A microdroplet extrusion type bio 3D printing device and a preparation method thereof
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- FOURTH MILITARY MEDICAL UNIVERSITY
- Filing Date
- 2026-05-28
- Publication Date
- 2026-07-21
Smart Images
Figure CN122425894A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of bio-3D printing technology, and in particular to a microdroplet extrusion bio-3D printing device and its preparation method. Background Technology
[0002] Tissue engineering and regenerative medicine are core research areas for solving major medical problems such as tissue defects and organ failure. Bio-3D printing technology, as a core supporting technology in this field, can achieve the customized construction of artificial tissues, wound repair scaffolds, drug screening models, etc. through the precise deposition of cell-loaded bio-ink, providing a new technical path for personalized medicine and precision treatment.
[0003] Microdroplet extrusion printing technology, with its advantages of small droplet size, precise deposition positioning, and low cell damage, has become the mainstream technology for cell-borne printing and has been widely used in fields such as skin wound repair, vascular network construction, and cartilage and bone tissue regeneration. This technology deposits bio-ink carrying living cells in the form of microdroplets on demand, enabling precise control of cell distribution and matrix assembly in three-dimensional space to construct tissue engineering scaffolds with biomimetic structures and functions.
[0004] However, existing traditional bio-3D printing devices and methods typically use a combination of syringe and pneumatic pressure or mechanical push rod to extrude bio-ink during the microdroplet extrusion process. Due to the limited driving control precision of existing devices, problems such as microdroplet adhesion, uneven size, and disordered spacing can easily occur during the printing process, which directly leads to uneven porosity and internal structural defects in the printed scaffold.
[0005] Therefore, there is an urgent need for a microdroplet extrusion bio-3D printing device with more uniform droplet size and spacing. Summary of the Invention
[0006] The purpose of this invention is to provide a microdroplet extrusion-type bio-3D printing device and its preparation method to solve the problems existing in the prior art. By driving the push handle of the syringe through the lead screw assembly, the uniform distribution of shear force during the extrusion process is ensured, thereby improving the uniformity of the size and spacing of the extruded microdroplets.
[0007] To achieve the above objectives, the present invention provides the following solution: This invention provides a microdroplet extrusion-type bio-3D printing device, comprising: a first adjustment component, a second adjustment component, a syringe, and a lead screw assembly; wherein, the first adjustment component is vertically arranged and is used to drive an object to move in a vertical direction; the second adjustment component is horizontally mounted on the first adjustment component and is used to drive the object to move in a horizontal direction; the syringe is mounted on the second adjustment component, and the plunger of the syringe is connected to the lead screw assembly.
[0008] As one embodiment, it also includes a mounting base and an angle adjustment member, the angle adjustment member being mounted on the second adjustment assembly, the mounting base being mounted on the angle adjustment member, the syringe being mounted on the mounting base, and the angle adjustment member being capable of adjusting the angle of the mounting base.
[0009] As one embodiment, it further includes a third adjustment component and a culture plate. The third adjustment component is horizontally arranged, and the adjustment direction of the third adjustment component is perpendicular to the adjustment direction of the second adjustment component. The culture plate is mounted on the third adjustment component. The third adjustment component includes a third slide rod and a third slide block. A third slide groove is formed on the third slide rod, and the third slide block is slidably mounted in the third slide groove. A limit mechanism is provided on the third slide block to detachably mount the culture plate on the third slide block.
[0010] As one embodiment, the limiting mechanism includes a limiting plate and two movable baffles. The limiting plate is fixedly disposed on the upper side of the third slide, and the limiting plate and the third slide are spaced apart. The two movable baffles are spaced apart on the third slide and are rotatably connected to the third slide. The bottom of the culture plate is provided with an outwardly snapping protrusion. The distance between the limiting plate and the third slide matches the thickness of the snapping protrusion. The side wall of the limiting plate is provided with a first abutting surface and a second abutting surface. The end of the first abutting surface away from the limiting plate is inclined towards the second abutting surface, and the end of the second abutting surface away from the limiting plate is inclined towards the first abutting surface. The two movable baffles are respectively provided with limiting inclined surfaces that match the first abutting surface and the second abutting surface.
