Application of nematode high-throughput screening technology in ginseng and astragalus health product development system and method

Abstract

The application discloses a ginseng health product development system and method using a nematode high-throughput screening technology, comprising a nematode standardization culture and pretreatment module, a ginseng sample pretreatment and gradient preparation module, a nematode high-throughput exposure and administration module, a multi-dimensional efficacy index real-time collection module, an efficacy data analysis and comprehensive evaluation module, a ginseng product formula optimization iteration module, a new product standardization and batch efficacy confirmation module; the application systematically applies the Caenorhabditis elegans high-throughput screening technology to the whole process development of the ginseng health product, constructs an in-vivo screening system at the tissue and organ level, breaks through the limitations of traditional cell experiments and mammalian models, and realizes the innovation of the efficacy evaluation mode. Relying on the nematode high-throughput screening characteristics, parallel screening of multiple formulas, multiple doses and multiple repetitions is realized, the efficacy evaluation period is shortened, the development cost is reduced, and the product development efficiency is improved.

Description

Technical Field

[0001] This invention relates to the fields of agricultural product quality and safety traceability and efficient fine-tuning of large model parameters, specifically to a system and method for developing ginseng and astragalus health products using high-throughput screening technology for nematodes. Background Technology

[0002] Agricultural product quality and safety are directly related to people's livelihood and industrial development. Full life-cycle traceability is a core means of achieving agricultural product quality supervision, responsibility determination, and consumer trust. Currently, agricultural product traceability systems generally suffer from technical bottlenecks such as heterogeneous multi-source data, rigid verification rules, insufficient intelligence, and difficulties in cost control. Specifically, these bottlenecks manifest in the following ways: Data formats in planting, processing, logistics, testing, and distribution are heterogeneous and standards are not uniform. Data cleaning and integration are costly and prone to problems such as missing, tampering, and inconsistency. Traditional systems struggle to achieve effective integration and standardized processing of multi-source data. General large models have problems such as semantic understanding bias and inaccurate knowledge matching in the field of agricultural product traceability. Full parameter fine-tuning methods consume a lot of computing power and have high deployment costs, which are difficult for small and medium-sized agricultural entities to bear. There is a lack of cost-effective fine-tuning mechanisms for agricultural scenarios. Existing source tracing and verification methods rely heavily on preset rules and manual review, which can only achieve single-dimensional verification and cannot integrate text semantics, knowledge association, and temporal logic for multi-dimensional reasoning. They also lack the ability to identify hidden anomalies. Most systems have not established a closed-loop mechanism for verification results and model optimization, so the model cannot continuously iterate based on real traceability data, making it difficult to improve long-term adaptability and verification accuracy. Existing technologies do not incorporate cost-effective help-based fine-tuning strategies, making it impossible to achieve rapid model adaptation and accuracy improvement under low sample and low computing power conditions, and thus failing to meet the lightweight deployment requirements of agricultural scenarios. Summary of the Invention

[0003] The purpose of this invention is to provide a system and method for developing ginseng and astragalus health products using high-throughput screening technology for nematodes, in order to solve the problems existing in the prior art.

[0004] To achieve the above objectives, the present invention provides the following technical solution: a ginseng and astragalus health product development system using nematode high-throughput screening technology, comprising a nematode standardized culture and pretreatment module, a ginseng and astragalus sample pretreatment and gradient formulation module, a nematode high-throughput exposure drug delivery module, a multi-dimensional efficacy index real-time acquisition module, an efficacy data analysis and comprehensive evaluation module, a ginseng and astragalus product formula optimization and iteration module, and a new product finalization and batch efficacy verification module. The standardized nematode culture and pretreatment module includes a synchronized hatching unit, a constant temperature culture unit, an instar sorting unit, and a sterile treatment unit, which are used to realize synchronized nematode culture, precise instar control, and sterile pretreatment. The ginseng and astragalus sample pretreatment and gradient preparation module includes a raw material extraction unit, a purification and impurity removal unit, a concentration and volume adjustment unit, and a gradient concentration preparation unit, which are used to complete the extraction, purification and gradient concentration preparation of ginseng and astragalus samples. The nematode high-throughput exposure drug delivery module includes a high-throughput sample loading unit, an exposure environment control unit, a drug delivery unit, and a sealed light-proof unit, which is used to achieve high-throughput controllable exposure of nematodes and ginseng and astragalus samples. The multi-dimensional efficacy indicator real-time acquisition module includes a lifespan indicator acquisition unit, an antioxidant indicator acquisition unit, an immune indicator acquisition unit, and a motor ability acquisition unit, which are used to collect phenotypic data related to anti-aging, antioxidant, and immune regulation in nematodes. The efficacy data analysis and comprehensive evaluation module includes a data noise reduction unit, a data normalization unit, an efficacy feature extraction unit, and a comprehensive score calculation unit, which are used to complete efficacy data processing and comprehensive scoring. The formulation optimization and iteration module for ginseng and astragalus products includes a ratio optimization unit, an excipient screening unit, a dosage form adaptation unit, and a formulation iteration verification unit, which are used to complete formulation optimization and iteration based on efficacy results. The new product finalization and batch efficacy verification module includes a product preparation unit, a pilot-scale verification unit, a batch consistency testing unit, and an efficacy verification unit, which are used to complete product finalization, process verification, and efficacy verification.

