A whitening, anti-aging composition containing fibronectin and its preparation method and application

Abstract

The application discloses a whitening and anti-aging composition containing fibronectin and a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition comprises, in mass parts, an active component: recombinant humanized fibronectin and a fermented plant polysaccharide compound, wherein the fermented plant polysaccharide compound is composed of plant polysaccharides prepared through microbial fermentation; and further comprising an auxiliary component acceptable in the field of cosmetics. The application realizes synergistic effects of whitening, anti-aging and moisturizing through scientific compounding of the recombinant humanized fibronectin and the specific fermented plant polysaccharide, and simultaneously solves the core problems of easy inactivation of fibronectin, poor transdermal absorption of plant polysaccharide and insufficient stability of a compounded system in the prior art. The prepared composition has good transdermal absorption effect, excellent storage stability and light stability, the preparation method is simple and controllable, is suitable for industrial large-scale production, and can be widely applied to various whitening and anti-aging cosmetics.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a whitening and anti-aging composition containing fibronectin, its preparation method, and its application. Background Technology

[0002] As consumers' demands for cosmetic efficacy continue to upgrade, whitening and anti-aging have become core demand areas in the cosmetics industry, making bioactive ingredients that combine safety and high efficacy a hot research topic. Fibronectin is a high-molecular-weight glycoprotein widely found in human tissues and body fluids, playing a core role in regulating cell adhesion, proliferation, migration, and differentiation. Recombinant humanized fibronectin, prepared through genetic engineering, is highly homologous to human FN sequences and has no immunogenicity, and has been widely used in the field of skin repair. Existing research shows that fibronectin can help improve signs of aging such as fine lines and sagging skin by promoting fibroblast proliferation and extracellular matrix synthesis. However, its application alone has significant limitations: on the one hand, recombinant humanized fibronectin is easily affected by factors such as temperature, light, and pH in aqueous cosmetic systems, leading to conformational changes, rapid loss of bioactivity, and poor storage and light stability; on the other hand, the whitening effect of fibronectin alone is weak, failing to meet consumers' comprehensive whitening needs, and its anti-aging effect is singular, making it difficult to achieve multi-pathway synergistic anti-aging through anti-oxidation, collagen synthesis, and barrier repair.

[0003] Plant polysaccharides are a class of natural high-molecular-weight compounds derived from plants. They possess various biological activities such as moisturizing, anti-oxidation, and immunomodulation. Due to their high safety and wide availability, they have become commonly used functional ingredients in cosmetics. However, polysaccharides obtained through conventional plant extraction face significant technical bottlenecks: First, natural plant polysaccharides are mostly high-molecular-weight polymers, with weight-average molecular weights generally exceeding 100 kDa, and even reaching millions of Da. They are difficult to penetrate the stratum corneum of the skin and can only remain on the skin surface to exert a moisturizing effect, failing to fully realize their deep anti-aging and whitening effects. Second, high-molecular-weight plant polysaccharides are prone to hydrogen bonding or electrostatic interactions with protein-based active ingredients in cosmetic systems, leading to phenomena such as flocculation, stratification, turbidity, and precipitation. This severely affects the product's appearance stability and shelf life, and can also cause the encapsulation and inactivation of protein components, further reducing efficacy. Third, plant polysaccharides obtained through conventional water extraction and alcohol precipitation methods have low purity, high impurity content, and poor batch stability, easily leading to problems such as product discoloration and increased risk of allergies.

[0004] Therefore, developing a composition containing fibronectin that has excellent whitening, anti-aging, and moisturizing effects, as well as excellent storage stability, light stability, good transdermal absorption, and good compatibility with cosmetic systems has become a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0005] The purpose of this invention is to overcome the above-mentioned defects of the prior art and provide a whitening and anti-aging composition containing fibronectin, its preparation method and application. This composition achieves synergistic effects of whitening, anti-aging and moisturizing by scientifically combining recombinant humanized fibronectin with specific fermented plant polysaccharides. At the same time, it solves the core problems of easy inactivation of fibronectin, poor transdermal absorption of plant polysaccharides and insufficient system stability in the prior art. The preparation method is simple and controllable and suitable for large-scale industrial production.

[0006] The objective of this invention can be achieved through the following technical solutions: A whitening and anti-aging composition containing fibronectin, comprising, by weight, active components: 0.001-5 parts of recombinant humanized fibronectin and 1-20 parts of fermented plant polysaccharide complex; wherein the fermented plant polysaccharide complex is composed of 2-3 kinds of plant polysaccharides obtained by microbial fermentation.

[0007] Further, the recombinant human fibronectin is 0.01-2 parts by weight.

[0008] Furthermore, the fermented plant polysaccharide complex is selected from 2-3 kinds of fermented Tremella fuciformis polysaccharide, fermented Dendrobium officinale polysaccharide, and fermented Poria cocos polysaccharide.

[0009] Further, by weight, the fermented plant polysaccharide complex includes 1-10 parts of fermented Tremella fuciformis polysaccharide, 1-10 parts of fermented Dendrobium officinale polysaccharide, and 0-10 parts of fermented Poria cocos polysaccharide.

[0010] Further, by weight, the composition also includes excipient components: 5-30 parts of humectant, 0.01-0.2 parts of chelating agent, 0.05-0.5 parts of pH adjuster, 0.1-1 parts of preservative, and deionized water to make up to 100 parts.

[0011] Further, the humectant is selected from one or more of glycerin, 1,3-propanediol, glycerol, sorbitol, polyethylene glycol-400, and betaine; the chelating agent is selected from one or more of disodium EDTA, tetrasodium EDTA, and sodium phytate; the pH adjuster is selected from one or more of sodium citrate, citric acid, disodium hydrogen phosphate, and sodium dihydrogen phosphate; and the preservative is selected from one or more of p-hydroxyacetophenone, 1,2-hexanediol, capryloyl hydroxamic acid, and phenoxyethanol.

[0012] This invention also provides a method for preparing the above-mentioned whitening and anti-aging composition containing fibronectin, comprising the following steps: S1: Weigh out the water-soluble raw materials and deionized water from the excipient components according to the mass fraction, add them to the aqueous phase pot, mix and heat to 75-80℃, keep warm and stir for 20-30 minutes at a stirring speed of 20-40 rpm, until all components are completely dissolved to obtain an aqueous phase mixture; S2: Cool the aqueous phase mixture to 40-45℃, add the preservative, and homogenize and stir for 3-5 minutes at a speed of 2000-3000 rpm until the system is uniform and transparent and there are no visible insoluble substances. S3: Continue cooling to 25-30℃, add recombinant human fibronectin and fermented plant polysaccharide complex according to the mass fraction, stir at low speed for 15-25 minutes, stirring speed 15-30 rpm, until completely dissolved and the system is uniform, to obtain a mixed system; S4: The mixture is filtered and sterilized using a 0.22μm microporous membrane to obtain the whitening and anti-aging composition containing fibronectin.

