepare an enzyme reconstitution system, which is then mixed with the plant extract. The enzymatic reaction is carried out at a temperature of 38°C and an enzymatic hydrolysis time of 5 hours to obtain the hair loss prevention composition.

[0109] The enzyme activator is provided in the form of enzyme powder. In the enzyme reconstitution system, the enzyme powder accounts for 17.0% of the total mass (2% β-glucosidase from Aspergillus niger, 6% malt extract, and 9% citrus fruit fermentation broth), propylene glycol accounts for 2.0%, and disodium EDTA accounts for 0.05%. The pH of the citrate-sodium citrate buffer is adjusted to 5.2, with the remainder being deionized water. The arborvitae leaf extract accounts for 45% of the total mass of the enzyme reconstitution system.

[0110] Performance tests were conducted on Example 5, and the results are shown in Table 20 below: Table 20 Performance test results of Example 5 IV. Using almond-derived β-glucosidase, cellulase complex, or their complex to replace β-glucosidase from Aspergillus niger, and combining it with malt extract and citrus fruit fermentation broth as an enzyme activator. Based on the identification of β-glucosidase from Aspergillus niger as the core enzyme, in order to broaden the range of raw material selection and improve process adaptability, we further verified that three forms of β-glucosidase from almonds, cellulase complex enzyme, and β-glucosidase + cellulase complex enzyme from almonds can all replace β-glucosidase from Aspergillus niger and can be combined with malt extract and citrus fruit fermentation broth to construct a highly efficient enzymatic reaction system.

[0111] In this system, the amount of Platycladus orientalis leaf extract added was 45% (mass percentage), the amount of malt extract added was 6%, the amount of citrus fruit fermentation broth added was 9%, the enzymatic hydrolysis temperature was 38~40℃, and the enzymatic hydrolysis time was 5h.

[0112] Example 6 A hair loss prevention composition comprising the following ingredients: plant extract (Platycladus orientalis leaf extract), 0.01 mmol / L Mn 2+ (Activator), enzyme activator (composed of almond-derived β-glucosidase, malt extract and citrus fruit fermentation broth) and reconstitution system; the reconstitution system includes moisturizing stabilizer, chelating agent, buffer system and deionized water.

[0113] The method for preparing the anti-hair loss composition includes the following steps: The enzyme activator was mixed with the reconstitution system (the enzyme activator was mixed with deionized water, a moisturizing stabilizer, and a chelating agent, and the pH was adjusted with a buffer system) to prepare an enzyme reconstitution system, which was then mixed with the 0.01 mmol / L Mn. 2+ The activator and plant extracts are mixed and subjected to an enzymatic reaction at pH 5.2-5.6, temperature 40°C, and enzymatic hydrolysis time of 5 hours to obtain the hair loss prevention composition.

[0114] The enzyme activator is provided in the form of enzyme powder. In the enzyme reconstitution system, the enzyme powder accounts for 15.2% of the total mass (0.2% almond-derived β-glucosidase, 6% malt extract, and 9% citrus fruit fermentation broth), propylene glycol accounts for 2.0%, and disodium EDTA accounts for 0.05%. The pH of the citrate-sodium citrate buffer is adjusted to 5.2, with the remainder being deionized water. The arborvitae leaf extract accounts for 45% of the total mass of the enzyme reconstitution system.

[0115] Example 7 A hair loss prevention composition includes the following raw materials: plant extract (Thuja orientalis leaf extract), enzyme activator (composed of cellulase complex enzyme, malt extract and citrus fruit fermentation broth), and a reconstitution system; said reconstitution system includes a moisturizing stabilizer, a chelating agent, a buffer system and deionized water.

[0116] The method for preparing the anti-hair loss composition includes the following steps: The enzyme activator is mixed with the reconstitution system (the enzyme activator is mixed with deionized water, moisturizing stabilizer, and chelating agent, and the pH value is adjusted with a buffer system) to prepare an enzyme reconstitution system, which is then mixed with the plant extract. The enzymatic reaction is carried out under the conditions of pH 4.8~5.3, temperature 42℃, and enzymatic hydrolysis time of 5 hours to obtain the anti-hair loss composition.

[0117] The enzyme activator is provided in the form of enzyme powder. In the enzyme reconstitution system, the enzyme powder accounts for 15.4% of the total mass (0.4% cellulase complex, 6% malt extract, and 9% citrus fruit fermentation broth), propylene glycol accounts for 2.0%, and disodium EDTA accounts for 0.05%. The pH of the citrate-sodium citrate buffer is adjusted to 5.2, with the remainder being deionized water. The arborvitae leaf extract accounts for 45% of the total mass of the enzyme reconstitution system.

[0118] Example 8 A hair loss prevention composition comprising the following ingredients: plant extract (Platycladus orientalis leaf extract), 0.01 mmol / L Mn2+ (Activator), enzyme activator (composed of almond-derived β-glucosidase, cellulase complex enzyme, malt extract and citrus fruit fermentation broth) and reconstitution system; the reconstitution system includes moisturizing stabilizer, chelating agent, buffer system and deionized water.

[0119] The method for preparing the anti-hair loss composition includes the following steps: The enzyme activator was mixed with the reconstitution system (the enzyme activator was mixed with deionized water, a moisturizing stabilizer, and a chelating agent, and the pH was adjusted with a buffer system) to prepare an enzyme reconstitution system, which was then mixed with the 0.01 mmol / L Mn. 2+ The activator and plant extracts are mixed and subjected to an enzymatic reaction at pH 5.0-5.4, temperature 40℃, and enzymatic hydrolysis time of 5 hours to obtain the hair loss prevention composition.

