一种草酸青霉工程菌及其构建方法
By constructing the engineered Penicillium oxalate strain QL-ΔCxrC-ΔCreA and knocking out both CxrC and CreA genes, the problem of insufficient release of expression potential in the existing Penicillium oxalate engineered strain was solved, and the amylase activity and protein expression levels were significantly improved, meeting the needs of high-end industrial applications.
CN122405451APending Publication Date: 2026-07-17HUNAN NORMAL UNIVERSITY
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HUNAN NORMAL UNIVERSITY
- Filing Date
- 2026-06-22
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing engineered Penicillium oxalate strains suffer from issues such as high background secretion, insufficient release of expression potential, and inadequate industrial adaptability, making it difficult to meet the industrial demands for high-expression, low-background, high-purity, and high-stability filamentous fungal expression systems.
Method used
We constructed an engineered Penicillium oxalate strain, QL-ΔCxrC-ΔCreA, and achieved synergistic regulation of dual targets by knocking out the CxrC and CreA genes, thereby increasing amylase production.
Benefits of technology
It significantly improved the activity of amylase and the content of extracellular protein, enhanced the activation efficiency of the promoter Pamy15A, and met the needs of high-end industries.
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Figure CN122405451A_ABST
Abstract
一种草酸青霉工程菌及其构建方法，该工程菌命名为草酸青霉(Penicillium oxalicum）QL‑ΔCxrC‑ΔCreA，其保藏编号为CCTCC NO：M 2026896。本发明通过敲除转录抑制因子CxrC和丝状真菌碳分解代谢阻遏的转录因子CreA得到草酸青霉工程菌，使得其在淀粉作为唯一碳源进行发酵时，其所产生的淀粉酶活性和胞外蛋白含量有了显著的提高；两个因子的敲除协同提高了启动子Pamy15A 的启动效率。
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