A viral load detection device for HBV-related liver cancer research

Abstract

The application discloses a virus load detection device for HBV-related liver cancer research and belongs to the field of liver cancer research equipment, which is used for solving the problem that liver cancer samples are viscous and easy to cause subsequent pipeline blockage; the device comprises a machine body, a sample processing module and a nucleic acid extraction module are installed on the machine body, a filter module is connected between the sample processing module and the nucleic acid extraction module, the filter module comprises a sample adding pipe and a transfer pipe, the transfer pipe is connected between the sample processing module and the nucleic acid extraction module, the sample adding pipe is connected to the middle part of the transfer pipe, one-way valves are arranged on the sample adding pipe and the transfer pipe, a mounting shell is arranged on the transfer pipe, a filter screen is rotatably connected in the mounting shell, when the sample adding pipe adds lysing solution into the sample processing module, the filter screen does not rotate, and when the sample in the sample processing module is transferred to the nucleic acid extraction module through the transfer pipe, the filter screen rotates to remove filter tissue residues; the device can filter the sample, so that the tissues in the sample are separated, and subsequent pipeline blockage is avoided.

Description

Technical Field

[0001] This invention relates to the field of liver cancer research equipment technology, specifically to a viral load detection device for HBV-related liver cancer research. Background Technology

[0002] In basic research and clinical sample analysis of HBV-related liver cancer, accurate quantitative detection of HBV viral load is a core step in exploring the correlation between viral load and tumor proliferation, inflammatory microenvironment, and patient prognosis, and is also an important basis for evaluating the efficacy of antiviral therapy. Viral load detection requires pretreatment of the sample, such as lysis and inactivation, and the extraction and purification of HBV-DNA. Therefore, the relevant detection device needs to achieve efficient integration of sample pretreatment and nucleic acid extraction to ensure detection efficiency and result accuracy.

[0003] Most existing HBV viral load detection devices directly connect the sample processing module and the nucleic acid extraction module through tubing to complete sample transfer and subsequent extraction operations. However, in practical applications for HBV-related liver cancer research, significant compatibility defects have been found: liver cancer research often uses viscous samples such as liver tissue homogenate and xenograft tissue homogenate. These samples contain a large amount of tissue residue, which can easily cause tubing blockage when transferred directly, leading to sample transfer interruption and nucleic acid extraction module injection failure. This not only affects the continuity of the detection process but also causes sample waste. Especially for trace samples such as mouse xenografts, sample loss due to blockage can directly lead to detection failure.

[0004] To address these issues, some researchers manually filter samples after pretreatment before transferring them to the nucleic acid extraction module. While this removes tissue residue, it adds an extra manual step, reducing detection efficiency and increasing the risk of nucleic acid loss and cross-contamination during manual transfer. Furthermore, the inconsistent nature of manual filtering means that different researchers' filtering methods can lead to variations in sample processing results, affecting the reproducibility of viral load detection and making it difficult to meet the standardized, batch-based sample testing needs in HBV-related liver cancer research.

[0005] Some testing devices also add fixed filters in the sample transmission pipeline for filtration. However, the filter surface of the fixed filter is fixed, and tissue residue in viscous samples can easily adhere to a single position on the filter, causing a decrease in filter permeability in a short time, or even filter blockage. This still cannot fundamentally solve the pipeline blockage problem. Moreover, after the filter is blocked, it is necessary to stop the machine for disassembly and cleaning, which further reduces the efficiency of the testing device. Summary of the Invention

[0006] The purpose of this invention is to provide a viral load detection device for HBV-related liver cancer research, which solves the problem that the viscosity of liver cancer samples easily leads to subsequent pipeline blockage.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a viral load detection device for HBV-related liver cancer research, comprising a body, wherein a sample processing module and a nucleic acid extraction module are installed on the body, and a filtration module is connected between the sample processing module and the nucleic acid extraction module. The filtration module includes a sample dispensing tube and a transfer tube, wherein the transfer tube is connected between the sample processing module and the nucleic acid extraction module, and the sample dispensing tube is connected to the middle of the transfer tube. Both the sample dispensing tube and the transfer tube are provided with a one-way valve. The transfer tube is provided with a mounting shell, and a filter screen is rotatably connected inside the mounting shell. When the sample dispensing tube adds lysis buffer to the sample processing module, the filter screen does not rotate. Furthermore, when the sample in the sample processing module is transferred to the nucleic acid extraction module through the transfer tube, the filter screen rotates to move and filter tissue residue.

