A gel composition and uses thereof

Abstract

The present application provides a gel composition and its use. The gel composition comprises: (a) at least one sulfated glycosaminoglycan with a molecular weight of 8-100 kDa; (b) at least one selected from the group consisting of hyaluronic acid or its salt with a molecular weight of 20-1000 kDa, acetylated hyaluronic acid or its salt; and (c) pectin, wherein the mass ratio of a) and (b) is 0.1:1-7:1. The gel composition provided by the present application can effectively deposit on the skin surface to form a protective film during the rinsing process, improve the residence of sulfated glycosaminoglycan and hyaluronic acid on the skin surface, and ensure that sulfated glycosaminoglycan and hyaluronic acid can exert efficacy; the sulfated glycosaminoglycan and hyaluronic acid in the gel composition provided by the present application can produce a synergistic effect in efficacy during the residence on the skin surface; the gel composition provided by the present application can effectively improve the problem of skin dryness after skin washing; and the gel composition provided by the present application can reduce the irritation and / or damage of the skin caused by cleaning products.

Description

Technical Field

[0001] This application belongs to the field of daily chemical products technology, specifically relating to a gel composition and its use. Background Technology

[0002] In daily life, our skin interacts with cleansing products every day. Whether it's facial cleanser, shower gel, or shampoo, their core ingredient is surfactant. During washing, surfactants remove the skin's sebum film, affecting the skin's microecology. Furthermore, surfactant emulsification extracts skin lipids, disrupting their orderly arrangement and impairing barrier function. After washing, residual surfactants can adhere to stratum corneum proteins, causing protein denaturation and swelling, disrupting the skin's own NMF (natural moisturizing factor) production mechanism, leading to dry, flaky skin and reduced stratum corneum hydration. When stratum corneum hydration decreases, the skin's surface hydration level declines, weakening the barrier's integrity and tolerance to external stimuli, resulting in dryness, tightness, and other discomfort.

[0003] To reduce skin irritation and damage during the cleansing process, the traditional approach is to use moisturizing and soothing skincare products or gentler cleansing products after cleansing. However, this still cannot effectively prevent the irritation and damage caused by cleansing products to the skin. Summary of the Invention

[0004] In response to the problems existing in the prior art, the inventors of this application have innovatively integrated skin care into the cleansing process. During the cleansing or rinsing process, it effectively improves the skin discomfort caused by improper cleansing (such as the irritation of the skin by cleansers, water temperature, etc.) and effectively maintains or increases the moisture content of the stratum corneum.

[0005] Therefore, the inventors discovered that adding skin-protective components (such as sulfated glycosaminoglycans, hyaluronic acid or its salts, acetylated hyaluronic acid or its salts) to a rinsing device does not allow them to effectively deposit on the skin surface during rinsing. Even when prepared as a gel, it is difficult for them to effectively deposit on the skin surface and exert their effects. Further research revealed that combining sulfated glycosaminoglycans, hyaluronic acid or its salts (or acetylated hyaluronic acid or its salts) in a certain proportion, and using pectin as a gelling agent, allows the resulting gel composition to effectively deposit on the skin surface during rinsing, forming a protective film, maintaining or increasing the moisture content of the stratum corneum, and improving skin discomfort caused by improper cleansing, thus completing this invention.

[0006] This application provides a gel composition and its use.

[0007] Specifically, this application relates to the following aspects: 1. A gel composition comprising: (a) At least one sulfated glycosaminoglycan with a molecular weight of 8-100 kDa; (b) Select at least one from the group consisting of hyaluronic acid or its salts with a molecular weight of 20-1000 kDa, acetylated hyaluronic acid or its salts; and (c) Pectin, The mass ratio of (a) to (b) is 0.1:1 to 7:1.

[0008] 2. The gel composition according to claim 1, wherein the mass ratio of (a) to (b) is 0.5:1 to 6.5:1; Preferably, the mass ratio of (c) to the sum of the masses of (a) and (b) is 3.3:1-15:1.

[0009] 3. The gel composition according to item 1 or 2, wherein in the gel composition, the content of (c) is greater than 6% and less than 30%.

[0010] 4. The gel composition according to any one of items 1-3, wherein the molecular weight of (a) is 8-70 kDa; Preferably, the molecular weight of (b) is 20-800 kDa.