[0011] As one embodiment, the mounting base is provided with a first locking part and a second locking part, the first locking part being used to lock the finger stop of the syringe, and the second locking part being used to lock the syringe barrel.
[0012] As one embodiment, the first latching portion includes two first latching plates arranged radially spaced along the syringe, with a placement space for placing the syringe between the two first latching plates, and the upper side of the first latching plate abutting against the lower side of the finger stop.
[0013] As one embodiment, the first latching portion further includes a second latching plate, two second latching plates are spaced apart, the two second latching plates are disposed on the upper side of the first latching plate, the second latching plates are spaced apart from the first latching plate, and there is a receiving space between the second latching plate and the first latching plate for accommodating the finger stop, the finger stop and the receiving space are interference fit.
[0014] As one embodiment, the second locking portion includes a first locking claw and a second locking claw, the first locking claw and the second locking claw are spaced apart, the upper end of the first locking claw extends toward the second locking claw, the upper end of the second locking claw extends toward the first locking claw, the distance between the upper end of the first locking claw and the upper end of the second locking claw is less than the diameter of the syringe, and the distance between the lower end of the first locking claw and the lower end of the second locking claw is greater than the diameter of the syringe.
[0015] As one embodiment, the first jaw and / or the second jaw are provided with connecting holes, the axis of the connecting holes is parallel to the axis of the syringe, and a connecting block is provided on the side wall of the syringe, the connecting block being provided with a through hole that matches the connecting holes.
[0016] This invention also provides a method for preparing a microdroplet extrusion-type bio-3D printing device, comprising the following steps: The second adjustment component is installed on the drive end of the first adjustment component; The lead screw assembly and syringe are mounted on the drive end of the second adjustment assembly; Connect the syringe plunger to the lead screw assembly.
[0017] The present invention achieves the following technical effects compared to the prior art: In the microdroplet extrusion-type bio-3D printing device disclosed in this invention, the position of the syringe can be adjusted by the first adjustment component and the second adjustment component to control the spacing of the extruded microdroplets. The syringe plunger is connected to the lead screw assembly, and the lead screw assembly drives the syringe plunger to move. Compared with pneumatic pressure drive or mechanical push rod drive, the lead screw assembly has higher transmission precision and more stable transmission speed, ensuring the uniform distribution of shear force during extrusion, avoiding problems such as microdroplet adhesion, uneven size, and disordered spacing, and improving the uniformity of the size and spacing of the extruded microdroplets. Attached Figure Description
[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0019] Figure 1 This is a schematic diagram of a microdroplet extrusion-type bio-3D printing device in an embodiment of the present invention; Figure 2 for Figure 1 A diagram from another perspective; Figure 3 This is a schematic diagram of the installation of the culture plate in an embodiment of the present invention; Figure 4 This is a schematic diagram of the syringe installation in an embodiment of the present invention; Figure 5 This is a schematic diagram of the lead screw assembly in an embodiment of the present invention.
[0020] The components include: 1. First adjusting component; 2. Second adjusting component; 3. Syringe; 4. Push handle; 5. Mounting base; 6. Angle adjusting component; 7. Third adjusting component; 8. Culture plate; 9. Third slide rod; 10. Third slide block; 11. Third slide groove; 12. Limiting plate; 13. Movable baffle; 14. Snap-fit protrusion; 15. First abutment surface; 16. Second abutment surface; 17. Finger stop; 18. First locking plate; 19. Second locking plate; 20. First claw; 21. Second claw; 22. Support base; 23. First slide rod; 24. First slide block; 25. First slide groove; 26. Second slide rod; 27. Second slide block; 28. Second slide groove; 29. Screw; 30. Slider; 31. Guide rail. Detailed Implementation
[0021] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0022] The purpose of this invention is to provide a microdroplet extrusion-type bio-3D printing device and its preparation method to solve the problems existing in the prior art. By driving the push handle of the syringe through the lead screw assembly, the uniform distribution of shear force during the extrusion process is ensured, thereby improving the uniformity of the size and spacing of the extruded microdroplets.