[0005] Furthermore, the synchronized hatching unit of the nematode standardized culture and pretreatment module uses a combination of alkaline digestion and centrifugal purification to obtain synchronized nematode eggs; the isothermal culture unit maintains constant culture environment parameters; the instar sorting unit completes specific instar sorting based on nematode body size and movement characteristics; and the aseptic treatment unit removes bacteria from the nematode body surface and body.

[0006] Furthermore, the raw material extraction unit of the ginseng and astragalus sample pretreatment and gradient preparation module is used to enrich the polysaccharides, saponins, and flavonoids in the ginseng and astragalus raw materials; the purification and impurity removal unit is used to remove non-functional impurities; the concentration and volume adjustment unit is used to prepare standard mother liquor; and the gradient concentration preparation unit is used to prepare multiple sets of gradient concentration samples by stepwise dilution.

[0007] Furthermore, the multi-dimensional efficacy indicator real-time acquisition module is configured with a nematode average lifespan extension rate calculation model, and the calculation formula is as follows: In the formula: The average lifespan extension rate of nematodes; The average lifespan of nematodes in the Astragalus and Ginseng sample treatment group; The average lifespan of nematodes in the blank control group is shown.

[0008] Furthermore, the efficacy data analysis and comprehensive evaluation module is configured with an antioxidant activity comprehensive scoring calculation model, and the calculation formula is as follows: In the formula: The overall score is based on antioxidant activity. The number of antioxidant evaluation indicators; For the first Weighting coefficients for antioxidant indicators; For the first Normalized values ​​of antioxidant indicators.

[0009] Furthermore, the efficacy data analysis and comprehensive evaluation module is configured with an immunity enhancement index calculation model, the calculation formula of which is: In the formula: An index for enhancing immunity; The survival rate of nematodes under stress in the ginseng and astragalus sample treatment group; The stress survival rate of nematodes in the blank control group; This is the system error correction coefficient.

[0010] Furthermore, the formulation optimization and iteration module of the ginseng and astragalus product is guided by the efficacy evaluation results, and completes the adjustment of the ratio of ginseng and astragalus, screening of excipients, adaptation of dosage form and closed-loop iterative verification of the formulation.

[0011] Furthermore, the new product finalization and batch efficacy verification module uses nematode high-throughput screening technology to complete the efficacy retesting of finished product batches, ensuring the consistency of efficacy across multiple batches of products.

[0012] The development method of a ginseng and astragalus health product development system using nematode high-throughput screening technology includes the following steps: Step 1: Start the standardized nematode culture and pretreatment module to prepare standardized sterile nematode samples; Step 2: Start the ginseng and astragalus sample pretreatment and gradient preparation module to complete the extraction of ginseng and astragalus samples and the preparation of gradient concentrations; Step 3: Activate the nematode high-throughput exposure drug delivery module to complete the high-throughput exposure treatment of nematodes and ginseng and astragalus samples; Step 4: Activate the multi-dimensional efficacy indicator real-time acquisition module to collect multi-dimensional efficacy data and calculate the average life extension rate of nematodes; Step 5: Activate the efficacy data analysis and comprehensive evaluation module to complete data processing and calculate the comprehensive score of antioxidant activity and the immunity enhancement index; Step 6: Activate the formulation optimization and iteration module for ginseng and astragalus products, and complete formulation optimization and iteration verification based on efficacy results; Step 7: Activate the new product finalization and batch efficacy verification module to complete product preparation, pilot testing, batch testing and efficacy verification; Step 8: Archive all development process data in the system to form a product development database.