[0013] This invention also provides a method for preparing the above-mentioned fermented plant polysaccharide, comprising the following steps: X1: Raw material pretreatment: Take the corresponding dried plant raw material, crush it through an 80-120 mesh sieve to obtain plant powder, add deionized water at a material-to-liquid ratio of 1g:10-30mL, soak at room temperature for 1-3 hours, autoclave at 121℃ for 20-30 minutes, cool to room temperature to obtain plant culture medium; X2: Seed culture preparation: Inoculate the starting strain into the corresponding liquid culture medium and incubate at 30-37℃ for 18-24 hours to obtain the seed culture. The viable cell concentration of the seed culture should be ≥1×10⁻⁶. 9 CFU / mL; X3: Fermentation culture: Inoculate the seed liquid into the plant culture medium at an inoculation rate of 2%-5% (v / v), and ferment at a constant temperature of 30-37℃ with stirring for 48-72 hours at a stirring speed of 100-200 rpm to obtain the fermentation broth; X4: Inactivation and impurity removal: Boil the fermentation broth at 100℃ for 10-15 min to inactivate it, cool it to room temperature, centrifuge at 4000-6000 rpm for 15-20 min, take the supernatant, filter it with a 0.22μm microfiltration membrane to remove impurities, and obtain a clear filtrate. X5: Refining and Drying: The clarified filtrate was concentrated by ultrafiltration using an ultrafiltration membrane with a molecular weight cutoff of 10 kDa. The concentrate was collected, and 3-5 times the volume of anhydrous ethanol was added to the concentrate. The mixture was allowed to stand at 4°C for 12-24 hours to precipitate. The precipitate was collected by centrifugation, washed 2-3 times with anhydrous ethanol, and then freeze-dried under vacuum to obtain the corresponding fermented plant polysaccharide.

[0014] Furthermore, the starting strain for the fermented Tremella fuciformis polysaccharide is Lactobacillus plantarum, the starting strain for the fermented Dendrobium officinale polysaccharide is Lactobacillus acidophilus, and the starting strain for the fermented Poria cocos polysaccharide is Bifidobacterium.

[0015] The present invention also provides the application of the above-mentioned whitening and anti-aging composition containing fibronectin in the preparation of cosmetics with whitening, anti-aging and moisturizing effects.

[0016] Furthermore, the dosage form of the cosmetic is any one of serum, lotion, face cream, toner, facial mask liquid, and eye cream.

[0017] Furthermore, in the cosmetic, the whitening and anti-aging composition containing fibronectin accounts for 0.5%-20% by mass.

[0018] The beneficial effects of this invention are: (1) This invention achieves multi-pathway synergistic effects of whitening, anti-aging, and moisturizing, demonstrating outstanding inventiveness. Through the scientific combination of recombinant human fibronectin and 2-3 specific fermented plant polysaccharides, this invention constructs a comprehensive efficacy system encompassing "deep repair + anti-oxidation + collagen synthesis + melanin inhibition + long-lasting moisturizing." Specifically, recombinant human fibronectin can activate cell signaling pathways, promote fibroblast proliferation and type I and type III collagen synthesis, repair the skin barrier, and delay skin aging. Fermented plant polysaccharides can scavenge free radicals, inhibit tyrosinase activity, and block melanin transport, exerting antioxidant and whitening effects. Simultaneously, their small molecular structure allows for rapid penetration into the dermis, synergistically enhancing cell proliferation and collagen synthesis efficiency in conjunction with fibronectin. Experimental verification shows that the anti-aging, whitening, and moisturizing effects of the composition of this invention are superior to those of using recombinant human fibronectin or fermented plant polysaccharides alone, and also superior to the combination system of unfermented polysaccharides and fibronectin, achieving a synergistic effect of 1+1>2, rather than a simple additive effect.

[0019] (2) Significantly improved transdermal absorption efficiency of active components, solving the technical bottleneck of the inability of traditional plant polysaccharides to fully exert their efficacy. This invention uses specific microbial fermentation technology to modify plant polysaccharides. Through various glycosidases produced by microorganisms, the plant polysaccharides are directionally degraded. The weight-average molecular weight of the fermented plant polysaccharides obtained is concentrated between 2kDa and 10kDa, with uniform molecular weight distribution and a polydispersity index ≤1.2, which is lower than that of unfermented polysaccharides obtained by conventional water extraction and alcohol precipitation. Transdermal experiments using the Franz diffusion cell verified that the 24-hour cumulative transdermal permeation rate of the fermented plant polysaccharides of this invention can reach more than 35%. At the same time, the small molecule fermented polysaccharides can act as a penetration enhancer, significantly improving the skin retention and transdermal absorption rate of recombinant humanized fibronectin, allowing the bioactivity of fibronectin to be fully exerted in the deep layers of the skin, fundamentally solving the problems of poor transdermal absorption and limited efficacy of traditional polysaccharides.

[0020] (3) The storage and light stability of the composition are significantly improved, solving the industry pain points of easy inactivation of fibronectin and easy stratification and flocculation of protein-polysaccharide complex systems. On the one hand, the low molecular weight polysaccharide obtained by fermentation in this invention has a uniform molecular weight distribution and uniform surface charge, and has good compatibility with recombinant humanized fibronectin. It will not cause stratification, turbidity, or precipitation caused by electrostatic flocculation or hydrogen bonding. The system remains uniform throughout the long-term storage process. On the other hand, fermented plant polysaccharides can form a dense molecular network structure in the composition system, which can encapsulate recombinant humanized fibronectin and prevent it from undergoing conformational changes due to factors such as temperature, light, and pH, thus significantly improving the activity retention rate of fibronectin.

[0021] (4) The preparation method is simple and controllable, with high safety, and is suitable for large-scale industrial production. The fermentation of plant polysaccharides in this invention uses food-grade microbial strains. The fermentation process is green and environmentally friendly, with no toxic or harmful reagent residues. The resulting polysaccharides have high purity and low allergenicity, meeting the safety requirements for cosmetic raw materials. The preparation process of the composition adopts a low-temperature addition of active components process, which maximizes the preservation of the bioactivity of recombinant human fibronectin and fermented plant polysaccharides. The process steps are simple, the parameters are controllable, the batch stability is good, no special equipment is required, and it can be directly adapted to existing cosmetic industrial production lines, possessing extremely high industrial application value. Detailed Implementation

[0022] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0023] Preparation of fermented plant polysaccharides The following preparation examples are used to prepare the fermented plant polysaccharides used in this invention, and the control preparation examples are used to prepare the control polysaccharides used in the comparative examples.

