KASP molecular marker combination related to tomato gray mold resistance and application thereof

By developing a combination of KASP molecular markers and primers related to resistance to tomato gray mold, and combining them with fluorescence signal determination, disease-resistant plants were successfully identified and screened. This solved the problem of slow breeding process in traditional methods and achieved efficient screening and breeding of disease-resistant varieties.

CN122428052APending Publication Date: 2026-07-21HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Filing Date
2026-04-17
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

Existing technologies are insufficient for effectively screening and breeding varieties resistant to tomato gray mold, traditional chemical control methods are ineffective, and the breeding process is slow.

Method used

We developed a combination of KASP molecular markers associated with resistance to tomato gray mold, used KASP primers for PCR amplification, and combined FAM and HEX fluorescence signals to determine plant genotypes. We also provided kits for identification and screening.

Benefits of technology

It enables accurate identification and rapid screening of resistance to tomato gray mold, with a detection accuracy rate of 81% to 89%, which accelerates the breeding process and saves time and costs.

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Abstract

The application carries out whole genome association analysis on 304 tomato natural population materials and resistance to gray mold, identifies 1 SNP site significantly related to resistance to gray mold on the long arm of chromosome 1 and 6 of tomato respectively, and identifies 2 SNP sites significantly related on the long arm of chromosome 3 of tomato. A set of KASP molecular markers Sl_ch01.85724661, Sl_ch03.137202, Sl_ch03.53604711 and Sl_ch06.40877867 are developed for screening tomato materials. The KASP molecular markers provided by the application provide new sites and new markers for tomato resistance to gray mold breeding; meanwhile, the KASP molecular markers can quickly screen tomato germplasm containing the site, effectively save the time cost, accelerate the detection of the genetic background of the tomato resistance germplasm, and have important significance for the breeding of tomato varieties resistant to gray mold.
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Citation Information

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