Instant clear voice lung moistening tea freeze-dried powder and preparation method thereof

Abstract

The application belongs to the field of medicine and health food, and particularly relates to a quick-dissolving Qingyin Yufei tea freeze-dried powder and a preparation method thereof. The freeze-dried powder contains Herba Scaphium scaphium, Radix Adenophorae, Fried Cassia Seed, Cantharis, Flos Lonicerae, Radix Ophiopogonis and a freeze-dried excipient, has the characteristics of quick-dissolving and good taste, and improves the carrying convenience and patient compliance. Meanwhile, the preparation method of the freeze-dried powder is simple and easy to operate, parameters are controllable, and industrialized production can be easily realized.

Description

Technical Field

[0001] This invention belongs to the field of medicine and health food, specifically relating to an instant-dissolving, lung-clearing, and remedy-moistening freeze-dried tea powder and its preparation method. Background Technology

[0002] Common symptoms of throat ailments include dryness, itching, pain, a feeling of obstruction, redness and swelling of the throat, hoarseness, and difficulty breathing. These symptoms are most common in winter and spring, often secondary to acute sinusitis, acute rhinitis, or acute tonsillitis, and are frequently complications of infectious diseases such as measles, influenza, and scarlet fever. Factors such as exposure to cold, fatigue, prolonged exposure to chemical gases or dust, and long-term smoking can lower the body's resistance, making it more susceptible to the disease. Adults primarily experience throat symptoms, initially dryness, itching, and burning, gradually progressing to pain that worsens with swallowing. Weak adults or children may experience significant systemic symptoms such as fever, headache, chills, loss of appetite, and aches and pains in the limbs.

[0003] Traditional Chinese medicine theory attributes these symptoms to "depletion of body fluids and malnourishment of the lungs." Specifically, the lungs are a delicate organ, preferring moisture and disliking dryness. They govern qi and respiration, and their meridians connect to the throat. When various internal and external factors, such as prolonged illness damaging yin, overexertion, external dryness, or improper voice use, deplete the body's lung and kidney yin fluids, leading to insufficient yin fluids, the throat and lungs lose nourishment. When yin fails to control yang, it easily generates upward flaring of deficient fire, further damaging body fluids and creating a vicious cycle of dryness. Therefore, generating fluids to moisten dryness, clearing the voice and benefiting the throat, and nourishing yin and clearing the lungs become the basic principles for treating these symptoms. The aim is to replenish depleted body fluids, clear deficient fire, and restore the normal nourishment and physiological function of the throat and lungs.

[0004] Several traditional Chinese medicine compound preparations already exist in the technology. "Jin Sang Qing Yin Capsules" are composed of sixteen Chinese herbs, including Scrophularia, Rehmannia, Ophiopogon, Scutellaria, Moutan, Paeonia lactiflora, Fritillaria cirrhosa, Alisma, stir-fried Coix lacryma-jobi, Dendrobium, stir-fried Bombyx mori, Mentha, Sterculia lychnophora, Cicadae periostracum, Oroxylum indicum, and Glycyrrhiza uralensis. They are said to nourish Yin, clear the lungs, resolve phlegm, and soothe the throat. However, capsules require multiple steps, including disintegration, drug dissolution, and gastrointestinal absorption, to be effective. "Qing Yin Wan," on the other hand, contains Terminalia chebula, Fritillaria cirrhosa, Baiyao Jian, Prunus mume, Pueraria lobata, Poria cocos, Glycyrrhiza uralensis, and Trichosanthes kirilowii. While it can relieve throat discomfort, its dissolution rate is slow and its onset of action is also slow. For patients experiencing dry and itchy throat due to depletion of body fluids, these preparations not only fail to provide immediate relief but also fail to achieve rapid release of the active ingredients, thus failing to achieve the significant effects of generating fluids, moistening dryness, clearing the voice, and nourishing the lungs.

[0005] Existing formulations still have the following drawbacks: First, traditional preparation processes have limited ability to break down the cell walls of medicinal materials, resulting in the failure to fully extract and release the active ingredients directly related to "generating body fluids and moisturizing dryness," which affects the product's dissolution and bioavailability. Second, in terms of product experience, capsules can cause secondary irritation to patients with symptoms such as dryness and itching in the throat, while pills are often bitter and have a poor taste, leading to low patient compliance.

[0006] In summary, existing traditional Chinese medicine preparations for clearing the voice and moistening the lungs generally suffer from drawbacks such as slow dissolution, low bioavailability, poor taste, and poor patient compliance. Therefore, overcoming these shortcomings and developing a novel product that can rapidly and efficiently produce the effects of "generating body fluids and moistening dryness, clearing the voice and moistening the lungs," while also being portable and having a pleasant taste, has become a pressing technical problem to be solved in this field. Summary of the Invention

[0007] In view of the shortcomings of existing technologies, this invention provides a fast-dissolving, lung-moistening, and voice-clearing freeze-dried powder and its preparation method. The resulting freeze-dried powder is fast-dissolving and has a good taste, improving portability and patient compliance. Furthermore, the preparation method of this freeze-dried powder is simple to operate, with controllable parameters, making it easy to achieve industrial production.