[0024] Preparation Example 1: Preparation of Fermented Tremella Polysaccharide Step 1: Raw material pretreatment: Take dried tremella fruiting bodies, crush them with a universal pulverizer, pass them through a 100-mesh sieve to obtain tremella powder; weigh 100g of tremella powder, add 2000mL of deionized water, with a material-to-liquid ratio of 1g:20mL, soak at room temperature for 2 hours, autoclave at 121℃ for 25 minutes, and cool naturally to room temperature to obtain tremella culture medium. Step 2: Seed culture preparation: *Lactobacillus plantarum* (MICROBIOLOGICS® 01144P) was inoculated into MRS liquid medium and cultured at 37℃ for 20 h to obtain the seed culture. The viable cell concentration of the seed culture was determined to be 2.5 × 10⁻⁶ using the plate count method. 9 CFU / mL; Step 3: Fermentation culture: Inoculate the seed liquid into the tremella culture medium at an inoculation rate of 3% (v / v), and ferment at a constant temperature of 35℃ with stirring for 60 hours at a stirring speed of 150 rpm. After the fermentation is completed, the fermentation liquid is obtained. Step 4: Inactivation and impurity removal: Boil the fermentation broth at 100℃ for 12 minutes to inactivate it, cool it naturally to room temperature, centrifuge at 5000 rpm for 18 minutes, take the supernatant, filter it with a 0.22 μm polyethersulfone microfiltration membrane to remove impurities, and obtain a clear filtrate; Step 5: Purification and Drying: The clarified filtrate was concentrated by ultrafiltration using a spiral-wound ultrafiltration membrane with a molecular weight cutoff of 10 kDa, reducing it to 1 / 5 of its original volume. The concentrate was collected. Four times the volume of anhydrous ethanol was added to the concentrate, and the mixture was stirred until homogeneous. The mixture was then allowed to stand at 4°C for 18 hours to precipitate. The precipitate was collected by centrifugation at 5000 rpm for 15 minutes. The precipitate was washed three times with anhydrous ethanol and then dried in a vacuum freeze dryer for 48 hours to obtain fermented Tremella fuciformis polysaccharide. Gel permeation chromatography (GPC) analysis showed that the weight-average molecular weight of the fermented Tremella fuciformis polysaccharide was 5.6 kDa, and the polydispersity index was 1.12.

[0025] Preparation Example 2: Preparation of Fermented Dendrobium officinale Polysaccharide Step 1: Raw material pretreatment: Take dried Dendrobium officinale stem segments, crush them through a 100-mesh sieve to obtain Dendrobium officinale powder; weigh 100g of Dendrobium officinale powder, add 2500mL of deionized water, with a material-to-liquid ratio of 1g:25mL, soak at room temperature for 3h, autoclave at 121℃ for 30min, and cool naturally to room temperature to obtain Dendrobium officinale culture medium; Step 2: Seed culture preparation: Lactobacillus acidophilus (CICC 6075) was inoculated into MRS liquid medium and cultured at 37℃ for 22 h to obtain the seed culture. The viable cell concentration of the seed culture was determined to be 1.8 × 10⁻⁶ using the plate count method. 9 CFU / mL; Step 3: Fermentation culture: Inoculate the seed liquid into the Dendrobium officinale culture medium at an inoculation amount of 4% (v / v), and ferment at a constant temperature of 37℃ with stirring for 72 hours at a stirring speed of 120 rpm. After the fermentation is completed, the fermentation broth is obtained. Step 4: Inactivation and impurity removal: Boil the fermentation broth at 100℃ for 15 minutes to inactivate it, cool it naturally to room temperature, centrifuge at 6000 rpm for 15 minutes, take the supernatant, filter it with a 0.22 μm polyethersulfone microfiltration membrane to remove impurities, and obtain a clear filtrate; Step 5: Refining and Drying: The clarified filtrate was concentrated by ultrafiltration using a spiral-wound ultrafiltration membrane with a molecular weight cutoff of 10 kDa, reducing it to 1 / 6 of its original volume. The concentrate was collected. Five volumes of anhydrous ethanol were added to the concentrate, and the mixture was stirred until homogeneous. The mixture was then allowed to stand at 4°C for 24 hours to precipitate. The precipitate was collected by centrifugation at 6000 rpm for 12 minutes. The precipitate was washed twice with anhydrous ethanol and then dried in a vacuum freeze dryer for 48 hours to obtain fermented Dendrobium officinale polysaccharide. GPC analysis showed that the weight-average molecular weight of the fermented Dendrobium officinale polysaccharide was 4.2 kDa, and the polydispersity index was 1.08.

[0026] Preparation Example 3: Preparation of Fermented Poria Cocos Polysaccharide Step 1: Raw material pretreatment: Take dried Poria cocos sclerotia, crush them through a 120-mesh sieve to obtain Poria cocos powder; weigh 100g of Poria cocos powder, add 1500mL of deionized water, with a material-to-liquid ratio of 1g:15mL, soak at room temperature for 1.5h, autoclave at 121℃ for 20min, and cool naturally to room temperature to obtain Poria cocos culture medium; Step 2: Seed culture preparation: Bifidobacterium (CICC 6071) was inoculated into MRS liquid medium and anaerobically cultured at 37℃ for 24 h to obtain the seed culture. The viable cell concentration of the seed culture was determined to be 1.2 × 10⁻⁶ using the plate count method. 9 CFU / mL; Step 3: Fermentation culture: Inoculate the seed liquid into the Poria cocos culture medium at an inoculation rate of 2.5% (v / v), and carry out anaerobic constant temperature fermentation at 36℃ with stirring at a stirring speed of 180 rpm for 56 hours. After the fermentation is completed, the fermentation broth is obtained. Step 4: Inactivation and impurity removal: Boil the fermentation broth at 100℃ for 10 min to inactivate it, cool it naturally to room temperature, centrifuge at 4500 rpm for 20 min, take the supernatant, filter it with a 0.22 μm polyethersulfone microfiltration membrane to remove impurities, and obtain a clear filtrate; Step 5: Refining and Drying: The clarified filtrate was concentrated by ultrafiltration using a spiral-wound ultrafiltration membrane with a molecular weight cutoff of 10 kDa, reducing it to 1 / 4 of its original volume. The concentrate was collected. Three times the volume of anhydrous ethanol was added to the concentrate, and the mixture was stirred until homogeneous. The mixture was then allowed to stand at 4°C for 12 hours to precipitate. The precipitate was collected by centrifugation at 4500 rpm for 20 minutes. The precipitate was washed three times with anhydrous ethanol and then dried in a vacuum freeze dryer for 48 hours to obtain fermented Poria cocos polysaccharide. GPC analysis showed that the weight-average molecular weight of the fermented Poria cocos polysaccharide was 6.8 kDa, and the polydispersity index was 1.15.

