Restrictive epitope peptide, nucleic acid molecule and application

By expressing chicken MHC I molecules in mammalian cell lines and screening for chicken MHC I molecule BF2*1901 restricted avian influenza virus CTL epitopes, the high cost and low efficiency of traditional methods were solved, and immunogenic peptides were screened out, which are suitable for avian influenza virus vaccine design and immune mechanism research.

CN122444830APending Publication Date: 2026-07-24CHINA AGRI UNIV
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Patent Information

Application Number
CN202610221101.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-08
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing technologies are difficult to efficiently screen for chicken MHC I molecule BF2*1901 restricted avian influenza virus CTL epitopes. Traditional methods require a large number of haplotype chicken immune cells and are costly. They are also prone to misidentification or missed detection of weakly binding peptides that do not conform to the motif and ultrashort peptides of atypical length.

Method used

Using mammalian cells as the transfection target, a cell line expressing chicken MHC I molecules was constructed. MHC I-peptide complexes were prepared by viral infection, and 23 chicken MHC I molecule BF2*1901 restricted avian influenza virus CTL epitopes were screened by replacing chicken MHC I monoclonal antibodies with tag antibodies.

Benefits of technology

This method enables rapid, economical, and effective screening of CTL epitopes, reducing costs and identifying 21 newly identified CTL epitopes applicable to different chicken MHC I molecules and subtypes of avian influenza viruses, providing a scientific basis for vaccine design and immunization evaluation.

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Abstract

The invention belongs to the field of biology, and discloses an AIV (avian influenza virus) MHC-BF2 * 1901 restrictive epitope peptide, and the amino acid sequence of the epitope peptide is as shown in any one of SEQ ID NO.1, SEQ ID NO.3-SEQ ID NO.18. The invention also discloses a preparation method of the AIV MHC-BF2 * 1901 restrictive epitope peptide. According to the invention, 23 chicken MHC I molecule BF2 * 1901 restricted avian influenza virus CTLs are successfully screened, wherein 21 peptides are CTL epitopes newly identified by the research. According to the method, weak binding peptides which do not conform to the motif and ultra-short peptides with atypical lengths can be identified, and the peptide fragments are easily misjudged or missed by a traditional method, but have immunogenicity. Meanwhile, the limitation that a large number of haplotype chicken immune cells are needed in a traditional screening method is solved, the CTL epitope screening cost is remarkably reduced by replacing a chicken MHC I monoclonal antibody with a tag antibody, and a rapid, economical and effective CTL epitope screening technology is provided for avian influenza virus vaccine design and research of a cellular immune mechanism. Meanwhile, the invention further discloses a nucleic acid molecule and application.
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