[0008] Preferably, the filtration module further includes a dosing pump connected to the transfer tube, and the sample processing module is equipped with a motor for driving the dosing pump.

[0009] Preferably, a mounting shaft is fixedly connected to the middle of the filter screen, and a rotating shaft is connected to the end of the mounting shaft through a one-way bearing. A driven bevel gear is installed at the end of the rotating shaft, and a driving bevel gear that meshes with the driven bevel gear is installed at the output end of the motor. When the motor rotates forward, the injection pump draws external lysis buffer through the sample tube, and the rotating shaft rotates relative to the mounting shaft. When the motor rotates in reverse, the injection pump draws the processed sample and transfers it to the nucleic acid extraction module through the transfer tube, and the rotating shaft drives the mounting shaft to rotate synchronously.

[0010] Preferably, a scraper is fixedly connected to the inner wall of the mounting housing, and the scraper is in contact with the filter screen.

[0011] Preferably, the sample processing module includes a temperature control chamber, a tube rack is placed inside the temperature control chamber, test tubes containing tissue samples are placed on the tube rack, and the inner wall of the temperature control chamber is provided with protrusions that can support the tube rack.

[0012] Preferably, the sample processing module further includes a top plate, on which multiple small motors are fixedly connected. Sterile grinding heads are installed at the output ends of the multiple small motors. Multiple branch tubes are connected to the top plate near the multiple sterile grinding heads. The multiple branch tubes are all connected to the sample dispensing tube. The up and down movement of the top plate can drive the sterile grinding heads and the branch tubes to enter the corresponding test tubes synchronously.

[0013] Preferably, the nucleic acid extraction module includes an extraction box, the top of which is fitted with a cover plate. The cover plate has a cavity communicating with the transfer tube. The lower surface of the cover plate has multiple liquid outlet holes communicating with the cavity. Multiple magnetic microspheres are fixedly installed below the multiple liquid outlet holes.

[0014] Preferably, a rectangular plate is fixedly connected inside the extraction box, and a fixing frame is fixedly connected inside the extraction box. Multiple magnetic microspheres are fixedly connected to the fixing frame. The rectangular plate has conical holes corresponding to the multiple magnetic microspheres. After the sample drips through the liquid outlet hole, it can be guided to drip onto the magnetic microspheres through the conical holes.

[0015] Preferably, a transfer box is slidably connected to the middle of the extraction box. The transfer box has a first cavity and a second cavity. The first cavity is used to receive waste liquid, and the second cavity is used to receive the processed sample. The outer wall of the extraction box is rotatably connected to a cylindrical gear, and the transfer box is connected to a rack that meshes with the cylindrical gear.

[0016] Preferably, the rectangular plate is connected to multiple diversion tubes, which are connected to an eluent pipeline. An L-shaped frame is fixedly connected to the side of the rectangular plate, and a press valve is installed on the L-shaped frame. The press valve is installed in the eluent pipeline. The extraction box is provided with a stop block that cooperates with the press valve. When the transfer box moves so that the second cavity is directly below the rectangular plate, the transfer box can push the rectangular plate to slide through the L-shaped frame, so that the diversion tubes are directly aligned with the magnetic microspheres, and the press valve contacts the stop block, thereby opening the eluent pipeline. Both the first cavity and the second cavity are connected to an exhaust pipe, and the exhaust pipe on the second cavity is connected to a fluorescence detection device.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention addresses the issue that when the lysis buffer enters the sample processing module, the filter in the filtration module does not rotate, and therefore does not perform filtration. Instead, when the sample processed by the sample processing module enters the nucleic acid extraction module through the transfer tube, the filter filters the sample flowing in the transfer tube, removing tissue residues. Simultaneously, the filter rotates, ensuring that its surface fully participates in filtration, thus preventing the filter from continuously performing filtration at a single location and affecting permeability. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2This is a schematic diagram of the sample processing module and nucleic acid extraction module of the present invention; Figure 3 This is a schematic diagram of the sample processing module of the present invention; Figure 4 This is a schematic diagram of the structure of the pipe rack in this invention; Figure 5 This is a schematic diagram of the structure of the filtering module of the present invention; Figure 6 This is a schematic diagram of the structure at the rotating shaft of the present invention; Figure 7 This is a schematic diagram of the structure of the nucleic acid extraction module of the present invention; Figure 8 This is a schematic diagram of the structure of the fixing frame of the present invention.

