A multi-component composition for brain health and intelligence and a method of preparing the same

Abstract

The application discloses a kind of brain health and intelligence's multiple composition and preparation method thereof.The application uses Schisandra chinensis, Gastrodia elata, Jujube Seed as Chinese medicinal material, flaxseed, salmon protein as protein source, with lecithin, phosphatidylserine and other functional ingredients, by active ingredient is encapsulated in phospholipid nanoparticle, again with the transferrin provided by pig blood extract and milk fat globule membrane target ligand and assembly constructs targeted delivery system, then add Armillaria mellea polysaccharide, milk fat globule membrane extraction by-product to obtain finished product.The application realizes immune escape using the natural source characteristics of milk fat globule membrane, realizes the active targeting of blood-brain barrier using the related protein of pig blood extract, and the obtained product can be directly freeze-dried as an intermediate, or added to various functional foods, and can realize brain health and intelligence, improve brain fatigue, tranquilizing and sleep aiding and other effects by multi-target point synergy, suitable for aging population, teenagers and job fatigue population.

Description

Technical Field

[0001] This invention belongs to the field of food processing technology, specifically relating to a multi-component composition for improving brain function and intelligence, and its preparation method. Background Technology

[0002] With the advent of the information age, the intensity of mental activity has increased significantly, coupled with the accelerating aging process, leading to increasingly prominent issues related to brain function decline. Long-term mental exhaustion, irregular sleep patterns, and poor blood circulation can easily result in insufficient oxygen supply to the brain, causing symptoms such as memory loss, difficulty concentrating, slow reaction time, and mental fatigue. In severe cases, it may even induce potential risks such as cognitive impairment and Alzheimer's disease. Meanwhile, different groups, including teenagers under heavy academic pressure, middle-aged and elderly people experiencing natural brain function decline, and office workers facing long-term high-pressure work, all face an urgent need for brain care.

[0003] Currently, existing brain-boosting and intelligence-enhancing products generally suffer from poor stability of core ingredients, rapid metabolism in the body, and difficulty in crossing the blood-brain barrier. They are often degraded by stomach acid or metabolized by the first-pass effect of the liver in the digestive tract before reaching their target, ultimately becoming "expensive waste." The current industry bottleneck lies in low bioavailability; active ingredients struggle to penetrate the intestinal mucosa and enter the bloodstream, let alone efficiently cross the blood-brain barrier to exert their effects.

[0004] The market for brain-boosting products has significant limitations: some products rely on single nutrients (such as DHA or lecithin alone), offering limited efficacy and lacking multidimensional coverage of "intelligence enhancement, oxygen supply, and anti-fatigue"; some formulations lack scientific compatibility, failing to fully utilize the synergistic effects of medicinal and edible ingredients and modern active substances; and some products add chemically-based nootropic ingredients, raising safety concerns and making them unsuitable for long-term use. Furthermore, the combination of medicinal and edible ingredients with natural active peptides and functional lipids offers unique advantages in brain health, regulating organ function through traditional Chinese medicine components while directly nourishing nerves and improving circulation through modern active substances. However, existing products have not fully achieved the synergistic effect of "nerve nourishment, improved circulation, and anti-aging and intelligence enhancement," lacking targeted design for brain oxygen supply mechanisms and nerve cell metabolism. Therefore, developing a compound composition based on the traditional Chinese medicine theory of "brain health and opening of the orifices" and modern nutritional principles, with scientific compatibility, high safety, and dual effects of enhancing brain function and increasing brain oxygen supply, suitable for long-term use, has become an urgent market need. Summary of the Invention

[0005] The technical problem to be solved: Addressing the shortcomings of existing brain-boosting and intelligence-enhancing products, such as low bioavailability, difficulty in penetrating the blood-brain barrier, single-component formulations, and unstable molding systems, this invention uses Alpinia oxyphylla, Gastrodia elata, and Ziziphus jujuba var. spinosa as core traditional Chinese medicine ingredients, flaxseed and salmon protein as protein sources, and functional components such as lecithin and phosphatidylserine. Through targeted enzymatic hydrolysis, flash extraction, complex encapsulation, and modification with pig blood extract, a highly efficient brain-targeted delivery system is constructed. Furthermore, Armillaria mellea polysaccharide and milk fat globule membrane extract byproducts are used as core components, synergistically combined with kudzu root powder to create a completely natural and stable product form. The final product exhibits excellent stability and can synergistically achieve multiple target-specific effects such as brain enhancement, improvement of brain fatigue, and calming and sleep aid.

[0006] Technical solution: A method for preparing a multi-component composition for brain health and intelligence enhancement, comprising the following steps: S1. Peptide preparation: Flaxseed powder and salmon protein were mixed, water was added and stirred evenly, a complex protease was added, and the mixture was stirred at a constant temperature for enzymatic hydrolysis. After completion, the enzyme was inactivated by heating, cooled, centrifuged, and the supernatant was collected and filtered twice through an ultrafiltration membrane. The peptide solution was collected and freeze-dried to obtain flaxseed-salmon enzymatic hydrolysed peptide powder. S2. Preparation of Chinese herbal extract: Alpinia oxyphylla, Gastrodia elata, and Ziziphus jujuba seeds were pulverized and sieved to obtain mixed powder. Water was added and flash extraction was performed. After the extraction was completed, the supernatant A was collected by centrifugation. Ethanol solution was added to the extracted residue and flash extraction was performed again. After the extraction was completed, the supernatant B was collected by centrifugation. Supernatant A and supernatant B were mixed and rotary evaporated to obtain Chinese herbal extract. S3. Extraction of milk fat globule membrane: The light cream separated from raw milk is repeatedly washed and centrifuged with phosphate buffer to obtain milk fat globules. The washed phosphate buffer is collected and refrigerated for later use. The milk fat globules are ultrasonically broken up and centrifuged. The lower layer mixture is collected, concentrated, and freeze-dried to obtain milk fat globule membrane powder. S4. Preparation of colostrum: The extract of traditional Chinese medicine and epigallocatechin gallate were added to water and stirred evenly. Flaxseed-salmon enzymatic hydrolysate powder was added and homogenized to obtain an aqueous solution. Lecithin, phosphatidylserine and N-acetylneuraminic acid were added to anhydrous ethanol and stirred in a water bath at a constant temperature to obtain a composite phospholipid solution. The aqueous solution was slowly added dropwise to the composite phospholipid solution and high-speed shearing was used to form a colostrum. S5. Preparation of delivery system: The primary emulsion and milk fat globule membrane solution were mixed, ultrasonically cavitated, and stirred at a constant temperature to obtain an emulsion. The pig blood extract was dissolved in phosphate buffer to obtain a modification solution. The emulsion and modification solution were mixed, stirred and incubated, homogenized, and then filtered through a microporous membrane to obtain the delivery system. S6. Preparation of multi-component composition: The polysaccharide of Armillaria mellea is mixed evenly with the washed phosphate buffer solution and stirred in a water bath to obtain a mixture. The kudzu root powder is heated with water to gelatinize, and stirred continuously during the process. The gelatinized kudzu root powder is mixed with the mixture and stirred continuously. The mixture is then slowly added to the delivery system, emulsified and sheared to obtain the brain-boosting and intelligence-enhancing multi-component composition.

