A high-pressure sterilization pot special for flower tissue culture

Abstract

The application relates to the field of flower tissue culture technology, in particular to a high-pressure sterilization pot special for flower tissue culture, which comprises a sterilization pot main body and a basket inner container arranged in the sterilization pot main body. A shelf structure is arranged in the basket inner container. The shelf structure can be used for multi-layer loading of container bottles, so that the container bottles are arranged in layers in the basket inner container. The shelf structure can be used for clamping the container bottles to limit the container bottles. The shelf structure can be used for suspending long-handle inoculation tools, so that the long-handle inoculation tools are vertically arranged. On the basis of the general high-pressure sterilization pot, the unique characteristics and specific requirements of flower tissue culture are combined to be targetedly optimized, so that the high efficiency and high quality of the flower tissue culture work are effectively supported.

Description

Technical Field

[0001] This invention relates to the field of flower tissue culture technology, and more specifically, to a high-pressure sterilizer specifically for flower tissue culture. Background Technology

[0002] Flower tissue culture is a technique that uses artificial culture media under sterile conditions to culture plant organs, tissues, or cells in vitro, causing them to divide, differentiate, or be induced into seedlings. It achieves rapid propagation through the totipotency of plant cells, offering advantages such as high efficiency and virus removal, and is widely used in the cultivation of flowers, fruits, vegetables, and traditional Chinese medicinal herbs.

[0003] Plant tissue culture technology is the core means of breeding superior flower varieties and cultivating virus-free seedlings. The construction of a sterile environment is a key prerequisite for successful tissue culture. The sterilization of culture medium, experimental equipment and culture containers directly determines the survival rate of explants and the effect of pollution control. High-pressure steam sterilization has become the most reliable physical sterilization method in flower tissue culture because it can achieve a sterilization efficiency of more than 99.9% through high temperature and high pressure.

[0004] Existing autoclaves are perfectly adequate for general laboratory sterilization needs. However, flower tissue culture requires frequent sterilization. The culture media used in flower tissue culture are stored in glass or plastic containers. The internal container of a standard autoclave is simply a liner, resulting in poor space utilization for various flower tissue culture bottles. This means only a small number of bottles can be placed at a time, and the bottles are prone to tipping over during sterilization due to steam convection, leading to spillage or container breakage. To ensure sterilization effectiveness, items must be placed vertically, not horizontally. However, the autoclave's internal liner is a porous basket; hanging long-handled forceps, scalpels, or other inoculation tools will cause them to fall in, forcing them to be placed horizontally, thus affecting sterilization efficiency. Summary of the Invention

[0005] The purpose of this invention is to provide a high-pressure sterilizer specifically for flower tissue culture to solve the above-mentioned problems.

[0006] To achieve the above objectives, the present invention provides a high-pressure sterilizer for flower tissue culture, including a sterilizer body and a basket liner disposed within the sterilizer body. The basket liner is provided with a rack structure, which can hold multiple layers of container bottles so that the container bottles are arranged in layers within the basket liner. The rack structure clamps the container bottles together to restrain them. The frame structure is capable of suspending long-handled inoculation tools, allowing the tools to be positioned vertically.

[0007] Furthermore, the frame structure includes: A long, narrow groove is vertically and circumferentially mounted on the inner wall of the basket liner; A handle, which is vertically slidably mounted on the elongated groove body; An outer frame handle, which is connected to the end of the handle; A mesh disk, the mesh disk being installed inside the outer frame handle; The extrusion clamps are hinged to the outer frame handle and close to the edge of the mesh disk, and the extrusion clamps are arranged facing each other. An outer sleeve is installed on the outer frame handle and located behind the extrusion clamp. A torsion spring is installed inside the outer sleeve. The bottom back of the extrusion clamp is connected to the free end of the torsion spring inside the outer sleeve. Applying force to the extrusion clamp can pry it back and forth. After the external force is removed, the extrusion clamp returns to its original position under the rebound of the torsion spring inside the outer sleeve. Fixed tooth blocks are fixedly installed at equal intervals on the inner bottom surface of the elongated groove, and the fixed tooth blocks are located behind the handle; A stopper, which is rotatably mounted on the handle; A lever is mounted on the stop member and is coaxial with the rotation mounting point of the stop member on the handle. By turning the lever, the stop member can be rotated to abut against the bottom surface of the handle, at which time the stop member rests on the fixed tooth block.

[0008] Furthermore, the frame structure also includes: A support rod is mounted on a rib on the back of the extrusion plate and is perpendicular to the extrusion plate. A retaining head is installed at the end of the support rod, which can fix the tail of the long-handled inoculation tool after it is inserted into the retaining head.