[0008] The inventors of this application systematically studied the prescription components and preparation process, aiming to develop a tea freeze-dried powder that combines the effects of clearing the voice and moisturizing the lungs with the advantage of cold solubility. The inventors discovered that using only traditional lung-moistening medicinal materials such as Malva nut, honeysuckle, Ophiopogon japonicus, Terminalia chebula, and Cassia seed, although possessing basic throat-clearing and moisturizing functions, results in a bitter tea soup with poor solubility at low temperatures, failing to meet the product's requirements for taste and convenience.

[0009] To address the aforementioned issues, the inventors added Adenophora stricta to the formula and unexpectedly discovered that its extract not only significantly improved the aftertaste of the tea and reduced bitterness, but also enhanced the anti-inflammatory and antibacterial activity of the composition, thereby synergistically improving the overall lung-clearing and soothing effects. To further enhance the product's taste and portability, the inventors added erythritol, allulose, or steviol glycosides, comprehensively optimizing the product's flavor and quality.

[0010] The specific contents of this invention are as follows: On one hand, the present invention provides an instant-dissolving lung-clearing and soothing tea freeze-dried powder, wherein the freeze-dried powder contains malva nut, sand ginseng, roasted cassia seed, green plum, honeysuckle, ophiopogon japonicus and freeze-drying excipients. After the components are mixed into a liquid, the freeze-dried powder is obtained by freeze-drying. In the freeze-dried powder, the freeze-drying excipients are selected from any one or more of erythritol, allulose and steviol glycosides.

[0011] Preferably, in the freeze-dried powder, the weight parts of Malva nut are 25-90 parts, the weight parts of Adenophora stricta are 70-100 parts, the weight parts of stir-fried Cassia tora seeds are 120-190 parts, the weight parts of Terminalia chebula are 30-70 parts, the weight parts of Lonicera japonica are 50-110 parts, and the weight parts of Ophiopogon japonicus are 30-110 parts.

[0012] More preferably, in the freeze-dried powder, the weight parts of Malva nut are 26 parts, the weight parts of Adenophora stricta are 80 parts, the weight parts of stir-fried Cassia tora seeds are 160 parts, the weight parts of Terminalia chebula are 40 parts, the weight parts of honeysuckle are 80 parts, and the weight parts of Ophiopogon japonicus are 80 parts.

[0013] Preferably, the freeze-dried powder contains 5 to 20 parts by weight of the freeze-drying excipient; more preferably, the freeze-drying excipient contains 5 to 10 parts by weight.

[0014] The instant-dissolving lung-clearing and soothing tea freeze-dried powder can be made using conventional methods. It can be taken by dissolving it directly in cool boiled water or by mixing it into food or beverages. These methods are not intended to limit the scope of protection of this invention.

[0015] Preferably, the present invention provides a method for preparing an instant-dissolving, lung-clearing, and moisturizing freeze-dried tea powder, the method comprising the following steps: A. Pulverize the Chinese medicinal herbs Malva nut, honeysuckle, Adenophora stricta, and stir-fried cassia seed separately. Add the pulverized Malva nut to 20-30% ethanol (v / v) and extract with ultrasound-assisted extraction at room temperature. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Malva nut extract. Add the pulverized honeysuckle to 60-75% ethanol (v / v) and extract with ultrasound-assisted heating. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain honeysuckle extract. Add the pulverized Adenophora stricta to purified water and extract with ultrasound-assisted extraction at low temperature. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Adenophora stricta extract. Add the pulverized stir-fried cassia seed to 40-55% ethanol (v / v) and extract with ultrasound-assisted heating. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Adenophora stricta extract. B. After removing the pits from the green plums, crush them and then extract them with 40-60% ethanol by volume. The extract is dried with infrared radiation-assisted drying while being stirred to obtain green plum extract. C. After pulverizing and vacuum drying, the Ophiopogon japonicus was separated and extracted by countercurrent chromatography, and concentrated by nanofiltration to obtain the enriched Ophiopogon japonicus components; D. Take the extracts of the above components, add them to ethanol with a volume fraction of 30-40%, stir, add lyophilization excipients, filter through a 0.22μm microporous membrane for sterilization, dispense into vials, and freeze-dry to obtain instant-dissolving Qingyin Runfei Tea lyophilized powder.