[0007] Further, in step S1, the mass ratio of flaxseed powder, salmon protein, and water is (5-8):(2-4):(70-100); the complex protease includes neutral protease, alkaline protease, and trypsin, and the amount of complex protease added is 3-5 wt.%; the constant temperature stirring temperature is 35-45℃, the stirring speed is 200-300 rpm, and the time is 3-4 h; the heating temperature is 75-85℃, and the enzyme inactivation time is 15-25 min; the centrifugation speed is 7000-8000 rpm, and the time is 10-15 min; the molecular weight of the polypeptide solution is 1000-3000 Da.

[0008] Furthermore, the mass ratio of the neutral protease, alkaline protease, and trypsin is (2-3):(1-2):(1-2).

[0009] Furthermore, in step S2, the mass ratio of Alpinia oxyphylla, Gastrodia elata, and Ziziphus jujuba var. spinosa is (6-16):(5-15):(7-18), the mass ratio of the mixed medicinal powder to water is 1:(6-8), the mass ratio of the residue to the ethanol solution is 1:(5-7), the concentration of the ethanol solution is 70-85%, and the flash extraction speed is 3000-5000 rpm for 5-10 min.

[0010] Furthermore, in step S3, the pH of the phosphate buffer solution is 6.5-7.5; washing is performed 2-3 times; the centrifugation speed is 8000-9000 rpm for 10-15 min; the ultrasonic disruption power is 300-500W, the ultrasonic working time is 5 s, the interval time is 3 s, and the total ultrasonic time is 10-15 min.

[0011] Furthermore, in step S4, the mass ratio of the herbal extract, epigallocatechin gallate, flaxseed-salmon enzymatic hydrolysate powder, and water is (3-4):(0.2-0.5):(2-3):(80-100); the mass ratio of lecithin, phosphatidylserine, N-acetylneuraminic acid, and anhydrous ethanol is (8-10):(3-5):(2-3):(100-120); the mass ratio of the composite phospholipid solution to the aqueous phase is (1-3):1; the homogenization pressure is 5-10 MPa; the high-speed shearing speed is 8000-9000 rpm, and the time is 5-10 min.

[0012] Furthermore, in step S5, the concentration of the milk fat globule membrane solution is 1-2 wt.%; the pH of the phosphate buffer is 7-7.5; the concentration of the porcine blood extract in the modification solution is 2-5 wt.%; the mass ratio of the primary emulsion to the milk fat globule membrane solution is (1-2):(1-2); the mass ratio of the emulsion to the modification solution is (3-5):1; the ultrasonic power is 200-300W, and the ultrasonic time is 10-15 min; the constant temperature stirring temperature is 30-35℃, and the time is 30-50 min; the stirring speed is 100-200 rpm; the incubation time is 2-3 h; and the homogenization pressure is 20-25 MPa.

[0013] Furthermore, in step S6, the mass ratio of Armillaria mellea polysaccharide to washed phosphate buffer is (1-2):(8-9); the water bath stirring temperature is 40-50℃, the stirring speed is 300-500rpm, and the time is 20-30min; the mass ratio of kudzu root powder to water is 1:(8-10); the mass ratio of gelatinized kudzu root powder, mixture, and delivery system is 1:(5-8):(10-15); the emulsification shearing speed is 9000-10000rpm, and the time is 15-20min.

[0014] The above-described preparation method yields a multi-component composition for improving brain function and intelligence.

[0015] The brain-boosting and intelligence-enhancing multi-component composition described above can be further processed into oral liquids, granules, tablets, or used as ingredients in functional foods.

[0016] Beneficial effects: 1. This invention uses milk fat globule membrane, which is a three-layer membrane structure composed of phospholipids, sphingomyelin, cholesterol and specific proteins. It is very similar to nerve cell membranes and is rich in sphingomyelin, a component that is extremely abundant in the brain. This helps to mimic the brain environment, thereby reducing immune rejection and promoting intracellular uptake. Its specific components (such as sphingomyelin) may help maintain the health of brain vascular endothelial cells and the integrity of tight connections.

[0017] 2. This invention forms a complete closed-loop process from raw material enzymatic hydrolysis, traditional Chinese medicine extraction, active encapsulation and targeted delivery to a stable finished product. Each step of the process is designed around the core objectives of "easy absorption, brain penetration, stable release, and enhanced safety," fundamentally solving the technical pain points of traditional brain-boosting products such as poor absorption, difficulty in penetrating the blood-brain barrier, slow onset of action, and unstable system. Moreover, all raw materials are either medicinal and edible or food-grade functional ingredients, and the core raw materials are all of natural origin, ensuring high safety and long-term consumption.

[0018] 3. This invention achieves multi-target synergistic brain health, with comprehensive and strong synergistic effects. It can simultaneously achieve multiple functions such as small molecule energy supply, BDNF upregulation, nerve membrane repair, improved brain circulation, anti-oxidation, and calming and sleep aiding: enzymatically hydrolyzed flaxseed-salmon small molecule peptides can quickly supply energy to the brain, improve brain fatigue, and enhance attention; puerarin upregulates BDNF through the calcium signaling pathway, promoting nerve repair and memory consolidation; phospholipids and phosphatidylserine can repair nerve cell membranes and promote nerve signal transmission; extracts of traditional Chinese medicines such as Gastrodia elata, Ziziphus jujuba var. spinosa, and Alpinia oxyphylla can improve brain circulation, calm the mind and aid sleep, and relieve anxiety; EGCG can have antioxidant, anti-inflammatory, and nerve cell protective effects; combined with the sustained-release properties of the gel and the long-lasting circulation properties of the targeted delivery system, it achieves long-lasting brain protection with a single dose.