[0013] Compared with the prior art, the beneficial effects of the present invention are: This invention systematically applies high-throughput screening technology of *C. elegans* to the entire development process of ginseng and astragalus health products, constructing an in vivo screening system at the tissue and organ level. This overcomes the limitations of traditional cell experiments and mammalian models, revolutionizing efficacy evaluation methods. Leveraging the high-throughput screening characteristics of nematodes, parallel screening with multiple formulations, dosages, and replicates is achieved, shortening the efficacy evaluation cycle, reducing development costs, and improving product development efficiency. Using live nematodes as the evaluation model, in vivo physiological responses are simulated, and multi-dimensional indicators of anti-aging, anti-oxidation, and immune enhancement are collected. Combined with a quantitative scoring model, this achieves standardized, objective, and precise efficacy evaluation. Formula iteration is completed based on precise efficacy data, moving away from traditional experience-based optimization methods and achieving scientific and data-driven approaches to ginseng and astragalus ratios, excipient selection, and dosage form adaptation, ensuring optimal product efficacy. Batch consistency testing and nematode efficacy retesting ensure the stability of product components and efficacy across multiple batches, improving product quality control and market competitiveness. Attached Figure Description

[0014] Figure 1 This is a system module diagram of the present invention; Figure 2 This is a schematic diagram of the nematode standardized culture and pretreatment module of the present invention; Figure 3 This is a schematic diagram of the sample pretreatment and gradient preparation module of Astragalus membranaceus of the present invention; Figure 4 This is a schematic diagram of the nematode high-throughput exposure drug delivery module of the present invention; Figure 5 This is a schematic diagram of the real-time acquisition module for multi-dimensional efficacy indicators of the present invention. Figure 6 This is a flowchart of the method of the present invention. Detailed Implementation

[0015] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0016] Please see Figure 1-6 This invention provides a system and method for developing ginseng and astragalus health products using high-throughput screening technology for nematodes, including a standardized nematode culture and pretreatment module, a ginseng and astragalus sample pretreatment and gradient preparation module, a nematode high-throughput exposure drug delivery module, a multi-dimensional efficacy index real-time acquisition module, an efficacy data analysis and comprehensive evaluation module, a ginseng and astragalus product formula optimization and iteration module, and a new product finalization and batch efficacy verification module. The standardized nematode culture and pretreatment module is used to achieve synchronized culture, precise control of instars, constant regulation of environmental parameters, and aseptic pretreatment of *C. elegans*, eliminating individual differences and environmental interference and ensuring the consistency of screened samples. It includes a synchronized hatching unit, a constant-temperature culture unit, an instar sorting unit, and an aseptic treatment unit. Synchronized hatching unit: The method of combining alkaline digestion and centrifugal purification is used to obtain synchronized nematode eggs, eliminate differences in developmental sequence, and ensure that all nematodes start in the same physiological state. Constant temperature culture unit: Provides a constant culture environment, maintains stable parameters such as culture medium composition, temperature, and humidity, and provides standardized conditions for nematode growth; Instar sorting unit: Based on the body size and movement characteristics of nematodes, complete the precise sorting of nematodes of specific instars, remove immature and senescent individuals, and ensure that the physiological stages of the nematodes used for screening are consistent; Aseptic processing unit: Removes bacteria from the surface and inside the nematodes to avoid microbial interference with efficacy evaluation results and ensure the sterility of the screening environment.

[0017] The Astragalus and Ginseng Sample Pretreatment and Gradient Preparation Module is used to complete the extraction, purification, concentration, and standardized preparation of gradient concentration samples from Astragalus and Ginseng raw materials, ensuring uniform sample composition and accurate concentration, and adapting to high-throughput screening requirements. It includes a raw material extraction unit, a purification and impurity removal unit, a concentration and volume adjustment unit, and a gradient concentration preparation unit. Raw material extraction unit: Targeted extraction of Codonopsis pilosula and Astragalus membranaceus raw materials, enriching polysaccharides, saponins, flavonoids and other functional components, and preserving the natural activity of the raw materials; Purification and impurity removal unit: Removes impurities such as starch, cellulose, and pigments from the extract, reduces the interference of non-functional components on the screening results, and improves sample purity; Concentration and volume adjustment unit: Concentrates the purified extract to the target concentration, and then adjusts the volume to obtain a standard mother liquor, ensuring the stability of the mother liquor component concentration; Gradient concentration preparation unit: Based on the standard stock solution, multiple groups of gradient concentration samples are prepared by progressively diluting the stock solution to achieve parallel screening of different dose groups.

[0018] The nematode high-throughput exposure and drug delivery module enables high-throughput parallel drug delivery in multi-well plates, precise control of the exposure environment, and standardized drug dosage. It allows for controlled exposure of nematode and ginseng / astragalus samples, ensuring consistent screening throughput and operational procedures. The module includes a high-throughput sample loading unit, an exposure environment control unit, a drug delivery unit, and a sealed, light-protected unit. High-throughput sample loading unit: Adapted to multi-well plate carriers, enabling parallel loading of multiple sets of samples and multiple repetitions to meet the batch processing requirements of high-throughput screening; Exposure environment control unit: Maintains constant environmental parameters during exposure to ensure that the exposure conditions of nematodes in different groups are completely consistent; Dosing unit: Precisely controls the dosage volume and contact method of ginseng and astragalus samples to ensure uniform and stable exposure dose for each nematode; Sealed and light-proof unit: The exposed system is sealed and protected from light to prevent sample volatilization, photodegradation and external contamination, and to maintain the stability of the exposed system.

