A centrifuge container and separation method based on gradient centrifugation

Abstract

This invention discloses a centrifuge container and separation method based on gradient centrifugation, relating to the field of centrifuge container technology. It includes a lower cover and an active concentricity correction component. An upper cover is fixed to the top of the lower cover, and the upper flow channel is connected to the upper flow channel interface. The active concentricity correction component is disposed on the top of the inner wall of the mounting base. This gradient centrifugation-based centrifuge container and separation method constructs an independent closed-loop flow channel system, enabling uninterrupted operation of continuous sample feeding, cell gradient centrifugation, and simultaneous waste discharge, completely solving the problems of low efficiency and cumbersome processes in traditional intermittent centrifugation. Simultaneously, relying on the high-transparency viewing window built into the lower cover, combined with a microscopic imaging system and color camera arranged on both sides of the servo motor output shaft, real-time or periodic visual observation can be achieved throughout the entire process of centrifugation, washing and purification, and cell resuspension, accurately grasping the cell enrichment state and separation effect, and effectively avoiding problems such as cell damage, incomplete separation, and insufficient purification.

Description

Technical Field

[0001] This invention relates to the field of centrifuge container technology, specifically to a centrifuge container and separation method based on gradient centrifugation. Background Technology

[0002] Gradient centrifugation is a core method for the separation and purification of biological samples. It is widely used for the fractional separation of samples such as cells, microorganisms, and biomolecules. Currently, most centrifuge containers used in gradient centrifugation adopt an intermittent separation mode, which cannot achieve continuous sample liquid inflow and simultaneous waste liquid outflow, resulting in low separation efficiency and difficulty in meeting the needs of continuous processing of large batches of samples.

[0003] Moreover, the concentricity of the centrifuge cup and the drive shaft depends only on the initial assembly accuracy. After long-term use, concentricity deviations are easily caused by component wear, aging, and loose assembly. This can lead to severe vibrations during high-speed rotation, which reduces separation accuracy and can easily damage the equipment. Furthermore, the concentricity can only be calibrated periodically and cannot be adjusted in real time. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a centrifuge container and separation method based on gradient centrifugation, which solves the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a centrifuge container based on gradient centrifugation, comprising a lower cover and an active concentricity correction component. An upper cover is fixed to the top of the lower cover, and a sealing element is embedded in the center of the top surface of the upper cover. An interface tube is inserted through and rotatably connected to the inside of the sealing element. An upper flow channel interface and a lower flow channel interface are sequentially arranged on the outer wall of the interface tube from bottom to top. A lower flow channel opening is provided at the bottom of the inner wall of the lower cover, and the bottom of the lower flow channel opening is connected to a lower flow channel. An upper flow channel opening is embedded in the inner wall of the upper cover, and the top of the upper flow channel opening is connected to an upper flow channel. The lower flow channel is connected to the lower flow channel interface, and the upper flow channel is connected to the upper flow channel interface. A mounting base is provided at the bottom of the lower cover, and the output shaft of a servo motor is inserted into the inside of the mounting base. The active concentricity correction component is located on the top of the inner wall of the mounting base.

[0006] Furthermore, a highly transparent viewing window is provided at the bottom of the lower cover, and a microscopic imaging system and a color camera are respectively provided on both sides of the servo motor output shaft.

[0007] Furthermore, the concentricity active correction component includes a concentricity detection sensor, and a correction box is provided at the bottom of the concentricity detection sensor.

[0008] Furthermore, the correction box has a ring-shaped track cavity inside, and an electromagnetic plate is fixed at the end of the track cavity. The track cavity is equipped with movable ball bearings.

[0009] Furthermore, a dielectric cavity is provided on the side of the track cavity away from the electromagnetic plate, and an elastic transmission rod passes through the interior of the dielectric cavity.

[0010] Furthermore, the end of the medium cavity is connected to an electromagnetic flow valve via a medium channel, and the bottom of the electromagnetic flow valve is connected to an output cavity via a medium channel.

[0011] Furthermore, the output cavity is provided with elastic correction plates, and at least three elastic correction plates are provided.

[0012] Furthermore, the elastic correction piece is arranged one-to-one with the track cavity, and the elastic correction piece is arranged in a ring shape, and the elastic correction piece is distributed on the side of the end of the servo motor output shaft.

