A composition for improving post-exercise fatigue and use thereof

Abstract

The present application relates to a kind of compositions for improving post-exercise fatigue and its application, belong to functional food technical field.The composition includes collagen peptide and beer yeast peptide, the mass ratio of the two is 3-14:1, preferably 3-6:1, and active peptide ingredients such as casein peptide, elastin peptide, oyster peptide, sea cucumber peptide and the like can be further added, and active protein such as non-denatured type II collagen, concentrated whey protein, instant soybean powder.Animal experiment results show that the composition can significantly prolong exercise endurance time, reduce lactic acid and urea nitrogen level, improve liver and skeletal muscle glycogen reserves, enhance antioxidant enzyme (SOD, GSH-Px) activity, reduce lipid peroxide (MDA) and inflammatory factor (IL-6, TNF-α, IL-1β) expression, with multi-mechanism synergistic function to improve post-exercise fatigue.The present application composition is suitable for preparing post-exercise recovery type functional food, and has good application prospect.

Description

Technical Field

[0001] This invention relates to the field of sports nutrition and functional food technology, specifically to a protein peptide composition for improving post-exercise fatigue and its application in sports nutrition foods. Background Technology

[0002] With the popularization of fitness for all and competitive sports, post-exercise fatigue has gradually attracted attention. During exercise, the body consumes a large amount of energy, accompanied by the accumulation of metabolic products such as lactic acid and an increase in oxidative stress levels. If recovery is not timely, it can easily lead to a decline in athletic performance and even sports injuries. Therefore, how to improve post-exercise fatigue and promote recovery through reasonable nutritional supplementation has become an important research direction in the field of sports nutrition.

[0003] In existing technologies, nutritional interventions for post-exercise fatigue mainly include supplementing carbohydrates, proteins, and their hydrolysates. Among these, proteins and peptides are widely used in sports nutrition foods due to their high nutritional value and good absorption properties. For example, whey protein, soy protein, collagen, and their hydrolyzed peptides are often used in post-exercise recovery products to supplement amino acids, promote tissue repair, and maintain normal metabolism.

[0004] However, existing protein or single-source peptide preparations still have certain shortcomings in improving post-exercise fatigue. On the one hand, some proteins have large molecular weights and limited digestion and absorption rates, making it difficult to meet the body's need for rapid nutritional replenishment after exercise. On the other hand, protein peptides from different sources differ in amino acid composition, molecular weight distribution, and physiological functions, and a single peptide source often cannot simultaneously achieve ideal effects in multiple aspects such as enhancing antioxidant capacity and regulating energy metabolism.

[0005] In addition, while some existing technologies aim to improve post-exercise fatigue by combining multiple nutrients, they lack a systematic consideration of the synergistic effects between different peptides in their formulation design, or fail to reasonably limit the proportion range and molecular weight characteristics of key peptides. This results in unstable product efficacy and makes it difficult to achieve a balance between improving post-exercise fatigue, enhancing exercise endurance, and promoting post-exercise recovery.

[0006] Therefore, there is an urgent need for a new technical solution that, while ensuring good nutritional supplementation effects, improves the utilization efficiency of small molecule peptides through the rational selection and ratio of protein peptides from different sources, thereby more effectively improving post-exercise fatigue and meeting the functional and stability requirements of sports nutrition foods in practical applications. In view of this, the purpose of this invention is to provide a composition for improving post-exercise fatigue and its application. By rationally selecting and combining protein peptide components from different sources, the absorption and utilization efficiency of post-exercise nutritional supplementation is improved, thereby improving the body's fatigue state after exercise and helping to enhance exercise endurance and promote post-exercise recovery. Summary of the Invention

[0007] To achieve the above objectives, the present invention provides the following technical solution: The present invention provides a composition for improving post-exercise fatigue, wherein the composition uses collagen peptides and brewer's yeast peptides as core functional components, and reasonably limits the mass ratio, peptide characteristics, and optional additional components of the two; at the same time, the present invention also provides the application of the composition in sports nutrition foods and related products.

[0008] In one embodiment of the present invention, a composition for improving post-exercise fatigue is provided, the composition comprising collagen peptides and brewer's yeast peptides, wherein the mass ratio of the collagen peptides to the brewer's yeast peptides is 3-14:1.

[0009] Furthermore, the mass ratio of the collagen peptide to the brewer's yeast peptide is 3-6:1.