[0023] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
[0024] Example 1 Please refer to Figures 1-5This embodiment provides a microdroplet extrusion bio-3D printing device, including: a first adjustment component 1, a second adjustment component 2, a syringe 3, and a lead screw assembly. The first adjustment component 1 is vertically arranged and is used to drive the object to move in the vertical direction. The second adjustment component 2 is horizontally installed on the first adjustment component 1 and is used to drive the object to move in the horizontal direction. The syringe 3 is installed on the second adjustment component 2, and the plunger 4 of the syringe 3 is connected to the lead screw assembly. Its working principle is as follows: the second adjustment component 2 is installed on the drive end of the first adjustment component 1. The first adjustment component 1 can drive the second adjustment component 2 to move in the vertical direction. The syringe 3 is installed on the drive end of the second adjustment component 2. The second adjustment component 2 can drive the syringe 3 to move in the horizontal direction. Thus, the spatial position of the syringe 3 can be adjusted by the first adjustment component 1 and the second adjustment component 2 to control the spacing of the extruded microdroplets. The screw assembly includes a screw 29 and a slider 30. The screw 29 is connected to the drive motor. The slider 30 has a threaded hole that matches the screw 29. When the screw 29 rotates, the slider 30 can drive the push handle 4 of the syringe 3 to move along the axial direction of the screw 29, thereby extruding ink from the syringe 3 to form the required microdroplets. Compared with pneumatic pressure drive or mechanical push rod drive, the screw assembly has higher transmission accuracy and smoother transmission speed, ensuring the uniform distribution of shear force during extrusion, avoiding problems such as microdroplet adhesion, uneven size, and disordered spacing, improving the uniformity of the size and spacing of the extruded microdroplets, and reducing damage to cells.
[0025] In one embodiment, the slider 30 is connected to the plunger 4 of the syringe 3 by bolts.
[0026] In one embodiment, a control system is also included, which includes an embedded control motherboard, a touch interaction unit, and microdroplet parameter closed-loop adjustment software; the control system is able to receive instructions from the slicing software, control the extrusion speed and extrusion time, and the spatial position of the syringe 3.
[0027] In one embodiment, the first adjustment component 1 includes a first driving device, a first slide rod 23 and a first slide block 24. The first slide rod 23 has a horizontally arranged first slide groove 25, and the first slide block 24 is slidably installed in the first slide groove 25. The first slide block 24 serves as the driving end of the first adjustment component 1.
[0028] In one embodiment, the microdroplet extrusion bio-3D printing device further includes an angle adjustment element 6, which is mounted on the second adjustment assembly 2. The mounting base 5 is mounted on the angle adjustment element 6, and the angle adjustment element 6 can adjust the angle of the syringe 3.
[0029] In one embodiment, the angle adjustment component 6 is a drive motor. The motor mount of the drive motor is installed on the drive end of the second adjustment component 2. The mounting base 5 is fixedly installed on the output shaft of the drive motor. The drive motor can adjust the angle of the mounting base 5 by rotating, thereby realizing the adjustment of the angle of the syringe 3.
[0030] In one embodiment, the angle adjusting component 6 includes a mounting plate, which is installed on the drive end of the second adjusting component 2. The mounting plate has a transition hole, and the mounting seat 5 is rotatably installed in the transition hole via a pin. The mounting plate also has multiple adjusting holes, and the mounting seat 5 is provided with a limit hole. The adjusting hole is located on the rotation path of the limit hole when it rotates around the transition hole. The size of the limit hole matches the size of the adjusting hole. When the limit hole and the adjusting hole are aligned, the mounting seat 5 can be fixed at a specific angle by inserting a limit rod into the limit hole and the adjusting hole.
