A strain of hankania hymenoptera in shrimp intestinal tract and its application in aquaculture

Abstract

The application discloses a shrimp intestinal tract Luperella hanseatica strain and application thereof in aquaculture, characterized in that the strain is an M81 strain, is classified and named as Luperella hanseatica M81, is preserved in the China General Microbiological Culture Collection Center on April 27, 2026, and has a preservation number of CGMCC No. 38461, the application of the shrimp intestinal tract Luperella hanseatica strain and a synthetic microbial population composed of the Luperella hanseatica strain and Paracoccus marcusii RBB26 in preparation of a Vibrio parahaemolyticus inhibitor and in preparation of a probiotic additive for shrimp culture, and the advantages are that the Luperella hanseatica strain has the abilities of inhibiting growth of Vibrio and producing a biofilm, and the synthetic microbial population can further improve the survival rate of shrimp culture and the anti-invasion ability against Vibrio parahaemolyticus.

Description

Technical Field

[0001] This invention relates to a strain of *Hanstiania rumeniana* and its applications, particularly to a strain of *Hanstiania rumeniana* from the intestinal tract of shrimp and the application of its constructed synthetic flora in aquaculture. Background Technology

[0002] Litopenaeus vannamei is one of the most important aquaculture species in my country, boasting advantages such as rapid growth, strong adaptability, high disease resistance, and high economic value. However, with the rapid development of intensive and high-density aquaculture, problems such as deterioration of the aquatic environment and frequent disease outbreaks have become increasingly prominent, severely restricting the green and sustainable development of the shrimp farming industry. Currently, disease control in production mainly relies on large-scale water exchanges and chemical drugs, but these methods not only increase farming costs but also easily cause environmental pollution and may disrupt the microecological balance of the aquaculture system, posing certain biosecurity risks. Therefore, finding green disease control products for aquatic animals is imperative.

[0003] Microbial preparations, due to their non-toxic, pollution-free, and significant effects, have become an important direction in aquatic animal health research. However, most existing commercial microbial preparations are derived from terrestrial environments or non-aquatic hosts, and their strains are difficult to stably colonize in aquaculture water and shrimp intestines, resulting in unstable function, insufficient specificity, and limited control effects. In contrast, native microorganisms derived from aquaculture systems or the host's gut typically have stronger environmental adaptability and ecological competitive advantages, showing better application potential in inhibiting pathogens, maintaining microecological balance, and promoting host health. Based on this, synthetic microbial community strategies based on local microbial resources have been further developed in recent years. Related studies have shown that by rationally combining different functional strains, metabolic complementarity and synergistic effects among the microbial communities can be achieved, thereby significantly enhancing the inhibitory ability against pathogenic microorganisms and demonstrating good application prospects in actual production environments.

[0004] *Hanstiania rhynchophylla* is a Gram-negative, yellow-orange, non-spore-forming, aerobic bacterium. Existing isolates are mainly from estuarine regions and possess several unique properties, including being daunorubicin-positive and producing carotenoid pigments such as zeaxanthin. Currently, there are no publicly available reports, either domestically or internationally, on the isolation of *Hanstiania rhynchophylla* from shrimp intestines and its application in aquaculture. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a strain of *Hanstiania lumbricoides* in the shrimp gut that has the ability to inhibit the growth of *Vibrio parahaemolyticus* and the production of biofilms, and can improve the survival rate of shrimp farming and the ability to resist *Vibrio parahaemolyticus* infection, and the application of the synthetic flora constructed therein in shrimp farming.

[0006] The technical solution adopted by this invention to solve the above-mentioned technical problem is: a strain of *Hanstiglerus hupehensis* from the intestine of shrimp, which is strain M81 and classified as *Hanstiglerus hupehensis* M81 (…). Hanstruepera sp.) M81, accession number CGMCC No.38461, accession date April 27, 2026, deposited at the China General Microbiological Culture Collection Center, located at No.3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, China, Institute of Microbiology, Chinese Academy of Sciences.

[0007] This invention also provides a mixed bacterial strain consisting of *Paragonimus westermani* RBB26 and *Hansgrohes rüpoensis* M81, wherein: the *Paragonimus westermani* ( Paracoccus homiensis RBB26, deposited on May 30, 2023 at the China General Microbiological Culture Collection Center (CGMCC), accession number CGMCC No. 27498; the described *Hanstigerella hansternii* (RBB26) Hanstruepera sp.) M81 was deposited on April 27, 2026 at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38461.