[0019] 4. The final product system prepared by this invention has excellent stability. Kudzu root powder and Armillaria mellea polysaccharide can effectively encapsulate various active ingredients, forming a physical protective barrier, reducing the loss of active ingredients during storage and digestion, and significantly improving the shelf life and utilization rate of active ingredients. At the same time, Armillaria mellea polysaccharide and kudzu root powder effectively mask the bitter taste of traditional Chinese medicine ingredients, avoiding the problem of rough taste and unacceptable flavor of traditional brain health products, and significantly improving user experience and compliance.

[0020] 5. This invention employs a targeted enzymatic hydrolysis process using a combination of neutral protease, alkaline protease, and trypsin to directionally cleave flaxseed and salmon proteins, avoiding excessive degradation and the generation of ineffective peptides. The molecular weight of the hydrolysate is precisely controlled within the optimal range of 1000-3000 Da, within which peptides can be directly absorbed by the intestines and efficiently penetrate the blood-brain barrier. Furthermore, the synergistic enzymatic hydrolysis of flaxseed and salmon proteins achieves amino acid complementarity, resulting in a more comprehensive supply of brain nutrition. The small molecule peptides can quickly replenish energy to the brain, rapidly improve brain fatigue, and enhance attention and memory.

[0021] 6. This invention uses phospholipid-milk fat globule membrane as a composite encapsulation substrate and porcine blood extract as a targeted modified receptor protein, resulting in a delivery system with extremely high biocompatibility. Compared to traditional cell membrane encapsulation methods for drug delivery, milk fat globule membrane combines natural biocompatibility, emulsification stability, and nutritional functionality. The encapsulation process is simpler, the raw materials are easier to obtain, and the stability of the encapsulated system is superior, effectively improving the water solubility and storage stability of hydrophobic active ingredients. Targeted modification enhances the system's receptor-mediated transcytosis capacity at the blood-brain barrier, significantly improving the intestinal absorption efficiency and blood-brain barrier penetration rate of active ingredients. The dense membrane layer of the composite encapsulation system effectively protects lipid-soluble and easily degradable active ingredients from destruction by gastric acid and digestive enzymes, achieving long-term circulation and slow release of active ingredients, making the brain-boosting effects more lasting. Detailed Implementation

[0022] The present invention will be further described below with reference to embodiments. These embodiments are illustrative of the present invention, but the present invention is not limited to these embodiments: Example 1

[0023] A multi-component composition for brain health and intelligence enhancement, and its preparation method: S1. Peptide preparation: 5 parts flaxseed powder and 2 parts salmon protein were mixed, 70 parts water were added and stirred evenly, 1.5 parts neutral protease, 0.5 parts alkaline protease and 0.5 parts trypsin were added, and the mixture was kept at 35℃ and stirred at 200 rpm for 3 hours for enzymatic hydrolysis. After completion, the temperature was raised to 75℃ to inactivate the enzyme for 15 minutes, cooled, and centrifuged at 8000 rpm for 15 minutes to collect the supernatant. The supernatant was then double filtered through an ultrafiltration membrane to retain peptides of 1000-3000 Da. The peptide solution was collected and freeze-dried to obtain flaxseed-salmon enzymatic hydrolysed peptide powder. S2. Preparation of Chinese herbal extract: 9 parts of Alpinia oxyphylla, 6 parts of Gastrodia elata, and 7 parts of Ziziphus jujuba var. spinosa were pulverized and passed through a 60-mesh sieve to obtain a mixed powder. 135 parts of water were added and flash extraction was performed at a speed of 4000 rpm for 10 min. After the extraction was completed, the supernatant A was collected by centrifugation. Five times the amount of ethanol solution was added to the extracted residue, and flash extraction was performed again. After the extraction was completed, the supernatant B was collected by centrifugation. Supernatant A and supernatant B were mixed and rotary evaporated to obtain the Chinese herbal extract. S3. Extraction of milk fat globule membrane: The light cream separated from raw milk was washed three times with phosphate buffer at pH 6.5, centrifuged at 8000 rpm for 10 min to obtain milk fat globules, and the washed phosphate buffer was collected and refrigerated for later use. The milk fat globules were sonicated at 500W for 15 min with a working time of 5 s and an interval of 3 s. The lower layer mixture was collected by centrifugation, concentrated, and freeze-dried to obtain milk fat globule membrane powder. S4. Preparation of colostrum: 4 parts of Chinese herbal extract and 0.5 parts of epigallocatechin gallate were added to 80 parts of water and stirred evenly. 2 parts of flaxseed-salmon enzymatic hydrolysate powder were added and homogenized at 8 MPa to obtain an aqueous solution. 4 parts of lecithin, 2 parts of phosphatidylserine, and 1 part of N-acetylneuraminic acid were added to 50 parts of anhydrous ethanol and stirred at a constant temperature of 35℃ in a water bath to obtain a composite phospholipid solution. 3 parts of the aqueous solution were slowly added dropwise to 6 parts of the composite phospholipid solution and sheared at 8000 rpm for 10 min to form the colostrum. S5. Preparation of delivery system: 8 parts of primary emulsion and 8 parts of 2 wt.% milk fat globule membrane solution were mixed, ultrasonically cavitated at 300W for 10 min, and then stirred at 35℃ for 30 min to obtain emulsion. Pig blood extract was dissolved in phosphate buffer to obtain a 3 wt.% modification solution. 15 parts of emulsion and 3 parts of modification solution were mixed, stirred and incubated at 100 rpm for 3 h, homogenized at 20 MPa, and then filtered through a microporous membrane to obtain the delivery system. S6. Preparation of multi-component composition: 2 parts of Armillaria mellea polysaccharide and 8 parts of washed phosphate buffer were mixed evenly and stirred in a 40°C water bath at 500 rpm for 20 min to obtain a mixture. 1 part of kudzu root powder was heated with 9 parts of water to gelatinize, and stirring was continued during the process. 1 part of gelatinized kudzu root powder was mixed with 5 parts of the mixture and stirred continuously. 10 parts of the delivery system were slowly added and emulsified and sheared at 10,000 rpm for 15 min to obtain the brain-boosting and intelligence-enhancing multi-component composition. Example 2