[0016] Preferably, in the method, in step A, the Chinese medicinal materials are pulverized to 40-80 mesh.

[0017] Preferably, in the method, during step A, when extracting Malva nut, the ultrasonic frequency is 25~30kHz and the time is 10~20min.

[0018] Preferably, in the method, during step A, when extracting honeysuckle, the ultrasonic frequency is 20~25kHz, the temperature is 40~45℃, and the time is 30~50min.

[0019] Preferably, in the method, during step A, when extracting Adenophora stricta, the ultrasonic frequency is 30~35kHz, the temperature is 10~20℃, and the time is 30~40min.

[0020] Preferably, in the method, during step A, when extracting roasted cassia seeds, the ultrasonic frequency is 35~40kHz, the temperature is 55~60℃, and the time is 40~60min.

[0021] Preferably, in the method, in step B, the Chinese medicinal materials are pulverized to 40-80 mesh.

[0022] Preferably, in the method, in step B, the infrared radiation drying time is 15-20 min, the infrared radiation physical field parameters are set to a long-wave infrared wavelength range of 5-9 μm, the heating temperature is set to 35-45℃, and the distance between the infrared lamp and the extract is 20-30 cm.

[0023] Preferably, in the method, in step C, the Ophiopogon japonicus is pulverized to 40-80 mesh.

[0024] Preferably, in the method, in step C, the vacuum drying process involves heating to 40-45°C for 20-24 hours.

[0025] Preferably, in the method, in step C, the solvent system is n-butanol-water with a volume ratio of 4:6 and a flow rate of 2 mL / min.

[0026] Preferably, in the method, in step C, the molecular weight cutoff of the nanofiltration membrane is 500~1000 Da.

[0027] Preferably, in the method, step D, the freeze-drying step is as follows: the pre-freezing temperature is -40~-45℃, maintained for 3~4h; the sublimation drying temperature is -20~-15℃, maintained for 2~3h; and the desorption drying temperature is 20~35℃, maintained for 4~6h.

[0028] Compared with the prior art, the present invention has the following outstanding advantages: 1. This invention provides a freeze-dried powder for clearing the voice and moisturizing the lungs, which has the effects of promoting body fluid production and moisturizing dryness, clearing the voice and moisturizing the lungs, and also has the characteristics of good taste and easy portability.

[0029] 2. Compared with the prior art, the Qingyin Runfei Tea freeze-dried powder prepared by the present invention has significantly improved in terms of taste, solubility, anti-inflammatory and bactericidal activity and stability; moreover, it does not require the addition of excipients such as sucrose, which are not conducive to people with blood sugar control. The raw materials and excipients used are highly safe and have mild medicinal properties, which meets the requirements of health food that can be used as both medicine and food.

[0030] 3. In the preparation method of this invention, ultrasonic-assisted alcohol extraction or water extraction is used, which is more conducive to protecting the volatile oils and heat-sensitive components in Malva nut and honeysuckle, as well as the polysaccharides in Adenophora stricta. For Cassia tora seeds with hard texture, its effective component anthraquinone glycosides are also extracted efficiently. The use of soaking combined with infrared radiation-assisted drying can efficiently extract and protect the volatile oils and heat-sensitive flavonoids in green plums. The use of vacuum drying combined with countercurrent chromatography can efficiently extract and enrich the polysaccharides and volatile oils in Ophiopogon japonicus.

[0031] 4. The preparation method of the present invention is simple to operate, the process parameters of each step are easy to control, no complicated special equipment is required, which significantly reduces the production difficulty and production cost, and is suitable for large-scale industrial production. Detailed Implementation

[0032] The following specific embodiments further illustrate the present invention, but do not limit the scope of the present invention in any way. Those skilled in the art can make various modifications or improvements based on the basic idea of ​​the present invention, but as long as they do not depart from the basic idea of ​​the present invention, they are all within the scope of the present invention.

[0033] Example 1 Prescription components and dosages: 25 parts by weight of Malva nut, 70 parts by weight of Adenophora stricta, 120 parts by weight of stir-fried Cassia seed, 30 parts by weight of Terminalia chebula, 50 parts by weight of Lonicera japonica, 30 parts by weight of Ophiopogon japonicus, and 5 parts by weight of erythritol.