[0031] In one embodiment, the second adjustment component 2 includes a second driving device, a second slide rod 26, and a second slide block 27. The second slide rod 26 has a horizontally arranged second slide groove 28, and the second slide block 27 is slidably installed in the second slide groove 28. The second slide block 27 serves as the driving end of the second adjustment component 2.
[0032] In one embodiment, the microdroplet extrusion bio-3D printing device further includes a third adjustment component 7 and a culture plate 8. The third adjustment component 7 is horizontally positioned, and its adjustment direction is perpendicular to that of the second adjustment component 2. The culture plate 8 is mounted on the third adjustment component 7. The culture plate 8 is used to receive the microdroplets extruded by the syringe 3. The third adjustment component 7 can drive the culture plate 8 to move, thereby achieving triaxial adjustment of the relative position between the syringe 3 and the culture plate 8, simplifying the movement path of the syringe 3.
[0033] In one embodiment, PLCL fibers are laid on the culture plate 8.
[0034] In one embodiment, the third adjustment component 7 includes a third slide rod 9 and a third slide block 10. The third slide rod 9 has a third slide groove 11, and the third slide block 10 is slidably installed in the third slide groove 11. The third slide block 10 can be driven to slide within the third slide groove 11 by a third driving device. The third adjustment component 7 is configured in the same way as the first adjustment component 1.
[0035] In one embodiment, the limiting mechanism includes a limiting plate 12 and two movable baffles 13. The limiting plate 12 is fixedly disposed on the upper side of the third slide 10, and the limiting plate 12 and the third slide 10 are spaced apart. The two movable baffles 13 are spaced apart on the third slide 10 and are rotatably connected to the third slide 10. The bottom of the culture plate 8 is provided with an outwardly facing snap-fit protrusion 14. The distance between the limiting plate 12 and the third slide 10 matches the thickness of the snap-fit protrusion 14. The side wall of the limiting plate 12 is provided with a first abutment surface 15 and a second abutment surface 16. The end of the first abutment surface 15 away from the limiting plate 12 is inclined toward the second abutment surface 16, and the end of the second abutment surface 16 away from the limiting plate 12 is inclined toward the first abutment surface 15. The two movable baffles 13 are respectively provided with limiting inclined surfaces that match the first abutment surface 15 and the second abutment surface 16. There is a limiting space between the limiting plate 12 and the third slide 10 for accommodating the culture plate 8. When the culture plate 8 is installed on the third slide 10, the snap-fit protrusion 14 at the bottom of the culture plate 8 is inserted into the limiting space. At this time, the side of the snap-fit protrusion 14 abuts against the extension of the supporting limiting plate 12, or the side wall of the limiting plate 12 near the culture plate 8 abuts against the culture plate 8. The two movable baffles 13 are rotated respectively, so that the limiting inclined surfaces on the two movable baffles 13 abut against the first abutting surface 15 and the second abutting surface 16 respectively, thereby enabling the culture plate 8 to be installed on the third slide 10. When it is necessary to remove the culture plate 8, the two movable baffles 13 are rotated in the opposite direction to pull the culture plate 8 out of the limiting space. In this way, the stability of the culture plate 8 is ensured while the ease of disassembly of the culture plate 8 is improved.
[0036] In one embodiment, the movable baffle 13 and the third slide 10 are spaced apart, and the distance between the movable baffle 13 and the third slide 10 is equal to the thickness of the snap-fit protrusion 14, so that the movable baffle 13 can provide vertical limitation for the culture plate 8, further improving the stability of the culture plate 8.
