一种基于多重RT-PCR-反向点杂交技术检测多种猪呼吸道病原体的引物探针组合、试剂盒及应用
By using multiplex RT-PCR-reverse dot hybridization technology, combined with specific primers and probes, the problems of long detection time and high cost in existing technologies for detecting various porcine respiratory pathogens have been solved, achieving rapid detection results with high throughput, low cost, and good specificity.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SOUTH CHINA AGRICULTURAL UNIVERSITY
- Filing Date
- 2026-06-12
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies are insufficient for the efficient and low-cost simultaneous detection of multiple swine respiratory pathogens, especially for differential diagnosis in cases of mixed infections. Furthermore, conventional methods are time-consuming, costly, and lack specificity.
Using multiplex RT-PCR-reverse dot hybridization technology, combined with specific primers and probes, the reverse dot hybridization detection was performed on a nylon membrane after multiplex RT-PCR amplification, enabling the simultaneous detection of 11 porcine respiratory pathogens.
It enables rapid detection of multiple porcine respiratory pathogens with high throughput, low cost, and good specificity, reducing detection time and workload, and improving detection accuracy and sensitivity.
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Figure CN122405908A_ABST