[0034] Preparation method: A. Grind the prescribed amounts of the Chinese medicinal herbs, including Malva nut, honeysuckle, Adenophora stricta, and stir-fried Cassia tora, into 40-60 mesh. Add the ground Malva nut to 20% ethanol (v / v), and perform ultrasonic-assisted extraction at room temperature at a frequency of 25 kHz for 10 minutes. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Malva nut extract. Add the ground honeysuckle to 60% ethanol (v / v), and perform ultrasonic-assisted heating extraction at a frequency of 20 kHz and a temperature of 40-42℃ for 30 minutes. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Malva nut extract. Collect the supernatant and concentrate under reduced pressure to obtain honeysuckle extract; add pulverized ginseng to purified water and extract under low temperature with ultrasonic assistance at a frequency of 30 kHz, a temperature of 10-12℃, and a time of 30 min. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain ginseng extract; add pulverized roasted cassia seeds to 40% ethanol and extract under ultrasonic assistance with heating at a frequency of 35 kHz, a temperature of 55-57℃, and a time of 40 min. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain ginseng extract. B. After removing the pits from the prescribed amount of green plums, pulverize them to 60-80 mesh, then extract them with 40% ethanol (by volume). Dry the extract with infrared radiation while stirring. The infrared radiation drying time is 15 minutes. The infrared radiation physical field parameters are set to a long-wave infrared wavelength range of 5-9 μm, a heating temperature of 35-45℃, and a distance of 30 cm between the infrared lamp and the extract to obtain green plum extract. C. The prescribed amount of Ophiopogon japonicus was pulverized to 40-60 mesh and then vacuum dried. During drying, the temperature was heated to 40-45℃ for 20 hours. Then, the mixture was separated and extracted by countercurrent chromatography. The solvent system was n-butanol-water with a volume ratio of 4:6 and a flow rate of 2 mL / min. The mixture was concentrated by nanofiltration. The molecular weight cutoff of the nanofiltration membrane was 500-1000 Da, and the enriched extract of Ophiopogon japonicus was obtained. D. Take the extracts of the aforementioned components, add them to 30% ethanol (by volume), stir, add the lyophilization excipient, filter through a 0.22μm microporous membrane for sterilization, dispense into vials, and freeze-dry. The steps are as follows: pre-freezing temperature is -40~-45℃, maintained for 3h; sublimation drying temperature is -20~-15℃, maintained for 2h; and desorption drying temperature is 20~35℃, maintained for 4h, to obtain the final product.

[0035] Example 2: Prescription components and dosages: 90 parts by weight of Malva nut, 100 parts by weight of Adenophora stricta, 190 parts by weight of stir-fried Cassia seed, 70 parts by weight of Terminalia chebula, 110 parts by weight of honeysuckle, 110 parts by weight of Ophiopogon japonicus, and 10 parts each of allulose and steviol glycosides.

[0036] Preparation method: A. Grind the prescribed amounts of the Chinese medicinal herbs, including Malva nut, honeysuckle, Adenophora stricta, and stir-fried Cassia tora, into 60-80 mesh. Add the ground Malva nut to 30% ethanol (v / v), and perform ultrasound-assisted extraction at room temperature at a frequency of 30 kHz for 20 min. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Malva nut extract. Add the ground honeysuckle to 75% ethanol (v / v), and perform ultrasound-assisted heating extraction at a frequency of 25 kHz and a temperature of 43-45℃ for 50 min. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Malva nut extract. Collect the supernatant and concentrate under reduced pressure to obtain honeysuckle extract; add pulverized ginseng to purified water and extract under low temperature with ultrasound-assisted extraction at a frequency of 35 kHz, a temperature of 18-20℃, and a time of 40 min. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain ginseng extract; add pulverized roasted cassia seeds to 55% ethanol and extract under ultrasound-assisted heating at a frequency of 40 kHz, a temperature of 58-60℃, and a time of 60 min. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain ginseng extract. B. After removing the pits from the prescribed amount of green plums, pulverize them to 40-60 mesh, then extract them with 60% ethanol (by volume). Dry the extract with infrared radiation while stirring. The infrared radiation drying time is 20 minutes. The infrared radiation physical field parameters are set to a long-wave infrared wavelength range of 5-9 μm, a heating temperature of 35-45℃, and a distance of 20 cm between the infrared lamp and the extract to obtain green plum extract. C. The prescribed amount of Ophiopogon japonicus was pulverized to 60-80 mesh and then vacuum dried. During drying, the temperature was heated to 40-45℃ for 24 hours. Then, the mixture was separated and extracted by countercurrent chromatography. The solvent system was n-butanol-water with a volume ratio of 4:6 and a flow rate of 2 mL / min. The mixture was concentrated by nanofiltration. The molecular weight cutoff of the nanofiltration membrane was 500-1000 Da, and the enriched extract of Ophiopogon japonicus was obtained. D. Take the extracts of the aforementioned components, add them to 40% ethanol (by volume), stir, add the lyophilization excipient, filter through a 0.22μm microporous membrane for sterilization, dispense into vials, and freeze-dry. The steps are as follows: pre-freezing temperature is -40~-45℃, maintained for 4h; sublimation drying temperature is -20~-15℃, maintained for 3h; and desorption drying temperature is 20~35℃, maintained for 6h, to obtain the final product.

