A feminine intimate antibacterial solution and its preparation method

Abstract

This invention discloses a feminine intimate antibacterial liquid and its preparation method, comprising plant active extracts, chlorhexidine acetate, and deionized water; the plant active extracts include a protective plant active extract, an antibacterial plant active extract, and a soothing plant active extract; the protective plant active extract is derived from Dendrobium officinale and Saposhnikovia divaricata, the antibacterial plant active extract is derived from Sophora flavescens, Phellodendron chinense, and Cnidium monnieri, and the soothing plant active extract is derived from Notopterygium incisum and Mentha haplocalyx. The beneficial effects of this invention are: it helps to improve the retention rate of active ingredients and prolong the antibacterial effect time; the resulting product has good stability, a refreshing feel without sticky residue, and the preparation process is mild, facilitating industrial production.

Description

Technical Field

[0001] This invention relates to the field of feminine hygiene technology, specifically to a feminine antibacterial solution and its preparation method. Background Technology

[0002] Currently, antibacterial products in the field of feminine hygiene mainly fall into two categories: chemical antibacterial products and plant-based antibacterial products. Chemical antibacterial products typically use chlorhexidine acetate and other similar antibacterial ingredients as their core antibacterial components. While they have a strong antibacterial effect, high concentrations of active ingredients can easily irritate the mucous membranes of the intimate area. Plant-based antibacterial products often use a few plants such as sophora flavescens and phellodendron amurense as their main ingredients. Although they are relatively mild, they usually suffer from a narrow antibacterial spectrum, insufficient antibacterial strength, low utilization rate of active ingredients, and a lack of mucosal protection.

[0003] In addition, existing products mostly use traditional preparation processes such as soaking and filtration, which usually do not systematically optimize temperature, extraction methods and retention of active ingredients, which can easily lead to the inactivation of heat-sensitive active ingredients, thus affecting the antibacterial timeliness and stability of the products. At the same time, some products have insufficient verification of the safety and long-term stability of intimate mucous membranes, making it difficult to simultaneously achieve high antibacterial properties, low irritation, film protection and user comfort.

[0004] Therefore, there is an urgent need to provide a feminine intimate antibacterial solution and its preparation method that can improve antibacterial effect, reduce irritation, and also have mucosal protection, long-term stability and a good user experience. Summary of the Invention

[0005] The purpose of this invention is to provide a feminine intimate antibacterial solution and its preparation method to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: In a first aspect, embodiments of the present invention provide a feminine intimate antibacterial solution, comprising plant active extracts, chlorhexidine acetate, and deionized water; The plant active extracts include film-protecting plant active extracts, antibacterial plant active extracts, and soothing plant active extracts; The protective plant active extract is derived from Dendrobium officinale and Saposhnikovia divaricata, the antibacterial plant active extract is derived from Sophora flavescens, Phellodendron chinense and Cnidium monnieri, and the soothing plant active extract is derived from Notopterygium incisum and Mentha haplocalyx.

[0007] Preferably, the plant active extract is 8%-20%, the chlorhexidine acetate is 0.5%-1.2%, and the remainder is deionized water.

[0008] Preferably, the plant active extract comprises 10%-15% by mass percentage, the chlorhexidine acetate comprises 0.7%-1.0%, and the balance is deionized water.

[0009] Preferably, the plant active extract is 11%-12%, the chlorhexidine acetate is 0.84%-0.88%, and the balance is deionized water.

[0010] Preferably, in the raw materials of the protective plant active extract, the mass ratio of Dendrobium officinale to Saposhnikovia divaricata is 1.5:1-2.5:1; in the raw materials of the antibacterial plant active extract, the mass ratio of Sophora flavescens, Phellodendron chinense and Cnidium monnieri is (2.5-3.5):(2.5-3.5):(1.5-2.5).

[0011] Preferably, the protective plant active extract is an extract obtained from Dendrobium officinale and Saposhnikovia divaricata through low-temperature enzymatic pretreatment, vacuum low-temperature concentration, low-frequency ultrasonic extraction and cold extraction purification; the antibacterial plant active extract is an extract obtained from Sophora flavescens, Phellodendron chinense and Cnidium monnieri through anaerobic fermentation, vacuum low-temperature concentration, dual-frequency ultrasonic extraction and cold extraction purification; and the soothing plant active extract is an extract obtained from Notopterygium incisum and Mentha haplocalyx.