[0013] A separation method based on gradient centrifugation, which utilizes the aforementioned gradient centrifugation centrifuge container, includes the following steps:

[0014] S1: The upper flow channel interface is used for the supply of sample solution and washing solution, and the lower flow channel interface is used for the discharge of waste liquid. The pump speed is adjusted to ensure that the sample inflow rate matches the waste outflow rate.

[0015] S2: Start the servo motor to drive the cup body consisting of the lower cover and the upper cover to rotate, and start the upper flow channel sample solution supply pump so that the cell sample containing culture medium is pumped into the cup body through the upper flow channel opening;

[0016] S3: Under centrifugation, the cells are located on the outside and the culture medium is located on the inside. The waste liquid discharge pump in the downflow channel is started so that the culture medium on the inside is pumped out synchronously through the downflow channel, realizing continuous operation of sample entry and waste liquid exit.

[0017] S4: In the newly entered sample, the cells are thrown against the cup wall and the culture medium is discharged through the lower outlet. After the sample loading is completed, the supply to the upper outlet is switched to washing solution, and the washing solution is continuously introduced to rinse the cells and further remove residual culture medium or impurities.

[0018] S5: Observe the cell status in real time or periodically using a microscopic imaging system and color camera. During the cell collection and final stage, stop the supply of sample solution and continue to flush the tubing with washing solution to ensure that the waste liquid in the cup is drained. Reduce the centrifugation speed and pump in cell preservation solution or culture medium through the upper flow channel interface to resuspend the cells on the cup wall. Then collect the concentrated cell suspension through the lower or upper flow channel.

[0019] S6: When the servo motor drives the cup to rotate, the concentricity detection sensor monitors the concentricity data between the cup and the output shaft of the servo motor in real time, and controls the opening and closing degree and the cut-off time of each electromagnetic flow valve based on the concentricity deviation.

[0020] S7: Based on centrifugal force, the moving ball pushes the elastic transmission rod, which in turn presses into the interior of the medium cavity, causing the medium to enter the output cavity along the medium channel and the electromagnetic flow valve. This pushes the elastic correction plate to extend. The extension distance of each elastic correction plate is controlled by the flow rate and time of the electromagnetic flow valve. When the elastic correction plate extends to the point where the concentricity detection meets the preset value, the electromagnetic flow valve closes, thereby locking the extension distance of the elastic correction plate and preventing it from extending or retracting arbitrarily. Thus, the concentricity is adjusted by the adaptive extension of each elastic correction plate, thereby reducing the concentricity deviation in real time.

[0021] S8: When adjusting the extension of each elastic correction piece in real time, it is necessary to retract the elastic correction piece to better coordinate and adjust the concentricity. When the elastic correction piece needs to retract, the opening degree of the electromagnetic flow valve must be adjusted first, and then the electromagnetic plate is energized to attract the moving ball. At this time, the moving ball is freed from the influence of centrifugal force and is attracted to the position of the electromagnetic plate, so that the elastic transmission rod and the elastic correction piece rebound and retract under the action of the spring until the elastic correction piece retracts to the preset distance, and then the electromagnetic flow valve is closed to lock.

[0022] This invention provides a centrifuge container and separation method based on gradient centrifugation, which has the following beneficial effects:

[0023] 1. This gradient centrifugation-based centrifugation container and separation method constructs an independent closed-loop flow channel system, stably achieving the directional supply of sample solution, washing solution, and cell preservation solution, while rapidly completing the synchronous discharge of waste liquid and impurities. This enables uninterrupted operation of continuous sample feeding, gradient cell centrifugation, and synchronous waste liquid discharge, completely solving the problems of low efficiency and cumbersome process of traditional intermittent centrifugation. At the same time, relying on the high-transparency viewing window built into the lower cover, combined with the microscopic imaging system and color camera deployed on both sides of the servo motor output shaft, real-time or periodic visualization observation can be achieved throughout the entire process of centrifugation, washing and purification, and cell resuspension. This allows for precise control of cell enrichment status and separation effect, effectively avoiding problems such as cell damage, incomplete separation, and insufficient purification, and significantly improving the stability, accuracy, and sample recovery rate of gradient centrifugation.