[0019] The multi-dimensional efficacy indicator real-time acquisition module is used to collect phenotypic indicators related to anti-aging, anti-oxidation, and enhanced immunity in nematodes after treatment with ginseng and astragalus, providing raw data for subsequent efficacy evaluation. It includes units for lifespan indicators, antioxidant indicators, immune indicators, and motor function. Lifespan index acquisition unit: continuously monitors the survival status of nematodes, records survival time and death time, and calculates average lifespan and maximum lifespan; Antioxidant index acquisition unit: detects reactive oxygen species levels and antioxidant enzyme activity-related signals in nematodes to quantify the degree of oxidative stress response; Immune index collection unit: monitors the survival status of nematodes under stress conditions, reflecting immune regulation and stress resistance; Motion capacity acquisition unit: Records parameters such as the movement frequency and swing amplitude of nematodes, reflecting the body's physiological functions and aging degree.

[0020] This module incorporates a formula for calculating the average lifespan extension rate of nematodes, used to quantify anti-aging efficacy: In the formula: The average lifespan extension rate of nematodes; The average lifespan of nematodes in the Astragalus and Ginseng sample treatment group; The average lifespan of nematodes in the blank control group is shown.

[0021] The efficacy data analysis and comprehensive evaluation module is used to denoise, normalize, extract features, and comprehensively score the raw collected data, establish a multi-dimensional efficacy evaluation model, and output objective efficacy scores for ginseng and astragalus samples. It includes a data denoising unit, a data normalization unit, an efficacy feature extraction unit, and a comprehensive score calculation unit. Data noise reduction unit: Removes outliers and interference data, smooths out fluctuations in the original data, and improves data reliability; Data normalization unit: converts indicator data of different dimensions and magnitudes into values ​​within a unified range, eliminating differences in dimensions; Efficacy Feature Extraction Unit: Extracts core features related to anti-aging, anti-oxidation, and immune enhancement, and constructs an efficacy feature matrix; Comprehensive scoring calculation unit: Based on normalized data and feature matrix, calculate the comprehensive antioxidant score and immunity enhancement index to determine the efficacy level.

[0022] This module embeds a comprehensive scoring formula for antioxidant activity, used to quantify antioxidant efficacy: In the formula: The overall score is based on antioxidant activity. The number of antioxidant evaluation indicators; For the first Weighting coefficients for antioxidant indicators; For the first Normalized values ​​of antioxidant indicators.

[0023] It also incorporates an immune enhancement index calculation formula to quantify immune regulation efficacy: In the formula: An index for enhancing immunity; The survival rate of nematodes under stress in the ginseng and astragalus sample treatment group; The stress survival rate of nematodes in the blank control group; This is the system error correction coefficient.

[0024] The Codonopsis and Astragalus product formulation optimization and iteration module is used to optimize the ratio of Codonopsis and Astragalus based on efficacy evaluation results, screen excipients, adapt dosage forms, and iterate the formulation to form the optimal formulation. It includes a ratio optimization unit, an excipient screening unit, a dosage form adaptation unit, and a formulation iteration verification unit. Formula optimization unit: Based on efficacy scores, adjust the quality ratio of Codonopsis pilosula and Astragalus membranaceus to find the optimal ratio of raw materials for efficacy; Excipient screening unit: Combining efficacy data and dosage form requirements, screening suitable excipients to ensure that excipients do not interfere with the core efficacy; Dosage form adaptation unit: Based on the optimal formulation characteristics, it matches target dosage forms such as oral liquids and beverages, and determines the dosage form processing parameters; Formula Iteration Validation Unit: The optimized formula is re-validated for high-throughput efficacy against nematodes, forming a closed-loop iteration of "screening - evaluation - optimization - re-validation".

[0025] The new product finalization and batch efficacy verification module is used to complete the product preparation, pilot-scale verification, batch consistency testing, and efficacy verification of the optimized formula, ensuring stable product quality and reliable efficacy. It includes a product preparation unit, a pilot-scale verification unit, a batch consistency testing unit, and an efficacy verification unit. Product preparation unit: Prepare target products such as ginseng and astragalus oral liquid and ginseng and astragalus beverage according to the optimal formula and dosage form process; Pilot-scale verification unit: Complete the process scale-up from laboratory small-scale to pilot-scale, and verify the feasibility and stability of the process; Batch consistency testing unit: performs ingredient and efficacy testing on multiple batches of products to determine batch-to-batch differences; Efficacy verification unit: The efficacy of the finished product is retested using nematode high-throughput screening technology to verify that the product efficacy meets the standards.