[0019] In the diagram: 100, Main body; 200, Sample processing module; 210, Temperature control chamber; 220, Protrusion; 230, Tube rack; 240, Top plate; 250, Sterile grinding head; 251, Small motor; 260, Branch tube; 300, Filter module; 310, Sample dispensing tube; 320, Transfer tube; 330, Dosing pump; 340, Motor; 341, Driving bevel gear; 350, Rotating shaft; 351, Driven bevel gear; 352, One-way bearing; 353, Mounting shaft; 360, Mounting housing; 3 61. Filter screen; 362. Scraper; 400. Nucleic acid extraction module; 410. Extraction box; 420. Cover plate; 421. Liquid outlet; 430. Rectangular plate; 431. Conical hole; 432. Diverter tube; 440. L-shaped frame; 441. Elution fluid pipeline; 442. Press valve; 443. Stop block; 450. Transfer box; 451. First chamber; 452. Second chamber; 453. Discharge pipe; 460. Rack; 470. Cylindrical gear; 480. Fixing frame; 481. Magnetic microspheres. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] Reference Figures 1-8This embodiment provides a technical solution: a viral load detection device for HBV-related liver cancer research, including a body 100, a sample processing module 200 and a nucleic acid extraction module 400 installed on the body 100, a filter module 300 connected between the sample processing module 200 and the nucleic acid extraction module 400, the filter module 300 including a sample dispensing tube 310 and a transfer tube 320, the transfer tube 320 being connected between the sample processing module 200 and the nucleic acid extraction module 400, the sample dispensing tube 310 being connected to the middle of the transfer tube 320, both the sample dispensing tube 310 and the transfer tube 320 being provided with a one-way valve, the transfer tube 320 being provided with a mounting shell 360, a filter screen 361 being rotatably connected inside the mounting shell 360, when the sample dispensing tube 310 adds lysis buffer into the sample processing module 200, the filter screen 361 does not rotate, and when the sample in the sample processing module 200 is transferred to the nucleic acid extraction module 400 through the transfer tube 320, the filter screen 361 rotates to move and filter tissue residue.

[0022] The sample is processed by the sample processing module 200, such as lysis and inactivation. During sample lysis, lysis buffer is injected through the sample addition tube 310. The processed sample is transferred to the nucleic acid extraction module 400 through the transfer tube 320. Due to the viscous properties of liver cancer tissue homogenate sample, a filter module 300 is set in the device to avoid subsequent pipeline blockage. The entry of the lysis buffer and the exit of the sample are driven by a power source. When the lysis buffer enters the sample processing module 200, the filter screen 361 of the filtration module 300 does not rotate, and the filter screen 361 does not perform filtration at this time. When the sample processed by the sample processing module 200 enters the nucleic acid extraction module 400 through the transfer tube 320, the filter screen 361 filters the sample flowing in the transfer tube 320 to remove tissue residues in the sample. At the same time, the filter screen 361 rotates so that its surface can fully participate in filtration, avoiding the continuous filtration action of a single position of the filter screen 361, which would affect the permeability.

[0023] The filter module 300 also includes a dosing pump 330 connected to the transfer tube 320, and the sample processing module 200 is equipped with a motor 340 for driving the dosing pump 330.

[0024] When the motor 340 is running, it can drive the injection pump 330 to run. The injection pump 330 adopts a gear pump or a peristaltic pump to ensure that the injection pump 330 can run when the motor 340 rotates forward or reverse. The injection pump 330 can change its delivery direction with the forward and reverse rotation of the motor 340. Thus, by setting up an injection pump 330 in conjunction with the sample tube 310, the transfer tube 320 and the one-way valve, the lysis solution can be introduced and the sample can be transferred.

[0025] A mounting shaft 353 is fixedly connected to the middle of the filter 361. The end of the mounting shaft 353 is connected to a rotating shaft 350 via a one-way bearing 352. A driven bevel gear 351 is mounted on the end of the rotating shaft 350. An active bevel gear 341 that meshes with the driven bevel gear 351 is mounted on the output end of the motor 340. When the motor 340 rotates forward, the injection pump 330 draws external lysis buffer through the sample tube 310, and the rotating shaft 350 rotates relative to the mounting shaft 353. When the motor 340 rotates in reverse, the injection pump 330 draws the processed sample and transfers it to the nucleic acid extraction module 400 through the transfer tube 320, and the rotating shaft 350 drives the mounting shaft 353 to rotate synchronously.