[0120] The enzyme activator is provided in the form of enzyme powder. In the enzyme reconstitution system, the enzyme powder accounts for 15.3% of the total mass (0.10% almond-derived β-glucosidase, 0.20% cellulase complex, 6% malt extract, and 9% citrus fruit fermentation broth), propylene glycol accounts for 2.0%, and disodium EDTA accounts for 0.05%. The pH of the citrate-sodium citrate buffer is adjusted to 5.2, with the remainder being deionized water. The arborvitae leaf extract accounts for 45% of the total mass of the enzyme reconstitution system.

[0121] Performance tests were conducted on Examples 6-8, and the test results are shown in Table 21 below: Table 21 Performance test results of Examples 6-8 The test results in Table 21 show that: 1. Almond-derived β-glucosidase, cellulase complex enzyme, and a combination of both can effectively replace β-glucosidase from Aspergillus niger and form a stable enzymatic reaction system when combined with malt extract and citrus fruit fermentation broth. 2. The total precursor conversion efficiency of the three compound systems is ≥82%, which is much higher than the conversion efficiency of human endogenous enzymes (≤25%), and can efficiently convert inactive glycoside precursors in Platycladus orientalis leaves into free flavonoid active ingredients; 3. The β-glucosidase + cellulase complex enzyme group derived from almonds showed synergistic effects, with a higher conversion efficiency than the single cellulase complex enzyme group, but slightly lower than the single almond enzyme group, and the process was highly flexible. 4. All three alternative systems can be used to prepare anti-hair loss cosmetics, with controllable safety and compatibility that meets the requirements of cosmetic formulations.

[0122] V. The enzyme activator is β-glucosidase derived from Aspergillus niger, and the plant extracts are Platycladus orientalis leaf extract, Ligustrum lucidum extract, and Polygonum multiflorum extract. (1) Coordination system of plant extracts containing glycoside precursors obtained by targeted enzymatic hydrolysis Based on the above-mentioned optimal enzymatic hydrolysis conditions (pH 5.0-5.5, temperature 25-40℃, enzyme addition 0.2%, hydrolysis time 4h, citric acid-sodium citrate as buffer system, and propylene glycol as moisturizing stabilizer), this invention focuses on commercially available extracts of Platycladus orientalis leaf, Ligustrum lucidum, and Polygonum multiflorum as the core research objects. It does not involve the extraction and purification processes of the extracts themselves, but focuses on the precise optimization of the compound ratio of the three in the enzymatic hydrolysis system. At the same time, it emphasizes the universal advantages of directional enzymatic hydrolysis technology, breaks through the limitations of single substrate application, and provides technical support for the subsequent expansion of multi-component synergistic anti-hair loss systems. It should be noted that the directional enzymatic hydrolysis technology used in this invention is not limited to a single substrate such as Platycladus orientalis leaf extract. Its core enzyme, Aspergillus niger β-glucosidase (enzyme activity ≥1000U / g), has highly specific catalytic activity for flavonol glycoside structures. This specificity stems from the precise matching between the active site of the enzyme molecule and the flavonol glycoside nucleus—selectively hydrolyzing only the glycosidic bonds such as glucosyl and rhamnosyl linked at the C3 and C7 positions, without destroying the flavonol nucleus structure and other active functional groups, ensuring that the anti-desorption activity of the hydrolysis product is not affected.

[0123] (2) Promote the coordinated system of targeted enzymatic hydrolysis of plant extracts Based on the aforementioned optimal enzymatic hydrolysis process conditions, this invention focuses on the precise optimization of the compound ratio of commercially available extracts of Platycladus orientalis leaves, Ligustrum lucidum, and Polygonum multiflorum. This aims to leverage the efficient release of targeted anti-hair loss active ingredients from enzymatically hydrolyzed Platycladus orientalis leaves as a core premise, and further enhance the core anti-hair loss efficacy by combining it with Ligustrum lucidum and Polygonum multiflorum extracts. This expands the scalp care and other multi-dimensional additional effects, forming a synergistic system of "enzymatic transformation + compound enhancement." The selection of these three extracts as the core combination, rather than other plant-derived ingredients, is the result of a comprehensive screening based on four dimensions: compatibility with anti-hair loss mechanisms, synergistic efficacy, safety, and industrial suitability. This approach also avoids the limitations of existing technologies, such as the limited efficacy of single extracts and the lack of synergistic effects from unreasonable compounding. The specific selection logic and reasons for excluding competitors are as follows: Firstly, the arborvitae leaf extract serves as the core precursor source, an exclusive and irreplaceable substrate for the enzymatic hydrolysis system. As previously stated, the arborvitae leaf extract contains over 90% glycoside precursors such as quercetin and isoquercetin. Through the targeted enzymatic hydrolysis system of this invention, it can be efficiently converted into targeted anti-hair loss active ingredients such as quercetin and kaempferol, directly acting on the three core pathways of hair loss—inhibiting 5α-reductase activity, reducing scalp oxidative stress, and delaying hair follicle miniaturization. It is the fundamental core component of the enzymatic anti-hair loss system. Compared to other flavonoid plant extracts (such as ginkgo leaf and honeysuckle extracts), the content of glycoside precursors in arborvitae leaf extract is more than 30% higher, and it has the best specific compatibility with Aspergillus niger β-glucosidase, with a conversion efficiency more than 40% higher than that of ginkgo leaf extract. At the same time, arborvitae leaf extract has a long history of application in the cosmetics field. It has been certified by the "Cosmetic Safety Technical Specifications (2022 Edition)" and its safety is better than that of rare plant extracts such as photinia leaf and sage. It has no potential risk of sensitization or irritation. The raw material is commercially mature and has strong batch stability, which can meet the needs of industrial mass production.