[0012] Preferably, the antibacterial plant active extract contains small molecule active components with a molecular weight of less than 500 Da.

[0013] Secondly, embodiments of the present invention provide a method for preparing the female intimate antibacterial solution described in any of the first aspects above, comprising the following steps: S1. Prepare the enzymatic hydrolysis product of the protective film component and the fermentation product of the antibacterial component separately, and concentrate the two products under vacuum at low temperature. S2. The concentrated protective film component is extracted by low-frequency ultrasound, and the concentrated antibacterial component is extracted by dual-frequency ultrasound. Then, the purified solution is obtained by cold extraction. S3. Add Notopterygium incisum extract, peppermint extract, deionized water and chlorhexidine acetate to the purified solution, and mix to obtain a compound solution; S4. The compound solution is sterilized under ultra-high pressure and then aseptically filled to obtain the finished product of the female intimate antibacterial solution.

[0014] Preferably, in S1, the low-temperature enzymatic hydrolysis temperature is 25-35℃, the enzymatic hydrolysis time is 1h-3h, and the stirring speed is 40-60r / min; the anaerobic fermentation temperature is 30-40℃, and the fermentation time is 36-60h; the vacuum degree of the vacuum low-temperature concentration is -0.08 to -0.10MPa, and the concentration temperature is 40-50℃.

[0015] Preferably, the low-frequency ultrasonic frequency in S2 is 15-25kHz, and the dual-frequency ultrasonic frequency is 15-25kHz and 70-90kHz; the ultra-high pressure sterilization pressure in S4 is 200-500MPa.

[0016] Compared with the prior art, the beneficial effects of the present invention are: 1. This invention uses a low-concentration chlorhexidine acetate compounded with various plant active extracts, which can reduce the risk of mucosal irritation caused by chemical antibacterial ingredients while enhancing the antibacterial effect. The resulting antibacterial solution has a high antibacterial rate against Escherichia coli and Staphylococcus aureus, and a good antibacterial effect against Candida albicans. It has low vaginal irritation and is suitable for daily use by sensitive individuals and postpartum women.

[0017] 2. This invention utilizes the polysaccharide components in Dendrobium officinale and Saposhnikovia divaricata extracts to form a protective film on the mucous membrane surface of the intimate area, which helps maintain the mucous membrane barrier function and improves the situation where traditional antibacterial products only focus on antibacterial activity and lack mucous membrane maintenance, resulting in less discomfort after use.

[0018] 3. This invention employs a process combining low-temperature enzymatic hydrolysis, anaerobic fermentation, and dual-frequency ultrasonic extraction, which can reduce the inactivation of heat-sensitive active ingredients, improve the utilization rate of active ingredients, and extend the antibacterial effect time, thus meeting the needs of daily long-term care.

[0019] 4. This invention improves the stability of the product system through vacuum low-temperature concentration, cold extraction purification and ultra-high pressure sterilization processes. During a long storage period, the antibacterial rate decreases less, the active ingredient retention rate is high, the product is not prone to separation or deterioration, and the batch-to-batch differences are small.

[0020] 5. The preparation process of this invention is completed at a relatively low temperature, with low energy consumption, strong controllability of process steps, no large amount of organic solvents used, and environmentally friendly production process, making it suitable for large-scale industrial production. Attached Figure Description

[0021] Figure 1 This is a schematic diagram illustrating the preparation process of a feminine intimate antibacterial solution according to an embodiment of the present invention. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Unless otherwise specified, the raw materials, reagents, and equipment used in the following embodiments are commercially available; the methods used are conventional methods in the art. The Phellodendron bark, Sophora flavescens, Cnidium monnieri, Notopterygium incisum, Saposhnikovia divaricata, Dendrobium officinale, and Mentha haplocalyx used in the following embodiments preferably conform to the standards of the 2020 edition of the Chinese Pharmacopoeia; the purity of chlorhexidine acetate is preferably not less than 99.5%; and the cellulase is preferably food-grade cellulase. The equipment used may include enzymatic hydrolysis reactors, anaerobic fermentation tanks, vacuum low-temperature concentration tanks, low-frequency ultrasonic tanks, dual-frequency ultrasonic tanks, cold extraction purification machines, constant temperature compounding tanks, ultra-high pressure sterilization tanks, and aseptic filling machines. Example