[0011] 5. The gel composition according to any one of claims 1-3, wherein the sulfated glycosaminoglycan comprises one or more of chondroitin sulfate, dermatan sulfate, keratin sulfate, heparin, and heparan sulfate.

[0012] 6. The gel composition according to any one of items 1-5, wherein the total content of (a) and (b) in the gel composition is 0.5% or more.

[0013] 7. Use of any one of the gel compositions described in items 1-6 in improving the retention of (a) and (b) on the skin surface under aqueous conditions.

[0014] 8. According to the use described in item 7, the gel composition is used to improve skin dryness after washing.

[0015] 9. The gel composition of any one of items 1-6 is used to reduce skin irritation and / or damage caused by cleansing products.

[0016] 10. Use of the gel composition according to any one of items 1-6 in the preparation of daily chemical products.

[0017] Technical effects of this application The gel composition provided in this application can effectively deposit on the skin surface during rinsing to form a protective film, improve the retention of sulfated glycosaminoglycans and hyaluronic acid on the skin surface, and ensure that sulfated glycosaminoglycans and hyaluronic acid can exert their effects. Furthermore, the sulfated glycosaminoglycans and hyaluronic acid in the gel composition provided in this application can produce a synergistic effect in efficacy during their stay on the skin surface; Furthermore, the gel composition provided in this application can effectively improve the problem of dry skin after washing; Furthermore, the gel composition provided in this application can reduce the irritation and / or damage to the skin caused by cleansing products; The gel composition provided in this application can endow rinse-off products with beauty and skin care effects, broaden the application scenarios of rinse-off products, and improve the user experience of consumers during the rinse-off process. Attached Figure Description

[0018] Figure 1 The image shown is a scanning electron microscope image of the pseudo-skin of the control group, with a magnification of 60x.

[0019] Figure 2 The image shown is a scanning electron microscope image of the control group's artificial skin, magnified 60 times.

[0020] Figure 3 The images are scanning electron microscope images of the synthetic skin from experimental group 2. The left image is magnified 70 times, and the right image is magnified 200 times.

[0021] Figure 4 The images are scanning electron microscope images of the synthetic skin from experimental group 3. The left image is magnified 70 times, and the right image is magnified 200 times.

[0022] Figure 5 The images shown are scanning electron microscope images of the synthetic skin from experimental group 5. The left image is magnified 70 times, and the right image is magnified 200 times.

[0023] Figure 6 The images are scanning electron microscope images of the synthetic skin of experimental group 9, with the left image magnified at 70x and the right image magnified at 200x.

[0024] Figure 7 The images are scanning electron microscope images of the synthetic skin of experimental group 18, with the left image magnified at 70x and the right image magnified at 200x.

[0025] Figure 8 The images are scanning electron microscope images of the synthetic skin of experimental group 19, with the left image magnified at 70x and the right image magnified at 200x.

[0026] Figure 9The images are scanning electron microscope images of the synthetic skin of experimental group 20, with the left image magnified at 70x and the right image magnified at 200x.

[0027] Figure 10 This is a bar chart showing the Δ cuticle water content.

[0028] Figure 11 A bar chart of skin TEWL values. Detailed Implementation

[0029] The present application is further illustrated below with reference to embodiments. It should be understood that the embodiments are only used to further illustrate and explain the present application and are not intended to limit the present application.

[0030] Unless otherwise defined, technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art. While similar or identical methods and materials may be applied in experimental or practical applications, materials and methods are described herein. In case of conflict, the definitions included herein shall prevail. Furthermore, materials, methods, and examples are for illustrative purposes only and are not intended to be limiting. The present application is further described below with reference to specific embodiments, but is not intended to limit the scope of the application.

[0031] To address the problems existing in the prior art, this application provides a gel composition comprising (a) at least one sulfated glycosaminoglycan with a molecular weight of 8-100 kDa; (b) at least one selected from the group consisting of hyaluronic acid or its salts, acetylated hyaluronic acid or its salts, with a molecular weight of 20-1000 kDa; and (c) pectin, wherein the mass ratio of (a) and (b) is 0.1:1-7:1, for example, it can be 0.1:1, 0.5:1, 0.6:1, 0.7:1, 0.8:1, 0.9:1, 1:1, 1.5:1, 2:1, 2.5:1, 3, 3.5:1, 4:1, 4.5:1, 5:1, 5.5:1, 6:1, 6.5:1, 7:1, and any value between these values.