[0010] Preferably, the mass ratio of collagen peptide to brewer's yeast peptide is 3:1.

[0011] Furthermore, the brewer's yeast peptide contains peptide segments with amino acid sequences of LGGPLL and LGPTGISM, and the content of peptide segments with a molecular weight of less than 1000 Da accounts for more than 50% of the total mass of the brewer's yeast peptide.

[0012] Optionally, the composition may further comprise one or more additional active peptides selected from casein peptides, elastin peptides, oyster peptides, and sea cucumber peptides.

[0013] Optionally, the composition may further comprise one or more active proteins selected from non-denatured type II collagen, whey protein concentrate, and instant soy flour.

[0014] Optionally, the composition may further comprise one or more components selected from polydextrose, theanine, and olive fruit powder.

[0015] Further, by weight, the composition comprises 45-50 parts of collagen peptides, 3.5-14 parts of brewer's yeast peptides, and a total of 35-50 parts of other components, wherein the other components include excipients and / or flavoring agents and / or functional ingredients; wherein the weight parts correspond to the mass ratio of collagen peptides to brewer's yeast peptides, and the mass ratio of collagen peptides to brewer's yeast peptides is 3-14:1.

[0016] In one embodiment of the present invention, a sports nutrition food is provided, the sports nutrition food comprising the above-described composition for improving post-exercise fatigue.

[0017] In one embodiment of the present invention, the composition is used to prepare a product for improving post-exercise fatigue, and / or enhancing exercise endurance, and / or promoting post-exercise recovery.

[0018] Furthermore, the improvement in post-exercise fatigue is achieved by enhancing the body's antioxidant capacity.

[0019] Furthermore, the improvement of post-exercise fatigue is achieved by regulating post-exercise energy metabolism.

[0020] Based on the above technical solution, the present invention provides a composition for improving post-exercise fatigue and its application. This composition uses collagen peptides and brewer's yeast peptides as core functional ingredients. Through a rational setting of the mass ratio of these two components, the resulting composition possesses both high protein and peptide nutritional value and meets the body's need for rapid absorption and utilization after exercise.

[0021] Specifically, this invention introduces brewer's yeast peptides from a specific source and with a specific molecular weight distribution, and synergistically combines them with collagen peptides. This results in a higher proportion of small-molecule peptides in the composition, which facilitates rapid absorption by the body after exercise, thereby providing the body with essential amino acids and energy metabolism substrates. Simultaneously, by limiting the ratio range of collagen peptides to brewer's yeast peptides, a relative balance is achieved between the two in terms of nutritional supplementation and functional synergy, contributing to a better overall effect of the composition in alleviating post-exercise fatigue.

[0022] Based on the above, the composition of the present invention may further include additional active peptides, active proteins and other functional components as needed, so that while maintaining the core anti-fatigue effect, it also meets the requirements of sports nutrition foods in terms of taste, formula stability and comprehensive nutrition, thereby improving the applicability and scalability of the product in practical applications.

[0023] Through the above technical solutions, when the composition of the present invention is applied to sports nutrition foods or related products, it can reduce the degree of fatigue after exercise by improving the body's antioxidant capacity and / or regulating the energy metabolism state after exercise, promote the recovery of physical function after exercise, and help to enhance exercise endurance to a certain extent. It is suitable for the preparation of sports nutrition products for post-exercise recovery and protein supplementation. Attached Figure Description

[0024] Figure 1 This is a statistical diagram illustrating the effect of the composition of the present invention on the time to exhaustion during weight-bearing swimming in a mouse model of post-exercise fatigue, used to explain the effect of the composition on improving exercise endurance; Figure 2 This is a schematic diagram illustrating the changes in lactic acid and urea nitrogen levels in mouse blood after intervention with the composition of the present invention, used to explain the regulatory effect of the composition on energy metabolism after exercise. Figure 3 This is a schematic diagram illustrating the changes in glycogen content in mouse skeletal muscle and liver after intervention with the composition of the present invention, used to explain the effect of the composition on the body's energy reserves; Figure 4 This is a schematic diagram illustrating the changes in serum levels of inflammatory factors IL-6, TNF-α, and IL-1β in mice after intervention with the composition of the present invention, used to illustrate the anti-inflammatory effects of the composition. Detailed Implementation

[0025] To further verify the functional effects of the collagen peptide and brewer's yeast peptide composition proposed in this invention on improving post-exercise fatigue and to clarify its physiological regulatory mechanism in vivo, this invention constructs an animal experimental model and conducts systematic research on key indicators such as exercise endurance, energy metabolism, oxidative stress, and inflammatory response. The following examples, in conjunction with other embodiments, provide a detailed description of the specific implementation process, parameter selection, and experimental results of the technical solution of this invention, so that those skilled in the art can clearly understand and implement the technical content proposed in this invention.