[0124] Secondly, privet fruit extract can enhance the core anti-hair loss effect and compensate for the shortcomings of the enzymatic hydrolysate of arborvitae leaves. The core active ingredients of privet fruit extract are oleanolic acid and ursolic acid. These two not only synergistically enhance the 5α-reductase inhibition rate with the enzymatic hydrolysate of arborvitae leaves (quercetin, etc.), but also specifically activate the Wnt / β-catenin signaling pathway of hair follicle stem cells, prolonging the hair follicle growth phase. At the same time, they have significant anti-inflammatory activity, which can inhibit the release of scalp inflammatory factors (TNF-α, IL-6) and relieve scalp redness and itching associated with hair loss. Compared to anti-inflammatory plant extracts such as Sophora flavescens and Scutellaria baicalensis, Ligustrum lucidum extract and Platycladus orientalis leaf enzymatic hydrolysate exhibit stronger synergistic effects. The combined 5α-reductase inhibition rate is over 25% higher than that of a single ingredient alone, without any antagonistic effects. Its fat-soluble components also promote scalp penetration of water-soluble active ingredients such as quercetin, addressing the insufficient penetration of Platycladus orientalis leaf enzymatic hydrolysate alone—an advantage that water-soluble extracts like Sophora flavescens and Scutellaria baicalensis cannot achieve. Furthermore, the procurement cost of Ligustrum lucidum extract is only 1 / 5 that of niche anti-inflammatory plant extracts such as Smilax glabra, and its purity is easily controlled, making it suitable for industrial cost management needs.

[0125] Thirdly, Polygonum multiflorum extract focuses on protecting active ingredients and optimizing the scalp microenvironment, forming a closed loop of "conversion-strengthening-protection". The core active ingredients of Polygonum multiflorum extract are stilbene glycosides and emodin. Stilbene glycosides have an antioxidant capacity more than 5 times that of vitamin C, effectively scavenging reactive oxygen species (ROS) on the scalp and protecting the free flavonoids from oxidation after enzymatic hydrolysis of Platycladus orientalis leaves, thus extending the half-life of active ingredients. Emodin regulates scalp microcirculation, replenishes nutrients to hair follicles, and improves the hair follicle survival environment, complementing the hair follicle nourishing effects of Ligustrum lucidum extract. Compared to antioxidant plant extracts such as grape seed and tea polyphenols, the antioxidant components of Polygonum multiflorum extract have better compatibility with the enzymatic hydrolysis system (buffer solution, stabilizer), preventing adverse reactions. Furthermore, its specific effect of regulating scalp microcirculation is not found in grape seed extract. Simultaneously, the application scenarios of Polygonum multiflorum extract in hair care are more precise. Compared to general antioxidant ingredients, it avoids the dilution of anti-hair loss efficacy due to generalization, making it more targeted.

[0126] Fourth, the core reason for excluding other common anti-hair loss plant extracts is that they cannot meet the comprehensive requirements of "comprehensive mechanism coverage, excellent synergy, and compatibility with enzymatic hydrolysis systems." For example, although ginger extract can promote scalp blood circulation, its core component gingerol is highly irritating, easily causing scalp redness, and it inhibits β-glucosidase activity, reducing the conversion efficiency of precursors and conflicting with the enzymatic hydrolysis system; peppermint extract only provides a cooling sensation and has no substantial anti-hair loss activity, and its volatile components can also damage the stability of the complex stable system, leading to a shortened product shelf life; black sesame extract mainly nourishes hair and cannot act on the core mechanism of hair loss (5α-reductase inhibition, oxidative stress relief), thus failing to form a closed-loop effect; although grape seed and tea polyphenol extracts have strong antioxidant capabilities, they have poor compatibility with the enzymatic hydrolysis products of arborvitae leaves, easily react with buffer systems, and lack synergistic effects such as hair follicle nourishment and anti-inflammation, thus failing to construct a multi-dimensional anti-hair loss system. In addition, while niche extracts such as those from related plants of the Platycladus orientalis and Photinia serratifolia have some potential for preventing hair loss, they have low commercialization rates, high costs, poor batch stability, and their compatibility with the directional enzymatic hydrolysis system of this invention has not been verified, making it difficult to meet the needs of industrial mass production.

[0127] Based on the above selection logic, this invention uses L9(3) 3An orthogonal experiment was conducted, using "quercetin concentration after enzymatic hydrolysis, total 5α-reductase inhibition rate, active ingredient retention rate, and scalp irritation" as core evaluation indicators. "Anti-inflammatory activity and scalp microecological regulation ability" were also added as auxiliary indicators. The optimal compound ratio of Platycladus orientalis leaf extract: Ligustrum lucidum extract: Polygonum multiflorum extract = 10:2:1 (mass ratio) was selected to achieve a synergistic closed loop of "precursor supply - efficacy enhancement - activity protection - multidimensional synergistic effect." To ensure the consistency, reliability, and stability of the compounding experiment and industrial application, the commercial specifications of each extract were strictly limited, based on the principles of "efficacy onset threshold, safety, and cost controllability," as detailed below: • Platycladus orientalis leaf extract: Commercially available powder, with a total content of quercetin, isoquercetin, afotiam, and myricetin ≥15% (ensuring sufficient supply of precursors to meet the conversion requirements of the targeted enzymatic hydrolysis system and ensure that the yield of active ingredients meets the standards), solids ≥30%, moisture ≤8%, no pesticide residues (complies with GB / T 35956-2023 "General Technical Requirements for Plant Extracts"), pH value 4.5-6.5, ash content ≤5%, avoiding interference with enzymatic hydrolysis efficiency due to moisture absorption and clumping, excessive impurities, or abnormal pH, while ensuring the stability of raw material storage.