[0008] The present invention also provides the application of the above-mentioned *Hanstiania rumeniana* strain from the shrimp intestine and the synthetic bacterial community constructed therefrom in the preparation of *Vibrio parahaemolyticus* inhibitors.

[0009] This invention also provides the application of the above-mentioned *Hansgroheumatica* strain from the shrimp gut and its constructed synthetic flora in the preparation of probiotic additives for aquaculture. The concentration in the aquaculture water is 1×10⁻⁶. 5 ~1×10 9 CFU / mL.

[0010] Compared with the prior art, the advantages of the present invention are as follows: The present invention discloses a strain of *Hanstiania rumeniana* from the shrimp gut and the application of its constructed synthetic flora in shrimp farming. This strain was isolated from the shrimp gut, and its OD value after 24 h of culture was [missing information]. 600 The value reaches approximately 1.0; this strain also inhibits the growth of Vibrio parahaemolyticus and the biofilm activity of its products, effectively reducing the risk of pathogen colonization. Simultaneously, it can regulate the intestinal flora structure of shrimp, maintain the microecological balance of the aquaculture system, thereby improving the shrimp's immunity and disease resistance; in terms of metabolic regulation, this invention discovered that vitamin B... 12 It plays a key regulatory role in the growth of the strain, and exogenous vitamin B is added. 12 Or supplement with vitamin B 12 The culture supernatant of the strain significantly promoted the growth of M81, indicating its important role in microbial interactions and nutrient utilization. Based on this, the M81 strain was combined with a vitamin B-rich... 12A synthetic microbial community is formed by mixing *Paragonimus westermani* RBB26 in equal proportions, which can further enhance the synergistic effect among the microbial communities. Therefore, *Hanstiania rhynchophylla* M81 and its synthetic microbial community have significant economic value in aquaculture, especially in the development of novel green feed additives and antibiotic alternatives. Attached Figure Description

[0011] Figure 1 The pH and OD changes of *Hansgrohes hanseria müperii* M81 over culture time 600 Value change; Figure 2 Clustering tree constructed based on the 16S rDNA sequence of *Hansgrohes hanseria müperi* strain M81; Figure 3 A clustering tree constructed based on the genome sequence of *Hans rüperella* strain M81; Figure 4 The inhibitory effect of Hanstil Rüperkal M81 on Vibrio parahaemolyticus is shown, where A represents the inhibition of Vibrio parahaemolyticus growth and B represents the inhibition of Vibrio parahaemolyticus biofilm production. Figure 5 A represents different concentrations of vitamin B. 12 The growth-promoting effect of RBB26 culture supernatant on strain M81, B represents the growth-promoting effect of RBB26 culture supernatant on strain M81, and C represents the intracellular vitamin B of strain RBB26. 12 content; Figure 6 The effects of M81, RBB26, and the constructed synthetic bacterial community on shrimp growth performance are shown, where A represents survival rate, B represents yield, and C represents resistance to Vibrio infection. SynCom RM This indicates a synthetic bacterial community. Detailed Implementation

[0012] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments.

[0013] Example 1: Isolation, purification and identification of the strain.

[0014] 1. Isolation and purification of strains Based on previous research findings that *Hanstiglerus* significantly accumulates in the intestines of healthy shrimp, this study aimed to isolate it. Under sterile conditions, live shrimp intestines were picked up with a sterile toothpick and placed into sterile EP tubes. Autoclaved PBS buffer was added, and the tubes were vigorously shaken for 30 seconds before being incubated at 30°C and 180 rpm for 30 minutes. Then, the culture was transferred at a 2% (v / v) ratio to fresh 1 / 2 2216E broth and incubated at 30°C and 180 rpm for 24 hours. A suitable amount of the culture was diluted and evenly spread onto 1 / 2 2216E agar medium and incubated upside down at 30°C for 2–3 days. Single colonies were picked up with a sterile inoculation loop, purified twice on 2216E agar medium, and DNA was extracted and its 16S DNA was measured. rDNA was used for identification, and the strain identified as *Hansgrohes hansterleuproides* was preserved at -80℃. Through physiological and biochemical assays and culture experiments, strain M81 was selected as the invention strain. This strain is a Gram-negative, rod-shaped bacterium; its colonies are orange-yellow, semi-transparent, and raised, with flattened, round cells; the optimal growth temperature for this strain is 30℃. Figure 1 As shown, this strain was cultured in 2216E broth for 9 h, and the OD... 600 The pH value increased significantly, stabilized after 15 h, and at this time the pH value of the culture medium began to rise significantly.