[0026] The development method of a ginseng and astragalus health product development system using nematode high-throughput screening technology includes the following steps: Step 1: System initialization and nematode standardization preprocessing The standardized nematode culture and pretreatment module is activated. Synchronized nematode eggs are obtained through the synchronized hatching unit, cultured to the target instar in the isothermal culture unit, accurately sorted by the instar sorting unit, and sterile treatment unit removes contaminants to obtain standardized nematode samples. The system then enters the screening standby state.

[0027] Step 2: Sample pretreatment and gradient preparation of ginseng and astragalus The sample pretreatment and gradient preparation module for ginseng and astragalus is started. The raw material extraction unit extracts the active ingredients from the ginseng and astragalus raw materials. The purification and impurity removal unit removes impurities. The concentration and volume adjustment unit prepares the standard mother liquor. The gradient concentration preparation unit prepares multiple sets of gradient concentration samples, thus completing the sample preparation.

[0028] Step 3: High-throughput drug administration and environmental control The high-throughput nematode exposure and drug delivery module is activated. The high-throughput sample loading unit loads standardized nematodes and gradient concentration samples into a multi-well plate. The drug delivery unit completes standardized drug delivery. The exposure environment control unit maintains constant exposure conditions. The sealed light-proof unit ensures the stability of the exposure system. The exposure treatment is then initiated.

[0029] Step 4: Real-time collection of multi-dimensional efficacy indicators The multi-dimensional efficacy indicator real-time acquisition module is activated. The lifespan indicator acquisition unit records nematode survival data, the antioxidant indicator acquisition unit detects oxidative stress-related signals, the immune indicator acquisition unit monitors stress survival status, and the motility acquisition unit records movement parameters. Raw data is stored in real time. The average lifespan extension rate of nematodes is calculated based on the acquired data. .

[0030] Step 5: Efficacy Data Analysis and Comprehensive Evaluation The efficacy data analysis and comprehensive evaluation module is activated. The data noise reduction unit removes outlier data, the data normalization unit unifies the units, the efficacy feature extraction unit constructs a feature matrix, and the comprehensive score calculation unit calculates the comprehensive score of antioxidant activity. With the immune enhancement index Output the comprehensive efficacy evaluation results of the samples.

[0031] Step 6: Optimization and Iteration of Ginseng and Astragalus Product Formulas The formulation optimization and iteration module for ginseng and astragalus products is activated. The ratio optimization unit adjusts the ratio of ginseng and astragalus raw materials, the excipient screening unit screens suitable excipients, the dosage form adaptation unit matches the target dosage form, and the formulation iteration verification unit retests the optimized formulation to form the optimal formulation scheme.

[0032] Step 7: New Product Finalization and Batch Efficacy Validation The new product design and batch efficacy verification module is initiated. The product preparation unit prepares ginseng and astragalus finished products. The pilot verification unit completes process scale-up. The batch consistency testing unit tests the stability of multiple batches of products. The efficacy verification unit uses nematode high-throughput screening technology to complete the efficacy retest of the finished product. After confirming that the product efficacy meets the standards, the development is completed.

[0033] Step 8: System Archiving and Data Accumulation All screening data, evaluation results, formula parameters, and process parameters are archived and stored to form a database for the development of ginseng and astragalus health products, providing data support for subsequent product upgrades and new formula development.

[0034] Example 1: Development of Ginseng and Astragalus Oral Liquid Based on High-Throughput Screening of Nematodes Implementation process: Step 1: Preparation of standardized nematode samples The standardized nematode culture and pretreatment module was initiated. The synchronized hatching unit performed alkaline digestion and centrifugation purification on wild-type Caenorhabditis elegans eggs to obtain synchronized eggs. The isothermal culture unit placed the eggs in a standardized culture medium to maintain constant environmental parameters. Once the nematodes developed to the target instar, the instar sorting unit performed precise sorting based on body size and motility characteristics, removing individuals with abnormal development. The aseptic treatment unit used sterile reagents to sterilize the nematodes both on and inside their bodies, obtaining standardized nematode samples that were uniform and sterile, ensuring the consistency of the screened samples.

[0035] Step 2: Sample pretreatment and gradient preparation of ginseng and astragalus The sample pretreatment and gradient preparation module for ginseng and astragalus is activated. The raw material extraction unit uses authentic Codonopsis pilosula and Astragalus membranaceus as raw materials and employs a directional extraction process to enrich core active ingredients such as polysaccharides, verbascoside, and astragaloside A. The purification and impurity removal unit uses membrane separation technology to remove impurities such as starch, crude fiber, and pigments, thereby improving the purity of the extract. The concentration and volume adjustment unit concentrates the purified solution to a fixed concentration, and after volume adjustment, a standard mother liquor for ginseng and astragalus extraction is obtained. The gradient concentration preparation unit uses the standard mother liquor as a base and dilutes it stepwise in equal proportions to prepare multiple sets of gradient concentration samples. Each set is set up in parallel replicates to meet the requirements of high-throughput screening.