[0128] • Privet fruit extract: Commercially available powder (or fluid extract, solids ≥60%), oleanolic acid content ≥5% (ensuring its inhibitory effect on type II 5α-reductase reaches the effective threshold, forming a synergistic inhibitory effect with the enzymatic hydrolysis product of Platycladus orientalis leaves), moisture ≤8%, heavy metals ≤10ppm (as Pb), arsenic ≤2ppm, mercury ≤0.1ppm, odorless and free of visible impurities, meeting the safety requirements of plant raw materials in the "Cosmetic Safety Technical Specifications (2022 Edition)", avoiding the risk of scalp irritation caused by excessive heavy metals or impurities, suitable for people with sensitive scalps.

[0129] Polygonum multiflorum extract: Commercially available freeze-dried product with stilbene glycoside content ≥2% (meeting the antioxidant protection requirements for free flavonoids after enzymatic hydrolysis, extending the half-life of active ingredients), heavy metals ≤10ppm (as Pb), relative density 1.18-1.22 (25℃, in fluid extract state), antioxidant component (stilbene glycoside + emodin) retention rate ≥90%, moisture ≤5%, solubility ≥95% (5% by mass in deionized water), ensuring rapid and uniform dispersion in the enzymatic hydrolysis system without precipitation, while enhancing the protection effect of active ingredients, suitable for industrial feeding and formulation mixing requirements. ① Extract specifications To ensure the consistency and reliability of the compounding experiments, the commercial specifications of each extract were limited, and were determined based on the principles of "efficacy onset threshold, safety, and cost controllability," as follows: Platycladus orientalis leaf extract: commercially available powder, with a total content of quercetin, isoquercetin, afotiam, and myricetin ≥15% (ensuring sufficient precursors to meet enzymatic hydrolysis requirements), solids ≥30%, moisture ≤8%, and no pesticide residues (compliant with GB / T 35956-2023), avoiding moisture absorption, clumping, or impurities interfering with enzymatic hydrolysis.

[0130] Privet fruit extract: a commercially available extract with oleanolic acid content ≥5% (ensuring type II 5α-reductase inhibition efficacy), moisture ≤8%, heavy metals ≤10ppm (as Pb), free of odor and impurities, in compliance with the "Cosmetic Safety Technical Specifications (2022 Edition)" to avoid irritation risks.

[0131] Polygonum multiflorum extract: commercially available freeze-dried product with stilbene glycoside content ≥2% (meeting antioxidant protection requirements), heavy metals ≤10ppm (as Pb), relative density 1.18-1.22 (25℃), antioxidant component retention rate ≥90%, ensuring easy dispersion in enzymatic hydrolysis system and strengthening the protection of active ingredients.

[0132] ② Optimization experiment of compound ratio (with the above enzymatic hydrolysis conditions fixed) Experimental design: Using Platycladus orientalis leaf extract (A), Ligustrum lucidum extract (B), and Polygonum multiflorum extract (C) as factors, three addition levels were set for each (A: 2% / 3.5% / 5%, B: 0.5% / 0.75% / 1%, C: 0.3% / 0.55% / 0.8%), according to L9(3 3 An orthogonal design experiment was conducted, with each group treated using the above-mentioned optimal enzymatic hydrolysis process. Three parallel samples were set up, and the evaluation indicators and results are shown in Table 22 below: Table 22 Results of the Optimization Experiment of Compound Ratio Among them, experiment number 3 is Example 9.

[0133] Example 9 A hair loss prevention composition comprises the following raw materials: plant extracts (composed of Platycladus orientalis leaf extract, Ligustrum lucidum extract, and Polygonum multiflorum extract in a mass ratio of 10:2:1), an enzyme activator (β-glucosidase derived from Aspergillus niger), and a reconstitution system; the reconstitution system comprises a moisturizing stabilizer (propylene glycol), a chelating agent (disodium EDTA), a buffer system (citric acid-sodium citrate buffer), and deionized water. The mass content of the β-glucosidase derived from Aspergillus niger is 0.2%, and the mass content of the plant extracts is 4.55% (composed of 3.5% Platycladus orientalis leaf extract, 0.7% Ligustrum lucidum extract, and 0.35% Polygonum multiflorum extract).

[0134] The method for preparing the anti-hair loss composition includes the following steps: The enzyme activator is mixed with the reconstitution system (the enzyme activator is mixed with deionized water, moisturizing stabilizer, and chelating agent, and the pH value is adjusted with a buffer system) to prepare an enzyme reconstitution system, which is then mixed with the plant extract. The enzymatic reaction is carried out at a temperature of 38°C and an enzymatic hydrolysis time of 4 hours to obtain the hair loss prevention composition.

[0135] The enzyme activator is provided in the form of enzyme powder. In the enzyme reconstitution system, the enzyme powder accounts for 2.0% of the total mass, the propylene glycol accounts for 5.0% of the total mass, and the disodium EDTA accounts for 0.05% of the total mass. The pH of the citrate-sodium citrate buffer is adjusted to 5.2, and the remainder is deionized water.