[0026] I. Establishment of Animal Experimental Models and Group Design This embodiment constructs a mouse model of post-exercise fatigue and intervenes with different doses of the functional composition to systematically evaluate the physiological effects of the composition in improving post-exercise fatigue.

[0027] (1) Selection and feeding conditions of experimental animals Healthy male C57BL / 6J mice were used in the experiment and purchased from a legally qualified laboratory animal supplier, specifically Speford (Beijing) Biotechnology Co., Ltd. The mice were 6–8 weeks old and weighed between 16.12–17.12 g. Prior to the experiment, all mice underwent 7 days of acclimatization rearing in an SPF-grade (clean) animal facility, with the following environmental controls implemented: Temperature: 22 ± 2℃; Humidity: 50% ± 10%; Irradiance cycle: 12 hours of light / 12 hours of darkness; Feeding method: Free access to standard feed and water; The animals are in good health, excluding individuals that are sick, malnourished, or exhibit abnormal behavior.

[0028] (2) Experimental group design After the adaptation period, the mice were randomly divided into 5 groups based on their weight and health status, with 15 animals in each group, 3 cages per group, and 5 animals in each cage. The specific grouping is as follows: Negative control group (model group, no intervention was given, only an exercise fatigue model was established). Positive control group (model group, administered oyster peptides known to have anti-fatigue effects by gavage). Low-dose experimental group (model group, administered the composition of the present invention by gavage, with a collagen peptide to brewer's yeast peptide mass ratio of 28:1). Medium-dose experimental group (model group, administered the composition of the present invention by gavage at a mass ratio of 14:1). High-dose experimental group (model group, administered the composition of the present invention by gavage at a mass ratio of 3:1).

[0029] (3) Intervention method and dosage The intervention period was set at 42 consecutive days. The experimental group received a combination of collagen peptides and brewer's yeast peptides in appropriate proportions via gavage. The positive control group received oyster peptides via gavage, and the negative control group received an equal volume of distilled water via gavage. The gavage parameters were as follows: Composition preparation: 5.0 g of the composition of the present invention was dissolved in 35 mL of deionized water to form a homogeneous solution; Oral administration dosage: 0.2 mL per mouse per day, once a day; Positive control group: 2.0 g of commercially available oyster peptide powder was dissolved in 14 mL of water and administered by gavage in the same proportion; Negative control group: Only 0.2 mL of deionized water was given.

[0030] During the intervention, the following parameters were recorded regularly each week: Changes in mouse body weight; Dietary and water intake; Activity status (e.g., whether active or sleepy); Wool luster; Whether any obvious adverse reactions occur (such as diarrhea, aggressive behavior, etc.); Dead or abnormal individuals should be promptly removed from the records.

[0031] Throughout the experiment, the operators strictly adhered to ethical guidelines for laboratory animals to ensure animal welfare. The final gavage was administered within 30 minutes of the end of the experiment, followed by the exhaustive swimming test phase (see the subsequent implementation section for details).

[0032] II. Methods and Results for Evaluating Exercise Endurance in Mice (corresponding) Figure 1 ) To evaluate the effect of the composition of the present invention on post-exercise fatigue recovery, a mouse weight-bearing forced swimming model was constructed, and the exercise endurance of mice in each experimental group was functionally tested.

[0033] (1) Test method On day 42 of the experiment, 30 minutes after the final gavage, the mice in each group were tested for endurance using a weighted forced swimming method. The test parameters and procedures are as follows: Test equipment: homemade swimming tub (30 cm deep, with an inner diameter sufficient to prevent mice from climbing), water temperature controlled at 25 ± 1℃; Weight setting: Use medical tape to fix lead sheet weighing approximately 5% of the mouse's body weight to the end of the mouse's tail; Testing process: The mouse was placed alone in the bucket of water, and the timer was started. Swimming until you can no longer keep your head above water, your body sinks, and you do not struggle for 10 seconds is considered "exhaustion"; Record the swimming time (in seconds) from entry into the water to exhaustion, and provide immediate assistance.

