一种用于浮游生物智能分析的光学成像方法、系统

By setting microscope system parameters and fluid flow control, combined with adaptive image processing and a lightweight model, the problem of low detection accuracy in phytoplankton imaging was solved, achieving efficient and automated algae identification and population analysis.

CN122409436APending Publication Date: 2026-07-17XIAMEN UNIV OF TECH +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XIAMEN UNIV OF TECH
Filing Date
2026-06-12
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies suffer from low detection accuracy in phytoplankton imaging due to limited depth of field and random cell flipping, making it difficult to accurately identify phytoplankton and analyze population dynamics.

Method used

By setting the depth of field, numerical aperture of the objective lens, and total magnification of the microscope system, and combining the liquid flow generated by the sheath fluid injection pump, the flow rate of the liquid flow is dynamically adjusted to control the orientation of algal cells in the microscope field of view. Adaptive threshold segmentation and morphological operations are used to segment algal cells, and a lightweight large model is used for identification.

Benefits of technology

It achieves efficient, automated, and high-precision identification of phytoplankton, reduces the need for frequent focusing, and improves detection efficiency and identification accuracy.

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Abstract

本发明涉及一种用于浮游生物智能分析的光学成像方法、系统,包括以下步骤:S1,根据浮游植物的特征尺寸确定显微镜系统的景深、物镜数值孔径、总放大倍数;S2,将样品液中的浮游植物挤压形成液流,通过前置相机拍摄浮游植物的浮游植物预图像;S3,对所述浮游植物预图像进行处理,提取对应的浮游植物的生长周期特征、形态特征、活性特征;S4,根据所述生长周期特征、所述形态特征、所述活性特征,动态确定浮游植物对应的液流流速,驱动对应的浮游植物进入所述显微镜系统的视野范围,提高所述浮游植物以其最小几何尺寸沿显微镜光轴方向通过所述视野范围的概率;S5,通过所述显微镜系统采集浮游植物主图像。
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