[0015] The formulation of the 2216E broth medium used for isolation and purification culture was as follows: 5.0 g peptone, 1.0 g yeast extract, 0.1 g ferric citrate, 19.45 g sodium chloride, 5.98 g magnesium chloride, 3.24 g sodium sulfate, 1.8 g calcium chloride, 0.55 g potassium chloride, 0.16 g sodium carbonate, 0.08 g potassium bromide, 0.034 g strontium chloride, 0.022 g boric acid, 0.004 g sodium silicate, 0.0024 g sodium fluoride, 0.0016 g sodium nitrate, 0.008 g dipotassium hydrogen phosphate, 1000 mL distilled water, pH 7.4–7.8. The 2216E agar medium used was 2216E broth medium supplemented with 1.8% agar by weight of 2216E broth medium.

[0016] PCR amplification of the 16S rDNA gene: Genomic DNA was extracted from the screened strain M81 using a bacterial genomic DNA extraction kit from Tiangen Biotech Co., Ltd. PCR amplification was performed using the genomic DNA as a template. The primers used were: 27F: 5'-AGAGTTTGATCCTGGCTCAG-3'; 1492R: 5'-GGTTACCTTGTTACGACTT-3'.

[0017] The PCR reaction system consisted of (20 μl) of: 2 μl template, 10 μl kodaq2×PCR MasterMix (Cat. No. G497, abm, Canada), 0.5 μl primer 1 (27F) (10 μM), 0.5 μl primer 2 (1492R) (10 μM), and 7 μl ddH2O. The PCR reaction program was: 94℃ pre-denaturation for 3 min; 94℃ denaturation for 30 s, 58℃ annealing for 1 min, 72℃ extension for 30 s, 35 cycles; and 72℃ extension for 5 min. PCR was performed by electrophoresis on a 1.5 wt% agarose gel, and the band was approximately 1500 bp. The purified sample was then sent to Shanghai Sangon Biotech Co., Ltd. for sequencing, yielding a 16S rDNA sequence of 1431 bp, and a 16S rDNA sequence of 765 bp for strain M81. After submitting the M81 sequence to EZBioCloud for comparison, it was found that this strain is similar to the type strain. Hanstruepera neustonica The similarity to strain CC-PY-50 was only 96.04%, therefore, genome sequencing was performed on M81. The 16S rDNA clustering tree of this strain is attached. Figure 2 .

[0018] 2. Genome sequencing and identification of the strain of this invention Genome sequencing of M81: Total DNA was extracted using the EZNA Bacteria DNA kit (OMEGA) according to the manufacturer's instructions. DNA concentration and quality were measured using a Nanodrop 2000 spectrophotometer (NanoDrop Technologies, DE, USA), and the DNA was stored at -20°C for further analysis. Samples were sequenced on an Illumina NovaSeq 6000 platform using 150 bp paired-end reads. Sequencing libraries were constructed using the Illumina TruSeq™ Nano DNA Sample Prep Kit method. Average Nucleotide Identity (ANI) was used to compare strain M81 with publicly available DNA samples in the NCBI database. Hanstruepera Comparative analysis with relevant reference genomes was conducted. The results showed that the ANI (Average Nomenclature Indicator) values ​​of strain M81 with all reference genomes ranged from 79.2% to 80.9%, significantly lower than the generally accepted species classification threshold of 95%. This result indicates that strain M81 is comparable to known... Hanstruepera All species in this genus exhibit significant genomic differentiation, do not belong to any previously reported species, and possess clear taxonomic characteristics of a novel species. Therefore, this strain M81 was named... Hanstrueperasp. M81 was deposited on April 27, 2026, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38461, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. The genome clustering tree of this strain is attached. Figure 3 .