[0027] Preparation of unfermented Tremella polysaccharide (Comparative Preparation Example 1) The polysaccharide was prepared using a conventional water extraction and alcohol precipitation method: Dried Tremella fruiting bodies were pulverized and passed through a 100-mesh sieve. 100g of Tremella powder was weighed and added to 2000mL of deionized water. The mixture was extracted three times at 95℃ for 2 hours each time. The extracts were combined, centrifuged at 5000rpm for 15 minutes, and the supernatant was collected and concentrated to 1 / 5 of its original volume. Four times the volume of anhydrous ethanol was added, and the mixture was allowed to stand at 4℃ for 18 hours to precipitate. The precipitate was collected by centrifugation, washed three times with anhydrous ethanol, and then freeze-dried under vacuum to obtain unfermented Tremella polysaccharide. GPC analysis showed that the weight-average molecular weight of this unfermented Tremella polysaccharide was 862kDa, and the polydispersity index was 2.35.

[0028] Preparation of unfermented Dendrobium officinale polysaccharide (Comparative Preparation Example 2) The polysaccharide was prepared using a conventional water extraction and alcohol precipitation method: dried Dendrobium officinale stem segments were pulverized and passed through a 100-mesh sieve. 100g of Dendrobium officinale powder was weighed and added to 2500mL of deionized water. The mixture was extracted three times at 90℃ for 2.5h each time. The extracts were combined, centrifuged at 6000rpm for 12min, and the supernatant was collected and concentrated to 1 / 6 of its original volume. Five times the volume of anhydrous ethanol was added, and the mixture was allowed to stand at 4℃ for 24h to precipitate. The precipitate was collected by centrifugation, washed twice with anhydrous ethanol, and then freeze-dried under vacuum to obtain unfermented Dendrobium officinale polysaccharide. GPC analysis showed that the weight-average molecular weight of this unfermented Dendrobium officinale polysaccharide was 627kDa, and the polydispersity index was 2.18.

[0029] Preparation of Tremella polysaccharide by enzymatic hydrolysis (Comparative Preparation Example 3) Unfermented Tremella fuciformis polysaccharide was prepared into a 10 mg / mL aqueous solution. Cellulase and pectinase (mass ratio 1:1) were added at 2% of the substrate mass. Enzymatic hydrolysis was carried out at 50℃ and pH 4.5 for 4 h. The enzymes were then inactivated by boiling at 100℃ for 10 min. After cooling, the supernatant was collected by centrifugation, concentrated by ultrafiltration, precipitated with alcohol, and freeze-dried to obtain the enzymatically hydrolyzed Tremella fuciformis polysaccharide. GPC analysis showed that the weight-average molecular weight of the enzymatically hydrolyzed Tremella fuciformis polysaccharide was 12.6 kDa and the polydispersity index was 1.87.

[0030] Examples of whitening and anti-aging compositions containing fibronectin In the following examples, all components are expressed in parts by weight. The fermented plant polysaccharides used are all products obtained from the preparation examples 1-3 above. The recombinant human fibronectin is purchased from commercially available cosmetic-grade raw materials with a purity of ≥95%. The excipients are all conventional cosmetic-grade raw materials.

[0031] Example 1 This embodiment provides a whitening and anti-aging composition containing fibronectin, with the following formula by weight: Recombinant human fibronectin (Guangdong Saiyuan Biomedical Technology Co., Ltd.) 0.1 parts, fermented Tremella fuciformis polysaccharide 8 parts, fermented Dendrobium officinale polysaccharide 7 parts, glycerol 12 parts, 1,3-propanediol 8 parts, disodium EDTA 0.05 parts, sodium citrate 0.2 parts, p-hydroxyacetophenone 0.3 parts, 1,2-hexanediol 0.5 parts, deionized water 63.85 parts.

[0032] Preparation method: Step 1: Weigh out deionized water, glycerol, 1,3-propanediol, disodium EDTA, and sodium citrate according to the mass ratio, add them to the aqueous phase pot, mix and heat to 78°C, keep warm and stir for 25 minutes at a stirring speed of 30 rpm until all components are completely dissolved to obtain an aqueous phase mixture. Step 2: Cool the aqueous mixture to 42°C, add p-hydroxyacetophenone and 1,2-hexanediol, and homogenize and stir for 4 minutes at a speed of 2500 rpm until the system is uniform and transparent and there are no visible insoluble substances. Step 3: Continue cooling to 28℃, add recombinant human fibronectin, fermented tremella polysaccharide, and fermented dendrobium officinale polysaccharide according to the mass fraction, stir at low speed for 20 minutes at 25 rpm until completely dissolved and the system is uniform, to obtain a mixed system; Step 4: Filter the mixture through a 0.22μm microporous membrane to sterilize it, and obtain the whitening and anti-aging composition containing fibronectin.

[0033] Example 2 This embodiment provides a whitening and anti-aging composition containing fibronectin, with the following formula by weight: 0.5 parts recombinant human fibronectin, 6 parts fermented Tremella fuciformis polysaccharide, 5 parts fermented Dendrobium officinale polysaccharide, 4 parts fermented Poria cocos polysaccharide, 15 parts glycerol, 10 parts 1,3-propanediol, 0.08 parts disodium EDTA, 0.1 parts citric acid, 0.15 parts sodium citrate, 0.4 parts p-hydroxyacetophenone, 0.6 parts 1,2-hexanediol, and 58.17 parts deionized water.