[0037] Comparative Example 1 Prescription components and dosage: 25 parts by weight of Malva nut, 120 parts by weight of stir-fried cassia seed, 30 parts by weight of Terminalia chebula, 50 parts by weight of honeysuckle, 30 parts by weight of Ophiopogon japonicus, and 5 parts by weight of erythritol.

[0038] Preparation method: A. Grind the prescribed amounts of the Chinese medicinal herbs, including Malva nut, honeysuckle, and stir-fried cassia seed, into 40-60 mesh. Add the ground Malva nut to 20% ethanol and extract using ultrasound-assisted extraction at room temperature at a frequency of 25 kHz for 10 minutes. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Malva nut extract. Add the ground honeysuckle to 60% ethanol and extract using ultrasound-assisted heating at a frequency of 20 kHz, a temperature of 40-42℃, for 30 minutes. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain honeysuckle extract. Add the ground stir-fried cassia seed to 40% ethanol and extract using ultrasound-assisted heating at a frequency of 35 kHz, a temperature of 55-57℃, for 40 minutes. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain glehnia root extract. B. After removing the pits from the prescribed amount of green plums, pulverize them to 60-80 mesh, then extract them with 40% ethanol (by volume). Dry the extract with infrared radiation while stirring. The infrared radiation drying time is 15 minutes. The infrared radiation physical field parameters are set to a long-wave infrared wavelength range of 5-9 μm, a heating temperature of 35-45℃, and a distance of 30 cm between the infrared lamp and the extract to obtain green plum extract. C. The prescribed amount of Ophiopogon japonicus was pulverized to 40-60 mesh and then vacuum dried. During drying, the temperature was heated to 40-45℃ for 20 hours. Then, the mixture was separated and extracted by countercurrent chromatography. The solvent system was n-butanol-water with a volume ratio of 4:6 and a flow rate of 2 mL / min. The mixture was concentrated by nanofiltration. The molecular weight cutoff of the nanofiltration membrane was 500-1000 Da, and the enriched extract of Ophiopogon japonicus was obtained. D. Take the extracts of the aforementioned components, add them to 30% ethanol (by volume), stir, add the lyophilization excipient, filter through a 0.22μm microporous membrane for sterilization, dispense into vials, and freeze-dry. The steps are as follows: pre-freezing temperature is -40~-45℃, maintained for 3h; sublimation drying temperature is -20~-15℃, maintained for 2h; and desorption drying temperature is 20~35℃, maintained for 4h, to obtain the final product.

[0039] Comparative Example 2 Prescription components and dosages: 25 parts by weight of Malva nut, 70 parts by weight of Adenophora stricta, 120 parts by weight of stir-fried Cassia seed, 30 parts by weight of Terminalia chebula, 50 parts by weight of honeysuckle, 30 parts by weight of Ophiopogon japonicus, and 5 parts by weight of mannitol.

[0040] Preparation method: A. Grind the prescribed amounts of the Chinese medicinal herbs, including Malva nut, honeysuckle, Adenophora stricta, and stir-fried Cassia tora, into 40-60 mesh. Add the ground Malva nut to 20% ethanol (v / v), and perform ultrasonic-assisted extraction at room temperature at a frequency of 25 kHz for 10 minutes. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Malva nut extract. Add the ground honeysuckle to 60% ethanol (v / v), and perform ultrasonic-assisted heating extraction at a frequency of 20 kHz and a temperature of 40-42℃ for 30 minutes. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Malva nut extract. Collect the supernatant and concentrate under reduced pressure to obtain honeysuckle extract; add pulverized ginseng to purified water and extract under low temperature with ultrasonic assistance at a frequency of 30 kHz, a temperature of 10-12℃, and a time of 30 min. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain ginseng extract; add pulverized roasted cassia seeds to 40% ethanol and extract under ultrasonic assistance with heating at a frequency of 35 kHz, a temperature of 55-57℃, and a time of 40 min. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain ginseng extract. B. After removing the pits from the prescribed amount of green plums, pulverize them to 60-80 mesh, then extract them with 40% ethanol (by volume). Dry the extract with infrared radiation while stirring. The infrared radiation drying time is 15 minutes. The infrared radiation physical field parameters are set to a long-wave infrared wavelength range of 5-9 μm, a heating temperature of 35-45℃, and a distance of 30 cm between the infrared lamp and the extract to obtain green plum extract. C. The prescribed amount of Ophiopogon japonicus was pulverized to 40-60 mesh and then vacuum dried. During drying, the temperature was heated to 40-45℃ for 20 hours. Then, the mixture was separated and extracted by countercurrent chromatography. The solvent system was n-butanol-water with a volume ratio of 4:6 and a flow rate of 2 mL / min. The mixture was concentrated by nanofiltration. The molecular weight cutoff of the nanofiltration membrane was 500-1000 Da, and the enriched extract of Ophiopogon japonicus was obtained. D. Take the extracts of the aforementioned components, add them to 30% ethanol (by volume), stir, add the lyophilization excipient, filter through a 0.22μm microporous membrane for sterilization, dispense into vials, and freeze-dry. The steps are as follows: pre-freezing temperature is -40~-45℃, maintained for 3h; sublimation drying temperature is -20~-15℃, maintained for 2h; and desorption drying temperature is 20~35℃, maintained for 4h, to obtain the final product.