[0037] In one embodiment, the limiting mechanism includes four baffles, two baffles forming a group, which are spaced apart on the third slide block 10. The line connecting the two baffles in the first group is perpendicular to the line connecting the two baffles in the second group. The culture plate 8 has a rectangular structure, so that the four baffles can provide support forces in four directions for the culture plate 8 to be installed on the third slide groove 11.
[0038] In one embodiment, the limiting mechanism may also be a known connection structure such as a limiting buckle.
[0039] In one embodiment, the mounting base 5 is provided with a first locking part and a second locking part. The first locking part is used to lock the finger stop 17 of the syringe 3, and the second locking part is used to lock the syringe barrel of the syringe 3. The syringe 3 can be installed on the mounting base 5 through the second locking part, and the first locking part can provide support for the finger stop 17 of the syringe 3. When the screw assembly pushes the plunger 4 of the syringe 3 to move, the first locking part can keep the syringe barrel of the syringe 3 in the original position through the finger stop 17, thereby completing the process of extruding microdroplets.
[0040] In one embodiment, the first locking portion includes two first locking plates 18 arranged radially spaced along the syringe 3, with a placement space between the two first locking plates 18 for placing the syringe 3. The upper side of the first locking plate 18 is used to abut against the lower side of the finger stop 17. The "upper side" referred to here is the upper side of the first locking plate 18 when the syringe 3 is in use, that is, the side of the first locking plate 18 away from the liquid outlet end of the syringe 3. This method can provide support for both finger stops 17 simultaneously to ensure the stability of the syringe 3 during the push rod movement.
[0041] In one embodiment, the first locking portion further includes a second locking plate 19. Two second locking plates 19 are spaced apart to ensure that the syringe 3 can be inserted between them. The two second locking plates 19 are positioned above the first locking plate 18, spaced apart from it. A receiving space for accommodating a finger stop 17 is provided between the second locking plates 19 and the first locking plate 18, and the finger stop 17 is interference-fitted into the receiving space. The engagement of the first locking plate 18 and the second locking plate 19 secures the finger stop 17, thereby improving the stability of the syringe 3.
[0042] In one embodiment, the second locking portion includes a first locking claw 20 and a second locking claw 21, the first locking claw 20 and the second locking claw 21 are spaced apart, the upper end of the first locking claw 20 extends toward the second locking claw 21, the upper end of the second locking claw 21 extends toward the first locking claw 20, the distance between the upper end of the first locking claw 20 and the upper end of the second locking claw 21 is less than the diameter of the syringe 3, and the distance between the lower end of the first locking claw 20 and the lower end of the second locking claw 21 is greater than the diameter of the syringe 3. Both the first jaw 20 and the second jaw 21 can produce a certain amount of elastic deformation. In use, the syringe is inserted into the space between the first jaw 20 and the second jaw 21 through the gap between the upper ends of the first jaw 20 and the second jaw 21. During the insertion process, the syringe provides a supporting force to the first jaw 20 and the second jaw 21 to keep them away from each other. The first jaw 20 and the second jaw 21 can produce elastic deformation to allow the syringe to enter the space between them. After the syringe enters the space between them, it provides a pushing force to the syringe pointing to the midpoint of the line connecting the first jaw 20 and the second jaw 21, so that the syringe can be installed on the mounting base 5. When it is necessary to remove the syringe, simply pull it out in the opposite direction.
[0043] In one embodiment, in order to facilitate inserting the syringe between the first clamping claw 20 and the second clamping claw 21, the upper ends of both the first clamping claw 20 and the second clamping claw 21 are rounded.
[0044] In one embodiment, the first jaw 20 and / or the second jaw 21 are provided with connecting holes, the axis of which is parallel to the axis of the syringe 3. A connecting block is provided on the side wall of the syringe 3, and the connecting block is provided with a through hole that matches the connecting hole. The connecting hole and the through hole on the connecting block can be connected together by a pin or bolt, thereby further improving the stability of the syringe 3 during use.