[0024] A multi-component composition for brain health and intelligence enhancement, and its preparation method: S1. Peptide preparation: 8 parts flaxseed powder and 2 parts salmon protein were mixed, 70 parts water were added and stirred evenly, 1.5 parts neutral protease, 0.5 parts alkaline protease and 0.5 parts trypsin were added, and the mixture was kept at 35℃ and stirred at 200 rpm for 3 hours for enzymatic hydrolysis. After completion, the temperature was raised to 75℃ to inactivate the enzyme for 15 minutes, cooled, and centrifuged at 8000 rpm for 15 minutes to collect the supernatant. The supernatant was then double filtered through an ultrafiltration membrane to retain peptides of 1000-3000 Da. The peptide solution was collected and freeze-dried to obtain flaxseed-salmon enzymatic hydrolysed peptide powder. S2. Preparation of Chinese herbal extract: 9 parts of Alpinia oxyphylla, 6 parts of Gastrodia elata, and 7 parts of Ziziphus jujuba var. spinosa were pulverized and passed through a 60-mesh sieve to obtain a mixed powder. 135 parts of water were added and flash extraction was performed at a speed of 4000 rpm for 10 min. After the extraction was completed, the supernatant A was collected by centrifugation. Five times the amount of ethanol solution was added to the extracted residue, and flash extraction was performed again. After the extraction was completed, the supernatant B was collected by centrifugation. Supernatant A and supernatant B were mixed and rotary evaporated to obtain the Chinese herbal extract. S3. Extraction of milk fat globule membrane: The light cream separated from raw milk was washed three times with phosphate buffer at pH 6.5, centrifuged at 8000 rpm for 10 min to obtain milk fat globules, and the washed phosphate buffer was collected and refrigerated for later use. The milk fat globules were sonicated at 500W for 15 min with a working time of 5 s and an interval of 3 s. The lower layer mixture was collected by centrifugation, concentrated, and freeze-dried to obtain milk fat globule membrane powder. S4. Preparation of colostrum: 4 parts of Chinese herbal extract and 0.5 parts of epigallocatechin gallate were added to 80 parts of water and stirred evenly. 2 parts of flaxseed-salmon enzymatic hydrolysate powder were added and homogenized at 8 MPa to obtain an aqueous solution. 4 parts of lecithin, 2 parts of phosphatidylserine, and 1 part of N-acetylneuraminic acid were added to 50 parts of anhydrous ethanol and stirred at a constant temperature of 35℃ in a water bath to obtain a composite phospholipid solution. 3 parts of the aqueous solution were slowly added dropwise to 6 parts of the composite phospholipid solution and sheared at 8000 rpm for 10 min to form the colostrum. S5. Preparation of delivery system: 8 parts of primary emulsion and 8 parts of 2 wt.% milk fat globule membrane solution were mixed, ultrasonically cavitated at 300W for 10 min, and then stirred at 35℃ for 30 min to obtain emulsion. Pig blood extract was dissolved in phosphate buffer to obtain a 3 wt.% modification solution. 15 parts of emulsion and 3 parts of modification solution were mixed, stirred and incubated at 100 rpm for 3 h, homogenized at 20 MPa, and then filtered through a microporous membrane to obtain the delivery system. S6. Preparation of multi-component composition: 2 parts of Armillaria mellea polysaccharide and 8 parts of washed phosphate buffer were mixed evenly and stirred in a 40°C water bath at 500 rpm for 20 min to obtain a mixture. 1 part of kudzu root powder was heated with 9 parts of water to gelatinize, and stirring was continued during the process. 1 part of gelatinized kudzu root powder was mixed with 5 parts of the mixture and stirred continuously. 10 parts of the delivery system were slowly added and emulsified and sheared at 10,000 rpm for 15 min to obtain the brain-boosting and intelligence-enhancing multi-component composition. Example 3

[0025] A multi-component composition for brain health and intelligence enhancement, and its preparation method: S1. Peptide preparation: 5 parts flaxseed powder and 2 parts salmon protein were mixed, 70 parts water were added and stirred evenly, 1.5 parts neutral protease, 0.5 parts alkaline protease and 0.5 parts trypsin were added, and the mixture was kept at 35℃ and stirred at 200 rpm for 3 hours for enzymatic hydrolysis. After completion, the temperature was raised to 75℃ to inactivate the enzyme for 15 minutes, cooled, and centrifuged at 8000 rpm for 15 minutes to collect the supernatant. The supernatant was then double filtered through an ultrafiltration membrane to retain peptides of 1000-3000 Da. The peptide solution was collected and freeze-dried to obtain flaxseed-salmon enzymatic hydrolysed peptide powder. S2. Preparation of Chinese herbal extract: 9 parts of Alpinia oxyphylla, 6 parts of Gastrodia elata, and 7 parts of Ziziphus jujuba var. spinosa were pulverized and passed through a 60-mesh sieve to obtain a mixed powder. 135 parts of water were added and flash extraction was performed at a speed of 4000 rpm for 10 min. After the extraction was completed, the supernatant A was collected by centrifugation. Five times the amount of ethanol solution was added to the extracted residue, and flash extraction was performed again. After the extraction was completed, the supernatant B was collected by centrifugation. Supernatant A and supernatant B were mixed and rotary evaporated to obtain the Chinese herbal extract. S3. Extraction of milk fat globule membrane: The light cream separated from raw milk was washed three times with phosphate buffer at pH 6.5, centrifuged at 8000 rpm for 10 min to obtain milk fat globules, and the washed phosphate buffer was collected and refrigerated for later use. The milk fat globules were sonicated at 500W for 15 min with a working time of 5 s and an interval of 3 s. The lower layer mixture was collected by centrifugation, concentrated, and freeze-dried to obtain milk fat globule membrane powder. S4. Preparation of colostrum: 4 parts of Chinese herbal extract and 0.5 parts of epigallocatechin gallate were added to 80 parts of water and stirred evenly. 3 parts of flaxseed-salmon enzymatic hydrolysate powder were added and homogenized at 8 MPa to obtain an aqueous solution. 4 parts of lecithin, 2 parts of phosphatidylserine, and 1 part of N-acetylneuraminic acid were added to 50 parts of anhydrous ethanol and stirred at a constant temperature of 35℃ in a water bath to obtain a composite phospholipid solution. 3 parts of the aqueous solution were slowly added dropwise to 6 parts of the composite phospholipid solution and sheared at 8000 rpm for 10 min to form the colostrum. S5. Preparation of delivery system: 8 parts of primary emulsion and 8 parts of 2 wt.% milk fat globule membrane solution were mixed, ultrasonically cavitated at 300W for 10 min, and then stirred at 35℃ for 30 min to obtain emulsion. Pig blood extract was dissolved in phosphate buffer to obtain a 3 wt.% modification solution. 15 parts of emulsion and 3 parts of modification solution were mixed, stirred and incubated at 100 rpm for 3 h, homogenized at 20 MPa, and then filtered through a microporous membrane to obtain the delivery system. S6. Preparation of multi-component composition: 2 parts of Armillaria mellea polysaccharide and 8 parts of washed phosphate buffer were mixed evenly and stirred in a 40°C water bath at 500 rpm for 20 min to obtain a mixture. 1 part of kudzu root powder was heated with 9 parts of water to gelatinize, and stirring was continued during the process. 1 part of gelatinized kudzu root powder was mixed with 5 parts of the mixture and stirred continuously. 10 parts of the delivery system were slowly added and emulsified and sheared at 10,000 rpm for 15 min to obtain the brain-boosting and intelligence-enhancing multi-component composition. Example 4