[0024] This feminine intimate antibacterial solution is made from ingredients in the following proportions: by weight percentage, plant active extracts 11.14%, chlorhexidine acetate 0.86%, and deionized water to bring the total to 100%. The preparation method of the female intimate antibacterial solution is as follows: Step 1: Take Dendrobium officinale and Saposhnikovia divaricata in a mass ratio of 2:1, grind them to 80 mesh, add 10 times the total mass of deionized water and 0.3% cellulase, and enzymatically hydrolyze at 30℃ for 2 hours with a stirring speed of 50 r / min to obtain the enzymatic hydrolysate of the protective film component; transfer the enzymatic hydrolysate to a vacuum concentrator, concentrate it at a vacuum degree of -0.09MPa and a temperature of 45℃ to a solid content of 20%, then extract it by 20kHz low-frequency ultrasound at 30℃ for 30 minutes, and cold extract and purify it at 5℃ for 1 hour, and filter to obtain the active plant extract for the protective film; Step 2: Take Sophora flavescens, Phellodendron chinense and Cnidium monnieri in a mass ratio of 3:3:2, grind them to 80 mesh, add 10 times the total mass of deionized water, seal and purge with nitrogen to remove oxygen, and anaerobic ferment at 35℃ for 48h to obtain the antibacterial component fermentation broth; transfer the fermentation broth to a vacuum concentrator, concentrate it to a solid content of 20% at a vacuum degree of -0.09MPa and a temperature of 45℃, then extract it with 20kHz+80kHz dual-frequency ultrasound at 55℃ for 45min, and cold extract and purify it at 5℃ for 1h, and filter to obtain the antibacterial plant active extract; Step 3: Extract soothing plant active extracts from Notopterygium root and peppermint; mix the membrane-protecting plant active extract, antibacterial plant active extract, and soothing plant active extract to obtain plant active extracts; Step 4: Mix 11.14% of the plant active extract, 0.86% of chlorhexidine acetate, and the remaining deionized water to make up the mass fraction. Stir at 25°C and 80 r / min for 1.5 h to prepare the compound solution. The compound solution is then subjected to segmented ultra-high pressure sterilization at 200 MPa for 5 min and 500 MPa for 5 min. The solution is then filled in a sterile clean environment with a cleanliness level of 100,000 to obtain the female intimate antibacterial solution. Example

[0025] This feminine intimate antibacterial solution is made from the following ingredients in the specified proportions: by weight percentage, 10.0% plant active extracts, 0.70% chlorhexidine acetate, and deionized water to bring the total to 100%. The preparation method of the female intimate antibacterial solution is as follows: Step 1: Take Dendrobium officinale and Saposhnikovia divaricata in a mass ratio of 2:1, pulverize to 80 mesh, add 10 times the total mass of deionized water and 0.3% cellulase, and enzymatically hydrolyze at 30℃ for 2 hours with a stirring speed of 50 r / min to obtain the enzymatic hydrolysate of the protective film component; transfer the enzymatic hydrolysate to a vacuum concentrator, concentrate at a vacuum degree of -0.09MPa and a temperature of 45℃ to a solid content of 20%, then extract by 20kHz low-frequency ultrasound at 30℃ for 30 minutes, and cold extract and purify at 5℃ for 1 hour, and filter to obtain the active extract of the protective film plant; Step 2: Take Sophora flavescens, Phellodendron chinense and Cnidium monnieri in a mass ratio of 3:3:2, grind them to 80 mesh, add 10 times the total mass of deionized water, seal and purge with nitrogen to remove oxygen, and anaerobic ferment at 35℃ for 48h to obtain the antibacterial component fermentation broth; transfer the fermentation broth to a vacuum concentrator, concentrate it to a solid content of 20% at a vacuum degree of -0.09MPa and a temperature of 45℃, then extract it with 20kHz+80kHz dual-frequency ultrasound at 55℃ for 45min, and cold extract and purify it at 5℃ for 1h, and filter to obtain the antibacterial plant active extract; Step 3: Extract soothing plant active extracts from Notopterygium root and peppermint; mix the membrane-protecting plant active extract, antibacterial plant active extract, and soothing plant active extract to obtain plant active extracts; Step 4: Mix 10.0% of the plant active extract, 0.70% of chlorhexidine acetate, and the remaining deionized water to make up the mass fraction. Stir at 25°C and 80 r / min for 1.5 h to prepare the compound solution. Sterilize the compound solution in stages by maintaining 200 MPa for 5 min and 500 MPa for 5 min. Fill the solution in a sterile clean environment with a cleanliness level of 100,000 to obtain the female intimate antibacterial solution. Example