[0045] In one embodiment, a support seat 22 is also provided on the third slide 10. The support seat 22 is located between the first claw 20 and the second claw 21. The support seat 22 can support the syringe 3 so that after the syringe 3 enters between the first claw 20 and the second claw 21, neither the first claw 20 nor the second claw 21 will return to its initial position. Instead, it can continuously provide squeezing force to the syringe 3, thus preventing the syringe 3 from shaking between the first claw 20 and the second claw 21.
[0046] In one embodiment, a V-shaped groove is provided on the upper surface of the support base 22. The V-shaped groove can limit the position of the syringe 3, thereby further improving the stability of the syringe 3.
[0047] In one embodiment, multiple first claws 20 and second claws are provided to further improve the stability of the syringe 3.
[0048] In one embodiment, the microdroplet extrusion bio-3D printing device also includes an integrated microscope for monitoring the real-time location and morphology of the microdroplets.
[0049] In one embodiment, the microdroplet extrusion bio-3D printing device further includes a grating positioning component, which is capable of detecting and positioning the position and angle of the syringe 3.
[0050] In one embodiment, the third slide block 10 is also provided with a guide rail 31, and the slider 30 is provided with a groove corresponding to the guide rail 31. The guide rail 31 and the groove can further improve the stability of the slider 30 during movement.
[0051] In one embodiment, the first drive device, the second drive device, and the third drive device can all be a lead screw and nut mechanism or a stepper motor.
[0052] Example 2 This embodiment provides a bio-3D printing method using the aforementioned microdroplet extrusion bio-3D printing device, comprising the following steps: loading ink into a syringe 3 inside a biosafety cabinet; mounting the syringe 3 onto a second adjustment component 2; activating the lead screw assembly to drive the push handle 4 of the syringe 3 to extrude ink microdroplets; and moving the first adjustment component 1 and the second adjustment component 2 along a preset path to deposit the microdroplets at a preset interval to the target position.
[0053] In one embodiment, the microdroplet extrusion bio-3D printing device is sterilized with ultraviolet light before the syringe 3 is installed onto the second adjustment assembly 2.
[0054] In one embodiment, a pure microfluidic focusing structure or vibration-assisted droplet breaking is used to achieve uniform microdroplets.
[0055] Example 3 This embodiment provides a method for preparing a microdroplet extrusion-based bio-3D printing device, which, using the aforementioned microdroplet extrusion-based bio-3D printing device, includes the following steps: The second adjustment component 2 is installed on the drive end of the first adjustment component 1; Install the lead screw assembly and syringe 3 on the drive end of the second adjustment assembly 2; Connect the plunger of syringe 3 to the lead screw assembly.
[0056] Specific examples have been used to illustrate the principles and implementation methods of this invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this invention. Furthermore, those skilled in the art will recognize that, based on the ideas of this invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of this invention.
Claims
1. A microdroplet extrusion-based bio-3D printing device, characterized in that, include: The first adjustment component (1) is vertically set and is used to drive the object to move in the vertical direction; The second adjustment component (2) is horizontally mounted on the first adjustment component (1) and is used to drive the object to move in the horizontal direction. A syringe (3) is mounted on the second adjustment assembly (2); And a lead screw assembly, wherein the plunger (4) of the syringe (3) is connected to the lead screw assembly.
2. The microdroplet extrusion-type bio-3D printing device according to claim 1, characterized in that, It also includes a mounting base (5) and an angle adjustment component (6), the angle adjustment component (6) being mounted on the second adjustment assembly (2), the mounting base (5) being mounted on the angle adjustment component (6), the syringe (3) being mounted on the mounting base (5), and the angle adjustment component (6) being able to adjust the angle of the mounting base (5).
