脂肪酶突变体及其在制备甘油二酯中的应用
By performing specific amino acid site mutations and high-throughput screening on lipase, the problem of low lipase activity was solved, resulting in a significant improvement in enzyme activity and catalytic efficiency, making it suitable for the preparation of diglycerides from various edible oils.
Patent Information
- Application Number
- CN202610873890.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-06-17
- Publication Date
- 2026-07-17
AI Technical Summary
Existing lipases suffer from low enzyme activity and poor catalytic efficiency in industrial applications, and immobilization treatment leads to severe loss of enzyme activity, making it difficult to meet industrial needs.
By mutating lipase, specific amino acid site mutations such as G60A, T62A, and Y67A were designed and heterologously expressed in E. coli. Combined with a high-throughput screening system, enzyme activity and catalytic efficiency were improved. The secretion efficiency of the enzyme was optimized by using secretion signal peptides and CRISPR/Cpf1 technology, and a high-throughput screening method based on clear zone and color change was established.
It significantly improves the specific enzyme activity and catalytic efficiency of lipase. The mutant has a 4.65-fold increase in specific enzyme activity and a 25.6% increase in secretion efficiency, enabling efficient preparation of diglycerides from oils and fats. It has wide applicability and is suitable for the hydrolysis of various edible oils.
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Figure CN122405591A_ABST
Abstract
Citation Information
Patent Citations
High-activity lipase mutant and application thereof in functional grease synthesis
CN120866274A