[0041] Comparative Example 3 Prescription components and dosages: 25 parts by weight of Malva nut, 70 parts by weight of Adenophora stricta, 120 parts by weight of stir-fried Cassia seed, 30 parts by weight of Terminalia chebula, 50 parts by weight of Lonicera japonica, 30 parts by weight of Ophiopogon japonicus, and 5 parts by weight of erythritol.

[0042] Preparation method: A. Grind the prescribed amounts of the Chinese medicinal herbs, including Malva nut, honeysuckle, Adenophora stricta, and stir-fried cassia seed, into 40-60 mesh. Add the ground Malva nut to 20% ethanol (v / v), extract at room temperature, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Malva nut extract. Add the ground honeysuckle to 60% ethanol (v / v), extract, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain honeysuckle extract. Add the ground Adenophora stricta to purified water, extract at room temperature, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Adenophora stricta extract. Add the ground stir-fried cassia seed to 40% ethanol (v / v), extract at room temperature, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Adenophora stricta extract. B. After removing the pits from the prescribed amount of green plums, pulverize them to 60-80 mesh, then extract them with 40% ethanol (by volume). Dry the extract with infrared radiation while stirring. The infrared radiation drying time is 15 minutes. The infrared radiation physical field parameters are set to a long-wave infrared wavelength range of 5-9 μm, a heating temperature of 35-45℃, and a distance of 30 cm between the infrared lamp and the extract to obtain green plum extract. C. The prescribed amount of Ophiopogon japonicus was pulverized to 40-60 mesh and then vacuum dried. During drying, the temperature was heated to 40-45℃ for 20 hours. Then, the mixture was separated and extracted by countercurrent chromatography. The solvent system was n-butanol-water with a volume ratio of 4:6 and a flow rate of 2 mL / min. The mixture was concentrated by nanofiltration. The molecular weight cutoff of the nanofiltration membrane was 500-1000 Da, and the enriched extract of Ophiopogon japonicus was obtained. D. Take the extracts of the aforementioned components, add them to 30% ethanol (by volume), stir, add the lyophilization excipient, filter through a 0.22μm microporous membrane for sterilization, dispense into vials, and freeze-dry. The steps are as follows: pre-freezing temperature is -40~-45℃, maintained for 3h; sublimation drying temperature is -20~-15℃, maintained for 2h; and desorption drying temperature is 20~35℃, maintained for 4h, to obtain the final product.

[0043] Comparative Example 4 Prescription components and dosages: 25 parts by weight of Malva nut, 70 parts by weight of Adenophora stricta, 120 parts by weight of stir-fried Cassia seed, 30 parts by weight of Terminalia chebula, 50 parts by weight of Lonicera japonica, 30 parts by weight of Ophiopogon japonicus, and 5 parts by weight of erythritol.

[0044] Preparation method: A. Grind the prescribed amounts of the Chinese medicinal herbs, including Malva nut, honeysuckle, Adenophora stricta, and stir-fried Cassia tora, into powder to 40-60 mesh; remove the pit from the prescribed amount of Terminalia chebula and grind it into powder to 60-80 mesh; grind the prescribed amount of Ophiopogon japonicus into powder to 40-60 mesh; then place each component separately in a decoction pot, add 12 times the amount of water, and decoct twice. Each time, after boiling, boil over high heat for 30 minutes and then simmer over low heat for 2 hours. Combine the decoctions and filter to obtain the extract. B. Take the extracts of the aforementioned components, add them to 30% ethanol (by volume), stir, add the lyophilization excipient, filter through a 0.22μm microporous membrane for sterilization, dispense into vials, and freeze-dry. The steps are as follows: pre-freezing temperature is -40~-45℃, maintained for 3h; sublimation drying temperature is -20~-15℃, maintained for 2h; and desorption drying temperature is 20~35℃, maintained for 4h, to obtain the final product.

[0045] Comparative Example 5 Prescription components and dosage: 120 parts of cassia seed, 25 parts of malva nut, 30 parts of green plum, 50 parts of honeysuckle, 30 parts of ophiopogon japonicus, and 5 parts by weight of erythritol.