[0026] When the motor 340 rotates forward, it can drive the injection pump 330 to run in the forward direction, so that the lysis solution is drawn into the sample processing module 200 through the sample tube 310. At this time, the driving bevel gear 341 on the motor 340 drives the driven bevel gear 351 to rotate, thereby rotating the shaft 350. The one-way bearing 352 connected between the shaft 350 and the mounting shaft 353 allows the shaft 350 to rotate relative to the mounting shaft 353. At this time, the filter screen 361 will not rotate. When the motor 340 reverses, the delivery direction of the pump 330 is reversed, allowing the sample to be drawn in the opposite direction to the nucleic acid extraction module 400. The one-way valve ensures the directional delivery of the liquid. At the same time, the rotation direction of the rotating shaft 350 is also reversed, so the rotating shaft 350 drives the mounting shaft 353 to rotate synchronously through the one-way bearing 352. At this time, the filter screen 361 rotates and filters the sample. During the movement, the surface of the filter screen 361 can fully participate in filtration, effectively alleviating clogging.

[0027] A scraper 362 is fixedly connected to the inner wall of the mounting housing 360, and the scraper 362 is attached to the filter screen 361.

[0028] Two sets of scraper blocks 362 can be set up. The two sets of scraper blocks 362 scrape both sides of the filter screen 361, so that the tissues adhering to the filter screen 361 can be scraped off, thus preventing the filter screen 361 from being blocked.

[0029] The sample processing module 200 includes a temperature control chamber 210, a tube rack 230 is placed inside the temperature control chamber 210, and test tubes containing tissue samples are placed on the tube rack 230. The inner wall of the temperature control chamber 210 is provided with protrusions 220, which can support the tube rack 230.

[0030] The temperature control chamber 210 can be used to heat the sample in the test tube for water bath heating for lysis and low-temperature preservation by introducing water. The temperature control chamber 210 is equipped with a semiconductor heating and cooling chip to ensure easy control of the internal water temperature. The protrusion 220 supports the tube rack 230, and the test tube is placed in the tube rack 230 to ensure that the test tube is firmly fixed.

[0031] The sample processing module 200 also includes a top plate 240, on which multiple small motors 251 are fixedly connected. Sterile grinding heads 250 are installed at the output ends of the multiple small motors 251. Multiple branch tubes 260 are connected to the top plate 240 near the multiple sterile grinding heads 250. The multiple branch tubes 260 are all connected to the sample dispensing tube 310. The up and down movement of the top plate 240 can drive the sterile grinding heads 250 and the branch tubes 260 to enter the corresponding test tubes simultaneously.

[0032] The top plate 240 can be connected to the temperature control box 210 via an electric telescopic rod. By controlling the extension and retraction of the electric telescopic rod, the top plate 240 can be moved closer to or further away from the temperature control box 210. After the test tube containing the sample is placed, the top plate 240 is lowered. At this time, the sterile grinding head 250 enters the test tube, and the branch tube 260 also enters the test tube. The branch tube 260 injects lysis buffer into the test tube, and the sterile grinding head 250 rotates and grinds the internal sample, which facilitates the full release of the effective components in the sample.

[0033] The nucleic acid extraction module 400 includes an extraction box 410. A cover plate 420 is installed on the top of the extraction box 410. A cavity communicating with a transfer tube 320 is opened inside the cover plate 420. Multiple liquid outlet holes 421 communicating with the cavity are opened on the lower surface of the cover plate 420. Multiple magnetic microspheres 481 are fixedly installed at the lower part of the multiple liquid outlet holes 421.

[0034] The sample processed by the sample processing module 200 enters the cavity inside the cover plate 420 through the transfer tube 320, and then drips onto the magnetic microspheres 481 through the liquid outlet 421. The magnetic microspheres 481 have HBV nucleic acid specificity and can directionally adsorb HBV-DNA with an extraction efficiency of ≥95%.

[0035] A rectangular plate 430 is fixedly connected inside the extraction box 410, and a fixing frame 480 is fixedly connected inside the extraction box 410. Multiple magnetic microspheres 481 are fixedly connected to the fixing frame 480. The rectangular plate 430 has conical holes 431 that correspond one-to-one with the multiple magnetic microspheres 481. After the sample drips through the liquid outlet hole 421, it can be guided to drip onto the magnetic microspheres 481 through the conical holes 431.