[0024] 2. This gradient centrifuge-based centrifuge container and separation method uses a concentricity detection sensor to monitor the concentricity deviation between the container and the servo motor output shaft in real time. Combined with the coordinated operation of the track cavity, electromagnetic plate, movable ball bearings, elastic transmission rod, media cavity, electromagnetic flow valve, and elastic correction plate within the correction box, the elastic correction plate can adaptively extend by relying on centrifugal force to drive media transmission. The electromagnetic plate attracts the movable ball bearings, and the electromagnetic flow valve precisely controls the retraction and positioning locking of the elastic correction plate. This real-time dynamic correction of concentricity deviations caused by assembly, wear, and aging significantly reduces vibration and eccentricity losses during high-speed centrifugation, substantially improving centrifugal operation stability and separation accuracy, while effectively extending the overall service life of the equipment. Attached Figure Description

[0025] Figure 1 This is a three-dimensional structural diagram of a centrifuge container based on gradient centrifugation according to the present invention;

[0026] Figure 2 This is a schematic cross-sectional view of the upper and lower covers of a centrifuge container based on gradient centrifugation according to the present invention.

[0027] Figure 3 This is a schematic cross-sectional view of the correction box structure of a centrifuge container based on gradient centrifugation according to the present invention.

[0028] Figure 4 This is a top view schematic diagram of the track cavity structure of a centrifuge container based on gradient centrifugation according to the present invention;

[0029] Figure 5 This is a schematic diagram of the steps of a separation method based on gradient centrifugation according to the present invention.

[0030] In the diagram: 1. Lower cover; 2. Upper cover; 3. Seal; 4. Interface pipe; 5. Upper flow channel interface; 6. Lower flow channel interface; 7. Lower flow channel opening; 8. Lower flow channel; 9. Upper flow channel opening; 10. Upper flow channel; 11. Mounting base; 12. Servo motor; 13. Concentricity active correction component; 1301. Concentricity detection sensor; 1302. Correction box; 1303. Track cavity; 1304. Electromagnetic plate; 1305. Moving ball; 1306. Medium cavity; 1307. Elastic transmission rod; 1308. Electromagnetic flow valve; 1309. Output cavity; 1310. Elastic correction plate; 14. Microscopic imaging system; 15. Color camera. Detailed Implementation

[0031] The embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of the invention.

[0032] like Figures 1-5As shown, the present invention provides a technical solution: a centrifuge container based on gradient centrifugation, including a lower cover 1 and a concentricity active correction component 13. An upper cover 2 is fixed to the top of the lower cover 1, and a sealing element 3 is embedded in the center of the top surface of the upper cover 2. An interface tube 4 is inserted through and rotatably connected to the inside of the sealing element 3. An upper flow channel interface 5 and a lower flow channel interface 6 are sequentially arranged on the outer wall of the interface tube 4 from bottom to top. A lower flow channel opening 7 is opened at the bottom of the inner wall of the lower cover 1, and the bottom of the lower flow channel opening 7 is connected to a lower flow channel 8. An upper flow channel interface 5 and a lower flow channel interface 6 are embedded in the inner wall of the upper cover 2. The upper flow channel 9 is connected to the top of the upper flow channel 9 via the upper flow channel 10. The lower flow channel 8 is connected to the lower flow channel interface 6, and the upper flow channel 10 is connected to the upper flow channel interface 5. The bottom of the lower cover 1 is provided with a mounting base 11, and the output shaft of the servo motor 12 is inserted into the interior of the mounting base 11. The concentricity active correction component 13 is located on the top of the inner wall of the mounting base 11. The bottom of the lower cover 1 is provided with a high-transparency viewing window. The two sides of the output shaft of the servo motor 12 are respectively provided with a microscopic imaging system 14 and a color camera 15.

[0033] A gradient centrifugation-based separation method, which utilizes the aforementioned gradient centrifugation-based centrifuge container, includes the following steps:

[0034] S1: Upper flow channel interface 5 is used for sample liquid and washing liquid supply, and lower flow channel interface 6 is used for waste liquid discharge. The pump speed is adjusted to ensure that the sample liquid inflow rate and the waste liquid outflow rate are matched.

[0035] S2: Start the servo motor 12 to drive the cup body composed of the lower cover 1 and the upper cover 2 to rotate, and start the upper flow channel sample solution supply pump so that the cell sample containing culture medium is pumped into the cup body through the upper flow channel port 9.