[0019] 3. Determination of the ability of the strain of this invention to inhibit the growth of Vibrio parahaemolyticus and produce biofilms. Inhibition of Vibrio parahaemolyticus growth: After activation, the isolated strain was cultured in 2216E broth at 30°C for 48 h. After culture, the bacterial cells were removed by centrifugation at 8,000 rpm for 5 min, and the supernatant was collected and sterilized by filtration through a 0.22 μm polyethersulfone (Millipore) membrane. The sterile supernatant was stored at -20°C for later use. Vibrio parahaemolyticus was cultured overnight in 2216E broth at 30°C. For the experiment, the sterile supernatant was added to 2216E broth at a ratio of 30% (v / v) to prepare a mixed culture medium (CFS), and inoculated with a washed Vibrio parahaemolyticus suspension to achieve initial OD... 600 The value was 0.01. The OD was measured during the cultivation process. 600 The growth of the strain was monitored, and the results were as follows: Figure 4 As shown in A in the figure. Compared with the control group 2216E broth medium (denoted as CK), adding M81 sterile supernatant at a ratio of 30% (v / v) to the corresponding culture system can significantly inhibit the growth of Vibrio parahaemolyticus.

[0020] Determination of Vibrio parahaemolyticus biofilm inhibition: 2216E broth was used as the control group (CK), and a mixed medium containing 30% sterile supernatant was used as the experimental group (CFS). 100 μL of medium was added to each well of a sterile 96-well polystyrene microplate (Corning, USA). Subsequently, 10 μL of Vibrio parahaemolyticus bacterial suspension (OD200) was inoculated. 600Adjust to step 1), incubate at 30℃ for 48 hours, and seal with sealing film to prevent liquid evaporation. After incubation, aspirate the culture medium, add 200 μL of sterile PBS buffer to each well to wash the wells three times, then add 100 μL of methanol to each well for 15 min to fix, aspirate the methanol from the wells, and allow to air dry. Next, add 100 μL of 1% crystal violet solution to each well and stain at room temperature for 5 min. Aspirate the crystal violet staining solution from the wells and rinse off excess dye with running water. Invert the culture plate on filter paper to remove residual water and dry in a 37℃ oven. After complete drying, add 100 μL of 33% glacial acetic acid solution to each well and incubate at 37℃ for 30 min to dissolve the crystal violet. Measure the OD value of the solution in the wells at 590 nm using a microplate reader. Perform a replicate of 3 wells for each strain, and take the average of the 3 values ​​(OD value). The results are shown below. Figure 4 As shown in B in the diagram. (By...) Figure 4 As shown in B, adding 30% of sterile supernatant of M81 (v / v) can inhibit the formation of Vibrio biofilm. The above results indicate that M81 may exert its effects by secreting antibacterial metabolites or interfering with the quorum sensing system.

[0021] Example 2: Complementary effect between strain M81 and strain RBB26.

[0022] 1. Vitamin B 12 M81 growth promoters were added: The strain was initially activated and cultured in 2216E broth, and then transferred to ASW medium for subsequent experiments. The strain was incubated at 30°C with shaking at 180 rpm. To minimize interference from vitamins and related metabolites carried by the cells during inoculation, bacterial cells were collected by centrifugation at 8,000 rpm for 5 min before inoculation, washed three times with vitamin-free ASW medium, resuspended, and inoculated into ASW medium to prepare the M81 inoculum. The initial optical density (OD) was adjusted. 600 The value was 0.01. During the cultivation process, the optical density at a wavelength of 600 nm was measured to monitor the growth of the strain and to plot a growth curve. The results are as follows: Figure 5 As shown in A, the strain of this invention is vitamin B. 12 Atrophic strains, whose growth is affected by vitamin B 12 Regulation.