[0032] In some implementations, the mass ratio of (a) to (b) is 0.5:1 to 6.5:1.

[0033] In some implementations, the mass ratio of (c) to the sum of the masses of (a) and (b) is 3.3:1 to 15:1, for example, it can be 3.3:1, 3.5:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, and any value between these values.

[0034] In some embodiments, the content of (c) in the gel composition is greater than 6% and less than 30%, for example, it can be 6.1%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, etc.

[0035] In some embodiments, the content of (c) in the gel composition is 7%-30%.

[0036] Glycosaminoglycans (GAGs) are core components of the extracellular matrix (ECM) of the skin, possessing skin-protective functions such as moisturizing, anti-aging, and maintaining the skin barrier. They are long linear polysaccharides composed of repeating disaccharide units. Based on differences in sulfation modification, they can be divided into two categories: (1) Non-sulfated GAGs: represented by hyaluronan (HA), which connect N-acetylglucosamine and glucuronic acid through β-1,3 / 1,4 glycosidic bonds; (2) Sulfated glycosaminoglycans (sGAGs): including chondroitin sulfate (CS), dermatan sulfate (DS), heparin, heparan sulfate (HS), and keratin sulfate (KS).

[0037] In some embodiments, the sulfated glycosaminoglycan includes one or more of chondroitin sulfate, dermatan sulfate, keratin sulfate, heparin, and heparan sulfate.

[0038] Acetylated hyaluronic acid or its salts are obtained by acetylation of hyaluronic acid or its salts, and can be commercially available or prepared at home.

[0039] In some embodiments, the degree of substitution of the acetylated hyaluronic acid or its salt is not particularly limited, for example, it can be 50%-95%, such as 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, and any value between these values.

[0040] There are no particular restrictions on the types of hyaluronic acid salts and acetylated hyaluronic acid salts; they can be alkali metal salts of hyaluronic acid, such as sodium salts, potassium salts, magnesium salts, calcium salts, and zinc salts.

[0041] Pectin can form a three-dimensional network gel structure in aqueous systems, serving as the gel framework for gel compositions. When the pectin concentration is too low, such as below 6%, the gel strength is insufficient, leading to excessive disintegration under the shear force of water flow during rinsing. This results in the active ingredients being encapsulated and lost by gel fragments, significantly reducing the skin deposition rate. Conversely, when the pectin concentration is too high, such as above 30%, the gel strength is too high, and the resistance to water flow shear is too strong, preventing effective spread and release of active ingredients on the skin surface, thus limiting deposition efficiency.

[0042] In some embodiments, the molecular weight of (a) is 8-100 kDa, for example, it can be 8 kDa, 9 kDa, 10 kDa, 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 70 kDa, 80 kDa, 90 kDa, 100 kDa, and any value between these values; or for example, it can be 10-30 kDa, 30-50 kDa, 60-85 kDa, 85-100 kDa.

[0043] In some implementations, the molecular weight of (a) is 8-70 kDa.

[0044] In some embodiments, the molecular weight of (a) is 10-50 kDa.

[0045] In some embodiments, the molecular weight of (b) is 20-1000 kDa, for example, it can be 20 kDa, 30 kDa, 50 kDa, 100 kDa, 150 kDa, 200 kDa, 250 kDa, 300 kDa, 350 kDa, 400 kDa, 450 kDa, 500 kDa, 600 kDa, 700 kDa, 800 kDa, 900 kDa, 1000 kDa, and any value between these values; or for example, it can be 30-50 kDa, 50-100 kDa, 200-400 kDa, 800-1000 kDa.

[0046] In some embodiments, the molecular weight of (b) is 20-800 kDa.

[0047] In some embodiments, the molecular weight of (b) is 30-400 kDa.

[0048] In some embodiments, the total content of (a) and (b) in the gel composition can be 0.5% or more from the perspective of efficacy, for example, it can be 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, 15%, 20%, etc., and preferably less than 10% from the perspective of saving production costs.

[0049] In some embodiments, the total content of (a) and (b) in the gel composition is 0.5%-10%.

