Optical imaging of nucleic acids
By introducing nucleic acid constructs containing first and second promoters into cells, and utilizing rolling circle amplification and in situ sequencing technologies, the problem of ineffective analysis of nucleic acid expression in different cell types in existing technologies has been solved, achieving efficient nucleic acid imaging and genome screening, and improving the accuracy of cell phenotype identification.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GENENTECH INC
- Filing Date
- 2024-12-19
- Publication Date
- 2026-07-17
AI Technical Summary
Existing nucleic acid detection and characterization strategies cannot effectively analyze nucleic acid expression in different cell types, making it difficult to identify cell phenotypes associated with nucleic acid expression.
A method is provided to express a target nucleic acid under different conditions by introducing a nucleic acid construct containing first and second promoters into a cell, using these promoters, and analyzing the target nucleic acid by amplification and imaging techniques, including the use of rolling circle amplification and in situ sequencing.
It achieves efficient nucleic acid imaging and genome screening, enabling the identification and analysis of differences in nucleic acid expression and related cellular characteristics in cells, thereby improving the accuracy of nucleic acid detection and the ability to identify cell phenotypes.
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Abstract
Citation Information
Patent Citations
US4737456A