[0034] Preparation method: Step 1: Weigh out deionized water, glycerol, 1,3-propanediol, disodium EDTA, citric acid, and sodium citrate according to the mass ratio, add them to the aqueous phase pot, mix and heat to 80°C, keep warm and stir for 20 minutes at a stirring speed of 35 rpm until all components are completely dissolved to obtain an aqueous phase mixture. Step 2: Cool the aqueous mixture to 45°C, add p-hydroxyacetophenone and 1,2-hexanediol, and homogenize and stir for 5 minutes at a speed of 3000 rpm until the system is uniform and transparent and there are no visible insoluble substances. Step 3: Continue cooling to 30℃, add recombinant human fibronectin, fermented tremella polysaccharide, fermented dendrobium officinale polysaccharide and fermented poria cocos polysaccharide according to the mass fraction, stir at low speed for 25 minutes at 20 rpm until completely dissolved and the system is uniform, to obtain a mixed system; Step 4: Filter the mixture through a 0.22μm microporous membrane to sterilize it, and obtain the whitening and anti-aging composition containing fibronectin.

[0035] Example 3 This embodiment provides a whitening and anti-aging composition containing fibronectin, with the following formula by weight: 2 parts recombinant human fibronectin, 9 parts fermented Tremella fuciformis polysaccharide, 8 parts fermented Poria cocos polysaccharide, 10 parts glycerol, 5 parts sorbitol, 0.1 parts disodium EDTA, 0.2 parts disodium hydrogen phosphate, 0.1 parts sodium dihydrogen phosphate, 0.2 parts capryloyl hydroxamic acid, 0.4 parts 1,2-hexanediol, and 65.0 parts deionized water.

[0036] Preparation method: Step 1: Weigh out deionized water, glycerol, sorbitol, disodium EDTA, disodium hydrogen phosphate, and sodium dihydrogen phosphate according to the mass ratio, add them to the aqueous phase pot, mix and heat to 75°C, keep warm and stir for 30 minutes at a stirring speed of 25 rpm until all components are completely dissolved to obtain an aqueous phase mixture. Step 2: Cool the aqueous mixture to 40°C, add octanoyl hydroxamic acid and 1,2-hexanediol, and homogenize and stir for 3 minutes at a speed of 2000 rpm until the system is uniform and transparent and there are no visible insoluble substances. Step 3: Continue cooling to 25℃, add recombinant human fibronectin, fermented tremella polysaccharide and fermented poria polysaccharide according to the mass fraction, stir at low speed for 15 minutes at 30 rpm until completely dissolved and the system is uniform, to obtain a mixed system; Step 4: Filter the mixture through a 0.22μm microporous membrane to sterilize it, and obtain the whitening and anti-aging composition containing fibronectin.

[0037] Comparative Example 1 This comparative example omits recombinant human fibronectin and makes up the mass fraction with deionized water; the remaining components are exactly the same as in Example 1, and the formulation is as follows: Fermented Tremella polysaccharide 8 parts, fermented Dendrobium officinale polysaccharide 7 parts, glycerol 12 parts, 1,3-propanediol 8 parts, disodium EDTA 0.05 parts, sodium citrate 0.2 parts, p-hydroxyacetophenone 0.3 parts, 1,2-hexanediol 0.5 parts, deionized water 63.95 parts.

[0038] Comparative Example 2 This comparative example omits the fermented plant polysaccharide complex (fermented Tremella fuciformis polysaccharide + fermented Dendrobium officinale polysaccharide), and makes up the mass fraction with deionized water. The remaining components are exactly the same as in Example 1, and the formula is as follows: 0.1 parts recombinant human fibronectin, 12 parts glycerol, 8 parts 1,3-propanediol, 0.05 parts disodium EDTA, 0.2 parts sodium citrate, 0.3 parts p-hydroxyacetophenone, 0.5 parts 1,2-hexanediol, and deionized water to a total of 100 parts.

[0039] Comparative Example 3 In this comparative example, the fermented Tremella fuciformis polysaccharide in Example 1 was replaced with an equal mass of unfermented Tremella fuciformis polysaccharide prepared in Control Preparation Example 1, and the fermented Dendrobium officinale polysaccharide was replaced with an equal mass of unfermented Dendrobium officinale polysaccharide prepared in Control Preparation Example 2. The remaining components were exactly the same as in Example 1, and the formulation is as follows: 0.1 parts recombinant human fibronectin, 8 parts unfermented Tremella fuciformis polysaccharide, 7 parts unfermented Dendrobium officinale polysaccharide, 12 parts glycerol, 8 parts 1,3-propanediol, 0.05 parts disodium EDTA, 0.2 parts sodium citrate, 0.3 parts p-hydroxyacetophenone, 0.5 parts 1,2-hexanediol, and deionized water to make up to 100 parts.

[0040] Comparative Example 4 In this comparative example, the fermented Tremella fuciformis polysaccharide and fermented Dendrobium officinale polysaccharide in Example 1 were replaced with an equal total mass of sodium hyaluronate (Hebei Hongtao Bioengineering Co., Ltd.). The remaining components were exactly the same as in Example 1, and the formula is as follows: 0.1 parts recombinant human fibronectin, 15 parts sodium hyaluronate, 12 parts glycerol, 8 parts 1,3-propanediol, 0.05 parts disodium EDTA, 0.2 parts sodium citrate, 0.3 parts p-hydroxyacetophenone, 0.5 parts 1,2-hexanediol, and deionized water to bring the total to 100 parts.

[0041] Comparative Example 5 In this comparative example, the recombinant humanized fibronectin in Example 1 was replaced with an equal mass of recombinant humanized type I collagen (Wuhan Kemike Biomedical Technology Co., Ltd.), and the remaining components were exactly the same as in Example 1. The formulation is as follows: 0.1 parts recombinant humanized type I collagen, 8 parts fermented Tremella fuciformis polysaccharide, 7 parts fermented Dendrobium officinale polysaccharide, 12 parts glycerol, 8 parts 1,3-propanediol, 0.05 parts disodium EDTA, 0.2 parts sodium citrate, 0.3 parts p-hydroxyacetophenone, 0.5 parts 1,2-hexanediol, and deionized water to make up to 100 parts.

[0042] Comparative Example 6 This comparative example is based on Example 1, with the addition of 8 parts of unfermented Tremella polysaccharide. The corresponding mass parts are reduced by deionized water, and the remaining components are exactly the same as in Example 1. The formula is as follows: 0.1 parts recombinant human fibronectin, 8 parts fermented Tremella fuciformis polysaccharide, 7 parts fermented Dendrobium officinale polysaccharide, 8 parts unfermented Tremella fuciformis polysaccharide, 12 parts glycerol, 8 parts 1,3-propanediol, 0.05 parts disodium EDTA, 0.2 parts sodium citrate, 0.3 parts p-hydroxyacetophenone, 0.5 parts 1,2-hexanediol, and deionized water to bring the total to 100 parts.