[0026] This feminine intimate antibacterial solution is made from ingredients in the following proportions: by weight percentage, plant active extracts 15.0%, chlorhexidine acetate 1.00%, and deionized water to bring the total to 100%. The preparation method of the female intimate antibacterial solution is as follows: Step 1: Take Dendrobium officinale and Saposhnikovia divaricata in a mass ratio of 2:1, pulverize to 80 mesh, add 10 times the total mass of deionized water and 0.3% cellulase, and enzymatically hydrolyze at 30℃ for 2 hours with a stirring speed of 50 r / min to obtain the enzymatic hydrolysate of the protective film component; transfer the enzymatic hydrolysate to a vacuum concentrator, concentrate at a vacuum degree of -0.09MPa and a temperature of 45℃ to a solid content of 20%, then extract by 20kHz low-frequency ultrasound at 30℃ for 30 minutes, and cold extract and purify at 5℃ for 1 hour, and filter to obtain the active extract of the protective film plant; Step 2: Take Sophora flavescens, Phellodendron chinense and Cnidium monnieri in a mass ratio of 3:3:2, grind them to 80 mesh, add 10 times the total mass of deionized water, seal and purge with nitrogen to remove oxygen, and anaerobic ferment at 35℃ for 48h to obtain the antibacterial component fermentation broth; transfer the fermentation broth to a vacuum concentrator, concentrate it to a solid content of 20% at a vacuum degree of -0.09MPa and a temperature of 45℃, then extract it with 20kHz+80kHz dual-frequency ultrasound at 55℃ for 45min, and cold extract and purify it at 5℃ for 1h, and filter to obtain the antibacterial plant active extract; Step 3: Extract soothing plant active extracts from Notopterygium root and peppermint; mix the membrane-protecting plant active extract, antibacterial plant active extract, and soothing plant active extract to obtain plant active extracts; Step 4: Mix 15.0% of the plant active extract, 1.00% of chlorhexidine acetate, and the remaining deionized water to make up the mass fraction. Stir at 25°C and 80 r / min for 1.5 h to form a compound solution. The compound solution is then subjected to segmented ultra-high pressure sterilization at 200 MPa for 5 min and 500 MPa for 5 min. The solution is then filled in a sterile clean environment with a cleanliness level of 100,000 to obtain a feminine intimate antibacterial solution. Example