[0023] The artificial seawater culture medium (ASW) formula is as follows: sodium sulfate 4.0 g, potassium dihydrogen phosphate 0.2 g, ammonium chloride 0.25 g, sodium chloride 20.0 g, magnesium chloride hexahydrate 3.0 g, potassium chloride 0.5 g, calcium chloride dihydrate 0.15 g, sodium bicarbonate 0.19 g, and Milli-Q ultrapure water 1000 mL (resistivity <18.2 MΩ·cm); additionally added are glucose 2 mM, glutamate 2 mM, sodium acetate 5 mM, and trace element solution 1 mL / L, supplemented with vitamins B1, B2, B3, B5, B6, and B7, each with a final concentration of 400 pM; vitamin B... 12 The addition amounts were 0 pM, 10 pM, 25 pM, 50 pM, and 100 pM, respectively. The trace element solution formula was as follows: per liter, it contained 2.1 g of ferrous sulfate heptahydrate, 13.0 mL of 25% hydrochloric acid, 5.2 g of disodium ethylenediaminetetraacetate (Na2-EDTA), 30.0 mg of boric acid, 100.0 mg of manganese chloride tetrahydrate, 190.0 mg of cobalt chloride hexahydrate, 24.0 mg of nickel chloride hexahydrate, 2.0 mg of copper chloride dihydrate, 144.0 mg of zinc sulfate heptahydrate, and 36.0 mg of sodium molybdate dihydrate.

[0024] 2. Vitamin B 12 Experiment on the culture of supernatant of producing bacteria: It contains vitamin B 12 The strain with synthetic ability was *Paragonimus westermani* RBB26, previously deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 27498. Single colonies of RBB26 were picked and inoculated into 2216E broth, cultured overnight at 30°C with shaking at 180 rpm, and a vitamin-free 2216E bacterial suspension was prepared using sterile ASW medium following the same method as described above for the M81 inoculum.

[0025] Supernatants for each strain were prepared in 1 L conical shake flasks, with 300 mL of the corresponding culture medium added to each flask. The flasks were then incubated with shaking at 30°C and 180 rpm. After incubation, the bacterial cells were removed by centrifugation at 8,000 rpm for 5 min, and the supernatant was collected and sterilized by filtration through a 0.22 μm polyethersulfone (Millipore) membrane. The sterile supernatant was stored at -20°C for later use. During experiments, sterile RBB26 supernatant was added to the corresponding culture system at a ratio of 30% (v / v) to prepare a mixed culture medium, which was then inoculated with washed M81 bacterial suspension to achieve initial OD... 600 The value was 0.01. During the cultivation process, the growth of the strain was monitored by measuring OD600, and the results were as follows: Figure 5 As shown in B in the figure. The results indicate that, compared with the control group without added supernatant, the addition of RBB26 sterile supernatant significantly restored M81 in vitamin B deficiency.12 The growth capacity under certain conditions suggests that RBB26 may exert its growth through the secretion of vitamin B. 12 Or related metabolites are supplied to M81 to restore growth.

[0026] 3. Intracellular vitamin B in *Paracococcus faecium* strain RBB26 12 Content determination: After culturing *Paragonimus westermani* RBB26 in ASW medium for 48 h, the bacterial culture was collected. The cells were collected by centrifugation at 8,000 rpm for 10 min at 4 °C, followed by washing twice with PBS buffer to thoroughly remove extracellular metabolites. The washed cells were resuspended in lysis buffer (50 mM Tris-HCl, 1 mM EDTA, 1% Triton X-100, and a mixture of protease inhibitors) and incubated on ice for 30 min, with shaking every 5 min during incubation. The supernatant was then collected by centrifugation at 12,000 rpm for 15 min at 4 °C. VB 12 The content uses microbial vitamin B produced by Wuhan Gilead Biotechnology Co., Ltd. 12 (VB) 12 The enzyme-linked immunosorbent assay (ELISA) kit was used for quantitative analysis according to the instructions. The results are as follows: Figure 5 As shown in C, the Control refers to the lysate without inoculated bacterial cells, used to exclude the influence of reagent interference on the detection results. The concentration calculation formula is fitted using a four-parameter logistic model (4PL). The calculated concentration is corrected according to the dilution factor during sample pretreatment to obtain the vitamin B content in the sample. 12 The actual content.

[0027] Example 3: The effects of M81, RBB26, and the constructed synthetic microbial community on shrimp farming.