[0036] S3: Under centrifugation, the cells are located on the outside and the culture medium is located on the inside. The waste liquid discharge pump in the downflow channel is started so that the culture medium on the inside is pumped out through the downflow channel port 7 simultaneously, realizing continuous operation of sample entry and waste liquid exit.

[0037] S4: In the newly entered sample, the cells are thrown to the cup wall and the culture medium is discharged through the lower outlet 7. After the sample loading is completed, the upper outlet 5 is switched to the washing solution, and the washing solution is continuously introduced to rinse the cells and further remove residual culture medium or impurities.

[0038] S5: The cell status is observed in real time or periodically through the microscopic imaging system 14 and the color camera 15. During the cell collection and end stages, the supply of sample solution is stopped, and only the washing solution is continued to flush the tubing to ensure that the waste liquid in the cup is drained. The centrifugation speed is reduced, and cell preservation solution or culture medium is pumped in through the upper flow channel interface 5 to resuspend the cells on the cup wall. The concentrated cell suspension is then collected through the lower or upper flow channel.

[0039] Based on the above description, the present invention constructs an independent closed-loop flow channel system through a lower cover 1, an upper cover 2, a sealing element 3, an interface tube 4, an upper flow channel interface 5, a lower flow channel interface 6, a lower flow channel opening 7, a lower flow channel 8, an upper flow channel opening 9, and an upper flow channel 10. The upper flow channel interface 5 is connected to the upper flow channel opening 9 via the upper flow channel 10, enabling a stable and directional supply of sample solution, washing solution, and cell preservation solution. The lower flow channel interface 6 is connected to the lower flow channel opening 7 via the lower flow channel 8, allowing for the rapid and simultaneous discharge of waste liquid and impurities. This is further enhanced by a servo motor 12 driving the cup body to rotate at high speed, and precise adjustment of the pump speed to control the inflow and outflow rates. Dynamic matching of quantities enables uninterrupted operation of continuous sample loading, cell gradient centrifugation, and simultaneous waste discharge, completely solving the problems of low efficiency and cumbersome process of traditional intermittent centrifugation. At the same time, relying on the high-transparency viewing window of the lower cover 1, and with the microscopic imaging system 14 and color camera 15 arranged on both sides of the output shaft of the servo motor 12, real-time or periodic visualization observation can be achieved throughout the entire process of centrifugation, washing and purification, and cell resuspension. This allows for precise control of cell enrichment status and separation effect, effectively avoiding problems such as cell damage, incomplete separation, and insufficient purification, and significantly improving the stability, accuracy, and sample recovery rate of gradient centrifugation.

[0040] like Figures 1-5 As shown, the concentricity active correction component 13 includes a concentricity detection sensor 1301, and a correction box 1302 is provided at the bottom of the concentricity detection sensor 1301. The correction box 1302 has a ring-shaped track cavity 1303 inside, and an electromagnetic plate 1304 is fixed at one end of the track cavity 1303. A movable ball bearing 1305 is provided inside the track cavity 1303. A dielectric cavity 1306 is provided on the side of the track cavity 1303 away from the electromagnetic plate 1304, and an elastic transmission rod 1 passes through the dielectric cavity 1306. 307. The end of the medium cavity 1306 is connected to the electromagnetic flow valve 1308 through the medium channel, and the bottom of the electromagnetic flow valve 1308 is connected to the output cavity 1309 through the medium channel. An elastic correction plate 1310 is provided inside the output cavity 1309, and at least three elastic correction plates 1310 are provided. The elastic correction plates 1310 are arranged one-to-one with the track cavity 1303, and the elastic correction plates 1310 are arranged in a ring shape. The elastic correction plates 1310 are distributed on the side of the end of the output shaft of the servo motor 12.

[0041] The specific operation is as follows: A flexible rubber ring is provided on the inner wall of the mounting base 11. The flexible rubber ring makes the inner wall of the mounting base 11 and the outer wall of the output shaft end of the servo motor 12 have an adjustable gap. When the servo motor 12 drives the cup to rotate, the concentricity detection sensor 1301 monitors the concentricity data of the cup and the output shaft of the servo motor 12 in real time. Based on the concentricity deviation, the opening degree and closing time of each electromagnetic flow valve 1308 are controlled.