[0034] All experiments were conducted under identical environmental conditions and performed by the same operator to minimize human bias. Each mouse was tested only once.

[0035] (2) Analysis of experimental results Experimental results are as follows Figure 1 As shown: The mice in the negative control group had the shortest average time to swim to exhaustion, indicating that the body's fatigue recovery ability was poor under the simple exercise model condition; The swimming time of the positive control group was significantly longer than that of the negative control group by 54.9% (P<0.0001), which verified the known anti-fatigue effect of oyster peptides; The swimming time in the low-dose experimental group was 16.0% longer than that in the negative control group (P = 0.0051). The medium-dose experimental group had a 22.1% longer duration of action (P = 0.0015). The high-dose experimental group prolonged the duration of the disease by 15.6% (P = 0.0398). The above results indicate that the collagen peptide-brewer's yeast peptide composition of the present invention has a good effect on improving exercise endurance, especially under medium dose conditions, and shows a certain dose-dependent trend.

[0036] Therefore, the functional composition provided by the present invention can effectively improve the body's tolerance time under exercise load, delay the onset of fatigue, and has good potential for post-exercise recovery support.

[0037] III. Detection methods and results of metabolites in blood (corresponding) Figure 2 ) To further evaluate the effects of the composition of the present invention on post-exercise fatigue metabolism-related indicators, the effects of lactic acid (LA) and blood urea nitrogen (BUN) levels in mouse serum on the clearance of metabolic waste after exercise were analyzed.

[0038] (1) Detection method Immediately after the swimming exhaustion experiment, blood was drawn from the eyes of the mice. The specific steps are as follows: Blood collection time: Perform the procedure immediately after exhaustion to ensure timely sample collection; Processing flow: After collecting whole blood, let it stand at room temperature for 30 minutes; Separate the serum by centrifugation at 3000 rpm for 15 minutes; Detection indicators and methods: Serum lactate (LA) levels; Serum blood urea nitrogen (BUN) levels; All reagent kits used were purchased from qualified third-party testing suppliers and operated in accordance with the instructions. All samples were set up with 3 replicate wells, and the final result was the average value.

[0039] (2) Analysis of experimental results The results are as follows Figure 2 As shown, this is mainly reflected in the following aspects: Changes in lactic acid (LA) content: Compared with the negative control group, the lactate level in the positive control group decreased significantly by 29.2% (P<0.0001). In the experimental groups, lactate levels decreased by 14.0% in the low-dose group and 8.4% in the medium-dose group. Although the high-dose group showed a decreasing trend, the statistical difference was not significant. Overall, all experimental groups showed a trend of lactic acid clearance.

[0040] Changes in blood urea nitrogen (BUN) levels: Elevated BUN levels usually indicate glycogen depletion and increased protein mobilization during exercise. Both the positive control group and each experimental group showed a decrease in BUN levels; Compared with the negative control group, the low, medium, and high dose experimental groups showed a decrease of 9.7%, 15.8%, and 14.2%, respectively. The medium dose group showed the best effect, suggesting that it has a regulatory effect on abnormal protein metabolism.

[0041] (3) Summary of results The above results indicate that the composition of the present invention can effectively promote the clearance of lactic acid and urea nitrogen after exercise, thereby reducing the aggravating effect of metabolic product accumulation on physical fatigue. Among them, the high-dose group showed a more significant trend in clearing lactic acid, while the medium-dose group performed better in regulating urea nitrogen levels, indicating that the composition has multiple pathways of action in regulating energy metabolism and alleviating the accumulation of fatigue substances.

[0042] These results further corroborate the potential of this composition to improve post-exercise metabolic disorders and accelerate fatigue recovery, providing experimental evidence for its use as a functional sports nutrition ingredient.

[0043] IV. Glycogen Reserve Detection Methods and Results (corresponding) Figure 3 ) Glycogen is an important energy source during physical activity, and its reserve level directly affects exercise endurance and fatigue recovery. This embodiment evaluates the effect of the composition of the present invention on improving energy reserve capacity by measuring the glycogen content in the gastrocnemius muscle and liver tissue of mice.

[0044] (1) Detection and processing methods After the experiment, the mice were euthanized by cervical dislocation, and the gastrocnemius muscle and liver tissue were quickly dissected to determine the glycogen content.