[0009] Furthermore, the outer frame handle and the handle are connected by a damping pivot. Horizontally bending the outer frame handle allows it to swing relative to the handle.

[0010] Furthermore, the support rod and the retaining head are connected by a ball joint.

[0011] Furthermore, a cylinder seat is installed on the ribs on the back of the extrusion plate, and a top rod is spirally installed inside the cylinder seat, with a top contact plate connected to the end of the top rod; The cylinder seat and the top rod are located below the support rod and the retaining head.

[0012] Furthermore, the clamping plate is angled and snaps inward.

[0013] Furthermore, an arc-shaped smooth fin is installed on the top of the extrusion clamp, and the arc-shaped smooth fin has the opposite orientation to the extrusion clamp.

[0014] Furthermore, the sterilizer body has rectangular draw slots facing each other, and a spring is installed on the inner bottom surface of the rectangular draw slot. A support arm is connected to the spring, and the support arm is slidably assembled in the rectangular draw slot. The top edge of the inner liner of the basket fits snugly onto the supporting lifting arm.

[0015] Compared with the prior art, the present invention has the following beneficial effects: This invention utilizes a rack-like structure to fully leverage the space within the basket, allowing for the layered arrangement of various containers containing culture media. Furthermore, the containers can be categorized and allocated according to their height. This significantly increases the number of containers that can be placed in the basket without affecting steam convection or reducing sterilization efficiency, thereby greatly increasing the number of containers that can be sterilized at once, reducing the number of sterilization operations, and effectively simplifying cumbersome procedures. Simultaneously, the rack-like structure provides external restraint for each container, ensuring its stability and effectively preventing it from being damaged by steam during sterilization. Disturbances caused by vapor convection can lead to tipping, spillage of culture medium, or breakage of containers. Furthermore, the rack structure provides a dedicated hanging function for long-handled inoculation tools such as forceps and scalpels, ensuring sterilization effectiveness and allowing for simultaneous sterilization of tools and containers, further improving the space utilization of the basket's inner liner. Rectangular slots, springs, and support arms automatically push the basket's inner liner out of the sterilizer body after sterilization and when the main body is opened, eliminating the need for manual handling and preventing burns. This also facilitates the removal of tools and containers from the basket, simplifying operation. This invention optimizes the general-purpose autoclave based on the unique characteristics and specific needs of flower tissue culture, thereby effectively supporting the efficient and high-quality conduct of flower tissue culture work. Attached Figure Description

[0016] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0017] Figure 1 A perspective view of the present invention is shown; Figure 2 A second perspective view of the present invention is shown; Figure 3 A third perspective view of the present invention is shown; Figure 4 A fourth perspective view of the present invention is shown; Figure 5 A fifth perspective view of the present invention is shown; Figure 6 A sixth perspective view of the present invention is shown; Figure 7 A seventh perspective view of the present invention is shown; Figure 8 A partial top view of the invention is shown; Figure 9 The present invention is shown. Figure 3 Enlarged view of point A; Figure 10 The present invention is shown. Figure 5 Enlarged view of point B; Figure 11 The present invention is shown. Figure 6 Enlarged view of point C.

[0018] In the figure, the same reference numerals represent the same structural element, wherein: 1. Sterilizer body; 2. Basket inner liner; 3. Frame structure; 31. Long groove; 32. Handle; 321. Damping pivot; 33. Outer frame handle; 34. Mesh plate; 35. Extrusion clamp; 36. Outer sleeve; 37. Fixed tooth block; 38. Stopper; 39. Actuating handle; 391. Support rod; 392. Fixing head; 4. Cylinder base; 5. Top rod; 6. Top connecting plate; 7. Arc-shaped smooth fins; 8. Rectangular slot; 9. Spring; 10. Support lifting arm. Detailed Implementation

[0019] The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic diagrams, illustrating only the basic structure of the invention, and therefore only show the components relevant to the invention.