[0136] ③ Synergistic mechanism of optimal compound system Under fixed enzymatic hydrolysis conditions, a compound ratio of Platycladus orientalis leaf extract, Ligustrum lucidum extract, and Polygonum multiflorum extract in a mass ratio of 10:2:1 can form a highly efficient synergistic closed loop. The extracts have complementary functions and no component antagonism. The specific mechanism and the effects of active ingredients are shown in Table 23 below: Table 23 Specific mechanisms and effects of active ingredients in the optimal compound system VI. Application of Anti-Hair Loss Composition – Anti-Hair Loss Essence (Scalp Oil Control Type) (1) The formula composition (by weight percentage) of the anti-hair loss essence (scalp oil control type) is shown in Table 23 below: Table 24. Formula composition (percentage by weight) of anti-hair loss serum (oil-controlling scalp type) The optimal addition amount in Table 24 is Experimental Example 1. The formula and preparation method of the anti-hair loss essence in Experimental Example 1 are shown below.

[0137] Experimental Example 1 A hair loss prevention serum, composed of the following ingredients by weight percentage: The anti-hair loss composition of Example 9 contains 7.0% oil control agent, 1.5% penetration promoter, 4.0% moisturizing and soothing agent, 1.0% film-forming agent, 0.2% pH adjuster, 0.15% preservative, 0.7% stabilizer, 0.08% other additives, and the balance is deionized water.

[0138] The oil-controlling agent is betaine salicylate, the penetration enhancer is 1,3-propanediol, the moisturizing and soothing agent is panthenol (vitamin B5), the film-forming agent is sodium hyaluronate (molecular weight 100kDa), the pH adjuster is citric acid, the preservative is composed of phenoxyethanol and ethylhexylglycerin in a mass ratio of 1:1, the stabilizer is disodium EDTA, and the other auxiliary agents are fragrance (no essential oils / low allergens).

[0139] The preparation method of the aforementioned anti-hair loss essence includes the following steps: (1) The anti-hair loss composition was prepared according to the preparation method of the anti-hair loss composition in Example 9; (2) Aqueous phase preparation: Add deionized water to the mixing tank, heat to 48°C, add the penetration promoter, stabilizer and oil control agent in sequence, stir until completely dissolved, and keep warm for later use; (3) Addition of active ingredients: Cool down to 38°C, add the anti-hair loss composition, stir evenly at 900 r / min, keep warm for 10 min to ensure that the active ingredients are evenly dispersed and there is no local aggregation; (4) Adding moisturizing and film-forming system: Add moisturizing and soothing agent and film-forming agent, stir at a low speed of 500r / min for 15min to avoid the generation of bubbles and ensure that the film-forming agent is fully dissolved; (5) Fragrance and shaping: Adjust the pH to 5.2 with pH adjuster, add preservatives and other additives, stir evenly; filter through 0.22μm filter membrane (to remove impurities and air bubbles), aseptically fill, and quality inspect to obtain anti-hair loss essence.

[0140] This product incorporates a hair loss prevention composition (a compound system of Platycladus orientalis leaf extract, Ligustrum lucidum extract, and Polygonum multiflorum extract in a 10:2:1 ratio) under fixed enzymatic hydrolysis conditions. This composition activates glycoside precursors in Platycladus orientalis leaves through enzymatic hydrolysis, releasing core active ingredients such as quercetin and kaempferol. These ingredients form a synergistic closed loop with oleanolic acid and stilbene glycosides in Ligustrum lucidum and Polygonum multiflorum extracts, exhibiting complementary functions and no antagonistic effects. Employing an "oil-free formula + high penetration + targeted nourishment" system, it abandons the traditional high-oil, high-viscosity formula logic. Combined with lightweight penetrants and oil-controlling ingredients, it enables rapid penetration of active ingredients into hair follicles while avoiding sticky residue on the scalp, thus optimizing formula stability and active ingredient retention.

[0141] An experiment was conducted to screen the optimal dosage of the anti-hair loss serum composition (dual indicators of oil control and anti-hair loss). Six serum groups were prepared with the dosage of the anti-hair loss composition as the variable (0%, 3%, 5%, 7%, 10%, 12%), with three replicates in each group. The core evaluation indicators were 28-day hair loss reduction rate, scalp oil secretion (T-zone), scalp irritation, active ingredient retention rate, and scalp cleanliness. A 28-day human trial and accelerated stability test were conducted, and the results are shown in Table 25 below. Table 25 Results of the screening experiment for the optimal dosage of anti-hair loss essence composition. As shown in Table 25, when the amount of the anti-hair loss composition added is "7%", the anti-hair loss essence achieves the best dual effect of "oil control + anti-hair loss": the hair loss reduction rate in 28 days reached 48.6%, mainly due to the synergistic inhibition of type I and II 5α-reductase by quercetin and oleanolic acid in the anti-hair loss composition (total inhibition rate ≥91%); the sebum secretion in the T-zone of the scalp decreased by 41.0% compared with the blank group, which is attributed to the effect of quercetin in inhibiting the activity of sebaceous gland cells; the scalp freshness score was 5.0 (out of 5), the scalp irritation score was only 0.4, and after 3 months of storage, the retention rate of active ingredients such as quercetin and stilbene glycosides was 84.5% (thanks to the "double antioxidant protection network" formed by myricetin and stilbene glycosides). After 6 months of accelerated stabilization at 45℃, there was no turbidity, yellowing, or sticky residue on the scalp.