[0036] The upper surface of the rectangular plate 430 receives excess sample, and the conical hole 431 on the rectangular plate 430 can also guide the sample. The magnetic microsphere 481 is located directly below the small diameter end of the conical hole 431 and is relatively close, thus ensuring that the sample can fall exactly onto the magnetic microsphere 481.

[0037] The extraction box 410 is slidably connected to the middle of the transfer box 450. The transfer box 450 has a first cavity 451 and a second cavity 452. The first cavity 451 is used to receive waste liquid, and the second cavity 452 is used to receive the processed sample. The outer wall of the extraction box 410 is rotatably connected to a cylindrical gear 470, and a rack 460 that meshes with the cylindrical gear 470 is connected to the transfer box 450.

[0038] During the directional adsorption of HBV-DNA by the magnetic microspheres 481, the remaining waste liquid can drip into the first cavity 451 of the transfer box 450.

[0039] Multiple diversion tubes 432 are connected to the rectangular plate 430, and the multiple diversion tubes 432 are connected to the eluent pipeline 441. An L-shaped frame 440 is fixedly connected to the side of the rectangular plate 430, and a press valve 442 is installed on the L-shaped frame 440. The press valve 442 is installed in the eluent pipeline 441. A stop block 443 that cooperates with the press valve 442 is provided on the extraction box 410. When the transfer box 450 moves so that the second chamber 452 is directly below the rectangular plate 430, the transfer box 450 can push the rectangular plate 430 to slide through the L-shaped frame 440, so that the diversion tubes 432 are directly aligned with the magnetic microspheres 481, and the press valve 442 contacts the stop block 443, thereby opening the eluent pipeline 441. Both the first chamber 451 and the second chamber 452 are connected to a discharge pipe 453. The discharge pipe 453 on the second chamber 452 is connected to the fluorescence detection device.

[0040] After the sample is discharged, the cylindrical gear 470 is rotated. At this time, the cylindrical gear 470 drives the transfer box 450 to slide through the rack 460, so that the second chamber 452 can be located under the rectangular plate 430. After the transfer box 450 moves, the end of the transfer box 450 can push the L-shaped frame 440. At this time, the L-shaped frame 440 drives the rectangular plate 430 to slide, so that the diversion tube 432 at the bottom of the rectangular plate 430 can be directly facing the magnetic microspheres 481. At the same time, the pressing valve 442 contacts the stop block 443, so that the pressing valve 442 opens. At this time, the elution pipe 441 opens, and the elution flows to the magnetic microspheres 481 through the diversion tube 432. At the same time, the magnetic circuit of the magnetic microspheres 481 is closed, so that the extracted HBV-DNA is received by the second chamber 452. The discharge tube 453 on the second chamber 452 is connected to the fluorescence detection device, so that the HBV-DNA load can be detected. Negative pressure pumps can be installed on both discharge pipes 453 to ensure that samples and waste liquid can be discharged as completely as possible.

[0041] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A viral load detection device for HBV-related liver cancer research, comprising an organism (100), characterized in that: The body (100) is equipped with a sample processing module (200) and a nucleic acid extraction module (400). A filtering module (300) is connected between the sample processing module (200) and the nucleic acid extraction module (400). The filtering module (300) includes a sample dispensing tube (310) and a transfer tube (320). The transfer tube (320) is connected between the sample processing module (200) and the nucleic acid extraction module (400). The sample dispensing tube (310) is connected to the middle of the transfer tube (320). Both the sample loading tube (310) and the transfer tube (320) are equipped with one-way valves. The transfer tube (320) is equipped with a mounting shell (360). A filter screen (361) is rotatably connected inside the mounting shell (360). When the sample loading tube (310) adds lysis buffer to the sample processing module (200), the filter screen (361) does not rotate. When the sample in the sample processing module (200) is transferred to the nucleic acid extraction module (400) through the transfer tube (320), the filter screen (361) rotates to move and filter tissue residue.

2. The viral load detection device for HBV-related liver cancer research according to claim 1, characterized in that: The filtration module (300) also includes a dosing pump (330) connected to the transfer tube (320), and the sample processing module (200) is equipped with a motor (340) for driving the dosing pump (330).