[0020] like Figure 1-11 As shown, a high-pressure sterilizer for flower tissue culture includes a sterilizer body 1 and a basket inner liner 2 disposed inside the sterilizer body 1. The basket inner liner 2 is provided with a rack structure 3, which can hold multiple layers of container bottles so that the container bottles are arranged in layers inside the basket inner liner 2. The rack structure 3 clamps the container bottles together to restrain them; The rack structure 3 can suspend long-handled inoculation tools, allowing them to be placed vertically. This invention, through the rack structure 3, fully utilizes the space inside the basket liner 2, arranging various containers containing culture media in layers. Furthermore, it allows for classification and allocation based on the height of the containers. While not affecting steam convection or reducing sterilization efficiency, it significantly increases the number of containers that can be placed inside the basket liner 2, resulting in a substantial increase in the number of containers that can be sterilized at one time, reducing the number of sterilization operations, and effectively simplifying cumbersome procedures. Simultaneously, the rack structure 3 restricts the position of each container, providing a... An external limiting device ensures the stability of the container bottle, effectively preventing it from tipping over during sterilization due to steam convection disturbance, which could lead to spillage of the culture medium or breakage of the bottle. In addition, the rack structure 3 provides a hanging function specifically for long-handled inoculation tools such as forceps and scalpels, ensuring sterilization effect and allowing forceps, scalpels, and other tools and container bottles to be sterilized simultaneously, further improving the space utilization of the basket inner liner 2. Based on a general autoclave, this invention is specifically optimized to meet the unique characteristics and specific needs of flower tissue culture, thereby strongly supporting the efficient and high-quality conduct of flower tissue culture work.

[0021] Optionally, the frame structure 3 includes: The elongated groove 31 is vertically circumferentially installed on the inner wall of the basket inner liner 2; Handle 32 is vertically slidably mounted on the elongated groove 31; Outer frame handle 33, which is connected to the end of handle 32; Grid disk 34 is installed inside the outer frame handle 33; The extrusion clamp 35 is hinged to the outer frame handle 33 and close to the edge of the grid disk 34, and the extrusion clamps 35 are arranged facing each other. The outer sleeve 36 is installed on the outer frame handle 33 and located behind the extrusion clamp 35. A torsion spring is installed inside the outer sleeve 36. The bottom back of the extrusion clamp 35 is connected to the free end of the torsion spring inside the outer sleeve 36. Applying force to the extrusion clamp 35 can make it move back and forth. After the external force is removed, the extrusion clamp 35 returns to its original position under the rebound of the torsion spring inside the outer sleeve 36. Fixed tooth blocks 37 are fixedly installed at equal intervals on the inner bottom surface of the elongated groove 31, and the fixed tooth blocks 37 are located behind the handle 32. A stopper 38 is rotatably mounted on a handle 32. The actuating handle 39 is mounted on the stop member 38 and is coaxial with the rotation mounting point of the stop member 38 on the handle 32. Actuating the actuating handle 39 rotates the stop member 38 to the bottom surface of the handle 32. At this time, the stop member 38 rests on the fixed tooth block 37. Actuating the actuating handle 39 causes the stop member 38 to turn outwards. When the handle 32 moves on the elongated groove 31, the stop member 38 cannot contact the fixed tooth block 37. Grasp the handle 32 and insert it into the elongated groove 31. Actuating the actuating handle 39 rotates the stop member 38 inwards until it reaches the bottom surface of the handle 32. Then release the handle 39. 2. The stopper 38 naturally engages with the fixed tooth block 37, preventing the handle 32 from sliding further down and fixing it in its current position. Then, the two clamping plates 35 are pried open, and the prepared container bottle containing the culture medium is inserted into the two clamping plates 35 and placed on the grid plate 34. During the process of prying open the two clamping plates 35, the torsion spring inside the outer tube 36 is stretched. After releasing, the two clamping plates 35 are pulled back by the rebound action of the torsion spring, thereby clamping the container bottle in the middle with the two clamping plates 35. The elastic force restricts the position of each container bottle, ensuring the stability of the container bottle by confining it between the two clamping plates 35. This effectively prevents the containers from tipping over due to steam convection disturbance during sterilization, thus avoiding spillage of the culture medium or breakage of the containers. After placing one container, move the handle 32 to the bottom of the long trough 31 and fix it in place. Then, remove the handle 32 and place the remaining containers in sequence, fixing the next handle 32 above the previous handle 32 and the next container above the previous container, ultimately forming a layered arrangement in the inner liner 2 of the basket. When the inner liner 2 is completely full and no more can be placed inside, close the top door of the sterilizer body 1 to begin sterilization, making full use of the inner liner 2. The space is designed to allow for the layered arrangement of various containers containing culture media, significantly increasing the number of containers that can be placed in the inner liner 2 of the basket. This greatly increases the number of containers that can be sterilized at one time, reduces the number of sterilization operations, avoids the need for frequent sterilization, and effectively improves cumbersome operations. When placing containers, they can be layered according to their height, placing containers of similar height on the same layer to prevent wasted space, thereby further improving space utilization through reasonable allocation. The grid tray 34 can support the containers while not obstructing the flow of steam, so as not to affect the sterilization effect.