[0050] In some embodiments, the gel composition comprises (a) at least one sulfated glycosaminoglycan with a molecular weight of 8-100 kDa; (b) at least one selected from the group consisting of hyaluronic acid or a salt thereof with a molecular weight of 20-1000 kDa, acetylated hyaluronic acid or a salt thereof; and (c) pectin, wherein the mass ratio of (a) to (b) is 0.1:1 to 7:1, and the content of (c) in the gel composition is greater than 6% and less than 30%.

[0051] In some embodiments, the gel composition comprises (a) at least one sulfated glycosaminoglycan with a molecular weight of 8-70 kDa; (b) at least one selected from the group consisting of hyaluronic acid or a salt thereof with a molecular weight of 20-800 kDa, acetylated hyaluronic acid or a salt thereof; and (c) pectin, wherein the mass ratio of (a) to (b) is 0.5:1 to 6.5:1, and the content of (c) in the gel composition is 7%-30%.

[0052] In some embodiments, the gel composition comprises (a) at least one sulfated glycosaminoglycan with a molecular weight of 8-100 kDa; (b) at least one selected from the group consisting of hyaluronic acid or a salt thereof with a molecular weight of 20-1000 kDa, acetylated hyaluronic acid or a salt thereof; and (c) pectin, wherein the mass ratio of (a) to (b) is 0.1:1 to 7:1, wherein the content of (c) in the gel composition is greater than 6% and less than 30%, and the total content of (a) and (b) is greater than 0.5%.

[0053] In some embodiments, the gel composition comprises (a) at least one sulfated glycosaminoglycan with a molecular weight of 8-100 kDa; (b) at least one selected from the group consisting of hyaluronic acid or a salt thereof with a molecular weight of 20-1000 kDa, acetylated hyaluronic acid or a salt thereof; and (c) pectin, wherein the mass ratio of (a) to (b) is 0.1:1 to 7:1, wherein the content of (c) in the gel composition is greater than 6% and less than 30%, and the total content of (a) and (b) is 0.5% to 10%.

[0054] In some embodiments, the gel composition comprises (a) at least one sulfated glycosaminoglycan with a molecular weight of 8-70 kDa; (b) at least one selected from the group consisting of hyaluronic acid or a salt thereof with a molecular weight of 20-800 kDa, acetylated hyaluronic acid or a salt thereof; and (c) pectin, wherein the mass ratio of (a) to (b) is 0.5:1 to 6.5:1, the content of (c) in the gel composition is 7%-30%, and the total content of (a) and (b) is more than 0.5%.

[0055] In some embodiments, the gel composition comprises (a) at least one sulfated glycosaminoglycan with a molecular weight of 8-70 kDa; (b) at least one selected from the group consisting of hyaluronic acid or a salt thereof with a molecular weight of 20-800 kDa, acetylated hyaluronic acid or a salt thereof; and (c) pectin, wherein the mass ratio of (a) to (b) is 0.5:1 to 6.5:1, the content of (c) in the gel composition is 7%-30%, and the total content of (a) and (b) is 0.5%-10%.

[0056] This application also provides the use of the above-described gel composition in improving the retention of (a) and (b) on the skin surface under water flow conditions.

[0057] The gel composition provided in this application can effectively deposit on the skin surface to form a protective film during rinsing, thereby improving the retention of sulfated glycosaminoglycans and hyaluronic acid on the skin surface and ensuring that sulfated glycosaminoglycans and hyaluronic acid can exert their effects.

[0058] In some embodiments, the gel composition is used to improve skin dryness after washing.

[0059] The gel composition provided in this application can also reduce the irritation and / or damage to the skin caused by cleansing products.

[0060] In some implementations, the cleaning product contains a surfactant.

[0061] The use of the gel composition provided in this application in the preparation of daily chemical products. The gel composition can be prepared into daily chemical products for use in rinsing equipment.

[0062] Daily chemical products are fine chemicals that people frequently use in their daily lives, mainly including cosmetics, detergents, personal hygiene products, and household cleaning products.

[0063] The gel composition provided in this application can be used in the following ways: The gel composition is placed in a rinsing device, and when the rinsing device is turned on, the flowing water carries the gel composition to the skin surface.

[0064] Example The raw material information involved in the embodiments and comparative examples of this application is shown in Table 1.