[0027] This feminine intimate antibacterial solution is made from ingredients in the following proportions: by weight percentage, plant active extracts 11.14%, chlorhexidine acetate 0.86%, and deionized water to bring the total to 100%. The preparation method of the female intimate antibacterial solution is as follows: Step 1: Take Dendrobium officinale and Saposhnikovia divaricata in a mass ratio of 1.5:1, grind them to 80 mesh, add 10 times the total mass of deionized water and 0.3% cellulase, and enzymatically hydrolyze at 30℃ for 2 hours with a stirring speed of 50 r / min to obtain the enzymatic hydrolysate of the protective film component; transfer the enzymatic hydrolysate to a vacuum concentrator, concentrate it at a vacuum degree of -0.09MPa and a temperature of 45℃ to a solid content of 20%, then extract it by 20kHz low-frequency ultrasound at 30℃ for 30 minutes, and cold extract and purify it at 5℃ for 1 hour, and filter to obtain the active extract of the protective film plant; Step 2: Take Sophora flavescens, Phellodendron chinense, and Cnidium monnieri in a mass ratio of 2.5:3.5:1.5, pulverize them to 80 mesh, add 10 times the total mass of deionized water, seal and purge with nitrogen to remove oxygen, and anaerobic ferment at 35℃ for 48h to obtain the antibacterial component fermentation broth; transfer the fermentation broth to a vacuum concentrator, concentrate it at a vacuum degree of -0.09MPa and a temperature of 45℃ to a solid content of 20%, then extract it by dual-frequency ultrasound at 20kHz+80kHz at 55℃ for 45min, and cold extract and purify it at 5℃ for 1h, and filter to obtain the antibacterial plant active extract; Step 3: Extract soothing plant active extracts from Notopterygium root and peppermint; mix the membrane-protecting plant active extract, antibacterial plant active extract, and soothing plant active extract to obtain plant active extracts; Step 4: Mix 11.14% of the plant active extract, 0.86% of chlorhexidine acetate, and the remaining deionized water to make up the mass fraction. Stir at 25°C and 80 r / min for 1.5 h to prepare the compound solution. The compound solution is then subjected to segmented ultra-high pressure sterilization at 200 MPa for 5 min and 500 MPa for 5 min. The solution is then filled in a sterile clean environment with a cleanliness level of 100,000 to obtain the female intimate antibacterial solution.

[0028] Example 5

[0029] This feminine intimate antibacterial solution is made from ingredients in the following proportions: by weight percentage, plant active extracts 11.14%, chlorhexidine acetate 0.86%, and deionized water to bring the total to 100%. The preparation method of the female intimate antibacterial solution is as follows: Step 1: Take Dendrobium officinale and Saposhnikovia divaricata in a mass ratio of 2.5:1, pulverize to 80 mesh, add 10 times the total mass of deionized water and 0.3% cellulase, and enzymatically hydrolyze at 30℃ for 2 hours with a stirring speed of 50 r / min to obtain the enzymatic hydrolysate of the protective film component; transfer the enzymatic hydrolysate to a vacuum concentration tank, concentrate at a vacuum degree of -0.09MPa and a temperature of 45℃ to a solid content of 20%, then extract by 20kHz low-frequency ultrasound at 30℃ for 30 minutes, and cold extract and purify at 5℃ for 1 hour, and filter to obtain the active extract of the protective film plant; Step 2: Take Sophora flavescens, Phellodendron chinense, and Cnidium monnieri in a mass ratio of 3.5:2.5:2.5, pulverize them to 80 mesh, add 10 times the total mass of deionized water, seal and purge with nitrogen to remove oxygen, and anaerobic ferment at 35℃ for 48h to obtain the antibacterial component fermentation broth; transfer the fermentation broth to a vacuum concentrator, concentrate it to a solid content of 20% at a vacuum degree of -0.09MPa and a temperature of 45℃, then extract it with 20kHz+80kHz dual-frequency ultrasound at 55℃ for 45min, and cold extract and purify it at 5℃ for 1h, and filter to obtain the antibacterial plant active extract; Step 3: Extract soothing plant active extracts from Notopterygium root and peppermint; mix the membrane-protecting plant active extract, antibacterial plant active extract, and soothing plant active extract to obtain plant active extracts; Step 4: Mix 11.14% of the plant active extract, 0.86% of chlorhexidine acetate, and the remaining deionized water to make up the mass fraction. Stir at 25°C and 80 r / min for 1.5 h to prepare the compound solution. The compound solution is then subjected to segmented ultra-high pressure sterilization at 200 MPa for 5 min and 500 MPa for 5 min. The solution is then filled in a sterile clean environment with a cleanliness level of 100,000 to obtain the female intimate antibacterial solution.

[0030] Comparative Example 1 The only difference from Example 1 is that the mass percentage of chlorhexidine acetate is 1.2%; the other components, proportions and preparation steps are the same as in Example 1.

[0031] Comparative Example 2 The only difference from Example 1 is that: in S1, the low-temperature enzymatic hydrolysis and anaerobic fermentation are omitted, and room temperature soaking extraction is used; the remaining components, proportions and preparation steps are the same as in Example 1.