[0026] A multi-component composition for brain health and intelligence enhancement, and its preparation method: S1. Peptide preparation: 5 parts flaxseed powder and 2 parts salmon protein were mixed, 70 parts water were added and stirred evenly, 1.5 parts neutral protease, 0.5 parts alkaline protease and 0.5 parts trypsin were added, and the mixture was kept at 35℃ and stirred at 200 rpm for 3 hours for enzymatic hydrolysis. After completion, the temperature was raised to 75℃ to inactivate the enzyme for 15 minutes, cooled, and centrifuged at 8000 rpm for 15 minutes to collect the supernatant. The supernatant was then double filtered through an ultrafiltration membrane to retain peptides of 1000-3000 Da. The peptide solution was collected and freeze-dried to obtain flaxseed-salmon enzymatic hydrolysed peptide powder. S2. Preparation of Chinese herbal extract: 9 parts of Alpinia oxyphylla, 6 parts of Gastrodia elata, and 7 parts of Ziziphus jujuba var. spinosa were pulverized and passed through a 60-mesh sieve to obtain a mixed powder. 135 parts of water were added and flash extraction was performed at a speed of 4000 rpm for 10 min. After the extraction was completed, the supernatant A was collected by centrifugation. Five times the amount of ethanol solution was added to the extracted residue, and flash extraction was performed again. After the extraction was completed, the supernatant B was collected by centrifugation. Supernatant A and supernatant B were mixed and rotary evaporated to obtain the Chinese herbal extract. S3. Extraction of milk fat globule membrane: The light cream separated from raw milk was washed three times with phosphate buffer at pH 6.5, centrifuged at 8000 rpm for 10 min to obtain milk fat globules, and the washed phosphate buffer was collected and refrigerated for later use. The milk fat globules were sonicated at 500W for 15 min with a working time of 5 s and an interval of 3 s. The lower layer mixture was collected by centrifugation, concentrated, and freeze-dried to obtain milk fat globule membrane powder. S4. Preparation of colostrum: 4 parts of Chinese herbal extract and 0.5 parts of epigallocatechin gallate were added to 80 parts of water and stirred evenly. 2 parts of flaxseed-salmon enzymatic hydrolysate powder were added and homogenized at 8 MPa to obtain an aqueous solution. 4 parts of lecithin, 2 parts of phosphatidylserine, and 1 part of N-acetylneuraminic acid were added to 50 parts of anhydrous ethanol and stirred at a constant temperature of 35℃ in a water bath to obtain a composite phospholipid solution. 3 parts of the aqueous solution were slowly added dropwise to 6 parts of the composite phospholipid solution and sheared at 8000 rpm for 10 min to form the colostrum. S5. Preparation of delivery system: 8 parts of primary emulsion and 8 parts of 2 wt.% milk fat globule membrane solution were mixed, ultrasonically cavitated at 300W for 10 min, and then stirred at 35℃ for 30 min to obtain emulsion. Pig blood extract was dissolved in phosphate buffer to obtain a 3 wt.% modification solution. 9 parts of emulsion and 3 parts of modification solution were mixed, stirred and incubated at 100 rpm for 3 h, homogenized at 20 MPa, and then filtered through a microporous membrane to obtain the delivery system. S6. Preparation of multi-component composition: 2 parts of Armillaria mellea polysaccharide and 8 parts of washed phosphate buffer were mixed evenly and stirred in a 40°C water bath at 500 rpm for 20 min to obtain a mixture. 1 part of kudzu root powder was heated with 9 parts of water to gelatinize, and stirring was continued during the process. 1 part of gelatinized kudzu root powder was mixed with 5 parts of the mixture and stirred continuously. 10 parts of the delivery system were slowly added and emulsified and sheared at 10,000 rpm for 15 min to obtain the brain-boosting and intelligence-enhancing multi-component composition. Example 5