[0043] Comparative Example 7 This comparative example removes the fermented Dendrobium officinale polysaccharide from Example 1, retaining only the fermented Tremella fuciformis polysaccharide. The mass fraction is made up with deionized water, and the remaining components are identical to those in Example 1. The formula is as follows: 0.1 parts recombinant human fibronectin, 8 parts fermented Tremella polysaccharide, 12 parts glycerol, 8 parts 1,3-propanediol, 0.05 parts disodium EDTA, 0.2 parts sodium citrate, 0.3 parts p-hydroxyacetophenone, 0.5 parts 1,2-hexanediol, and deionized water to make up to 100 parts.

[0044] Comparative Example 8 In this comparative example, the mass fraction of recombinant human fibronectin in Example 1 was adjusted to 0.0005 parts, and deionized water was used to make up the mass fraction. The remaining components were exactly the same as in Example 1, and the formula is as follows: 0.0005 parts recombinant human fibronectin, 8 parts fermented Tremella fuciformis polysaccharide, 7 parts fermented Dendrobium officinale polysaccharide, 12 parts glycerol, 8 parts 1,3-propanediol, 0.05 parts disodium EDTA, 0.2 parts sodium citrate, 0.3 parts p-hydroxyacetophenone, 0.5 parts 1,2-hexanediol, and deionized water to make up to 100 parts.

[0045] Comparative Example 9 In this comparative example, the total mass fraction of the fermented plant polysaccharide complex in Example 1 was adjusted to 0.5 parts, and deionized water was used to make up the mass fraction. The remaining components were exactly the same as in Example 1, and the formula is as follows: 0.1 parts recombinant human fibronectin, 0.25 parts fermented Tremella fuciformis polysaccharide, 0.25 parts fermented Dendrobium officinale polysaccharide, 12 parts glycerol, 8 parts 1,3-propanediol, 0.05 parts disodium EDTA, 0.2 parts sodium citrate, 0.3 parts p-hydroxyacetophenone, 0.5 parts 1,2-hexanediol, and deionized water to a total of 100 parts.

[0046] Comparative Example 10 In this comparative example, the fermented Tremella polysaccharide in Example 1 was replaced with an equal mass of the enzymatically hydrolyzed Tremella polysaccharide prepared in Control Preparation Example 3. The remaining components were exactly the same as in Example 1, and the formula is as follows: 0.1 parts recombinant human fibronectin, 8 parts enzymatically hydrolyzed Tremella fuciformis polysaccharide, 7 parts fermented Dendrobium officinale polysaccharide, 12 parts glycerol, 8 parts 1,3-propanediol, 0.05 parts disodium EDTA, 0.2 parts sodium citrate, 0.3 parts p-hydroxyacetophenone, 0.5 parts 1,2-hexanediol, and deionized water to make up to 100 parts.

[0047] Comparative Example 11 In this comparative example, the mass ratio of fermented Tremella fuciformis polysaccharide to fermented Dendrobium officinale polysaccharide in Example 1 was adjusted to 15:0, that is, only 15 parts of fermented Tremella fuciformis polysaccharide were retained, and the remaining components were exactly the same as in Example 1. The formula is as follows: 0.1 parts recombinant human fibronectin, 15 parts fermented Tremella polysaccharide, 12 parts glycerol, 8 parts 1,3-propanediol, 0.05 parts disodium EDTA, 0.2 parts sodium citrate, 0.3 parts p-hydroxyacetophenone, 0.5 parts 1,2-hexanediol, and deionized water to make up to 100 parts.

[0048] Performance testing To verify the physicochemical stability, transdermal absorption performance, moisturizing effect, whitening effect, and anti-aging effect of the compositions of the present invention, systematic performance tests were conducted on the samples of the above three examples and 11 comparative examples. All tests were conducted in three parallel experiments, and the results were averaged. The test methods all complied with relevant national cosmetic regulations and industry standards.

[0049] 1. Physicochemical stability testing 1.1 Detection Method (1) High temperature stability: Take 20g of the sample to be tested and place it in a sealed colorless glass bottle. Place it in a constant temperature incubator at 45℃ for 3 months. Observe the appearance of the sample at 1 month, 2 months and 3 months and record whether there is layering, turbidity, precipitation and discoloration. After the 3-month test, use ELISA to detect the activity retention rate of recombinant human fibronectin in the sample. Activity retention rate = activity content after test / initial activity content × 100%.

[0050] (2) Low temperature stability: Take 20g of the sample to be tested and place it in a sealed colorless glass bottle. Place it in a -18℃ refrigerator for 3 months. Take it out at 1 month, 2 months and 3 months respectively, thaw it at room temperature and observe the appearance of the sample. Record whether there is layering, turbidity, precipitation or discoloration.

[0051] (3) Stability of cold and heat cycle: Take 20g of the sample to be tested and place it in a sealed colorless glass bottle. Set the cycle conditions: -18℃ freezing for 24h, 45℃ constant temperature for 24h, which is 1 cycle. A total of 10 cycles are performed. After the cycle, observe the appearance of the sample and record whether there is layering, turbidity, precipitation or discoloration.

[0052] (4) Light stability: Take 20g of the sample to be tested and place it in a sealed colorless glass bottle. Place it in a UV light test chamber and irradiate it continuously for 30 days with a 365nm, 30W UV lamp. The irradiation temperature is 25℃. After the test, observe the appearance of the sample and record whether there is layering, turbidity, precipitation, or discoloration. At the same time, use ELISA to detect the activity retention rate of recombinant human fibronectin in the sample.

[0053] 1.2 Test Results The stability test results are shown in Tables 1 and 2.