[0046] Its preparation method includes the following steps: Step 1: Raw material preparation 1) Take cassia seeds, place them in a stir-frying container, heat over medium heat, stir-fry until the color deepens, they swell slightly, the cross-section is light yellow, and a fragrance is released. Remove and let cool, then grind them into powder. Add 10 times the amount of water to the cassia seed powder, bring to a boil over high heat, then simmer over low heat for about 1 hour, and filter out the filtrate. Add 5 times the amount of water to the residue, bring to a boil over high heat, then simmer over low heat for about half an hour, and collect the filtrate again. Mix the two filtrates, concentrate under reduced pressure to obtain cassia seed extract, and dry for later use. 2) Take honeysuckle, place it in a stir-frying container, heat over low heat, stir-fry until light golden yellow and fragrant, remove and let cool, then crush; place honeysuckle in 15 times the amount of boiling water, heat and decoct for about half an hour, filter out the filtrate; add the residue to 10 times the amount of boiling water, heat and decoct for about 15 minutes, collect the filtrate again; mix the two filtrates, concentrate under reduced pressure to obtain honeysuckle extract, dry for later use; 3) Take the Malva nut, crush it into granules, add about 0.01 kg of a compound enzyme composed of hemicellulase and pectinase (ratio about 1:2) to the granules, maintain the temperature at 30~35℃ and the pH value at 6.5, and carry out enzymatic hydrolysis; add 15 times the amount of 75% ethanol to the enzymatically hydrolyzed Malva nut, soak for about 1 hour, and take the soaking liquid; repeat three times, mix the three soaking liquids, concentrate under reduced pressure to obtain Malva nut extract, and dry for later use; 4) Take Ophiopogon japonicus, crush it into granules, add about 0.015 by weight of a complex enzyme composed of hemicellulase and pectinase (ratio about 1:1) to the granules, maintain the temperature at 30~35℃ and the pH value at 6.0, and carry out enzymatic hydrolysis; add 20 times the amount of 75% ethanol to the hydrolyzed Ophiopogon japonicus, heat under reflux for about half an hour, and filter; add 10 times the amount of 75% ethanol to the filter residue, heat under reflux for half an hour, and filter; mix the two filtrates, concentrate under reduced pressure to obtain Ophiopogon japonicus extract, and dry for later use; 5) Remove the pits from the green plums and crush them. Add 40 times the amount of 65% ethanol aqueous solution and heat to 50~60℃ for about 2 hours. Filter to obtain the extract. Concentrate the extract under reduced pressure to obtain green plum extract and dry it for later use.

[0047] 6) Take the extracts of the above components, add them to 30% ethanol (by volume), stir, add the lyophilization excipient, filter through a 0.22μm microporous membrane for sterilization, dispense into vials, and freeze-dry. The steps are as follows: pre-freezing temperature is -40~-45℃, maintained for 3h; sublimation drying temperature is -20~-15℃, maintained for 2h; and desorption drying temperature is 20~35℃, maintained for 4h.

[0048] Verification Implementation Examples Experimental Example 1: Stability Study of Lyophilized Powder The products obtained from each embodiment and comparative embodiment were placed in an environment of 25±2℃ and 65±5% relative humidity for stability testing. The test results are shown in the table below.

[0049] Table 1 Stability Results The results showed that the freeze-dried powder products prepared in the embodiments of the present invention have excellent long-term stability. After 12 months of storage, their appearance, reconstitution time, taste, pH value, and the content of effective components such as cassia seed extract and ginsenoside D remained basically unchanged compared with the initial state, and the quality remained stable. In Comparative Example 1, because ginseng was not added, the product still had a noticeable bitter taste even with the addition of erythritol for flavoring, resulting in a poor taste. In Comparative Example 2, the excipient was replaced with mannitol, which led to poor reconstitution and prolonged reconstitution time in the product. In Comparative Example 3, the extraction process did not use ultrasound-assisted alcohol extraction or water extraction, resulting in a low extraction rate and decreased content of effective components such as cassia seed extract and ginsenoside D in the product. Comparative Example 4 used a simple water extraction method to obtain effective components, resulting in poor product quality and a significant decrease in the content of effective components. Comparative Example 5 used other extraction methods, resulting in unstable product quality, not only with a bitter taste but also a significant decrease in the content of effective components.

[0050] Experiment Example 2: Investigation of the effect of voice clearing and lung moisturizing on patients with pharyngitis Fifty subjects with typical pharyngitis symptoms were selected. Inclusion criteria included clinical manifestations such as dry mouth and tongue, a foreign body sensation in the throat, hoarseness, vocal fatigue, and sore throat, with symptoms lasting for more than six months, and a clinical diagnosis of chronic pharyngitis. Experimental method: 5g of the product prepared in Example 1 of this invention was taken three times daily with warm water for 30 consecutive days. Efficacy was evaluated based on the traditional Chinese medicine symptom and sign scoring criteria.

