一种有助于控制体内脂肪的PI蛋白及其制备方法和应用
By mutating and expressing potato PI protein, a high-thermal-stability mutant PI-6130-M14 was obtained, which solved the problem of insufficient thermal stability of PI protein and enabled its effective application under high-temperature conditions and significant weight loss effect.
CN122404537APending Publication Date: 2026-07-17YUNNAN NORMAL UNIV
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- YUNNAN NORMAL UNIV
- Filing Date
- 2026-06-18
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
The poor thermal stability of existing PI proteins limits their application at food processing temperatures, and existing obesity interventions have safety and applicability issues.
Method used
By rationally designing and site-directedly mutagenesis of multiple loop regions of potato PI protein, a mutant with high thermal stability, PI-6130-M14, was obtained and efficiently expressed in E. coli, thus reducing costs.
Benefits of technology
PI-6130-M14 showed little structural change at 70℃, maintained over 95% of its enzyme inhibitory activity, and significantly reduced body weight and visceral fat accumulation in obese mice on a high-fat diet, providing a safe and easily acceptable fat management solution.
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Figure CN122404537A_ABST
Abstract
本发明公开了一种有助于控制体内脂肪的PI蛋白及其制备方法和应用，属于生物医药及食品技术领域。本发明经过蛋白质改造技术获得了SEQ ID NO.1所示的具有高热稳定性的新型PI蛋白（PI‑6130‑M14），该蛋白在70℃处理下仍能保持其二级结构的完整性和胰蛋白酶抑制活性。经过大肠杆菌表达后产量可达567.9mg / L。动物实验结果表明，PI‑6130‑M14不仅能有效延缓肥胖小鼠体重增加，还能显著降低脂肪的过度积累，改善肝脏脂肪堆积的效果与阳性药物利拉鲁肽相当。本发明提供的新PI蛋白可作为活性成分，在制备减少脂肪积累并缓解肥胖的生物医药及食品保健等领域具有巨大的应用潜力。
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