[0065] Table 1

[0066] Experimental Example Preparation of gel composition Experimental group 1 Weigh 10% of pectin and add it to pure water. Heat and stir at 50°C until completely dissolved without any powder lumps. Cool and solidify to obtain gel composition 1.

[0067] Experimental group 2 1. Weigh 2% of CS powder (molecular weight 30-50kDa), add pure water and heat and stir at 50℃ until completely dissolved; 2. Add 10% pectin, heat and stir until completely dissolved without any powder lumps, cool and solidify to obtain gel composition 2.

[0068] Experimental group 3 1. Weigh 2% HA powder (molecular weight 200-400kDa), add pure water and heat and stir at 50℃ until completely dissolved; 2. Add 10% pectin, heat and stir until completely dissolved without any powder lumps, cool and solidify to obtain gel composition 3.

[0069] Experimental group 4 1. Weigh out CS (molecular weight 30-50kDa) and HA powder (molecular weight 200-400kDa) separately, with a mass ratio of 1:1. The total amount of CS and HA added is 2%. Add pure water and heat and stir at 50°C until completely dissolved. 2. Add 10% pectin, heat and stir until completely dissolved without any powder lumps, cool and solidify to obtain gel composition 4.

[0070] Experimental group 5 The preparation method is the same as that of experimental group 4, except that the mass ratio of CS to HA is 2:1, resulting in gel composition 5.

[0071] Experimental group 6 The preparation method is the same as that of experimental group 4, except that the mass ratio of CS to HA is 4:1, resulting in gel composition 6.

[0072] Experimental group 7 The preparation method was the same as that of experimental group 4, except that the mass ratio of CS to HA was 6:1, resulting in gel composition 7.

[0073] Experimental group 8 The preparation method is the same as that of experimental group 4, except that the mass ratio of CS to HA is 8:1, resulting in gel composition 8.

[0074] Experimental group 9 Weigh out CS (molecular weight 30-50kDa) and HA powder (molecular weight 200-400kDa) respectively, with a mass ratio of 2:1. The total amount of CS and HA added is 2%. Add pure water and heat and stir at 50°C until completely dissolved. Cool and solidify to obtain gel composition 9.

[0075] Experimental group 10 Weigh out CS (molecular weight 30-50kDa) and HA powder (molecular weight 200-400kDa) respectively, with a mass ratio of 2:1. The total amount of CS and HA added is 4%. Add pure water and heat and stir at 50°C until completely dissolved. Cool and solidify to obtain gel composition 10.

[0076] Experimental group 11 The preparation method was the same as that of experimental group 5, except that the total amount of CS and HA added was 1%, resulting in gel composition 11.

[0077] Experimental group 12 The preparation method was the same as that of experimental group 5, except that the total amount of CS and HA added was 3%, resulting in gel composition 12.

[0078] Experimental group 13 The preparation method was the same as that of experimental group 5, except that the amount of pectin added was 8%, resulting in gel composition 13.

[0079] Experimental group 14 The preparation method was the same as that of experimental group 5, except that the amount of pectin added was 6%, resulting in gel composition 14.

[0080] Experimental group 15 The preparation method is the same as that of experimental group 5, except that the molecular weight of HA is 30-50kDa, resulting in gel composition 15.

[0081] Experimental group 16 The preparation method was the same as that of experimental group 5, except that the molecular weight of HA was 5 kDa, resulting in gel composition 16.

[0082] Experimental group 17 The preparation method was the same as that of experimental group 5, except that the molecular weight of CS was 10kDa, resulting in gel composition 17.

[0083] Experimental group 18 The preparation method was the same as that of experimental group 5, except that the molecular weight of CS was 5kDa, resulting in gel composition 18.

[0084] Experimental group 19 1. Weigh out CS (molecular weight 30-50kDa) and HA powder (molecular weight 200-400kDa) separately, with a mass ratio of 2:1. The total amount of CS and HA added is 2%. Add pure water and heat and stir at 50°C until completely dissolved. 2. Add 10% ZEN, heat and stir until completely dissolved without any powder lumps, cool and solidify to obtain gel composition 19.

[0085] Experimental group 20 1. Weigh out CS (molecular weight 30-50kDa) and HA powder (molecular weight 200-400kDa) separately, with a mass ratio of 2:1. The total amount of CS and HA added is 2%. Add pure water and heat and stir at 50°C until completely dissolved. 2. Add 10% agar, heat and stir until completely dissolved without any powder lumps, cool and solidify to obtain gel composition 20.

