一种检测托拉氏假单胞菌Pseudomonas tolaasii的引物及应用
By designing specific primers to combine PCR and agarose gel electrophoresis, the time-consuming and accuracy problems of Pseudomonas tora detection have been solved, realizing a rapid and simple detection method suitable for detection applications in multiple scenarios.
Patent Information
- Application Number
- CN202610874133.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-06-17
- Publication Date
- 2026-07-17
AI Technical Summary
Existing detection methods for Pseudomonas tora are cumbersome, time-consuming, and susceptible to contamination by other bacteria, failing to meet the needs of rapid screening and emergency testing. Furthermore, traditional 16S rDNA sequencing cannot accurately identify species within the Pseudomonas genus.
By designing highly specific primers and combining them with PCR and agarose gel electrophoresis, DNA can be directly extracted from samples for amplification and electrophoretic detection, eliminating the need for separation, culture, and biochemical identification steps, thus enabling rapid detection.
It enables rapid and accurate detection of Pseudomonas tora, with a short detection cycle and high efficiency. It is suitable for general laboratory operations, compatible with standardized mass production of detection kits, and widely used in environmental monitoring and pathogen epidemiological investigations.
Smart Images
Figure CN122405862A_ABST