[0036] Step 3: High-throughput exposure treatment The high-throughput nematode exposure and drug delivery module is activated. The high-throughput sample loading unit dispenses standardized nematodes into a multi-well plate, with the same number of nematodes in each well. The drug delivery unit precisely adds ginseng and astragalus samples of different concentrations to the corresponding wells, while the blank control group is added with an equal amount of sterile solvent. The exposure environment control unit maintains constant environmental parameters throughout the exposure system, and the sealing and light-shielding unit seals and shields the multi-well plate from light to prevent sample degradation and contamination. Continuous exposure treatment is then initiated.

[0037] Step 4: Collection of multi-dimensional efficacy indicators A multi-dimensional efficacy indicator real-time acquisition module was activated. The lifespan indicator acquisition unit regularly observed the survival status of nematodes daily, recorded dead individuals, and statistically analyzed the average and maximum lifespan of the treatment and control groups. The antioxidant indicator acquisition unit detected reactive oxygen species levels and antioxidant enzyme-related signals in the nematodes, acquiring oxidative stress response data. The immune indicator acquisition unit placed the nematodes in a stress environment and monitored the survival ratio of the treatment and control groups. The locomotor ability acquisition unit recorded the nematode movement frequency and amplitude, reflecting physiological function. Based on the lifespan data, the average lifespan extension rate of the nematodes was calculated, quantifying the anti-aging efficacy.

[0038] Step 5: Efficacy Data Analysis and Comprehensive Evaluation The efficacy data analysis and comprehensive evaluation module is activated. The data noise reduction unit removes abnormal data during the experiment and smooths out data fluctuations. The data normalization unit converts different dimensional indicators such as life extension rate, reactive oxygen species level, stress survival rate, and exercise capacity into uniform range values. The efficacy feature extraction unit extracts the core features of anti-aging, anti-oxidation, and immune regulation and constructs an efficacy feature matrix. The comprehensive scoring calculation unit calculates the comprehensive score of antioxidant activity and the immune enhancement index respectively, comprehensively determines the efficacy level of the ginseng and astragalus samples, and determines the optimal efficacy concentration.

[0039] Step 6: Optimization and iteration of the formula for Ginseng and Astragalus Oral Liquid The formulation optimization and iteration module for the ginseng and astragalus product was initiated. The ratio optimization unit adjusted the quality ratio of Codonopsis pilosula and Astragalus membranaceus based on the optimal efficacy concentration, compared the comprehensive efficacy scores of different ratios, and determined the raw material ratio with the best efficacy. The excipient screening unit screened flavoring and stabilizing excipients that would not interfere with the core efficacy, based on the requirements of the oral liquid dosage form, and ensured that the excipients were compatible with the efficacy components of ginseng and astragalus. The dosage form adaptation unit determined the extraction, concentration, filtration, and filling process parameters of the oral liquid according to the characteristics of the formulation. The formulation iteration verification unit retested the nematode high-throughput efficacy of the optimized formulation to verify the stability of the formulation and form the final optimal formulation of ginseng and astragalus oral liquid.

[0040] Step 7: Product finalization and batch efficacy verification The new product design and batch efficacy verification module was launched. The product preparation unit prepared the finished ginseng and astragalus oral liquid according to the optimal formula and process parameters. The pilot-scale verification unit completed the process scale-up from laboratory small-scale to pilot-scale, verifying the feasibility of the process and the retention rate of components. The batch consistency detection unit conducted component testing on multiple batches of oral liquid to confirm that the component differences between batches met the requirements. The efficacy verification unit used nematode high-throughput screening technology to retest the efficacy of multiple batches of finished products, verifying that the anti-aging, antioxidant, and immune-enhancing effects of the finished product all met the standards, and that there were no significant differences in efficacy between batches, thus completing the development of ginseng and astragalus oral liquid.

[0041] Step 8: Data Archiving and Consolidation All nematode culture parameters, sample extraction parameters, gradient concentrations, exposure conditions, efficacy data, formula ratios, process parameters, and batch test results from this embodiment will be archived and stored to construct a dedicated database for the development of Shenqi oral liquid, providing data support for subsequent product upgrades and quality control.

