一种核桃粕抗菌肽及发酵法制备核桃粕抗菌肽的方法

By optimizing the preparation of walnut meal antimicrobial peptides through mixed-culture fermentation and separation technology, the problem of insufficient stability of antimicrobial peptides in high-temperature, wide-pH and high-salt ion environments has been solved, enabling efficient food preservation applications.

CN122404499APending Publication Date: 2026-07-17URUMQI SHANGSHANYUAN BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
URUMQI SHANGSHANYUAN BIOTECHNOLOGY CO LTD
Filing Date
2025-09-04
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies struggle to maintain the high efficiency and stability of antimicrobial peptides in high-temperature, wide-pH, and high-salt ion environments, limiting their application in food preservation.

Method used

Walnut meal antimicrobial peptides were prepared using a mixed-strain fermentation method. Strains such as Bacillus subtilis, Lactobacillus reuteri, Kluyveromyces marsupialis, Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus plantarum, and Bacillus natto were used. By optimizing fermentation conditions and combining ultrafiltration and column chromatography separation technology, walnut meal antimicrobial peptides with high antibacterial properties and environmental stability were prepared.

Benefits of technology

It improves the yield and antibacterial ability of antimicrobial peptides in walnut meal, and ensures that they maintain good structural stability and functional activity under a wide range of temperature, pH gradient, salt ion strength and protease action conditions, making them suitable for food preservation.

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Abstract

本发明提供了一种核桃粕抗菌肽及发酵法制备核桃粕抗菌肽的方法,所述核桃粕抗菌肽的氨基酸序列如SEQ ID NO:3或SEQ ID NO:4所示。本发明所述的核桃粕抗菌肽具有优异的抗菌稳定性,在宽泛的温度范围、pH梯度、盐离子强度及蛋白酶作用条件下,均表现出良好的结构稳定性和功能活性保留特性,本发明所述的发酵法制备核桃粕抗菌肽的方法使用特定组分的混菌发酵,并对发酵条件进行优化,提高了核桃粕抗菌肽的得率,改善了核桃粕抗菌肽的抑菌能力及稳定性。
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