[0142] When the amount of anti-hair loss composition added is less than 5%, the concentration of active ingredients in the anti-hair loss composition does not reach the effective threshold, and the scalp oil control and anti-hair loss effects are significantly insufficient, with a decrease in sebum secretion of <30%; when the amount of anti-hair loss composition added is greater than 10%, the anti-hair loss effect is not significantly improved, and the high concentration of flavonoids leads to increased scalp irritation and decreased freshness, increasing production costs. Therefore, the optimal amount of anti-hair loss composition added is determined to be 7%.

[0143] A comparative experiment was conducted on the scalp oil control and anti-hair loss effects of the anti-hair loss essence in Experiment Example 1 (specifically for scalp): 60 subjects with oily scalps and hair loss were selected (30 men and 30 women, aged 20-40 years, with scalp sebum secretion ≥200μg / cm³). 2 •h), randomly divided into experimental group (the anti-hair loss essence of this invention, 7% anti-hair loss composition added), control group 1 (commercially available scalp anti-hair loss essence, containing 5% single unhydrolyzed Platycladus orientalis leaf extract), and control group 2 (traditional anti-hair loss essence, containing high viscosity thickener, 7% uncompounded Platycladus orientalis leaf hydrolysate), 20 people in each group, used once a day for 28 consecutive days, and the core indicators were tested. The experimental results are shown in Table 26 below: Table 26 Comparison of the anti-hair loss essence's scalp oil control and anti-hair loss effects in Experiment Example 1 As shown in Table 26, the scalp oil-controlling anti-hair loss essence of Experimental Example 1 of this invention has a 92% higher anti-hair loss efficacy than similar products on the market. The core reason is the synergistic effect of multiple active ingredients in the anti-hair loss composition (quercetin + oleanolic acid enhance enzyme inhibition, kaempferol enhances the binding force of hair follicle proteins, and stilbene glycoside prolongs the hair follicle growth phase); the amount of sebum secreted by the scalp is reduced by more than 40%, and the scalp is kept fresh and non-sticky throughout the process. The retention rate of active ingredients is more than 80%, which completely solves the technical defects of traditional anti-hair loss essences such as "insufficient oil control, easy loss of activity, and sticky scalp", and is suitable for the core needs of oily scalp anti-hair loss.

[0144] VII. Single-use pre-mixed anti-hair loss products Experimental Example 2 A single-use, pre-prepared anti-hair loss product comprises the ingredients of the anti-hair loss essence described in Example 1, including a dual-compartment packaging container. The dual-compartment packaging container physically isolates the enzyme activator from the remaining ingredients. The single-use, pre-prepared anti-hair loss product uses a single, easily foldable, double-compartment single-use bottle. The bottle is internally divided into a first compartment and a second compartment. The first compartment contains the remaining ingredients, and the second compartment contains the enzyme activator. A foldable connecting portion connects the first and second compartments.

[0145] (1) Product Structure Each single-use disposable scalp sanitary napkin features a double-compartment, easily foldable, and sealed structure. The total weight of each sanitary napkin is precisely controlled to 10g (suitable for a single scalp care application, preventing secondary contamination after opening). The material is an aluminum foil composite film (three-layer structure: outer PET + middle aluminum foil + inner PE, offering significantly superior light-blocking and oxygen-barrier performance compared to commercially available plastic disposable scalp sanitary napkins, with an oxygen permeability ≤0.5cm). 3 / (m 2 • 24h • 0.1MPa), moisture permeability ≤ 1.0g / (m 2 (24h) One compartment contains the enzyme powder system (0.67g), and the other contains the remaining ingredients of the anti-hair loss essence (9.33g). A pre-reserved crease connects the two compartments, with the breaking strength precisely controlled between 0.5-0.8N. This prevents leakage during transportation (no leakage after a 1.5m drop) and allows for easy breakage with smooth edges, eliminating the risk of scratches. Each pack contains 10 units and comes with an individual aluminum foil sealed bag, further enhancing storage stability, unlike other single-use disposables on the market that are only individually sealed and susceptible to moisture.

[0146] (2) Usage Break off the connecting part of the single-use ampoule and repeatedly squeeze both compartments to thoroughly mix the contents (mixing time is about 10 seconds, no additional stirring tool required). After mixing evenly, immediately apply to a clean scalp (it is recommended to use after blow-drying hair to avoid diluting the enzyme concentration and ensure enzymatic hydrolysis efficiency). Massage with your fingertips for 2 minutes to promote distribution (massage intensity 5-8N, consistent with daily massage habits, which can also promote the penetration of the essence into the hair follicle opening). No rinsing is required. The enzymatic hydrolysis reaction starts immediately after massage and completes saturation conversion within 4 hours, with an active ingredient scalp penetration rate of ≥98% (verified by the Franz diffusion pool method, using nude mouse back skin as the skin model, diffusion time 4 hours), reaching directly to the core area of ​​the hair follicle sheath. Suitable for nighttime care, business trips, and other portable scenarios, no complicated operation required.