[0022] Optionally, the frame structure 3 also includes: Support rod 391 is installed on the rib on the back of extrusion plate 35 and is perpendicular to extrusion plate 35. The retention head 392 is installed at the end of the support rod 391. The tail of a long-handled inoculation tool is inserted into the retention head 392 and secured. After each container is placed, the tail of the long-handled inoculation tool, such as forceps or scalpels, that needs sterilization is immediately inserted into the retention head 392 and secured. The tool can then enter the inner liner 2 of the basket in a downward-facing position. The clamping plate 35, which restricts the container, provides a specific placement for long-handled inoculation tools such as forceps and scalpels. The positioning serves two purposes: it ensures that long-handled inoculation tools such as forceps and scalpels are suspended for sterilization, guaranteeing the sterilization effect, while flexibly utilizing the gaps between containers in each layer to hang small long-handled inoculation tools such as forceps and scalpels, further improving space utilization and the number of items that can be sterilized at one time; the retaining head 392 can be a magnetic connector, a self-centering clamp, a spring clamp, etc., as long as it can fix the tail of the inserted forceps, scalpel, etc. to prevent it from falling.

[0023] Optionally, the outer frame handle 33 and the handle 32 are connected by a damping pivot 321; Horizontally bending the outer frame handle 33 allows it to swing relative to the handle 32. When placing containers every other layer, after placing the containers, the outer frame handle 33 is bent to horizontally offset the outer frame handle 33 and the containers and tools on it from the containers and tools of the next layer until the containers and tools of the two layers are completely offset. This ensures that there is sufficient space above and below each layer of containers and tools, preventing them from overlapping too closely and ensuring sufficient channels for steam convection during sterilization. This significantly increases the number of items that can be sterilized at one time while preventing the sterilization effect from being affected. When the outer frame handle 33 is not bent forcefully, it will not twist arbitrarily under the restriction of the damping pivot 321.

[0024] Optionally, the support rod 391 and the retaining head 392 are connected by a universal ball joint. That is, the retaining head 392 can be flexibly and freely twisted with the support of the universal ball joint. The diameter of the container bottle is different. After the container bottle enters the clamping plate 35, the clamping plate 35 may not be in a vertical position. However, with the action of the universal ball joint, after the forceps, scalpel and other tools are loaded, the retaining head 392 can automatically adjust to a drooping position under the action of gravity, thereby improving the hanging effect of the forceps, scalpel and other tools and enhancing the sterilization effect.

[0025] Optionally, a cylinder seat 4 is installed on the ribs on the back of the extrusion plate 35, and a top rod 5 is spirally installed inside the cylinder seat 4. The end of the top rod 5 is connected to a top contact piece 6. The base 4 and the top rod 5 are located below the support rod 391 and the retaining head 392. After the forceps, scalpel and other tools are installed, the top rod 5 is rotated until the top contact plate 6 contacts the forceps, scalpel and other tools. This serves two purposes: firstly, it holds the tools in place, effectively preventing them from shaking due to steam convection during sterilization and colliding with other parts or tools; secondly, the pushing and positional restriction by the top contact plate 6 and the top rod 5, and with the support of the universal ball, further actively adjusts the vertical angle of the forceps, scalpel and other tools, so that they can be better vertical to the direction of steam, further ensuring the sterilization effect.

[0026] Optionally, the clamping plates 35 are angled and snapped together in the center. That is, in the initial state, the clamping plates 35 are also snapped together at an angle, rather than being vertically close together. Therefore, when the container bottle enters and pushes the two clamping plates 35 apart, the two clamping plates 35 use the top part of their parts to clamp the container bottle. While restricting the container bottle in the middle, it will not come into excessive contact with its surface, thus preventing the container bottle surface from being covered too much and affecting the sterilization effect of the steam.

[0027] Optionally, the top of the extrusion clamp 35 is equipped with an arc-shaped smooth fin 7. The arc-shaped smooth fin 7 is opposite in orientation to the extrusion clamp 35, so that the top of each pair of extrusion clamps 35 presents a smooth outward flaring opening. When placing the container bottle, it can be directly placed straight down along the two arc-shaped smooth fins 7 and inserted between the two extrusion clamps 35 without having to manually pry the two extrusion clamps 35 apart, thus improving convenience.