[0045] Material source: Gastrocnemius muscle: Represents skeletal muscle tissue and is directly involved in movement; The liver, as the core organ for glycogen storage and metabolism, reflects the overall energy regulation capacity.

[0046] Processing flow: Take approximately 100 mg of each tissue and homogenize it with PBS buffer at a mass-to-volume ratio of 1:9. The supernatant of the homogenized sample was collected by high-speed centrifugation. Using a commercially available biochemical assay kit for glycogen content, and following the instructions, tissue glycogen concentration was determined by colorimetric method. Three parallel wells were set up for each sample, and the average value was taken as the final result.

[0047] (2) Analysis of experimental results Test results as follows Figure 3 As shown: Glycogen content of gastrocnemius muscle: The muscle glycogen content in the positive control group was significantly increased by 2.15 times compared with the negative control group (P<0.0001). The low-dose group showed a significant increase of 1.41 times (P < 0.0001). The medium-dose group showed a 1.83-fold increase (P < 0.0001). The high-dose group showed a 1.96-fold increase (P < 0.0001).

[0048] Liver glycogen content: The positive group showed a significant increase; The low-dose group showed a 1.29-fold increase (P < 0.0001). The medium-dose group showed a 1.47-fold increase (P < 0.0001). The high-dose group showed a 1.66-fold increase (P < 0.0001). All experimental groups showed a clear dose-dependent trend.

[0049] (3) Summary of results The results of this experiment demonstrate that the composition described in this invention can effectively promote the synthesis and storage of glycogen in skeletal muscle and liver tissue, significantly enhancing the body's energy reserve capacity. Increased glycogen content means a more sufficient energy supply during exercise, thereby helping to delay fatigue and accelerate post-exercise recovery.

[0050] All dosage groups showed good effects, especially the medium and high dosage groups, where the glycogen increase was close to or exceeded that of the positive group. This indicates that the composition of the present invention has a significant advantage in promoting glycogen synthesis and exhibits a clear dose-dependent effect, providing strong data support for its functional effects.

[0051] V. Evaluation methods and results of antioxidant capacity (see Table 1) Exercise triggers significant oxidative stress, leading to the generation of large amounts of reactive oxygen species (ROS), which damage cell membranes, disrupt metabolic balance, and exacerbate exercise-induced fatigue. Therefore, this embodiment evaluates the antioxidant regulatory capacity of the composition of the present invention by detecting typical antioxidant enzyme activity indicators and lipid peroxidation product levels in serum and gastrocnemius muscle.

[0052] (1) Detection indicators and sample sources Testing indicators: SOD (superoxide dismutase) activity: represents the ability to scavenge superoxide anions; GSH-Px (glutathione peroxidase) activity: reflects the ability to scavenge ROS such as hydrogen peroxide; MDA (malondialdehyde) content: an end product of lipid peroxidation, reflecting the degree of oxidative damage to tissues.

[0053] Sample source: serum; Gastrocnemius muscle tissue homogenate (tissue:PBS buffer = 1:9).

[0054] (2) Detection method All tests were performed using commercially available colorimetric reagent kits (purchased from Jiangsu Aidisheng Biotechnology Co., Ltd.), following the standard operating procedures outlined in the instructions. Detection wavelength setting: SOD: 550 nm; GSH-Px: 412 nm; MDA: 532 nm; Three parallel wells were set for each sample, and the average value was taken as the final detection result.

[0055] (3) Analysis of experimental results The experimental data are shown in Table 1, and the main results are as follows: Serum markers: MDA content: The negative control group had a concentration of 7.62 ± 0.73 nmol / L. The high-dose group showed a significant decrease to 4.45 ± 0.54 nmol / L (a decrease of 41.6%, P < 0.0001). The low- and medium-dose groups showed a decrease of 20.7% (P=0.0018) and 15.6% (P=0.0287), respectively. SOD activity: The negative control group had a concentration of 35.51 ± 3.22 U / mL. The high-dose group increased to 44.82 ± 10.02 U / mL (an increase of 26.2%, P=0.03); GSH-Px activity: The negative group was 271.31 ± 35.27 U / mL; The high-dose group increased to 548.42 ± 37.08 U / mL (an increase of 102.1%, P < 0.0001); The medium and low dose groups showed increases of 80.5% and 45.0%, respectively.