[0054] Table 1 Results of Sample Appearance Stability Test Example 1 Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Example 2 Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Example 3 Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Comparative Example 1 Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Comparative Example 2 Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Comparative Example 3 Slight turbidity appeared after 2 months, and a small amount of sediment appeared after 3 months. Slight separation after thawing Slight layering, with sediment Slightly cloudy, yellowish Comparative Example 4 Slight turbidity after 1 month, small amount of sediment after 3 months Slightly cloudy after thawing Slightly cloudy with sediment Slight turbidity, slight discoloration Comparative Example 5 Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Slightly cloudy, no sediment Comparative Example 6 Slight turbidity appeared after 1 month, and a small amount of sediment appeared after 3 months. After thawing, there was slight stratification and a small amount of sediment. Slight stratification, large amount of sediment Slightly cloudy, yellowish Comparative Example 7 Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Comparative Example 8 Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Comparative Example 9 Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Comparative Example 10 Slight turbidity after 2 months, and a small amount of sediment after 3 months. Slightly cloudy after thawing Turbid, with a small amount of sediment Slight turbidity, slight discoloration Comparative Example 11 Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Clarified and transparent, with no abnormalities. Table 2 Results of the activity retention rate of recombinant human fibronectin in the samples Example 1 92.3 90.7 Example 2 94.5 93.1 Example 3 95.8 94.2 Comparative Example 2 12.6 0 Comparative Example 3 35.2 21.8 Comparative Example 4 42.7 30.5 Comparative Example 6 28.9 15.3 Comparative Example 10 58.6 45.2 2. Transdermal absorption rate detection 2.1 Detection Method Transdermal absorption experiments were conducted using the Franz vertical diffusion cell method, referring to the relevant specifications in "Test Methods for Transdermal Absorption of Cosmetics". (1) Preparation of ex vivo skin: SPF grade Kunming mice were euthanized, and the abdominal skin was peeled off. Subcutaneous fat tissue was removed, the skin was rinsed with physiological saline, cut into appropriate sizes, and frozen in a -20℃ freezer. The skin was to be used within 24 hours. Before use, the skin was thawed and the integrity of the skin was checked. Damaged skin samples were discarded.

[0055] (2) Experimental setup: The volume of the receiving cell of the Franz diffusion cell is 7 mL and the effective diffusion area is 1.766 cm². The mouse skin is fixed between the supply cell and the receiving cell, with the stratum corneum facing the supply cell and the dermis facing the receiving cell. phosphate buffer (PBS) with pH 7.4 is added to the receiving cell. The mixture is stirred at a constant temperature of 37°C and a stirring speed of 300 rpm. After removing air bubbles, the mixture is equilibrated for 30 min.

[0056] (3) Sample administration: Add 1g of the sample to be tested to the supply pool, and take 0.5mL of receiving solution from the receiving pool at 2h, 4h, 8h, 12h and 24h after administration, and replenish with an equal volume of fresh PBS buffer at 37℃.

[0057] (4) Detection and calculation: The content of recombinant human fibronectin and polysaccharide in the receiving liquid was detected by high performance liquid chromatography (HPLC), and the cumulative permeation amount and transdermal absorption rate over 24 hours were calculated. Transdermal absorption rate = cumulative permeation amount over 24 hours / total amount administered × 100%.

[0058] 2.2 Test Results The results of the transdermal absorption rate test are shown in Table 3.

[0059] Table 3. Results of transdermal absorption rate of samples after 24 hours (%) Example 1 18.6 38.2 Example 2 20.3 41.5 Example 3 22.7 43.8 Comparative Example 2 5.2 - Comparative Example 3 6.8 7.3 Comparative Example 4 8.5 9.6 Comparative Example 5 - 35.7 Comparative Example 7 12.3 22.5 Comparative Example 10 10.2 24.8 Comparative Example 11 13.5 26.1 3. Moisturizing efficacy test 3.1 Detection Method Referring to the "Guideline for Evaluation of Moisturizing Efficacy of Cosmetics" QB / T4256-2011, the moisturizing efficacy was evaluated using human skin tests. (1) Subject selection: 30 healthy volunteers aged 20-45 years, half male and half female, with no history of skin diseases, no abnormalities on the skin of the test site, and no use of any cosmetics or topical drugs on the test site for 3 days before the test. All subjects signed informed consent forms.

[0060] (2) Test environment: temperature 22±1℃, relative humidity 50±5%. Subjects sit quietly in the test environment for 30 minutes, expose the inner side of the forearm of the test site, keep relaxed, and avoid contact with other items throughout the test.

[0061] (3) Test setup: Mark a 4cm×4cm test area on the inside of the subject's forearm, with each area spaced 2cm apart. Randomly assign test samples and blank controls. Apply 20mg of sample evenly to each sample area, and do not apply any sample to the blank control area.

[0062] (4) Detection and calculation: The skin stratum corneum moisture content of the test area was measured before application (base value) and 2h, 4h and 8h after application. Each area was measured 5 times and the average value was taken. The skin moisture content improvement rate was calculated by the formula: Moisture improvement rate = (detected value - base value) / base value × 100%.

[0063] 3.2 Test Results The results of the moisturizing efficacy test are shown in Table 4.

[0064] Table 4 Results of skin moisture content enhancement rate (%) Example 1 68.5 59.2 42.7 Example 2 75.3 66.8 50.5 Example 3 82.6 74.1 58.3 Comparative Example 1 45.2 32.6 18.5 Comparative Example 2 22.3 10.5 3.2 Comparative Example 3 38.7 25.3 12.6 Comparative Example 4 40.2 28.5 15.3 Comparative Example 5 52.6 40.3 25.7 Comparative Example 7 48.5 36.2 22.3 Comparative Example 10 42.3 30.5 16.8 4. Whitening efficacy test 4.1 Detection Method The whitening efficacy of the samples was comprehensively evaluated using an in vitro tyrosinase inhibition rate assay and a B16 melanoma cell melanin production inhibition rate assay.

[0065] (1) Tyrosinase inhibition rate test (DOPA rate oxidation method): Set up a sample group, a blank control group, and a positive control group. Add PBS buffer (pH 6.8), 100 μL of sample solution, and 50 μL of 0.5 mmol / L L-DOPA solution to a 96-well plate. Incubate at 37°C for 10 min. Add 50 μL of 100 U / mL tyrosinase solution and incubate at 37°C for 5 min. Measure the absorbance at 475 nm using a microplate reader and calculate the tyrosinase inhibition rate using the following formula: Tyrosinase inhibition rate = [1 - (Sample A - Blank A) / (Blank A - Blank A Control)] × 100% (2) Assay on the inhibition rate of melanin production in B16 melanoma cells: B16 melanoma cells in logarithmic growth phase were injected at a rate of 1×10⁻⁶. 5 Cells were seeded at a density of [number] cells / well in 6-well plates and cultured for 24 h. Then, culture medium containing the test sample was added, and a blank control group was set up. Cells were cultured for another 72 h. The culture medium was discarded, and the cells were washed twice with PBS. Cells were lysed with 1 mol / L NaOH solution (containing 10% DMSO), and heated at 100℃ for 10 min to dissolve melanin. The absorbance was measured at 405 nm using a microplate reader, and the melanin production inhibition rate was calculated using the following formula: Melanin production inhibition rate = (1 - Sample A / Blank A) × 100% 4.2 Test Results The results of the whitening efficacy test are shown in Table 5.