[0042] Example 2: Development of Ginseng and Astragalus Beverage Based on High-Throughput Screening of Nematodes Implementation process: Step 1: Preparation of standardized nematode samples Using the same procedure as in Example 1, synchronized, sterile, and age-matched Caenorhabditis elegans samples were prepared through a standardized nematode culture and pretreatment module to ensure the consistency of the screening samples and eliminate the interference of individual differences on efficacy evaluation.

[0043] Step 2: Sample pretreatment and gradient preparation of ginseng and astragalus To meet the demand for refreshing, low-viscosity, and easy-to-drink beverage formulations, the raw material extraction unit employs a gentle extraction process to retain the aroma and active ingredients of ginseng and astragalus; the purification and impurity removal unit further removes large molecular impurities and improves the clarity of the extract; the concentration and volume adjustment unit prepares a standard mother liquor suitable for the beverage's flavor; and the gradient concentration preparation unit prepares multiple sets of low-concentration gradient samples to match the dosage requirements for daily beverage consumption, thus completing sample preparation.

[0044] Step 3: High-throughput exposure treatment Standardized nematode and ginseng gradient samples were loaded onto a high-throughput carrier. An exposure method matching the beverage drinking dose was adopted to maintain a stable exposure environment and complete the controllable exposure of nematode and ginseng samples, simulating the physiological exposure scenario of daily drinking.

[0045] Step 4: Collection of multi-dimensional efficacy indicators Real-time data on nematode lifespan, antioxidant capacity, immunity, and motility were collected, with a focus on efficacy response at low doses. The average lifespan extension rate was calculated to clarify the anti-aging, antioxidant, and immune-enhancing effects of ginseng and astragalus samples at beverage doses.

[0046] Step 5: Efficacy Data Analysis and Comprehensive Evaluation The collected data were denoised, normalized, and feature extracted. The antioxidant comprehensive score and immunity enhancement index were calculated to determine the efficacy stability of the ginseng and astragalus samples at low doses and to determine the optimal dosage for beverages.

[0047] Step 6: Optimization and iteration of the ginseng and astragalus beverage formula The formulation optimization unit adjusts the proportion of ginseng and astragalus extract to suit the refreshing taste of the beverage; the excipient screening unit screens suitable excipients such as carbonated and fruit-flavored ingredients to ensure that the excipients do not reduce the core efficacy of ginseng and astragalus; the dosage form adaptation unit determines the beverage's blending, homogenization, and sterilization processes; and the formula iteration verification unit verifies and optimizes the formula through nematode screening to ensure a balance between efficacy and taste.

[0048] Step 7: Product finalization and batch efficacy verification Pilot-scale samples of ginseng and astragalus beverage were prepared, and process scale-up and batch consistency testing were completed. High-throughput screening technology for nematodes was used to confirm that the efficacy of multiple batches of beverages was stable and met the requirements for health product efficacy, thus completing the development of ginseng and astragalus beverage.

[0049] Step 8: Data Archiving and Consolidation Archive the entire process data of ginseng and astragalus beverage development, integrate it with ginseng and astragalus oral liquid data to form a complete nematode screening and development system for ginseng and astragalus health products, supporting the development of multi-dosage form products.

[0050] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A ginseng and astragalus health product development system using high-throughput nematode screening technology, characterized in that: It includes modules for standardized nematode culture and pretreatment, ginseng and astragalus sample pretreatment and gradient formulation, high-throughput nematode exposure drug delivery, real-time acquisition of multi-dimensional efficacy indicators, efficacy data analysis and comprehensive evaluation, ginseng and astragalus product formula optimization and iteration, and new product finalization and batch efficacy verification. The standardized nematode culture and pretreatment module includes a synchronized hatching unit, a constant temperature culture unit, an instar sorting unit, and a sterile treatment unit, which are used to realize synchronized nematode culture, precise instar control, and sterile pretreatment. The ginseng and astragalus sample pretreatment and gradient preparation module includes a raw material extraction unit, a purification and impurity removal unit, a concentration and volume adjustment unit, and a gradient concentration preparation unit, which are used to complete the extraction, purification and gradient concentration preparation of ginseng and astragalus samples. The nematode high-throughput exposure drug delivery module includes a high-throughput sample loading unit, an exposure environment control unit, a drug delivery unit, and a sealed light-proof unit, which is used to achieve high-throughput controllable exposure of nematodes and ginseng and astragalus samples. The multi-dimensional efficacy indicator real-time acquisition module includes a lifespan indicator acquisition unit, an antioxidant indicator acquisition unit, an immune indicator acquisition unit, and a motor ability acquisition unit, which are used to collect phenotypic data related to anti-aging, antioxidant, and immune regulation in nematodes. The efficacy data analysis and comprehensive evaluation module includes a data noise reduction unit, a data normalization unit, an efficacy feature extraction unit, and a comprehensive score calculation unit, which are used to complete efficacy data processing and comprehensive scoring. The formulation optimization and iteration module for ginseng and astragalus products includes a ratio optimization unit, an excipient screening unit, a dosage form adaptation unit, and a formulation iteration verification unit, which are used to complete formulation optimization and iteration based on efficacy results. The new product finalization and batch efficacy verification module includes a product preparation unit, a pilot-scale verification unit, a batch consistency testing unit, and an efficacy verification unit, which are used to complete product finalization, process verification, and efficacy verification.