[0056] Gastrocnemius muscle index: The MDA level in the high-dose group was slightly higher than that in the control group, while there were no significant differences among the other groups. The SOD activity in each experimental group was higher than that in the negative control group (P<0.05). GSH-Px activity was significantly increased in all groups, especially in the positive group (↑122%) and the high-dose group (↑92.8%).

[0057] Table 1 Antioxidant Capacity Test

[0058] (4) Summary of results The results of this experiment show that the composition described in this invention can significantly: Enhance the activity of antioxidant enzyme systems (SOD, GSH-Px); Reduce the content of oxidative stress products (MDA); Enhance the tissue's ability to defend against oxidative damage.

[0059] In particular, the high-dose group exhibited the best antioxidant regulatory effect in serum, indicating that the composition can effectively alleviate exercise-induced oxidative stress and has a significant effect on maintaining cellular metabolic balance and delaying the onset of fatigue. This mechanism of action complements its overall function of improving post-exercise fatigue, supporting its practical application value in the field of sports nutrition.

[0060] VI. Methods and Results for Detecting Inflammatory Factors (corresponding) Figure 4 ) After strenuous or high-intensity exercise, the body often experiences varying degrees of inflammatory response, manifested as elevated levels of inflammatory factors. This not only affects muscle repair and metabolic function but also delays the fatigue recovery process. Therefore, this embodiment detects the expression levels of typical pro-inflammatory factors in mouse serum to analyze the intervention ability of the composition of this invention against exercise-induced inflammation.

[0061] (1) Detection indicators and methods Testing indicators: Interleukin-6 (IL-6); Tumor necrosis factor α (TNF-α); Interleukin-1β (IL-1β).

[0062] Sample source: Mouse serum samples were collected after the experiment, frozen at -80℃, thawed and centrifuged to remove impurities before testing.

[0063] Detection method: A mouse-specific ELISA kit was used (purchased from Jiangsu Sumeke Biotechnology Co., Ltd.). Follow the instructions to add samples, incubate, wash, and perform the colorimetric reaction. The absorbance values ​​of each group of samples were read at a wavelength of 450 nm, and the concentrations of inflammatory factors were calculated. Each sample was set up with 3 replicate wells, and the average value was taken as the result.

[0064] (2) Analysis of experimental results Experimental results are as follows Figure 4 As shown, the specific manifestations are as follows: In the negative control group, the serum levels of IL-6, TNF-α, and IL-1β were significantly elevated, suggesting that strenuous exercise induced a significant inflammatory response. In the positive control group, all inflammatory factors decreased to varying degrees, which is consistent with the expected effect of the known anti-inflammatory component oyster peptide. In the experimental group, the levels of the three inflammatory factors all showed a decreasing trend with increasing dosage of the composition: IL-6: The high-dose group was significantly lower than the negative group, and close to the positive control level; TNF-α: The decrease was most significant in the high-dose group, followed by the medium-dose group; IL-1β: The high-dose group showed a significant decrease, indicating a good dose-dependent effect.

[0065] (3) Summary of results The results of this experiment show that the collagen peptide-saccharide peptide composition of the present invention has good anti-inflammatory effects and can effectively reduce the expression level of key inflammatory factors induced by high-intensity exercise, especially under high-dose conditions.

[0066] The reduction in inflammation levels not only helps alleviate bodily damage and pain but also improves the cellular metabolic environment and accelerates the fatigue repair process. Synergistically with the mechanism of action of enhancing antioxidant capacity, this further validates the systemic regulatory effect of this composition in improving post-exercise fatigue, enhancing its functional positioning in sports nutrition and recovery foods.

[0067] VII. Dosage Optimization and Summary of Functional Mechanisms This invention uses systematic animal experiments to verify the dose-dependent effects of different ratios of collagen peptides and brewer's yeast peptides, and analyzes their mechanism of action in improving post-exercise fatigue from multiple functional index dimensions, establishing a scientifically reasonable correspondence between the proportion of functional components and physiological effects.

[0068] (1) Recommended usage ratio Based on the comparison of experimental data from the low, medium, and high dose groups in this embodiment, it was found that: The medium-dose group (collagen peptide: brewer's yeast peptide = 14:1) and the high-dose group (3:1) were superior to the low-dose group (28:1) in most key functional indicators. The high-dose group showed the most significant effects in lactate clearance, glycogen storage, antioxidant and anti-inflammatory properties. The medium-dose group showed a good balance in urea nitrogen regulation and overall endurance improvement.