[0042] Simultaneously, the centrifugal force generated by high-speed rotation causes the movable ball bearing 1305 to push the elastic transmission rod 1307, thus pressing the elastic transmission rod 1307 into the interior of the medium chamber 1306, allowing the medium to enter the output chamber 1309 along the medium channel and the electromagnetic flow valve 1308. This pushes the elastic correction plates 1310 to extend. The extension distance of each elastic correction plate 1310 is controlled by the flow rate and time of the electromagnetic flow valve 1308. When the elastic correction plate 1310 extends to the point where the concentricity detection meets the preset value, the electromagnetic flow valve 1308 closes, thereby locking the extension distance of the elastic correction plate 1310 so that it can no longer extend or retract arbitrarily. Thus, the concentricity is adjusted by the adaptive extension of each elastic correction plate 1310, thereby reducing the concentricity deviation in real time and avoiding excessive concentricity deviation due to wear, aging, etc. during long-term use, which would affect the centrifugation effect.

[0043] When adjusting the extension amount of each elastic correction piece 1310 in real time, in order to better coordinate and adjust the concentricity, the elastic correction piece 1310 needs to retract. When the elastic correction piece 1310 needs to retract, the opening degree of the electromagnetic flow valve 1308 needs to be adjusted first, and then the electromagnetic piece 1304 is energized to attract the movable ball 1305. At this time, the movable ball 1305 is freed from the influence of centrifugal force and is attracted to the position of the electromagnetic piece 1304, so that the elastic transmission rod 1307 and the elastic correction piece 1310 rebound and retract under the action of the spring until the elastic correction piece 1310 retracts to the preset distance, and then the electromagnetic flow valve 1308 closes to lock.

[0044] Based on the above description, this invention uses a concentricity detection sensor 1301 to monitor the concentricity deviation between the cup body and the output shaft of the servo motor 12 in real time. In conjunction with the coordinated operation of the track cavity 1303, electromagnetic plate 1304, movable ball bearing 1305, elastic transmission rod 1307, medium cavity 1306, electromagnetic flow valve 1308, and elastic correction plate 1310 within the correction box 1302, the elastic correction plate 1310 can adaptively extend by relying on centrifugal force to drive the medium transmission. Furthermore, the electromagnetic plate 1304 adsorbs the movable ball bearing 1305, and the electromagnetic flow valve 1308 precisely controls the retraction and positioning locking of the elastic correction plate 1310. This provides real-time dynamic correction of concentricity deviations caused by assembly, wear, and aging, significantly reducing vibration and eccentricity losses during high-speed centrifugation, significantly improving centrifugal operation stability and separation accuracy, and effectively extending the overall service life of the equipment.

[0045] In summary, this gradient centrifugation-based centrifugation container and separation method, during use, uses the upper flow channel interface 5 for supplying sample solution and washing solution, and the lower flow channel interface 6 for discharging waste liquid. The pump speed is adjusted to ensure that the sample inflow and waste outflow are matched. The servo motor 12 is started to drive the cup body composed of the lower cover 1 and the upper cover 2 to rotate, and the upper flow channel sample solution supply pump is started, so that the cell sample containing culture medium is pumped into the cup body through the upper flow channel port 9. Under centrifugation, the cells are located on the outside and the culture medium is located on the inside. The lower flow channel waste liquid discharge pump is started to pump the culture medium on the inside out through the lower flow channel port 7 simultaneously, realizing continuous operation of sample inflow and waste liquid outflow.

[0046] In the newly arrived sample, cells are thrown against the cup wall, and the culture medium is discharged through the lower outlet 7. After the sample loading is completed, the supply of the upper outlet 5 is switched to washing solution, and the washing solution is continuously circulated to rinse the cells and further remove residual culture medium or impurities. The cell status is observed in real time or periodically through the microscopic imaging system 14 and the color camera 15. During the cell collection and final stage, the supply of sample solution is stopped, and only the washing solution is continuously circulated to rinse the tubing to ensure that the waste liquid in the cup is completely drained. The centrifugation speed is reduced, and cell preservation solution or culture medium is pumped in through the upper outlet 5 to resuspend the cells on the cup wall. The concentrated cell suspension is then collected through the lower or upper outlet.

[0047] The inner wall of the mounting base 11 is provided with a flexible rubber ring. The flexible rubber ring allows for an adjustable gap between the inner wall of the mounting base 11 and the outer wall of the output shaft end of the servo motor 12. When the servo motor 12 drives the cup to rotate, the concentricity detection sensor 1301 monitors the concentricity data between the cup and the output shaft of the servo motor 12 in real time, and controls the opening and closing degree and the closing time of each electromagnetic flow valve 1308 based on the concentricity deviation.