[0086] Experimental group 21 1. Weigh 2% acetylated HA powder (molecular weight 30-50kDa), add pure water and heat and stir at 50℃ until completely dissolved; 2. Add 10% pectin, heat and stir until completely dissolved without any powder lumps, cool and solidify to obtain gel composition 21.

[0087] Experimental group 22 CS (molecular weight 30-50kDa) and acetylated HA powder (molecular weight 30-50kDa) were weighed separately, with a mass ratio of 2:1. The total amount of CS and HA added was 2%. Pure water was added and heated and stirred at 50°C until completely dissolved. After cooling and solidification, gel composition 22 was obtained.

[0088] Experimental group 23 1. Weigh out CS (molecular weight 30-50kDa) and acetylated HA powder (molecular weight 30-50kDa) separately, with a mass ratio of 2:1. The total amount of CS and HA added is 2%. Add pure water and heat and stir at 50°C until completely dissolved. 2. Add 10% pectin, heat and stir until completely dissolved without any powder lumps, cool and solidify to obtain gel composition 23.

[0089] Experimental group 24 1. Weigh out CS (molecular weight 30-50kDa) and HA powder (molecular weight 200-400kDa) separately, with a mass ratio of 2:1. The total amount of CS and HA added is 2%. Add pure water and heat and stir at 50°C until completely dissolved. 2. Add 8% pectin and 0.5% carbomer, heat and stir until completely dissolved without any powder lumps, cool and solidify to obtain gel composition 24.

[0090] Experimental group 25 1. Weigh out CS (molecular weight 30-50kDa) and HA powder (molecular weight 200-400kDa) separately, with a mass ratio of 2:1. The total amount of CS and HA added is 2%. Add pure water and heat and stir at 50°C until completely dissolved. 2. Add 10% pectin and 0.5% carbomer, heat and stir until completely dissolved without any powder lumps, cool and solidify to obtain gel composition 25.

[0091] Test case Routine shower environment experimental setup: A water inlet pipe was connected to a bottle cap with an opening, and a filter cloth was wrapped around the upper side wall (to prevent gel fragments from flowing out), creating a "top-in, side-out" water outlet tank. The gel composition (approximately 100 g) was placed inside the tank, and a peristaltic pump was connected. Tap water continuously entered the tank through the inlet pipe, rinsing the gel composition, and then the solution water (tap water containing the gel composition solute) flowed out through the side hole. During the experiment, the tap water flow rate was controlled at 6.50 L / min, and the rinsing water temperature was maintained at 40 ℃ to simulate a normal rinsing environment.

[0092] Gel film formation test method: Using the above-mentioned routine rinsing environment experimental device, the gel compositions of experimental groups 2, 3, 5, 9, 18, 19, and 20 were placed in the device and continuously rinsed with tap water for 30 seconds. After rinsing, the residual moisture on the surface of the imitation leather was left, and it was placed directly at room temperature to air dry without wiping with paper towels. After air drying, the surface of the imitation leather was observed using a scanning electron microscope (SEM) to evaluate the film formation state and film quality of each gel block on the surface of the imitation leather after rinsing.

[0093] The criteria for judging the film-forming effect are as follows: The core indicators are the amount of attached material, the continuity and integrity of the membrane, and the overall judgment is based on the density of the membrane.

[0094] Advantages: The surface of the imitation leather has a large number of attached substances, which form a dense, sheet-like film that completely covers the substrate, so the texture of the imitation leather is not visible.

[0095] Good: The surface of the imitation leather has a lot of attached substances, which form a dense, sheet-like film with slight gaps in some areas, but no large-area damage.

[0096] Poor: The surface of the imitation leather has a small amount of attached material, the film layer is discontinuous and fragmented, with only localized dot-like residues and no complete film structure.

[0097] No film formation: The surface of the imitation leather has no or only scattered attachments, no film layer coverage, and no continuous film structure.

[0098] The test results are shown in Table 2 and Figure 1-9 As shown.

[0099] Table 2

[0100] The results showed that composition 5 could improve the skin retention of chondroitin sulfate and hyaluronic acid, enabling chondroitin sulfate and hyaluronic acid to be effectively deposited on the skin surface during rinsing, ensuring that they can be effectively deposited on the skin surface and form a protective film.