[0032] Comparative Example 3 The only difference from Example 1 is that: in S2, dual-frequency ultrasonic extraction is omitted and conventional stirring extraction is used; the remaining components, ratios and preparation steps are the same as in Example 1.

[0033] Comparative Example 4 The only difference from Example 1 is that S4 uses pasteurization instead of ultra-high pressure sterilization; the other components, proportions and preparation steps are the same as in Example 1.

[0034] Comparative Example 5 The only difference from Example 1 is that Dendrobium officinale and windproof protective film components are not added; the remaining components, proportions and preparation steps are the same as in Example 1.

[0035] Experimental Example 1 Test sample: Female intimate antibacterial solution prepared using the method of Example 1 of this invention.

[0036] Test basis: GB 15979-2002 Hygienic Standard for Disposable Sanitary Products, Disinfection Technical Specifications (2002 Edition), Cosmetic Safety Technical Specifications (2015 Edition), Enterprise Standard Q / 320111 YCX 002-2024.

[0037] 1. pH value measurement The test was conducted according to method 2.2.1.4 of the 2002 edition of the "Disinfection Technical Specifications". The results showed that the pH value of the test sample was 5.61, which is within the range of 5.0 to 7.0 and meets the relevant standard requirements.

[0038] 2. Determination of chlorhexidine acetate content and stability The determination was performed according to method 2.2.1.2.12 of the 2002 edition of the "Disinfection Technical Specifications". The results showed that the initial chlorhexidine acetate content of the test sample was 0.868%; after being placed at 37℃~40℃ and relative humidity ≥75% for 90 days, the chlorhexidine acetate content was 0.854%, with a content reduction rate of 1.61%, which is less than 10%. The sample has good stability, and the shelf life can be set at 2 years.

[0039] 3. Heavy metal determination Lead, arsenic, and mercury were determined according to the methods in the 2015 edition of the "Cosmetic Safety Technical Specifications". The results showed that the lead content of the test sample was <1.5 mg / kg, the arsenic content was <0.010 mg / kg, and the mercury content was 0.0051 mg / kg, all of which met the relevant standard limits.

[0040] 4. Determination of microbiological indicators The determination was conducted according to the methods in the appendix of GB 15979-2002 "Hygienic Standard for Disposable Sanitary Products". The results showed that the total bacterial count of the test samples was <1 cfu / mL, and the total fungal count met the standard requirements; coliform bacteria, Staphylococcus aureus, Pseudomonas aeruginosa, and hemolytic streptococci were not detected, meeting the relevant hygiene standards.

[0041] 5. Antibacterial performance test The determination was performed according to Appendix C4 of GB 15979-2002, using the suspension quantitative method, at a test temperature of 20℃±1℃. The results showed that after 5 min of contact with the original sample solution, the inhibition rate against *Escherichia coli* (8099), *Staphylococcus aureus* (ATCC6538), and *Candida albicans* (ATCC10231) all reached over 50%, demonstrating antibacterial activity. After 20 min of contact, the inhibition rate against *Escherichia coli* was 98.61%, against *Staphylococcus aureus* was 98.10%, and against *Candida albicans* was 96.34%.

[0042] 6. Vaginal mucosal irritation test The experiment was conducted according to method 2.3.5 of the 2002 edition of the "Disinfection Technical Specifications". The results showed that the vaginal mucosa of the rabbits in the test sample group was normal in morphology, without congestion or swelling, and the average score of the stimulation response was 0. According to the relevant grading standards, the sample was non-irritating to the vaginal mucosa and met the relevant standard requirements.

[0043] Experimental conclusions Under experimental conditions of 20℃±1℃, the average antibacterial rates of the stock solution of this invention against *Escherichia coli* (8099), *Staphylococcus aureus* (ATCC6538), and *Candida albicans* (ATCC10231) are shown in Table 1. As shown in Table 1, the stock solution of this invention, when acted on for 2 minutes, achieved an average antibacterial rate of over 88% against all three tested strains; after 20 minutes of action, the antibacterial rate against *Escherichia coli* was 98.61%, against *Staphylococcus aureus* was 98.10%, and against *Candida albicans* was 96.34%, demonstrating excellent antibacterial activity.