[0048] Simultaneously, the centrifugal force generated by high-speed rotation causes the movable ball bearing 1305 to push the elastic transmission rod 1307, thus pressing the elastic transmission rod 1307 into the interior of the medium chamber 1306, allowing the medium to enter the output chamber 1309 along the medium channel and the electromagnetic flow valve 1308. This pushes the elastic correction plates 1310 to extend. The extension distance of each elastic correction plate 1310 is controlled by the flow rate and time of the electromagnetic flow valve 1308. When the elastic correction plate 1310 extends to the point where the concentricity detection meets the preset value, the electromagnetic flow valve 1308 closes, thereby locking the extension distance of the elastic correction plate 1310 so that it can no longer extend or retract arbitrarily. Thus, the concentricity is adjusted by the adaptive extension of each elastic correction plate 1310, thereby reducing the concentricity deviation in real time and avoiding excessive concentricity deviation due to wear, aging, etc. during long-term use, which would affect the centrifugation effect.

[0049] When adjusting the extension amount of each elastic correction piece 1310 in real time, it is necessary to retract the elastic correction piece 1310 to better coordinate and adjust the concentricity. When the elastic correction piece 1310 needs to retract, the opening degree of the electromagnetic flow valve 1308 must be adjusted first, and then the electromagnetic plate 1304 is energized to attract the movable ball 1305. At this time, the movable ball 1305 is freed from the influence of centrifugal force and is attracted to the position of the electromagnetic plate 1304, so that the elastic transmission rod 1307 and the elastic correction piece 1310 rebound and retract under the action of the spring until the elastic correction piece 1310 retracts to the preset distance, and then the electromagnetic flow valve 1308 closes to lock.

[0050] The embodiments of the present invention are given for illustrative and descriptive purposes only, and are not intended to be exhaustive or to limit the invention to the forms disclosed. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described in order to better illustrate the principles and practical application of the invention, and to enable those skilled in the art to understand the invention and to design various embodiments with various modifications suitable for a particular purpose.

Claims

1. A centrifuge container based on gradient centrifugation, comprising a lower cover (1) and a concentricity active correction component (13), characterized in that: The top of the lower cover (1) is fixed with an upper cover (2), and a sealing element (3) is embedded in the middle of the top surface of the upper cover (2). An interface tube (4) is inserted and rotatably connected inside the sealing element (3), and the outer wall of the interface tube (4) is provided with an upper flow channel interface (5) and a lower flow channel interface (6) from bottom to top. A lower flow channel opening (7) is opened at the bottom of the inner wall of the lower cover (1), and the bottom of the lower flow channel opening (7) is connected to a lower flow channel (8). The inner wall of the upper cover (2) is embedded with an upper flow channel interface (5) and a lower flow channel interface (6). The upper flow channel (9) is connected to the top of the upper flow channel (9) and the upper flow channel (10). The lower flow channel (8) is connected to the lower flow interface (6), and the upper flow channel (10) is connected to the upper flow interface (5). The bottom of the lower cover (1) is provided with a mounting base (11), and the output shaft of the servo motor (12) is inserted into the inside of the mounting base (11). The concentricity active correction component (13) is located on the top of the inner wall of the mounting base (11).

2. A centrifuge container based on gradient centrifugation according to claim 1, characterized in that: The bottom of the lower cover (1) is provided with a highly transparent viewing window, and a microscopic imaging system (14) and a color camera (15) are respectively provided on both sides of the output shaft of the servo motor (12).

3. A centrifuge container based on gradient centrifugation according to claim 1, characterized in that: The concentricity active correction component (13) includes a concentricity detection sensor (1301), and a correction box (1302) is provided at the bottom of the concentricity detection sensor (1301).

4. A centrifuge container based on gradient centrifugation according to claim 3, characterized in that: The correction box (1302) has a ring-shaped track cavity (1303) inside, and an electromagnetic plate (1304) is fixed at the end of the track cavity (1303). A movable ball (1305) is provided inside the track cavity (1303).

5. A centrifuge container based on gradient centrifugation according to claim 4, characterized in that: A medium cavity (1306) is provided on the side of the track cavity (1303) away from the electromagnetic plate (1304), and an elastic transmission rod (1307) is inserted inside the medium cavity (1306).