[0147] (3) Performance data In the ready-to-use formulation mode, the stability of active ingredients is 2.6 times higher than that of pre-mixed serums on the market. After 6 months of storage at room temperature, the enzyme activity retention rate is 83% and the active ingredient retention rate is 82%, while the active ingredient retention rate of pre-mixed single-use serums on the market is ≤32%. After use, laser Doppler blood flow measurement shows that the scalp microcirculation blood flow velocity increases by ≥30%, and the nutrient supply to hair follicles is enhanced. Clinical verification (40 subjects with telogen effluvium, using one vial daily for 2 consecutive months) shows that the average hair density increases by 18% and the hair diameter increases by 12%, with no cases of scalp irritation or allergies (the reaction score of the skin patch test at 48 hours was 0), solving the problems of easy allergy and insignificant efficacy of single-use serums on the market.

[0148] (4) Advantages of industrialization Employing fully automated single-use filling and sealing equipment, it can achieve a production speed of 200 units / minute, consistent with the production efficiency of ordinary single-use serums, far exceeding the 100 units / minute efficiency of customized double-compartment single-use serums on the market; the easy-break connector is integrally molded using a mature process, with a pass rate of ≥99.5%; the packaging cost is comparable to that of ordinary single-use products, with a single unit cost increase of ≤0.3 yuan, enabling large-scale mass production and adapting to the conventional production line layout of beauty brands.

[0149] 8. Cyclodextrin-encapsulated trigger-type anti-hair loss products Experimental Example 3 A cyclodextrin-encapsulated trigger-type anti-hair loss product comprises the raw materials of the anti-hair loss essence of Test Example 1, wherein the enzyme activator in the raw materials is encapsulated by cyclodextrin to form microcapsule particles, and the microcapsule particles are dispersed in an essence base composed of the remaining raw materials.

[0150] Among them, the β-cyclodextrin microcapsules prepared by the saturated aqueous solution method innovatively encapsulate only the enzyme powder (lyophilized powder), while the precursor does not need to be encapsulated and is directly dispersed on the substrate, realizing the physical isolation and storage of the enzyme powder and the precursor. This avoids the stability risks of enzyme solution form and ensures that the enzyme and the precursor can quickly contact and react during use, which is different from the design mistakes of overall encapsulation in the market.

[0151] (1) Encapsulation process: β-cyclodextrin and deionized water are mixed at a ratio of 1:10 (w / v), heated to 50°C and stirred to dissolve (stirring speed 300r / min, precisely control the stirring intensity to avoid cyclodextrin molecule aggregation), enzyme powder is added (lyophilized powder is added directly without the need for organic solvent dissolution, eliminating solvent residue), stirred at constant temperature for 2h (to ensure that the enzyme powder fully enters the cyclodextrin cavity), cooled to room temperature and allowed to stand for 12h (to promote microcapsule formation), centrifuged (speed 3000r / min, time 10min), the precipitate is collected and vacuum dried (temperature 50°C, vacuum degree -0.09MPa, to avoid high temperature destroying enzyme activity) to obtain microcapsule particles. The optimized enzyme encapsulation rate is ≥92%, far exceeding the ≤80% encapsulation rate of similar products on the market; the microcapsule particle size is precisely controlled between 10-50μm (detected by a laser particle size analyzer, with uniform particle size distribution), ensuring a refreshing and particle-free feel on the skin and preventing clogging of hair follicles; the stability of the enzyme powder is further improved after encapsulation, with an enzyme activity retention rate of ≥78% after 12 months of storage at room temperature, which is significantly better than unencapsulated enzyme powder (enzyme activity retention rate ≤50% after 12 months of storage at room temperature) and encapsulated products on the market (≤65%).

[0152] (2) How to use: When using, the cyclodextrin coating layer is broken by rubbing the scalp with force (≥5N, which is consistent with the intensity of daily massage and does not require additional triggering conditions), releasing enzymes and precursors and initiating the enzymatic hydrolysis reaction. No additional mixing is required, making it suitable for lazy people's care and daily touch-up scenarios. Unlike products on the market that require temperature and pH triggering, the triggering conditions of this product are more in line with daily usage habits, requiring no special operation and making it more practical.

[0153] (3) Performance data: The conversion rate of active ingredients within 4 hours after kneading is ≥90%, far exceeding the ≤60% conversion efficiency of commercially available cyclodextrin-encapsulated products; the retention rate of active ingredients is ≥85% (pre-mixed group ≤30%); the enzyme activity retention rate after 12 months of storage at room temperature is ≥78%, significantly better than the unencapsulated group (≤22%) and the encapsulated group on the market (≤65%); the skin feels refreshing and non-sticky after application, and the scalp absorbs it quickly (completely forming a film within 5 minutes) without any residue accumulation; clinical verification (30 subjects, used 3 times a week for 3 consecutive months) showed that the hair loss reduction rate was ≥45%, which is comparable to the efficacy of dual-chamber shampoo and single-use essence, and the skin feel score (8.8 / 10) is higher than that of similar products on the market (≤7.0 / 10). At the same time, the microcapsule particles have good compatibility with various care product bases and can be adapted to various dosage forms such as essence, hair mask, and hair root nutrient solution, making the application scenarios wider.

[0154] (4) Industrial advantages: β-cyclodextrin is a commonly used raw material in cosmetics. It is inexpensive (about 50 yuan / kg, which is only 1 / 3 of the price of special capsule materials on the market). The encapsulation process is simple and does not require organic solvents. It can be achieved with existing microcapsule preparation equipment without the need for additional customized equipment. The microcapsule particles are compatible with various care product bases. There is no need to adjust the base formula. They can be directly added to the existing anti-hair loss product formula. The modification is simple and the cost is controllable. There are no harmful by-products in the production process. It meets the green production standards and is suitable for the large-scale mass production needs of the cosmetics industry. It has both economic and environmental benefits.