[0101] Methods for testing the moisture content of the stratum corneum: The Corneometer® skin moisture test is used to determine the hydration level (i.e., water content) of the stratum corneum of the skin.

[0102] (1) No products should be used on the inner forearm test site for 2-3 days before the test, and it should not come into contact with water for 1-2 hours. Before the test, the subject should clean the inner forearm by wiping it with a dry tissue. Mark the test area with an area of ​​at least 3cm×3cm and a 1cm interval between areas. After sitting quietly in the laboratory for 30 minutes to adapt, start testing the initial value of skin moisture content (test room temperature and humidity: 21℃, 51%). Each area should be measured in parallel at least 3 times, and the measuring probe should be cleaned between two measurements.

[0103] (2) Cleaning procedure: Cleaning was performed using the above-mentioned daily shower environment experimental apparatus. The PC group was rinsed with 40°C tap water for 40 seconds without the application of shower gel (commercially available product, surfactant system is a combination of soap base + amphoteric + sulfate and other surfactants); the NC group followed the same cleaning procedure as the experimental group, but the rinsing solution was different (the NC group used 40°C tap water, while the experimental group used a 40°C solution containing gel composition 5). After rinsing the test skin area with vertical water flow for 10 seconds, 200 pL of shower gel was dropped into the center of the area, sterile nitrile gloves were worn, and the area was rubbed in a circular motion for 1 minute, followed by rinsing for 30 seconds to ensure thorough rinsing, and then gently patted dry with a paper towel. Each group was repeated 3 times.

[0104] Specifically, the cleaning methods for different groups are shown in Table 3.

[0105] Table 3 Cleaning Methods

[0106] (3) Measurement after cleaning: The skin moisture content of the subjects was measured 2 hours after cleaning.

[0107] (4) Data processing: The change in stratum corneum moisture content (Δstratum corneum moisture content) is defined as the difference between the measured value after cleaning and the initial measured value before cleaning. ΔCoverage Moisture Content = Post-cleaning Measurement Value – Initial Measurement Value Before Cleaning A negative Δ value indicates a decrease in skin moisture content, while a positive Δ value indicates an increase in skin moisture content.

[0108] Results and statistical analysis: t-tests were used for comparisons between groups. A p-value < 0.05 was considered statistically significant; groups with the same letter were not considered significantly different. Test results are shown in Table 4. Figure 10 As shown.

[0109] Criteria for judging efficacy: a. Δ is greater than Δ NC If the sample group shows a significant difference from the NC group, it can effectively improve skin problems such as reduced skin moisture and barrier damage caused by the irritation and / or damage to the skin caused by the cleaning product during the washing process. b. Δ is greater than Δ 单体 If the sample group shows significant differences from the monomer group, then the sample group has a significant synergistic effect and can better improve the skin moisture reduction and barrier damage caused by the skin irritation and / or damage of the cleaning product during the washing process. c. If b is satisfied and there is no significant difference from the PC group, then the sample group has a superior effect.

[0110] Table 4. Summary of intergroup comparisons of changes in cuticle moisture content in different treatment groups

[0111] Note: The monomers compared in experimental groups 4-7, 11-13, 15-18, 24, and 25 refer to experimental groups 1, 2, and 3; the monomers compared in experimental group 23 refer to experimental groups 1, 2, and 21. The results show: 1. The stratum corneum moisture content of the skin in the PC group decreased slightly, which may be due to the effect of lipid disturbance in the stratum corneum caused by hot water.

[0112] 2. The NC group showed a significant decrease in stratum corneum moisture content, with a significant change in the Δ value.

[0113] 3. There were significant differences between experimental groups 1 and 2 and the NC group, but there was no significant difference between experimental groups 1 and 2, indicating that chondroitin sulfate did not play a role in further increasing skin hydration.

[0114] 4. Experimental groups 4-7, 11-13, 15, 17, and 23-25 ​​showed significant differences compared to the NC group, indicating that the product effectively reduces skin irritation and / or damage caused by cleansing products, leading to skin problems such as decreased skin moisture and barrier damage. Compared to the single-component group, the product also showed significant differences, indicating a further synergistic effect. In particular, experimental groups 5 and 12 showed no difference compared to the PC group, indicating that the product can restore skin moisture to the level before the use of cleansing products, completely eliminating the impact of cleansing products on the skin during the washing process.