[0028] This experiment was conducted in a 5 L shrimp farming ecological experimental tank. Four treatment groups were set up: control group (CK), *Hanstiania rüperella* M81 group, *Paragonimus westermani* RBB26 group, and synthetic bacteria group (SynCom). RM M81 and RBB26 were mixed in a 1:1 ratio (to maintain a consistent total concentration of the synthetic bacterial community), and each group had 5 replicate culture tanks. Each treatment group was stocked with 200 P10 stage juvenile shrimp per tank. Shrimp larvae (P10 larvae) were also stocked with 200 larvae per experimental tank. Activation and addition of the bacterial agent: The preserved strain was activated by streaking on 2216E agar medium. After single colonies grew, a single colony was picked and inoculated into fresh 2216E broth medium and cultured overnight. The OD value was adjusted to 1.0, and the culture water was added at a concentration of 1×10⁻⁶. 7CFU / mL. In the treatment group, 0.1 mL of bacterial solution per gram of feed was added to 9.9 mL of sterilized seawater and soaked for 10–40 min before feeding. In the control group, 10 mL of sterilized seawater was used per gram of feed. The daily feed amount was 4% of body weight, divided into three feedings: 7:00 AM, 12:00 PM, and 5:00 PM. The experiment lasted for 6 days. After the experiment, the survival rate of shrimp and intestinal fullness were measured. After 6 days of culture, juvenile shrimp were transferred to 500 mL Vibrio infection bottles, with 15 juvenile shrimp in each bottle. The control group and experimental group each had uninfected and infected groups. Each uninfected group had 8 replicates, and each infected group had 12 replicates. Vibrio was added by culturing Vibrio parahaemolyticus in 2216E broth medium to an OD concentration of [missing value]. 600 When the absorbance is approximately 1.0, it is added to the culture bottle at a concentration of 1×10⁻⁶. 7 CFU / mL, the control group was replaced with sterile culture medium. Experimental results are as follows: Figure 6 As shown in Figures A and B, compared with the control group, the addition of single-strain M81 or RBB26 can improve shrimp survival rate and intestinal fullness to some extent; among them, the survival rate and intestinal fullness of the M81 treatment group increased by 6.4% and 9.1%, respectively. The synthetic microbial community treatment showed the most significant effect, with survival rate and intestinal fullness increasing by 9.2%–15.6% and 8.2%–17.4% compared with the control group, respectively. Figure 6 As shown in C, under Vibrio infection conditions, all probiotic treatments improved the shrimp's resistance to infection. Among them, the survival rate of juvenile shrimp in the M81 treatment group increased by 26.7% compared with the control group, while the synthetic bacteria treatment group increased by 40.7%, which was significantly better than the effect of single bacteria treatment.

[0029] The foregoing description is not intended to limit the invention, nor is the invention limited to the examples given. Any changes, modifications, additions, or substitutions made by those skilled in the art within the scope of the invention should also be considered within the protection scope of the invention.

Claims

1. A strain of *Hanstiania leuproides* from the intestinal tract of shrimp, characterized in that: This strain is M81 and is classified as *Hanstiania rumeniana*. Hanstruepera sp.) M81 was deposited on April 27, 2026 at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38461.

2. A synthetic microbial community, characterized in that: The synthetic bacterial community is a mixed strain of *Paragonimus westermani* RBB26 and *Hanstiania rumperi* M81, wherein *Paragonimus westermani* ( Paracoccus homiensis RBB26, deposited on May 30, 2023 at the China General Microbiological Culture Collection Center (CGMCC), accession number CGMCC No. 27498; the described *Hanstigerella hansternii* (RBB26) Hanstruepera sp.) M81 was deposited on April 27, 2026 at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38461.

3. The synthetic microbial community according to claim 2, characterized in that: The mixing ratio of the strains of *Paracoccus faecium* RBB26 and *Hanstiania rubella* M81 is 2:(1-3).

4. The use of the *Hanstiania rumeniana* strain of shrimp as described in claim 1 or the synthetic flora as described in claim 2 in the preparation of *Vibrio parahaemolyticus* inhibitors.

5. The application of the *Hansgroheumatica* strain of shrimp as described in claim 1 or the synthetic flora as described in claim 2 in the preparation of probiotic additives for aquaculture.

6. The application according to claim 5, characterized in that: The concentration of the *Hansgrohes hansterleuproides* strain or synthetic flora in the aquaculture water is 1 × 10⁻⁶. 5 ~1×10 9 CFU / mL.

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