[0155] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in the present invention, and these modifications or substitutions should all be covered within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A hair loss prevention essence, characterized in that, The product comprises the following components by weight percentage: 5.0%~10.0% anti-hair loss composition; wherein the anti-hair loss composition comprises the following raw materials: plant extracts, enzyme activators, and a reconstitution system; the enzyme activator is selected from at least one of the following: β-glucosidase from Aspergillus niger, β-glucosidase from almonds, cellulase complex enzyme, malt extract, and citrus fruit fermentation broth; the plant extracts include Platycladus orientalis leaf extract; the reconstitution system comprises a moisturizing stabilizer, a chelating agent, a buffer system, and deionized water.

2. The anti-hair loss essence according to claim 1, characterized in that, It also includes the following components by weight percentage: oil control agent 1.0%~2.0%, penetration enhancer 3.0%~5.0%, moisturizing and soothing agent 0.5%~1.5%, film-forming agent 0.1%~0.3%, pH adjuster 0.1%~0.2%, preservative 0.5%~0.8%, stabilizer 0.05%~0.1%, and other additives 0.05%~0.15%.

3. The anti-hair loss essence according to claim 2, characterized in that, The oil-controlling agent is betaine salicylate, the penetration enhancer is 1,3-propanediol, the moisturizing and soothing agent is panthenol, the film-forming agent is sodium hyaluronate, the pH adjuster is citric acid or triethanolamine, the preservative is composed of phenoxyethanol and ethylhexylglycerin in a mass ratio of 1:1, the stabilizer is disodium EDTA, and the other auxiliary agents are fragrances.

4. The anti-hair loss essence according to claim 1, characterized in that, In the hair loss prevention composition, the plant extracts further include privet fruit extract and fleeceflower root extract, and the mass ratio of the arborvitae leaf extract, privet fruit extract and fleeceflower root extract is 10:2:

1.

5. The anti-hair loss essence according to claim 1, characterized in that, In the hair loss prevention composition, the enzyme activator is β-glucosidase derived from Aspergillus niger, and the mass ratio of the β-glucosidase derived from Aspergillus niger to the extract of Platycladus orientalis leaves is 1:(10-20).

6. The anti-hair loss essence according to claim 1, characterized in that, In the hair loss prevention composition, the enzyme activator is composed of malt extract and citrus fruit fermentation liquid; the weight ratio of the malt extract to the citrus fruit fermentation liquid is (4-8):(6-12).

7. The anti-hair loss essence according to claim 1, characterized in that, In the hair loss prevention composition, the enzyme activator is composed of β-glucosidase from Aspergillus niger, malt extract and citrus fruit fermentation broth, and the mass ratio of β-glucosidase from Aspergillus niger, malt extract and citrus fruit fermentation broth is 2:6:

9.

8. The anti-hair loss essence according to claim 1, characterized in that, In the hair loss prevention composition, the enzyme activator is composed of almond-derived β-glucosidase, malt extract and citrus fruit fermentation broth, and the mass ratio of almond-derived β-glucosidase, malt extract and citrus fruit fermentation broth is (0.10-0.20):(4-8):(6-12).

9. The anti-hair loss essence according to claim 1, characterized in that, In the hair loss prevention composition, the enzyme activator is composed of cellulase complex enzyme, malt extract and citrus fruit fermentation broth, and the mass ratio of cellulase complex enzyme, malt extract and citrus fruit fermentation broth is (0.20-0.40):(4-8):(6-12).

10. The anti-hair loss essence according to claim 1, characterized in that, In the hair loss prevention composition, the enzyme activator is composed of almond-derived β-glucosidase, cellulase complex enzyme, malt extract and citrus fruit fermentation broth, and the mass ratio of almond-derived β-glucosidase, cellulase complex enzyme, malt extract and citrus fruit fermentation broth is (0.10-0.20):(0.20-0.40):(4-8):(6-12).

11. The anti-hair loss essence according to claim 1, characterized in that, In the hair loss prevention composition, the moisturizing stabilizer is propylene glycol, the chelating agent is disodium EDTA, and the buffer system is citrate-sodium citrate buffer.

12. A method for preparing an anti-hair loss essence according to any one of claims 2-11, characterized in that, Includes the following steps: (1) Preparation of anti-hair loss composition: The enzyme activator is mixed with the reconstitution system to form an enzyme reconstitution system, and then mixed with the plant extract. The enzymatic reaction is carried out at a temperature of 25-42℃ and an enzymatic hydrolysis time of 4-7 hours to obtain the anti-hair loss composition. (2) Aqueous phase preparation: Add deionized water to the mixing tank, heat to 45~50℃, add the penetration promoter, stabilizer and oil control agent in sequence, stir until completely dissolved, and keep warm for later use; (3) Addition of functional components: Cool down to 35~40℃, add the anti-hair loss composition, stir evenly at a speed of 800~1000r / min, and keep warm for 10min; (4) Addition of moisturizing and film-forming system: Add moisturizing and soothing agent and film-forming agent, and stir at a low speed of 500r / min for 15min; (5) Fragrance and shaping: Adjust the pH to 5.0~5.5 with pH adjuster, add preservatives and other additives, stir evenly; filter through a 0.22μm filter membrane to obtain the anti-hair loss essence.

13. A single-use, pre-formulated anti-hair loss product, comprising the raw materials of the anti-hair loss essence as described in claim 1, characterized in that, The invention includes a double-compartment packaging container that physically isolates the enzyme activator in the raw materials from the remaining raw materials.

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