[0028] Optionally, a rectangular drawer 8 is provided facing each other inside the sterilizer body 1. A spring 9 is installed on the inner bottom surface of the rectangular drawer 8. A support lifting arm 10 is connected to the spring 9, and the support lifting arm 10 is slidably assembled in the rectangular drawer 8. The top edge of the inner basket liner 2 fits snugly onto the support lifting arm 10. When sterilization is performed with the top cover of the sterilizer body 1 closed, the two springs 9 are compressed. When the top cover of the sterilizer body 1 is opened after sterilization, the top cover opens slightly, and the two springs 9 rebound slightly, pushing the support lifting arm 10 upward, causing the inner basket liner 2 and everything inside it to move upward until the top cover is fully opened. The inner basket liner 2 is also pushed out of the sterilizer body 1. Tissue culture personnel do not need to manually remove the inner basket liner 2 from the sterilizer body 1. At this time, tissue culture personnel can take out the tools and containers inside the inner basket liner 2. The tools and containers can be placed directly into the inner basket liner 2 for the next sterilization. This avoids a lot of manual handling of the inner basket liner 2, preventing burns and making it more convenient and faster.

[0029] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A high-pressure sterilizer for flower tissue culture, comprising a sterilizer body (1) and a basket inner liner (2) disposed within the sterilizer body (1), characterized in that: The inner liner (2) of the basket is provided with a rack structure (3), which can hold multiple layers of container bottles so that the container bottles are arranged in layers inside the inner liner (2). The frame structure (3) clamps the container bottles together to restrict their movement; The frame structure (3) is capable of suspending the long-handled inoculation tool so that the long-handled inoculation tool is placed vertically.

2. The high-pressure sterilizer for flower tissue culture as described in claim 1, characterized in that, The frame structure (3) includes: A long groove (31) is vertically circumferentially installed on the inner wall of the basket liner (2); Handle (32), which is vertically slidably mounted on the elongated groove (31); The outer frame handle (33) is connected to the end of the handle (32); A mesh disk (34) is installed inside the outer frame handle (33); Extrusion clamp (35), the extrusion clamp (35) is hinged to the outer frame handle (33) and close to the edge of the grid disk (34), and the extrusion clamp (35) is arranged facing each other; The outer tube (36) is installed on the outer frame handle (33) and located behind the extrusion plate (35). A torsion spring is installed inside the outer tube (36). The bottom back of the extrusion plate (35) is connected to the free end of the torsion spring inside the outer tube (36). Applying force to the extrusion plate (35) can make it move back and forth. After the external force is removed, the extrusion plate (35) returns to its original position under the rebound of the torsion spring inside the outer tube (36). Fixed tooth blocks (37) are fixedly installed at equal intervals on the inner bottom surface of the elongated groove (31), and the fixed tooth blocks (37) are located behind the handle (32); A stopper (38) is rotatably mounted on the handle (32); A lever (39) is mounted on the stop (38) and is coaxial with the rotation mounting point of the stop (38) on the handle (32). By turning the lever (39), the stop (38) can be rotated to abut against the bottom surface of the handle (32), at which time the stop (38) rests on the fixed tooth block (37).

3. The high-pressure sterilizer for flower tissue culture as described in claim 2, characterized in that, The frame structure (3) also includes: Support rod (391), which is mounted on the rib on the back of the extrusion plate (35) and is perpendicular to the extrusion plate (35). The retention head (392) is installed at the end of the support rod (391) and can be fixed after the tail of the long-handled inoculation tool is inserted into the retention head (392).

4. The high-pressure sterilizer for flower tissue culture as described in claim 3, characterized in that, The outer frame handle (33) and the handle (32) are connected by a damping pivot (321); Horizontally bending the outer frame handle (33) allows it to swing relative to the handle (32).

5. The high-pressure sterilizer for flower tissue culture as described in claim 4, characterized in that, The support rod (391) and the retaining head (392) are connected by a ball joint.

6. The high-pressure sterilizer for flower tissue culture as described in claim 5, characterized in that, A cylinder seat (4) is installed on the rib on the back of the extrusion plate (35), and a top rod (5) is spirally installed inside the cylinder seat (4). A top contact piece (6) is connected to the end of the top rod (5). The cylinder seat (4) and the top rod (5) are located below the support rod (391) and the retaining head (392).

7. The high-pressure sterilizer for flower tissue culture as described in claim 6, characterized in that, The extrusion clamp (35) is obliquely arranged and snapped inward.

8. The autoclave for flower tissue culture as described in claim 7, characterized in that, The top of the extrusion plate (35) is equipped with an arc-shaped smooth fin (7), which is opposite in orientation to the extrusion plate (35).

9. The high-pressure sterilizer for flower tissue culture as described in claim 8, characterized in that, The sterilizer body (1) has a rectangular drawer (8) facing each other inside. A spring (9) is installed on the inner bottom surface of the rectangular drawer (8). A support arm (10) is connected to the spring (9), and the support arm (10) is slidably assembled in the rectangular drawer (8). The top edge of the inner liner of the basket (2) is fitted and secured to the support arm (10).

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