[0027] A multi-component composition for brain health and intelligence enhancement, and its preparation method: S1. Peptide preparation: 5 parts flaxseed powder and 2 parts salmon protein were mixed, 70 parts water were added and stirred evenly, 1.5 parts neutral protease, 0.5 parts alkaline protease and 0.5 parts trypsin were added, and the mixture was kept at 35℃ and stirred at 200 rpm for 3 hours for enzymatic hydrolysis. After completion, the temperature was raised to 75℃ to inactivate the enzyme for 15 minutes, cooled, and centrifuged at 8000 rpm for 15 minutes to collect the supernatant. The supernatant was then double filtered through an ultrafiltration membrane to retain peptides of 1000-3000 Da. The peptide solution was collected and freeze-dried to obtain flaxseed-salmon enzymatic hydrolysed peptide powder. S2. Preparation of Chinese herbal extract: 9 parts of Alpinia oxyphylla, 6 parts of Gastrodia elata, and 7 parts of Ziziphus jujuba var. spinosa were pulverized and passed through a 60-mesh sieve to obtain a mixed powder. 135 parts of water were added and flash extraction was performed at a speed of 4000 rpm for 10 min. After the extraction was completed, the supernatant A was collected by centrifugation. Five times the amount of ethanol solution was added to the extracted residue, and flash extraction was performed again. After the extraction was completed, the supernatant B was collected by centrifugation. Supernatant A and supernatant B were mixed and rotary evaporated to obtain the Chinese herbal extract. S3. Extraction of milk fat globule membrane: The light cream separated from raw milk was washed three times with phosphate buffer at pH 6.5, centrifuged at 8000 rpm for 10 min to obtain milk fat globules, and the washed phosphate buffer was collected and refrigerated for later use. The milk fat globules were sonicated at 500W for 15 min with a working time of 5 s and an interval of 3 s. The lower layer mixture was collected by centrifugation, concentrated, and freeze-dried to obtain milk fat globule membrane powder. S4. Preparation of colostrum: 4 parts of Chinese herbal extract and 0.5 parts of epigallocatechin gallate were added to 80 parts of water and stirred evenly. 2 parts of flaxseed-salmon enzymatic hydrolysate powder were added and homogenized at 8 MPa to obtain an aqueous solution. 4 parts of lecithin, 2 parts of phosphatidylserine, and 1 part of N-acetylneuraminic acid were added to 50 parts of anhydrous ethanol and stirred at a constant temperature of 35℃ in a water bath to obtain a composite phospholipid solution. 3 parts of the aqueous solution were slowly added dropwise to 6 parts of the composite phospholipid solution and sheared at 8000 rpm for 10 min to form the colostrum. S5. Preparation of delivery system: 8 parts of primary emulsion and 8 parts of 2 wt.% milk fat globule membrane solution were mixed, ultrasonically cavitated at 300W for 10 min, and then stirred at 35℃ for 30 min to obtain emulsion. Pig blood extract was dissolved in phosphate buffer to obtain a 3 wt.% modification solution. 15 parts of emulsion and 3 parts of modification solution were mixed, stirred and incubated at 100 rpm for 3 h, homogenized at 20 MPa, and then filtered through a microporous membrane to obtain the delivery system. S6. Preparation of multi-component composition: 2 parts of Armillaria mellea polysaccharide and 8 parts of washed phosphate buffer were mixed evenly and stirred at 500 rpm for 20 min in a 40°C water bath to obtain a mixture. 1 part of kudzu root powder was heated with 9 parts of water to gelatinize, and stirring was continued during the process. 1 part of gelatinized kudzu root powder was mixed with 8 parts of the mixture and stirred continuously. 15 parts of the delivery system were slowly added and emulsified and sheared at 10,000 rpm for 15 min to obtain the brain-boosting and intelligence-enhancing multi-component composition. Example 6

[0028] A multi-component composition for brain health and intelligence enhancement, and its preparation method: S1. Peptide preparation: 5 parts flaxseed powder and 2 parts salmon protein were mixed, 70 parts water were added and stirred evenly, 1.5 parts neutral protease, 0.5 parts alkaline protease and 0.5 parts trypsin were added, and the mixture was kept at 35℃ and stirred at 200 rpm for 3 hours for enzymatic hydrolysis. After completion, the temperature was raised to 75℃ to inactivate the enzyme for 15 minutes, cooled, and centrifuged at 8000 rpm for 15 minutes to collect the supernatant. The supernatant was then double filtered through an ultrafiltration membrane to retain peptides of 1000-3000 Da. The peptide solution was collected and freeze-dried to obtain flaxseed-salmon enzymatic hydrolysed peptide powder. S2. Preparation of Chinese herbal extract: 9 parts of Alpinia oxyphylla, 6 parts of Gastrodia elata, and 7 parts of Ziziphus jujuba var. spinosa were pulverized and passed through a 60-mesh sieve to obtain a mixed powder. 135 parts of water were added and flash extraction was performed at a speed of 4000 rpm for 10 min. After the extraction was completed, the supernatant A was collected by centrifugation. Five times the amount of ethanol solution was added to the extracted residue, and flash extraction was performed again. After the extraction was completed, the supernatant B was collected by centrifugation. Supernatant A and supernatant B were mixed and rotary evaporated to obtain the Chinese herbal extract. S3. Extraction of milk fat globule membrane: The light cream separated from raw milk was washed three times with phosphate buffer at pH 6.5, centrifuged at 8000 rpm for 10 min to obtain milk fat globules, and the washed phosphate buffer was collected and refrigerated for later use. The milk fat globules were sonicated at 500W for 15 min with a working time of 5 s and an interval of 3 s. The lower layer mixture was collected by centrifugation, concentrated, and freeze-dried to obtain milk fat globule membrane powder. S4. Preparation of colostrum: 4 parts of Chinese herbal extract and 0.5 parts of epigallocatechin gallate were added to 80 parts of water and stirred evenly. 2 parts of flaxseed-salmon enzymatic hydrolysate powder were added and homogenized at 8 MPa to obtain an aqueous solution. 4 parts of lecithin, 2 parts of phosphatidylserine, and 1 part of N-acetylneuraminic acid were added to 50 parts of anhydrous ethanol and stirred at a constant temperature of 35°C in a water bath to obtain a composite phospholipid solution. 3 parts of the aqueous solution were slowly added dropwise to 3 parts of the composite phospholipid solution and sheared at 8000 rpm for 10 min to form the colostrum. S5. Preparation of delivery system: 8 parts of primary emulsion and 8 parts of 2 wt.% milk fat globule membrane solution were mixed, ultrasonically cavitated at 300W for 10 min, and then stirred at 35℃ for 30 min to obtain emulsion. Pig blood extract was dissolved in phosphate buffer to obtain a 3 wt.% modification solution. 15 parts of emulsion and 3 parts of modification solution were mixed, stirred and incubated at 100 rpm for 3 h, homogenized at 20 MPa, and then filtered through a microporous membrane to obtain the delivery system. S6. Preparation of multi-component composition: 2 parts of Armillaria mellea polysaccharide and 8 parts of washed phosphate buffer were mixed evenly and stirred in a 40°C water bath at 500 rpm for 20 min to obtain a mixture. 1 part of kudzu root powder was heated with 9 parts of water to gelatinize, and stirring was continued during the process. 1 part of gelatinized kudzu root powder was mixed with 5 parts of the mixture and stirred continuously. 10 parts of the delivery system were slowly added and emulsified and sheared at 10,000 rpm for 15 min to obtain the brain-boosting and intelligence-enhancing multi-component composition. Comparative Example 1

[0029] The difference between this comparative example and Example 1 is that no compound protease was added to hydrolyze flaxseed powder and salmon protein; the remaining steps are the same as in Example 1. Comparative Example 2

[0030] The difference between this comparative example and Example 1 is that the molecular weight of the polypeptide solution is 3000-5000 Da, while the other steps are the same as in Example 1. Comparative Example 3

[0031] The difference between this comparative example and Example 1 is that milk fat globule membrane is not added; the remaining steps are the same as in Example 1. Comparative Example 4