[0066] Table 5. Results of whitening efficacy test of samples (%) Example 1 68.2 59.7 Example 2 75.6 67.3 Example 3 82.3 74.5 Comparative Example 1 42.5 35.2 Comparative Example 2 8.3 5.6 Comparative Example 3 25.7 18.6 Comparative Example 4 22.3 15.7 Comparative Example 5 38.6 30.5 Comparative Example 7 35.2 28.3 Comparative Example 10 30.5 24.6 5. Anti-aging efficacy testing 5.1 Detection Method The anti-aging efficacy of the samples was comprehensively evaluated using in vitro free radical scavenging rate assay, human skin fibroblast proliferation assay, and type I collagen synthesis assay.

[0067] (1) DPPH free radical scavenging rate test: Set up a sample group, a blank control group, and a positive control group. Add 2 mL of sample solution and 2 mL of 0.2 mmol / L DPPH ethanol solution to a 96-well plate, and react at room temperature in the dark for 30 min. Measure the absorbance at 517 nm using a microplate reader, and calculate the DPPH free radical scavenging rate using the following formula: DPPH free radical scavenging rate = [1 - (Sample A - Sample A blank) / Sample A blank] × 100% (2) Human skin fibroblast proliferation assay (CCK-8 assay): Human skin fibroblasts in the logarithmic growth phase were divided into 5 × 10 3Cells were seeded at a density of [number] cells / well in 96-well plates and cultured for 24 hours. Then, culture medium containing the test sample was added, and a blank control group was set up. The cells were cultured for another 48 hours. CCK-8 reagent was added, and the plates were incubated at 37°C for 2 hours. The absorbance was measured at 450 nm using a microplate reader, and the cell proliferation rate was calculated using the following formula: Cell proliferation rate = (Sample A / Blank A) × 100% (3) Type I collagen synthesis assay: Human skin fibroblasts in the logarithmic growth phase were divided into 1×10 5 Cells were seeded at a density of [number] cells / well in 6-well plates and cultured for 24 h. Then, culture medium containing the test sample was added, and a blank control group was set up. The cells were cultured for another 72 h. The cell supernatant was collected, and the type I collagen content in the supernatant was detected using a human type I collagen ELISA kit. The collagen synthesis enhancement rate was calculated using the following formula: Collagen synthesis enhancement rate = (sample content - blank content) / blank content × 100% 5.2 Test Results The results of the anti-aging efficacy test are shown in Table 6.

[0068] Table 6 Results of Anti-aging Efficacy Tests on Samples Example 1 72.5 158.3 86.7 Example 2 79.6 172.5 102.3 Example 3 85.3 189.7 121.5 Comparative Example 1 48.2 112.5 35.6 Comparative Example 2 12.3 128.7 42.3 Comparative Example 3 25.6 105.3 22.7 Comparative Example 4 22.5 108.6 25.3 Comparative Example 5 45.3 135.2 58.6 Comparative Example 7 38.7 120.5 42.5 Comparative Example 10 35.2 115.7 38.3 The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A whitening and anti-aging composition containing fibronectin, characterized in that, By weight, it includes the active components: 0.001-5 parts of recombinant human fibronectin and 1-20 parts of fermented plant polysaccharide complex; The fermented plant polysaccharide complex is composed of 2-3 kinds of plant polysaccharides obtained by microbial fermentation.

2. The whitening and anti-aging composition containing fibronectin according to claim 1, characterized in that, The recombinant human fibronectin is 0.01-2 parts by weight.

3. The whitening and anti-aging composition containing fibronectin according to claim 1, characterized in that, The fermented plant polysaccharide complex is selected from 2-3 kinds of fermented Tremella fuciformis polysaccharide, fermented Dendrobium officinale polysaccharide, and fermented Poria cocos polysaccharide.

4. The whitening and anti-aging composition containing fibronectin according to claim 3, characterized in that, The fermented plant polysaccharide complex comprises, by weight, 1-10 parts of fermented Tremella fuciformis polysaccharide, 1-10 parts of fermented Dendrobium officinale polysaccharide, and 0-10 parts of fermented Poria cocos polysaccharide.

5. The whitening and anti-aging composition containing fibronectin according to claim 1, characterized in that, By weight, it also includes excipients: 5-30 parts humectant, 0.01-0.2 parts chelating agent, 0.05-0.5 parts pH adjuster, 0.1-1 parts preservative, and deionized water to make up to 100 parts.

6. The method for preparing the whitening and anti-aging composition containing fibronectin according to claim 1, characterized in that, Includes the following steps: S1: Weigh out the water-soluble raw materials and deionized water from the excipient components according to the mass fraction, mix and heat to 75-80℃, keep warm and stir until completely dissolved to obtain an aqueous phase mixture; S2: Cool the aqueous mixture to 40-45℃, add the preservative, and stir homogenously until the system is uniform; S3: Continue cooling to 25-30℃, add recombinant human fibronectin and fermented plant polysaccharide complex by mass, stir at low speed until completely dissolved, and obtain a mixed system; S4: Filter and sterilize the mixture to obtain the whitening and anti-aging composition containing fibronectin.

7. The whitening and anti-aging composition containing fibronectin according to claim 3, characterized in that, The preparation method of fermented plant polysaccharides Includes the following steps: X1: Raw material pretreatment: Take the corresponding dried plant raw materials, crush and sieve them, add deionized water according to the material-liquid ratio to soak, autoclave and cool to obtain plant culture medium; X2: Seed culture preparation: The starting strain is inoculated into the corresponding liquid culture medium and cultured at a constant temperature until the viable cell concentration reaches the target to obtain the seed culture; X3: Fermentation culture: The seed liquid is inoculated into the plant culture medium and fermented at a constant temperature with stirring to obtain the fermentation broth; X4: Inactivation and impurity removal: Boil the fermentation broth to inactivate it, cool it, centrifuge to collect the supernatant, and microfilter to remove impurities to obtain a clear filtrate; X5: Refining and Drying: The clarified filtrate was concentrated by ultrafiltration, anhydrous ethanol was added and allowed to stand to precipitate, the precipitate was collected by centrifugation and washed, and then freeze-dried under vacuum to obtain the corresponding fermented plant polysaccharide.

8. The use of the whitening and anti-aging composition containing fibronectin as described in claim 1 in the preparation of cosmetics with whitening, anti-aging and moisturizing effects.

9. The application according to claim 8, characterized in that, The cosmetic product can be any one of the following: serum, lotion, face cream, toner, facial mask, or eye cream.

10. The application according to claim 8, characterized in that, In the cosmetic product, the whitening and anti-aging composition containing fibronectin is added at a mass ratio of 0.5%-20%.