[0044] Table 1: Antibacterial effect of the stock solution of the present invention on different bacterial strains 2 90.79 91.23 88.45 5 93.58 94.17 91.72 10 95.68 96.32 94.08 20 98.61 98.10 96.34 Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A feminine intimate antibacterial solution, characterized in that, Includes plant active extracts, chlorhexidine acetate, and deionized water; The plant active extracts include film-protecting plant active extracts, antibacterial plant active extracts, and soothing plant active extracts; The protective plant active extract is derived from Dendrobium officinale and Saposhnikovia divaricata, the antibacterial plant active extract is derived from Sophora flavescens, Phellodendron chinense and Cnidium monnieri, and the soothing plant active extract is derived from Notopterygium incisum and Mentha haplocalyx.

2. The feminine intimate antibacterial solution according to claim 1, characterized in that, The plant active extract comprises 8%-20%, the chlorhexidine acetate comprises 0.5%-1.2%, and the remainder is deionized water.

3. The feminine intimate antibacterial solution according to claim 2, characterized in that, The plant active extract comprises 10%-15% by mass percentage, the chlorhexidine acetate comprises 0.7%-1.0%, and the balance is deionized water.

4. The feminine intimate antibacterial solution according to claim 3, characterized in that, The plant active extract comprises 11%-12% by mass percentage, the chlorhexidine acetate comprises 0.84%-0.88%, and the balance is deionized water.

5. The feminine intimate antibacterial solution according to any one of claims 1 to 4, characterized in that, In the raw materials of the protective plant active extract, the mass ratio of Dendrobium officinale to Saposhnikovia divaricata is 1.5:1-2.5:1; in the raw materials of the antibacterial plant active extract, the mass ratio of Sophora flavescens, Phellodendron chinense and Cnidium monnieri is (2.5-3.5):(2.5-3.5):(1.5-2.5).

6. The feminine intimate antibacterial solution according to any one of claims 1 to 5, characterized in that, The protective plant active extract is obtained from Dendrobium officinale and Saposhnikovia divaricata through low-temperature enzymatic hydrolysis pretreatment, vacuum low-temperature concentration, low-frequency ultrasonic extraction and cold extraction purification; the antibacterial plant active extract is obtained from Sophora flavescens, Phellodendron chinense and Cnidium monnieri through anaerobic fermentation, vacuum low-temperature concentration, dual-frequency ultrasonic extraction and cold extraction purification; the soothing plant active extract is obtained from Notopterygium incisum and Mentha haplocalyx.

7. The feminine intimate antibacterial solution according to claim 6, characterized in that, The antibacterial plant active extract contains small molecule active components with a molecular weight of less than 500 Da.

8. A method for preparing the female intimate antibacterial solution according to any one of claims 1 to 7, characterized in that, Includes the following steps: S1. Prepare the enzymatic hydrolysis product of the protective film component and the fermentation product of the antibacterial component separately, and concentrate the two products under vacuum at low temperature. S2. The concentrated protective film component is extracted by low-frequency ultrasound, and the concentrated antibacterial component is extracted by dual-frequency ultrasound. Then, the purified solution is obtained by cold extraction. S3. Add Notopterygium incisum extract, peppermint extract, deionized water and chlorhexidine acetate to the purified solution and mix to obtain a compound solution; S4. The compound solution is sterilized under ultra-high pressure and then aseptically filled to obtain the finished product of the female intimate antibacterial solution.

9. The preparation method according to claim 8, characterized in that, The low-temperature enzymatic hydrolysis temperature in S1 is 25-35℃, the hydrolysis time is 1h-3h, and the stirring speed is 40-60r / min; the anaerobic fermentation temperature is 30-40℃, and the fermentation time is 36-60h; the vacuum degree of vacuum low-temperature concentration is -0.08 to -0.10MPa, and the concentration temperature is 40-50℃.

10. The preparation method according to claim 8 or 9, characterized in that, The low-frequency ultrasonic frequency of S2 is 15-25kHz, and the dual-frequency ultrasonic frequency is 15-25kHz and 70-90kHz; the ultra-high pressure sterilization pressure of S4 is 200-500MPa.

Images