A method for chemoenzymatic synthesis of a sulfur lactamycin and an enzyme catalysis system

By synthesizing thiolactam via a chemical enzymatic method, combining chemical and biological enzymatic methods, and using a hybrid enzyme catalytic system, the complex and costly synthesis of thiolactam in existing technologies has been solved, achieving efficient and environmentally friendly thiolactam synthesis.

CN122428002APending Publication Date: 2026-07-21SHANDONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG UNIV
Filing Date
2025-01-21
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

Existing methods for synthesizing thiolactamase are complex, costly, and have unsatisfactory yields, making it difficult to achieve efficient and environmentally friendly synthesis.

Method used

Thiolactam was synthesized using a chemical enzymatic method. (S)-2,4,6-trimethyl-3-oxooctyl-4,6-dienoic acid was synthesized chemically, and then thiolactam was synthesized using a biological enzymatic method. The in vitro one-step enzymatic reaction was carried out using a hybrid enzyme catalytic system consisting of bifunctional enzymes TlnCACP-Cy, TlnDA-PCP, and cytochrome P450 monooxygenase TlnA.

Benefits of technology

The stable and efficient synthesis of thiolactamase has been achieved, with high catalytic efficiency, mild reaction conditions, low cost, and environmental friendliness, showing good application prospects.

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Abstract

The present application relates to the field of chemical synthesis and biological catalysis, and relates to a chemical enzymatic synthesis method of a sulfur lactone and an enzyme catalysis system. (S)-2,4,6-trimethyl-3-oxo-oct-4,6-dienoic acid is first synthesized by a chemical method; then, the (S)-2,4,6-trimethyl-3-oxo-oct-4,6-dienoic acid is connected with coenzyme A by using coenzyme A ligase PbACL891 to form (S)-2,4,6-trimethyl-3-oxo-oct-4,6-dienoic acid-S-CoA; finally, a hybrid catalysis system composed of polyketide synthase (PKS)-non-ribosomal polypeptide synthesis enzyme (NRPS) TlnC ACP‑Cy , NRPS TlnD A‑PCP and cytochrome P450 monooxygenase TlnA is used to efficiently synthesize the sulfur lactone under the driving of an electron donor and a reductase partner protein. The method for preparing the sulfur lactone has the characteristics of high yield, low cost, environmental protection and the like, and has high industrial application potential.
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