3. The microdroplet extrusion-type bio-3D printing device according to claim 1, characterized in that, It also includes a third adjustment component (7) and a culture plate (8). The third adjustment component (7) is horizontally arranged, and the adjustment direction of the third adjustment component (7) is perpendicular to the adjustment direction of the second adjustment component (2). The culture plate (8) is installed on the third adjustment component (7). The third adjustment component (7) includes a third slide rod (9) and a third slide block (10). A third slide groove (11) is provided on the third slide rod (9). The third slide block (10) is slidably installed in the third slide groove (11). A limit mechanism is provided on the third slide block (10) to detachably install the culture plate (8) on the third slide block (10).
4. The microdroplet extrusion-type bio-3D printing device according to claim 3, characterized in that, The limiting mechanism includes a limiting plate (12) and two movable baffles (13). The limiting plate (12) is fixedly disposed on the upper side of the third slide (10). The limiting plate (12) and the third slide (10) are spaced apart. The two movable baffles (13) are spaced apart on the third slide (10) and rotatably connected to the third slide (10). The bottom of the culture plate (8) is provided with an outward snap-fit protrusion (14). The distance between the limiting plate (12) and the third slide (10) is equal to the distance between the snap-fit protrusion and the third slide (10). The thickness of the plate (14) is matched. The side wall of the limiting plate (12) is provided with a first abutting surface (15) and a second abutting surface (16). The end of the first abutting surface (15) away from the limiting plate (12) is inclined towards the second abutting surface (16). The end of the second abutting surface (16) away from the limiting plate (12) is inclined towards the first abutting surface (15). The two movable baffles (13) are respectively provided with limiting inclined surfaces that match the first abutting surface (15) and the second abutting surface (16).
5. The microdroplet extrusion-type bio-3D printing device according to claim 1, characterized in that, The mounting base (5) is provided with a first locking part and a second locking part. The first locking part is used to lock the finger stop (17) of the syringe (3), and the second locking part is used to lock the syringe (3) syringe.
6. The microdroplet extrusion-type bio-3D printing device according to claim 5, characterized in that, The first latching part includes two first latching plates (18) arranged radially spaced along the syringe (3), with a placement space between the two first latching plates (18) for placing the syringe (3), and the upper side of the first latching plate (18) abutting against the lower side of the finger stop (17).
7. The microdroplet extrusion-type bio-3D printing device according to claim 6, characterized in that, The first latching part further includes a second latching plate (19), two second latching plates (19) are spaced apart, the two second latching plates (19) are disposed on the upper side of the first latching plate (18), the second latching plates (19) are spaced apart from the first latching plate (18), and there is a receiving space between the second latching plate (19) and the first latching plate (18) for accommodating the finger stop (17), and the finger stop (17) is interference-fitted with the receiving space.
8. The microdroplet extrusion-type bio-3D printing device according to claim 5, characterized in that, The second locking part includes a first locking claw (20) and a second locking claw (21). The first locking claw (20) and the second locking claw (21) are spaced apart. The upper end of the first locking claw (20) extends toward the second locking claw (21), and the upper end of the second locking claw (21) extends toward the first locking claw (20). The distance between the upper end of the first locking claw (20) and the upper end of the second locking claw (21) is less than the diameter of the syringe (3), and the distance between the lower end of the first locking claw (20) and the lower end of the second locking claw (21) is greater than the diameter of the syringe (3).
9. The microdroplet extrusion-type bio-3D printing device according to claim 8, characterized in that, The first claw (20) and / or the second claw (21) are provided with connecting holes, the axis of the connecting holes is parallel to the axis of the syringe (3), and a connecting block is provided on the side wall of the syringe (3), and a through hole matching the connecting hole is provided on the connecting block.
10. A method for preparing a microdroplet extrusion-based bio-3D printing device, characterized in that, Includes the following steps: Install the second adjustment component (2) on the drive end of the first adjustment component (1); Install the lead screw assembly and syringe (3) on the drive end of the second adjustment assembly (2); Connect the plunger of the syringe (3) to the lead screw assembly.