一种栓皮栎叶片气孔观察样品的制备方法
By employing steps such as standard leaf selection, vacuum-assisted fixation, and epidermal hair removal under microscopic monitoring, the problems of sample differences and uneven fixation in the preparation of samples for stomatal observation of Quercus variabilis leaves were solved, achieving clear visualization and high-definition imaging of stomatal structures and providing a reliable morphological basis.
Patent Information
- Application Number
- CN202610674011.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-05-15
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies are insufficient to effectively remove the interference of epidermal hairs on the leaves of *Quercus variabilis*, resulting in low-quality sample preparation for stomatal observation. Furthermore, scanning electron microscopy is time-consuming and requires expensive equipment.
The process involved selecting standard sample leaves and marking leaf positions, vacuum-assisted fixation, directional scraping of epidermal hairs under microscopic monitoring, mild alkaline digestion of the mesophyll, specific staining, and gradient dehydration to ensure the consistency of the physiological state of the samples and the clear visualization of the stomatal structure.
It achieves clear visualization and integrity of pore structure, ensures sample repeatability and high-definition imaging, solves the problems of sample variability and uneven fixation in traditional methods, and provides a reliable morphological basis.
Smart Images
Figure CN122409284A_ABST