[0032] The difference between this comparative example and Example 1 is that the modified solution is not prepared by adding pig blood extract; the remaining steps are the same as in Example 1. Comparative Example 5

[0033] The difference between this comparative example and Example 1 is that lecithin, phosphatidylserine, and N-acetylneuraminic acid are not added; the remaining steps are the same as in Example 1. Comparative Example 6

[0034] The difference between this comparative example and Example 1 is that Armillaria mellea polysaccharide is not added; the remaining steps are the same as in Example 1. Comparative Example 7

[0035] The difference between this comparative example and Example 1 is that phosphate buffer is used instead of the phosphate buffer used for washing during the preparation of milk fat globule membranes; the other steps are the same as in Example 1. Indicator Test

[0036] Sensory evaluation was conducted on the multi-component brain-boosting and intelligence-enhancing oral liquid, with a maximum score of 100. Twenty volunteers tasted the multi-component brain-boosting and intelligence-enhancing oral liquid.

[0037] The results are shown in Table 1. Example 6 scored the highest, indicating the best sensory quality and the highest acceptability. In summary, all examples scored above 90 points, higher than the comparative examples. The overall performance was characterized by uniform color, delicate texture, and good bitterness masking. The core reason for this was that the examples used Armillaria mellea polysaccharide, milk fat globule membrane washing buffer, and kudzu root powder to adjust the texture and mask bitterness, and the process parameters were optimized to ensure the sensory quality of the product. Comparative Example 1 lacked an enzymatic hydrolysis process and contained protein particles, which may have resulted in a less delicate texture. Comparative Examples 6 and 7, lacking Armillaria mellea polysaccharide and milk fat globule membrane washing buffer, showed the most significant score drops, indicating poorer flavor and texture.

[0038] Table 1. Sensory evaluation results of the multi-component brain-boosting and intelligence-enhancing composition.

[0039] Component testing After sample pretreatment, the results were measured and calculated using high performance liquid chromatography (HPLC) with a UV detector.

[0040] Gastrodin, the core active ingredient of Gastrodia elata, improves cerebral circulation, protects nerve cells, and enhances learning and memory abilities. α-Linolenic acid, the core unsaturated fatty acid of flaxseed, is an important raw material for the synthesis of brain nerve cells and helps repair nerve membranes. Phosphatidylserine can directly participate in the construction of nerve cell membranes, promote nerve signal transmission, and improve brain fatigue. Alpinia oxyphylla is a characteristic active ingredient of Alpinia oxyphylla, which has the effects of improving intelligence, calming the mind, and enhancing cognitive function. The content of these functional components in the composition is shown in Table 2. All components in Comparative Example 3 were reduced because the milk fat globule membrane can provide some protection through encapsulation; loss of the milk fat globule membrane leads to the loss of active ingredients during preparation. The α-linolenic acid content in Comparative Example 1 was significantly reduced because the enzymatic hydrolysis process can destroy the flaxseed cell wall and release α-linolenic acid; Comparative Example 1 lacked the enzymatic hydrolysis process. The phosphatidylserine content in Comparative Example 5 was extremely low. The components in Comparative Examples 6 and 7 were slightly reduced because Armillaria mellea polysaccharide and milk fat globule membrane washing buffer are the final stabilizers in the product; their absence leads to decreased encapsulation of the delivery system and the loss of a small amount of active ingredients.

[0041] Table 2. Content of functional components in the brain-boosting and intelligence-enhancing composition.

[0042] Brain-boosting and intelligence-enhancing efficacy test SPF-grade male ICR mice, weighing 22-25g, were selected and acclimatized for one week before being randomly divided into a blank control group, a model group, and an experimental group, with 10 mice in each group. Except for the blank control group, all other groups received intraperitoneal injections of scopolamine 3mg / kg to establish a cognitive impairment model, once daily for 7 consecutive days. Simultaneously, the drug-treated groups were administered the corresponding drug via gavage, while the blank control group and the model group received an equal volume of physiological saline via gavage for 14 consecutive days. Cognitive function was assessed using the Morris water maze test. After the last drug administration, mice underwent orientation and navigation training (day 4) and a spatial exploration test (day 5). Brain tissue was collected after the experiment to detect AChE and BDNF levels.

[0043] Table 3 shows the animal efficacy evaluation results. Example 5 had the closest pharmacodynamic data to the control group, with the shortest escape latency (26.8s) and the highest BDNF content (17.2 ng / mL), consistent with the trend of functional component content, proving a positive correlation between component content and brain-boosting effects. All examples showed AChE activity close to the control group, and BDNF content >15 ng / mL, indicating that the product can effectively inhibit cholinesterase activity, increase neurotrophic factor content, and improve cognitive impairment. Comparative Examples 3 and 4 had the worst data, close to the model group. The absence of milk fat globule membrane and pig blood extract, the core of the delivery system, prevented the active ingredients from efficiently penetrating the blood-brain barrier, resulting in insufficient concentration of effective components in the brain and poor cognitive improvement. Comparative Examples 1 and 2, due to the lack of enzymatic hydrolysis and the excessively large molecular weight of the peptides, had low absorption efficiency of components such as α-linolenic acid, preventing brain nerve cells from effectively obtaining nutrients and resulting in poor improvement in learning and memory abilities. The pharmacodynamic data of all embodiments were significantly better than those of the comparative examples and closer to those of the blank group; the indicators of all comparative examples were better than those of the model group, indicating that even if some raw materials and processes are missing, the product still has a certain brain-boosting effect. However, core raw materials such as milk fat globule membrane, pig blood extract and key processes are necessary conditions for the product to exert its efficient brain-boosting effect.

[0044] Table 3. Results of Animal Efficacy Evaluation

[0045] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Any person skilled in the art can make many possible variations and modifications to the technical solutions of the present invention, or modify them into equivalent embodiments, without departing from the spirit and technical essence of the present invention. Therefore, any simple modifications, equivalent substitutions, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention, without departing from the content of the technical solutions of the present invention, shall still fall within the scope of protection of the present invention.