[0069] Therefore, taking into account factors such as functional effects, ingredient costs and formulation stability, the recommended optimal ratio is 3–6:1 in mass of collagen peptides to brewer's yeast peptides. Within this range, multiple physiological functions can be synergistically enhanced, and it has high potential for application and promotion.

[0070] (2) Summary of mechanism of action The composition of this invention can synergistically improve post-exercise fatigue and enhance post-exercise recovery efficiency through the following four core mechanisms: 1. Clear metabolic waste products It lowers blood levels of lactic acid (LA) and blood urea nitrogen (BUN), reduces the inhibition of muscle metabolism caused by the accumulation of metabolic waste, and relieves post-exercise soreness and fatigue.

[0071] 2. Increase glycogen reserves It significantly increases glycogen content in the liver and gastrocnemius muscle tissue, providing the body with sufficient energy support, effectively prolonging exercise endurance, and promoting post-exercise energy compensation.

[0072] 3. Enhance antioxidant capacity It enhances the activity of endogenous antioxidant enzyme systems (SOD, GSH-Px), reduces the level of lipid peroxidation product MDA, and alleviates tissue damage caused by oxidative stress.

[0073] 4. Reduce inflammation levels It inhibits the expression of pro-inflammatory factors (IL-6, TNF-α, IL-1β) after exercise, improves the inflammatory environment of muscles, promotes tissue repair, and helps relieve fatigue and recovery.

[0074] In summary, the collagen peptide-brewer's yeast peptide composition proposed in this invention works simultaneously at three levels—energy metabolism, oxidative defense, and inflammation regulation—through multiple targets and a synergistic mechanism, systematically improving exercise-induced fatigue response. It possesses good functional stability and preclinical validation evidence, making it suitable for development as a sports nutrition supplement or a post-exercise functional recovery food.

[0075] As can be seen from the above description of the specific embodiments, the collagen peptide and brewer's yeast peptide composition provided by the present invention has a significant effect on improving post-exercise fatigue. It can work synergistically through multiple mechanisms to effectively remove metabolic waste, increase glycogen reserves, and enhance antioxidant and anti-inflammatory capabilities, thereby improving post-exercise recovery efficiency. Animal experimental results have verified its good bioactivity and safety, showing clear application prospects and suitability for the development of functional foods or sports nutrition products.

[0076] It should be noted that the embodiments disclosed in this invention are only for the purpose of helping to understand the core technical concept of this invention. For those skilled in the art, all improvements, substitutions, equivalent modifications, and additional functional adjustments made without departing from the original technical concept of this invention should fall within the protection scope defined by the claims of this invention.

Claims

1. A composition for improving post-exercise fatigue, characterized in that, The composition comprises collagen peptides and brewer's yeast peptides, wherein the mass ratio of collagen peptides to brewer's yeast peptides is 3-14:

1.

2. The composition according to claim 1, characterized in that, The mass ratio of collagen peptides to brewer's yeast peptides is 3-6:

1.

3. The composition according to claim 2, characterized in that, The mass ratio of collagen peptides to brewer's yeast peptides is 3:

1.

4. The composition according to claim 3, characterized in that, The brewer's yeast peptide contains peptide segments with amino acid sequences of LGGPLL and LGPTGISM, and the content of peptide segments with a molecular weight of less than 1000 Da accounts for more than 50% of the total mass of the brewer's yeast peptide.

5. The composition according to claim 4, characterized in that, It also contains one or more additional active peptides selected from casein peptides, elastin peptides, oyster peptides, and sea cucumber peptides.

6. The composition according to claim 5, characterized in that, It also contains one or more active proteins selected from non-denatured type II collagen, whey protein concentrate, and instant soy flour.

7. The composition according to claim 6, characterized in that, It also contains one or more components selected from polydextrose, theanine, and olive fruit powder.

8. The composition according to claim 7, characterized in that, The composition comprises, by weight, 45-50 parts collagen peptides, 3.5-14 parts brewer's yeast peptides, and a total of 35-50 parts other components, including excipients and / or flavoring agents and / or functional ingredients.

9. A sports nutrition food, characterized in that, The composition comprising the post-exercise fatigue-reducing composition of claim 8.

10. The use of the composition of claim 1 in the preparation of products for improving post-exercise fatigue, and / or enhancing exercise endurance, and / or promoting post-exercise recovery.

Images