[0051] Significant effect: Clinical symptoms are significantly reduced or completely disappeared, pharyngeal signs are reduced by 2 grades, and the individual symptom score is reduced by ≥4 points; Effective: Clinical symptoms are alleviated, pharyngeal signs improve by 1-2 grades, and individual symptom scores decrease by 2-3 points; Ineffective: No significant changes in clinical symptoms and signs, and a decrease of <2 points in the score of a single symptom item.

[0052] The results showed that after taking the instant lung-clearing and throat-moistening freeze-dried powder of the present invention for 30 days, most subjects experienced significant relief from symptoms such as dry mouth and throat, foreign body sensation in the throat, and hoarseness.

Claims

1. A fast-dissolving, lung-moistening, and refreshing freeze-dried tea powder, characterized in that... The freeze-dried powder contains Malva nut, Adenophora stricta, stir-fried Cassia seed, Terminalia chebula, honeysuckle, Ophiopogon japonicus and freeze-drying excipients. The components are mixed and then freeze-dried to obtain the freeze-dried powder.

2. The freeze-dried powder as described in claim 1, characterized in that, In the freeze-dried powder, the freeze-drying excipient is selected from any one or more of erythritol, allulose, and steviol glycosides.

3. The freeze-dried powder as described in claim 2, characterized in that, The freeze-dried powder contains 5 to 20 parts by weight of freeze-drying excipient.

4. The freeze-dried powder according to claim 1, characterized in that, In the freeze-dried powder, the weight parts of Malva nut are 25-90 parts, the weight parts of Adenophora stricta are 70-100 parts, the weight parts of stir-fried Cassia tora seeds are 120-190 parts, the weight parts of Terminalia chebula are 30-70 parts, the weight parts of Lonicera japonica are 50-110 parts, and the weight parts of Ophiopogon japonicus are 30-110 parts.

5. A method for preparing the freeze-dried powder as described in claim 1, characterized in that, The method includes the following steps: A. Pulverize the Chinese medicinal herbs Malva nut, honeysuckle, Adenophora stricta, and stir-fried cassia seed separately; add the pulverized Malva nut to 20-30% ethanol (v / v), and perform ultrasound-assisted extraction at room temperature. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Malva nut extract; add the pulverized honeysuckle to 60-75% ethanol (v / v), and perform ultrasound-assisted heating extraction. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain honeysuckle extract; add the pulverized Adenophora stricta to purified water, and perform ultrasound-assisted extraction at low temperature. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Adenophora stricta extract; add the pulverized stir-fried cassia seed to 40-55% ethanol (v / v), and perform ultrasound-assisted extraction with simultaneous heating. After extraction, centrifuge, collect the supernatant, and concentrate under reduced pressure to obtain Adenophora stricta extract. B. After removing the pits from the green plums, crush them and then extract them with 40-60% ethanol by volume. The extract is dried with infrared radiation-assisted drying while being stirred to obtain green plum extract. C. After pulverizing and vacuum drying, the Ophiopogon japonicus was separated and extracted by countercurrent chromatography, and concentrated by nanofiltration to obtain the enriched Ophiopogon japonicus components; D. Take the extracts of the above components, add them to ethanol with a volume fraction of 30-40%, stir, add lyophilization excipients, filter through a 0.22μm microporous membrane for sterilization, dispense into vials, and freeze-dry to obtain instant-dissolving Qingyin Runfei Tea lyophilized powder.

6. The preparation method according to claim 5, characterized in that, In the method, during step A, when extracting Malva nut, the ultrasonic frequency is 25~30kHz and the time is 10~20min.

7. The preparation method according to claim 5, characterized in that, In the method, during step A, when extracting honeysuckle, the ultrasonic frequency is 20~25kHz, the temperature is 40~45℃, and the time is 30~50min.

8. The preparation method according to claim 5, characterized in that, In the method, during step A, when extracting Adenophora stricta, the ultrasonic frequency is 30~35kHz, the temperature is 10~20℃, and the time is 30~40min.

9. The preparation method according to claim 5, characterized in that, In the method, during step A, when extracting roasted cassia seeds, the ultrasonic frequency is 35~40kHz, the temperature is 55~60℃, and the time is 40~60min.

10. The preparation method according to claim 5, characterized in that, In the method described, step D, the freeze-drying process is as follows: the pre-freezing temperature is -40~-45℃, maintained for 3~4 hours; the sublimation drying temperature is -20~-15℃, maintained for 2~3 hours; and the desorption drying temperature is 20~35℃, maintained for 4~6 hours.

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