Claims

1. A method for preparing a multi-component composition for improving brain function and intelligence, characterized in that, Includes the following steps: S1. Peptide preparation: Flaxseed powder and salmon protein were mixed, water was added and stirred evenly, a complex protease was added, and the mixture was stirred at a constant temperature for enzymatic hydrolysis. After completion, the enzyme was inactivated by heating, cooled, centrifuged, and the supernatant was collected and filtered twice through an ultrafiltration membrane. The peptide solution was collected and freeze-dried to obtain flaxseed-salmon enzymatic hydrolysed peptide powder. S2. Preparation of Chinese herbal extract: Alpinia oxyphylla, Gastrodia elata, and Ziziphus jujuba seeds were pulverized and sieved to obtain mixed powder. Water was added and flash extraction was performed. After the extraction was completed, the supernatant A was collected by centrifugation. Ethanol solution was added to the extracted residue and flash extraction was performed again. After the extraction was completed, the supernatant B was collected by centrifugation. Supernatant A and supernatant B were mixed and rotary evaporated to obtain Chinese herbal extract. S3. Extraction of milk fat globule membrane: The light cream separated from raw milk is repeatedly washed and centrifuged with phosphate buffer to obtain milk fat globules. The washed phosphate buffer is collected and refrigerated for later use. The milk fat globules are ultrasonically broken up and centrifuged. The lower layer mixture is collected, concentrated, and freeze-dried to obtain milk fat globule membrane powder. S4. Preparation of colostrum: The extract of traditional Chinese medicine and epigallocatechin gallate were added to water and stirred evenly. Flaxseed-salmon enzymatic hydrolysate powder was added and homogenized to obtain an aqueous solution. Lecithin, phosphatidylserine and N-acetylneuraminic acid were added to anhydrous ethanol and stirred in a water bath at a constant temperature to obtain a composite phospholipid solution. The aqueous solution was slowly added dropwise to the composite phospholipid solution and high-speed shearing was used to form a colostrum. S5. Preparation of delivery system: The primary emulsion and milk fat globule membrane solution were mixed, ultrasonically cavitated, and stirred at a constant temperature to obtain an emulsion. The pig blood extract was dissolved in phosphate buffer to obtain a modification solution. The emulsion and modification solution were mixed, stirred and incubated, homogenized, and then filtered through a microporous membrane to obtain the delivery system. S6. Preparation of multi-component composition: The polysaccharide of Armillaria mellea is mixed evenly with the washed phosphate buffer solution and stirred in a water bath to obtain a mixture. The kudzu root powder is heated with water to gelatinize, and stirred continuously during the process. The gelatinized kudzu root powder is mixed with the mixture and stirred continuously. The mixture is then slowly added to the delivery system, emulsified and sheared to obtain the brain-boosting and intelligence-enhancing multi-component composition.

2. The method for preparing a brain-boosting and intelligence-enhancing multi-component composition according to claim 1, characterized in that: In step S1, the mass ratio of flaxseed powder, salmon protein, and water is (5-8):(2-4):(70-100); the complex protease includes neutral protease, alkaline protease, and trypsin, and the amount of complex protease added is 3-5 wt.%; the constant temperature stirring temperature is 35-45℃, the stirring speed is 200-300 rpm, and the time is 3-4 h; the heating temperature is 75-85℃, and the enzyme inactivation time is 15-25 min; the centrifugation speed is 7000-8000 rpm, and the time is 10-15 min; the molecular weight of the polypeptide solution is 1000-3000 Da.

3. The method for preparing a brain-boosting and intelligence-enhancing multi-component composition according to claim 2, characterized in that: The mass ratio of the neutral protease, alkaline protease, and trypsin is (2-3):(1-2):(1-2).

4. The method for preparing a brain-boosting and intelligence-enhancing multi-component composition according to claim 1, characterized in that: In step S2, the mass ratio of Alpinia oxyphylla, Gastrodia elata, and Ziziphus jujuba var. spinosa is (6-16):(5-15):(7-18), and the mass ratio of the mixed medicinal powder to water is 1:(6-8); the mass ratio of the residue to the ethanol solution is 1:(5-7), and the concentration of the ethanol solution is 70-85%; the flash extraction speed is 3000-5000 rpm, and the time is 5-10 min.

5. The method for preparing a brain-boosting and intelligence-enhancing multi-component composition according to claim 1, characterized in that: In step S3, the pH of the phosphate buffer solution is 6.5-7.5; washing is performed 2-3 times; centrifugation is performed at 8000-9000 rpm for 10-15 min; ultrasonic disruption is performed at 300-500 W for 5 s of ultrasonic operation time, with a 3 s interval, for a total ultrasonic time of 10-15 min.

6. The method for preparing a brain-boosting and intelligence-enhancing multi-component composition according to claim 1, characterized in that: In step S4, the mass ratio of the herbal extract, epigallocatechin gallate, flaxseed-salmon enzymatic hydrolysate powder, and water is (3-4):(0.2-0.5):(2-3):(80-100); the mass ratio of lecithin, phosphatidylserine, N-acetylneuraminic acid, and anhydrous ethanol is (8-10):(3-5):(2-3):(100-120); the mass ratio of the composite phospholipid solution to the aqueous phase is (1-3):1; the homogenization pressure is 5-10 MPa; the high-speed shearing speed is 8000-9000 rpm, and the time is 5-10 min.

7. The method for preparing a brain-boosting and intelligence-enhancing multi-component composition according to claim 1, characterized in that: In step S5, the concentration of the milk fat globule membrane solution is 1-2 wt.%; the pH of the phosphate buffer is 7-7.5; the concentration of the porcine blood extract in the modification solution is 2-5 wt.%; the mass ratio of the primary emulsion to the milk fat globule membrane solution is (1-2):(1-2); the mass ratio of the emulsion to the modification solution is (3-5):1; the ultrasonic power is 200-300W, and the ultrasonic time is 10-15 min; the constant temperature stirring temperature is 30-35℃, and the time is 30-50 min; the stirring speed is 100-200 rpm; the incubation time is 2-3 h; and the homogenization pressure is 20-25 MPa.

8. The method for preparing a brain-boosting and intelligence-enhancing multi-component composition according to claim 1, characterized in that: In step S6, the mass ratio of Armillaria mellea polysaccharide to washed phosphate buffer is (1-2):(8-9); the water bath stirring temperature is 40-50℃, the stirring speed is 300-500rpm, and the time is 20-30min; the mass ratio of kudzu root powder to water is 1:(8-10); the mass ratio of gelatinized kudzu root powder, mixture, and delivery system is 1:(5-8):(10-15); the emulsification shearing speed is 9000-10000rpm, and the time is 15-20min.

9. A brain-boosting and intelligence-enhancing multi-component composition prepared according to the preparation method described in claims 1-8.

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