3. The viral load detection device for HBV-related liver cancer research according to claim 2, characterized in that: The filter screen (361) is fixedly connected to the middle of the mounting shaft (353), and the end of the mounting shaft (353) is connected to the rotating shaft (350) through the one-way bearing (352). The end of the rotating shaft (350) is equipped with a driven bevel gear (351), and the output end of the motor (340) is equipped with the driving bevel gear (341) that meshes with the driven bevel gear (351). When the motor (340) rotates forward, the injection pump (330) draws external lysis buffer through the sample tube (310), and the rotating shaft (350) rotates relative to the mounting shaft (353). When the motor (340) rotates in reverse, the injection pump (330) draws the processed sample and transfers it to the nucleic acid extraction module (400) through the transfer tube (320), and the rotating shaft (350) drives the mounting shaft (353) to rotate synchronously.

4. The viral load detection device for HBV-related liver cancer research according to claim 1, characterized in that: The inner wall of the mounting housing (360) is fixedly connected to a scraper (362), which is in contact with the filter screen (361).

5. The viral load detection device for HBV-related liver cancer research according to claim 1, characterized in that: The sample processing module (200) includes a temperature control box (210), a tube rack (230) is placed inside the temperature control box (210), test tubes containing tissue samples are placed on the tube rack (230), and a protrusion (220) is provided on the inner wall of the temperature control box (210), which can support the tube rack (230).

6. The viral load detection device for HBV-related liver cancer research according to claim 5, characterized in that: The sample processing module (200) also includes a top plate (240), on which multiple small motors (251) are fixedly connected. Sterile grinding heads (250) are installed at the output ends of the multiple small motors (251). Multiple branch tubes (260) are connected to the top plate (240) near the multiple sterile grinding heads (250). The multiple branch tubes (260) are all connected to the sample dispensing tube (310). The up and down movement of the top plate (240) can drive the sterile grinding heads (250) and the branch tubes (260) to enter the corresponding test tubes simultaneously.

7. The viral load detection device for HBV-related liver cancer research according to claim 1, characterized in that: The nucleic acid extraction module (400) includes an extraction box (410), a cover plate (420) is installed on the top of the extraction box (410), a cavity communicating with the transfer tube (320) is opened inside the cover plate (420), a plurality of liquid outlet holes (421) communicating with the cavity are opened on the lower surface of the cover plate (420), and a plurality of magnetic microspheres (481) are fixedly installed at the lower part of the plurality of liquid outlet holes (421).

8. The viral load detection device for HBV-related liver cancer research according to claim 7, characterized in that: A rectangular plate (430) is fixedly connected inside the extraction box (410), and a fixing frame (480) is fixedly connected inside the extraction box (410). Multiple magnetic microspheres (481) are fixedly connected to the fixing frame (480). The rectangular plate (430) has conical holes (431) that correspond one-to-one with the multiple magnetic microspheres (481). After the sample drips through the liquid outlet (421), it can be guided to drip onto the magnetic microspheres (481) through the conical holes (431).

9. The viral load detection device for HBV-related liver cancer research according to claim 8, characterized in that: The extraction box (410) is slidably connected to the middle of a transfer box (450). The transfer box (450) has a first cavity (451) and a second cavity (452). The first cavity (451) is used to receive waste liquid, and the second cavity (452) is used to receive the processed sample. The outer wall of the extraction box (410) is rotatably connected to a cylindrical gear (470), and the transfer box (450) is connected to a rack (460) that meshes with the cylindrical gear (470).

10. The viral load detection device for HBV-related liver cancer research according to claim 9, characterized in that: Multiple diversion pipes (432) are connected to the rectangular plate (430), and the multiple diversion pipes (432) are connected to the eluent pipeline (441). An L-shaped frame (440) is fixedly connected to the side of the rectangular plate (430), and a press valve (442) is installed on the L-shaped frame (440). The press valve (442) is installed in the eluent pipeline (441), and the extraction box (410) is provided with a stop that cooperates with the press valve (442). When the transfer box (450) moves so that the second cavity (452) is directly below the rectangular plate (430), the transfer box (450) can push the rectangular plate (430) to slide through the L-shaped frame (440), so that the diversion tube (432) corresponds to the magnetic microsphere (481), and the pressing valve (442) contacts the stop block (443), so that the eluent pipeline (441) is opened; Both the first cavity (451) and the second cavity (452) are connected to a discharge pipe (453), and the discharge pipe (453) on the second cavity (452) is connected to a fluorescence detection device.

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