6. A centrifuge container based on gradient centrifugation according to claim 5, characterized in that: The end of the medium chamber (1306) is connected to an electromagnetic flow valve (1308) through a medium channel, and the bottom of the electromagnetic flow valve (1308) is connected to an output chamber (1309) through a medium channel.

7. A centrifuge container based on gradient centrifugation according to claim 6, characterized in that: The output cavity (1309) is provided with an elastic correction piece (1310), and at least three elastic correction pieces (1310) are provided.

8. A centrifuge container based on gradient centrifugation according to claim 7, characterized in that: The elastic correction piece (1310) is arranged one-to-one with the track cavity (1303), and the elastic correction piece (1310) is arranged in a ring shape. Moreover, the elastic correction piece (1310) is distributed on the side of the end of the output shaft of the servo motor (12).

9. A separation method based on gradient centrifugation, wherein the centrifuge container based on gradient centrifugation as described in any one of claims 1-8 is characterized in that: The gradient centrifugation-based separation method includes the following steps: S1: The upper flow channel interface (5) is used for the supply of sample liquid and cleaning liquid, and the lower flow channel interface (6) is used for the discharge of waste liquid. The pump speed is adjusted to ensure that the sample inflow and waste outflow are matched. S2: Start the servo motor (12) to drive the cup body composed of the lower cover (1) and the upper cover (2) to rotate, start the upper flow channel sample liquid supply pump, so that the cell sample containing culture medium is pumped into the cup body through the upper flow channel port (9); S3: Under centrifugation, the cells are located on the outside and the culture medium is located on the inside. Start the waste liquid discharge pump in the downflow channel so that the culture medium on the inside is pumped out through the downflow channel (7) simultaneously, so as to realize the continuous operation of sample entry and waste liquid exit. S4: In the newly entered sample, the cells are thrown to the cup wall and the culture medium is discharged through the lower flow channel (7). After the sample loading is completed, the upper flow channel interface (5) is switched to the washing solution, and the washing solution is continuously introduced to rinse the cells and further remove residual culture medium or impurities. S5: Observe the cell status in real time or periodically through the microscopic imaging system (14) and color camera (15). During the cell collection and end stage, stop the supply of sample solution and only continue to flush the tubing with washing solution to ensure that the waste liquid in the cup is drained. Reduce the centrifugation speed and pump in cell preservation solution or culture medium through the upper flow channel interface (5) to resuspend the cells on the cup wall. Then collect the concentrated cell suspension through the lower or upper flow channel. S6: When the servo motor (12) drives the cup to rotate, the concentricity detection sensor (1301) monitors the concentricity data of the cup and the output shaft of the servo motor (12) in real time, and controls the opening and closing degree and the closing time of each electromagnetic flow valve (1308) based on the concentricity deviation. S7: Based on centrifugal force, the movable ball (1305) pushes the elastic transmission rod (1307), and the elastic transmission rod (1307) is thus pressed into the interior of the medium cavity (1306), so that the medium enters the output cavity (1309) along the medium channel and the electromagnetic flow valve (1308), thereby pushing the elastic correction plate (1310) to extend. The extension distance of each elastic correction plate (1310) is controlled by the flow rate and time of the electromagnetic flow valve (1308), and the electromagnetic flow valve (1308) closes when the elastic correction plate (1310) extends to the point where the concentricity detection meets the preset value, thereby locking the extension distance of the elastic correction plate (1310) so that it can no longer extend or retract arbitrarily. Thus, the concentricity is adjusted by the adaptive extension of each elastic correction plate (1310), thereby reducing the concentricity deviation in real time. S8: When adjusting the extension amount of each elastic correction piece (1310) in real time, in order to better coordinate and adjust the concentricity, the elastic correction piece (1310) needs to retract. When the elastic correction piece (1310) needs to retract, the opening degree of the electromagnetic flow valve (1308) needs to be adjusted first, and then the electromagnetic piece (1304) is powered on to attract the movable ball (1305). At this time, the movable ball (1305) is freed from the influence of centrifugal force and is attracted to the position of the electromagnetic piece (1304), so that the elastic transmission rod (1307) and the elastic correction piece (1310) rebound and retract under the action of the spring until the elastic correction piece (1310) retracts to the preset distance, and then the electromagnetic flow valve (1308) closes to lock.

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