[0115] TEWL testing method: The method is consistent with the method for testing the moisture content of the stratum corneum, as follows: The Corneometer® Skin Moisture Tester is used to determine the moisture loss from the stratum corneum of the skin (i.e., transepidermal water loss).

[0116] (1) No products should be used on the inner forearm test site for 2-3 days before the test, and it should not come into contact with water for 1-2 hours. Before the test, the subject should clean the inner forearm by wiping it with a dry tissue. Mark the test area with an area of ​​at least 3cm×3cm and a 1cm interval between areas. After sitting quietly in the laboratory for 30 minutes to adapt, start testing the initial value of the skin TEWL (test room temperature and humidity: 21℃, 51%). Each area should be measured in parallel at least 3 times, and the measuring probe should be cleaned between two measurements. This is the Pre value.

[0117] (2) Cleaning Procedure: Cleaning was performed using the same daily shower environment test apparatus as described above. The NC group rinsed with 40°C tap water for 40 seconds and then applied shower gel (commercially available product, surfactant system consisting of soap base + amphoteric + sulfate and other surfactants). The Case 5 group followed the same cleaning procedure as the NC group, but with a different rinsing solution (the NC group used 40°C tap water, while the Case 5 group used a 40°C solution containing gel composition 5). After rinsing the test skin area with vertical water flow for 10 seconds, 200 pL of shower gel was applied to the center of the area. Wearing sterile nitrile gloves, the area was massaged in circular motions for 1 minute, followed by rinsing for 30 seconds to ensure thorough cleaning. The area was then gently patted dry with a paper towel. Each group was repeated 3 times.

[0118] (3) After sitting still for 0.5 hours, the skin TEWL value is measured with a probe, which is the 0.5-hour value.

[0119] Specifically, the cleaning methods for different groups are shown in Table 5.

[0120] Table 5 Cleaning Methods

[0121] (4) Measurement after cleaning: The skin TEWL value of the subjects was measured 0.5 h after cleaning.

[0122] (5) Statistical analysis of results: t-tests were used for comparisons between groups. A p-value < 0.05 was considered statistically significant, and ** was used to indicate the significance. The test results are shown in Table 6 and... Figure 11 As shown.

[0123] Table 6 TEWL Test Results

[0124] The results show: The TEWL in the NC group increased significantly after washing compared to before washing, indicating a decrease in skin barrier function after washing. In the case 5 group, the TEWL decreased significantly after washing compared to before washing, indicating an improvement in skin barrier function after washing.

Claims

1. A gel composition comprising: (a) At least one sulfated glycosaminoglycan with a molecular weight of 8-100 kDa; (b) Select at least one from the group consisting of hyaluronic acid or its salts with a molecular weight of 20-1000 kDa, acetylated hyaluronic acid or its salts; and (c) Pectin, The mass ratio of (a) to (b) is 0.1:1 to 7:

1.

2. The gel composition according to claim 1, wherein the mass ratio of (a) to (b) is 0.5:1 to 6.5:1; Preferably, the mass ratio of (c) to the sum of the masses of (a) and (b) is 3.3:1-15:

1.

3. The gel composition according to claim 1 or 2, wherein in the gel composition, the content of (c) is greater than 6% and less than 30%.

4. The gel composition according to any one of claims 1-3, wherein the molecular weight of (a) is 8-70 kDa; Preferably, the molecular weight of (b) is 20-800 kDa.

5. The gel composition according to any one of claims 1-3, wherein, The sulfated glycosaminoglycans include one or more of chondroitin sulfate, dermatan sulfate, keratin sulfate, heparin, and heparan sulfate.

6. The gel composition according to any one of claims 1-5, wherein the total content of (a) and (b) in the gel composition is 0.5% or more.

7. Use of the gel composition of any one of claims 1-6 in improving the retention of (a) and (b) on the skin surface under water flow conditions.

8. The use according to claim 7, wherein the gel composition is used to improve skin dryness after washing.

9. The gel composition according to any one of claims 1-6 is used to reduce skin irritation and / or damage caused by cleansing products.

10. Use of the gel composition according to any one of claims 1-6 in the preparation of daily chemical products.

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