2. The ginseng and astragalus health product development system using nematode high-throughput screening technology according to claim 1, characterized in that: The nematode standardized culture and pretreatment module includes a synchronized hatching unit that uses a combination of alkali digestion and centrifugal purification to obtain synchronized eggs; a constant temperature culture unit that maintains constant culture environment parameters; an instar sorting unit that sorts nematodes at specific instars based on their body size and movement characteristics; and an aseptic treatment unit that removes bacteria from the nematode surface and inside the nematode.

3. The ginseng and astragalus health product development system using nematode high-throughput screening technology according to claim 1, characterized in that: The raw material extraction unit of the ginseng and astragalus sample pretreatment and gradient preparation module is used to enrich the polysaccharides, saponins, and flavonoids in the ginseng and astragalus raw materials; the purification and impurity removal unit is used to remove non-functional impurities; the concentration and volume adjustment unit is used to prepare standard mother liquor; and the gradient concentration preparation unit is used to prepare multiple sets of gradient concentration samples by step-by-step dilution.

4. The ginseng and astragalus health product development system using nematode high-throughput screening technology according to claim 1, characterized in that: The multi-dimensional efficacy indicator real-time acquisition module is configured with a nematode average lifespan extension rate calculation model, and the calculation formula is as follows: In the formula: The average lifespan extension rate of nematodes; The average lifespan of nematodes in the Astragalus and Ginseng sample treatment group; The average lifespan of nematodes represents the blank control group.

5. The ginseng and astragalus health product development system using nematode high-throughput screening technology according to claim 1, characterized in that: The efficacy data analysis and comprehensive evaluation module is configured with an antioxidant activity comprehensive scoring calculation model, and the calculation formula is as follows: In the formula: The overall score is based on antioxidant activity. The number of antioxidant evaluation indicators; For the first Weighting coefficients for antioxidant indicators; For the first Normalized values ​​of antioxidant indicators.

6. The ginseng and astragalus health product development system using nematode high-throughput screening technology according to claim 1, characterized in that: The efficacy data analysis and comprehensive evaluation module is equipped with an immunity enhancement index calculation model, and the calculation formula is as follows: In the formula: An index for enhancing immunity; The survival rate of nematodes under stress in the ginseng and astragalus sample treatment group; The stress survival rate of nematodes in the blank control group; This is the system error correction coefficient.

7. The ginseng and astragalus health product development system using nematode high-throughput screening technology according to claim 1, characterized in that: The formulation optimization and iteration module for the ginseng and astragalus products is guided by the efficacy evaluation results, and completes the adjustment of the ratio of codonopsis and astragalus, screening of excipients, adaptation of dosage forms, and closed-loop iterative verification of the formulation.

8. The ginseng and astragalus health product development system using nematode high-throughput screening technology according to claim 1, characterized in that: The new product finalization and batch efficacy verification module uses nematode high-throughput screening technology to complete the efficacy retesting of finished product batches, ensuring the consistency of efficacy across multiple batches of products.

9. The development method of the ginseng and astragalus health product development system using nematode high-throughput screening technology according to any one of claims 1-8, characterized in that: Includes the following steps: Step 1: Start the standardized nematode culture and pretreatment module to prepare standardized sterile nematode samples; Step 2: Start the ginseng and astragalus sample pretreatment and gradient preparation module to complete the extraction of ginseng and astragalus samples and the preparation of gradient concentrations; Step 3: Activate the nematode high-throughput exposure drug delivery module to complete the high-throughput exposure treatment of nematodes and ginseng and astragalus samples; Step 4: Activate the multi-dimensional efficacy indicator real-time acquisition module to collect multi-dimensional efficacy data and calculate the average life extension rate of nematodes; Step 5: Activate the efficacy data analysis and comprehensive evaluation module to complete data processing and calculate the comprehensive score of antioxidant activity and the immunity enhancement index; Step 6: Activate the formulation optimization and iteration module for ginseng and astragalus products, and complete formulation optimization and iteration verification based on efficacy results; Step 7: Activate the new product finalization and batch efficacy verification module to complete product preparation, pilot testing, batch testing and efficacy verification; Step 8: Archive all development process data in the system to form a product development database.

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